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23 results on '"Gonzalez-Nieto, Lucas"'

1. Induction of Transient Virus Replication Facilitates Antigen-Independent Isolation of SIV-Specific Monoclonal Antibodies

Author

Pedreño-Lopez, Nuria, Dang, Christine M., Rosen, Brandon C., Ricciardi, Michael J., Bailey, Varian K., Gutman, Martin J., Gonzalez-Nieto, Lucas, Pauthner, Matthias G., Le, Khoa, Song, Ge, Andrabi, Raiees, Weisgrau, Kim L., Pomplun, Nicholas, Martinez-Navio, José M., Fuchs, Sebastian P., Wrammert, Jens, Rakasz, Eva G., Lifson, Jeffrey D., Martins, Mauricio A., Burton, Dennis R., Watkins, David I., and Magnani, Diogo M.

Published
2020
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2. Vaccine protection against SIVmac239 acquisition

Author

Martins, Mauricio A., Bischof, Georg F., Shin, Young C., Lauer, William A., Gonzalez-Nieto, Lucas, Watkins, David I., Rakasz, Eva G., Lifson, Jeffrey D., and Desrosiers, Ronald C.

Published
2019

3. Dengue Virus Evades AAV-Mediated Neutralizing Antibody Prophylaxis in Rhesus Monkeys

Author

Magnani, Diogo M., Ricciardi, Michael J., Bailey, Varian K., Gutman, Martin J., Pedreño-Lopez, Núria, Silveira, Cassia G.T., Maxwell, Helen S., Domingues, Aline, Gonzalez-Nieto, Lucas, Su, Qin, Newman, Ruchi M., Pack, Melissa, Martins, Mauricio A., Martinez-Navio, José M., Fuchs, Sebastian P., Rakasz, Eva G., Allen, Todd M., Whitehead, Stephen S., Burton, Dennis R., Gao, Guangping, Desrosiers, Ronald C., Kallas, Esper G., and Watkins, David I.

Published
2017
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4. Therapeutic neutralizing monoclonal antibody administration protects against lethal yellow fever virus infection

Author

Ricciardi, Michael J., primary, Rust, Lauren N., additional, Pedreño-Lopez, Nuria, additional, Yusova, Sofiya, additional, Biswas, Sreya, additional, Webb, Gabriela M., additional, Gonzalez-Nieto, Lucas, additional, Voigt, Thomas B., additional, Louw, Johan J., additional, Laurino, Fernanda D., additional, DiBello, John R., additional, Raué, Hans-Peter, additional, Barber-Axthelm, Aaron M., additional, Chun, Kimberly, additional, Uttke, Samantha, additional, Raphael, Lidiane M. S., additional, Yrizarry-Medina, Aaron, additional, Rosen, Brandon C., additional, Agnor, Rebecca, additional, Gao, Lina, additional, Labriola, Caralyn, additional, Axthelm, Michael, additional, Smedley, Jeremy, additional, Julander, Justin G., additional, Bonaldo, Myrna C., additional, Walker, Laura M., additional, Messaoudi, Ilhem, additional, Slifka, Mark K., additional, Burton, Dennis R., additional, Kallas, Esper G., additional, Sacha, Jonah B., additional, Watkins, David I., additional, and Burwitz, Benjamin J., additional

Published
2023
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5. Neutralizing human monoclonal antibodies prevent Zika virus infection in macaques

Author

Magnani, Diogo M., Rogers, Thomas F., Beutler, Nathan, Ricciardi, Michael J., Bailey, Varian K., Gonzalez-Nieto, Lucas, Briney, Bryan, Sok, Devin, Le, Khoa, Strubel, Alexander, Gutman, Martin J., Pedreño-Lopez, Núria, Grubaugh, Nathan D., Silveira, Cassia G. T., Maxwell, Helen S., Domingues, Aline, Martins, Mauricio A., Lee, David E., Okwuazi, Erica E., Jean, Sherrie, Strobert, Elizabeth A., Chahroudi, Ann, Silvestri, Guido, Vanderford, Thomas H., Kallas, Esper G., Desrosiers, Ronald C., Bonaldo, Myrna C., Whitehead, Stephen S., Burton, Dennis R., and Watkins, David I.

