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4. FOXL2regulates RhoAexpression to change actin cytoskeleton rearrangement in granulosa cells of chicken pre-ovulatory follicles†

Author

Li, Xuelian, Du, Hongting, Zhou, Haobo, Huang, Ying, Tang, Shuixin, Yu, Chengzhi, Guo, Yan, Luo, Wei, and Gong, Yanzhang

Abstract

Forkhead box L2 (FOXL2) is an indispensable key regulator of female follicular development, and it plays important roles in the morphogenesis, proliferation, and differentiation of follicle granulosa cells, such as establishing normal estradiol signaling and regulating steroid hormone synthesis. Nevertheless, the effects of FOXL2on granulosa cell morphology and the underlying mechanism remain unknown. Using FOXL2 ChIP-seq analysis, we found that FOXL2 target genes were significantly enriched in the actin cytoskeleton-related pathways. We confirmed that FOXL2inhibited the expression of RhoA, a key gene for actin cytoskeleton rearrangement, by binding to TCATCCATCTCT in RhoApromoter region. In addition, FOXL2overexpression in granulosa cells induced the depolymerization of F-actin and disordered the actin filaments, resulting in a slowdown in the expansion of granulosa cells, while FOXL2silencing inhibited F-actin depolymerization and stabilized the actin filaments, thereby accelerating granulosa cell expansion. RhoA/ROCK pathway inhibitor Y-27632 exhibited similar effects to FOXL2overexpression, even reversed the actin polymerization in FOXL2silencing granulosa cells. This study revealed for the first time that FOXL2regulated granulosa cell actin cytoskeleton by RhoA/ROCK pathway, thus affecting granulosa cell expansion. Our findings provide new insights for constructing the regulatory network of FOXL2and propose a potential mechanism for facilitating rapid follicle expansion, thereby laying a foundation for further understanding follicular development.FOXL2regulates actin cytoskeleton rearrangement of granulosa cells (GCs) through RhoA/ROCK pathway, thus affecting GC expansion of pre-ovulatory follicles in chicken.Graphical Abstract

Published
2024
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7. Duck pan‐genome reveals two transposon insertions caused bodyweight enlarging and white plumage phenotype formation during evolution.

Author

Wang, Kejun, Hua, Guoying, Li, Jingyi, Yang, Yu, Zhang, Chenxi, Yang, Lan, Hu, Xiaoyu, Scheben, Armin, Wu, Yanan, Gong, Ping, Zhang, Shuangjie, Fan, Yanfeng, Zeng, Tao, Lu, Lizhi, Gong, Yanzhang, Jiang, Ruirui, Sun, Guirong, Tian, Yadong, Kang, Xiangtao, and Hu, Haifei

Subjects
PAN-genome, QUANTITATIVE genetics, MOLECULAR genetics, PHENOTYPES, FEATHERS, GENE ontology
Abstract

Structural variations (SVs) are a major source of domestication and improvement traits. We present the first duck pan‐genome constructed using five genome assemblies capturing ∼40.98 Mb new sequences. This pan‐genome together with high‐depth sequencing data (∼46.5×) identified 101,041 SVs, of which substantial proportions were derived from transposable element (TE) activity. Many TE‐derived SVs anchoring in a gene body or regulatory region are linked to duck's domestication and improvement. By combining quantitative genetics with molecular experiments, we, for the first time, unraveled a 6945 bp Gypsy insertion as a functional mutation of the major gene IGF2BP1 associated with duck bodyweight. This Gypsy insertion, to our knowledge, explains the largest effect on bodyweight among avian species (27.61% of phenotypic variation). In addition, we also examined another 6634 bp Gypsy insertion in MITF intron, which triggers a novel transcript of MITF, thereby contributing to the development of white plumage. Our findings highlight the importance of using a pan‐genome as a reference in genomics studies and illuminate the impact of transposons in trait formation and livestock breeding. Highlights: We present the first duck pan‐genome constructed using five genome assemblies capturing ∼40.98 Mb new sequences absent from the reference genome.We find a significant portion of the detected structural variants were derived from transposable element (TE) activity. Many of these are located within gene bodies or regulatory regions, potentially linked to duck domestication and enhancement.We used two representative examples to show how TE insertions can lead phenotypic diversity, highlighting IGF2BP1's role in bodyweight and MITF's influence on white plumage in ducks.Notably, the Gypsy insertion in the IGF2BP1 promoter, to our knowledge, explains the largest effect on bodyweight among avian species (27.61% of phenotypic variation). [ABSTRACT FROM AUTHOR]

