41 results on '"Glesner J"'
Search Results
2. IgE Antibody Binding Sites On Mite Group I Allergens Defined By X-ray Crystallography And Site-directed Mutagenesis: 1028
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Pomés, A., Glesner, J., Vailes, L. D., Minor, W., Chruszcz, M., and Chapman, M. D.
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- 2011
3. Crystal structure of complex between recombinant Der p 2.0103 and Fab fragment of 7A1
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Kapingidza, A.B., primary, Offermann, L.R., additional, Glesner, J., additional, Wunschmann, S., additional, Vailes, L.D., additional, Chapman, M.D.C., additional, Pomes, A., additional, and Chruszcz, M., additional
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- 2019
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4. THE CRYSTAL STRUCTURE OF DER P 1 ALLERGEN COMPLEXED WITH FAB FRAGMENT OF MAB 10B9
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Osinski, T., primary, Majorek, K.A., additional, Pomes, A., additional, Offermann, L.R., additional, Osinski, S., additional, Glesner, J., additional, Vailes, L.D., additional, Chapman, M.D., additional, Minor, W., additional, and Chruszcz, M., additional
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- 2017
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5. THE CRYSTAL STRUCTURE OF DER P 1 ALLERGEN COMPLEXED WITH FAB FRAGMENT OF MAB 5H8
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Osinski, T., primary, Majorek, K.A., additional, Pomes, A., additional, Offermann, L.R., additional, Osinski, S., additional, Glesner, J., additional, Vailes, L.D., additional, Chapman, M.D., additional, Minor, W., additional, and Chruszcz, M., additional
- Published
- 2017
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6. Crystal Structure of the dust mite allergen Der p 23 from Dermatophagoides pteronyssinus
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Pedersen, L.C., primary, Mueller, G.A., additional, Randall, T.A., additional, Glesner, J., additional, Perera, L., additional, Edwards, L.L., additional, Chapman, M.D., additional, London, R.E., additional, and Pomes, A., additional
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- 2015
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7. The crystal structure of Der p 1 allergen complexed with Fab fragment of mAb 5H8
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Osinski, T., primary, Majorek, K.A., additional, Pomes, A., additional, Offermann, L.R., additional, Osinski, S., additional, Glesner, J., additional, Vailes, L.D., additional, Chapman, M.D., additional, Minor, W., additional, and Chruszcz, M., additional
- Published
- 2015
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8. Fab fragment of Der p 1 specific antibody 10B9
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Osinski, T., primary, Majorek, K.A., additional, Pomes, A., additional, Offermann, L.R., additional, Osinski, S., additional, Glesner, J., additional, Vailes, L.D., additional, Chapman, M.D., additional, Minor, W., additional, and Chruszcz, M., additional
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- 2015
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9. The crystal structure of Der p 1 allergen complexed with Fab fragment of mAb 10B9
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Osinski, T., primary, Majorek, K.A., additional, Pomes, A., additional, Offermann, L.R., additional, Osinski, S., additional, Glesner, J., additional, Vailes, L.D., additional, Chapman, M.D., additional, Minor, W., additional, and Chruszcz, M., additional
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- 2015
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10. anti-Bla g 1 scFv
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Mueller, G.A., primary, Ankney, J.A., additional, Glesner, J., additional, Khurana, T., additional, Edwards, L.L., additional, Pedersen, L.C., additional, Perera, L., additional, Slater, J.E., additional, Pomes, A., additional, and London, R.E., additional
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- 2014
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11. Structure of Cockroach Allergen Bla g 1 Tandem Repeat as a EGFP fusion
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Mueller, G.A., primary, Pedersen, L.C., additional, Lih, F.B., additional, Glesner, J., additional, Moon, A.F., additional, Chapman, M.D., additional, Tomer, K., additional, and London, R.E., additional
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- 2013
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12. Monoclonal Antibodies for Defining Conformational Epitopes in Ara h 2 and Ara h 6
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Glesner, J., primary, Wuenschmann, S., additional, Koid, A., additional, Mueller, G.A., additional, Pedersen, L.C., additional, Chapman, M.D., additional, and Pomes, A., additional
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- 2012
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13. Mutants of the Major Cockroach Allergen, Bla g 2, Modulate T Cell Responses in Cockroach-Allergic Subjects
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Wright, P.W., primary, Glesner, J., additional, Chapman, M., additional, Pomes, A., additional, and Woodfolk, J., additional
