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The increasing health effects of nuclear radiation occasioned by the enhanced human activities in the environment necessitated the need for constant investigation and assessment of radiological impact on the general populace within a confined area. Based on this, Twenty two (22) (Hand dug and motorized) well water samples were collected from various locations distributed across Jos Metropolis, Jos North LGA, Plateau State, Nigeria and analyzed for the concentration activity of these radionuclides (40K, 210Pb, 224Ra, 232Th, 238U) using radiochemical analysis technique, a high resolution gamma ray Spectrometry and a radon emanometry technique. The estimated mean concentration activity of 40K, 210Pb, 224Ra, 232Th and 238U in well water samples use as drinking water were determined. The mean concentration ranges from 1.36±0.51 Bq/l to 5.75±1.30 Bq/l. The mean concentration of 40K in well water samples ranges from 3.80±1.19 Bq/l to 2.05±0.30 Bq/l. The mean concentration of dissolved 224Ra in well water samples collected varies from 5.75±1.30 Bq/l to 1.95±0.58 Bq/l. 210Pb has an average concentration of 2.68 ± 0.80 Bq/l to 1.97±0.87 Bq/l. 232Th and 238U had average concentrations of 3.09 ± 0.57 Bq/l to 1.89±0.24 Bq/l and 5.41 ± 1.37 Bq/l to 1.36±0.51 Bq/l respectively. 210Pb and 224Ra were slightly above the recommended limits of 0.10 Bq/l and 1.00 Bq/l respectively, this can be attributed to the geological formation of the sampled area. 232Th and 238U where within the accepted standard limits of 1.00 Bq/l and 10.00 Bq/l recommended value by WHO (World Health Organization) and ICRP (International commission on radiological protection). Activity concentrations of measured radionuclides are in general decreasing in this order: 238U > 40K > 224Ra > 232Th > 210Pb for well water samples within Jos North LGA, respectively.

Background Cancers, with increasing incidence and mortality rates, constitute a leading public health problem in Nigeria. As the burden of cancer in Nigeria increases, research and quality service delivery remain critical strategies for improved cancer control across the continuum of care. This study contextualizes the challenges and gaps in oncology research and practice in Nigeria, and presents recommendations to address the gaps. Methods This qualitative study was conducted among interprofessional and interdisciplinary stakeholders in oncology healthcare practice and research in academic settings, between July and September 2021. Key-informant interviews were held with six stakeholders and leaders in nursing, pharmacy, and medicine across the six geopolitical zones of Nigeria, and twenty-four in-depth interviews with early- or mid-career researchers or healthcare professionals involved in cancer prevention and treatment were conducted. The data were analyzed using a deductive thematic analysis approach and coded using the NVIVO 12 software. Results Five sub-themes were identified as major challenges to oncology research, including poor funding, excessive workload, interprofessional rivalry, weak collaboration, and denial of cancer diagnosis by patients. Challenges identified for oncology practice were poor governance and financing, high costs of oncology treatments, poor public awareness of cancer, workforce shortage, and interprofessional conflicts. Recommended strategies for addressing these challenges were improved financing of oncology research and practice by government and relevant stakeholders, increasing interest of medical, nursing, and pharmaceutical students in oncology research through curricula-based approach and mentorship, increased oncology workforce, and improved intra- and inter-professional collaboration. Conclusion These data highlight the challenges and barriers in oncology practice and research in Nigeria, and underscore the urgent need for increased investments in infrastructure to provide interdisciplinary and interprofessional research training for high-quality care. Only then can Nigeria effectively tackle the current and impending cancer burden in the country.

