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Anacroneuria flintorum Froehlich, 2002 Anacroneuria flintorum Froehlich, 2002: 93; Bispo & Froehlich, 2004: 99; De Ribeiro & Froehlich, 2007: 55; Froehlich, 2010: 56; Baldin et al., 2013: 394; Gon��alves et al., 2017: 146. Material examined. BR, SP: Iporanga, Gruta da Tapagem, 15.xi.1991, 1 male; PEI, Rio do Carmo, 19.ii.1992, 1 male; PEI, Rio do Carmo, 16.ii.1993, 2 males; PEI, Rio Po��os Altos, 10.xi.1993, CGF & HP col., 2 males; PETAR, Rio Betari, Bairro da Serra, 20.viii.1996, light trap, ASM col., 1 male; PEI, C��rrego Lajeado, 24.ii.1999, 2 males; PEI, C��rrego Lajeado, 17.ix.1999, 1 male; PEI, Ribeir��o ��gua Comprida, 27.x.1999, 3 males; PEI, Rio do Carmo, 28.x.1999, 1 male; PEI, Ribeir��o ��gua Comprida, 23.xi.1999, 7 males; PEI, Ribeir��o ��gua Comprida, 06.i.2000, 8 males; PEI, Rio Po��os Altos, 01.ii.2000, 2 males; PEI, Rio do Carmo, 25.iii.2000, 1 male; PEI, Ribeir��o ��gua Comprida, 24��17���38���S, 48��25���04���W, 30.x.2002, ASM col., 1 male; PECB, Rio Taquaral, 09.ix.2006, 1 male; PEI, C��rrego Roda D�����gua, 13.xii.2014, light trap, PCB col., 1 female; PEI, Rio do Carmo, bridge, 24��18���15���S, 48��24���31���W, 14.xii.2014, light trap, PCB col., 1 male and 1 female; PEI, C��rrego do Mirante, 15.xii.2014, light trap, PCB col., 1 female; PEI, Rio do Carmo, bridge, 24��18���15���S, 48��24���31���W, 08.ii.2017, light trap, LHA et al. col., 1 male; PEI, Ribeir��o Bocaina, bridge, 24��16���13���S, 48��27���09���W, 12.ii.2017, light trap, LHA et al. col., 1 male; PETAR N��cleo Santana, Riacho Furnas, 24��32���03���S, 48��42���02���W, 14.ii.2017, light trap, LHA et al. col., 2 males; PETAR N��cleo Santana, Rio Roncador, 24��32���00���S, 48��42���06���W, 15.ii.2017, light pan trap, LHA et al. col., 1 male; PECB, Bra��o do Rio Taquaral, 24��03���31���S, 47��59���41���W, 05.ii.2017, D-net, LHA et al. col., 1 nymph; PECB, Ribeir��o de Pedras, 24��03���40���S, 47��59���51���W, 06.ii.2017, D-net, LHA et al. col., 1 nymph; PECJ, C��rrego Campo do Meio 22��41���28���S, 45��28���26���W, 28.i.2009, light trap, 2 males. BR, ES: Capara��, Rio Santa Marta, 18.ii.2016, FFST col., 3 females and 7 nymph; Capara��, Pedra Menina, Base, 14-15.ii.2016, FFST col., 2 females; Capara��, Pedra Menina, Base, 15-16.ii.2016, FFST col., 6 females and 1 male. BR, MG: Araponga, Serra do Brigadeiro, Vale das Luas, 14.i.2014, FFST col. 1 nymph. BR, SC: Chapad��o, 28#12���09���S, 49#23���14���W, 15.ii.2013, light trap, L.C. Pinho, A.P. Amaral, M.C. Novaes col., 1 male; Orleans, Rio da Serra, 28��12���43���S, 49#27���28���W, 16.ii.2013, light trap, L.C. Pinho, A.P. Amaral, M.C. Novaes col., 1 male. Description and association of nymph. Molecular association. We associated adults with nymphs of A. flintorum (including teneral adults) from the states of Esp��rito Santo and S��o Paulo. The COI divergences ranged from 0���0.9% for specimens from the S��o Paulo State and from 0���0.7% from Esp��rito Santo State. Intraspecific divergences ranged from 0���3.9%, probably due the geographic variation. For all species studied, interspecific divergences ranged from 12.4%, between A. flintorum (PLE481a) and A. sp. 2 (PPI 15) to 20.6% between A. flintorum (PLE479a) and A. debilis (Pictet, 1841) (PLE480b). A dendrogram and the matrix with pair divergence between specimens based on COI sequences are presented in Fig. 1 and Table 2. Description of nymph. General color dark brown in dorsal view and light yellow in ventral view. Head dark brown with light yellow areas near post-frontal suture, occiput and frontoclypeal region; M-line complete, with well-defined limits; eyes and ocelli black; ocelli with a translucent layer (Fig. 2A); clypeus dark brown; labrum light yellow; maxillae light yellow with lacinia light brown and galea brown; mandible black with median region dark brown with f5 pointed teeth on distal margin, decreasing in size toward base, but teeth 3 and 4 similar in size (Figs. 3A, 3B and 3C). Pronotum dark brown with thin median line and scattered rugosities light yellow (Fig. 2A). Wing pad light with contour partially dark brown (Fig. 2C). Leg brown, forefemur with a central pale