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Odontogenic cysts are frequently encountered in clinical practice. However, residual cysts, a specific type of inflammatory odontogenic cyst, are relatively rare. These cysts may slowly expand over time, damaging surrounding soft tissues and bone, typically without posing a threat to life. We report the case of a 67-year-old man with liver failure and a cystic tumor in his right maxilla that had invaded his oral cavity and cheek, causing nasal obstruction and severe bleeding following an incisional biopsy. A computed tomography (CT) scan of the mass was nonspecific, and an initial histopathological analysis of the tissues was inconclusive due to chronic inflammation and hemorrhagic alterations, complicating the diagnostic pathway. The suspicion of a potentially aggressive malignant neoplasm and the need for immediate intervention due to bleeding necessitated a tracheotomy, tumor removal, percutaneous endoscopic gastrostomy, and transfusions of red blood cells. A subsequent histopathological examination revealed features indicative of a residual cyst. The entire lesion was excised through functional endoscopic sinus surgery. The surgical treatment was performed safely and effectively. Follow-up CT confirmed complete removal of the lesion. This case highlights a rare yet possible complication of odontogenic cysts and underscores the necessity of early diagnosis and comprehensive prophylaxis to prevent severe complications.

Broadly, understood additive manufacturing techniques expand the spectrum of production of machine parts that are used in various types of devices. However, the requirements to ensure dimensional and shape accuracy require the determination of appropriate material allowances or clearances to enable correct assembly. For the above reasons, the study presents an analysis of the impact of the assumed geometry modifications on the possibility of assembling a splined connection manufactured using selected AM techniques. The work focused on the analysis of changes in geometry resulting from the technology used. Using the Atos II Triple Scan optical measurement system and Gom Inspect software, the possibility of installation was determined for four variants of the splined shaft connection geometry, resulting from the technologies used.

Warthin tumors (WTs) are the second most common salivary gland tumors, most commonly found in the tail of the parotid gland. The lymphoid components of a WT may also serve as a site for tumor-to-tumor metastasis. This report describes the metastasis of cutaneous squamous cell carcinoma (cSCC) from the preauricular region to a parotid WT. A 68-year-old male patient was admitted to the Department of Cranio-Maxillofacial Surgery of the Jagiellonian University in Cracow, Poland, with a two-year history of a growing, painless skin tumor of the right preauricular region. The patient was eligible for surgical treatment with elective neck dissection at the Va, III, II, I levels with parotidectomy and the excision of the cSCC. In the histopathological examination of the components of the surgical specimen beside the primary cutaneous squamous cell carcinoma (cSCC), a parotid WT was found; in the majority, it was occupied and destroyed by the metastatic cSCC and radically removed. After a tumor board consultation, due to the advanced stage (pT3N2b LVI2 PNI0 R0), with three intraparotid lymph node metastases and LVI2, the patient was authorized for postoperative radiotherapy. In conclusion, tumor-to-tumor metastasis is an extremely rare entity. Surgery is the standard of care for both WTs and head and neck cSCC. In most cases, metastasis into the parotid gland can be successfully treated with superficial parotidectomy with facial nerve preservation. Dissemination into the parotid gland requires elective neck dissection, as well as adjuvant treatment.

Conclusions: The SGF is effective for achieving successful reconstruction of small- and medium-sized defects in the lower gingiva and floor of the mouth. The complication rate is relatively low.

This paper presents a new method for forming flanges in hollow parts. The process consists of an extrusion with two dies that move in an opposite direction to that of the punches. This particular kinematics of the tools makes it possible to form two flanges simultaneously in a single tool pass. The proposed method was investigated using a tube made of steel 42CrMo4. It was assumed that the extrusion would be conducted as a cold forming process at ambient temperature. Different diameters and heights of the impression made in the top and bottom dies were used. It was demonstrated that the main failure mode of the proposed technique was an unintended increase in the inside the diameter of the workpiece in the flange zone. The results showed that the above parameters had a key impact on the achievable maximum flange diameters and heights.

(1) Background: Dermoid cysts occurring in the sublingual space are uncommon, typically manifesting as painless, gradually enlarging masses, usually not exceeding 3 cm in diameter. These cysts can resemble various conditions due to their clinical presentation, with a relatively low occurrence rate in the oral cavity, accounting for about 1.6% of all dermoid cysts. (2) Methods: We present the case of a 17-year-old female with a giant dermoid cyst involving the submental, sublingual, and lingual areas, undiagnosed for several years. Diagnosis was achieved through MRI and fine-needle aspiration, leading to the decision for surgical removal through a cervical approach. (3) Results: The healing process was uneventful. From the first day post-surgery, the patient began myofunctional therapy, successfully regaining proper tongue functions. Throughout a 24-month follow-up, the patient remained symptom-free. (4) Conclusions: A cervical approach can successfully treat giant oral dermoid cysts involving submental, sublingual, and lingual spaces. Tongue function can be successfully regained through myofunctional therapy after surgical treatment.

