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4. Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer

Author

Bao, Macus Hao-Ran, Yang, Chunxue, Tse, Aki Pui-Wah, Wei, Lai, Lee, Derek, Zhang, Misty Shuo, Goh, Chi Ching, Chiu, David Kung-Chun, Yuen, Vincent Wai-Hin, Law, Cheuk-Ting, Chin, Wai-Ching, Chui, Noreen Nog-Qin, Wong, Bowie Po-Yee, Chan, Cerise Yuen-Ki, Ng, Irene Oi-Lin, Chung, Clive Yik-Sham, Wong, Chun-Ming, and Wong, Carmen Chak-Lui

Published
2021
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5. A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils

Author

Janela, Baptiste, Patel, Amit A., Lau, Mai Chan, Goh, Chi Ching, Msallam, Rasha, Kong, Wan Ting, Fehlings, Michael, Hubert, Sandra, Lum, Josephine, Simoni, Yannick, Malleret, Benoit, Zolezzi, Francesca, Chen, Jinmiao, Poidinger, Michael, Satpathy, Ansuman T., Briseno, Carlos, Wohn, Christian, Malissen, Bernard, Murphy, Kenneth M., Maini, Alexander A., Vanhoutte, Leen, Guilliams, Martin, Vial, Emmanuel, Hennequin, Laurent, Newell, Evan, Ng, Lai Guan, Musette, Philippe, Yona, Simon, Hacini-Rachinel, Feriel, and Ginhoux, Florent

Published
2019
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6. Hyaluronan Receptor LYVE-1-Expressing Macrophages Maintain Arterial Tone through Hyaluronan-Mediated Regulation of Smooth Muscle Cell Collagen

Author

Lim, Hwee Ying, Lim, Sheau Yng, Tan, Chek Kun, Thiam, Chung Hwee, Goh, Chi Ching, Carbajo, Daniel, Chew, Samantha Hui Shang, See, Peter, Chakarov, Svetoslav, Wang, Xiao Nong, Lim, Li Hui, Johnson, Louise A., Lum, Josephine, Fong, Chui Yee, Bongso, Ariff, Biswas, Arijit, Goh, Chern, Evrard, Maximilien, Yeo, Kim Pin, Basu, Ranu, Wang, Jun Kit, Tan, Yingrou, Jain, Rohit, Tikoo, Shweta, Choong, Cleo, Weninger, Wolfgang, Poidinger, Michael, Stanley, Richard E., Collin, Matthew, Tan, Nguan Soon, Ng, Lai Guan, Jackson, David G., Ginhoux, Florent, and Angeli, Véronique

Published
2018
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8. Dengue virus-elicited tryptase induces endothelial permeability and shock

Author

Rathore, Abhay P.S., Mantri, Chinmay Kumar, Aman, Siti A.B., Syenina, Ayesa, Ooi, Justin, Jagaraj, Cyril J., Goh, Chi Ching, Tissera, Hasitha, Wilder-Smith, Annelies, Ng, Lai Guan, Gubler, Duane J., and John, Ashley L. St.

Subjects
Angiotensin II -- Health aspects, Hemorrhagic fevers -- Health aspects, Endothelium -- Health aspects, Proteases -- Health aspects, Permeability -- Health aspects, Infection -- Health aspects, Dengue virus -- Health aspects, Shock -- Health aspects, Health care industry
Abstract

Dengue virus (DENV) infection causes a characteristic pathology in humans involving dysregulation of the vascular system. In some patients with dengue hemorrhagic fever (DHF), vascular pathology can become severe, resulting in extensive microvascular permeability and plasma leakage into tissues and organs. Mast cells (MCs), which line blood vessels and regulate vascular function, are able to detect DENV in vivo and promote vascular leakage. Here, we showed that an MC-derived protease, tryptase, is consequential for promoting vascular permeability during DENV infection through inducing breakdown of endothelial cell tight junctions. Injected tryptase alone was sufficient to induce plasma loss from the circulation and hypovolemic shock in animals. A potent tryptase inhibitor, nafamostat mesylate, blocked DENV-induced vascular leakage in vivo. Importantly, in 2 independent human dengue cohorts, tryptase levels correlated with the grade of DHF severity. This study defines an immune mechanism by which DENV can induce vascular pathology and shock., Introduction In humans, dengue virus (DENV) causes an acute viral infection that manifests as a broad spectrum of disease: from asymptomatic infection to a mild febrile illness, dengue fever (DF), [...]