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- 2012
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14. crystal structure of bla g 2 complexed with Fab 4C3
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Li, M., primary, Gustchina, A., additional, Glesner, J., additional, Wunschmann, S., additional, Pomes, A., additional, and Wlodawer, A., additional
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- 2010
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15. Crystal Structure of Dust Mite Allergen Der p 5
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Mueller, G.A., primary, Gosavi, R.A., additional, Krahn, J.M., additional, Edwards, L.L., additional, Cuneo, M.J., additional, Glesner, J., additional, Pomes, A., additional, Chapman, M.D., additional, London, R.E., additional, and Pedersen, L.C., additional
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- 2010
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16. American Cockroach Allergen rPer a 3 Expressed in Pichia pastoris
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Glesner, J., primary, Arruda, L.K., additional, Chapman, M.D., additional, and Pomés, A., additional
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- 2010
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17. The X-Ray Crystal Structure of Two Complexes of the Cockroach Allergen Bla g 2 with Fragments of Monoclonal Antibodies Defines Two Non-overlapping Epitopes
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Pomés, A., primary, Li, M., additional, Glesner, J., additional, Wünschmann, S., additional, King, E.M., additional, Chapman, M.D., additional, Wlodawer, A., additional, and Gustchina, A., additional
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- 2009
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18. Recombinant Der p 7 Constitutively Expressed in a Eukaryotic System Shows the Same Antigenic Properties as the Natural Allergen
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Glesner, J., primary, Chapman, M.D., additional, and Pomés, A., additional
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- 2009
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19. A pediatric randomized, controlled trial of German cockroach subcutaneous immunotherapy.
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Zoratti E, Wood R, Pomés A, Da Silva Antunes R, Altman MC, Benson B, Wheatley LM, Cho K, Calatroni A, Little FF, Pongracic J, Makhija M, Khurana Hershey GK, Sherenian MG, Rivera-Spoljaric K, Stokes JR, Gill MA, Gruchalla RS, Chambliss J, Liu AH, Kattan M, Busse PJ, Bacharier LB, Sheehan W, Kim H, Glesner J, Gergen PJ, Togias A, Baucom JL, Visness CM, Sette A, Busse WW, and Jackson DJ
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- Humans, Animals, Child, Female, Male, Adolescent, Double-Blind Method, Blattellidae immunology, Injections, Subcutaneous, Skin Tests, Desensitization, Immunologic methods, Allergens immunology, Allergens administration & dosage, Asthma immunology, Asthma therapy, Immunoglobulin E blood, Immunoglobulin E immunology
- Abstract
Background: Cockroach allergy contributes to morbidity among urban children with asthma. Few trials address the effect of subcutaneous immunotherapy (SCIT) with cockroach allergen among these at-risk children., Objectives: We sought to determine whether nasal allergen challenge (NAC) responses to cockroach allergen would improve following 1 year of SCIT., Methods: Urban children with asthma, who were cockroach-sensitized and reactive on NAC, participated in a year-long randomized double-blind placebo-controlled SCIT trial using German cockroach extract. The primary endpoint was the change in mean Total Nasal Symptom Score (TNSS) during NAC after 12 months of SCIT. Changes in nasal transcriptomic responses during NAC, skin prick test wheal size, serum allergen-specific antibody production, and T-cell responses to cockroach allergen were assessed., Results: Changes in mean NAC TNSS did not differ between SCIT-assigned (n = 28) versus placebo-assigned (n = 29) participants (P = .63). Nasal transcriptomic responses correlated with TNSS, but a treatment effect was not observed. Cockroach serum-specific IgE decreased to a similar extent in both groups, while decreased cockroach skin prick test wheal size was greater among SCIT participants (P = .04). A 200-fold increase in cockroach serum-specific IgG
4 was observed among subjects receiving SCIT (P < .001) but was unchanged in the placebo group. T-cell IL-4 responses following cockroach allergen stimulation decreased to a greater extent among SCIT versus placebo (P = .002), while no effect was observed for IL-10 or IFN-γ., Conclusions: A year of SCIT failed to alter NAC TNSS and nasal transcriptome responses to cockroach allergen challenge despite systemic effects on allergen-specific skin tests, induction of serum-specific IgG4 serum production and down-modulation of allergen-stimulated T-cell responses., (Copyright © 2024 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
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20. Structural analysis of human IgE monoclonal antibody epitopes on dust mite allergen Der p 2.
