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46 results on '"Gmyl, Anatoly"'

1. Phlebovirus sequences detected in ticks collected in Russia: Novel phleboviruses, distinguishing criteria and high tick specificity

Author

Klimentov, Alexander S., Belova, Oxana A., Kholodilov, Ivan S., Butenko, Alexander M., Bespyatova, Liubov A., Bugmyrin, Sergey V., Chernetsov, Nikita, Ivannikova, Anna Y., Kovalchuk, Irina V., Nafeev, Alexander A., Oorzhak, Natalia D., Pilikova, Olga M., Polienko, Alexandra E., Purmak, Kristina A., Romanenko, Evgeniya N., Romanova, Lidiya Iu., Saryglar, Anna A., Solomashchenko, Nataliya I., Shamsutdinov, Anton F., Vakalova, Elena V., Lukashev, Alexander N., Karganova, Galina G., and Gmyl, Anatoly P.

Published
2020
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3. Ixodid ticks and tick-borne encephalitis virus prevalence in the South Asian part of Russia (Republic of Tuva)

Author

Kholodilov, Ivan, Belova, Oxana, Burenkova, Ludmila, Korotkov, Yuri, Romanova, Lidiya, Morozova, Lola, Kudriavtsev, Vitalii, Gmyl, Larissa, Belyaletdinova, Ilmira, Chumakov, Alexander, Chumakova, Natalia, Dargyn, Oyumaa, Galatsevich, Nina, Gmyl, Anatoly, Mikhailov, Mikhail, Oorzhak, Natalia, Polienko, Alexandra, Saryglar, Anna, Volok, Viktor, Yakovlev, Alexander, and Karganova, Galina

Published
2019
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5. Recombinant TBEV Protein E of the Siberian Subtype Is a Candidate Antigen in the ELISA Test System for Differential Diagnosis

Author

Baryshnikova, Victoria, primary, Turchenko, Yuriy, additional, Tuchynskaya, Ksenia, additional, Belyaletdinova, Ilmira, additional, Butenko, Alexander, additional, Dereventsova, Alena, additional, Ignatiev, Georgy, additional, Kholodilov, Ivan, additional, Larichev, Victor, additional, Lyapeykova, Ekaterina, additional, Rogova, Anastasiya, additional, Shakaryan, Armen, additional, Shishova, Anna, additional, Gmyl, Anatoly, additional, and Karganova, Galina, additional

Published
2023
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6. Comprehensive Elucidation of the Role of L and 2A Security Proteins on Cell Death during EMCV Infection.

Author

Ivin, Yury, Butusova, Anna, Gladneva, Ekaterina, Gmyl, Anatoly, and Ishmukhametov, Aydar

Subjects
CELL death, HELA cells, PROTEINS, VIRAL replication, CELL lines
Abstract

The EMCV L and 2A proteins are virulence factors that counteract host cell defense mechanisms. Both L and 2A exhibit antiapoptotic properties, but the available data were obtained in different cell lines and under incomparable conditions. This study is aimed at checking the role of these proteins in the choice of cell death type in three different cell lines using three mutants of EMCV lacking functional L, 2A, and both proteins together. We have found that both L and 2A are non-essential for viral replication in HeLa, BHK, and RD cell lines, as evidenced by the viability of the virus in the absence of both functional proteins. L-deficient infection led to the apoptotic death of HeLa and RD cells, and the necrotic death of BHK cells. 2A-deficient infection induced apoptosis in BHK and RD cells. Infection of HeLa cells with the 2A-deficient mutant was finalized with exclusive caspase-dependent death with membrane permeabilization, morphologically similar to pyroptosis. We also demonstrated that inactivation of both proteins, along with caspase inhibition, delayed cell death progression. The results obtained demonstrate that proteins L and 2A play a critical role in choosing the path of cell death during infection, but the result of their influence depends on the properties of the host cells. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Evolutionary origins of hepatitis A virus in small mammals

