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10. Frontal analysis capillary electrophoresis hyphenated to electrospray ionization mass spectrometry for the characterization of the antithrombin/heparin pentasaccharide complex

Author

Fermas, Soraya, Gonnet, Florence, Varenne, Anne, Gareil, Pierre, and Daniel, Regis

Subjects
Mass spectrometry -- Methods, Electrophoresis -- Methods, Antithrombins -- Chemical properties, Antithrombins -- Identification and classification, Heparin -- Identification and classification, Heparin -- Chemical properties, Ligands (Biochemistry) -- Identification and classification, Chemistry
Abstract

The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. A new strategy based on the hyphenation of frontal analysis capillary electrophoresis (FACE) with electro-spray ionization mass spectrometry (ESIMS) is reported for the characterization of protein/carbohydrate complexes. While most of the previously reported CE-MS experiments were performed using capillary electrophoresis in zone format, we report for the first time CE-MS experiments in which CE was performed in frontal analysis (FACE-MS). We showed that the frontal mode offered a better sensitivity than zone mode and was well suited for the CE-MS coupling. This FACE-MS coupling was applied to the analysis of the complex between antithrombin and the suffated pentasaccharide reproducing the antithrombin-binding sequence in heparin. The mixture of coincubated antithrombin and heparin pentasaccharide was continuously injected into the capillary, and the electrophoretic separation of the free and bound forms of the protein was achieved. The intact noncovalent complex antithrombin/heparin pentasaccharide was detected on-line by ESIMS in positive ionization mode and in nondenaturing sheath liquid conditions. The complex stoichiometry was determined from the mass measurement of the complex. In addition, the characterization of the sulfated pentasaccharide ligand dissociated from the complex was performed in negative ionization mode using a denaturing sheath liquid, allowing the determination of its molecular mass and sulfation features. This FACE-ESIMS strategy opens the way to ligand fishing experiments performed on heterogeneous carbohydrate mixtures and subsequent characterization of specifically bound carbohydrates.

Published
2007

11. Mass spectrometry analysis of the oligomeric C1q protein reveals the B chain as the target of trypsin cleavage and interaction with fucoidan

Author

Tissot, Berangere, Gonnet, Florence, Iborra, Alban, Berthou, Christian, Thielens, Nicole, Arlaud, Gerard J., and Daniel, Regis

Subjects
Binding proteins -- Research, Polysaccharides -- Research, Trypsin -- Research, Mass spectrometry -- Usage, Biological sciences, Chemistry
Abstract

C1q is a subunit of the C1 complex that triggers activation of the complement classical pathway through recognition and binding of immune complexes and C1q was submitted for the first time to mass spectrometry analysis, yielding insights into its assembly and its interaction with fucoidan. Unlike DNA, fucoidan protected C1q from trypsin cleavage, indicating that this polysaccharide binds to the B moiety of the globular head.

Published
2005

19. Synthesis, structure, and characterization of the new (L(OH)Fe(mu-O)Fe(OH2)L)3+ complex (L = N.N'-dimethyl-N,N'-bis(2-pyridylmethyl)ethane-1,2-diamine). Detection of an equilibrium with the protonated diamond form (LFe(mu-O)(mu-OH)FeL)3+ in acetonitrile

Author

Poussereau, Sandrine, Blondin, Genevieve, Cesario, Michele, Guilhem, Jean, Chottard, Genevieve, Gonnet, Florence, and Girerd, Jean-Jacques

Subjects
Metalloproteins -- Research, Binding sites (Biochemistry) -- Research, Water -- Analysis, Chemistry
Abstract

The protonated diamond core (LFe(mu-O)(mu-OH)FeL)3+ is produced when the mono-mu-oxo bridging species (L(H2O)Fe(mu-O)Fe(OH)L)3+ loses its water molecule in acetonitrile spontaneously, a reaction reversible by addition of water. This was gleaned from the characterization of a new complex containing the hydrogen-bonded ((H2O)Fe(mu-O)Fe(OH))3+ core unit. The sensitivity of the protonated diamond core to the presence of water suggests that there could be a relationship between the stability of the diiron diamond core in methane monooxygenase and the hydrophobicity of the active site.