line without setae and with lateral surface covered by sparse bristles, in dorsal view; forefemur anteriorly and posteriorly with higher density of thick bristles, and posteriorly with fringe of hairs; tibia anteriorly with sparse thick bristles, and posteriorly with a row of thick bristles and a well-developed band of hairs (Figure 3D). Abdomen dark brown, almost black, with a drop-shaped light spot in the last segment (Fig. 2B). Cerci brown with thick bristles; segments differ in shape and size from base to apex (Fig. 3E). Thoracic gills (Fig. 2D): ASC 1, AT2, AT3 and PT3. Measurements (n=4): head width 3-5 mm; head length 2-3 mm; antennae length 7-8 mm; pronotum width 3-3.5 mm; pronotum length 1.5- 2 mm; cercal length 9-12 mm; total length 10-15mm. Adult morphological variation. Examination of specimens from different regions of Atlantic Forest (states of Esp��rito Santo, Minas Gerais, Santa Catarina, and S��o Paulo) indicated morphological variation. Forewing length of male adults of A. flintorum range from 10 to 15.2 mm (specimens studied herein and Bispo & Froehlich, 2004; Froehlich, 2010, and Baldin et al., 2013). The dorsal color pattern of head of adult of A. flintorum can be variable, even within the same population (Bispo & Froehlich, 2004). In addition, it is possible to observe that the dark brown color of recently collected specimens can become ochraceous when preserved in 80% and 100% ethanol for an extended period of time (Fig. 4). Therefore, it is difficult to identify the species by color pattern alone. For identification, it is essential to study the male penial armature, which in A. flintorum is elongated, tapering progressively from base to the apex and has hooks well separated from distal vesicles (Froehlich, 2002; Bispo & Froehlich, 2004). However, the keel of penial armature may diverge in specimens from the same population and in different populations. In dorsal view, the keel varies from rounded (male specimens from Rio de Janeiro State), pointed (male specimens from PECJ), to convergent or parallel lines (male specimens from PEI). The specimens collected farther north of the species distribution (e. g. ES, RJ and North of SP) have penial armature more robust than specimens from further south (e.g. South of SP and SC). Teneral adult specimens. Teneral specimens of A. flintorum studied are generally brown in color with pale areas along the body; head and pronotum brown with a pale outline. In these specimens, the pronotum does not have lateral folds at the edges, wings are totally pale and the thoracic segments present remnants of gills (Figure 5D, 5E and 5F). A similar pattern to A. flintorum is also present in A. debilis (Almeida & Duarte, 2017) suggesting that several species may be similar when teneral. Therefore, species described as having pale areas on the head and pronotum and milky wings (e.g. A. petersi Froehlich, 2002) may have been based on teneral specimens, requiring caution for use in identifications. Remarks. The nymph of A. flintorum has a different pattern than the other known Anacroneuria nymphs described from Brazil (Righi-Cavallaro & Lecci, 2010; Avelino-Capistrano et al., 2011a; Novaes et al., 2012; Righi-Cavallaro & Froehlich, 2013; Ribeiro & Gorayeb, 2014; Gon��alves et al., 2017). The color pattern of the head and the drop-shaped light spot in the last abdominal segment are apparently characteristic for this species. In addition to description of the nymph, we present new species records from the states of Minas Gerais and Santa Catarina. Anacroneuria flintorum has been collected from streams from protected areas of the Atlantic Forest (Fig. 6). In general, these streams are pristine and have fast currents and rocky substrates, and undisturbed channels and banks. This suggests that A. flintorum may be a sensitive taxon to environmental disturbances., Published as part of Almeida, Lucas Henrique De, Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro, Paresqui, Rayner Constantino & Bispo, Pit��goras Da Concei����o, 2018, Anacroneuria flintorum Froehlich 2002 (Plecoptera: Perlidae): Notes, distribution, and life stages association using molecular tools, pp. 409-420 