Author

the Hepatovirus Ecology Consortium, Drexler, Jan Felix, Corman, Victor M., Lukashev, Alexander N., van den Brand, Judith M. A., Gmyl, Anatoly P., Brünink, Sebastian, Rasche, Andrea, Seggewiβ, Nicole, Feng, Hui, Leijten, Lonneke M., Vallo, Peter, Kuiken, Thijs, Dotzauer, Andreas, Ulrich, Rainer G., Lemon, Stanley M., and Drosten, Christian

Published
2015

15. Isolation and Characterisation of Alongshan Virus in Russia

Author

Kholodilov, Ivan S., primary, Litov, Alexander G., additional, Klimentov, Alexander S., additional, Belova, Oxana A., additional, Polienko, Alexandra E., additional, Nikitin, Nikolai A., additional, Shchetinin, Alexey M., additional, Ivannikova, Anna Y., additional, Bell-Sakyi, Lesley, additional, Yakovlev, Alexander S., additional, Bugmyrin, Sergey V., additional, Bespyatova, Liubov A., additional, Gmyl, Larissa V., additional, Luchinina, Svetlana V., additional, Gmyl, Anatoly P., additional, Gushchin, Vladimir A., additional, and Karganova, Galina G., additional

Published
2020
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16. First isolation and characterisation of Alongshan virus in Russia

Author

Kholodilov, Ivan S., primary, Litov, Alexander G., additional, Klimentov, Alexander S., additional, Belova, Oxana A., additional, Polienko, Alexandra E., additional, Nikitin, Nikolai A., additional, Shchetinin, Alexey M., additional, Ivannikova, Anna Y., additional, Bell-Sakyi, Lesley, additional, Yakovlev, Alexander S., additional, Bugmyrin, Sergey V., additional, Bespyatova, Liubov A., additional, Gmyl, Larissa V., additional, Luchinina, Svetlana V., additional, Gmyl, Anatoly P., additional, Gushchin, Vladimir A., additional, and Karganova, Galina G., additional

Published
2019
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18. Independent evolution of tetraloop in enterovirus oriL replicative element and its putative binding partners in protein 3C

Author

Prostova, Maria A, Deviatkin, Andrei A, Tcelykh, Irina O, Lukashev, Alexander N, and Gmyl, Anatoly P

Abstract

Background. Enteroviruses are small non-enveloped viruses with (+) ssRNA genome with one open reading frame. Enterovirus protein 3C (or 3CD for some species) binds the replicative element oriL to initiate replication. The replication of enteroviruses features low fidelity, which allows the virus to adapt to the changing environment on the one hand, and requires additional mechanisms to maintain the genome stability on the other. Structural disturbances in the apical region of oriL domain d can be compensated by amino acid substitutions in positions 154 or 156 of 3C (amino acid numeration corresponds to poliovirus 3C), thus suggesting the co-evolution of these interacting sequences in nature. The aim of this work was to understand co-evolution patterns of two interacting replication machinery elements in enteroviruses, the apical region of oriL domain d and its putative binding partners in the 3C protein. Methods.To evaluate the variability of the domain d loop sequence we retrieved all available full enterovirus sequences (>6400 nucleotides), which were present in the NCBI database on February 2017 and analysed the variety and abundance of sequences in domain d of the replicative element oriL and in the protein 3C. Results.A total of 2,842 full genome sequences was analysed. The majority of domain d apical loops were tetraloops, which belonged to consensus YNHG (Y=U/C, N=any nucleotide, H=A/C/U). The putative RNA-binding tripeptide 154-156 (Enterovirus C 3C protein numeration) was less diverse than the apical domain d loop region and, in contrast to it, was species-specific. Discussion. Despite the suggestion that the RNA-binding tripeptide interacts with the apical region of domain d, they evolve independently in nature. Together, our data indicate the plastic evolution of both interplayers of 3C-oriL recognition.

