1. SUN proteins belong to a novel family of β-(1,3)-glucan-modifying enzymes involved in fungal morphogenesis
- Author
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Christine Schmitt, Christophe d'Enfert, Audrey Nesseir, Anne Beauvais, Patrick England, Vishukumar Aimanianda, Rémi Beau, Arnaud Firon, Marie-Christine Prévost, Amandine Gastebois, Sophie Bachellier-Bassi, Jean-Paul Latgé, Isabelle Mouyna, Aspergillus, Institut Pasteur [Paris], Biologie et Pathogénicité fongiques, Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Microscopie ultrastructurale (plate-forme), Biochimie et Biophysique des Macromolécules (Plate-forme), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was supported by grants from the European Commission (Galar Fungail 2, MRTN-CT-2003-504148, FINSysB, PITN-GA-2008-214004) (to C. d. E.) and the European Science Foundation (ESF) Fuminomics, Partenariat Sanofi-aventis-Aviesan (ITMO Maladies Infectieuses, Sanofi Aventis Research & Development TSU ID (SAR&D) TSU ID/Sanofi-Pasteur), and Agence Nationale de Recherches (ANR) (Grant 09-BLAN-0287) (to J. P. L.), ANR-09-BLAN-0287,REMODELE,Nouvelles voies métaboliques essentielles pour la biosynthèse de la paroi des champignons(2009), European Project: 214004,PEOPLE,FP7-PEOPLE-2007-1-1-ITN,FINSYSB(2008), Institut Pasteur [Paris] (IP), Biologie et Pathogénicité fongiques (BPF), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris], Biophysique des macromolécules et de leurs interactions, ANR Grant 09-BLAN-0287,ANR Grant 09-BLAN-0287,Grant 09-BLAN-0287, Institut National de la Recherche Agronomique (INRA) - Institut Pasteur [Paris], Microscopie ultrastructurale - Ultrapole (CITECH), Biophysique des macromolécules et leurs interactions, and Institut Pasteur [Paris] - Centre National de la Recherche Scientifique (CNRS)
- Subjects
Hyphal growth ,MESH : Spores, Fungal ,MESH : Molecular Sequence Data ,Glycosylation ,MESH: Sequence Homology, Amino Acid ,Conidiation ,Gene Expression ,Oligosaccharides ,Glycobiology and Extracellular Matrices ,MESH: Amino Acid Sequence ,Biochemistry ,Aspergillus fumigatus ,Cell Wall ,Gene Expression Regulation, Fungal ,Candida albicans ,Morphogenesis ,MESH : Fungal Proteins ,skin and connective tissue diseases ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,0303 health sciences ,integumentary system ,MESH : Amino Acid Sequence ,Hydrolysis ,MESH : Glycosylation ,Glucan Hydrolase Activity ,SUN ,MESH : Protein Binding ,MESH : Glycoside Hydrolases ,Spores, Fungal ,MESH: Glycosylation ,MESH : Sequence Homology, Amino Acid ,Cell biology ,[SDV] Life Sciences [q-bio] ,Aspergillus ,CELL-WALL BIOGENESIS ,MOTOR-LIKE DOMAIN ,ASPERGILLUS-FUMIGATUS ,CANDIDA-ALBICANS ,SCHIZOSACCHAROMYCES-POMBE ,SACCHAROMYCES-CEREVISIAE ,BIOFILM FORMATION ,BETA-GLUCOSIDASE ,CHITIN SYNTHASES ,GENE ,MESH: Fungal Proteins ,MESH: Aspergillus fumigatus ,MESH: Gene Expression Regulation, Fungal ,MESH: Hydrolysis ,Protein Binding ,CAZy ,MESH: Gene Expression ,Hypha ,MESH : Candida albicans ,Glycoside Hydrolases ,Molecular Sequence Data ,Hyphae ,MESH: Glycoproteins ,Biology ,Microbiology ,Cell wall ,Fungal Proteins ,03 medical and health sciences ,MESH : Hydrolysis ,MESH: Hyphae ,MESH: Spores, Fungal ,MESH : Gene Expression Regulation, Fungal ,MESH: Glycoside Hydrolases ,MESH: Protein Binding ,Amino Acid Sequence ,Molecular Biology ,030304 developmental biology ,MESH : Protein Processing, Post-Translational ,Glycoproteins ,MESH: Molecular Sequence Data ,Sequence Homology, Amino Acid ,030306 microbiology ,MESH: Candida albicans ,MESH : Oligosaccharides ,Cell Biology ,biology.organism_classification ,MESH : Glycoproteins ,[SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,Yeast ,MESH: Morphogenesis ,MESH : Gene Expression ,MESH : Morphogenesis ,MESH : Aspergillus fumigatus ,MESH: Protein Processing, Post-Translational ,MESH : Hyphae ,Carbohydrate-binding Protein ,Protein Processing, Post-Translational ,MESH: Oligosaccharides ,Biogenesis - Abstract
International audience; BACKGROUND:SUN proteins are involved in yeast morphogenesis, but their function is unknown.RESULTS:SUN protein plays a role in the Aspergillus fumigatus morphogenesis. Biochemical properties of recombinant SUN proteins were elucidated.CONCLUSION:Both Candida albicans and Aspergillus fumigatus sun proteins show a β-(1,3)-glucanase activity.SIGNIFICANCE:The mode of action of SUN proteins on β-(1,3)-glucan is unique, new, and original. In yeasts, the family of SUN proteins has been involved in cell wall biogenesis. Here, we report the characterization of SUN proteins in a filamentous fungus, Aspergillus fumigatus. The function of the two A. fumigatus SUN genes was investigated by combining reverse genetics and biochemistry. During conidial swelling and mycelial growth, the expression of AfSUN1 was strongly induced, whereas the expression of AfSUN2 was not detectable. Deletion of AfSUN1 negatively affected hyphal growth and conidiation. A closer examination of the morphological defects revealed swollen hyphae, leaky tips, intrahyphal growth, and double cell wall, suggesting that, like in yeast, AfSun1p is associated with cell wall biogenesis. In contrast to AfSUN1, deletion of AfSUN2 either in the parental strain or in the AfSUN1 single mutant strain did not affect colony and hyphal morphology. Biochemical characterization of the recombinant AfSun1p and Candida albicans Sun41p showed that both proteins had a unique hydrolysis pattern: acting on β-(1,3)-oligomers from dimer up to insoluble β-(1,3)-glucan. Referring to the CAZy database, it is clear that fungal SUN proteins represent a new family of glucan hydrolases (GH132) and play an important morphogenetic role in fungal cell wall biogenesis and septation.
- Published
- 2013