1. Protocol for the hyperinsulinemic euglycemic clamp to measure glucose kinetics in rats.
- Author
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Pereira S, Hahn MK, Humber B, Chaudhry T, Wu S, Agarwal SM, Dimitrova N, and Giacca A
- Subjects
- Animals, Rats, Glucose metabolism, Blood Glucose metabolism, Blood Glucose analysis, Insulin metabolism, Kinetics, Male, Glucose Clamp Technique methods, Insulin Resistance physiology
- Abstract
In rats, cannulation of the jugular vein and the carotid artery precedes the use of the hyperinsulinemic euglycemic clamp to determine insulin sensitivity in vivo. Here, we present a vascular surgery protocol to allow the infusion of substances via the vein and the collection of blood samples from the artery on the day of the hyperinsulinemic euglycemic clamp. We describe steps for preparing for and performing catheterization surgery. We then detail procedures for clamp preparation and its use. For complete details on the use and execution of this protocol, please refer to Pereira et al.
1 , 2 , 3 ., Competing Interests: Declaration of interests M.K.H. received consultant fees from Alkermes, Inc. S.M.A. has served as a consultant for HLS Therapeutics and Boehringer Ingelheim Canada., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
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