Published
2017
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21. Pressure for Pattern-Specific Intertypic Recombination between Sabin Polioviruses: Evolutionary Implications

Author

Korotkova, Ekaterina, primary, Laassri, Majid, additional, Zagorodnyaya, Tatiana, additional, Petrovskaya, Svetlana, additional, Rodionova, Elvira, additional, Cherkasova, Elena, additional, Gmyl, Anatoly, additional, Ivanova, Olga, additional, Eremeeva, Tatyana, additional, Lipskaya, Galina, additional, Agol, Vadim, additional, and Chumakov, Konstantin, additional

Published
2017
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26. A Cluster of Paralytic Poliomyelitis Cases Due to Transmission of Slightly Diverged Sabin 2 Vaccine Poliovirus

Author

Korotkova, Ekaterina A., primary, Gmyl, Anatoly P., additional, Yakovenko, Maria L., additional, Ivanova, Olga E., additional, Eremeeva, Tatyana P., additional, Kozlovskaya, Liubov I., additional, Shakaryan, Armen K., additional, Lipskaya, Galina Y., additional, Parshina, Irina L., additional, Loginovskikh, Nataliya V., additional, Morozova, Nadezhda S., additional, and Agol, Vadim I., additional

Published
2016
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28. Highly Divergent Hepaciviruses from African Cattle

Author

Corman, Victor Max, primary, Grundhoff, Adam, additional, Baechlein, Christine, additional, Fischer, Nicole, additional, Gmyl, Anatoly, additional, Wollny, Robert, additional, Dei, Dickson, additional, Ritz, Daniel, additional, Binger, Tabea, additional, Adankwah, Ernest, additional, Marfo, Kwadwo Sarfo, additional, Annison, Lawrence, additional, Annan, Augustina, additional, Adu-Sarkodie, Yaw, additional, Oppong, Samuel, additional, Becher, Paul, additional, Drosten, Christian, additional, and Drexler, Jan Felix, additional

Published
2015
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29. Evidence for novel hepaciviruses in rodents

Author

Drexler, Jan Felix, Corman, Victor Max, Müller, Marcel Alexander, Lukashev, Alexander N., Gmyl, Anatoly, Coutard, Bruno, Adam, Alexander, Ritz, Daniel, Leijten, Lonneke M., Van Riel, Debby, Kallies, Rene, Klose, Stefan M., Gloza-Rausch, Florian, Binger, Tabea, Annan, Augustina, Adu-Sarkodie, Yaw, Oppong, Samuel, Bourgarel, Mathieu, Rupp, Daniel, Hoffmann, Bernd, Schlegel, Mathias, Kümmerer, Beate M., Krüger, Detlev H., Schmidt-chanasit, Jonas, Aguilar Setién, Alvaro, Cottontail, Veronika M., Hemachudha, Thiravat, Wacharapluesadee, Supaporn, Osterrieder, Klaus, Bartenschlager, Ralf, Matthee, Sonja, Beer, Martin, Kuilen, Thijs, Reusken, Chantal, Leroy, Eric M., Ulrich, Rainer G., Drosten, Christian, Drexler, Jan Felix, Corman, Victor Max, Müller, Marcel Alexander, Lukashev, Alexander N., Gmyl, Anatoly, Coutard, Bruno, Adam, Alexander, Ritz, Daniel, Leijten, Lonneke M., Van Riel, Debby, Kallies, Rene, Klose, Stefan M., Gloza-Rausch, Florian, Binger, Tabea, Annan, Augustina, Adu-Sarkodie, Yaw, Oppong, Samuel, Bourgarel, Mathieu, Rupp, Daniel, Hoffmann, Bernd, Schlegel, Mathias, Kümmerer, Beate M., Krüger, Detlev H., Schmidt-chanasit, Jonas, Aguilar Setién, Alvaro, Cottontail, Veronika M., Hemachudha, Thiravat, Wacharapluesadee, Supaporn, Osterrieder, Klaus, Bartenschlager, Ralf, Matthee, Sonja, Beer, Martin, Kuilen, Thijs, Reusken, Chantal, Leroy, Eric M., Ulrich, Rainer G., and Drosten, Christian