Published
2019
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10. Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function

Author

Takata, Kazuyuki, Kozaki, Tatsuya, Lee, Christopher Zhe Wei, Thion, Morgane Sonia, Otsuka, Masayuki, Lim, Shawn, Utami, Kagistia Hana, Fidan, Kerem, Park, Dong Shin, Malleret, Benoit, Chakarov, Svetoslav, See, Peter, Low, Donovan, Low, Gillian, Garcia-Miralles, Marta, Zeng, Ruizhu, Zhang, Jinqiu, Goh, Chi Ching, Gul, Ahmet, Hubert, Sandra, Lee, Bernett, Chen, Jinmiao, Low, Ivy, Shadan, Nurhidaya Binte, Lum, Josephine, Wei, Tay Seok, Mok, Esther, Kawanishi, Shohei, Kitamura, Yoshihisa, Larbi, Anis, Poidinger, Michael, Renia, Laurent, Ng, Lai Guan, Wolf, Yochai, Jung, Steffen, Önder, Tamer, Newell, Evan, Huber, Tara, Ashihara, Eishi, Garel, Sonia, Pouladi, Mahmoud A., and Ginhoux, Florent

Published
2017
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11. Hypoxia-inducible factor orchestrates adenosine metabolism to promote liver cancer development

Author

Cheu, Jacinth Wing-Sum, primary, Chiu, David Kung-Chun, additional, Kwan, Kenneth Kin-Leung, additional, Yang, Chunxue, additional, Yuen, Vincent Wai-Hin, additional, Goh, Chi Ching, additional, Chui, Noreen Nog-Qin, additional, Shen, Wei, additional, Law, Cheuk-Ting, additional, Li, Qidong, additional, Zhang, Misty Shuo, additional, Bao, Macus Hao-Ran, additional, Wong, Bowie Po-Yee, additional, Chan, Cerise Yuen-Ki, additional, Liu, Cindy Xinqi, additional, Sit, Grace Fu-Wan, additional, Ooi, Zher Yee, additional, Deng, Haijing, additional, Tse, Aki Pui-Wah, additional, Ng, Irene Oi-Lin, additional, and Wong, Carmen Chak-Lui, additional

Published
2023
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12. Neutrophils Self-Regulate Immune Complex-Mediated Cutaneous Inflammation through CXCL2

Author

Li, Jackson LiangYao, Lim, Chun Hwee, Tay, Fen Wei, Goh, Chi Ching, Devi, Sapna, Malleret, Benoit, Lee, Bernett, Bakocevic, Nadja, Chong, Shu Zhen, Evrard, Maximilien, Tanizaki, Hideaki, Lim, Hwee Ying, Russell, Bruce, Renia, Laurent, Zolezzi, Francesca, Poidinger, Michael, Angeli, Veronique, St. John, Ashley L., Harris, John E., Tey, Hong Liang, Tan, Suet Mien, Kabashima, Kenji, Weninger, Wolfgang, Larbi, Anis, and Ng, Lai Guan

Published
2016
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13. Polo‐like kinase 4 inhibitor CFI‐400945 suppresses liver cancer through cell cycle perturbation and eliciting antitumor immunity

Author

Chan, Cerise Yuen‐Ki, primary, Yuen, Vincent Wai‐Hin, additional, Chiu, David Kung‐Chun, additional, Goh, Chi‐Ching, additional, Thu, Kelsie L., additional, Cescon, David W., additional, Soria‐Bretones, Isabel, additional, Law, Cheuk‐Ting, additional, Cheu, Jacinth Wing‐Sum, additional, Lee, Derek, additional, Tse, Aki Pui‐Wah, additional, Tan, Kel Vin, additional, Zhang, Misty Shuo, additional, Wong, Bowie Po‐Yee, additional, Wong, Chun‐Ming, additional, Khong, Pek‐Lan, additional, Ng, Irene Oi‐Lin, additional, Bray, Mark R., additional, Mak, Tak Wah, additional, Yau, Thomas Chung‐Cheung, additional, and Wong, Carmen Chak‐Lui, additional