Published
1998

20. Reactions of single-stranded oligonucleotides with cis-(Pt NH3 sub2 H20 sub 2)2+ and (Pt NH3 sub3 H20)2+

Author

Gonnet, Florence, Reeder, Franziska, Kozelka, Jiri, and Chottard, Jean-Claude

Subjects
Platinum compounds -- Research, Nucleic acids -- Research, Chemical reaction, Rate of -- Research, Chelates -- Research, Ligands -- Research, Chemistry
Abstract

The kinetics of platination reactions between single-stranded oligonucleotides containing a d(GG) sequence and cis- Pt(NH3)2(H2O)2 (super 2+) and Pt(NH3)3(H2O) (super 2+) have been investigated. The results of concentration measurements based on high performance liquid chromatography showed the increase of platination rate with k5' > k3' with the increase in oligonucleotide chain length. The platination rate decreased when the OH2 ligand was replaced by NH3.

Published
1996

21. Characterization of Renal Injury and Inflammation in an Experimental Model of Intravascular Hemolysis

Author

Merle, Nicolas, Grunenwald, Anne, Figueres, Marie-Lucile, Chauvet, Sophie, Daugan, Marie, Knockaert, Samantha, Robe-Rybkine, Tania, Noe, Remi, May, Olivia, Frimat, Marie, Brinkman, Nathan, Gentinetta, Thomas, Miescher, Sylvia, Houillier, Pascal, Legros, Véronique, Gonnet, Florence, Blanc-Brude, Olivier, Rabant, Marion, Daniel, Régis, Dimitrov, Jordan, Roumenina, Lubka, Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), CSL Behring, Service de Physiologie [Georges-Pompidou], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5), Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Saclay (COmUE), Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), ANR-15-CE15-0001,INFLACOMP,Inflammation et lésions endothéliales induites par le Complément et l'hémolyse(2015), ANR-12-JSV1-0006,PIKinART,Role de la phosphoinositide 3-kinase dans les pathologies artérielles: une cible pharmacologique prometteuse(2012), ANR-11-IDEX-0005,USPC,Université Sorbonne Paris Cité(2011), École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5), Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE - UMR 8587), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université d'Évry-Val-d'Essonne (UEVE)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine, Centre de Recherche Cardiovasculaire de Lariboisiere, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-15-CE15-0001,INFLACOMP,Inflammation et lésions endothéliales induites par le Complément et l’hémolyse(2015), ANR-11-IDEX-0005-02/11-IDEX-0005,USPC,USPC(2011), École Pratique des Hautes Études (EPHE), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Lille Inflammation Research International Center (LIRIC), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Paris 5 (UPD5)-Hôpital Européen Georges Pompidou [APHP] (HEGP), and Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Erythrocytes, hemopexin, Immunology, endothelial activation, Kidney, phenylhydrazine, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, [SDV.MHEP.UN]Life Sciences [q-bio]/Human health and pathology/Urology and Nephrology, Mice, Lipocalin-2, Human Umbilical Vein Endothelial Cells, Animals, Humans, Hepatitis A Virus Cellular Receptor 1, Vascular Diseases, heme, Cells, Cultured, Original Research, Acute Kidney Injury, Phenylhydrazines, Mice, Inbred C57BL, Disease Models, Animal, Ki-67 Antigen, inflammation, kidney injury, Female, hemolysis, E-Selectin, Biomarkers, experimental model of intravascular hemolysis
Abstract

