36 results on '"González-Miguéns, Rubén"'
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2. Deconstructing Difflugia: The tangled evolution of lobose testate amoebae shells (Amoebozoa: Arcellinida) illustrates the importance of convergent evolution in protist phylogeny
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González-Miguéns, Rubén, Todorov, Milcho, Blandenier, Quentin, Duckert, Clément, Porfirio-Sousa, Alfredo L., Ribeiro, Giulia M., Ramos, Diana, Lahr, Daniel J.G., Buckley, David, and Lara, Enrique
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- 2022
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3. The voice of the little giants: Arcellinida testate amoebae in environmental DNA‐based bioindication, from taxonomy free to haplotypic level.
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González‐Miguéns, Rubén, Cano, Emilio, García‐Gallo Pinto, Mónica, Peña, Pablo G., Rincón‐Barrado, Mario, Iglesias, Guillermo, Blanco‐Rotea, Andrés, Carrasco‐Braganza, María Isabel, de Salvador‐Velasco, David, Guillén‐Oterino, Antonio, Tenorio‐Rodríguez, Daniel, Siemensma, Ferry, Velázquez, David, and Lara, Enrique
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MICROBIOLOGY , *ECOLOGICAL disturbances , *TOP predators , *CONSERVATION biology , *ENVIRONMENTAL protection - Abstract
Bioindication, evaluating biological responses to environmental disturbances, is crucial for assessing the ecological status of an ecosystem. While historical bioindication relied on macroscopic organisms, the introduction of environmental DNA (eDNA) techniques allows the application of protists without the necessity of morphological identification. In this study, we propose a novel bioindication methodology utilizing Arcellinida, a group of top predators among protists, as bioindicators of freshwater ecosystems. For that purpose, we first characterized the Arcellinida diversity over 1 year at three different points of Lake Sanabria, an ancient glacier lake known to be subjected to anthropogenic disturbances. We compared this diversity with an undisturbed control site. Second, we characterized the Arcellinida diversity in other ecosystems to generate the ecological background to test the connectivity between them. Results indicate limited connectivity between the different ecosystems and an edge effect between terrestrial and aquatic ecosystems. Disturbed freshwater ecosystems exhibited reduced Arcellinida diversity at both specific and infraspecific levels, providing valuable insight into recent disturbances. Arcellinida‐based bioindication provides a sensitive, accurate and easy‐to‐interpret protocol for monitoring disturbances in freshwater ecosystems. It represents a valuable tool for environmental assessments and conservation strategies. [ABSTRACT FROM AUTHOR]
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- 2024
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4. When ecological transitions are not so infrequent: independent colonizations of athalassohaline water bodies by Arcellidae (Arcellinida; Amoebozoa), with descriptions of four new species
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Useros, Fernando, primary, González-Miguéns, Rubén, additional, Soler-Zamora, Carmen, additional, and Lara, Enrique, additional
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- 2023
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5. A needle in a haystack: A new metabarcoding approach to survey diversity at the species level of Arcellinida (Amoebozoa: Tubulinea)
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González‐Miguéns, Rubén, primary, Cano, Emilio, additional, Guillén‐Oterino, Antonio, additional, Quesada, Antonio, additional, Lahr, Daniel J. G., additional, Tenorio‐Rodríguez, Daniel, additional, de Salvador‐Velasco, David, additional, Velázquez, David, additional, Carrasco‐Braganza, María Isabel, additional, Patterson, R. Timothy, additional, Lara, Enrique, additional, and Singer, David, additional
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- 2023
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6. When ecological transitions are not so infrequent: independent colonizations of athalassohaline water bodies by Arcellidae (Arcellinida; Amoebozoa), with descriptions of four new species
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Useros, Fernando, González-Miguéns, Rubén, Soler-Zamora, Carmen, Lara, Enrique, Useros, Fernando, González-Miguéns, Rubén, Soler-Zamora, Carmen, and Lara, Enrique
- Abstract
The salinity and humidity barriers divide biodiversity and strongly influence the distribution of organisms. Crossing them opens the possibility for organisms to colonize new niches and diversify, but requires profound physiological adaptations and is supposed to happen rarely in evolutionary history. We tested the relative importance of each ecological barrier by building the phylogeny, based on mitochondrial cytochrome oxidase gene (COI) sequences, of a group of microorganisms common in freshwater and soils, the Arcellidae (Arcellinida; Amoebozoa). We explored the biodiversity of this family in the sediments of athalassohaline water bodies (i.e. of fluctuating salinity that have non-marine origins). We found three new aquatic species, which represent, to the best of our knowledge, the first reports of Arcellinida in these salt-impacted ecosystems, plus a fourth terrestrial one in bryophytes. Culturing experiments performed on Arcella euryhalina sp. nov. showed similar growth curves in pure freshwater and under 20 g/L salinity, and long-term survival at 50 g/L, displaying a halotolerant biology. Phylogenetic analyses showed that all three new athalassohaline species represent independent transition events through the salinity barrier by freshwater ancestor, in contrast to the terrestrial species, which are monophyletic and represent a unique ecological transition from freshwater to soil environments.