Published
2023
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17. Transitional premonocytes emerge in the periphery for host defense against bacterial infections

Author

Teh, Ye Chean, primary, Chooi, Ming Yao, additional, Liu, Dehua, additional, Kwok, Immanuel, additional, Lai, Ghee Chuan, additional, Ayub Ow Yong, Liyana, additional, Ng, Melissa, additional, Li, Jackson L. Y., additional, Tan, Yingrou, additional, Evrard, Maximilien, additional, Tan, Leonard, additional, Liong, Ka Hang, additional, Leong, Keith, additional, Goh, Chi Ching, additional, Chan, Andrew Y. J., additional, Shadan, Nurhidaya Binte, additional, Mantri, Chinmay Kumar, additional, Hwang, You Yi, additional, Cheng, Hui, additional, Cheng, Tao, additional, Yu, Weimiao, additional, Tey, Hong Liang, additional, Larbi, Anis, additional, St John, Ashley, additional, Angeli, Veronique, additional, Ruedl, Christiane, additional, Lee, Bernett, additional, Ginhoux, Florent, additional, Chen, Swaine L., additional, Ng, Lai Guan, additional, Ding, Jeak Ling, additional, and Chong, Shu Zhen, additional

Published
2022
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19. Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function

Author

Takata, Kazuyuki, primary, Kozaki, Tatsuya, additional, Lee, Christopher Zhe Wei, additional, Thion, Morgane Sonia, additional, Otsuka, Masayuki, additional, Lim, Shawn, additional, Utami, Kagistia Hana, additional, Fidan, Kerem, additional, Park, Dong Shin, additional, Malleret, Benoit, additional, Chakarov, Svetoslav, additional, See, Peter, additional, Low, Donovan, additional, Low, Gillian, additional, Garcia-Miralles, Marta, additional, Zeng, Ruizhu, additional, Zhang, Jinqiu, additional, Goh, Chi Ching, additional, Gul, Ahmet, additional, Hubert, Sandra, additional, Lee, Bernett, additional, Chen, Jinmiao, additional, Low, Ivy, additional, Shadan, Nurhidaya Binte, additional, Lum, Josephine, additional, Wei, Tay Seok, additional, Mok, Esther, additional, Kawanishi, Shohei, additional, Kitamura, Yoshihisa, additional, Larbi, Anis, additional, Poidinger, Michael, additional, Renia, Laurent, additional, Ng, Lai Guan, additional, Wolf, Yochai, additional, Jung, Steffen, additional, Önder, Tamer, additional, Newell, Evan, additional, Huber, Tara, additional, Ashihara, Eishi, additional, Garel, Sonia, additional, Pouladi, Mahmoud A., additional, and Ginhoux, Florent, additional

Published
2020
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20. Inhibition of CMTM4 Sensitizes Cholangiocarcinoma and Hepatocellular Carcinoma to T Cell–Mediated Antitumor Immunity Through PD‐L1.

Author

Chui, Noreen Nog‐Qin, Cheu, Jacinth Wing‐Sum, Yuen, Vincent Wai‐Hin, Chiu, David Kung‐Chun, Goh, Chi‐Ching, Lee, Derek, Zhang, Misty Shuo, Ng, Irene Oi‐Lin, and Wong, Carmen Chak‐Lui

Subjects
PROGRAMMED death-ligand 1, CELLULAR immunity, HEPATOCELLULAR carcinoma, IMMUNE checkpoint inhibitors, TUMOR markers, T cell receptors, CHOLANGIOCARCINOMA, CELL-mediated cytotoxicity
Abstract