Theoretical background: The article will help to answer the question what factors contribute to the emergence of new companies in medical, agri-food and creative sectors. Moreover, the aim of the article is to examine to what extent the budget policy of communes determines the creation of new enterprises in each of the analysed sectors separately. Purpose of the article: The aim of the article is to examine the factors affecting new firms formation in medical, agri-food and creative sectors in Poland, as well as the impact of budget policy of local government units at commune level on entrepreneurship in chosen sectors. Research methods: The study was conducted by means of a panel econometric model. In particular, the study was based on FEM estimation modelling based on 2,477 communes in the years 2010–2021. Main findings: The conducted research confirmed that different factors affect new firms formation in studied sectors. The research proved that budget policy of communes influences new firms formation. Moreover, the study confirmed that individual elements of the local budget policy have a different impact on each economic activity. Thus, local budget policy should be profiled in terms of type of activity supported.

Background: Diagnostic errors constitute one of the reasons for the improper and often delayed treatment of mandibular fractures. The aim of this study was to present a series of cases involving undiagnosed concomitant secondary fractures in the mandibular body during preoperative diagnostics. Additionally, this study aimed to describe the “air sign” as an indirect indicator of a mandibular body fracture. Methods: A retrospective analysis of CT/CBCT scans conducted before surgery was performed on patients misdiagnosed with a mandibular body fracture within a one-year period. Results: Among the 75 patients who underwent surgical treatment for mandibular fractures, mandibular body fractures were missed in 3 cases (4%) before surgery. The analysis of CT/CBCT before surgery revealed the presence of an air collection, termed the “air sign”, in the soft tissue adjacent to each misdiagnosed fracture of the mandibular body. Conclusions: The “air sign” in a CT/CBCT scan may serve as an additional indirect indication of a fracture in the mandibular body. Its presence should prompt the surgeon to conduct a more thorough clinical examination of the patient under general anesthesia after completing the ORIF procedure in order to rule-out additional fractures.

(1) Background: “Eagle Syndrome”, also known as “stylalgia” or a “stylohyoid chain anomaly”, typically manifests with ipsilateral orofacial pain, a foreign body sensation in the throat, and ear-related symptoms. Despite these common presentations, its potential association with carotid artery dissection is not widely acknowledged. (2) Methods: This article presents an extremely rare case of a patient diagnosed with an ischemic stroke in the left hemisphere, followed by the dissection of the left internal carotid artery, initially with an unidentified cause. Subsequent examinations revealed elongated left styloid processes directly compressing the dissected artery. (3) Results: After initial treatment involving pharmacological and mechanical thrombectomy, styloidectomy restored blood flow to the internal carotid artery. The patient remained symptom-free during a 12-month follow-up. (4) Conclusions: This case emphasizes the importance of considering anatomical variations within the stylohyoid chain when assessing young individuals with neurological symptoms. Furthermore, it underscores the potential benefits of early surgical intervention in reducing the morbidity and mortality associated with this condition.

Abstract Mutations in the chromodomain helicase DNA binding protein 2 (CHD2) gene are associated with neurodevelopmental disorders. However, mechanisms by which CHD2 regulates human brain development remain largely uncharacterized. Here, we used a human embryonic stem cell model of cortical interneuron (hcIN) development to elucidate its roles in this process. We identified genome-wide CHD2 binding profiles during hcIN differentiation, defining direct CHD2 targets related to neurogenesis in hcIN progenitors and to neuronal function in hcINs. CHD2 bound sites were frequently coenriched with histone H3 lysine 27 acetylation (H3K27ac) and associated with high gene expression, indicating roles for CHD2 in promoting gene expression during hcIN development. Binding sites for different classes of transcription factors were enriched at CHD2 bound regions during differentiation, suggesting transcription factors that may cooperatively regulate stage-specific gene expression with CHD2. We also demonstrated that CHD2 haploinsufficiency altered CHD2 and H3K27ac coenrichment on chromatin and expression of associated genes, decreasing acetylation and expression of cell cycle genes while increasing acetylation and expression of neuronal genes, to cause precocious differentiation. Together, these data describe CHD2 direct targets and mechanisms by which CHD2 prevents precocious hcIN differentiation, which are likely to be disrupted by pathogenic CHD2 mutation to cause neurodevelopmental disorders.