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- 2023
7. Expansion of the cytochrome C oxidase subunit I database and description of four new lobose testate amoebae species (Amoebozoa; Arcellinida)
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Ribeiro, G.M., Useros, Fernando, Dumack, K., González-Miguéns, Rubén, Siemensma, Ferry, Porfírio-Sousa, A.L., Soler-Zamora, Carmen, Pedro Barbosa Alcino, J., Lahr, D.J.G., Lara, Enrique, Ribeiro, G.M., Useros, Fernando, Dumack, K., González-Miguéns, Rubén, Siemensma, Ferry, Porfírio-Sousa, A.L., Soler-Zamora, Carmen, Pedro Barbosa Alcino, J., Lahr, D.J.G., and Lara, Enrique
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Arcellinida is ascending in importance in protistology, but description of their diversity still presents multiple challenges. Furthermore, applicable tools for surveillance of these organisms are still in developing stages. Importantly, a good database that sets a correspondence between molecular barcodes and species morphology is lacking. Cytochrome oxidase (COI) has been suggested as the most relevant marker for species discrimination in Arcellinida. However, some major groups of Arcellinida are still lacking a COI sequence. Here we expand the database of COI marker sequences for Arcellinids, using single-cell PCR, transcriptomics, and database scavenging. In the present work, we added 24 new Arcellinida COI sequences to the database, covering all unsampled infra- and suborders. Additionally, we added six new SSUrRNA sequences and described four new species using morphological, morphometrical, and molecular evidence: Heleopera steppica, Centropyxis blatta, Arcella uspiensis, and Cylindrifflugia periurbana. This new database will provide a new starting point to address new research questions from shell evolution, biogeography, and systematics of arcellinids.
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- 2023
8. A needle in a haystack: A new metabarcoding approach to survey diversity at the species level of Arcellinida (Amoebozoa: Tubulinea)
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González-Miguéns, Rubén, Cano, Emilio, Guillén-Oterino, Antonio, Quesada, Antonio, Lahr, Daniel J.G., Tenorio-Rodríguez, Daniel, de Salvador-Velasco, David, Velázquez, David, Carrasco-Braganza, María Isabel, Patterson, R. Timothy, Lara, Enrique, Singer, David, González-Miguéns, Rubén, Cano, Emilio, Guillén-Oterino, Antonio, Quesada, Antonio, Lahr, Daniel J.G., Tenorio-Rodríguez, Daniel, de Salvador-Velasco, David, Velázquez, David, Carrasco-Braganza, María Isabel, Patterson, R. Timothy, Lara, Enrique, and Singer, David
- Abstract
Environmental DNA-based diversity studies have increased in popularity with the development of high throughput sequencing technologies. This permits the potential simultaneous retrieval of vast amounts of molecular data from many different organisms and species, thus contributing to a wide range of biological disciplines. Environmental DNA protocols designed for protists often focused on the highly conserved small subunit of the ribosome gene, that does not permit species-level assignments. On the other hand, eDNA protocols aiming at species-level assignments allow a fine level ecological resolution and reproducible results. These protocols are currently applied to organisms living in marine and shallow lotic freshwater ecosystems, often in a bioindication purpose. Therefore, in this study, we present a species-level eDNA protocol designed to explore diversity of Arcellinida (Amoebozoa: Tubulinea) testate amoebae taxa that is based on mitochondrial cytochrome oxidase subunit I (COI). These organisms are widespread in lentic water bodies and soil ecosystems. We applied this protocol to 42 samples from peatlands, estuaries and soil environments, recovering all the infraorders in Glutinoconcha (with COI data), except for Hyalospheniformes. Our results revealed an unsuspected diversity in morphologically homogeneous groups such as Cylindrothecina, Excentrostoma or Sphaerothecina. With this protocol we expect to revolutionize the design of modern distributional Arcellinida surveys. Our approach involves a rapid and cost-effective analysis of testate amoeba diversity living in contrasted ecosystems. Therefore, the order Arcellinida has the potential to be established as a model group for a wide range of theoretical and applied studies.
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- 2023
9. The problem of 'shadow species' as illustrated with the taxonomic hotchpotch Cyphoderia ampulla (Rhizaria: Cyphoderiidae).
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Soler-Zamora, Carmen, Useros, Fernando, González-Miguéns, Rubén, Gómez-Rodríguez, Pablo, and Lara, Enrique
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SPECIES ,MICROORGANISMS ,BIOGEOGRAPHY ,TAXONOMISTS - Abstract
Accurate species delimitation based on an integrative approach is indispensable for biodiversity evaluation. Accordingly, species that were formerly only described morphologically often need to be split into several evolutionary independent units. However, taxonomic actions often lag behind, even when the required data are already available. As a result, invalid species names are carried over the years, with negative implications on ecology, biogeography, and conservation; we designate these entities as 'shadow species'. This is particularly common in protists, due to the lack of specialized taxonomists and the difficulties of working with microscopic organisms. Here, we resolve the case of the testate amoeba shadow species Cyphoderia ampulla (Rhizaria: Cercozoa: Euglyphida: Cyphoderiidae), a known polyphyletic taxon. Purposely, we integrated the current ecological, genetic, and morphological data on the family Cyphoderiidae with its described evolutionary history. Subsequently, we took the required taxonomic actions to resolve the taxonomy of the family, erecting four new genera (Psammoderia gen nov. Knarr gen. nov. Ichthyosquama gen. nov. and Oleiformis gen. nov.), emending Cyphoderia and describing four new species (Ichthyosquama sanabriensis sp. nov. , Ichthyosquama catoirensis sp. nov. , Ichthyosquama loricaria sp. nov. , and Oleiformis carmelae sp. nov.). Finally, we discuss the extension and relevance of the 'shadow species' issue in eukaryotic taxa, and differentiate it from species complex concept. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Deconstructing Difflugia: The tangled evolution of lobose testate amoebae shells (Amoebozoa: Arcellinida) illustrates the importance of convergent evolution in protist phylogeny
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Ministerio de Ciencia, Innovación y Universidades (España), González-Miguéns, Rubén, Todorov, Milcho, Blandenier, Quentin, Duckert, Clement, Porfirio-Sousa, Alfredo L., Ribeiro, Giulia M., Ramos, Diana, Lahr, Daniel J.G., Buckley, David, Enrique Lara, Ministerio de Ciencia, Innovación y Universidades (España), González-Miguéns, Rubén, Todorov, Milcho, Blandenier, Quentin, Duckert, Clement, Porfirio-Sousa, Alfredo L., Ribeiro, Giulia M., Ramos, Diana, Lahr, Daniel J.G., Buckley, David, and Enrique Lara
- Abstract
Protists, the micro-eukaryotes that are neither plants, animals nor fungi build up the greatest part of eukaryotic diversity on Earth. Yet, their evolutionary histories and patterns are still mostly ignored, and their complexity overlooked. Protists are often assumed to keep stable morphologies for long periods of time (morphological stasis). In this work, we test this paradigm taking Arcellinida testate amoebae as a model. We build a taxon-rich phylogeny based on two mitochondrial (COI and NADH) and one nuclear (SSU) gene, and reconstruct morphological evolution among clades. In addition, we prove the existence of mitochondrial mRNA editing for the COI gene. The trees show a lack of conservatism of shell outlines within the main clades, as well as a widespread occurrence of morphological convergences between far-related taxa. Our results refute, therefore, a widespread morphological stasis, which may be an artefact resulting from low taxon coverage. As a corollary, we also revise the groups systematics, notably by emending the large and highly polyphyletic genus Difflugia. These results lead, amongst others, to the erection of a new infraorder Cylindrothecina, as well as two new genera Cylindrifflugia and Golemanskia.
