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The accurate evaluation of denitrification rate and greenhouse gas (GHG) emission in field-scale woodchip bioreactors for onsite wastewater treatment are problematic due to inevitably varied environmental conditions and underestimated GHG production with limited analysis of dissolved gas in field samples. To address these problems, batch incubation experiments were conducted with controlled conditions to precisely evaluate the denitrification kinetics and N 2 O and CH 4 emission of both gaseous and dissolved phases in fresh (6 months) and aged (5 years) woodchip bioreactors treating onsite wastewater at high (1-3 mg L -1 ) and no (0 mg L -1 ) dissolved oxygen (DO) levels. NO 3 - removal rate decreased from 37.5-119.0 g NO 3 - -N m -3 d -1 at no DO to 8.8-16.6 g NO 3 - -N m -3 d -1 at high DO (1-3 mg L -1 ) due to the growth suppression of NO 2 - reducing microorganisms (37-55 % lower nirS+nirK abundance). However, the presence of high DO increased N 2 O emission level from 5.6-6.9 mg N 2 ON m -3 at no DO to 179.5-273.6 mg N 2 ON m -3 ) due to the enhanced growth of NO reducing microorganisms (1-7 times higher norB levels) and the decreased abundance of N 2 O reducing microorganisms (53-75 % lower nosZ abundance). On the other hand, increased DO level negatively correlated with CH 4 production (1.0-3.9 g CH 4 -C m -3 d -1 ) in fresh woodchips, while showed insignificant impact on CH 4 production (0.1-1.4 g CH 4 -C m -3 d -1 ) in aged woodchips. Woodchip age increase (5 years) negatively impacted the NO 3 - removal rate (75-85 % lower than fresh woodchips) and CH 4 production rate (>3 times lower than fresh woodchips), probably due to the reduced biomass density of NO 2 - reducing microorganisms (52-58 % lower nirS+nirK abundance) and methanogens (95-98 % lower mcrA levels). The incubation results suggested that long hydraulic retention time (>2-5 days) and anaerobic/anoxic condition are preferred for the optimal NO 3 - removal and low N 2 O emission potential of woodchip bioreactors treating onsite wastewater., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Xinwei Mao reports financial support was provided by New York State Department of Environmental Conservation. Xinwei Mao reports financial support was provided by National Science Foundation. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The effectiveness of phosphorus (P) removal by sand filters is limited during septic tank effluent (STE) treatment. The elevated effluent P concentrations pose threats to drinking water quality and contribute to eutrophication. The concern of P leaching from sand filters is further exacerbated by the increased frequency of flooding and natural precipitation due to climate change. This study aimed to understand P attenuation and leaching dynamics, as well as the removal mechanisms in sand filters treating STE, offering insights into the design and implementation of P removal/recovery modules to onsite wastewater treatment systems. P attenuation and leaching during STE treatment and rainfall were studied in bench-scale columns (new vs. aged sand). At standard STE loading (1.2 gallon d -1 ft -2 ), 24-32% removal of total phosphorus (TP) was achieved, while increased P removal efficiency (35-53%) was observed at low loading (0.6 gallon d -1 ft -2 ) with influent containing 10.3-20.0 mg P L -1 . Complete breakthroughs were observed in both aged (12-70 days) and new columns (27-73 days) at test hydraulic loadings. The maximum TP attenuation level was 20.6-45.3 mg P kg -1 and 25.3-33.0 mg P kg -1 , in aged and new sand columns, respectively. When simulated rain was applied (15-60 mm h -1 ), 80-97% of the attenuated P leached out and the leaching dynamics were impacted by rainfall duration rather than the intensity. The highest concentrations of TP (15.6-15.9 mg L -1 ) were leached out from both columns within the first 2-6 h. Orthophosphate was the dominant P species in treated effluent (83-84%) and leachate (69-88%), demonstrating its significance as the major P form in the discharge. In addition, aged sand (>5 years) accumulated higher levels of Mg, Al, Ca, and Fe, thus enhancing the P attenuation level during STE treatment. Collectively, this study underscored the importance of frequent field monitoring for reliable long-term P removal estimates., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Dinophysis, a mixotrophic dinoflagellate that is known to prey on the ciliate Mesodinium rubrum, and retain its chloroplasts, is responsible for diarrhetic shellfish poisoning (DSP) in humans and has been identified on all U.S. coasts. Monocultures of Dinophysis have been used to investigate the growth of Dinophysis species in response to variations in environmental conditions, however, little is known about the roles of system stability (turbulence) and mixotrophy in the growth and toxicity of Dinophysis species in the U.S.. To begin to address this gap in knowledge, culturing experiments were conducted with three species (four strains) of Dinophysis, that included predator-prey co-incubation (Dinophysis spp.