Introduction Ranulas are divided into oral (OR) and plunging (PR) and comprise the most common pathology of the sublingual gland. This study presents a case series of patients operated due to OR and PR within different type of modalities in a 1-year period. Objective The aim of this study is to determine the optimal surgical treatment of ranulas based on our results as well as in the literature review. Methods The medical charts of 7 patients with sublingual gland ranulas treated in 2020 were reviewed. Results The median age of the patients was 19. Three patients with OR were treated by marsupialization, micromarsupialization, and sublingual gland excision. Four patients with PR were operated via cervical approach in three cases and intraoral approach in one case. No recurrence was observed in 14 months of follow-up, on average. Conclusion Micromarsupialization should be consider as the primary treatment for OR. In case of recurrent OR and primary or recurrent PR, the best results might be obtained by radical excision of the sublingual gland, which can be performed without resection of the ranula sac with the intraoral approach.

Introduction Eosinophilic granulomatosis with polyangiitis (eGPA) is a rare type of vasculitis. We described the case of the patient who was admitted to our Department due to haemoptysis, in whom the model-based clinical reasoning and quick diagnostic process led to the diagnosis and referral of the patient to specialists dealing with the treatment of this disease. We wanted to emphasize the fact that the patient developed symptoms of the disease many years before diagnosis. However, these clinical problems were treated individually. A broader reflection of the patient’s symptoms may have led to earlier diagnosis and implementation of adequate treatment Objective The aim of the study is to underline the importance of clinical reasoning on the example of eGPA model diagnostics. The combination of several clinical findings led to the correct diagnosis, which enabled the patient to obtain treatment and improved further prognosis.

Abstract Background Neurodevelopmental disorders increase brain tumor risk, suggesting that normal brain development may have protective properties. Mutations in epigenetic regulators are common in pediatric brain tumors, highlighting a potentially central role for disrupted epigenetic regulation of normal brain development in tumorigenesis. For example, lysine 27 to methionine mutation (H3K27M) in the H3F3A gene occurs frequently in Diffuse Intrinsic Pontine Gliomas (DIPGs), the most aggressive pediatric glioma. As H3K27M mutation is necessary but insufficient to cause DIPGs, it is accompanied by additional mutations in tumors. However, how H3K27M alone increases vulnerability to DIPG tumorigenesis remains unclear. Results Here, we used human embryonic stem cell models with this mutation, in the absence of other DIPG contributory mutations, to investigate how H3K27M alters cellular proliferation and differentiation. We found that H3K27M increased stem cell proliferation and stem cell properties. It interfered with differentiation, promoting anomalous mesodermal and ectodermal gene expression during both multi-lineage and germ layer-specific cell specification, and blocking normal differentiation into neuroectoderm. H3K27M mutant clones exhibited transcriptomic diversity relative to the more homogeneous wildtype population, suggesting reduced fidelity of gene regulation, with aberrant expression of genes involved in stem cell regulation, differentiation, and tumorigenesis. These phenomena were associated with global loss of H3K27me3 and concordant loss of DNA methylation at specific genes in H3K27M-expressing cells. Conclusions Together, these data suggest that H3K27M mutation disrupts normal differentiation, maintaining a partially differentiated state with elevated clonogenicity during early development. This disrupted response to early developmental cues could promote tissue properties that enable acquisition of additional mutations that cooperate with H3K27M mutation in genesis of DMG/DIPG. Therefore, this work demonstrates for the first time that H3K27M mutation confers vulnerability to gliomagenesis through persistent clonogenicity and aberrant differentiation and defines associated alterations of histone and DNA methylation.

The aim of this study was to compare treatment modalities, pathological and clinical characteristics, and outcomes in patients with metastasis in a parotid gland. The medical records of 34 patients who received treatment for metastasis in the parotid gland over a twenty-year period were evaluated. Patients with head and neck cutaneous squamous cell carcinoma (HNcSCC) metastasis were retrospectively reclassified using the P/N and N1S3 staging system. Patients with neck metastasis showed a significantly poorer prognosis (P = 0.025). Univariate analysis also revealed that extent of parotidectomy and type of neck dissection did not influence recurrence free survival (RFS) and overall survival (OS). When comparing the usefulness of the P/N and S1N3 staging systems, a positive correlation was observed between the P stage and the N1S3 stage in both RFS and OS. The extent of parotidectomy and concomitant neck dissection is still under discussion. Total parotidectomy and modified radical neck dissection did not improve RFS and OS. N1S3 is a less complex classification and possesses a higher predictive value when compared to the P/N staging system. [ABSTRACT FROM AUTHOR]