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Ball A, Khatri K, Glesner J, Vailes LD, Wünschmann S, Gabel SA, Mueller GA, Zhang J, Peebles RS Jr, Chapman MD, Smith SA, Chruszcz M, and Pomés A
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- Humans, Animals, Mice, Epitope Mapping, Crystallography, X-Ray, Receptors, IgE immunology, Receptors, IgE chemistry, Pyroglyphidae immunology, Allergens immunology, Allergens chemistry, Antigens, Dermatophagoides immunology, Antigens, Dermatophagoides chemistry, Immunoglobulin E immunology, Arthropod Proteins immunology, Arthropod Proteins chemistry, Antibodies, Monoclonal immunology, Mice, Transgenic, Epitopes immunology
- Abstract
Background: Human IgE (hIgE) mAbs against major mite allergen Der p 2 developed using human hybridoma technology were used for IgE epitope mapping and analysis of epitopes associated with the hIgE repertoire., Objective: We sought to elucidate the new hIgE mAb 4C8 epitope on Der p 2 and compare it to the hIgE mAb 2F10 epitope in the context of the allergenic structure of Der p 2., Methods: X-ray crystallography was used to determine the epitope of anti-Der p 2 hIgE mAb 4C8. Epitope mutants created by targeted mutagenesis were analyzed by immunoassays and in vivo using a human high-affinity IgE receptor (FcεRIα)-transgenic mouse model of passive systemic anaphylaxis., Results: The structure of recombinant Der p 2 with hIgE mAb 4C8 Fab was determined at 3.05 Å. The newly identified epitope region does not overlap with the hIgE mAb 2F10 epitope or the region recognized by 3 overlapping hIgE mAbs (1B8, 5D10, and 2G1). Compared with wild-type Der p 2, single or double 4C8 and 2F10 epitope mutants bound less IgE antibodies from allergic patients by as much as 93%. Human FcεRIα-transgenic mice sensitized by hIgE mAbs, which were susceptible to anaphylaxis when challenged with wild-type Der p 2, could no longer cross-link FcεRI to induce anaphylaxis when challenged with the epitope mutants., Conclusions: These data establish the structural basis of allergenicity of 2 hIgE mAb nonoverlapping epitopes on Der p 2, which appear to make important contributions to the hIgE repertoire against Der p 2 and provide molecular targets for future design of allergy therapeutics., Competing Interests: Disclosure statement Research reported in this publication was supported by the National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) (award nos. R01AI077653-13 [to A.P., M.D.C., and M.C.] and R01AI155668 and R21AI123307 [to S.A.S.]). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. The research was supported in part by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences (Z01-ES102906 [to G.A.M.]). The structural results shown in this report are derived from data collected at Southeast Regional Collaborative Access Team (SER-CAT; 22ID) beamline at the Advanced Photon Source, Argonne National Laboratory. Supporting institutions may be found at www.ser-cat.org/members.html. Use of the Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences (contract nos. DE-AC02-06CH11357 and W-31-109-Eng-38). This work was partially supported by an ASPIRE III grant from the Office of the Vice President of Research at the University of South Carolina. This research is funded by the above mentioned NIH/NIAID award to InBio. Disclosure of potential conflict of interest: A. Pomés is an employee of InBio; and is the contact principal investigator of the NIH R01 award that provided funding for the study. M.D. Chapman is an employee of InBio; has a financial interest in InBio; and is a co-investigator on the NIH R01 award. A. Ball is an employee of InBio. The hIgE mAbs and some of the allergens described herein were produced by InBio. InBio has a license agreement with the VUMC for commercialization of hIgE mAbs for research and diagnostic purposes. The hIgE mAbs covered by this agreement are available from InBio (www.inbio.com). S.A. Smith is an inventor on US patent 10908168-B2 for generation of hIgE mAbs; has received patent royalties; and has related patents pending. The rest of the authors declare that they have no relevant conflicts of interest., (Copyright © 2024 American Academy of Allergy, Asthma & Immunology. All rights reserved.)