+ M. rubrum) and prey-only (M. rubrum) flasks. Cultures were investigated for effects of low or high turbulence on Dinophysis spp. growth, feeding, and amounts of intra- and extracellular toxins: okadaic acid and derivatives (diarrhetic shellfish toxins, DSTs) and pectenotoxins (PTXs). Turbulence did not have a measurable effect on the rates of ingestion of M. rubrum prey by Dinophysis spp. for any of the four strains, however, effects on growth and particulate and dissolved toxins were observed. High turbulence (ε = 10 -2 m 2 s -3 ) significantly slowed growth of both D. acuminata and D. ovum relative to still controls, but significantly stimulated growth of the D. caudata strain. Increasing turbulence also resulted in significantly higher intracellular toxin content in D. acuminata cultures (DSTs and PTXs), but significantly reduced intracellular toxin content (PTXs) in those of D. caudata. An increase in turbulence appeared to promote toxin leakage, as D. ovum had significantly more extracellular DSTs found in the medium under high turbulence when compared to the still control. Overall, significant responses to turbulence were observed, whereby the three strains from the "Dinophysis acuminata complex" displayed a stress response to turbulence, i.e., decreasing growth, increasing intracellular toxin content and/or increasing toxin leakage, while the D. caudata strain had an opposite response, appearing stimulated by, or more tolerant of, high turbulence., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

This study aimed to explore the effects of different light intensities on the ecophysiology of eight new Dinophysis isolates comprising four species (D. acuminata, D. ovum, D. fortii, and D. caudata) collected from different geographical regions in the US. After six months of acclimation, the growth rates, photosynthetic efficiency (F v /F m ratio), toxin content, and net toxin production rates of the Dinophysis strains were examined. The growth rates of D. acuminata and D. ovum isolates were comparable across light intensities, with the exception of one D. acuminata strain (DANY1) that was unable to grow at the lowest light intensity. However, D. fortii and D. caudata strains were photoinhibited and grew at a slower rate at the highest light intensity, indicating a lower degree of adaptability and tolerance to such conditions. Photosynthetic efficiency was similar for all Dinophysis isolates and negatively correlated with exposure to high light intensities. Multiple toxin metrics, including cellular toxin content and net production rates of DSTs and PTXs, were variable among species and even among isolates of the same species in response to light intensity. A pattern was detected, however, whereby the net production rates of PTXs were significantly lower across all Dinophysis isolates when exposed to the lowest light intensity. These findings provide a basis for understanding the effects of light intensity on the eco-physiological characteristics of Dinophysis species in the US and could be employed to develop integrated physical-biological models for species and strains of interest to predict their population dynamics and mitigate their negative effects., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Seaweeds are ecosystem engineers that can serve as habitat, sequester carbon, buffer ecosystems against acidification, and, in an aquaculture setting, represent an important food source. One health issue regarding the consumption of seaweeds and specifically, kelp, is the accumulation of some trace elements of concern within tissues. As atmospheric CO 2 concentrations rise, and global oceans acidify, the concentrations of elements in seawater and kelp may change. Here, we cultivated the sugar kelp, Saccharina latissima under ambient (~400 μatm) and elevated pCO 2 (600-2400 μatm) conditions and examined the accumulation of trace elements using x-ray powder diffraction, sub-micron resolution x-ray imaging, and inductively coupled plasma mass spectrometry. Exposure of S. latissima to higher concentrations of pCO 2 and lower pH caused a significant increase (p < 0.05) in the iodine and arsenic content of kelp along with increased subcellular heterogeneity of these two elements as well as bromine. The iodine-to‑calcium and bromine-to‑calcium ratios of kelp also increased significantly under high CO 2 /low pH (p < 0.05). In contrast, high CO 2 /low pH significantly reduced levels of copper and cadmium in kelp tissue (p < 0.05) and there were significant inverse correlations between concentrations of pCO 2 and concentrations of cadmium and copper in kelp (p < 0.05). Changes in copper and cadmium levels in kelp were counter to expected changes in their free ionic concentrations in seawater, suggesting that the influence of low pH on algal physiology was an important control on the elemental content of kelp. Collectively, these findings reveal the complex effects of ocean acidification on the elemental composition of seaweeds and indicate that the elemental content of