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A considerable increase in endocrine abnormalities has been reported over the last few decades worldwide. A growing exposure to endocrine-disrupting chemicals (EDCs) can be one of the causes of endocrine disorders in populations, and these disorders are not only restricted to the metabolic hormone system but can also cause abnormal functions. Thyroid hormone (TH) disruption is defined as an abnormal change in TH production, transport, function, or metabolism, which results in some degree of impairment in body homeostasis. Many EDCs, including organotin compounds (OTCs), are environmental contaminants that are commonly found in antifouling paints used on ships and in several other industrial procedures. OTCs are obesogenic and can disrupt TH metabolism; however, abnormalities in thyroid function resulting from OTC exposure are less well understood. OTCs, one of the most prevalent EDCs that are encountered on a daily basis, modulate the thyroid axis. In most toxicology studies, it has been reported that OTCs might contribute to hypothyroidism.

Submitted by BIANCA MARIANI GONÇALVES (bia.mariani@yahoo.com.br) on 2018-02-23T04:26:01Z No. of bitstreams: 1 VERSÃO PÓS DEFESA - 22.02.pdf: 2194277 bytes, checksum: 5b59ce8ea0c427305edc63f95e91d00f (MD5) Rejected by Maria Lucia Martins Frederico null (mlucia@fca.unesp.br), reason: Prezada Inserir em seu arquivo a ficha catalográfica - solicite em : fichacatalografica@fca.unesp.br Inserir nos agradecimentos - Agradecimento ao órgão financiador - CNPQ on 2018-02-23T11:14:10Z (GMT) Submitted by BIANCA MARIANI GONÇALVES (bia.mariani@yahoo.com.br) on 2018-02-23T19:37:18Z No. of bitstreams: 1 VERSÃO PÓS DEFESA - 23.02.pdf: 2223850 bytes, checksum: 99d0c3b86baf39d8a2b0bbf2f6a38421 (MD5) Approved for entry into archive by Maria Lucia Martins Frederico null (mlucia@fca.unesp.br) on 2018-02-26T12:22:25Z (GMT) No. of bitstreams: 1 goncalves_bm_me_botfca_par.pdf: 504384 bytes, checksum: e44c795c941c759f9229a26e917a455a (MD5) Made available in DSpace on 2018-02-26T12:22:25Z (GMT). No. of bitstreams: 1 goncalves_bm_me_botfca_par.pdf: 504384 bytes, checksum: e44c795c941c759f9229a26e917a455a (MD5) Previous issue date: 2018-02-16 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) O diabetes mellitus (DM) é uma das doenças que mais tem aumentado sua incidência na última década. As células-tronco mesenquimais derivadas de tecido adiposo (CTMs-TA) possuem características imunomoduladoras e reparadoras, sendo capazes de reverter efeitos sistêmicos da doença. Sendo assim, nosso trabalho teve como objetivo avaliar o impacto do transplante de CTMs-TA pelas vias intravenosa, intrapancreática e intra-capsular renal em ratos diabéticos e comparar a que melhor exerce efeito regenerador. Para o estudo, utilizamos 45 ratos, wistar, fêmeas, divididos em 5 grupos: GCS – controle sadio; GCD – controle diabético; GIV – diabético, via intravenosa; GIP – diabético, via intrapancreática; GIR – diabético, via cápsula renal. Para a indução, utilizamos uma única aplicação intraperitoneal (i.p) de Aloxana (120mg/kg), e após 3 dias, os animais foram submetidos ao transplante com as CTMs-TA (2x106cel/animal). Após 15 dias do transplante, os animais foram eutanasiados. O transplante de CTMs-TA pela cápsula renal foi capaz de reverter a hiperglicemia, além de restaurar o diâmetro das ilhotas e restabelecer a função das massa β-pancreática. Concluímos que a cápsula renal foi a que apresentou melhores resultados na regeneração morfofuncional do pâncreas diabético. As CTMs-TA utilizadas neste estudo foram capazes de exercer um importante efeito parácrino, podendo ser utilizadas como alternativa para a melhoria da qualidade de vida de indivíduos diabéticos. Diabetes mellitus is one of the diseases that has increased your incidence in the last decade. Adipose-Derived Mesenchymal Stem Cells (ATMSCs) have immunomodulatory and regenerate characteristics, thus are capable of reverse the damages of the disease. Therefore, our study aimed to evaluate the impact of ATMSCs transplantation by the intravenous, intrapancreatic and intra-capsular renal routes in diabetic rats and detect the best route to promote regenerative effects. We used 45 female wistar rats, divided into 5 groups: GCS – healthy control; GCD - diabetic control; GIV - diabetic, intravenous; GIP - diabetic, intrapancreatic route; GIR - diabetic, via renal capsule. For induction, we used a single intraperitoneal application (i.p) of Alloxan (120mg / kg) and after 3 days, the animals were transplanted with the ATMSCs (2x106/ animal). After 15 days of transplantation, the animals were euthanized. The transplantation of ATMSCs by the renal capsule were able to reverse hyperglycemia, besides restoring the islet diameter and restoring the function of β-pancreatic mass. We conclude that the renal capsule presented the best results in the morphofunctional regeneration of the diabetic pancreas. The stem cells used in this study were able to exert an important paracrine effect,and could be used as an alternative to improve the quality of life on diabetic patients. CNPQ: 134517/2016-4