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- 2022
11. Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology
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Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-DePablo, Mar, Todorov, Milcho, Lara, Enrique, Ministerio de Ciencia e Innovación (España), Comunidad de Madrid, González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-DePablo, Mar, Todorov, Milcho, and Lara, Enrique
- Abstract
Protists are probably the most species-rich eukaryotes, yet their systematics are inaccurate, leading to an underestimation of their actual diversity. Arcellinida (= lobose testate amoebae) are amoebozoans that build a test (a hard shell) whose shape and composition are taxonomically informative. One of the most successful groups is Arcellidae, a family found worldwide in many freshwater and terrestrial environments where they are indicators of environmental quality. However, the systematics of the family is based on works published nearly a century ago. We re-evaluated the systematics based on single-cell barcoding, morphological and ecological data. Overall, test shape appears to be more related to environmental characteristics than to the species’ phylogenetic position. We show several convergences in organisms with similar ecology, some traditionally described species being paraphyletic. Based on conservative traits, we review the synapomorphies of the infraorder Sphaerothecina, compile a list of synonyms and describe a new genus Galeripora, with five new combinations. Seven new species: Arcella guadarramensis sp. nov., Galeripora balari sp. nov., Galeripora bufonipellita sp. nov., Galeripora galeriformis sp. nov., Galeripora naiadis sp. nov., Galeripora sitiens sp. nov. and Galeripora succelli sp. nov. are also described here.
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- 2022
12. Cyphoderia ampulla (Cyphoderiidae: Rhizaria), a tale of freshwater sailors: The causes and consequences of ecological transitions through the salinity barrier in a family of benthic protists
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Ministerio de Economía, Industria y Competitividad (España), González-Miguéns, Rubén, Soler-Zamora, Carmen, Useros López, Fernando, Nogal-Prata, Sandra, Berney, C., Blanco-Rotea, A., Carrasco-Braganza, M.I., de Salvador-Velasco, D., Guillén-Oterino, Antonio, Tenorio-Rodríguez, D., Velázquez, D., Heger, T.J., Sanmartín, Isabel, Lara, Enrique, Ministerio de Economía, Industria y Competitividad (España), González-Miguéns, Rubén, Soler-Zamora, Carmen, Useros López, Fernando, Nogal-Prata, Sandra, Berney, C., Blanco-Rotea, A., Carrasco-Braganza, M.I., de Salvador-Velasco, D., Guillén-Oterino, Antonio, Tenorio-Rodríguez, D., Velázquez, D., Heger, T.J., Sanmartín, Isabel, and Lara, Enrique
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The salinity barrier that separates marine and freshwater biomes is probably the most important division in biodiversity on Earth. Those organisms that successfully performed this transition had access to new ecosystems while undergoing changes in selective pressure, which often led to major shifts in diversification rates. While these transitions have been extensively investigated in animals, the tempo, mode, and outcome of crossing the salinity barrier have been scarcely studied in other eukaryotes. Here, we reconstructed the evolutionary history of the species complex Cyphoderia ampulla (Euglyphida: Cercozoa: Rhizaria) based on DNA sequences from the nuclear SSU rRNA gene and the mitochondrial cytochrome oxidase subunit I gene, obtained from publicly available environmental DNA data (GeneBank, EukBank) and isolated organisms. A tree calibrated with euglyphid fossils showed that four independent transitions towards freshwater systems occurred from the mid-Miocene onwards, coincident with important fluctuations in sea level. Ancestral trait reconstructions indicated that the whole family Cyphoderiidae had a marine origin and suggest that ancestors of the freshwater forms were euryhaline and lived in environments with fluctuating salinity. Diversification rates did not show any obvious increase concomitant with ecological transitions, but morphometric analyses indicated that species increased in size and homogenized their morphology after colonizing the new environments. This suggests adaptation to changes in selective pressure exerted by life in freshwater sediments.
