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Aims: Many empirical studies have found neutral or positive effects of forestry on plant alpha diversity in temperate deciduous forests, reflecting a positive effect of soil and canopy disturbances caused by forestry operations. The level to which this positive response to artificial disturbances mimics processes in natural forest ecosystems is less clear; hence, the systemic effects of forest management on ground floral communities remain obscure. Location: Temperate Europe, Denmark. Methods: We studied communities of ground-dwelling bryophytes and vascular plants in 400 plots distributed across 40 beech stands across four classes of forest management intensity – long unmanaged, recently unmanaged, near-to-nature managed, and shelterwood managed – while recording the incidence of a diverse array of microhabitats related to hydrology, canopy closure and soil disturbance. Results: Microhabitat availability differed considerably among forest management classes as a response to management. Overall, forest management had a significant positive effect on the plot-level alpha diversity of both vascular plants and bryophytes. In contrast, beta diversity and total ecospace decreased with forest management intensity. Litter accumulation simultaneously decreased alpha diversity in both groups, while light availability, soil exposure and presence of wetlands respectively, were crucial for vascular plant and bryophyte richness. Forestry-created canopy gaps had a similar effect on alpha diversity as natural tree-fall gaps but supported a different set of species, mainly ubiquitous generalists. Conclusions: Even if plant alpha diversity was lower in unmanaged forest stands, this did not significantly affect gamma diversity across the sampled stands, suggesting that plant richness needs to be considered at a relevant scale when evaluating forest management impact on biodiversity. These scaling issues seem to reflect fundamental differences in disturbance dynamics in managed and un

PURPOSE: To report the relative frequencies of childhood and early onset glaucoma subtypes and their genetic findings in a large single cohort. DESIGN: Retrospective clinical and molecular study. PARTICIPANTS: All individuals with childhood glaucoma (diagnosed 0 to <18 years) and early onset glaucoma (diagnosed 18 to <40 years) referred to a national disease registry. METHODS: We retrospectively reviewed the referrals of all individuals with glaucoma diagnosed at <40 years of age recruited to the Australian and New Zealand Registry of Advanced Glaucoma (ANZRAG). Subtypes of glaucoma were determined using the Childhood Glaucoma Research Network (CGRN) classification system. DNA extracted from blood or saliva samples underwent sequencing of genes associated with glaucoma. MAIN OUTCOME MEASURES: The phenotype and genotype distribution of glaucoma diagnosed at <40 years of age. RESULTS: A total of 290 individuals (533 eyes) with childhood glaucoma and 370 individuals (686 eyes) with early onset glaucoma were referred to the ANZRAG. Primary glaucoma was the most prevalent condition in both cohorts. In the childhood cohort, 57.6% of individuals (167/290, 303 eyes) had primary congenital glaucoma (PCG), and 19.3% (56/290, 109 eyes) had juvenile open-angle glaucoma. Juvenile open-angle glaucoma constituted 73.2% of the early onset glaucoma cohort (271/370, 513 eyes). Genetic testing in probands resulted in a diagnostic yield of 24.7% (125/506) and a reclassification of glaucoma subtype in 10.4% of probands (13/125). The highest molecular diagnostic rate was achieved in probands with glaucoma associated with nonacquired ocular anomalies (56.5%). Biallelic variants in CYP1B1 (n = 29, 23.2%) and heterozygous variants in MYOC (n = 24, 19.2%) and FOXC1 (n = 21, 16.8%) were most commonly reported among probands with a molecular diagnosis. Biallelic CYP1B1 variants were reported in twice as many female individuals as male individuals with PCG (66.7% vs. 33.3%, P = 0.02). CONCLUSIO

OBJECTIVE: To describe the development and implementation of a web-based high-quality data collection tool to track the outcomes of glaucoma treatments in routine practice. METHODS AND ANALYSIS: This is a prospective observational registry study. An international steering committee undertook an iterative structured process to define a minimum, patient-centred data set designed to track outcomes of glaucoma treatment. The outcomes were coded into a web-based programme allowing easy access for rapid data entry. Clinicians receive personal reports enabling instant audit of their outcomes. Analyses of aggregated anonymised data on real-world outcomes are analysed and periodically reported with the goal of improving patient care. RESULTS: The minimum data set developed by the international steering committee includes the following: a baseline visit captures 13 mandatory fields in order to accurately phenotype each patient's subtype of glaucoma and to allow comparison between services, and a follow-up visit includes only four mandatory fields to allow completion within 30 s.Currently, there are 157 surgeons in 158 ophthalmology practices across Australia and New Zealand who are registered. These surgeons are tracking 5570 eyes of 3001 patients and have recorded 67 074 visits. The median number of eyes per surgeon is 22 eyes with a range of 1-575. The most common glaucoma procedure, excluding cataract surgery, is iStent inject, with 2316 cases. CONCLUSION: This software tool effectively facilitates data collection on safety and efficacy outcomes of treatments for different subgroups of glaucoma within a real-world setting. It provides a template to evaluate new treatments as they are introduced into practice.