Liver cancers consist primarily of hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC). Immune checkpoint inhibitors have emerged as promising therapeutic agents against liver cancers. Programmed cell death protein 1 (PD‐1) is an immunoinhibitory receptor present on T cells that interacts with its ligand programmed death‐ligand 1 (PD‐L1) found on cancer cells. Blocking PD‐1/PD‐L1 binding improves T‐cell survival, proliferation and cytotoxicity, which enhances their antitumor activity. Better understanding of the molecular mechanisms governing PD‐1/PD‐L1 response is essential to the development of predictive markers and therapeutic combinations that could improve the efficiency of anti‐PD‐1/PD‐L1 treatment. Chemokine‐like factor (CKLF)–like MARVEL transmembrane domain–containing 6 (CMTM6) has been recently identified as a major regulator of PD‐L1. Another member in the CMTM family, CKLF‐like MARVEL transmembrane domain–containing 4 (CMTM4), has been shown to compensate for the effects of CMTM6 when CMTM6 is lost. Interestingly, we found that CMTM4 is the major regulator of PD‐L1 in the context of liver cancer. Up‐regulated CMTM4 in patients with HCC and ICC is associated with poor patient survival, potentially due to its function in stabilizing PD‐L1 expression, hence facilitating escape from T cell–mediated cytotoxicity. We confirmed the role of CMTM4 as a positive regulator of PD‐L1 in multiple HCC and ICC cell lines and demonstrated that CMTM4 stabilizes PD‐L1 through posttranslational mechanisms. In vivo, suppression of Cmtm4 inhibited HCC growth and increased CD8+ T‐cell infiltration in immunocompetent mice. Furthermore, we found that depletion of CMTM4 sensitized HCC tumor to anti‐PD‐L1 treatment compared with control. This suggests that CMTM4 expression level could be a predictive marker for patient response to anti‐PD‐L1 treatment, and CMTM4 depletion can potentially be used to enhance the clinical benefits of anti‐PD‐L1 immunotherapy in patients with liver cancer. [ABSTRACT FROM AUTHOR]

Published
2022
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24. Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions

Author

José M. Adrover, Subhra K. Biswas, Shu Zhen Chong, Jackson LiangYao Li, Anis Larbi, Zhiyong Poon, Etienne Becht, Shihui Foo, Goh Chi Ching, Ka Hang Liong, Hweixian Leong Penny, Florent Ginhoux, Maximilien Evrard, Je Lin Sieow, Karl Balabanian, Jia Wang Chua, Françoise Bachelerie, Vikas Madan, Andrés Hidalgo, I-hsin Su, William Hwang, Lai Guan Ng, Immanuel Kwok, Siew Cheng Wong, Karen Wei Weng Teng, Sapna Devi, Leonard Tan, Jinmiao Chen, H. Phillip Koeffler, Evan W. Newell, and School of Biological Sciences

Subjects
0301 basic medicine, Granulopoiesis, Neutrophils, Immunology, Inflammation, Bone Marrow Cells, Biology, Transcriptome, 03 medical and health sciences, Mice, 0302 clinical medicine, trafficking, Cell Movement, medicine, Immunology and Allergy, Animals, Humans, Mass cytometry, Cell Lineage, Cells, Cultured, Progenitor, Cell Proliferation, Ccaat-enhancer-binding proteins, Effector, Gene Expression Profiling, Biological sciences [Science], Neoplasms, Experimental, neutrophil ontogeny, Cell biology, neutrophil precursors, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, 030220 oncology & carcinogenesis, CCAAT-Enhancer-Binding Proteins, Bone marrow, medicine.symptom, neutrophil development
Abstract

