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Background: The study highlights the significance of data availability in molecular genetic research, focusing on cattle nodular dermatitis virus. Limited data on Russian genetic variants hinders comprehensive virus characterization. Molecular genetic methods are crucial for accurate diagnosis, considering cross-reactions with poxviruses. Increased data availability is essential to improve diagnostics and biosafety in the region. Method: The researchers conducted a molecular genetic analysis of the gene sequences of the nodular dermatitis virus to assess the variability of the virus. The authors used the GenBank database to compare sequences and used the MEGA X program for phylogenetic analysis and sequence alignment. Results: The sequences of the nodular dermatitis virus closest to the Russian genetic variants have been determined. The volumes of available epizootiological and molecular genetic data were compared, and the circulation of the vaccine strain was confirmed. Conclusion: The article emphasizes the need to increase the availability of data on the genetic sequences of Russian samples to fully characterize the genetic diversity of the nodular dermatitis virus. Despite the limited data, the authors observed a tendency to increase genetic diversity. They recommend isolating and storing the virus in the cell culture to enhance genetic information. [ABSTRACT FROM AUTHOR]

The objects of study were microorganisms, isolated from pathological material of chicken breeds "RoSS 208" and "Luong Phuong"; ducklings breeds "C. V. super M" and a "Khaki Campbell"; quails breed "Coturnix". In 499 samples of the birds material we discovered 153 microorganisms cultures of the genus Salmonella, which were 30.66 % of the total number of selected microorganisms cultures, including S. typhimurium – 60 (39.22 %) strains; S. albany – 32 (of 20.92 %); S. enteritidis – 20 (13.07 %); S. hadar – 9 (5.88%); S. agona and S. thompson – 5 (3.27 %); S. indiana – 4 (2.61 %); S. heidelberg, S. mbandaka and S. shalkwijk – 3 (1.96 %); S. give – 2 (1.31 %); S. derby and S. havana – 1 (0.65 %); other serovar – 5 (3.27 %). It was found that 70.0 % of the studied strains were sensitive to drugs of the fluoroquinolone group (nalidixic acid, norfloxacin, ciprofloxacin, enrofloxacin), 66.67 % – cephalosporins (ceftazidim). 83.33 % of strains were resistant to tetracycline drugs (tetracycline); 63.33 % - β- lactams (ampicillin); 56.67 % - aminoglycosides (gentamicin, kanamycin, streptomycin); 46.67 % - sulfonamides (trimethoprim). The minimum inhibitory concentration ("MIC") were established at the intersection of the growth suppression zone and the test strip "E-test": ampicillin-512 μg/ml; tetracycline – 64 μg/ml; gentamicin – 2 μg/ml; ciprofloxacin – 1 μg/ml; norfloxacin – 0.38 μg/ml and ceftazidim – 0.19 μg/ml. The amplitude values ranged from 0.019 μg/ml to 512 μg/ml [ABSTRACT FROM AUTHOR]