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26 results on '"Golovkin, Alexey S."'

2. Immunological Profile and Markers of Endothelial Dysfunction in Elderly Patients with Cognitive Impairments

Author

Goncharov, Nikolay V., primary, Popova, Polina I., additional, Kudryavtsev, Igor V., additional, Golovkin, Alexey S., additional, Savitskaya, Irina V., additional, Avdonin, Piotr P., additional, Korf, Ekaterina A., additional, Voitenko, Natalia G., additional, Belinskaia, Daria A., additional, Serebryakova, Maria K., additional, Matveeva, Natalia V., additional, Gerlakh, Natalia O., additional, Anikievich, Natalia E., additional, Gubatenko, Marina A., additional, Dobrylko, Irina A., additional, Trulioff, Andrey S., additional, Aquino, Arthur D., additional, Jenkins, Richard O., additional, and Avdonin, Pavel V., additional

Published
2024
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3. Chitosan Composites with Bacterial Cellulose Nanofibers Doped with Nanosized Cerium Oxide: Characterization and Cytocompatibility Evaluation

Author

Petrova, Valentina A., primary, Gofman, Iosif V., additional, Dubashynskaya, Natallia V., additional, Golovkin, Alexey S., additional, Mishanin, Alexander I., additional, Ivan’kova, Elena M., additional, Romanov, Dmitry P., additional, Khripunov, Albert K., additional, Vlasova, Elena N., additional, Migunova, Alexandra V., additional, Baranchikov, Alexander E., additional, Ivanov, Vladimir K., additional, Yakimansky, Alexander V., additional, and Skorik, Yury A., additional

Published
2023
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4. Modulation of Notch Signaling at Early Stages of Differentiation of Human Induced Pluripotent Stem Cells to Dopaminergic Neurons

Author

Katolikova, Nataliia V., primary, Khudiakov, Aleksandr A., additional, Shafranskaya, Daria D., additional, Prjibelski, Andrey D., additional, Masharskiy, Alexey E., additional, Mor, Mikael S., additional, Golovkin, Alexey S., additional, Zaytseva, Anastasia K., additional, Neganova, Irina E., additional, Efimova, Evgeniya V., additional, Gainetdinov, Raul R., additional, and Malashicheva, Anna B., additional

Published
2023
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6. Bacterial Cellulose Composites with Polysaccharides Filled with Nanosized Cerium Oxide: Characterization and Cytocompatibility Assessment

Author

Petrova, Valentina A., primary, Gofman, Iosif V., additional, Golovkin, Alexey S., additional, Mishanin, Alexander I., additional, Dubashynskaya, Natallia V., additional, Khripunov, Albert K., additional, Ivan’kova, Elena M., additional, Vlasova, Elena N., additional, Nikolaeva, Alexandra L., additional, Baranchikov, Alexander E., additional, Skorik, Yury A., additional, Yakimansky, Alexander V., additional, and Ivanov, Vladimir K., additional

Published
2022
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8. Effect of Cholecalciferol Supplementation on the Clinical Features and Inflammatory Markers in Hospitalized COVID-19 Patients: A Randomized, Open-Label, Single-Center Study

Author

Karonova, Tatiana L., primary, Golovatyuk, Ksenia A., additional, Kudryavtsev, Igor V., additional, Chernikova, Alena T., additional, Mikhaylova, Arina A., additional, Aquino, Arthur D., additional, Lagutina, Daria I., additional, Zaikova, Ekaterina K., additional, Kalinina, Olga V., additional, Golovkin, Alexey S., additional, Grant, William B., additional, and Shlyakhto, Evgeny V., additional

Published
2022
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9. Vitamin D Status and Immune Response in Hospitalized Patients with Moderate and Severe COVID-19

Author

Karonova, Tatiana L., primary, Kudryavtsev, Igor V., additional, Golovatyuk, Ksenia A., additional, Aquino, Arthur D., additional, Kalinina, Olga V., additional, Chernikova, Alena T., additional, Zaikova, Ekaterina K., additional, Lebedev, Denis A., additional, Bykova, Ekaterina S., additional, Golovkin, Alexey S., additional, and Shlyakhto, Evgeny V., additional

Published
2022
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15. In vitro toxicity of FemOn, FemOn-SiO2 composite, and SiO2-FemOn core-shell magnetic nanoparticles

Author

Toropova, Yana G, Golovkin, Alexey S, Malashicheva, Anna B, Korolev, Dmitry V, Gorshkov, Andrey N, Gareev, Kamil G, Afonin, Michael V, and Galagudza, Michael M

Subjects
Cell Death, Cell Survival, iron oxide nanoparticles, Apoptosis, Silicon Dioxide, Nanocomposites, silica nanoflakes, composite nanoparticles, silica coating, Human Umbilical Vein Endothelial Cells, cytotoxicity, Humans, Magnetite Nanoparticles, Cell Shape, Original Research
Abstract