Abstract

Hepatitis C virus (HCV) is among the most relevant causes of liver cirrhosis and hepatocellular carcinoma. Research is complicated by a lack of accessible small animal models. The systematic investigation of viruses of small mammals could guide efforts to establish such models, while providing insight into viral evolutionary biology. We have assembled the so-far largest collection of small-mammal samples from around the world, qualified to be screened for bloodborne viruses, including sera and organs from 4,770 rodents (41 species); and sera from 2,939 bats (51 species). Three highly divergent rodent hepacivirus clades were detected in 27 (1.8%) of 1,465 European bank voles (Myodes glareolus) and 10 (1.9%) of 518 South African four-striped mice (Rhabdomys pumilio). Bats showed anti-HCV immunoblot reactivities but no virus detection, although the genetic relatedness suggested by the serologic results should have enabled RNA detection using the broadly reactive PCR assays developed for this study. 210 horses and 858 cats and dogs were tested, yielding further horseassociated hepaciviruses but none in dogs or cats. The rodent viruses were equidistant to HCV, exceeding by far the diversity of HCV and the canine/equine hepaciviruses taken together. Five full genomes were sequenced, representing all viral lineages. Salient genome features and distance criteria supported classification of all viruses as hepaciviruses. Quantitative RT-PCR, RNA in-situ hybridisation, and histopathology suggested hepatic tropism with liver inflammation resembling hepatitis C. Recombinant serology for two distinct hepacivirus lineages in 97 bank voles identified seroprevalence rates of 8.3 and 12.4%, respectively. Antibodies in bank vole sera neither cross-reacted with HCV, nor the heterologous bank vole hepacivirus. Co-occurrence of RNA and antibodies was found in 3 of 57 PCR-positive bank vole sera (5.3%). Our data enable new hypotheses regarding HCV evolution and encourage efforts to develo

Published
2013

31. Evidence for Novel Hepaciviruses in Rodents

Author

Drexler, Jan Felix, primary, Corman, Victor Max, additional, Müller, Marcel Alexander, additional, Lukashev, Alexander N., additional, Gmyl, Anatoly, additional, Coutard, Bruno, additional, Adam, Alexander, additional, Ritz, Daniel, additional, Leijten, Lonneke M., additional, van Riel, Debby, additional, Kallies, Rene, additional, Klose, Stefan M., additional, Gloza-Rausch, Florian, additional, Binger, Tabea, additional, Annan, Augustina, additional, Adu-Sarkodie, Yaw, additional, Oppong, Samuel, additional, Bourgarel, Mathieu, additional, Rupp, Daniel, additional, Hoffmann, Bernd, additional, Schlegel, Mathias, additional, Kümmerer, Beate M., additional, Krüger, Detlev H., additional, Schmidt-Chanasit, Jonas, additional, Setién, Alvaro Aguilar, additional, Cottontail, Veronika M., additional, Hemachudha, Thiravat, additional, Wacharapluesadee, Supaporn, additional, Osterrieder, Klaus, additional, Bartenschlager, Ralf, additional, Matthee, Sonja, additional, Beer, Martin, additional, Kuiken, Thijs, additional, Reusken, Chantal, additional, Leroy, Eric M., additional, Ulrich, Rainer G., additional, and Drosten, Christian, additional

Published
2013
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34. Evolutionary origins of hepatitis A virus in small mammals.