in Zootaxa 4370 (4) on pages 411-415, DOI: 10.11646/zootaxa.4370.4.6, http://zenodo.org/record/1146515, {"references":["Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera: Perlidae). Proceedings of the Biological Society of Washington, 115, 75 - 107.","Bispo, P. C. & Froehlich, C. G. (2004) Perlidae (Plecoptera) from Intervales State Park, Sao Paulo State, Southeastern Brazil, with descriptions of new species. Aquatic Insects, 26 (2), 97 - 113. https: // doi. org / 10.1080 / 01650420412331325837","De Ribeiro, V. R. & Froehlich, C. G. (2007) Two new species of Anacroneuria (Plecoptera: Perlidae) from southern Brazil. Zootaxa, 1624, 53 - 57.","Froehlich, C. G. (2010) Anacroneuria (Plecoptera, Perlidae) from the Mantiqueira Mountains, Sao Paulo State, Brazil. Zootaxa, 2365, 55 - 68.","Baldin, C., Bispo, P. C. & Novaes, M. C. (2013) New species and records of Anacroneuria (Plecoptera: Perlidae) from Rio de Janeiro State, Brazil. Zootaxa, 3694 (4), 391 - 397. https: // doi. org / 10.11646 / zootaxa. 3694.4.7","Goncalves M. C., Novaes, M. C. & Salles, F. F. (2017) New species and records of Perlidae (Plecoptera) from Espirito Santo State, Brazil. Zootaxa, 4273 (1), 141 - 150. https: // doi. org / 10.11646 / zootaxa. 4273.1.11","Pictet, F. - J. (1841) Histoire Naturelle Generale et Particuliere des Insectes Nevropteres. Famille des Perlides. J. Kessmann, Geneve, U. S. A. https: // doi. org / 10.5962 / bhl. title. 124172","Almeida, L. H. & Duarte, T. (2017) A new species and records of Anacroneuria (Plecoptera: Perlidae) from the Ecological Station, Wenceslau Guimaraes, State of Bahia, Brazil. Zootaxa, 4247 (4), 480 - 486. https: // doi. org / 10.11646 / zootaxa. 4247.4.9","Righi-Cavallaro, K. O. & Lecci, L. S. (2010) Three new species of Anacroneuria (Plecoptera: Perlidae) from Centre-West and Southeast Brazil. Zootaxa, 2683, 35 - 44.","Avelino-Capistrano, F., Barbosa, L. S. & Almeida, G. L. (2011 a) The nymph of Anacroneuria debilis (Pictet, 1841) (Plecoptera: Perlidae). Zootaxa, 2882, 59 - 63.","Novaes, M. C., Bispo, P. C. & Higuti, J. (2012) A description of the nymph of Anacroneuria ofaye Froehlich (Plecoptera: Perlidae) and a apparatus for rearing Neotropical stonefly species. Zootaxa, 3547, 71 - 77.","Righi-Cavallaro, K. O. & Froehlich, C. G. (2013) The Nymph of of Anacroneuria payagua Froehlich (Plecoptera: Perlidae) from Brazil. Zootaxa, 3635 (5), 579 - 582. https: // doi. org / 10.11646 / zootaxa. 3635.5.7","Ribeiro, J. M. F. & Gorayeb, I. S. (2014) Description of immatures and association with adults of three species of Anacroneuria Klapalek (Plecoptera: Perlidae) of the Brazilian Amazon. Zootaxa, 3881 (1), 17 - 32. https: // doi. org / 10.11646 / zootaxa. 3881.1.2"]}

Kempnyia colossica (Nav��s 1934) Material examined. BRA, ES, Municipality of Santa Teresa, District of Nova Lombardia, S��tio Capitel de Santo Ant��nio, C��rrego Grande, Pinguela, S19��52'16.0" W40��31'43.1", 718 m, D-net, 16.xi.2007, 2 nymphs, FFST, CZNC. Remarks. This is a new record for Esp��rito Santo State, Brazil., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on page 147, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Navas, L. (1934) Familia Perlidos. In: Insectos suramericanos. Revista de la Real Academia de Ciencias de Madrid, 31, pp. 22 - 23 & 160 - 163."]}

Gripopteryx caparao n. sp. Figs. 1���3. Material examined. Holotype male (CZNC): BRA, ES, PNC, Municipality of Espera Feliz, border between Minas Gerais and Esp��rito Santo States, Pedra Menina, S20��37'30.3" W41��49'27.1", 884 m, light trap, 14-15.ii.2016, FFST. Description. General color ochraceous. Head ochraceous centrally darker; parietalia light brown; epicranial line dark; clypeal area light brown with border darker. Antennae light yellow, scape and pedicel darker, flagellum light yellow. Pronotum light yellow with dark border and light rugosities. Mesonotum and metanotum light brown, pleura yellowish. Legs light yellow; femora light yellow; base of tibia with a light brown band, the rest light yellowish, tarsi light yellow. Forewing with several spots, venation brown and membrane light brown. A pterostigmatic crossvein present. Male. Abdominal segments