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Objective(s): We aimed to investigate the levels of transient receptor potential melastatin (TRPM) gene expression, and the antioxidant and histopathologic effect of thymoquinone (Tmq) in the hepatic I/R rat model.Materials and Methods: Fifty Wistar rats were divided into 5 groups. Group 1: Control; Group 2: Sham; Group 3: Hepatic I/R (45 min/45 min); Group 4: Tmq (50 mg/kg); Group 5: Tmq+I/R (ten days before I/R at the dose of 50 mg/kg of Tmq). The hepatic I/R (45min/45min) model was performed at the portal vein and the hepatic artery with atraumatic vascular clamp in the ischemia groups. The liver tissues and blood samples that were taken at the end of the study were evaluated for histopathologic and biochemical analysis. Besides TRPM gene expression levels were determined in liver tissues. It was seen that cellular swelling, congestion, PNL, and apoptosis parameters statistically decreased in Tmq and Tmq+I/R groups in comparison with the I/R group in histopathological evaluation.Results: It was observed that biochemical parameters, AST, ALT, GGT, LDH, creatinine, and urea levels significantly increased in the I/R group as compared with, sham, Tmq, and Tmq+I/R groups. It was found that TRPM2,6,7,8 gene expression decreased significantly in Tmq+I/R groups as compared to the I/R group. Conclusion: We showed that thymoquinone can inhibit the entry of Ca+2 into the cell by decreasing TRPM2,6,7,8 gene expression. Based on our findings, we think that Tmq application in the treatment of liver diseases due to I/R damage may be important in terms of both ischemia and apoptosis and can also be used in the treatment of liver-related diseases.

Objective(s): We aimed to investigate the levels of transient receptor potential melastatin (TRPM) gene expression, and the antioxidant and histopathologic effect of thymoquinone (Tmq) in the hepatic I/R rat model. Materials and Methods: Fifty Wistar rats were divided into 5 groups. Group 1: Control; Group 2: Sham; Group 3: Hepatic I/R (45 min/45 min); Group 4: Tmq (50 mg/kg); Group 5: Tmq+I/R (ten days before I/R at the dose of 50 mg/kg of Tmq). The hepatic I/R (45min/45min) model was performed at the portal vein and the hepatic artery with atraumatic vascular clamp in the ischemia groups. The liver tissues and blood samples that were taken at the end of the study were evaluated for histopathologic and biochemical analysis. Besides TRPM gene expression levels were determined in liver tissues. It was seen that cellular swelling, congestion, PNL, and apoptosis parameters statistically decreased in Tmq and Tmq+I/R groups in comparison with the I/R group in histopathological evaluation. Results: It was observed that biochemical parameters, AST, ALT, GGT, LDH, creatinine, and urea levels significantly increased in the I/R group as compared with, sham, Tmq, and Tmq+I/R groups. It was found that TRPM2,6,7,8 gene expression decreased significantly in Tmq+I/R groups as compared to the I/R group. Conclusion: We showed that thymoquinone can inhibit the entry of Ca+2 into the cell by decreasing TRPM2,6,7,8 gene expression. Based on our findings, we think that Tmq application in the treatment of liver diseases due to I/R damage may be important in terms of both ischemia and apoptosis and can also be used in the treatment of liver-related diseases. [ABSTRACT FROM AUTHOR]

In this work, [Mg.sub.65][Ni.sub.20][Y.sub.15-X][Ag.sub.X] (X = 1, 2, 3, 5) alloys were manufactured by atmosphere controlled induction system. The effect of Ag ratio on the microstructural properties, micro-hardness, density, and homogeneity of the Mg-Ni-Y alloys were investigated. These alloys were characterized by X-ray diffraction (XRD), optical microscopy, scanning electron microscopy with energy dispersive X-ray (SEM-EDX) and Vickers micro-hardness measurement. According to XRD results, [Ni.sub.3]Y and [Mg.sub.6]Ni phases were observed as well as AgY and [Ag.sub.17][Mg.sub.54] phases, which were obtained in alloys. The quantitative results of EDX analysis confirm that the chemical composition of the obtained phases is very close and their homogeneities are so high. The average micro-hardness values of the ingot alloys were measured between 208 and 266 HV for matrix. The elastic modulus and densities of the [Mg.sub.65][Ni.sub.20][Y.sub.-15-X][Ag.sub.X] (X = 1, 2, 3, 5) alloys increased by increasing Ag in the alloys and they were determined in the range of 58.18-68.12 GPa and 3.14-3.53 g/[cm.sup.3], respectively. Key words: Mg-based alloys, micro-hardness, elastic modulus, arc melting, XRD. Nous fabriquons ici des alliages de [Mg.sub.65][Ni.sub.20][Y.sub.15-X][Ag.sub.X] (X = 1, 2, 3, 5) par un systeme a induction a controle par l'atmospherique. Nous examinons les effets du rapport d'Ag sur les proprietes microstructurales, la micro-durete et l'homogeneite des alliages Mg-Ni-Y. Nous caracterisons ces alliages par diffraction X (DX), microscopie optique, microscopie electronique a balayage avec analyse X dispersive en energie (MEB-AXD) et mesure de micro-durete Vickers. Selon les resultats DX, nous observons les phases [Ni.sub.3]Y et [Mg.sub.6]Ni, aussi bien que les phases AgY et [Ag.sub.17][Mg.sub.54] obtenues dans les alliages. Les resultats quantitatifs de l'analyse AXD confirment que la composition chimique des phases obtenues est tres proche et leurs homogeneites sont aussi grandes. Les valeurs moyennes de micro-durete des lingots sont mesurees etre entre 208 et 266 HV pour la matrice. Le module elastique et la densite des alliages de [Mg.sub.65][Ni.sub.20][Y.sub.15-X][Ag.sub.X] (X = 1, 2, 3, 5) augmentent avec la concentration de Ag dans l'alliage et sont determines etre dans les domaines 58.18-68.12 GPa et 3.14-3.53 g/[cm.sup.3] respectivement. [Traduit par la Redaction] Mots-cles: alliages bases sur du Mg, micro-durete, DX, proprietes optiques, module elastique., 1. Introduction Magnesium (Mg) is one of the most abundant elements in the Earth's crust [1] and it is mostly used for engineering applications, alloyed with other metals [2]. Mg-based [...]

Abstract: Background: Psoriasis is a multigenic and multifactorial dermatological disease linked to cardiovascular diseases. Increased levels of homocysteine in patients with psoriasis have been demonstrated in many studies. The most frequently investigated genetic defect that plays a role in homocysteine metabolism is single point substitution (C to T) located on the 677th nucleotide of the methylenetetrahydrofolate reductase gene (MTHFR). Objective: In this study, we aimed to investigate methylenetetrahydrofolate C677T polymorphism in psoriasis patients in Turkey. Methods: The study included 96 patients with psoriasis and 77 controls from southern Turkey. Methylenetetrahydrofolate C677T polymorphism was analysed using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism methods. Results: In the psoriasis group, 34 CC (35.4%), 46 CT (47.9%) and 16 TT (16.7%) genotypes were found, respectively; while in the control group, the figures were 39 (50.6%), 35 (45.5%), 3 (3.9%). Homozygote and heterozygote T alleles of methylenetetrahydrofolate C677T polymorphism were significantly higher in the psoriasis than in the control group (p=0.013). Conclusion: We firstly found a correlation between methylenetetrahydrofolate C677T polymorphism and psoriasis among the southern Turkish population.