The present work aims at the analysis of the factors that influence animal size and fur quality in blue fox (Alopex lagopus L). The data proceeds from a breeding farm situated in central Poland and concerns three years. The analysis of variance indicates statistically significant effect of the year of birth on animal size, fur quality and colour purity. The birth term had no statistical influence on the analysed traits. Animal sex had significant influence on the following traits: animal size, fur quality and total score. The interactions between year and animal sex, year and date of birth, as well as animal sex and the date of birth did not have statistically significant effect on the measured traits. Estimated phenotypic correlations in the analysed herd ranged from negative to positive. The highest correlations occurred between fur quality and total score (0.800), while the lowest between animal size and fur quality (-0.600). The foxes from the analysed breeding farm were characterised by good parameters of fur and are good breeding material.

The work aimed at the analysis of the influence of colour type on chinchilla utilitarian traits. Research concerns animal size and fur quality traits. The data for the analysis was collected from the breeding farm „Raba” in Myślenice (southern Poland). The data concerns standard and Polish beige chinchillas and covers 3 years. The analysis included the following factors: year of evaluation, animal sex, colour type and interactions of these factors. The analysis of variance proved statistically significant influence of the year of evaluation, colour type and animal sex on evaluated traits. The year of observation influenced significantly animal weight, fur colour purity, colour type, fur’s paunch part and total number of scores. Colour type influenced significantly animal weight and fur colour purity. Animal sex influenced significantly animal weight, animal size, animal build, fur quality, fur colour purity and total number of scores. Arithmetic means of studied traits during 3 years reached various levels, depending on colour type and animal sex; e.g. animal weight (in grams) in chinchilla ranged from about 560 g to 620 g. In what concerns total number of scores (in points), e.g. males of standard colour types were evaluated higher than females of beige colour type, while females of beige colour type obtained higher notes when compared with females of standard colour type. Variability coefficients, depending on traits, varied from 7.15% to 26.17%. It can prove that chinchillas which obtained very high notes in some traits had lower notes in other traits and consequently total number of scores was equalized. Phenotypic correlations were also estimated and ranged from -0.089 to 0.721.

The work aimed at the analysis of the factors that influence conformation traits, included animal size and fur quality traits in four colour types of mink: standard, pastel, platinum and white Hedlunda. The data concerns the evaluation of animal conformation traits in the period of three years. The analysis of variance of particular traits indicates statistically significant effect of the year of birth, colour type and animal sex on the majority of analysed traits. Higher means of license evaluation were obtained by males in majority of the traits. Statistic analysis of body weight showed that the highest body weight characterized males of platinum and white Hedlunda colour types. Minks of standard and pastel colour types were characterised by lower body weight. The mean body weight of males was 2581.17g and of females 1401.42g (there is a clear sexual dimorphism in minks). Minks of white Hedlunda colour type were characterised by the highest means of colour purity, both males and females. Other colour types obtained lower means. The best fur quality characterised platinum minks. Variability of traits, measured by variability coefficient, had the highest values in animal weight (in grams) and ranged from 6.0 to 32.0%. Variability of total number of scores ranged from 2.00 to 8.20%. Positive phenotypic correlations were the highest between body size (in points) and total number of scores (0.676), while the lowest were obtained between body size (in points) and fur quality (–0.178).

Abstract Background Salvage surgery with reconstruction of the second and next primary tongue cancer remains difficult, especially after earlier neck dissection and radiotherapy. In the current report, we describe the feasibility of the extended, double-pedicled facial artery musculomucosal (dpFAMM) flap in the reconstruction of the patient with second primary tongue squamous cell carcinoma, after facial vessel ligation and radiotherapy. Case presentation An 81-year-old female patient was operated on due to tongue squamous cell carcinoma (SCC) on the left side T3N1M0 in 2019. Bilateral selective neck dissection with tongue reconstruction was performed by island FAMM flap. The patient also suffered from synchronous mucinous breast carcinoma treated with tamoxifen. The second primary SCC of the tongue on the opposite (right) side was detected in 2020. The patient did not agree to surgical treatment; therefore, radiotherapy was performed. The local recurrence of the tongue cancer of the right side was treated surgically in 2021. Salvage surgery comprised hemiglossectomy and dpFAMM flap reconstruction with uneventful postoperative follow-up. Conclusions This case presentation proved that dpFAMM flap can be used in salvage surgery and reconstruction even in patients after ligation of facial vessels, irradiation, and in the course of hormone therapy. The flap is easy to handle, has good vascularity, and comprises a predictable method of reconstruction, especially for patients with severe comorbidities.