- Published
- 2024
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21. Structural, Biophysical, and Computational Studies of a Murine Light Chain Dimer.
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Arriaza RH, Kapingidza AB, Dolamore C, Khatri K, O'Malley A, Glesner J, Wuenschmann S, Hyduke NP, Easley W, Chhiv C, Pomés A, and Chruszcz M
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- Animals, Mice, Antibodies, Monoclonal chemistry, Antibodies, Monoclonal immunology, Models, Molecular, Protein Binding, Crystallography, X-Ray, Protein Conformation, Immunoglobulin Heavy Chains chemistry, Immunoglobulin Light Chains chemistry, Immunoglobulin Light Chains metabolism, Protein Multimerization
- Abstract
Antibodies are widely used in medicinal and scientific research due to their ability to bind to a specific antigen. Most often, antibodies are composed of heavy and light chain domains. Under physiological conditions, light chains are produced in excess, as compared to the heavy chain. It is now known that light chains are not silent partners of the heavy chain and can modulate the immune response independently. In this work, the first crystal structure of a light chain dimer originating from mice is described. It represents the light chain dimer of 6A8, a monoclonal antibody specific to the allergen Der f 1. Building on the unexpected occurrence of this kind of dimer, we have demonstrated that this light chain is stable in solution alone. Moreover, enzyme-linked immunosorbent assays (ELISA) have revealed that, when the light chain is not partnered to its corresponding heavy chain, it interacts non-specifically with a wide range of proteins. Computational studies were used to provide insight on the role of the 6A8 heavy chain domain in the specific binding to Der f 1. Overall, this work demonstrates and supports the ongoing notion that light chains can function by themselves and are not silent partners of heavy chains.
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- 2024
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22. Unique allergen-specific human IgE monoclonal antibodies derived from patients with allergic disease.
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Smith BRE, Reid Black K, Bermingham M, Agah S, Glesner J, Versteeg SA, van Ree R, Pena-Amelunxen G, Aglas L, Smith SA, Pomés A, and Chapman MD
- Abstract
Introduction: Allergic reactions are mediated by human IgE antibodies that bind to and cross-link allergen molecules. The sites on allergens that are recognized by IgE antibodies have been difficult to investigate because of the paucity of IgE antibodies in a human serum. Here, we report the production of unique human IgE monoclonal antibodies to major inhaled allergens and food allergens that can be produced at scale in perpetuity., Materials and Methods: The IgE antibodies were derived from peripheral blood mononuclear cells of symptomatic allergic patients, mostly children aged 3-18 years, using hybridoma fusion technology. Total IgE and allergen-specific IgE was measured by ImmunoCAP. Their specificity was confirmed through ELISA and immunoblotting. Allergenic potency measurements were determined by ImmunoCAP inhibition. Biological activity was determined in vitro by comparing β -hexosaminidase release from a humanized rat basophilic cell line., Results: Human IgE monoclonal antibodies ( n = 33) were derived from 17 allergic patients with symptoms of allergic rhinitis, asthma, atopic dermatitis, food allergy, eosinophilic esophagitis, or red meat allergy. The antibodies were specific for five inhaled allergens, nine food allergens, and alpha-gal and had high levels of IgE (53,450-1,702,500 kU/L) with ratios of specific IgE to total IgE ranging from <0.01 to 1.39. Sigmoidal allergen binding curves were obtained through ELISA, with low limits of detection (<1 kU/L). Allergen specificity