Over the last decade, magnetic iron oxide nanoparticles (IONPs) have drawn much attention for their potential biomedical applications. However, serious in vitro and in vivo safety concerns continue to exist. In this study, the effects of uncoated, FemOn-SiO2 composite flake-like, and SiO2-FemOn core-shell IONPs on cell viability, function, and morphology were tested 48 h postincubation in human umbilical vein endothelial cell culture. Cell viability and apoptosis/necrosis rate were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and annexin V-phycoerythrin kit, respectively. Cell morphology was evaluated using bright-field microscopy and forward and lateral light scattering profiles obtained with flow cytometry analysis. All tested IONP types were used at three different doses, that is, 0.7, 7.0, and 70.0 μg. Dose-dependent changes in cell morphology, viability, and apoptosis rate were shown. At higher doses, all types of IONPs caused formation of binucleated cells suggesting impaired cytokinesis. FemOn-SiO2 composite flake-like and SiO2-FemOn core-shell IONPs were characterized by similar profile of cytotoxicity, whereas bare IONPs were shown to be less toxic. The presence of either silica core or silica nanoflakes in composite IONPs can promote cytotoxic effects.

Published
2017

17. Modification of the Ceramic Implant Surfaces from Zirconia by the Magnetron Sputtering of Different Calcium Phosphate Targets: A Comparative Study

Author

Kozelskaya, Anna I., primary, Bolbasov, Evgeny N., additional, Golovkin, Alexey S., additional, Mishanin, Alexander I., additional, Viknianshchuk, Alice N., additional, Shesterikov, Evgeny V., additional, Ashrafov, Аndrey, additional, Novikov, Vadim A., additional, Fedotkin, Alexander Y., additional, Khlusov, Igor A., additional, and Tverdokhlebov, Sergey I., additional

Published
2018
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19. Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions

Author

Kutikhin, Anton G., primary, Velikanova, Elena A., additional, Mukhamadiyarov, Rinat A., additional, Glushkova, Tatiana V., additional, Borisov, Vadim V., additional, Matveeva, Vera G., additional, Antonova, Larisa V., additional, Filip’ev, Dmitriy E., additional, Golovkin, Alexey S., additional, Shishkova, Daria K., additional, Burago, Andrey Yu., additional, Frolov, Alexey V., additional, Dolgov, Viktor Yu., additional, Efimova, Olga S., additional, Popova, Anna N., additional, Malysheva, Valentina Yu., additional, Vladimirov, Alexandr A., additional, Sozinov, Sergey A., additional, Ismagilov, Zinfer R., additional, Russakov, Dmitriy M., additional, Lomzov, Alexander A., additional, Pyshnyi, Dmitriy V., additional, Gutakovsky, Anton K., additional, Zhivodkov, Yuriy A., additional, Demidov, Evgeniy A., additional, Peltek, Sergey E., additional, Dolganyuk, Viatcheslav F., additional, Babich, Olga O., additional, Grigoriev, Evgeniy V., additional, Brusina, Elena B., additional, Barbarash, Olga L., additional, and Yuzhalin, Arseniy E., additional

Published
2016
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23. Calcifying nanoparticles: one face of distinct entities?

Author

Kutikhin, Anton G., primary, Yuzhalin, Arseniy E., additional, Borisov, Vadim V., additional, Velikanova, Elena A., additional, Frolov, Alexey V., additional, Sakharova, Vera M., additional, Brusina, Elena B., additional, and Golovkin, Alexey S., additional

Published
2014
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24. Electrospun Composites of Chitosan with Cerium Oxide Nanoparticles for Wound Healing Applications: Characterization and Biocompatibility Evaluation In Vitro and In Vivo.

Author

Petrova VA, Poshina DN, Golovkin AS, Mishanin AI, Zhuravskii SG, Yukina GY, Naumenko MY, Sukhorukova EG, Savin NA, Erofeev AS, Gofman IV, Ivan'kova EM, Dubashynskaya NV, Yakimansky AV, and Skorik YA