Author

Drexler, Jan Felix, Corman, Victor M., Lukashev, Alexander N., van den Brand, Judith M. A., Gmyl, Anatoly P., Brünink, Sebastian, Rasche, Andrea, Seggewiβ, Nicole, Feng, Hui, Leijten, Lonneke M., Vallo, Peter, Kuiken, Thijs, Dotzauer, Andreas, Ulrich, Rainer G., Lemon, Stanley M., and Drosten, Christian

Subjects
HEPATITIS A virus, CAPSIDS, PICORNAVIRUSES, N-terminal residues, VIRAL ecology
Abstract

Hepatitis A virus (HAV) is an ancient and ubiquitous human pathogen recovered previously only from primates. The sole species of the genus Hepatovirus, existing in both enveloped and nonenveloped forms, and with a capsid structure intermediate between that of insect viruses and mammalian picornaviruses, HAV is enigmatic in its origins. We conducted a targeted search for hepatoviruses in 15,987 specimens collected from 209 small mammal species globally and discovered highly diversified viruses in bats, rodents, hedgehogs, and shrews, which by pairwise sequence distance comprise 13 novel Hepatovirus species. Near-complete genomes from nine of these species show conservation of unique hepatovirus features, including predicted internal ribosome entry site structure, a truncated VP4 capsid protein lacking N-terminal myristoylation, a carboxyl-terminal pX extension of VP1, VP2 late domains involved in membrane envelopment, and a cis-acting replication element within the 3Dpol sequence. Antibodies in some bat sera immunoprecipitated and neutralized human HAV, suggesting conservation of critical antigenic determinants. Limited phylogenetic cosegregation among hepatoviruses and their hosts and recombination patterns are indicative of major hepatovirus host shifts in the past. Ancestral state reconstructions suggest a Hepatovirus origin in small insectivorous mammals and a rodent origin of human HAV. Patterns of infection in small mammals mimicked those of human HAV in hepatotropism, fecal shedding, acute nature, and extinction of the virus in a closed host population. The evolutionary conservation of hepatovirus structure and pathogenesis provide novel insight into the origins of HAV and highlight the utility of analyzing animal reservoirs for risk assessment of emerging viruses. [ABSTRACT FROM AUTHOR]

Published
2015
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44. Complexes assembled from TMV-derived spherical particles and entire virions of heterogeneous nature

Author

Trifonova, Ekaterina, Nikitin, Nikolai, Gmyl, Anatoly, Lazareva, Ekaterina, Karpova, Olga, and Atabekov, Joseph

Abstract

Previously, we described some structural features of spherical particles (SPs) generated by thermal remodelling of the tobacco mosaic virus. The SPs represent a universal platform that could bind various proteins. Here, we report that entire isometric virions of heterogeneous nature bind non-specifically to the SPs. Formaldehyde (FA) was used for covalent binding of a virus to the SPs surface for stabilizing the SP—virus complexes. Transmission and high resolution scanning electron microscopy showed that the SPs surface was covered with virus particles. The architecture of SP–virion complexes was examined by immunologic methods. Mean diameters of SPs and SP–human enterovirus C and SP–cauliflower mosaic virus (CaMV) compositions were determined by nanoparticle tracking analysis (NTA) in liquid. Significantly, neither free SPs nor individual virions were detected by NTA in either FA-crosslinked or FA-untreated compositions. Entirely, all virions were bound to the SPs surface and the SP sites within the SP–CaMV complexes were inaccessible for anti-SP antibodies. Likewise, the SPs immunogenicity within the FA-treated SPs–CaMV compositions was negligible. Apparently, the SP antigenic sites were hidden and masked by virions within the compositions. Previously, we reported that the SPs exhibited adjuvant activity when foreign proteins/epitopes were mixed with or crosslinked to SPs. We found that immunogenicity of entire CaMV crosslinked to SP was rather low which could be due to the above-mentioned masking of the SPs booster. Contrastingly, immunogenicity of the FA-untreated compositions increased significantly, presumably, due to partial release of virions and unmasking of some SPs-buster sites after animals immunization.

Published
2014
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45. Suppression of Injuries Caused by a Lytic RNA Virus (Mengovirus) and Their Uncoupling from Viral Reproduction by Mutual Cell/Virus Disarmament.