Published
2017
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7. Rectal Acquisition of Simian Immunodeficiency Virus (SIV) SIVmac239 Infection despite Vaccine-Induced Immune Responses against the Entire SIV Proteome

Author

Martins, Mauricio A., primary, Gonzalez-Nieto, Lucas, additional, Ricciardi, Michael J., additional, Bailey, Varian K., additional, Dang, Christine M., additional, Bischof, Georg F., additional, Pedreño-Lopez, Nuria, additional, Pauthner, Matthias G., additional, Burton, Dennis R., additional, Parks, Christopher L., additional, Earl, Patricia, additional, Moss, Bernard, additional, Rakasz, Eva G., additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, and Watkins, David I., additional

Published
2020
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8. A Recombinant Rhesus Monkey Rhadinovirus Deleted of Glycoprotein L Establishes Persistent Infection of Rhesus Macaques and Elicits Conventional T Cell Responses

Author

Hahn, Alexander S., primary, Bischof, Georg F., additional, Großkopf, Anna K., additional, Shin, Young C., additional, Domingues, Aline, additional, Gonzalez-Nieto, Lucas, additional, Rakasz, Eva G., additional, Watkins, David I., additional, Ensser, Armin, additional, Martins, Mauricio A., additional, and Desrosiers, Ronald C., additional

Published
2020
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9. Vaccine protection against rectal acquisition of SIVmac239 in rhesus macaques

Author

Gonzalez-Nieto, Lucas, primary, Castro, Isabelle M., additional, Bischof, Georg F., additional, Shin, Young C., additional, Ricciardi, Michael J., additional, Bailey, Varian K., additional, Dang, Christine M., additional, Pedreño-Lopez, Nuria, additional, Magnani, Diogo M., additional, Ejima, Keisuke, additional, Allison, David B., additional, Gil, Hwi Min, additional, Evans, David T., additional, Rakasz, Eva G., additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, and Martins, Mauricio A., additional

Published
2019
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10. The Frequency of Vaccine-Induced T-Cell Responses Does Not Predict the Rate of Acquisition after Repeated Intrarectal SIVmac239 Challenges inMamu-B*08 +Rhesus Macaques

Author

Martins, Mauricio A., primary, Gonzalez-Nieto, Lucas, additional, Shin, Young C., additional, Domingues, Aline, additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Magnani, Diogo M., additional, Ricciardi, Michael J., additional, Pedreño-Lopez, Núria, additional, Bailey, Varian K., additional, Altman, John D., additional, Parks, Christopher L., additional, Allison, David B., additional, Ejima, Keisuke, additional, Rakasz, Eva G., additional, Capuano, Saverio, additional, Desrosiers, Ronald C., additional, Lifson, Jeffrey D., additional, and Watkins, David I., additional

Published
2019
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11. Mamu-B*17 + Rhesus Macaques Vaccinated with env , vif , and nef Manifest Early Control of SIVmac239 Replication

Author

Martins, Mauricio A., primary, Tully, Damien C., additional, Pedreño-Lopez, Núria, additional, von Bredow, Benjamin, additional, Pauthner, Matthias G., additional, Shin, Young C., additional, Yuan, Maoli, additional, Lima, Noemia S., additional, Bean, David J., additional, Gonzalez-Nieto, Lucas, additional, Domingues, Aline, additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Magnani, Diogo M., additional, Ricciardi, Michael J., additional, Bailey, Varian K., additional, Altman, John D., additional, Burton, Dennis R., additional, Ejima, Keisuke, additional, Allison, David B., additional, Evans, David T., additional, Rakasz, Eva G., additional, Parks, Christopher L., additional, Bonaldo, Myrna C., additional, Capuano, Saverio, additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, Allen, Todd M., additional, and Watkins, David I., additional

Published
2018
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12. A recombinant herpesviral vector containing a near-full-length SIVmac239 genome produces SIV particles and elicits immune responses to all nine SIV gene products

Author

Shin, Young C., primary, Bischof, Georg F., additional, Lauer, William A., additional, Gonzalez-Nieto, Lucas, additional, Rakasz, Eva G., additional, Hendricks, Gregory M., additional, Watkins, David I., additional, Martins, Mauricio A., additional, and Desrosiers, Ronald C., additional

Published
2018
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13. Fetal demise and failed antibody therapy during Zika virus infection of pregnant macaques