seaweeds used as food must be carefully monitored as climate change accelerates this century., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Background: The application of wastewater-based epidemiology to track the outbreak and prevalence of coronavirus disease (COVID-19) in communities has been tested and validated by several researchers across the globe. However, the RNA-based surveillance has its inherent limitations and uncertainties., Objective: This study aims to complement the ongoing wastewater surveillance efforts by analyzing other chemical biomarkers in wastewater to help assess community response (hospitalization and treatment) during the pandemic (2020-2021)., Methods: Wastewater samples (n = 183) were collected from the largest wastewater treatment facility in Suffolk County, NY, USA and analyzed for COVID-19 treatment drugs (remdesivir, chloroquine, and hydroxychloroquine (HCQ)) and their human metabolites. We additionally monitored 26 pharmaceuticals including common over-the-counter (OTC) drugs. Lastly, we developed a Bayesian model that uses viral RNA, COVID-19 treatment drugs, and pharmaceuticals data to predict the confirmed COVID-19 cases within the catchment area., Results: The viral RNA levels in wastewater tracked the actual COVID-19 case numbers well as expected. COVID-19 treatment drugs were detected with varying frequency (9-100%) partly due to their instability in wastewater. We observed a significant correlation (R = 0.30, p < 0.01) between the SARS-CoV-2 genes and desethylhydroxychloroquine (DHCQ, metabolite of HCQ). Remdesivir levels peaked immediately after the Emergency Use Authorization approved by the FDA. Although, 13 out of 26 pharmaceuticals assessed were consistently detected (DF = 100%, n = 111), only acetaminophen was significantly correlated with viral loads, especially when the Omicron variant was dominant. The Bayesian models were capable of reproducing the temporal trend of the confirmed cases., Impact: In this study, for the first time, we measured COVID-19 treatment and pharmaceutical drugs and their metabolites in wastewater to complement ongoing COVID-19 viral RNA surveillance efforts. Our results highlighted that, although the COVID-19 treatment drugs were not very stable in wastewater, their detection matched with usage trends in the community. Acetaminophen, an OTC drug, was significantly correlated with viral loads and confirmed cases, especially when the Omicron variant was dominant. A Bayesian model was developed which could predict COVID-19 cases more accurately when incorporating other drugs data along with viral RNA levels in wastewater., (© 2023. The Author(s).)

Warmer temperatures can significantly increase the intensity of cyanobacterial harmful algal blooms (CHABs) in eutrophic freshwater ecosystems. However, few studies have examined the effects of CO 2 enrichment in tandem with elevated temperature and/or nutrients on cyanobacterial taxa in freshwater ecosystems. Here, we observed changes in the biomass of cyanobacteria, nutrients, pH, and carbonate chemistry over a two-year period in a shallow, eutrophic freshwater lake and performed experiments to examine the effects and co-effects of CO 2 , temperature, and nutrient enrichment on cyanobacterial and N 2 -fixing (diazotrophic) communities assessed via high throughput sequencing of the 16S rRNA and nifH genes, respectively. During both years, there were significant CHABs (50-500 μg cyanobacterial chlorophyll-a L -1 ) and lake CO 2 levels were undersaturated (≤300 μatm pCO 2 ). NH 4 + significantly increased the net growth rates of cyanobacteria as well as the biomass of the diazotrophic cyanobacterial order Nostocales under elevated and ambient CO 2 conditions. In a fall experiment, the N 2 fixation rates of Nostocales were significantly higher when populations were enriched with CO 2 and P, relative to CO 2 -enriched populations that were not amended with P. During a summer experiment, N 2 fixation rates increased significantly under N and CO 2 - enriched conditions relative to N-enriched and ambient CO 2 conditions. Nostocales dominated the diazotrophic communities of both experiments, achieving the highest relative abundance under CO 2 -enriched conditions when N was added in the first experiment and when CO 2 and temperature were elevated in the second experiment, when N 2 fixation rates also increased significantly. Collectively, this study indicates that N promotes cyanobacterial blooms including those formed by Dolichospermum and that the biomass and N 2 fixation rates of diazotrophic cyanobacterial taxa may benefit from enhanced CO 2 levels in eutrophic lakes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Aureococcus anophagefferens is a small marine pelagophyte that forms recurrent harmful brown tides blooms with adverse ecological and economic impacts. During blooms, A. anophagefferens experiences lower zooplankton grazing mortality than other phytoplankton potentially due to the synthesis of anti-predator compounds including extracellular polysaccharides. This study characterized