The implementation of algorithmic decision-making systems in public administration can pose a risk to the rule of law, democracy, and the right to good administration. Risk management in this regard is intended to be enabled by the instruments provided for in the European draft act on artificial intelligence. The aim of this study is to establish the legal situation of an individual on the grounds of the aforementioned act, and then to relate it to the assumptions of the legislative proposal of the European Law Institute and the provisions of Polish administrative law. The research uses a dogmatic-legal method and a comparative method with regard to references to the common law in force in the United Kingdom. The analysis shows that the European draft regulation completely ignores the individual by not granting him/her legal remedies against the implementation of the said systems. Its protection could be strengthened by allowing complaints to the supervisory authority, whose decisions would be subject to review by administrative courts, as is the case under the provisions of the General Data Protection Regulation. Adoption of these solutions to the Polish legal order could complement the already existing model of control of partly automatic decisions of the administration, providing the possibility to challenge also the principles of their adoption. This would, however, require a determination of who would have a legal interest in lodging a complaint, the possibility for social organisations to participate in these proceedings, and the relationship to other legal instruments already in place, such as public consultations in the legislative process and impact assessments.

Although the interactors of pluripotency factors have been identified in mouse embryonic stem cells (ESCs), their interactors in human ESCs remain unexplored. Here the authors map OCT4 protein interactions in naïve and primed human ESCs to find specific interactions with BAF subunits that promote an open chromatin architecture at blastocyst-associated genes and ectodermal genes, respectively.

Abstract Background Copy number variants (CNVs) linked to genes involved in nervous system development or function are often associated with neuropsychiatric disease. While CNVs involving deletions generally cause severe and highly penetrant patient phenotypes, CNVs leading to duplications tend instead to exhibit widely variable and less penetrant phenotypic expressivity among affected individuals. CNVs located on chromosome 15q13.3 affecting the alpha-7 nicotinic acetylcholine receptor subunit (CHRNA7) gene contribute to multiple neuropsychiatric disorders with highly variable penetrance. However, the basis of such differential penetrance remains uncharacterized. Here, we generated induced pluripotent stem cell (iPSC) models from first-degree relatives with a 15q13.3 duplication and analyzed their cellular phenotypes to uncover a basis for the dissimilar phenotypic expressivity. Results The first-degree relatives studied included a boy with autism and emotional dysregulation (the affected proband-AP) and his clinically unaffected mother (UM), with comparison to unrelated control models lacking this duplication. Potential contributors to neuropsychiatric impairment were modeled in iPSC-derived cortical excitatory and inhibitory neurons. The AP-derived model uniquely exhibited disruptions of cellular physiology and neurodevelopment not observed in either the UM or unrelated controls. These included enhanced neural progenitor proliferation but impaired neuronal differentiation, maturation, and migration, and increased endoplasmic reticulum (ER) stress. Both the neuronal migration deficit and elevated ER stress could be selectively rescued by different pharmacologic agents. Neuronal gene expression was also dysregulated in the AP, including reduced expression of genes related to behavior, psychological disorders, neuritogenesis, neuronal migration, and Wnt, axonal guidance, and GABA receptor signaling. The UM model instead exhibited upregulated expression of genes in many of these same pathways, suggesting that molecular compensation could have contributed to the lack of neurodevelopmental phenotypes in this model. However, both AP- and UM-derived neurons exhibited shared alterations of neuronal function, including increased action potential firing and elevated cholinergic activity, consistent with increased homomeric CHRNA7 channel activity. Conclusions These data define both diagnosis-associated cellular phenotypes and shared functional anomalies related to CHRNA7 duplication that may contribute to variable phenotypic penetrance in individuals with 15q13.3 duplication. The capacity for pharmacological agents to rescue some neurodevelopmental anomalies associated with diagnosis suggests avenues for intervention for carriers of this duplication and other CNVs that cause related disorders.

[1] Background: Congenital superficial ranulas (CSR) are very uncommon entities in neonates, corresponding to 0.74% in contrast to lingual frenulum alterations ranging from 2.8 to 10.7%. Both anomalies should be appropriately diagnosed and treated [2]. Methods: This paper introduces the case of an 8-week infant with CSR and short lingual frenulum, successfully managed by surgical marsupialization during a frenotomy procedure. After the comprehensive oral examination, we have identified the primary cause of feeding issues. Ranula was diagnosed by classic clinical presentation, ultrasound, and presence of typical mucous during the surgical management [3]. Results: The healing was uneventful, and we did not observe recurrence during the 6-month follow-up. Post-surgery, the infant could breastfeed easily [4]. Conclusion: CSR in case of concomitant ankyloglossia should be identified and surgically treated by marsupialization in the first 6 months before transitioning to solid food.