Author

Magnani, Diogo M., primary, Rogers, Thomas F., additional, Maness, Nicholas J., additional, Grubaugh, Nathan D., additional, Beutler, Nathan, additional, Bailey, Varian K., additional, Gonzalez-Nieto, Lucas, additional, Gutman, Martin J., additional, Pedreño-Lopez, Núria, additional, Kwal, Jaclyn M., additional, Ricciardi, Michael J., additional, Myers, Tereance A., additional, Julander, Justin G., additional, Bohm, Rudolf P., additional, Gilbert, Margaret H., additional, Schiro, Faith, additional, Aye, Pyone P., additional, Blair, Robert V., additional, Martins, Mauricio A., additional, Falkenstein, Kathrine P., additional, Kaur, Amitinder, additional, Curry, Christine L., additional, Kallas, Esper G., additional, Desrosiers, Ronald C., additional, Goldschmidt-Clermont, Pascal J., additional, Whitehead, Stephen S., additional, Andersen, Kristian G., additional, Bonaldo, Myrna C., additional, Lackner, Andrew A., additional, Panganiban, Antonito T., additional, Burton, Dennis R., additional, and Watkins, David I., additional

Published
2018
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14. Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Author

Gonzalez-Nieto, Lucas, Domingues, Aline, Ricciardi, Michael, Gutman, Martin J., Maxwell, Helen S., Pedreño-Lopez, Nuria, Bailey, Varian, Magnani, Diogo M., and Martins, Mauricio A.

Subjects
Staining and Labeling, Immunology, Histocompatibility Antigens Class I, Animals, Simian Immunodeficiency Virus, CD8-Positive T-Lymphocytes, Flow Cytometry, Peptides, Macaca mulatta, Immunophenotyping
Abstract

Peptide-major histocompatibility complex class I (pMHC-I) tetramers have been an invaluable tool to study CD8+ T-cell responses. Because these reagents directly bind to T-cell receptors on the surface of CD8+ T-lymphocytes, fluorochrome-labeled pMHC-I tetramers enable the accurate detection of antigen (Ag)-specific CD8+ T-cells without the need for in vitro re-stimulation. Moreover, when combined with multi-color flow cytometry, pMHC-I tetramer staining can reveal key aspects of Ag-specific CD8+ T-cells, including differentiation stage, memory phenotype, and activation status. These types of analyses have been especially useful in the field of HIV immunology where CD8+ T-cells can affect progression to AIDS. Experimental infection of rhesus macaques with simian immunodeficiency virus (SIV) provides an invaluable tool to study cellular immunity against the AIDS virus. As a result, considerable progress has been made in defining and characterizing T-cell responses in this animal model. Here we present an optimized protocol for enumerating SIV-specific CD8+ T-cells in rhesus macaques by pMHC-I tetramer staining. Our assay permits the simultaneous quantification and memory phenotyping of two pMHC-I tetramer+ CD8+ T-cell populations per test, which might be useful for tracking SIV-specific CD8+ T-cell responses generated by vaccination or SIV infection. Considering the relevance of nonhuman primates in biomedical research, this methodology is applicable for studying CD8+ T-cell responses in multiple disease settings.

Published
2016

15. Ontogeny of the B- and T-cell response in a primary Zika virus infection of a dengue-naïve individual during the 2016 outbreak in Miami, FL

Author

Ricciardi, Michael J., primary, Magnani, Diogo M., additional, Grifoni, Alba, additional, Kwon, Young-Chan, additional, Gutman, Martin J., additional, Grubaugh, Nathan D., additional, Gangavarapu, Karthik, additional, Sharkey, Mark, additional, Silveira, Cassia G. T., additional, Bailey, Varian K., additional, Pedreño-Lopez, Núria, additional, Gonzalez-Nieto, Lucas, additional, Maxwell, Helen S., additional, Domingues, Aline, additional, Martins, Mauricio A., additional, Pham, John, additional, Weiskopf, Daniela, additional, Altman, John, additional, Kallas, Esper G., additional, Andersen, Kristian G., additional, Stevenson, Mario, additional, Lichtenberger, Paola, additional, Choe, Hyeryun, additional, Whitehead, Stephen S., additional, Sette, Alessandro, additional, and Watkins, David I., additional

Published
2017
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16. Potent Plasmablast-Derived Antibodies Elicited by the National Institutes of Health Dengue Vaccine

Author

Magnani, Diogo M., primary, Silveira, Cassia G. T., additional, Ricciardi, Michael J., additional, Gonzalez-Nieto, Lucas, additional, Pedreño-Lopez, Núria, additional, Bailey, Varian K., additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Domingues, Aline, additional, Costa, Priscilla R., additional, Ferrari, Lilian, additional, Goulart, Raphaella, additional, Martins, Mauricio A., additional, Martinez-Navio, José M., additional, Fuchs, Sebastian P., additional, Kalil, Jorge, additional, Timenetsky, Maria do Carmo, additional, Wrammert, Jens, additional, Whitehead, Stephen S., additional, Burton, Dennis R., additional, Desrosiers, Ronald C., additional, Kallas, Esper G., additional, and Watkins, David I., additional