was confirmed through immunoblotting. Pairs of IgE monoclonal antibodies to Ara h 6 were identified that cross-linked after allergen stimulation and induced release of significant levels of β -hexosaminidase (35%-80%) from a humanized rat basophilic cell line., Conclusions: Human IgE monoclonal antibodies are unique antibody molecules with potential applications in allergy diagnosis, allergen standardization, and identification of allergenic epitopes for the development of allergy therapeutics. The IgE antibody probes will enable the unequivocal localization and validation of allergenic epitopes., Competing Interests: The research approach was developed by several authors who are employees of InBio (BS, KR, MB, SA, JG, AP, and MC). The hIgE mAb and some allergens used in this study were produced by InBio. AP is a contact principal investigator of the NIH R01 award that provided funding for the study. MC has a financial interest in InBio and is a co-investigator for the NIH R01 award. InBio has a license agreement with Vanderbilt University Medical Center for the commercialization of the hIgE mAb for research and diagnostic purposes. The hIgE mAb covered by this agreement are available from InBio (www.inbio.com). SS is an inventor on US patent 10908168-B2 for the generation of human IgE monoclonal antibodies, has received patent royalties, and has related patents pending. RR is a consultant for HAL Allergy BV, Citeq BV, Angany Inc., Reacta Healthcare Ltd., Mission MightyMe, and AB Enzymes; receives speaker fees from HAL Allergy BV, ALK, and Thermo Fisher Scientific; and has stock options with Angany Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (© 2023 Smith, Reid Black, Bermingham, Agah, Glesner, Versteeg, van Ree, Pena-Amelunxen, Aglas, Smith, Pomés and Chapman.)
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- 2023
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23. Human IgE monoclonal antibody recognition of mite allergen Der p 2 defines structural basis of an epitope for IgE cross-linking and anaphylaxis in vivo .
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Khatri K, Richardson CM, Glesner J, Kapingidza AB, Mueller GA, Zhang J, Dolamore C, Vailes LD, Wünschmann S, Peebles RS Jr, Chapman MD, Smith SA, Chruszcz M, and Pomés A
- Abstract
Immunoglobulin E (IgE) antibody is a critical effector molecule for adaptive allergen-induced immune responses, which affect up to 40% of the population worldwide. Allergens are usually innocuous molecules but induce IgE antibody production in allergic subjects. Allergen cross-linking of IgE bound to its high affinity receptor (FcεRI) on mast cells and basophils triggers release of histamine and other mediators that cause allergic symptoms. Little is known about the direct allergen-IgE antibody interaction due to the polyclonal nature of serum IgE and the low frequency of IgE-producing B cells in blood. Here, we report the X-ray crystal structure of a house dust mite allergen, Der p 2, in complex with Fab of a human IgE monoclonal antibody (mAb) isolated by hybridoma technology using human B cells from an allergic subject. This IgE mAb, 2F10, has the correct pairing of heavy and light chains as it occurs in vivo . Key amino acids forming the IgE epitope on Der p 2 were identified. Mutation of these residues ablated their functional ability to cross-link IgE in a mouse model of passive systemic anaphylaxis. These analyses revealed an important conformational epitope associated with the IgE antibody repertoire to a major mite allergen., (© The Author(s) 2022. Published by Oxford University Press on behalf of the National Academy of Sciences.)
- Published
- 2022
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24. Heterogeneity of magnitude, allergen immunodominance, and cytokine polarization of cockroach allergen-specific T cell responses in allergic sensitized children.