slightly sclerotized. Sternum 9 forms a short, elliptical subgenital plate (Fig. 1). Posterior portion of abdominal tergum 10 flattened (Figs. 1, 3). Paraprocts broad, robust and concave internally with apex truncated (Figs. 1���3); epiproct short, scythe���shaped. Basis of cerci light yellow. Total length, 6.0 mm; width of head, 0.6 mm; length of forewing, 8.6 mm; length of antennae 6.5mm and cerci damaged. Remarks. Gripopteryx caparao is a small-sized species, with the male paraprocts concave as in G. japi Lecci & Froehlich 2011, but those of G. caparao are much more expanded and truncated. The epiproct of G. caparao is smaller than G. japi. Additionally, G. caparao is much smaller and lighter than G. japi. Etymology. This name is reference to Parque Nacional do Capara��, where the species was collected., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, Studies on Gripopterygidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 563-571 in Zootaxa 4291 (3) on page 564, DOI: 10.11646/zootaxa.4291.3.8, http://zenodo.org/record/827728, {"references":["Lecci, L. S. & Froehlich, C. G. (2011) Taxonomic revision of Gripopteryx (Pictet, 1841) (Plecoptera: Gripopterygidae). Zootaxa, 2792, 1 - 21."]}

Macrogynoplax nymphs Material examined. BRA, ES, Municipality of Santa Teresa, District of Nova Lombardia, S��tio Capitel de Santo Ant��nio, C��rrego Grande, ��rea aberta/��rea fechada, S19��52'32.6" W40��31"49.8", D-net, 448 m, 18. ii.2009, 1 nymph, FFST, CZNC. BRA, ES, Municipality of Santa Teresa, RBAR, ER 1, S19��51'14.12" W40��33'38.1", D-net, 08���09. ix.2015, 2 nymphs, FFST, CZNC. BRA, ES, Municipality of Santa Teresa, RBAR ER 5, S19��55'26.2" W40��33'41.71", D-net, 29. iv.2012, 3 nymphs, FFST, CZNC. Remarks. The nymphs of Macrogynoplax from this region are undescribed and specific identity is not currently possible., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on pages 148-149, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867

Tupiperla robusta Froehlich 1998 Material examined: BRA, ES, PNC, Municipality of Espera Feliz, border between Minas Gerais and Esp��rito Santo States, Pedra Menina, S 20��37'30.3" W 41��49'27.1", 884 m, light trap, 15.ii.2016, 1 male, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Tecnotruta, Rio Cal��ado, S20��27'58.3" W41��43'49.0", 1057 m, 18.ii.2016, 1 male, FFST, CZNC., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, Studies on Gripopterygidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 563-571 in Zootaxa 4291 (3) on page 570, DOI: 10.11646/zootaxa.4291.3.8, http://zenodo.org/record/827728, {"references":["Froehlich, C. G. (1998) Seven new species of Tupiperla (Plecoptera: Gripopterygidae) from Brazil, with a revision of the genus. Studies on Neotropical Fauna and Environment, 33, 19 - 36."]}

Paragripopteryx. intervalensis Bispo & Lecci 2011 Figs. 7���9. Material examined. BRA, ES, Municipality of Santa Teresa, RBAR, stream along the road, close to small waterfall site S19��53'20.6" W40��32'41.5", 803 m, light trap, 08-09.ix.2015, 1 male and 1 female, FFST, CZNC. Description of Female. General color brown. Head brown, centrally darker, frons and epicranial line dark brown. Antennae brown, scape and pedicel of antennae dark brown, flagellum brown. Pronotum brown with margins and rugosities darker. Mesonotum and metanotum brown, pleura lighter. Legs brown; distal part of femur, tibia and tarsus darker. Forewing and venation brown. Abdominal segments 2���7 membranous with a small spots sclerotized. Sternum 8 dark brown, sclerotized, square-shaped, relatively long (Figs. 7���9). Measurements: total length: 8.0 mm; forewing length: 7.0 mm; antennae length: 7.1 mm; cerci damaged. Remarks. This species was described from one male from Parque Estadual de Intervales (Bispo & Lecci, 2011). The specimens studied here are new records for Esp��rito Santo State, expanding the distribution into southeastern Brazil., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, Studies on Gripopterygidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 563-571 in Zootaxa 4291 (3) on page 568, DOI: 10.11646/zootaxa.4291.3.8, http://zenodo.org/record/827728, {"references":["Bispo, P. C. & Lecci, L. S. (2011) Gripopterygidae (Plecoptera) from Paranapiacaba Mountains, southeastern Brazil. Annales de Limnologie-International Journal of Limnology, 47 (4), 373 - 385."]}