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- 2022
13. Cyphoderia ampulla (Cyphoderiidae: Rhizaria), a tale of freshwater sailors: The causes and consequences of ecological transitions through the salinity barrier in a family of benthic protists
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González‐Miguéns, Rubén, primary, Soler‐Zamora, Carmen, additional, Useros, Fernando, additional, Nogal‐Prata, Sandra, additional, Berney, Cédric, additional, Blanco‐Rotea, Andrés, additional, Carrasco‐Braganza, María Isabel, additional, Salvador‐Velasco, David, additional, Guillén‐Oterino, Antonio, additional, Tenorio‐Rodríguez, Daniel, additional, Velázquez, David, additional, Heger, Thierry J., additional, Sanmartín, Isabel, additional, and Lara, Enrique, additional
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- 2022
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14. Galeripora succelli González-Miguéns, Soler-Zamora, Villar-Depablo, Todorov & Lara, 2022, SP. NOV
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Galeripora succelli ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GALERIPORA SUCCELLI GONZÁLEZ- MIGUÉNS & LARA, SP. NOV. (FIG. 3) Zoobank registration: urn:lsid:zoobank.org:act: 097341BF-C706-47DD-AB62-0467FC280DCB. Holotype: MA-Algae11252. Specific diagnosis: Test diameter 180.06–200.14 µm, average of 187.77 µm (N = 19); aperture 24.37– 33.35 µm, average 29.56 µm (N = 10). Colour ranges from transparent to yellow-orange. Subhemispherical test shape, with flattened edges and dimples in the surface that gives the test a golf-ball shape. The aboral side presents no ribs or keels. Building units are covered with an organic matrix that prevents observation of the building units, giving a smooth outlook to the test. The oral side is also flat and smooth, covered with an organic matrix that prevents observation of the test building units, with a central aperture. The only pores at the base of the test are localized around the aperture, following a circular pattern and curling slightly outwards to form a small ring or lip. Intraspecific variability: The number of pores surrounding the aperture can be variable. There may be certain deformations in the test that prevent it from having a perfectly circular morphology. Diagnosis with closely related species: Galeripora succelli can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement. Galeripora succelli differs from Arcella guadarramensis by (1) its morphometric differences (see Morphometrics and morphology; Fig. 2) and it is larger than A. guadarramensis (see above); (2) a regular and marked granulation on the top of the test; and (3) the absence of pores along the edge of the arboral side of the test. Habitat: Wet Sphagnum moss, in a bog. Type locality: France, Frasne, La Tourbière (46°49’N 6°9’E). Etymology: The name is derived from the Gaulish god Succellos (-or Succellus, in its latinized form). ‘ Cellos ’ meaning striker and the prefix ‘ su ’ good, the god’s name could, therefore, be translated as ‘the Good Striker’. As god of agriculture, forests and traditional medicine, he was believed to be in charge of keeping the soil fertile. We propose this name in referring to the type locality being a fertile place with a great abundance of vegetation and surrounded by many crops., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1062, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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- 2021
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15. Galeripora balari González-Miguéns & Soler-Zamora & Villar-Depablo & Todorov & Lara 2022
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy ,Galeripora balari - Abstract
GALERIPORA BALARI GONZÁLEZ- MIGUÉNS & LARA, SP. NOV. (FIG. 4) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 8000271F-3AAD-4EBE-898B-29AB8908D5E2. Holotype: MA-Algae11256. Specific diagnosis: Test diameter: 72.95–84.20 µm, average 79.69 µm (N = 22); aperture 11.50–14.15 µm average 12.92 µm (N = 12). Colour ranges from transparent to yellow-orange. General test shape is rounded and flattened. The aboral side of the test has a small elevation at the top that gives the test a hat shape; presence of several ridges elevate the test forming a dome and flap borders; the surface does not have pores and presents a granular pattern of irregular shape. The oral side of the test is smooth, covered with an organic matrix that prevents observation of the test building units, with a central aperture. Pores are localized on the brim of the oral side and around the aperture, following a circular pattern and curling slightly outwards to form a small ring or lip. The aperture is evaginated outwards forming a short ring or lip. Intraspecific variability: The shape and number of ridges on the aboral side of the test can be variable. The number of pores around the aperture is variable. There may be certain deformations in the test that prevent it from having a perfectly circular morphology. Diagnosis with closely related species: Galeripora balari can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement (see Molecular phylogeny; Fig. 1). Differs morphologically from other Galeripora arenaria closely related species by presenting (1) morphometric differences (see Morphometrics and morphology; Fig. 2); (2) small pores along the edge of the base test; and (3) irregular and marked granulations of the top surface of the test. Habitat: Mosses overhanging from a rock, in dry gypsum terrestrial. Type locality: Spain, Castilla La Mancha, Cuenca (40°05’N 2°07’W). Etymology: The name is derived from the Irish Celtic mythology ‘ Balar ’, which means ‘the flashing one’. Balar, a Fomorian leader, was described as a giant with a large eye that causes destruction when opened. He has been inferred as an incarnation of drought, plague and burning sun. We propose this name because the morphology of this species is similar to that of an eye, and because the type locality is a dry area.