Neutrophils are specialized innate cells that require constant replenishment from proliferative bone marrow (BM) precursors as a result of their short half-life. Although it is established that neutrophils are derived from the granulocyte-macrophage progenitor (GMP), the differentiation pathways from GMP to functional mature neutrophils are poorly defined. Using mass cytometry (CyTOF) and cell-cycle-based analysis, we identified three neutrophil subsets within the BM: a committed proliferative neutrophil precursor (preNeu) which differentiates into non-proliferating immature neutrophils and mature neutrophils. Transcriptomic profiling and functional analysis revealed that preNeu require the C/EBPε transcription factor for their generation from the GMP, and their proliferative program is substituted by a gain of migratory and effector function as they mature. preNeus expand under microbial and tumoral stress, and immature neutrophils are recruited to the periphery of tumor-bearing mice. In summary, our study identifies specialized BM granulocytic populations that ensure supply under homeostasis and stress responses. We thank all members of L.G.N laboratory, the SIgN (Singapore Immunology Network) flow-cytometry team, the SIgN functional genomics team for their assistance with transcriptomics, and the SIgN mouse core facility for their technical help and support. This research was funded by SIgN core funding, A*STAR (Agency for Science, Technology and Research), Singapore. Sí

Published
2017

25. The impact of ischemia‐reperfusion injuries on skin resident murine dendritic cells

Author

Goh, Chi Ching, primary, Evrard, Maximilien, additional, Chong, Shu Zhen, additional, Tan, Yingrou, additional, Tan, Leonard De Li, additional, Teng, Karen Wei Weng, additional, Weninger, Wolfgang, additional, Becker, David Laurence, additional, Tey, Hong Liang, additional, Newell, Evan William, additional, Liu, Bin, additional, and Ng, Lai Guan, additional

Published
2018
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26. Theranostics: Efficient Red/Near-Infrared Fluorophores Based on Benzo[1,2-b :4,5-b ′]dithiophene 1,1,5,5-Tetraoxide for Targeted Photodynamic Therapy and In Vivo Two-Photon Fluorescence Bioimaging (Adv. Funct. Mater. 13/2018)

Author

Zhen, Shijie, primary, Wang, Shaowei, additional, Li, Shiwu, additional, Luo, Wenwen, additional, Gao, Meng, additional, Ng, Lai Guan, additional, Goh, Chi Ching, additional, Qin, Anjun, additional, Zhao, Zujin, additional, Liu, Bin, additional, and Tang, Ben Zhong, additional

Published
2018
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27. Efficient Red/Near-Infrared Fluorophores Based on Benzo[1,2-b:4,5-b′]dithiophene 1,1,5,5-Tetraoxide for Targeted Photodynamic Therapy and In Vivo Two-Photon Fluorescence Bioimaging

Author

Zhen, Shijie, Wang, Shaowei, Li, Shiwu, Luo, Wenwen, Gao, Meng, Ng, Lai Guan, Goh, Chi Ching, Qin, Anjun, Zhao, Zujin, Liu, Bin, Tang, Benzhong, Zhen, Shijie, Wang, Shaowei, Li, Shiwu, Luo, Wenwen, Gao, Meng, Ng, Lai Guan, Goh, Chi Ching, Qin, Anjun, Zhao, Zujin, Liu, Bin, and Tang, Benzhong

Abstract

Red/near-infrared dyes are highly demanded for biological applications but most of them are far from satisfactory. In this work, a series of red/near-infrared fluorophores based on electron-withdrawing benzo[1,2-b:4,5-b′]dithiophene 1,1,5,5-tetraoxide (BDTO) are synthesized and characterized. They possess both aggregation-induced emission, and hybridized local and charge-transfer characteristics. Crystallographic, spectroscopic, electrochemical and computational results reveal that the oxidation of benzo[1,2-b:4,5-b′]dithiophene to BDTO can endow the fluorophores with greatly red-shifted emission, enhanced emission efficiency, reduced energy levels, enlarged two-photon absorption cross section, and increased reactive oxygen species generation efficiency. The nanoparticles fabricated with a near-infrared fluorophore TPA-BDTO show high photostability and biocompatibility with good performance in targeted photodynamic ablation of cancer cells and two-photon fluorescence imaging of intravital mouse brain vasculature. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Published
2018

28. Polymeric Nanorods with Aggregation-induced Emission Characteristics for Enhanced Cancer Targeting and Imaging

Author

Feng, Guangxue, Mao, Duo, Liu, Jie, Goh, Chi Ching, Ng, Lai Guan, Kong, Deling, Tang, Benzhong, Liu, Bin, Feng, Guangxue, Mao, Duo, Liu, Jie, Goh, Chi Ching, Ng, Lai Guan, Kong, Deling, Tang, Benzhong, and Liu, Bin

Abstract

Polymeric nanorods loaded with AIEgens are synthesized via nano-precipitation under ultrasound sonication, where prolonged sonication time could induce a nanodot-to-nanorod transition. These AIE nanorods, but not the nanodots, could be selectively internalized into cancer cells, which show better tumor accumulation, higher tumor penetration and more efficient in vivo cancer cell uptake.