Published
2024
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10. Duck pan-genome reveals two transposon-derived structural variations caused bodyweight enlarging and white plumage phenotype formation during evolution

Author

Wang, Kejun, primary, Hua, Guoying, additional, Li, Jingyi, additional, Yang, Yu, additional, Zhang, Chenxi, additional, Yang, Lan, additional, Hu, Xiaoyu, additional, Scheben, Armin, additional, Wu, Yanan, additional, Gong, Ping, additional, Zhang, Shuangjie, additional, Fan, Yanfeng, additional, Zeng, Tao, additional, Lu, Lizhi, additional, Gong, Yanzhang, additional, Jiang, Ruirui, additional, Sun, Guirong, additional, Tian, Yadong, additional, Kang, Xiangtao, additional, Hu, Haifei, additional, and Li, Wenting, additional

Published
2023
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11. Assessment of the Endophytic Fungal Composition of Lactobacillus plantarum and Enterococcus faecalis-Fermented Astragalus membranaceus Using Single-Molecule, Real-Time Sequencing Technology

Author

Zhang, Liheng, Li, Xianghui, Song, Xinghui, Bian, Chuanzhou, Kang, Xiangtao, Zhao, Junqiang, Qiao, Hongxing, and Gong, Yanzhang

Subjects
General Veterinary
Abstract

Endophytic fungus represents microorganisms existing within the healthy plant organs, which can significantly influence metabolic product production in plants, a process with great research value and broad prospects for development. To investigate the effect of fermentation with probiotic cultures on the endophytic fungal diversity and composition of Astragalus membranaceus, we used single-molecular, real-time sequencing (Pacific Biosciences) for 18S ribosomal RNA (rRNA) sequencing. The results showed that the endophytic fungi of A. membranaceus mainly belonged to Aspergillus, Penicillium, Cystofilobasidium, Candida, Guehomyces, and Wallemia. Furthermore, the endophytic fungal diversity and abundance of A. membranaceus were more variable after fermentation with Enterococcus faecium and/or Lactobacillus plantarum. Our data lays a solid and comprehensive foundation for further exploration of endophytic fungi from A. membranaceus as potential sources of functional compounds.

Published
2022

21. Phenotype characterization of crossed beaks in Beijing-You chickens based on morphological observation

Author

Shi, Lei, primary, Li, Yunlei, additional, Bai, Hao, additional, Li, Dongli, additional, Wang, Panlin, additional, Jiang, Linlin, additional, Fan, Jing, additional, Ge, Pingzhuang, additional, Ni, Aixin, additional, Wang, Yuanmei, additional, Bian, Shixiong, additional, Zong, Yunhe, additional, Isa, Adamu Mani, additional, Tesfay, Hailai Hagos, additional, Ma, Hui, additional, Gong, Yanzhang, additional, Sun, Yanyan, additional, and Chen, Jilan, additional

Published
2020
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25. Characterization and proteomics of chicken seminal plasma extracellular vesicles.