International audience; Intravascular erythrocyte destruction, accompanied by the release of pro-oxidative and pro-inflammatory components hemoglobin and heme, is a common event in the pathogenesis of numerous diseases with heterogeneous etiology and clinical features. A frequent adverse effect related to massive hemolysis is the renal injury and inflammation. Nevertheless, it is still unclear whether heme––a danger-associated molecular pattern––and ligand for TLR4 or upstream hemolysis-derived products are responsible for these effects. Well-characterized animal models of hemolysis with kidney impairment are needed to investigate how hemolysis drives kidney injury and to test novel therapeutic strategies. Here, we characterized the pathological processes leading to acute kidney injury and inflammation during massive intravascular hemolysis, using a mouse model of phenylhydrazine (PHZ)-triggered erythrocyte destruction. We observed profound changes in mRNA levels for markers of tubular damage (Kim-1, NGAL) and regeneration (indirect marker of tubular injury, Ki-67), and tissue and vascular inflammation (IL-6, E-selectin, P-selectin, ICAM-1) in kidneys of PHZ-treated mice, associated with ultrastructural signs of tubular injury. Moreover, mass spectrometry revealed presence of markers of tubular damage in urine, including meprin-α, cytoskeletal keratins, α-1-antitrypsin, and α-1-microglobulin. Signs of renal injury and inflammation rapidly resolved and the renal function was preserved, despite major changes in metabolic parameters of PHZ-injected animals. Mechanistically, renal alterations were largely heme-independent, since injection of free heme could not reproduce them, and scavenging heme with hemopexin in PHZ-administered mice could not prevent them. Reduced overall health status of the mice suggested multiorgan involvement. We detected amylasemia and amylasuria, two markers of acute pancreatitis. We also provide detailed characterization of renal manifestations associated with acute intravascular hemolysis, which may be mediated by hemolysis-derived products upstream of heme release. This analysis provides a platform for further investigations of hemolytic diseases and associated renal injury and the evaluation of novel therapeutic strategies that target intravascular hemolysis.

Published
2018

23. MALDI/MS peptide mass fingerprinting for proteome analysis: identification of hydrophobic proteins attached to eucaryote keratinocyte cytoplasmic membrane using different matrices in concert

Author

Waksman Gilles, Lemaître Gilles, Gonnet Florence, and Tortajada Jeanine

Subjects
Cytology, QH573-671
Abstract

Abstract Background MALDI-TOF-MS has become an important analytical tool in the identification of proteins and evaluation of their role in biological processes. A typical protocol consists of sample purification, separation of proteins by 2D-PAGE, enzymatic digestion and identification of proteins by peptide mass fingerprint. Unfortunately, this approach is not appropriate for the identification of membrane or low or high pI proteins. An alternative technique uses 1D-PAGE, which results in a mixture of proteins in each gel band. The direct analysis of the proteolytic digestion of this mixture is often problematic because of poor peptide detection and consequent poor sequence coverage in databases. Sequence coverage can be improved through the combination of several matrices. Results The aim of this study was to trust the MALDI analysis of complex biological samples, in order to identify proteins that interact with the membrane network of keratinocytes. Peptides obtained from protein trypsin digestions may have either hydrophobic or hydrophilic sections, in which case, the direct analysis of such a mixture by MALDI does not allow desorbing of all peptides. In this work, MALDI/MS experiments were thus performed using four different matrices in concert. The data were analysed with three algorithms in order to test each of them. We observed that the use of at least two matrices in concert leads to a twofold increase of the coverage of each protein. Considering data obtained in this study, we recommend the use of HCCA in concert with the SA matrix in order to obtain a good coverage of hydrophilic proteins, and DHB in concert with the SA matrix to obtain a good coverage of hydrophobic proteins. Conclusion In this work, experiments were performed directly on complex biological samples, in order to see systematic comparison between different matrices for real-life samples and to show a correlation that will be applicable to similar studies. When 1D gel is needed, each band may contain a great number of proteins, each present in small amounts. To improve the proteins coverage, we have performed experiments with some matrices in concert. These experiments enabled reliable identification of proteins, without the use of Nanospray MS/MS experiments.

Published
2003
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25. BIOPUCES FONCTIONNALISEES POUR LE COUPLAGE SPR-MS

Author

Daniel, Régis, Gonnet, Florence, Buchmann, William, Bellon, Sophie, Jarroux, Nathalie, Anger-Leroy, Marielle, Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Daniel, Régis, and Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics
Abstract

The invention relates to a method for coupling in-line the analysis of molecular interactions by surface plasmon resonance (SPR) with a structural identification by mass spectrometry using the same functionalised medium for both types of analysis., L'invention concerne un procédé permettant de coupler en ligne l'analyse des interactions moléculaires par résonance de plasmons de surface (SPR) à une identification structurale par spectrométrie de masse, en utilisant un même support fonctionnalisé pour les deux types d'analyse.