the transcriptomic response of A. anophagefferens when exposed to the protozooplankton, Oxyrrhis marina , and assessed whether this response involved chemical cues. Transcriptomes were generated from A. anophagefferens populations grown at high (1×10 6 cells mL -1 ) and low (5×10 5 cells mL -1 ) cell densities incubated directly with O. marina or receiving only filtrate from co-cultures of A. anophagefferens and O. marina to evaluate the role of chemical cues. There were a greater number of genes differentially expressed in response to grazing in the lower concentration of A. anophagefferens compared to the high concentration treatment and in response to direct grazing compared to filtrate. KEGG pathway analysis revealed that direct grazer exposure led to a significant increase in transcripts of genes encoding secondary metabolite production ( p  < 0.001). There was broad transcriptional evidence indicating the induction of biosynthetic pathways for polyketides and sterols in response to zooplankton grazers, compounds associated with damage to marine organisms. In addition, exposure to O. marina elicited changes in the abundance of transcripts associated with carbohydrate metabolism that could support the formation of an extracellular polysaccharide matrix including genes related to glycoprotein synthesis and carbohydrate transport. Collectively, these findings support the hypothesis that A. anophagefferens can induce biochemical pathways that reduce grazing mortality and support blooms., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Dawydiak and Gobler.)

Globally, oyster reef restoration is one of the most widely applied coastal restoration interventions. While reefs are focal points of processes tightly linked to the carbonate system such as shell formation and respiration, how these processes alter reef carbonate chemistry relative to the surrounding seawater is unclear. Moreover, coastal systems are increasingly impacted by coastal acidification, which may affect reef carbonate chemistry. Here, we characterized the growth of multiple constructed reefs as well as summer variations in pH and carbonate chemistry of reef-influenced seawater (in the middle of reefs) and ambient seawater (at locations ~50 m outside of reefs) to determine how reef chemistry was altered by the reef community and, in turn, impacts resident oysters. High frequency monitoring across three subtidal constructed reefs revealed reductions of daily mean and minimum pH (by 0.05-0.07 and 0.07-0.12 units, respectively) in seawater overlying reefs relative to ambient seawater (p < .0001). The proportion of pH measurements below 7.5, a threshold shown to negatively impact post-larval oysters, were 1.8×-5.2× higher in reef seawater relative to ambient seawater. Most reef seawater samples (83%) were reduced in total alkalinity relative to ambient seawater samples, suggesting community calcification was a key driver of modified carbonate chemistry. The net metabolic influence of the reef community resulted in reductions of CaCO 3 saturation state in 78% of discrete samples, and juvenile oysters placed on reefs exhibited slower shell growth (p < .05) compared to oysters placed outside of reefs. While differences in survival were not detected, reef oysters may benefit from enhanced survival or recruitment at the cost of slowed growth rates. Nevertheless, subtidal restored reef communities modified seawater carbonate chemistry in ways that likely increased oyster vulnerability to acidification, suggesting that carbonate chemistry dynamics warrant consideration when determining site suitability for oyster restoration, particularly under continued climate change., (© 2023 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)

Since the discovery of the first "giant virus," particular attention has been paid toward isolating and culturing these large DNA viruses through Acanthamoeba spp. bait systems. While this method has allowed for the discovery of plenty novel viruses in the Nucleocytoviricota , environmental -omics-based analyses have shown that there is a wealth of diversity among this phylum, particularly in marine datasets. The prevalence of these viruses in metatranscriptomes points toward their ecological importance in nutrient turnover in our oceans and as such, in depth study into non-amoebal Nucleocytoviricota should be considered a focal point in viral ecology. In this review, we report on Kratosvirus quantuckense (née Aureococcus anophagefferens Virus), an algae-infecting virus of the Imitervirales . Current systems for study in the Nucleocytoviricota differ significantly from this virus and its relatives, and a litany of trade-offs within physiology, coding potential, and ecology compared to these other viruses reveal the importance of K. quantuckense . Herein, we review the research that has been performed on this virus as well as its potential as a model system for algal-virus interactions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Truchon, Chase, Gann, Moniruzzaman, Creasey, Aylward, Xiao, Gobler and Wilhelm.)