Author

Luo, Xuliang, Guo, Yan, Huang, Ying, Cheng, Manman, Wu, Xiaohui, and Gong, Yanzhang

Subjects
EXTRACELLULAR vesicles, CHICKENS, CARRIER proteins, TRANSMISSION electron microscopy, SPERM motility
Abstract

In mammals, seminal plasma extracellular vesicles (SPEVs) can regulate sperm motility and capacitation. The characteristics and functions of SPEVs in avians have been rarely reported. In this study, chicken SPEVs were isolated and characterized by transmission and scanning electron microscopy (TEM/SEM) and nanoparticle tracking analysis (NTA); furthermore, seven extracellular vesicle (EVs) marker proteins were detected by Western blot (WB). TEM revealed that chicken SPEVs had a classic bilayer membrane structure. NTA confirmed that the size of SPEVs was 30–250 nm, and concentration ranged from 8.0 E + 11–8.5 E + 11 particles/ml. There were 3073 SPEVs proteins identified by deep sequencing, including 2794 intracellular proteins and 279 extracellular proteins. The overlap rate of proteomes between chicken SPEVs and vesicles reported in the Vesiclepedia database reached 86%, and 360 new proteins that had not been reported by the ExoCarta and Vesiclepedia databases were identified in chicken SPEV proteomes. Gene Ontology (GO) analysis revealed that chicken SPEV proteins were mainly enriched in supplying energy and transporting protein. There were 4 IFT family proteins speculated to play an important role in sperm composition and function. Our data were compared with two previously published studies on the proteomics of chicken seminal plasma (SP) and hen uterine fluid, and some overlapping proteins described in chicken SPEVs had been identified in hen uterine fluid (545) and chicken SP (284). In conclusion, these findings will increase our understanding of the content and composition of proteome in SPEVs and provide new insights into the important role of the SPEV regulation in sperm functions. [ABSTRACT FROM AUTHOR]

Published
2022
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33. Molecular Cloning, Characterization and Expression Analysis of Duck Tyrosinase-Related Protein-1

Author

Feng YanPing, Gong YanZhang, Li ShiJun, Liang ZhenHua, Yu HuaWen, Wang Cui, and Peng XiuLi

Subjects
Signal peptide, Messenger RNA, General Veterinary, Tyrosinase, Biology, Molecular cloning, Molecular biology, law.invention, chemistry.chemical_compound, Open reading frame, chemistry, law, Complementary DNA, Agronomy and Crop Science, DNA, Polymerase chain reaction
Published
2010

38. Genetic variation of 27 microsatellite loci in three Hubei indigenous pig breeds

Author

Y Chen, Gong YanZhang, Peng ZhongZhen, Fan Bin, Li Kui, Zhao ShuHong, and C Moran

Subjects
Genetics, Genetic diversity, Ecology, Zoology, Genetic relationship, Locus (genetics), Biology, Breed, Genetic variation, Microsatellite, Genetic variability, Allele, Ecology, Evolution, Behavior and Systematics, Nature and Landscape Conservation
Abstract

Genetic variability within and among three indigenous pig breeds (Tongcheng Pig, Qingping Pig and Yangxin Pig) in Hubei Province was surveyed, by means of 27 microsatellite loci recommended by ISAG (the International Society for Animal Genetics) and FAO(Food and Agriculture Organization). Genetic heterozygosity of each pig breed, polymorphism information content of each microsatellite locus and genetic distances among these populations were calculated. The results showed that allelic number and frequencies at the same locus obviously differed among the breeds. Some alleles were shared by all breeds, for instance 159bp, 162bp and 164bp in S0155, and 110bp in Sw72, while some other alleles were observed only in a single breed, such as 94bp and 96bp in Sw72 that were found solely in Yangxin Pig, 233bp, 245bp and 249bp in S0288 were in Tongcheng Pig, 249bp and 253bp in Sw90 were in Qingping Pig. Therefore, the common alleles (such as 158bp, 162bp and 164bp in S0155) are likely to be the oldest and the most distinctive for the native pig breeds of China. Geneic heterozygosity of three pig breed is 0.7489, 0.6987 and 0.6273 respectively, indication high genetic diversity. Genetic relationship between Tongcheng Pig and Qingping Pig is closer than their relationship with Yangxin Pig.

Published
1999

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