Published
2018

30. Efficient Red/Near-Infrared Fluorophores Based on Benzo[1,2-b :4,5-b ′]dithiophene 1,1,5,5-Tetraoxide for Targeted Photodynamic Therapy and In Vivo Two-Photon Fluorescence Bioimaging

Author

Zhen, Shijie, primary, Wang, Shaowei, additional, Li, Shiwu, additional, Luo, Wenwen, additional, Gao, Meng, additional, Ng, Lai Guan, additional, Goh, Chi Ching, additional, Qin, Anjun, additional, Zhao, Zujin, additional, Liu, Bin, additional, and Tang, Ben Zhong, additional

Published
2018
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35. Efficient Red/Near‐Infrared Fluorophores Based on Benzo[1,2‐<italic>b</italic>:4,5‐<italic>b</italic>′]dithiophene 1,1,5,5‐Tetraoxide for Targeted Photodynamic Therapy and In Vivo Two‐Photon Fluorescence Bioimaging.

Author

Zhen, Shijie, Wang, Shaowei, Li, Shiwu, Luo, Wenwen, Gao, Meng, Ng, Lai Guan, Goh, Chi Ching, Qin, Anjun, Zhao, Zujin, Liu, Bin, and Tang, Ben Zhong

Subjects
FLUOROPHORES, PHOTODYNAMIC therapy, PHOTONS, FLUORESCENCE, BIO-imaging sensors, BLOOD vessels
Abstract

Abstract: Red/near‐infrared dyes are highly demanded for biological applications but most of them are far from satisfactory. In this work, a series of red/near‐infrared fluorophores based on electron‐withdrawing benzo[1,2‐b:4,5‐b′]dithiophene 1,1,5,5‐tetraoxide (BDTO) are synthesized and characterized. They possess both aggregation‐induced emission, and hybridized local and charge‐transfer characteristics. Crystallographic, spectroscopic, electrochemical and computational results reveal that the oxidation of benzo[1,2‐b:4,5‐b′]dithiophene to BDTO can endow the fluorophores with greatly red‐shifted emission, enhanced emission efficiency, reduced energy levels, enlarged two‐photon absorption cross section, and increased reactive oxygen species generation efficiency. The nanoparticles fabricated with a near‐infrared fluorophore TPA‐BDTO show high photostability and biocompatibility with good performance in targeted photodynamic ablation of cancer cells and two‐photon fluorescence imaging of intravital mouse brain vasculature. [ABSTRACT FROM AUTHOR]

Published
2018
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37. Biocompatible green and red fluorescent organic dots with remarkably large two-photon action cross sections for targeted cellular imaging and real-time intravital blood vascular visualization

Author

Xiang, Jiayun, Cai, Xiaolei, Lou, Xiaoding, Feng, Guangxue, Min, Xuehong, Luo, Wenwen, He, Bairong, Goh, Chi Ching, Ng, Lai Guan, Zhou, Jian, Zhao, Zujin, Liu, Bin, Tang, Ben Zhong, Xiang, Jiayun, Cai, Xiaolei, Lou, Xiaoding, Feng, Guangxue, Min, Xuehong, Luo, Wenwen, He, Bairong, Goh, Chi Ching, Ng, Lai Guan, Zhou, Jian, Zhao, Zujin, Liu, Bin, and Tang, Ben Zhong