Published
2016

26. A novel CFHR1-CFHR5 hybrid leads to a familial dominant C3 glomerulopathy

Author

Togarsimalemath, Shambhuprasad K., primary, Sethi, Sidharth K., additional, Duggal, Rajan, additional, Le Quintrec, Moglie, additional, Jha, Pranaw, additional, Daniel, Régis, additional, Gonnet, Florence, additional, Bansal, Shyam, additional, Roumenina, Lubka T., additional, Fremeaux-Bacchi, Veronique, additional, Kher, Vijay, additional, and Dragon-Durey, Marie-Agnes, additional

Published
2017
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29. The chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from marine bacterium Vibrio sp FC509 is a dimeric species: Biophysical characterization of an endosulfatase

Author

Neira, José L., primary, Medina-Carmona, Encarnación, additional, Hernández-Cifre, José G., additional, Montoliu-Gaya, Laia, additional, Cámara-Artigás, Ana, additional, Seffouh, Ilham, additional, Gonnet, Florence, additional, Daniel, Régis, additional, Villegas, Sandra, additional, de la Torre, José García, additional, Pey, Angel L., additional, and Li, Fuchuan, additional

Published
2016
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30. Synchrotron X-ray footprinting – Mass-spectrometry to probe structure of proteins in interactions

Author

Gohon, Yann, Jolivet, Pascale, Froissard, Marine, Tâche, Roselyne, Chardot, Thierry, Giuliani, Alexandre, Mercere, Pascal, Vermachova, Martina, Młynarczyk, A. H., Daniel, Régis, Gonnet, Florence, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris Saclay (COmUE), Synchrotron SOLEIL, Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement (LAMBE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, and Université Paris-Seine-Université Paris-Seine-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering
Abstract

National audience; The meeting provides an invaluable forum for the synchrotron radiation user community, presenting an important opportunity to obtain the latest information on beamline performance at SOLEIL, to hear about the latest and most exciting results obtained at SOLEIL and to share scientific, technical and practical issues about the synchrotron radiation use.

Published
2015

33. Corrigendum to “Alteration of cerebral iron metabolism in Sanfilippo syndrome” [Mol. Genet. Metab. 117 2, February 2016, p. S97]

Author

Puy, Vincent, primary, Bodet, Pierre-Edouard, additional, Rottier, Camille, additional, Delaunay, Rémi, additional, Gomila, Cathy, additional, Hodroge, Ahmed, additional, Rackowski, Liliane, additional, Lefebvre, Thibaud, additional, Karim, Zoubida, additional, Vitry, Sandrine, additional, Gonnet, Florence, additional, Daniel, Régis, additional, and Ausseil, Jerome, additional

Published
2016
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34. Complexes de Manganèse à haut degré d'oxydation. Oxydation chimique et éléctrochimique de complexes Mn(II)

Author

Hureau, Christelle, Lassalle, Benedikt, Gonnet, Florence, Sabater, Laurent, Blain, Guillaume, Philouze, Christian, Blondin, Geneviève, Anxolabéhère-Mallart, Elodie, Laboratoire de chimie inorganique (LCI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'études dynamiques et structurales de la sélectivité (LEDSS), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Laboratoire de Physico-chimie des Métaux en Biologie (LPCMB), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), and Gonnet, Florence

Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry
Abstract

Affiche

Published
2005

35. Etude des interactions bio-moléculaires au LAE: apport de la spectrométrie de masse et de méthodes d'analyse bio-physiques

Author

Daniel, Régis, Gonnet, Florence, Buchmann, William, Tortajada, Jeanine, Gonnet, Florence, Analyse et environnement (LAE), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Published
2005

36. Mass spectrometry analysis of complement protein C1q in the study of its interaction properties with fucoidan

Author

Tissot, Bérangère, Gonnet, Florence, Iborra, Alban, Berthou, Christian, Thielens, Nicole, Arlaud, Gérard J, Daniel, Régis, Laboratoire de Thérapie Cellulaire, CHU Morvan, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d' Enzymologie Moléculaire (LEM), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Published
2005

37. The search of new markers of keratinocyte differentiation

Author

Lemaitre, Gilles, Gonnet, Florence, Lamartine, J., Le Minter, P., Vaigot, Pierre, Gidrol, Xavier, Tortajada, Jeanine, Martin, Michèle, Waksman, Gilles, Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Published
2005