Published
2017
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18. Rare Control of SIVmac239 Infection in a Vaccinated Rhesus Macaque

Author

Martins, Mauricio A., primary, Tully, Damien C., additional, Shin, Young C., additional, Gonzalez-Nieto, Lucas, additional, Weisgrau, Kim L., additional, Bean, David J., additional, Gadgil, Rujuta, additional, Gutman, Martin J., additional, Domingues, Aline, additional, Maxwell, Helen S., additional, Magnani, Diogo M., additional, Ricciardi, Michael, additional, Pedreño-Lopez, Nuria, additional, Bailey, Varian, additional, Cruz, Michael A., additional, Lima, Noemia S., additional, Bonaldo, Myrna C., additional, Altman, John D., additional, Rakasz, Eva, additional, Capuano, Saverio, additional, Reimann, Keith A., additional, Piatak, Michael, additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, Allen, Todd M., additional, and Watkins, David I., additional

Published
2017
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19. Vaccine-induced immune responses against both Gag and Env improve control of simian immunodeficiency virus replication in rectally challenged rhesus macaques

Author

Martins, Mauricio A., primary, Shin, Young C., additional, Gonzalez-Nieto, Lucas, additional, Domingues, Aline, additional, Gutman, Martin J., additional, Maxwell, Helen S., additional, Castro, Iris, additional, Magnani, Diogo M., additional, Ricciardi, Michael, additional, Pedreño-Lopez, Nuria, additional, Bailey, Varian, additional, Betancourt, Dillon, additional, Altman, John D., additional, Pauthner, Matthias, additional, Burton, Dennis R., additional, von Bredow, Benjamin, additional, Evans, David T., additional, Yuan, Maoli, additional, Parks, Christopher L., additional, Ejima, Keisuke, additional, Allison, David B., additional, Rakasz, Eva, additional, Barber, Glen N., additional, Capuano, Saverio, additional, Lifson, Jeffrey D., additional, Desrosiers, Ronald C., additional, and Watkins, David I., additional

Published
2017
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20. A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus

Author

Magnani, Diogo M., primary, Silveira, Cassia G. T., additional, Rosen, Brandon C., additional, Ricciardi, Michael J., additional, Pedreño-Lopez, Núria, additional, Gutman, Martin J., additional, Bailey, Varian K., additional, Maxwell, Helen S., additional, Domingues, Aline, additional, Gonzalez-Nieto, Lucas, additional, Avelino-Silva, Vivian I., additional, Trindade, Mateus, additional, Nogueira, Juliana, additional, Oliveira, Consuelo S., additional, Maestri, Alvino, additional, Felix, Alvina Clara, additional, Levi, José Eduardo, additional, Nogueira, Mauricio L., additional, Martins, Mauricio A., additional, Martinez-Navio, José M., additional, Fuchs, Sebastian P., additional, Whitehead, Stephen S., additional, Burton, Dennis R., additional, Desrosiers, Ronald C., additional, Kallas, Esper G., additional, and Watkins, David I., additional

Published
2017
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21. The Frequency of Vaccine-Induced T-Cell Responses Does Not Predict the Rate of Acquisition after Repeated Intrarectal SIVmac239 Challenges in Mamu-B*08+ Rhesus Macaques.

Author

Martins, Mauricio A., Gonzalez-Nieto, Lucas, Shin, Young C., Domingues, Aline, Gutman, Martin J., Maxwell, Helen S., Magnani, Diogo M., Ricciardi, Michael J., Pedreño-Lopez, Núria, Bailey, Varian K., Altman, John D., Parks, Christopher L., Allison, David B., Ejima, Keisuke, Rakasz, Eva G., Capuano III, Saverio, Desrosiers, Ronald C., Lifson, Jeffrey D., and Watkins, David I.

