1. Unraveling the antifungal composition of bitter orange decoction against the melon pathogen Fusarium jinanense.
- Author
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de Freitas MD, Dantas Lima Junior R, Freitas da Silva FE, Inokuti EM, Hansen Oster A, Zampieri D, Souza Lima C, Pacheco Fill T, and Gomes de Lemos TL
- Subjects
- Cucurbitaceae chemistry, Cucurbitaceae microbiology, Antifungal Agents pharmacology, Antifungal Agents chemistry, Tandem Mass Spectrometry, Solid Phase Extraction, Microbial Sensitivity Tests, Fusarium drug effects, Plant Diseases microbiology, Plant Extracts pharmacology, Plant Extracts chemistry, Citrus chemistry, Citrus microbiology
- Abstract
Bitter orange (Citrus aurantium) is an important source of essential oils with high antimicrobial activities, however the composition and antifungal potential of the decoction peels is little explored. This study assessed the peel decoction's chemical profile at the secondary metabolism level and its antifungal activity against the melon phytopathogen Fusarium jinanense. The decoction's antifungal potential was investigated using a bioassay-guided fractionation approach based on Solid-Phase Extraction (SPE) and LC-HRMS/MS analysis. Coumarins and flavones were the most abundant classes of compounds in the high-value fractions responsible for up to 61% of the mycelial inhibition of F. jinanense. Overall, this study has presented for the first time the chemical composition, the antifungal potential of the decoction of C. aurantium peels and the compounds associated with these results. This strategy can guide the exploration of under-explored food sources and add value to compounds or fractions enriched with bioactive compounds., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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