Anacroneuria piti sp. n. Figs. 2A���2 D (male) and Figs. 3A���3 F (nymph). Material examined. Holotype male (CZNC): Brazil (BRA), Esp��rito Santo State (ES), Alegre, Rio Norte, reared, nymph collected 07.viii.2004, emerged 08.viii.2004. Description. General color yellowish. Head yellowish with M-line not distinct, parts of clypeal area yellowish and frons light brown (Fig. 2A); lappets brown; clypeal area setose; post frontal line not distinct; scape and pedicel light brown; flagellomeres light brown; palpi light brown. Pronotum light brown with lighter rugosities (Fig. 2A). Legs yellowish to light brown, tibia and tarsi darker. Wing membrane light brown with veins darker. Cerci light brown. Male: Forewing length 11mm (n=1). Penial armature (Figs. 2 B���2D), in ventral view, gradually tapering from medially to apex and terminating in an indistinct small aperture (Fig. 2 C), with a pair of small distal membranous vesicles (Figs. 2 C���2D); in lateral view, pronounced keel apparent (Fig. 2 D), distinct in dorsal view appearing as two parallels bars (Fig. 2 B); hooks relatively short and flattened, little curved and with acute apices (Fig. 2 C). Nymph: General color brownish. Head brown with lighter areas near post-frontal suture and in frontoclypeal region (Fig. 3A); M-line complete, distinct; eyes black and ocelli black with a darker stain medially. Pronotum brownish with median line and sparse areas yellowish (Fig. 3A). Mandibles, maxillae, and labium as in Figs. 3 B, 3C, 3D. Forefemur with lateral surface covered by scattered bristles (Fig. 3 E); dorsally and ventrally with higher density of thick bristles; dorsally with translucent fringe of hairs. Tibia ventrally with thin and sparse translucent bristles, and dorsally with a row of thick bristles in the border and a well-developed band of hairs. Cerci brown with thick bristles; segments differ in shape and size from base to apex (Fig. 3 E). Thoracic gills: ASC 1, PSC1, AT2, AT3 and PT3 (sensu Shepard & Stewart 1983; Stewart & Stark 2002). Measurements (n=7): head width 2.9��� 3.3 mm; head length 1.9���2.6 mm; antennae length 4.0���5.0mm; pronotum width 2.5���3 mm; pronotum length 1.5���2 mm; cercal length 6.5���7.5 mm; total length 7.9���9 mm. Remarks. The color of the adult is similar to many species of the genus. The penial armature of A. pitii in ventral view (Fig. 2 C) and lateral view (Fig. 2 D) is most similar to A. itatiaiensis Baldin et. al 2013 also known from southeastern Brazil, but differs in the size of membranous vesicles and apex. In A. pitii, the vesicles are smaller and are more medially than in A. itatiaiensis. The penial armature (Figs. 2 B���2D) of A. pitii is more robust basally than A. itatiaiensis. In lateral view (Fig. 2 D), the apex of A. pitii is thinner than A. itatiaiensis; the hooks are more robust in A. piti. Furthermore the color of A. pitii is lighter than A. itatiaiensis. Etymology. The patronym honors Pit��goras da Concei����o Bispo, a Brazilian Plecoptera specialist., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on pages 143-145, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Shepard, W. D. & Stewart, K. W. (1983) Comparative study of nymphal gills in North American stonefly genera and a new, proposed paradigm of Plecoptera gill evolution. Miscellaneous Publication Entomological Society of America, 13, 1 - 57.","Stewart, K. W. & Stark, K. W. (2002) Nymphs of North American Stonefly Genera (Plecoptera). Caddis Press, Columbus, Ohio, 510 pp.","Baldin, C., Bispo, P. C. & Novaes, M. C. (2013) New species and records of Anacroneuria (Plecoptera: Perlidae) from Rio de Janeiro State, Brazil. Zootaxa, 3694 (4), 391 - 397."]}