Purpose: To elucidate how fluconazole and the methanol extract of Artemisia annua interact to inhibit the growth of fungi. Methods: Five hundred clinical samples of Candida albicans were isolated from patients attending three tertiary health facilities in Enugu State, Nigeria, namely, University of Nigeria Teaching Hospital (UNTH), Ituku Ozala, Bishop Shanahan Hospital (BSH), Nsukka, and Enugu State University Teaching Hospital (ESUTH), Enugu. Out of the five hundred clinical samples collected, eight genetically proven C. albicans isolates were employed for this study. The synergy between Artemisia annua and fluconazole was characterized by checkerboard analyses and fractional inhibitory concentration indices (FIC). Results: Minimum inhibitory concentration (MIC) testing results show that most isolates were resistant to selected azoles employed in the study. Based on fractional inhibitory concentration (FIC) index, specific mixtures of the extract and FLU had synergistic effects on isolates of resistant Candida albicans. Conclusion: When combined with fluconazole, the extract of A. annua demonstrated better inhibition than fluconazole alone against resistant C. albicans. This suggests a promising strategy for tackling the challenge of emerging fungal antibiotic resistance. [ABSTRACT FROM AUTHOR]

Melissa officinalis L. is used in traditional European and Iranian folk medicines to treat a plethora of neurological diseases including epilepsy. We utilized the in vitro and in vivo models of epilepsy to probe the anticonvulsant potentials of essential oil from M. officinalis (MO) to gain insight into the scientific basis for its applications in traditional medicine for the management of convulsive disorders. MO was evaluated for effects on maximal electroshock (MES) and pentylenetetrazole (PTZ) -induced seizures in mice, on 4–aminopyridine (4-AP)-brain slice model of epilepsy and sustained repetitive firing of current clamped neurons; and its ameliorative effects were examined on seizure severity, anxiety, depression, cognitive dysfunction, oxidative stress and neuronal cell loss in PTZ-kindled rats. MO reversibly blocked spontaneous ictal-like discharges in the 4-AP-brain slice model of epilepsy and secondary spikes from sustained repetitive firing, suggesting anticonvulsant effects and voltage-gated sodium channel blockade. MO protected mice from PTZ– and MES–induced seizures and mortality, and ameliorated seizure severity, fear-avoidance, depressive-like behavior, cognitive deficits, oxidative stress and neuronal cell loss in PTZ–kindled rats. The findings warrant further study for the potential use of MO and/or its constituent(s) as adjunctive therapy for epileptic patients.

BACKGROUND: This study aimed to develop a risk-scoring model for hypertension among Africans. METHODS: In this study, 4413 stroke-free controls were used to develop the risk-scoring model for hypertension. Logistic regression models were applied to 13 risk factors. We randomly split the dataset into training and testing data at a ratio of 80:20. Constant and standardized weights were assigned to factors significantly associated with hypertension in the regression model to develop a probability risk score on a scale of 0 to 1 using a logistic regression model. The model accuracy was assessed to estimate the cutoff score for discriminating hypertensives. RESULTS: Mean age was 59.9±13.3 years, 56.0% were hypertensives, and 8 factors, including diabetes, age =65 years, higher waist circumference, (BMI) =30 kg/m², lack of formal education, living in urban residence, family history of cardiovascular diseases, and dyslipidemia use were associated with hypertension. Cohen κ was maximal at =0.28, and a total probability risk score of =0.60 was adopted for both statistical weighting for risk quantification of hypertension in both datasets. The probability risk score presented a good performance--receiver operating characteristic: 64% (95% CI, 61.0-68.0), a sensitivity of 55.1%, specificity of 71.5%, positive predicted value of 70.9%, and negative predicted value of 55.8%, in the test dataset. Similarly, decision tree had a predictive accuracy of 67.7% (95% CI, 66.1-69.3) for the training set and 64.6% (95% CI, 61.0-68.0) for the testing dataset. CONCLUSIONS: The novel risk-scoring model discriminated hypertensives with good accuracy and will be helpful in the early identification of community-based Africans vulnerable to hypertension for its primary prevention. [ABSTRACT FROM AUTHOR]