Author

Mikitas, Olga V., Ivin, Yuri Y., Golyshev, Sergey A., Povarova, Natalia V., Galkina, Svetlana I., Pletjushkina, Olga Y., Nadezhdina, Elena S., Gmyl, Anatoly P., and Agol, Vadim I.

Subjects
*RNA viruses, *VIRAL replication, *ELICITINS, *VIRAL proteins, *MICROBIAL growth, *CELL membranes, *APOPTOSIS, *LYTIC cycle
Abstract

viruses often elicit cell injury (cytopathic effect [CPE]), a major cause of viral diseases. CPE is usually considered to be a prerequisite for and/or consequence of efficient viral growth. Recently, we proposed that viral CPE may largely be due to host defensive and viral antidefensive activities. This study aimed to check the validity of this proposal by using as a model HeLa cells infected with mengovirus (MV). As we showed previously, infection of these cells with wild-type MV resulted in necrosis, whereas a mutant with incapacitated antidefensive ('security') viral leader (L) protein induced apoptosis. Here, we showed that several major morphological and biochemical signs of CPE (e.g., alterations in cellular and nuclear shape, plasma membrane, cytoskeleton, chromatin, and metabolic activity) in cells infected with L(-) mutants in the presence of an apoptosis inhibitor were strongly suppressed or delayed for long after completion of viral reproduction. These facts demonstrate that the efficient reproduction of a lytic virus may not directly require development of at least some pathological alterations normally accompanying infection. They also imply that L protein is involved in the control of many apparently unrelated functions. The results also suggest that the virus-activated program with competing necrotic and apoptotic branches is host encoded, with the choice between apoptosis and necrosis depending on a variety of intrinsic and extrinsic conditions. Implementation of this defensive suicidal program could be uncoupled from the viral reproduction. The possibility of such uncoupling has significant implications for the pathogenesis and treatment of viral diseases. [ABSTRACT FROM AUTHOR]

Published
2012
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46. Genomic features and evolutionary constraints in Saffold-like cardioviruses.

Author

Drexler JF, Baumgarte S, Luna LK, Stöcker A, Almeida PS, Ribeiro TC, Petersen N, Herzog P, Pedroso C, Brites C, Ribeiro Hda C Jr, Gmyl A, Drosten C, and Lukashev A

Subjects
Animals, Brazil, Cardiovirus classification, Cardiovirus isolation & purification, Cardiovirus Infections virology, Germany, Humans, Mice, Molecular Sequence Data, Nucleic Acid Conformation, Recombination, Genetic, Theilovirus genetics, Viral Nonstructural Proteins genetics, Viral Structural Proteins genetics, Cardiovirus genetics, Evolution, Molecular, Genome, Viral, RNA, Viral genetics, Sequence Analysis, DNA
Abstract

This study identified the complete genomic sequence of four type 2 and type 3 human Saffold-like cardioviruses (SLCVs) isolated in Germany and Brazil. The secondary structures of the SLCV internal ribosome entry sites (IRESs) were deduced based on RNA base-pairing conservation and co-variation, using an established Theiler's murine encephalomyelitis virus (TMEV) IRES structure as a reference. The SLCV IRES was highly similar to that of TMEV, but motifs critical in TMEV for binding of the polypyrimidine tract-binding protein (PTB) were disrupted. In TMEV, corresponding alterations have been associated with reduced neurovirulence in mice. In the non-structural genome region, there was evidence of multiple intertypic recombination events between different SLCV types. Between viruses of the same type, recombination also occurred in the capsid-encoding genome region. There were apparently no recombination events between mouse TMEV and human SLCV. In another genus of the family Picornaviridae, Enterovirus, natural recombination occurs strictly within species and can serve as an additional criterion for delimiting species. Accordingly, the results of this study suggest that SLCV and TMEV may represent distinct species within the genus Cardiovirus.

Published
2010
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