Anacroneuria rotunda sp. n. Figs. 1A���1 E. Material examined. Holotype male (CZNC): Brazil (BRA), Esp��rito Santo State (ES), Alegre, S20��37'11,4" W41��37'35.4", 04���05.iv.2016, light trap, FFST. Paratypes (CZNC): 12 males: BR, ES, Alegre, S20��37'11,4" W41��37'35.4", 04���05.iv.2016, light trap, FFST. Description. General color dark brown, almost black. Head brown with M-line distinct, areas of clypeus brown, frons orange (Fig. 1A); lappets black; clypeal area setose; post frontal line U-shaped; scape and pedicel dark brown; flagellum dark brown; palpi brownish. Pronotum dark brown almost black with lighter rugosities (Fig. 1A). Legs dark brown to black, tibia darker and tarsi lighter. Wing membrane brown, veins dark brown. Cerci dark brown almost black. Male: Forewing length 11.7���12 mm (n=13). Hammer truncate, wider than long (Fig. 1 B). Penial armature with a pair of medial distal membranous vesicles (Figs. 1 C���1E) and apex bottleneck-shaped, opening relatively large; in lateral view, keel convex apically (Fig. 1 E), dorsal view, U-shaped (Fig. 1 C); hooks relatively short and flattened, little curved and with acute apices (Fig. 1 D). Remarks. The dark color of this species is uncommon in regional species. In dorsal view (Fig. 1 C) and lateral view (Fig. 1 D), the penial armature resembles those of A. singela Duarte & Lecci 2016 described from northeastern Brazil. In this species, however, the keel is not convex apically as in the new species. Additionally, A. rotunda is smaller and darker than A. singela. Etymology. The specific epithet makes reference to the bulged shape of the keel in dorsal and lateral views. The word rotunda derives from the Latin "rotundus," round, spherical or, orbicular., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on page 142, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Duarte, T. & Lecci, L. S. (2016). New species and records of Anacroneuria (Plecoptera: Perlidae) from the northeastern semiarid region of Brazil. Zootaxa, 4079 (2), 291 - 300."]}

Kempnyia neotropica (Jacobson & Bianchi 1905) Material examined. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Rio S��o Domingos, Cachoeira da Farofa, S20��28'19.5" W41��49'41.7", 1972 m, D-net, 3 nymphs in 23.iv.2008, 3 nymphs in 20.iii.2009, 1 nymph in 26.iii.2009, 1 nymph in 02.iv.2011, 4 nymphs in 23.iv.2008, FFST, CZNC. BRA, ES, PNC, Municipality of Espera Feliz, border between Minas Gerais and Esp��rito Santo States, Pedra Menina, S20��37'30.3" W41��49'27.1", 884 m, light trap, 15.x.2011, 2 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Tecnotruta, Fazenda Sonho Meu, S20��28'08" W41��43'22.5", 959 m, 21.iv.2008, D-net, 1 nymph, FFST, CZNC. BRA, ES, Municipality of Santa Teresa, District of Nova Lombardia, S��tio Capitel de Santo Ant��nio, C��rrego Grande, Pinguela, S19��52'16.0" W40��31" 43.1", 718 m, D-net, 19.i.2008, 2 nymphs, FFST, CZNC. BRAZIL, ES, Municipality of Santa Teresa, District of Nova Lombardia, S��tio Capitel de Santo Ant��nio, C��rrego Grande, Cachoeira Grande, S19��52'30.8" W40��31" 56.6", D-net, 768 m, 20.i.2008, 1 nymph, FFST, CZNC. BRAZIL, ES, Municipality of Santa Teresa, District of Nova Lombardia, S��tio Capitel de Santo Ant��nio, C��rrego Grande, ��rea aberta/��rea fechada, S19��52'32.6" W40��31" 49.8", D-net, 448 m, 20.i.2008, 7 nymphs, FFST, CZNC., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on page 147, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Jacobson, G. G. & Bianchi, V. L. (1905) Orthoptera and Pseudoneuroptera from the Russian Empire and Neighboring Lands. Izdanie A. F. Devriena, Leningrad, 952 pp. [in Russian]"]}

Gripopteryx reticulata (Brauer 1866) Material examined: BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Vale Verde, S 20��25'11.6'' 41�� 50' 45.7'' W, 1612 m, light trap, 23. iii.2009, 1 male and 3 females, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Brejo, Macieira, S 20��28'51.8'' 41�� 49' 43.5'' W, 1857 m, light trap, 25-27. iii.2009, 1 female, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Rio S��o Domingos, Sete Pil��es, S 20��28'57.1'' 41�� 49' 50.4'' W, 1850 m, D-net and light trap, 15-16. ii.2016, 1 nymph, 1 male and 1 female; BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Rio S��o Domingos, Macieira, S 20��28'52.6'' 41�� 49' 44.6'' W, 1854 m, light trap, 26. iii.2016, 1 male, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Jequitib��, border between Minas Gerais and Esp��rito Santo States, Cachoeira