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- 2021
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16. Galeripora galeriformis González-Miguéns, Soler-Zamora, Villar-Depablo, Todorov & Lara, 2022, SP. NOV
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Galeripora galeriformis ,Taxonomy - Abstract
GALERIPORA GALERIFORMIS GONZÁLEZ- MIGUÉNS, SOLER- ZAMORA, VILLAR- DEPABLO, TODOROV & LARA, SP. NOV. (FIG. 4) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 09628A92-07D4-4DA7-BF68-A803B3A9D5D5. Holotype: MA-Algae11253. Specific diagnosis: Test diameter: 71.65–74.95 µm, average 73.20 µm (N = 4); aperture 11.15 to 12.10 µm average 11.50 µm (N = 4). Colour ranges from transparent to yellow-orange. General test shape is rounded and flattened. The aboral side of the test has a small elevation at the top that gives the test a helmet shape; the surface does not have pores and presents a granular pattern of irregular shape. Large ridges cross the aboral side of the test. The oral side of the test is smooth, covered with an organic matrix that prevents the observation of test building units, with a central aperture. Pores are localized on the brim of the oral side and around the aperture, following a circular pattern. The aperture is invaginated outwards forming a short ring or lip. Intraspecific variability: The shape and number of ridges on the aboral side of the test can be variable. The number of pores around the aperture is variable. There may be certain deformations in the test that prevent it from having a perfectly circular morphology. Diagnosis with closely related species: Galeripora galeriformis can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement (see Molecular phylogeny; Fig. 1). Differs morphologically from other species closely related to Galeripora arenaria by presenting (1) morphometric differences (see Morphometrics and morphology; Fig. 2), with a smaller test and aperture diameters than all species presented here; (2) pores along the edge of the base test; (3) presence of a dome in the top part of the test; and (4) irregular granulations of the top surface of the test. Habitat: Moss on dry gypsum; terrestrial. Type locality: Spain, Madrid, Rivas-Vaciamadrid, near Laguna del Campillo (40°19’N 3°30’W). Etymology: The name is derived from the Latin galerus, helmet, and forma, shape. We propose this name because a Roman helmet is round and wide with a flat brim, similar to the test of this species., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on pages 1062-1063, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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- 2021
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17. Arcella guadarramensis González-Miguéns, Soler-Zamora, Villar-Depablo, Todorov & Lara, 2022, SP. NOV
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Arcella guadarramensis ,Arcellinida ,Lobosa ,Arcella ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
ARCELLA GUADARRAMENSIS GONZÁLEZ- MIGUÉNS & LARA, SP. NOV. (FIG. 8) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 57A3452C-3C46-44F2-A5C9-48496E34BB57. Holotype: MA-Algae11251. Specific diagnosis: Test diameter: clade L: 114.60– 125.90 µm, average 120.30 µm (N = 6); aperture 19.60– 30.00 µm, average 23.63 µm. clade M 141.50–149.95 µm, average 146.06 µm (N = 4); aperture 31.25–34.50 µm, average 33.08 µm. Besides a difference in size, both clades have an identical morphology. Colour ranges from transparent to yellow-orange. Subhemispherical test shape, with flattened edges and dimples in the surface that gives the test a golf ball shape. No ribs or keels on the aboral side. Hexagonal building units are visible, which gives the test a rough appearance; little pores can be seen at the vertices. Building units can also be appreciated at the oral side of the test, with pores at the vertices and a central aperture. The aperture is invaginated outwards forming a short ring or lip. Intraspecific variability: The building units may vary slightly in shape. Some building units may be collapsed, giving a rough surface. There may be certain deformations in the test that prevent it from having a perfectly circular morphology. Diagnosis with closely related species: Arcella guadarramensis can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement. Arcella guadarramensis differs morphologically from similar-looking G. succelli by (1) its morphometric differences with G. succelli (see Morphometrics and morphology; Fig. 2), both, clade N and O, are notably smaller than G. succelli and (2) the rough outlook of the test. Habitat: Wet Sphagnum moss, in a fen. Type locality: Spain, Madrid, Puerto de Canencia (40°52’N 3°45’W). Etymology: The name is derived from River Guadarrama, a river with a name of Arabic roots: wadi, river, and ar-rama, sandy. We propose this name as a reference to the type locality in ‘Sierra de Guadarrama’, a mountain range named after this river. We provide a key (Supporting Information, Table S3) and a new figure (Supporting Information, Fig. S3) to facilitate the identification of the new species., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on pages 1065-1066, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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18. Galeripora González-Miguéns, Soler-Zamora, Villar-Depablo, Todorov & Lara, 2022, GEN. NOV
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GENUS GALERIPORA GONZÁLEZ- MIGUÉNS, SOLER- ZAMORA, VILLAR- DEPABLO, TODOROV & LARA GEN. NOV. Zoobank registration: urn:lsid:zoobank.org:act: 73C0B8C8-FFA4-48CC-8AE5-B0266D983995. Type species: Galeripora sitiens González-Miguéns, Soler-Zamora, Villar-dePablo, Todorov & Lara Description: Tests built exclusively with proteinaceous organic material. Shape more or less campanulate, with a central and circular aperture and radial symmetry. The aperture is surrounded by pores. The ultrastructure of the test is composed of hexagonal units that are at least partially covered with an organic matrix layer. Commonly, two or more nuclei, situated at opposite sides of the cytoplasm. Derivatio nominis: The name is derived from the Latin galerus, helmet and ‘ -por ī’, translated as ‘helmet-pores’. This name suggests the general shape of the theca that forms the members of this group, and the pores surrounding the opening, one of the synapomorphies of this genus. Included taxa: Galeripora arenaria, G. balari, G. bathystoma, G. bufonipellita, G. catinus, G. discoides, G. galeriformis, G. naiadis, G. polypora, G. sitiens and G. succelli., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1062, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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19. Arcella Ehrenberg 1830
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Arcellinida ,Lobosa ,Arcella ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GENUS ARCELLA EHRENBERG, 1830 Type species: Arcella vulgaris Ehrenberg, 1830. Included taxa: (Supporting Information, Appendix S1)., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1065, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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20. bathystoma