Abstract

Fluorescent organic dots are emerging as promising bioimaging reagents because of their high brightness, good photostability, excellent biocompatibility, and facile surface functionalization. Organic dots with large two-photon absorption (TPA) cross sections are highly desired for two-photon fluorescence microscopy. In this work, we report two biocompatible and photostable organic dots fabricated by encapsulating tetraphenylethene derivatives within DSPE-PEG matrix. The two organic dots show absorption maxima at 425 and 483 nm and emit green and red fluorescence at 560 and 645 nm, with high fluorescence quantum yields of 64% and 22%, respectively. Both organic dots exhibit excellent TPA property in the range of 800-960 nm, affording upon excitation at 820 nm remarkably large TPA cross sections of 1.2 × 106 and 2.5 × 106 GM on the basis of dot concentration. The bare fluorophores and their organic dots are biocompatible and have been used to stain living cells for one- and two-photon fluorescence bioimagings. The cRGD-modified organic dots can selectively target integrin αvβ3 overexpressing breast cancer cells for targeted imaging. The organic dots are also applied for real-time two-photon fluorescence in vivo visualization of the blood vasculature of mouse ear, providing the spatiotemporal information about the whole blood vascular network. These results demonstrate that the present fluorescent organic dots are promising candidates for living cell and tissue imaging. © 2015 American Chemical Society.

Published
2015

38. Biocompatible Green and Red Fluorescent Organic Dots with Remarkably Large Two-Photon Action Cross Sections for Targeted Cellular Imaging and Real-Time Intravital Blood Vascular Visualization

Author

Xiang, Jiayun, primary, Cai, Xiaolei, additional, Lou, Xiaoding, additional, Feng, Guangxue, additional, Min, Xuehong, additional, Luo, Wenwen, additional, He, Bairong, additional, Goh, Chi Ching, additional, Ng, Lai Guan, additional, Zhou, Jian, additional, Zhao, Zujin, additional, Liu, Bin, additional, and Tang, Ben Zhong, additional

Published
2015
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40. Red Emissive Biocompatible Nanoparticles from Tetraphenylethene-Decorated BODIPY Luminogens for Two-Photon Excited Fluorescence Cellular Imaging and Mouse Brain Blood Vascular Visualization

Author

Zhao, Zujin, Chen, Bin, Geng, Junlong, Chang, Zhengfeng, Aparicio-Ixta, Laura, Nie, Han, Goh, Chi Ching, Ng, Lai Guan, Qin, Anjun, Ramos-Ortiz, Gabriel, Liu, Bin, Tang, Ben Zhong, Zhao, Zujin, Chen, Bin, Geng, Junlong, Chang, Zhengfeng, Aparicio-Ixta, Laura, Nie, Han, Goh, Chi Ching, Ng, Lai Guan, Qin, Anjun, Ramos-Ortiz, Gabriel, Liu, Bin, and Tang, Ben Zhong

Abstract

BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) is an emissive chromophore in solutions but suffers from fluorescence quenching when aggregated due to its flat molecular conformation and small Stokes shift. To create aggregate-state emissive BODIPY luminogens, tetraphenylethene (TPE), which is a popular luminogen with intriguing aggregation-induced emission (AIE) characteristic, is introduced as periphery to a methylated BODIPY core. Three TPE-BODIPY adducts are synthesized and characterized, and their photophysical properties and electronic structures are investigated. The incorporation of AIE-active TPE units alleviates aggregation-caused quenching of BODIPY core, furnishing emissive nanoparticles based on TPE-BODIPY adducts. Significantly, the two-photon absorption (TPA) and two-photon excited fluorescence (TPEF) properties are improved as more TPE units are attached. The luminogens with 3TPE units (3TPE-BODIPY) shows the strongest TPA and TPEF in the wavelength range of 750-830 nm, with cross-section values of 264 and 116 GM at 810 nm, respectively. Red emissive nanoparticles with a Stokes shift of 60 nm and a fluorescence quantum yield of 16% are attained by encapsulating 3TPE-BODIPY with 1,2-sistearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylen e glycol)-2000]. The nanoparticles are biocompatible and function well in TPEF cellular imaging and mouse brain blood vascular visualization.