Bonita, S20��24'27.7" W41��50'24.1", 1329 m, D-net, 30. v.2011, 1 nymph, FFST, CZNC. BRA, ES, PNC, Municipality of Espera Feliz, border between Minas Gerais and Esp��rito Santo States, Pedra Menina, S 20��37'30.3" W 41��49'27.1", 884 m, D-net, 16. ii.2016, 2 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Santa Marta, Rio Santa Marta, S20��29'51.3" W41��43'45.9", 935 m, D-net, 1. vi.2011, 1 nymph, FFST, CZNC. BRA, ES, Municipality of Santa Teresa, RBAR, S19��53'20.6" W40��32'41.5", 803 m, D-net, 1. v.2013, 2 nymphs, FFST, CZNC. Remarks. This is a new record for Esp��rito Santo State, BRA., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, Studies on Gripopterygidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 563-571 in Zootaxa 4291 (3) on page 568, DOI: 10.11646/zootaxa.4291.3.8, http://zenodo.org/record/827728, {"references":["Brauer, F. (1866) Familie Perlidae. Neuropteren. Reise der Osterreichischen Fregatte Novara um die Erde in den Jahren 1857, 1858, 1859, Zoologischer Theil, 2, Band (1: A), 4, 51 - 52."]}

Kempnyia obtusa Klap��lek 1916 Material examined. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Rio S��o Domingos, Cachoeira da Farofa, S20��28'19.5" W41��49'41.7", 1972 m, D-net, 1 nymph in 23.iv.2008, 5 nymphs in 02.vi.2011, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Rio S��o Domingos, Sete Pil��es, S 20�� 28' 57.1 ' W 41�� 49' 50.4 ', 1850 m, D-net, 22.iv.2008, 1 nymph, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Vale Encantado, S 20��24'38.7'' W 41�� 50' 3.6'', 1976 m, D-net and light trap, 30.v.2011, 3 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Rio S��o Domingos, Macieira, S 20��28'52.6'' 41�� 49' 44.6'' W, 1854 m, D-net, 23.iv.2008, 2 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, Vale Encantado, S 20��24'38.7'' W 41�� 50' 3.6'', 1976 m, D-net, 22.iv.2008, 3 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Alto Jequitib��, border between Minas Gerais and Esp��rito Santo States, Cachoeira Bonita, S20��24'27.7" W41��50'24.1", 1329 m, D-net, 30.v.2011, D-net, 2 nymphs, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Santa Marta, Rio Santa Marta, S20��29'51.3" W41��43'45.9", 935 m, D-net and light trap, 3 nymphs in 31.v.2011, 1 male in 16���18.ii.2016, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Rio entre Pedra Roxa e Santa Marta, S20��28'43.0" W41��42'15.8", 833 m, 31.v.2011, D-net, 1 nymph, 20.iv.2008, FFST, CZNC. BRAZIL, ES, Municipality of Santa Teresa, District of Nova Lombardia, S��tio Capitel de Santo Ant��nio, C��rrego Grande, ��rea aberta/��rea fechada, S19��52'32.6" W40��31" 49.8", D-net, 448 m, 25.x.2008, 1 nymph, FFST, CZNC. Remarks. This is a new record from Esp��rito Santo State, Brazil., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on page 148, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Klapalek, F. (1916) Subfamilia Acroneuriinae Klp. Casopis Ceske Spolecnosti Entomologicke, 13, 45 - 84."]}

Anacroneuria subcostalis Klap��lek 1921 Material examined. BRA, ES, Municipality of Santa Teresa, RBAR, Cachoeirinha da Estrada, S19��53'20.6" W40��32'41.5", 803 m, light trap, 20.v.2016, 1 male and 3 females, FFST, CZNC., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on page 147, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Klapalek, F. (1921) Plecopteres nouveaux. Troisieme partie. Annales de la Societe Entomologique de Belgique, 61, 320 - 327."]}

Gripopteryx pilosa Froehlich 1990 Material examined: BRA, ES, PNC, Municipality of Alto Capara��, border between Minas Gerais and Esp��rito Santo States, stream near road to Casa Queimada, S 20��29'26.0'' 41�� 49' 17.5'' W, 2250 m, D-net, 25.iii.2009, 1 nymph, FFST. CZNC. BRA, ES, PNC, Municipality of Espera Feliz, border between Minas Gerais and Esp��rito Santo States, Pedra Menina, S 20��37'30.3" W 41��49'27.1", 884 m, D-net, 16.ii.2016, 1 nymph, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Rio Cal��ado, S 20��27'58.3" W 41��43'49.0", 1057 m, D-net, 18.ii.2016, 1 nymph, FFST, CZNC. Remarks. This is a new record for Esp��rito Santo State, BRA., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, Studies on Gripopterygidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 563-571 in Zootaxa 4291 (3) on pages 566-568, DOI: 10.11646/zootaxa.4291.3.8, http://zenodo.org/record/827728, {"references":["Froehlich, C. G. (1990) Brazilian Plecoptera 6. Gripopteryx from Campos do Jordao, State of Sao Paulo (Gripopterygidae). Studies on Neotropical Fauna and Environment, 25 (4), 235 - 247."]}