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Biodiversity ,Protozoa ,Taxonomy - Abstract
GALERIPORA BATHYSTOMA (DEFLANDRE, 1928) COMB. NOV. (FIG. 5) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 71B87508-D89E-4DD6-88DD-BA74DBDB3303., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1065, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266, {"references":["Deflandre G. 1928. Le genre Arcella Ehrenberg. Archiv fur Protistenkunde 64: 152 - 287."]}
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21. Galeripora arenaria
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Arcellidae ,Galeripora arenaria ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GALERIPORA ARENARIA(GREEF, 1866)COMB. NOV. (FIG. 4) Arcella aureola Maggi, 1888. Arcella microstoma Penard, 1890. Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: C1404AAE-CD5B-4825-9B47-F746BBF4E007., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1065, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266, {"references":["Greef R. 1866. Ueber einige in der Erde lebende Amoben und andere Rhizopoden. Archiv fur Mikroskopische Anatomie 2: 299 - 331.","Maggi, L. 1888. Sur les Protozoaires vivant sur les mousses des plantes. Archivio Italiano di Biologia 10: 184 - 189.","Penard E. 1890. Etudes sur les Rhizopodes d'eau douce. Memoires dela Societe de physique et d'histoire naturelle de Geneve 31: 1 - 230."]}
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22. Netzelia lobostoma
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Arcellinida ,Lobosa ,Netzelia lobostoma ,Biodiversity ,Protozoa ,Amoebozoa ,Netzelia ,Taxonomy ,Lesquereusiidae - Abstract
NETZELIA LOBOSTOMA(LEIDY, 1874)COMB. NOV. Difflugia proteiformis Lamarck, 1816: 95. Cucurbitella tricuspis – Carter, 1856: 221; Medioli et al., 1987. Difflugia tricuspis Carter, 1856: 221. Difflugia lobostoma Leidy, 1874: 79. Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 0AD69577-89AD-42E8-9C40-03A68C73EFD4., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1061, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266, {"references":["Leidy J. 1874. Notice of some new fresh-water rhizopods. Proceedings of the Academy of Natural Sciences of Philadelphia 3 rd Series 26: 77 - 79.","Carter HJ. 1856. Notes on the freshwater Infusoria of the island of Bombay. Annals and Magazine of Natural History Ser. 2 18: 221 - 249.","Medioli FS, Scott DB, Abbott BH. 1987. A case study of protozoan intraclonal variability: taxonomic implications. Journal of Foraminiferal Research 17: 28 - 47."]}
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23. Galeripora bufonipellita González-Miguéns, Soler-Zamora, Villar-Depablo, Todorov & Lara, 2022, SP. NOV
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora bufonipellita ,Galeripora ,Arcellinida ,Lobosa ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GALERIPORA BUFONIPELLITA GONZÁLEZ- MIGUÉNS & LARA, SP. NOV. (FIG. 4) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 8262B0F0-8E1B-48E4-902D-DB867DC0D5BE. Holotype: MA-Algae11254. Specific diagnosis: Test diameter: 89.05–92.95 µm, average 90.35 µm (N = 8); aperture 13.45–17.85 µm, average 14 µm (N = 4). Colour ranges from transparent to yellow-orange. General test shape is rounded and flattened. The aboral side of the test has several ridges that elevate the test forming a dome and flap borders; the surface does not have pores and presents a granular pattern of regular shape. The oral side of the test is smooth, covered with an organic matrix that prevents the observation of test building units, with a central aperture. Pores are localized only around the aperture, following a circular pattern and curling slightly outwards to form a small ring or lip. The aperture is invaginated outwards forming a short ring or lip. Intraspecific variability: The shape and number of ridges on the aboral side of the test can be variable. The number of pores around the aperture is variable. There may be certain deformations in the test that prevent it from having a perfectly circular morphology. Diagnosis with closely related species: Galeripora bufonipellita can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement. Differs morphologically from Galeripora arenaria closely related species by (1) morphometric differences (see Morphometrics and morphology; Fig. 2); (2) the absence of pores along the edge of the aboral side of the test; and (3) a regular and marked granulation in the top surface of the test. Habitat: Mosses on the edge of a lake, partially (and temporally) submerged, growing on granitic rocks. Type locality: Spain, Madrid, Rascafría (40°51’N 3°56’W) and (40º52’N 3º52’W) and San Lorenzo de El Escorial (40°34’N 4°09’W). Etymology: The name is derived from the Latin bufo, toad and pellis, animal skin. We propose this name because of the ornamentation of the aboral side of the test, which is reminiscent of the warty skin of a toad., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1063, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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24. Galeripora naiadis González-Miguéns, Soler-Zamora, Villar-Depablo, Todorov & Lara, 2022, SP. NOV
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Galeripora naiadis ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GALERIPORA NAIADIS GONZÁLEZ- MIGUÉNS, SOLER- ZAMORA, VILLAR- DEPABLO, TODOROV & LARA, SP. NOV. (FIG. 5) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: A6005873-8045-40E2-B0E1-DE073A2EC66B. Specific diagnosis: Test diameter 139.20–153.80 µm, average of 146.78 µm (N = 21); aperture 48.70– 59.10 µm, average 53.76 µm (N = 21). Colour ranges from transparent to yellow-orange. The test has a discoid, flattened shape. The edges of the test are somehow curved, giving the whole test a concave outlook, like a bowl. The aboral side of the test is flat. Building units can be partially covered with a proteinaceous matrix preventing the observation of these units. When this proteinaceous matrix is absent, small pores can be observed at the vertices of the building units, which are then located on both the aboral and oral sides. The oral side presents an aperture invaginated outwards forming a short ring or lip surrounded by many small pores. Intraspecific variability: The number of pores at the base of the test and the degree of coverage by the organic matrix can be variable. There may be certain deformations in the test that prevent it from having a perfectly circular morphology. Differences: Galeripora naiadis can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement. Differs morphologically from other species closely related to Galeripora polypora by presenting (1) morphometric differences (see Morphometrics and morphology; Fig. 2) and (2) by its concave outlook. Habitat: Submerged vegetation, Ceratophyllum submersum, in an artificial lake. Type locality: Bulgaria: Sofia, Sofia Southern Park (42°39’N 23°18’E). Etymology: The name is derived from the Greek Ναϊάδες, the Naiads, who were female spirits or nymphs who ruled the rivers in Ancient Greek mythology. We propose this name to refer to the ecology of this species – pristine freshwater environments., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on pages 1064-1065, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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25. Galeripora polypora