In vitro and in vivo experiments were conducted to evaluate the bioactivity of nanoparticles of silver and neem (AgNeemnano) solution on some organisms that cause yam rot. In the in vitro study, organisms were isolated from a decaying yam tuber and identified as Fusarium moniliforme and Lasiodiplodia theobromae. The organisms were cultured in Sabouraud dextrose agar. With a cork borer of diameter 8 mm, cups were made at the centre of each of the agar plates, and then 0.05 ml AgNeemnano solution in varying concentrations (28.40, 56.81, 113.63, and 227.27 mg/ml) was aseptically introduced into the cups starting from the lowest to the highest concentration. The plates were incubated at 35°C for 48 h, and the zones of inhibition were determined. In the in vivo study, sterile yam slices, each weighing approximately 12.6 g, were dipped into each level of AgNeemnano solution for 3 min and thereafter inserted into the Petri dishes containing each of the organisms. The minimum inhibition concentration (MIC) of the AgNeemnano that inhibited the mycelial growth of F. moniliforme was 0.8 mg/ml, while the MIC of the AgNeemnano for L. theobromae was 0.5 mg/ml. The sterile yam slices dipped in the AgNeemnano solution and later inserted into cultured organisms were not attacked by the rot-causing organisms.

Purpose: To investigate the hematinic, anti-plasmodial potentials, and toxic effects of an aqueous extract of the leaf of Justicia secunda in mice (Mus musculus) made anemic by Plasmodium beighei. Methods: The LD50 of J. secunda was determined using Lorke’s method. Male albino mice ages 4 – 5 months, totaling forty-eight (48), were assigned at random to six groups (1 – 6). Groups 1, 2, and 3 mice were P. berghei-infected and were given extract doses of 200, 400, and 600 mg/kg of body weight, respectively. Group 4 (positive control) was infected and treated with 0.3 mL of vitamin B12 (standard). Group 5 (negative control) was infected and untreated, while group 6 (normal control) was uninfected and untreated. Blood samples were taken on days 7, 12, and 16 post-infections to measure haemoglobin (Hb), red blood cells (RBC), packed cell volume (PCV), alanine transaminase, aspartate transaminase, alkaline phosphatase, creatinine, and urea using standard methods. Results: Lower concentrations (200 and 400 mg) of the extract reduced P. berghei parasitemia. In comparison to vitamin B12, there was a negligible drop in Hb, PCV, and RBC levels in the infected groups. Both J. secunda extract and vitamin B12 did not improve the hematinic status of the mice infected with P. berghei. The hematinic condition of the mice with P. berghei infection was not improved by J. secunda extract or vitamin B12. Biochemical analysis showed no toxicity due to extract intake. Conclusion: Justicia secunda leaf extract has anti-plasmodial efficacy in P. berghei-infected mice. Studies are required to validate these findings in humans. [ABSTRACT FROM AUTHOR]

Background There is paucity of data-driven scientific reports from sub-Saharan Africa on the burden of spontaneous intracerebral haemorrhage (sICH). We have maintained a prospective consecutive in-hospital data base of cases of sICH referred for neurosurgical intervention over a 5-year period. Methods This is a cross-sectional descriptive study of the clinical epidemiology and brain-CT characterization of sICH from the data base in this region in the current era. Results There were 63 subjects, 38 (60.3%) males, aged 28 to 85 years, mean 55.7 (SD, 12.7), the modal age distribution being the sixth decade. Uncontrolled hypertension was the main predisposition in the study: present, premorbid, in 79% but uncontrolled in 88% of these known cases, and exhibited malignant derangements of blood pressure in more than half. The clinical ictus to in-hospital presentation was delayed, median 72 hours; was in severe clinical state in 70%; 57% was comatose, and was complicated with fever in 57% and respiratory morbidity also in 55.6%. The main clinical symptomatology was hemiparesis, headache, vomiting and aphasia. The sICH was supratentorial on brain CT in 85.7%, ganglionic in 50.8% and thalamic in 58.3% of the latter. The bleed had CT evidence of mass effect and intraventricular extension in more than half. Twenty three patients (36.5%) underwent operative interventionsConclusions In this patient population, sICH is mainly ganglionic and thalamic in location with significant rate of associated IVH. In-hospital clinical presentation is delayed and in critical state, and, the bleeding is uncontrolled hypertension related in >95%.