Anacroneuria flintorum Froehlich 2002 Material examined. BRA, ES, Municipality of Barra de S��o Francisco, Itauninha, Cachoeira de Itauninha, S18��49'55.1" W40��54'39.9", 207 m, light trap, 21. i.2011, 1 male, FFST, CZNC. BRA, ES, Municipality of Domingos Martins, Rio Jucu, S20��18'59.5" W40��39'24.4", 469 m, light trap, 08. v.2015, 1 male, FFST, CZNC. BRA, ES, PNC, Municipality of Espera Feliz, border between Minas Gerais and Esp��rito Santo States, Pedra Menina, S20��37'30.3" W41��49'27.1", 884 m, light trap, 14���15. ii.2016, 1 male and 8 females, FFST, CZNC. BRA, ES, PNC, Municipality of Ibitirama, Santa Marta, Rio Santa Marta, S20��29'51.3" W41��43'45.9", 935 m, light trap and D-net, 18. ii.2016, 3 females, 7 nymphs, FFST, CZNC. Remarks. This is a new record for Esp��rito Santo State, Brazil., Published as part of Gon��alves, Ma��sa De Carvalho, Novaes, Marcos Carneiro & Salles, Frederico Falc��o, 2017, New species and records of Perlidae (Plecoptera) from Esp��rito Santo State, Brazil, pp. 141-150 in Zootaxa 4273 (1) on page 146, DOI: 10.11646/zootaxa.4273.1.11, http://zenodo.org/record/801867, {"references":["Froehlich, C. G. (2002) Anacroneuria mainly from southern Brazil and northeastern Argentina (Plecoptera, Perlidae). Proceedings of the Biological Society of Washington, 115 (1), 75 - 107."]}

Anacroneuria ruschii n. sp. Figure 2 (A���G) Material examined. Holotype: male: Brazil, Esp��rito Santo State, Santa Teresa, S19o54���53.42������ W40o33���40.36������, 08���09.ix.2015, light trap, FFST (CZNC). Paratype; 1 male: Santa Teresa, S19o54���53.42������ W40o33���40.36������, 08��� 09.ix.2015, light trap, FFST (CZNC); Santa Teresa, S19o54���53.42������ W40o33���40.36������, 08���09.ix.2015, light trap, 2 females, FFST (CZNC). Description. General color brown. Head brown with M-line, portions of clypeus and frons orange (Fig. 2 A); lappets dark brown; clypeal area setose; post frontal line V-shaped; scape and pedicel dark brown; flagellum brown to dark brown; palpi brownish. Pronotum dark brown with darker rugosities (Fig. 2 A). Legs brown to dark brown, tibia and tarsi darker. Wing membrane brown, veins dark brown. Cerci brown. Male: Forewing length 8.1���8.3 mm. Hammer thin, longer than wide (Fig. 2 B). Penial armature with a pair of medium distal membranous vesicles (Fig. 2 C���D) and apex bottleneck-shaped with an opening; in lateral view, keel with a pointed protrusion between two flaps (Fig. 2 C), in dorsal view, thimble-shaped (Fig. 2 D); hooks relatively large, regularly curved and with apex acute (Fig. 2 C). Female: Forewing length 16.2���16.4 mm. Subgenital plate light brown 4-lobed (Fig. 2 F) with a medial notch deeper. Egg: 0.225 mm x 0.145 mm (Fig. 2 G). Remarks. The overall color of this species is darker than typical species of the genus. In dorsal (Fig. 2 D) and lateral (Fig. 2 E) views, the penial armature resembles those of A. debilis and A. lencoensis Righi-Cavallaro et al.. 2013. However, in A. ruschii, the apex of penial armature and the apex of keel in lateral view are thinner. Furthermore, the vesicles in ventral view are smaller in A. ruschii. The hammer of A. ruschii is relatively acute and thin differing of those of A. debilis and A. lencoensis. The body size of A. ruschii is smaller than A. debilis but similar to that of A. lencoensis. Etymology. The patronym honors Augusto Ruschi, a notable Brazilian naturalist who has spent most of his life devoted to the conservation of natural resources of Esp��rito Santo State, Brazil., Published as part of Novaes, Marcos Carneiro, Bispo, Pit��goras Da Concei����o & Gon��alves, Ma��sa De Carvalho, 2016, A new species of Anacroneuria Klap��lek 1909 (Plecoptera: Perlidae) from Esp��rito Santo State, southeastern Brazil and comments on additional species in Zootaxa 4208 (1), DOI: 10.5281/zenodo.202045