Subjects
*RHESUS monkeys, *SIMIAN immunodeficiency virus diseases vaccines, *T cells, *CD8 antigen, *HIV infections
Abstract

Approximately 50% of rhesus macaques (RMs) expressing the major histocompatibility complex class I (MHC-I) allele Mamu-B*08 spontaneously control chronic-phase viremia after infection with the pathogenic simian immunodeficiency virus mac239 (SIVmac239) clone. CD8+ T-cell responses in these animals are focused on immunodominant Mamu-B*08-restricted SIV epitopes in Vif and Nef, and prophylactic vaccination with these epitopes increases the incidence of elite control in SIVmac239-infected Mamu-B*08-positive (Mamu-B*08+) RMs. Here we evaluated if robust vaccine-elicited CD8+ T-cell responses against Vif and Nef can prevent systemic infection in Mamu-B*08+ RMs following mucosal SIV challenges. Ten Mamu-B*08+ RMs were vaccinated with a heterologous prime/boost/boost regimen encoding Vif and Nef, while six sham-vaccinated MHC-I-matched RMs served as the controls for this experiment. Vaccine-induced CD8+ T cells against Mamu-B*08-restricted SIV epitopes reached high frequencies in blood but were present at lower levels in lymph node and gut biopsy specimens. Following repeated intrarectal challenges with SIVmac239, all control RMs became infected by the sixth SIV exposure. By comparison, four vaccinees were still uninfected after six challenges, and three of them remained aviremic after 3 or 4 additional challenges. The rate of SIV acquisition in the vaccinees was numerically lower (albeit not statistically significantly) than that in the controls. However, peak viremia was significantly reduced in infected vaccinees compared to control animals. We found no T-cell markers that distinguished vaccinees that acquired SIV infection from those that did not. Additional studies will be needed to validate these findings and determine if cellular immunity can be harnessed to prevent the establishment of productive immunodeficiency virus infection. [ABSTRACT FROM AUTHOR]

Published
2019
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23. Mamu-B*17+ Rhesus Macaques Vaccinated with env, vif, and nef Manifest Early Control of SIVmac239 Replication.

Author

Martins, Mauricio A., Tully, Damien C., Pedreño-Lopez, Núria, Von Bredow, Benjamin, Pauthner, Matthias G., Shin, Young C., Yuan, Maoli, Lima, Noemia S., Bean, David J., Gonzalez-Nieto, Lucas, Domingues, Aline, Gutman, Martin J., Maxwell, Helen S., Magnani, Diogo M., Ricciardi, Michael J., Bailey, Varian K., Altman, John D., Burton, Dennis R., Ejima, Keisuke, and Allison, David B.

Subjects
*RHESUS monkeys, *MAJOR histocompatibility complex, *ANIMAL vaccination, *SIMIAN immunodeficiency virus diseases, *HIV prevention
Abstract

Certain major histocompatibility complex class I (MHC-I) alleles are associated with spontaneous control of viral replication in human immunodeficiency virus (HIV)-infected people and simian immunodeficiency virus (SIV)- infected rhesus macaques (RMs). These cases of "elite" control of HIV/SIV replication are often immune-mediated, thereby providing a framework for studying anti-lentiviral immunity. In this study, we examined how vaccination impacts SIV replication in RMs expressing the MHC-I allele Mamu-B*17. Approximately 21% of Mamu-B*17+ and 50% of Mamu-B*08+ RMs control chronic-phase viremia after SIVmac239 infection. Because CD8+ T cells targeting Mamu-B*08-restricted SIV epitopes have been implicated in virologic suppression in Mamu-B*08+ RMs, we investigated whether this might also be true for Mamu-B*17+ RMs. Two groups of Mamu-B*17+ RMs were vaccinated with genes encoding Mamu-B*17-restricted epitopes in Vif and Nef. These genes were delivered by themselves (group 1) or together with env (group 2). Group 3 included MHC-I-matched RMs and served as the control group. Surprisingly, the group 1 vaccine regimen had little effect on viral replication compared to group 3, suggesting that unlike Mamu-B*08+ RMs, preexisting SIV-specific CD8+ T cells alone do not facilitate long-term virologic suppression in Mamu-B*17+ RMs. Remarkably, however, 5/8 group 2 vaccinees controlled viremia to <15 viral RNA copies/ml soon after infection. No serological neutralizing activity against SIVmac239 was detected in group 2, although vaccine-elicited gp140-binding antibodies correlated inversely with nadir viral loads. Collectively, these data shed new light on the unique mechanism of elite control in Mamu-B*17+ RMs and implicate vaccine-induced, nonneutralizing anti- Env antibodies in the containment of immunodeficiency virus infection. [ABSTRACT FROM AUTHOR]

Published
2018
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