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Galeripora polypora ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GALERIPORA POLYPORA (PENARD, 1890) COMB. NOV. (FIG. 5) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 77A12E43-4702-4BBD-B26A-F1E2A093955F., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1065, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266, {"references":["Penard E. 1890. Etudes sur les Rhizopodes d'eau douce. Memoires dela Societe de physique et d'histoire naturelle de Geneve 31: 1 - 230."]}
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26. Galeripora sitiens GONZÁLEZ- MIGUÉNS, SOLER- ZAMORA, VILLAR- DEPABLO, TODOROV & LARA, SP. NOV
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora sitiens ,Galeripora ,Arcellinida ,Lobosa ,Arcellidae ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
GALERIPORA SITIENS GONZÁLEZ- MIGUÉNS, SOLER- ZAMORA, VILLAR- DEPABLO, TODOROV & LARA, SP. NOV. (FIG. 4) Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 02BD3509-F96A-4EA0-8131-03DC5DD5BA6B. Holotype: MA-Algae11255. Specific diagnosis: Test diameter: 86.50–92.65 µm, average 88.89 µm (N = 8); aperture 16.20–16.85 µm, average 16.60 µm (N = 3). Colour ranges from transparent to yellow-orange. General test shape is rounded and flattened. The aboral side of the test has several ridges that elevate the test forming a dome and flap borders; the surface does not have pores and presents a granular pattern of regular shape. The oral side of the test is smooth, covered with an organic matrix that prevents observation of the test building units, with a central aperture. Pores are localized only around the aperture, following a circular pattern. The aperture is invaginated outwards forming a short ring or lip. Intraspecific variability: The shape and number of ridges on the aboral side of the test can be variable. The number of pores around the aperture is variable. There may be certain deformations in the test that prevent it from having a perfectly circular morphology. Diagnosis with closely related species: Galeripora sitiens can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement. Differs morphologically from Galeripora arenaria and closely related species by (1) morphometric differences (see Morphometrics and morphology; (Fig. 2); (2) the absence of pores along the edge of the aboral side of the test; and (3) a regular granulation of the top surface of the test. Habitat: Moss on dry slate and quartzite terrestrial. Type locality: Spain, Castilla La Mancha, Almonacid de Toledo (39°44’N 3°51’W). Etymology: The name is derived from the Latin sitiens, dry or thirsty. We propose this name because the habitat of this species is characterized by prolonged drought with episodic rainfall., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on pages 1063-1064, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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27. Galeripora catinus
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Galeripora ,Arcellinida ,Lobosa ,Arcellidae ,Biodiversity ,Protozoa ,Galeripora catinus ,Amoebozoa ,Taxonomy - Abstract
GALERIPORA CATINUS (PENARD, 1890) COMB. NOV. (FIG. 6) Arcella catinus var. australis Playfair, 1918. Arcella vulgaris var. compressa Cash, 1905. Z o o b a n k r e g i s t r a t i o n: u r n: l s i d: z o o b a n k. org:act: 4E606BBB-DB09-4125-8FE8-579064E97E08. GALERIPORA DISCOIDES (EHRENBERG, 1843) COMB., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1065, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266, {"references":["Penard E. 1890. Etudes sur les Rhizopodes d'eau douce. Memoires dela Societe de physique et d'histoire naturelle de Geneve 31: 1 - 230.","Playfair GI. 1918. Rhhizopods of Sydney and Lismore. Proceedings ofthe Linnean Society of New South Wales 42: 633 - 675."]}
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28. Sphaerothecina KOSAKYAN ET AL. 2016
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
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Arcellinida ,Lobosa ,Biodiversity ,Protozoa ,Amoebozoa ,Taxonomy - Abstract
INFRAORDER SPHAEROTHECINA KOSAKYAN ET AL., 2016 Updated diagnosis: Tests have a central aperture, are rounded and have a radial symmetry in crosssection. Most have the capacity to build their own test without foreign material, possibly with self-secreted components. Type family: Netzeliidae. Included taxa: (Supporting Information, Appendix S1)., Published as part of González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho & Lara, Enrique, 2022, Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology, pp. 1044-1071 in Zoological Journal of the Linnean Society 194 (4) on page 1060, DOI: 10.1093/zoolinnean/zlab074, http://zenodo.org/record/6461266
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29. Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology
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González-Miguéns, Rubén, primary, Soler-Zamora, Carmen, additional, Villar-Depablo, Mar, additional, Todorov, Milcho, additional, and Lara, Enrique, additional
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30. Arcellinida testate amoebae as climate miner's canaries in Southern Spain
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Soler-Zamora, Carmen, primary, González-Miguéns, Rubén, additional, Guillén-Oterino, Antonio, additional, and Lara, Enrique, additional
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31. Arcellinida testate amoebae as climate miner's canaries in Southern Spain
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Soler-Zamora, Carmen, González-Miguéns, Rubén, Guillén-Oterino, Antonio, Lara, Enrique, Soler-Zamora, Carmen, González-Miguéns, Rubén, Guillén-Oterino, Antonio, and Lara, Enrique
- Abstract
Southern Spain is currently under threat of desertification as a consequence of global climate change, which pressures on fragile ecosystems such as caves. The organisms living in these extremely stable environments are particularly sensitive and prone to extinction, therefore they can be used as bioindicators for climate change. Cyanobacterial mats form peculiar and vulnerable micro-ecosystems at the entrance of caves and house a diversity of protists. Amongst them, Arcellinida testate amoebae have been traditionally used as bioindicators for environmental quality, notably because their narrow ecological tolerance and their key ecological position as top predators of the microbial foodwebs. We report here two new species of Arcellinida found in the cyanobacterial mats of cave Hundidero, in Sierra de Grazalema, Malaga province, whose traits suggest a narrow tolerance for changes in humidity. We provide a formal description for Difflugia alhadiqa sp. nov. and Heleopera baetica sp. nov. based on morphometrics and 18S rRNA gene data, and propose using the presence of these species to indicate the good health of the cyanobacterial mats, like miner's canaries for local climate.