Abstract

Cerium oxide nanoparticles (CeONPs), as part of tissue regeneration matrices, can protect cells from reactive oxygen species and oxidative stress. In addition, they can influence the properties of the scaffold, including its electrospinnability and mechanical strength. In this work, we prepared electrospun fiber mats from a chitosan and polyethylene oxide blend (CS-PEO) with the addition of ceria nanoparticles (CS-PEO-CeONP). The addition of CeONPs resulted in a smaller fiber diameter and higher swelling compared to CS-PEO fiber mats. CeONP-modified fiber mats also had a higher Young's modulus due to the reinforcing effect of the nanoparticles. Both mats had comparable adhesion and cytocompatibility to mesenchymal stem cells, which had a more rounded morphology on CS-PEO-CeONP compared to elongated cells on the CS-PEO mats. Biocompatibility in an in vivo rat model showed no acute toxicity, no septic or allergic inflammation, and no rough scar tissue formation. The degradation of both mats passed the stage of matrix swelling. CS-PEO-CeONP showed significantly slower biodegradation, with most of the matrix remaining in the tissue after 90 days. The reactive inflammation was aseptic in nature with the involvement of multinucleated foreign-body type giant cells and was significantly reduced by day 90. CeONPs induced the formation of the implant's connective tissue capsule. Thus, the introduction of CeONPs influenced the physicochemical properties and biological activity of CS-PEO nanofiber mats.

Published
2024
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25. In vitro toxicity of Fe m O n , Fe m O n -SiO 2 composite, and SiO 2 -Fe m O n core-shell magnetic nanoparticles.

Author

Toropova YG, Golovkin AS, Malashicheva AB, Korolev DV, Gorshkov AN, Gareev KG, Afonin MV, and Galagudza MM

Subjects
Apoptosis drug effects, Cell Death drug effects, Cell Shape drug effects, Cell Survival drug effects, Human Umbilical Vein Endothelial Cells drug effects, Humans, Magnetite Nanoparticles ultrastructure, Magnetite Nanoparticles toxicity, Nanocomposites toxicity, Silicon Dioxide toxicity
Abstract

Over the last decade, magnetic iron oxide nanoparticles (IONPs) have drawn much attention for their potential biomedical applications. However, serious in vitro and in vivo safety concerns continue to exist. In this study, the effects of uncoated, Fe m O n -SiO 2 composite flake-like, and SiO 2 -Fe m O n core-shell IONPs on cell viability, function, and morphology were tested 48 h postincubation in human umbilical vein endothelial cell culture. Cell viability and apoptosis/necrosis rate were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and annexin V-phycoerythrin kit, respectively. Cell morphology was evaluated using bright-field microscopy and forward and lateral light scattering profiles obtained with flow cytometry analysis. All tested IONP types were used at three different doses, that is, 0.7, 7.0, and 70.0 μg. Dose-dependent changes in cell morphology, viability, and apoptosis rate were shown. At higher doses, all types of IONPs caused formation of binucleated cells suggesting impaired cytokinesis. Fe m O n -SiO 2 composite flake-like and SiO 2 -Fe m O n core-shell IONPs were characterized by similar profile of cytotoxicity, whereas bare IONPs were shown to be less toxic. The presence of either silica core or silica nanoflakes in composite IONPs can promote cytotoxic effects., Competing Interests: The authors report no conflicts of interest in this work.

Published
2017
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26. Markers of calcium and phosphate metabolism and osteopenic syndrome in patients with coronary artery disease.

Author

Golovkin AS, Kokov AN, Masenko V, Khryachkova ON, Malyuta Е, and Barbarash OL

Subjects
Aged, Biomarkers blood, Bone Density, Bone Diseases, Metabolic complications, Humans, Male, Middle Aged, Osteocalcin blood, Osteocalcin physiology, Osteopontin blood, Osteoprotegerin blood, Parathyroid Hormone blood, Vascular Calcification etiology, Bone Diseases, Metabolic metabolism, Calcium metabolism, Coronary Artery Disease metabolism, Phosphates metabolism
Abstract

Background: The aim of the present study is to assess the level of specific markers of calcium and phosphate metabolism in the development of coronary atherosclerosis in patients with stable coronary artery disease, depending on the severity of osteopenic syndrome. METHODSː In the study 112 male patients aged from 49 to 73 years with verified coronary artery disease were included in the study. Calcium Score was measured using the Agatston Score. Besides, all of them were tested on the serum level of parathyroid hormone, calcitonin, osteocalcin, bone-specific alkaline phosphatase, osteoprotegerin, osteopontin, cathepsin K, estradiol and testosterone. RESULTSː The distribution of patients according to the severity of coronary atherosclerosis using the Syntax Score suggested that the levels of the studied markers did not differ significantly among the patients, despite significant differences in the severity of coronary artery disease. The levels of osteoprotegerin in patients with mild, moderate and severe calcification were significantly lower compared to patients with a zero calcium score. There were no clinical manifestations of osteopenic syndrome in all patients. However, they underwent osteodensitometry with measurement of bone mineral density at the lumbar spine and femoral neck to determine pre-clinical symptoms of bone destruction. Significant increase (P=0.03) in serum levels of osteocalcin was found in patients with radiological evidence of osteoporosis while the other markers did no differ significantly. CONCLUSIONSː We suppose that there is a reciprocal interaction of regulatory vectors with increased calcium deposition in the arterial wall and resorption of bone tissue.

Published
2016

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