Published
2014

41. CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections

Author

Bakocevic, Nadja, primary, Claser, Carla, additional, Yoshikawa, Soichiro, additional, Jones, Leigh Ann, additional, Chew, Samantha, additional, Goh, Chi Ching, additional, Malleret, Benoit, additional, Larbi, Anis, additional, Ginhoux, Florent, additional, de Lafaille, Maria Curotto, additional, Karasuyama, Hajime, additional, Renia, Laurent, additional, and Ng, Lai Guan, additional

Published
2014
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42. Imaging: Red Emissive Biocompatible Nanoparticles from Tetraphenylethene-Decorated BODIPY Luminogens for Two-Photon Excited Fluorescence Cellular Imaging and Mouse Brain Blood Vascular Visualization (Part. Part. Syst. Charact. 4/2014)

Author

Zhao, Zujin, primary, Chen, Bin, additional, Geng, Junlong, additional, Chang, Zhengfeng, additional, Aparicio-Ixta, Laura, additional, Nie, Han, additional, Goh, Chi Ching, additional, Ng, Lai Guan, additional, Qin, Anjun, additional, Ramos-Ortiz, Gabriel, additional, Liu, Bin, additional, and Tang, Ben Zhong, additional

Published
2014
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44. Ultrabright Organic Dots with Aggregation-Induced Emission Characteristics for Real-Time Two-Photon Intravital Vasculature Imaging

Author

Ding, Dan, Goh, Chi Ching, Feng, Guangxue, Zhao, Zujin, Liu, Jie, Liu, Rongrong, Tomczak, Nikodem, Geng, Junlong, Tang, Ben Zhong, Ng, Lai Guan, Liu, Bin, Ding, Dan, Goh, Chi Ching, Feng, Guangxue, Zhao, Zujin, Liu, Jie, Liu, Rongrong, Tomczak, Nikodem, Geng, Junlong, Tang, Ben Zhong, Ng, Lai Guan, and Liu, Bin

Abstract

Ultrabright organic dots with aggregation-induced emission characteristics (AIE dots) are prepared and shown to exhibit a high quantum yield, a, large two-photon absorption cross-section, and low in vivo toxicity. Real-time two-photon intravital blood vascular imaging in various tissues substantiates that the AIE dots are effective probes for in vivo vasculature imaging in a deep and high-contrast manner. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Published
2013

45. Red Emissive Biocompatible Nanoparticles from Tetraphenylethene-Decorated BODIPY Luminogens for Two-Photon Excited Fluorescence Cellular Imaging and Mouse Brain Blood Vascular Visualization

Author

Zhao, Zujin, primary, Chen, Bin, additional, Geng, Junlong, additional, Chang, Zhengfeng, additional, Aparicio-Ixta, Laura, additional, Nie, Han, additional, Goh, Chi Ching, additional, Ng, Lai Guan, additional, Qin, Anjun, additional, Ramos-Ortiz, Gabriel, additional, Liu, Bin, additional, and Tang, Ben Zhong, additional

Published
2013
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46. Neutrophil mobilization via plerixafor-mediated CXCR4 inhibition arises from lung demargination and blockade of neutrophil homing to the bone marrow

Author

Devi, Sapna, primary, Wang, Yilin, additional, Chew, Weng Keong, additional, Lima, Ronald, additional, A-González, Noelia, additional, Mattar, Citra N.Z., additional, Chong, Shu Zhen, additional, Schlitzer, Andreas, additional, Bakocevic, Nadja, additional, Chew, Samantha, additional, Keeble, Jo L., additional, Goh, Chi Ching, additional, Li, Jackson L.Y., additional, Evrard, Maximilien, additional, Malleret, Benoit, additional, Larbi, Anis, additional, Renia, Laurent, additional, Haniffa, Muzlifah, additional, Tan, Suet Mien, additional, Chan, Jerry K.Y., additional, Balabanian, Karl, additional, Nagasawa, Takashi, additional, Bachelerie, Françoise, additional, Hidalgo, Andrés, additional, Ginhoux, Florent, additional, Kubes, Paul, additional, and Ng, Lai Guan, additional

Published
2013
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