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da Silva Antunes R, Sutherland A, Frazier A, Schulten V, Pomés A, Glesner J, Calatroni A, Altman MC, Wood RA, O'Connor GT, Pongracic JA, Khurana Hershey GK, Kercsmar CM, Gruchalla RS, Gill M, Liu AH, Zoratti E, Kattan M, Busse PJ, Bacharier LB, Teach SJ, Wheatley LM, Togias A, Busse WW, Jackson DJ, and Sette A
- Abstract
Background: Characterization of allergic responses to cockroach (CR), a common aeroallergen associated with asthma, has focused mainly on IgE reactivity, but little is known about T cell responses, particularly in children. We conducted a functional evaluation of CR allergen-specific T cell reactivity in a cohort of CR allergic children with asthma., Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from 71 children, with mild-to-moderate asthma who were enrolled in a CR immunotherapy (IT) clinical trial, prior to treatment initiation. PBMC were stimulated with peptide pools derived from 11 CR allergens, and CD4+ T cell responses assessed by intracellular cytokine staining., Results: Highly heterogeneous responses in T cell reactivity were observed among participants, both in terms of the magnitude of cytokine response and allergen immunodominance. Reactivity against Bla g 9 and Bla g 5 was most frequent. The phenotype of the T cell response was dominated by IL-4 production and a Th2 polarized profile in 54.9% of participants, but IFNγ production and Th1 polarization was observed in 25.3% of the participants. The numbers of regulatory CD4+ T cells were also highly variable and the magnitude of effector responses and Th2 polarization were positively correlated with serum IgE levels specific to a clinical CR extract., Conclusions: Our results demonstrate that in children with mild-to-moderate asthma, CR-specific T cell responses display a wide range of magnitude, allergen dominance, and polarization. These results will enable examination of whether any of the variables measured are affected by IT and/or are predictive of clinical outcomes., Competing Interests: All authors declare no conflict of interest., (© 2021 The Authors. Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology.)
- Published
- 2021
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25. IgE and T Cell Reactivity to a Comprehensive Panel of Cockroach Allergens in Relation to Disease.
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Pomés A, Schulten V, Glesner J, da Silva Antunes R, Sutherland A, Bacharier LB, Beigelman A, Busse P, Frazier A, and Sette A
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- Adult, Animals, Female, Humans, Male, Middle Aged, Allergens immunology, Blattellidae, Hypersensitivity immunology, Immunoglobulin E immunology, Insect Proteins immunology, T-Lymphocytes immunology
- Abstract
IgE sensitization to cockroach allergens is associated with development of allergic diseases, such as asthma. To understand the relevance of different cockroach allergens for diagnosis and immunotherapy, a comprehensive analysis of IgE antibody levels and T cell reactivity to an expanded set of cockroach allergens and their relationship to disease was performed in a cohort of USA cockroach sensitized patients. IgE antibody levels to recombinant chitinase and hemocyanin were measured for 23 subjects by custom-made ImmunoCAPs and compared with IgE levels to eight cockroach allergens we previously reported for the same cohort. Ex vivo T cell activation (Ox40/PDL-1 expression) of PBMCs stimulated with peptide pools derived from 11 German cockroach proteins, including nine official cockroach allergens, plus chitinase and vitellogenin, was determined by flow cytometry. IgE prevalences to chitinase (17%) and hemocyanin (44%) were comparable to values for the other eight allergens that we previously reported (21-57%). Hemocyanin (Bla g 3), was a major allergen (one to which more than 50% of patients with an allergy to its source react) for a sub-group of 15 highly cockroach-sensitized subjects (IgE > 3.5 kU
A /L: 53%). Chitinase was officially named as new allergen Bla g 12. Cockroach-specific IgE levels in plasma showed excellent correlation with the sum of 10 allergen-specific IgE (r = 0.94, p < 0.001). T cell reactivity to 11 proteins was highly variable among subjects, the highest being for vitellogenin, followed by Bla g 3. The main finding was that cockroach allergen-specific IgE and T cell reactivity patterns were unique per subject, and lacked immunodominant allergens and correlation with clinical phenotype/disease severity in the studied cohort. Knowing the subject-specific B/T cell reactivity profiles to a comprehensive panel of cockroach allergens will contribute to diagnosis of cockroach allergy and will be important for planning and assessing allergen immunotherapy outcomes, according to the allergen content in therapeutic cockroach extracts., Competing Interests: AP is an employee of Indoor Biotechnologies, Inc. and the contact principal investigator of the NIH R01 Award that funded the study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Pomés, Schulten, Glesner, da Silva Antunes, Sutherland, Bacharier, Beigelman, Busse, Frazier and Sette.)- Published
- 2021
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26. Mapping Human Monoclonal IgE Epitopes on the Major Dust Mite Allergen Der p 2.