Autoimmune thyroid disease (AITD) is more common among diabetes mellitus (DM) patients and may impact its microvascular complications. The present study aimed to assess the relationship between AITD and the prevalence of diabetic kidney disease (DKD) in patients with diabetes mellitus type 1 (DM1). Anthropometric parameters, parameters of metabolic control of DM, thyreometabolic status, and the UACR were assessed. DKD was diagnosed if patients’ UACR level was ≥30 mg/g or eGFR level was

Hashimoto’s disease (HD) is the most common cause of hypothyroidism in developed countries. The exact pathomechanism behind it has not been clearly established; however, an interplay of genetic susceptibility, environmental triggers (including diet) and epigenetic factors seems to be involved. Among the latter, increasingly more attention has been paid to some hormonally active substances, known as endocrine disruptors, which are commonly used worldwide. HD has become a condition widely reported in the media, acting as a culprit for inexplicable weight gain, chronic fatigue or weakness. Nevertheless, the recognition of HD is undeniably increasing and represents a major public health burden. At the same time, improving access to imaging tests has increased the number of incidentally diagnosed adrenal tumors. Above all, the widespread use of chest computed tomography (CT) due to the COVID-19 pandemic has contributed to frequent incidental detection of adrenal lesions. Fortunately, a vast majority of these findings are asymptomatic benign tumors with no excessive hormonal activity, and therefore, they are defined as adrenal incidentalomas (AIs). Interestingly, recent studies have indicated that patients with AIs are more prone to obesity and insulin resistance. Although mutual relationships between the thyroid and the adrenal glands have been studied widely, still, little is known about the possible pathophysiological associations between thyroid autoimmunity and the occurrence of adrenal incidentalomas. This article presents a brief review of the common endocrine disorders with a special focus on the frequently coexisting insulin resistance and/or obesity. Furthermore, in response to the recent growing interest in endocrine disruptors, with their transgenerational epigenetic effects that influence hormonal system function, a concise overview of the topic has also been included.

Introduction Extraparotid Warthin tumor (WT) is a very rare entity, especially when synchronous with oral cancer (OC). Objective The present study presents a case series of extraparotid WTs detected in the surgical specimen of patients treated for OC. Methods From 2007 to 2016, 336 patients were operated for OC in our institution. Neck dissection was performed in 306 patients. Results In the 306 patients operated for OC whose necks were dissected, unexpected WTs were observed in 4 surgical neck specimens. In 3 cases, extraparotid WTs were responsible for tumor, node, metastasis (TNM) overstaging before surgery. Conclusion Extraparotid WTs may be discovered during neck dissection in ∼ 1% of OC patients, and they may mimic neck metastasis, especially in positron-emission tomography/computed tomography (PET/CT) imaging.

Abstract Background Transposable elements (TEs) are a significant component of eukaryotic genomes and play essential roles in genome evolution. Mounting evidence indicates that TEs are highly transcribed in early embryo development and contribute to distinct biological functions and tissue morphology. Results We examine the epigenetic dynamics of mouse TEs during the development of five tissues: intestine, liver, lung, stomach, and kidney. We found that TEs are associated with over 20% of open chromatin regions during development. Close to half of these accessible TEs are only activated in a single tissue and a specific developmental stage. Most accessible TEs are rodent-specific. Across these five tissues, 453 accessible TEs are found to create the transcription start sites of downstream genes in mouse, including 117 protein-coding genes and 144 lincRNA genes, 93.7% of which are mouse-specific. Species-specific TE-derived transcription start sites are found to drive the expression of tissue-specific genes and change their tissue-specific expression patterns during evolution. Conclusion Our results suggest that TE insertions increase the regulatory potential of the genome, and some TEs have been domesticated to become a crucial component of gene and regulate tissue-specific expression during mouse tissue development.