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32. A taxonomic revision of the genus Cyphoderia Schlumberger,1845 (Euglyphida)
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González-Miguéns, Rubén, Lara Pandi, Enrique Miguel, and Useros López, Fernando
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- 2020
33. Speciation patterns in the Forficula auricularia species complex: cryptic and not so cryptic taxa across the western Palaearctic region
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Ministerio de Ciencia, Innovación y Universidades (España), European Commission, González-Miguéns, Rubén, Muñoz-Nozal, Eva, Jiménez-Ruiz, Yolanda, Mas-Peinado, Paloma, Ghanavi, Hamid Reza, García-París, Mario, Ministerio de Ciencia, Innovación y Universidades (España), European Commission, González-Miguéns, Rubén, Muñoz-Nozal, Eva, Jiménez-Ruiz, Yolanda, Mas-Peinado, Paloma, Ghanavi, Hamid Reza, and García-París, Mario
- Abstract
Forficula auricularia (the European earwig) is possibly a complex of cryptic species. To test this hypothesis, we performed: (1) a phylogeographic study based on fragments of the mitochondrial COI and the nuclear ITS2 markers on a wide geographic sampling, (2) morphometric analyses of lineages present in Spain and (3) niche overlap analyses. We recovered five reciprocally monophyletic ancient phylogroups with unique historical patterns of distribution, climatic niches and diversification. External morphology was conserved and not correlated with speciation events, except in one case. Phylogenetic placement of the morphologically distinct taxon renders F. auricularia paraphyletic. Based on the congruence of the phylogenetic units defined by mtDNA and nuclear sequence data, we conclude that phylogroups have their own historical and future evolutionary trajectory and represent independent taxonomic units. Forficula auricularia is a complex of at least four species: the morphologically diagnosable Forficula aeolicaGonzález-Miguéns & García-París sp. nov., and the cryptic taxa: Forficula mediterranea González-Miguéns & García-París sp. nov., Forficula dentataFabricius, 1775 stat. nov. and Forficula auriculariaLinnaeus, 1758 s.s. New synonymies of F. dentata are also proposed.
- Published
- 2020
34. Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology.
- Author
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González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho, and Lara, Enrique
- Subjects
- *
AMOEBA , *ENVIRONMENTAL indicators , *ENVIRONMENTAL quality , *MORPHOLOGY - Abstract
Protists are probably the most species-rich eukaryotes, yet their systematics are inaccurate, leading to an underestimation of their actual diversity. Arcellinida (= lobose testate amoebae) are amoebozoans that build a test (a hard shell) whose shape and composition are taxonomically informative. One of the most successful groups is Arcellidae, a family found worldwide in many freshwater and terrestrial environments where they are indicators of environmental quality. However, the systematics of the family is based on works published nearly a century ago. We re-evaluated the systematics based on single-cell barcoding, morphological and ecological data. Overall, test shape appears to be more related to environmental characteristics than to the species' phylogenetic position. We show several convergences in organisms with similar ecology, some traditionally described species being paraphyletic. Based on conservative traits, we review the synapomorphies of the infraorder Sphaerothecina, compile a list of synonyms and describe a new genus Galeripora , with five new combinations. Seven new species: Arcella guadarramensis sp. nov. Galeripora balari sp. nov. Galeripora bufonipellita sp. nov. Galeripora galeriformis sp. nov. Galeripora naiadis sp. nov. Galeripora sitiens sp. nov. and Galeripora succelli sp. nov. are also described here. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
35. Speciation patterns in the Forficula auricularia species complex: cryptic and not so cryptic taxa across the western Palaearctic region
- Author
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González-Miguéns, Rubén, primary, Muñoz-Nozal, Eva, additional, Jiménez-Ruiz, Yolanda, additional, Mas-Peinado, Paloma, additional, Ghanavi, Hamid R, additional, and García-París, Mario, additional
- Published
- 2020
- Full Text
- View/download PDF
36. Presencia de Euborellia annulipes (Lucas, 1847) (Dermaptera, Anisolabididae) en áreas costeras del atlántico ibérico (Galicia, España).
- Author
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GONZÁLEZ-MIGUÉNS, RUBÉN
- Published
- 2017
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