38. Isolement et caractérisation de marqueurs membranaires des cellules progénitrices de l'épiderme humain

Author

Lemaitre, Gilles, Serhal, H., Cohen, Albert, Bourdin, G., Gonnet, Florence, Vaigot, Pierre, Rachidi, Walid, Tortajada, Jeanine, Martin, Michèle, Waksman, Gilles, Gonnet, Florence, Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Analyse et environnement (LAE), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche

Published
2005

39. Analyse par spectrométrie de masse du pont thio-ester intramoléculaire de la protéine C3 du système du complément et de ses adduits covalents avec des composés inhibiteurs

Author

Bouitier, Julie, Zirah, Séverine, Tissot, Bérangère, Gonnet, Florence, Daniel, Régis, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche

Published
2005

40. Analyse directe par UV-MALDI-TOF de la matrice extra-cellulaire de cellules cultivées sur membrane polyester

Author

Zirah, Séverine, Gonnet, Florence, Lemaitre, Gilles, Waksman, Gilles, Tortajada, Jeanine, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche

Published
2004

41. Apport de la spectrométrie de masse à l'étude structurale du complexe protéique C1q du système du Complément

Author

Iborra, Alban, Tissot, Bérangère, Tortajada, Jeanine, Gonnet, Florence, Daniel, Régis, Gonnet, Florence, Analyse et environnement (LAE), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
stomatognathic diseases, InformationSystems_MODELSANDPRINCIPLES, ComputingMilieux_THECOMPUTINGPROFESSION, GeneralLiterature_INTRODUCTORYANDSURVEY, ComputingMilieux_COMPUTERSANDEDUCATION, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Communication Orale

Published
2004

42. Vers l'obtention d'ions multichargés en UV-MALDI-TOF

Author

Zirah, Séverine, Gonnet, Florence, Tortajada, Jeanine, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche

Published
2004

43. Obtention de complexes Mn-oxo par oxidations chimiques et électrochimiques

Author

Hureau, Christelle, Sabater, Laurent, Lassalle, Benedikt, Anxolabéhère-Mallart, Elodie, Blondin, Geneviève, Gonnet, Florence, Nierlich, Martine, Philouze, Christian, Laboratoire de chimie inorganique (LCI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Département de Recherche sur l'Etat Condensé, les Atomes et les Molécules (DRECAM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire d'études dynamiques et structurales de la sélectivité (LEDSS), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), and Gonnet, Florence

Subjects
[CHIM.MATE] Chemical Sciences/Material chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry
Abstract

Affiche

Published
2004

44. Proteomic analysis of caveolin1 enriched fractions of membrane proteins of human keratinocytes

Author

Gonnet, Florence, Lemaitre, Gilles, Gidrol, Xavier, Waksman, Gilles, Tortajada, Jeanine, Gonnet, Florence, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Service de Génomique Fonctionnelle, and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche

Published
2003

45. Enhanced sequence coverage of proteins interacting with plasma membrane of keratinocytes by using MALDI with different matrices in concert

Author

Gonnet, Florence, Lemaitre, Gilles, Gidrol, Xavier, Waksman, Gilles, Tortajada, Jeanine, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche

Published
2003

46. Identification de protéines membranaires par spectrométrie de masse

Author

Gonnet, Florence, Lemaitre, Gilles, Waksman, Gilles, Tortajada, Jeanine, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Published
2003

47. Purification of caveolae microdomains from HaCaT keratinocytes followed by proteomic analysis allows the characterization of new markers of human epidermis layers

Author

Lemaitre, Gilles, Gonnet, Florence, Vaigot, Pierre, Gidrol, Xavier, Tortajada, Jeanine, Waksman, Gilles, Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), ASBMB, and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche; International audience

Published
2003

48. Analyse protéomique de fractions membranaires de kératinocytes eucaryotes

Author

Gonnet, Florence, Lemaitre, Gilles, Waksman, Gilles, Tortajada, Jeanine, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Published
2003

49. Etude protéomique d'une fraction membranaire de kératinocytes enrichie en microdomaine caveolae

Author

Gonnet, Florence, Lemaitre, Gilles, Waksman, Gilles, Tortajada, Jeanine, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Published
2003

50. Isolement et caractérisation de protéines membranaires des kératinocytes

Author

Gonnet, Florence, Lemaitre, Gilles, Waksman, Gilles, Tortajada, Jeanine, Analyse et environnement (LAE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Gonnet, Florence

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

Affiche

Published
2002

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