Durum wheat is the tenth most valuable crop on a global scale. The aim of this study was to compare the phenotypic variation of T. durum accessions of different origin with contemporary spring cultivars of this cereal species. One hundred and two accessions and 12 contemporary cultivars of Triticum durum Desf. as well as Kamut® wheat (T. turanicum), a Triticum species closely related to T. durum, were analyzed. The aim of this study was to describe the degree of variation in the phenotypic traits of grain and selected traits associated with technological quality. The examined genotypes were characterized by considerable phenotypic variation, and they can be a valuable source of material for genetic recombination in durum wheat breeding. The analyzed accessions were characterized by a higher average content of protein (16.48 vs. 14.56%) and wet gluten (38.04 vs. 32.07%), higher Zeleny sedimentation values (69.7 vs. 60.4ml), and higher flour strength (W index values of 404.64 vs. 353.47) than the reference cultivars. The kernels of the evaluated accessions and cultivars did not differ significantly in average crease depth, but significant differences were observed in the values of descriptors directly linked with kernel size, especially kernel image area and minimal Feret diameter. The traits responsible for the processing suitability of grain were more strongly correlated with color descriptors than shape descriptors, which suggests that color parameters can be used to select high-quality breeding material. The analyzed accessions have two major weaknesses, namely relatively low yields (22.6 dt ha-1 on average) and undesirable grain color, indicative of low carotenoid concentration. The accessions deposited in gene banks do not meet the relevant agronomic requirements. However, both grain yield and carotenoid concentration are polygenic traits which can be improved if desirable combinations of QTLs are assembled in breeding lines and cultivars.

Durum wheat grain can accumulate mycotoxins because it is highly sensitive to infections caused by pathogens of the genera Fusarium and Alternaria. Reduced fungicide use increases the demand for biological methods of pathogen control. The aim of the experiment was to evaluate the efficacy of Debaryomyces hansenii (Dh) yeast in reducing the content of secondary fungal metabolites present in the spikes of five durum wheat cultivars grown in southern and northern Poland. A total of 27 Fusarium metabolites and nine metabolites produced by other fungi were identified in the grain. The application of the Dh yeast strain decreased deoxynivalenol concentration in all samples relative to control treatments (by 14–100%) and treatments inoculated with F. graminearum (by 23–100%). In northern Poland, the biological treatment also led to a considerable reduction in the content of culmorin (by 83.2–100%) and enniatins A1 and B (by 9.5–65.3% and 6.7–70%, respectively) in the grain. An analysis of multiple fungal metabolites is a highly useful tool for determining grain quality and its suitability for consumption. When applied in the flowering stage, yeasts can partly complete fungicides in reducing Fusarium head blight.

Cystic lesions observed in parotid glands are relatively rare and comprise 2–5% of all parotid primaries. A salivary duct cyst (SDC) is a true cyst representing 10% of all salivary gland cysts. The risk of malignant transformation of SDC’s epithelium is extremely rare. In the literature, only three cases of carcinoma ex SDC of the parotid gland are described. This report presents the first in the literature case of myoepithelial carcinoma (MECA) arising from a parotid SDC. A 75-year-old male patient was referred to the Department of Cranio-Maxillofacial Surgery of the Jagiellonian University in Cracow, Poland due to a cystic tumor arising from the right parotid gland. Superficial parotidectomy with facial nerve preservation was performed. Histological examination confirmed a rare case of MECA emerging from the SDC. The immunohistochemical profile of MECA ex SDC was presented. During 6 months of the follow-up, local recurrence or distant metastasis was not observed.

Prosthetic rehabilitation has hardly changed since the 1970s, and it is a time-consuming process that requires great skill and precision. This article presents the workflow related to the prosthetic reconstruction of patients with extensive maxillofacial defects using digital technologies such as cone beam computed tomography (CBCT), Zbrush software and three-dimensional (3D) printing. The use of CBCT captures a 3D image of a patient’s face in a few seconds, thus shortening the time to prepare an impression and increasing the patient’s comfort. Zbrush digital sculpting software enables one to quickly and efficiently design a prosthetic restoration. Printing a trial version for a try-on, as well as designing and printing pre-cast forms, improves workflow and eliminates the chances of an error. As shown in the example of a patient after a nasal resection, CBCT, digital sculpting, and 3D printing accelerate and improve the workflow of an anaplastologist when designing the epithesis. New technologies reduce costs and save time, while helping to overcome the technical obstacles that limit access to a facial prosthesis.

The present paper reports the results of theoretical and experimental studies of the process of die forging a bimetallic door handle intended for the production of a helicopter. The aim of the studies was to develop and implement a technology for die forging of a product with a specific mass similar to that of magnesium alloys which will have, however higher corrosion resistance. Numerical modelling and industrial tests were carried out based on the previously forging processes for an AZ31 alloy door handle. The material for the tests was a bimetallic bar produced by the explosive welding method, in which the core was of alloy AZ31, and the cladding layer was made of 1050A grade aluminium. The studies were conducted for two variants: Variant I – the forging process was mapped by numerical modelling and industrial tests for the die shape and parameters used in the forging of the AZ31 alloy door handle, Variant II – the tool shape was optimized and process parameters were selected so as to obtain a finished product characterized by a continuous Al layer. From the theoretical studies and experimental tests carried out it has been found that the application of the Variant I does not assure that a finished door handle characterized by a continuous cladding layer will be produced. Within this study, a novel method of bimetallic door handle die forging (Variant II) has been developed, which limits the amount of the flash formed and assures the integrity of the cladding layer.