Allelopathy is a biological mechanism that can promote harmful algal blooms (HAB) via the inhibition of sympatric phytoplankton. While nutrient loading can also promote HABs, the ability of allelopathy to stimulate HABs via the regeneration of nutrients has yet to be explored. To examine the impacts of allelopathically liberated N on HAB species, a series of experiments were performed using multiple allelopathic HAB species including the dinoflagellates Alexandrium catenella and Margalefidinium polykrikoides, and the pelagophyte, Aureoumbra lagunensis. These HAB species were paired with the cosmopolitan dinoflagellate, Akashiwo sanguinea, that was labeled with 15 NO 3 - or 15 NH 4 + , allowing the release and transfer of N to be traced as a time course during allelopathic interactions. During all experiments, the allelopathic inhibition of Akashiwo was accompanied by increases in cell densities, growth rates, and the δ 15 N content of the HAB species, evidencing the transfer of N liberated from Akashiwo. The cellular transfer of 15 N and release of dissolved N was higher when Akashiwo was grown with 15 NO 3 - compared to 15 NH 4 + suggesting a differential subcellular-compartmentalization of N sources. Regardless of the type of N, HAB species obtained 60 - 100% of their cellular N from lysed Akashiwo cells and there was an enrichment of the δ 15 N content of the dissolved NH 4 + pool post-lysis of Akashiwo. Collectively, the results demonstrate that beyond facilitating species succession, allelopathy can supply HABs with N and, therefore, is likely important for promoting and sustaining HABs. Given that allelopathy is known to be a dose-dependent process, allelopathy may induce a positive feedback loop, whereby competitors are lysed, N is liberated, HABs are intensified and, in turn, become more strongly allelopathic., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

The impacts of harmful algal blooms (HABs) on economies, public health, ecosystems, and aquaculture across the globe have all increased in recent decades, and this has been acutely the case in China. Here, we review the history of HABs and HABs research in China, as well as recent trends in HABs and future prospects of HAB science in China. The most updated analyses demonstrated that the number of HAB events, the number of HAB species, the aerial coverage of HABs, and the impacts of HABs in Chinese waters during the 21 st century were all higher than that during the last two decades of the 20 th century. The increase in the number of HABs in China has been significantly correlated with the increased discharge of ammonium and total phosphorus into coastal waters (p < 0.01 for both). Notable newly recognized events this century have included chronic HABs caused by Prorocentrum donghaiense and Karenia mikimotoi, a paralytic shellfish poisoning event caused by Gymnodinium catenatum that sickened 80 people, brown tides caused by Aureococcus anophagefferens, green tides caused by Ulva prolifera, golden tides caused by Sargassum horneri, and the disruption of a nuclear power plant caused by a bloom of Phaeocystis globosa. A series of key discoveries regarding HABs has been made this century including documentation of nearly all known HAB toxins in Chinese waters, discovery of novel cyst-formation and/or life stages of multiple HABs-causing species, identification of the chemical and physical oceanographic drivers of multiple HABs including those formed by P. donghaiense, K. mikimotoi, and U. prolifera, and the successful mitigation of HABs via the use of modified clay approaches. Future research prospects highlighted include the use of macroalgae as a means to prevent, mitigate, and control (PCM) HABs and the process by which multi-disciplinary studies involving molecular approaches (omics), remote in situ detection, artificial intelligence, and mega-data analyses might be used to develop refined and realistic HAB forecasting platforms. Collectively, this review demonstrates the significant evolution of HAB science since the 20 th century in China and demonstrates that while HABs in China are complex and widespread, recent and on-going discoveries make the development of detailed understanding and effective measures to mitigate the negative effects of HABs a hopeful outcome in the coming years., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

Background: The public health response to COVID-19 has shifted to reducing deaths and hospitalizations to prevent overwhelming health systems. The amount of SARS-CoV-2 RNA fragments in wastewater are known to correlate with clinical data including cases and hospital admissions for COVID-19. We developed and tested a predictive model for incident COVID-19 hospital admissions in New York State using wastewater data., Methods: Using county-level COVID-19 hospital admissions and wastewater surveillance covering 13.8 million people across 56 counties, we fit a generalized linear mixed model predicting new hospital admissions from wastewater concentrations of SARS-CoV-2 RNA from April 29, 2020 to June 30, 2022. We included covariates such as COVID-19 vaccine coverage in the county, comorbidities, demographic variables, and holiday gatherings., Findings: Wastewater concentrations of SARS-CoV-2 RNA correlated with new hospital admissions per 100,000 up to ten days prior to admission. Models that included wastewater had higher predictive power than models that included clinical cases only, increasing the accuracy of the model by 15%. Predicted hospital admissions correlated highly with observed admissions (r = 0.77) with an average difference of 0.013 hospitalizations per 100,000 (95% CI = [0.002, 0.025])., Interpretation: Using wastewater to predict future hospital admissions from COVID-19 is accurate and effective with superior results to using case data alone. The lead time of ten days could alert the public to take precautions and improve resource allocation for seasonal surges., Competing Interests: The authors declare that they have not competing financial interests., (© 2023 The Authors.)