Abstract Background Autism spectrum disorder (ASD) is a neurodevelopmental disorder with pronounced heritability in the general population. This is largely attributable to the effects of polygenic susceptibility, with inherited liability exhibiting distinct sex differences in phenotypic expression. Attempts to model ASD in human cellular systems have principally involved rare de novo mutations associated with ASD phenocopies. However, by definition, these models are not representative of polygenic liability, which accounts for the vast share of population-attributable risk. Methods Here, we performed what is, to our knowledge, the first attempt to model multiplex autism using patient-derived induced pluripotent stem cells (iPSCs) in a family manifesting incremental degrees of phenotypic expression of inherited liability (absent, intermediate, severe). The family members share an inherited variant of uncertain significance (VUS) in GPD2, a gene that was previously associated with developmental disability but here is insufficient by itself to cause ASD. iPSCs from three first-degree relatives and an unrelated control were differentiated into both cortical excitatory (cExN) and cortical inhibitory (cIN) neurons, and cellular phenotyping and transcriptomic analysis were conducted. Results cExN neurospheres from the two affected individuals were reduced in size, compared to those derived from unaffected related and unrelated individuals. This reduction was, at least in part, due to increased apoptosis of cells from affected individuals upon initiation of cExN neural induction. Likewise, cIN neural progenitor cells from affected individuals exhibited increased apoptosis, compared to both unaffected individuals. Transcriptomic analysis of both cExN and cIN neural progenitor cells revealed distinct molecular signatures associated with affectation, including the misregulation of suites of genes associated with neural development, neuronal function, and behavior, as well as altered expression of ASD risk-associated genes. Conclusions We have provided evidence of morphological, physiological, and transcriptomic signatures of polygenic liability to ASD from an analysis of cellular models derived from a multiplex autism family. ASD is commonly inherited on the basis of additive genetic liability. Therefore, identifying convergent cellular and molecular phenotypes resulting from polygenic and monogenic susceptibility may provide a critical bridge for determining which of the disparate effects of rare highly deleterious mutations might also apply to common autistic syndromes.

The article describes a computer analysis of the pull-out test used to calculate the force needed to pull out a rock fragment and determine the shape of this broken fragment. The analyzed material is sandstone and porphyry. The analysis included the first approach to using own subroutine in the Simulia Abaqus system, that is, which task is undertaken to accurately determine the crack path of the Finite Element Method model. The work also contains a description of laboratory tests and analytical considerations.

Introduction. Diabetes mellitus contributes to the development of microvascular complications in the eye. Moreover, it affects multiple end organs, including brain damage, leading to premature death. The use of adaptive optics technique allows to perform non-invasive in vivo assessment of retinal vessels and to identify changes in arterioles about 100 μm in diameter. The retinal vasculature may be a model of the cerebral vessels both morphologically and functionally. Aim. To evaluate morphological parameters of retinal arterioles in patients with type 1 diabetes mellitus (DM1). Material and methods. The study included 22 DM1 patients (13 females) aged 43.00 ± 9.45 years with a mean diabetes duration of 22.55 ± 10.05 years, and 23 healthy volunteers (10 females) aged 41.09 ± 10.99 years. Blood pressure, BMI, waist circumference, and metabolic control markers of diabetes were measured in both groups. Vascular examinations were performed using an rtx1 adaptive optics retinal camera (Imagine Eyes, Orsay, France); the vessel wall thickness (WT), lumen diameter (LD), wall-to-lumen ratio (WLR), and vascular wall cross-sectional area (WCSA) were assessed. Statistical analysis was performed with the application of IMB SPSS version 23 software. Results. The DM1 group did not differ significantly in age, BMI, waist circumference, blood pressure, or axial length of the eye compared to the control group. Intraocular pressure (IOP) in both groups was normal, but in the DM1 group it was significantly higher. The DM1 group had significantly higher WT, WLR, and WCSA. These parameters correlated significantly with the duration of diabetes, but not with IOP. Conclusions. The presented study demonstrates the presence of significant morphological changes in retinal vessels in DM1 patients without previously diagnosed diabetic retinopathy. Similar changes may occur in the brain and may be early indicators of cardiovascular risk, but further investigation is required to confirm that.

AbstractThe mechanisms that determine the final topology of skeletal muscles remain largely unknown. We have been developing Drosophilaback seat driver, kon-tiki, thisbetumbleweedspalt-majorbarrenpatched