Multiple species of the genus Dinophysis produce diarrhetic shellfish toxins (okadaic acid and Dinophysis toxins, OA/DTXs analogs) and/or pectenotoxins (PTXs). Only since 2008 have DSP events (illnesses and/or shellfish harvesting closures) become recognized as a threat to human health in the United States. This study characterized 20 strains representing five species of Dinophysis spp. isolated from three US coastal regions that have experienced DSP events: the Northeast/Mid-Atlantic, the Gulf of Mexico, and the Pacific Northwest. Using a combination of morphometric and DNA-based evidence, seven Northeast/Mid-Atlantic isolates and four Pacific Northwest isolates were classified as D. acuminata, a total of four isolates from two coasts were classified as D. norvegica, two isolates from the Pacific Northwest coast were identified as D. fortii, and three isolates from the Gulf of Mexico were identified as D. ovum and D. caudata. Toxin profiles of D. acuminata and D. norvegica varied by their geographical origin within the United States. Cross-regional comparison of toxin profiles was not possible with the other three species; however, within each region, distinct species-conserved profiles for isolates of D. fortii, D. ovum, and D. caudata were observed. Historical and recent data from various State and Tribal monitoring programs were compiled and compared, including maximum recorded cell abundances of Dinophysis spp., maximum concentrations of OA/DTXs recorded in commercial shellfish species, and durations of harvesting closures, to provide perspective regarding potential for DSP impacts to regional public health and shellfish industry., (© 2023 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America.)

Warming temperatures and diminishing dissolved oxygen (DO) concentrations are among the most pervasive drivers of global coastal change. While regions of the Northwest Atlantic Ocean are experiencing greater than average warming, the combined effects of thermal and hypoxic stress on marine life in this region are poorly understood. Populations of the northern bay scallop, Argopecten irradians irradians across the northeast United States have experienced severe declines in recent decades. This study used a combination of high-resolution (~1 km) satellite-based temperature records, long-term temperature and DO records, field and laboratory experiments, and high-frequency measures of scallop cardiac activity in an ecosystem setting to quantify decadal summer warming and assess the vulnerability of northern bay scallops to thermal and hypoxic stress across their geographic distribution. From 2003 to 2020, significant summer warming (up to ~0.2°C year -1 ) occurred across most of the bay scallop range. At a New York field site in 2020, all individuals perished during an 8-day estuarine heatwave that coincided with severe diel-cycling hypoxia. Yet at a Massachusetts site with comparable DO levels but lower daily mean temperatures, mortality was not observed. A 96-h laboratory experiment recreating observed daily temperatures of 25 or 29°C, and normoxia or hypoxia (22.2% air saturation), revealed a 120-fold increased likelihood of mortality in the 29°C-hypoxic treatment compared with control conditions, with scallop clearance rates also reduced by 97%. Cardiac activity measurements during a field deployment indicated that low DO and elevated daily temperatures modulate oxygen consumption rates and likely impact aerobic scope. Collectively, these findings suggest that concomitant thermal and hypoxic stress can have detrimental effects on scallop physiology and survival and potentially disrupt entire fisheries. Recovery of hypoxic systems may benefit vulnerable fisheries under continued warming., (© 2023 The Authors. Global Change Biology published by John Wiley & Sons Ltd.)