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Mueller GA, Glesner J, Daniel JL, Zhang J, Hyduke N, Richardson CM, DeRose EF, Chapman MD, Peebles RS Jr, A Smith S, and Pomés A
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- Humans, Antibodies, Monoclonal immunology, Antigens, Dermatophagoides immunology, Arthropod Proteins immunology, Epitope Mapping, Epitopes immunology, Immunoglobulin E immunology
- Abstract
IgE Abs drive the symptoms of allergic disease upon cross-linking allergens on mast cells or basophils. If the IgE binding sites on the allergens could be identified, it may be useful for creating new forms of immunotherapy. However, direct knowledge of the human IgE (hIgE) epitopes is limited because of the very low frequency of IgE-producing B cells in blood. A new hybridoma technology using human B cells from house dust mite-allergic patients was used to identify four Der p 2-specific hIgE mAbs. Their relative binding sites were assessed and compared by immunoassays with three previously studied murine IgG mAbs. Immunoassays showed that the recognition of Der p 2 by the first three hIgE was inhibited by a single murine IgG, but the fourth hIgE recognized a different epitope from all the other mAbs. The functional ability of the hIgE that bind different epitopes to cross-link Der p 2 was demonstrated in a mouse model of passive systemic anaphylaxis. Nuclear magnetic resonance analyses of Der p 2 in complex with IgG and IgE Abs were used to identify specific residues in the epitopes. To our knowledge, the combination of immunoassays to distinguish overlapping epitopes and nuclear magnetic resonance analyses to identify specific residues involved in Ab binding provided the first epitope mapping of hIgE mAbs to an allergen. The technologies developed in this study will be useful in high-resolution mapping of human epitopes on other Ags and the design of improved therapeutics., (Copyright © 2020 by The American Association of Immunologists, Inc.)
- Published
- 2020
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27. A Human IgE Antibody Binding Site on Der p 2 for the Design of a Recombinant Allergen for Immunotherapy.
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Glesner J, Kapingidza AB, Godzwon M, Offermann LR, Mueller GA, DeRose EF, Wright P, Richardson CM, Woodfolk JA, Vailes LD, Wünschmann S, London RE, Chapman MD, Ohlin M, Chruszcz M, and Pomés A
- Subjects
- Antibodies, Monoclonal chemistry, Antigens, Dermatophagoides chemistry, Arthropod Proteins chemistry, Crystallography, X-Ray, Epitopes immunology, Humans, Magnetic Resonance Spectroscopy, Mutagenesis, Site-Directed, Protein Conformation, Recombinant Proteins immunology, Antibodies, Monoclonal immunology, Antigens, Dermatophagoides immunology, Arthropod Proteins immunology, Binding Sites, Antibody, Desensitization, Immunologic methods, Immunoglobulin E immunology
- Abstract
Der p 2 is one of the most important allergens from the house dust mite Dermatophagoides pteronyssinus Identification of human IgE Ab binding epitopes can be used for rational design of allergens with reduced IgE reactivity for therapy. Antigenic analysis of Der p 2 was performed by site-directed mutagenesis based on the x-ray crystal structure of the allergen in complex with a Fab from the murine IgG mAb 7A1 that binds an epitope overlapping with human IgE binding sites. Conformational changes upon Ab binding were confirmed by nuclear magnetic resonance using a 7A1-single-chain variable fragment. In addition, a human IgE Ab construct that interferes with mAb 7A1 binding was isolated from a combinatorial phage-display library constructed from a mite-allergic patient and expressed as two recombinant forms (single-chain Fab in Pichia pastoris and Fab in Escherichia coli ). These two IgE Ab constructs and the mAb 7A1 failed to recognize two Der p 2 epitope double mutants designed to abolish the allergen-Ab interaction while preserving the fold necessary to bind Abs at other sites of the allergen surface. A 10-100-fold reduction in binding of IgE from allergic subjects to the mutants additionally showed that the residues mutated were involved in IgE Ab binding. In summary, mutagenesis of a Der p 2 epitope defined by x-ray crystallography revealed an IgE Ab binding site that will be considered for the design of hypoallergens for immunotherapy., (Copyright © 2019 by The American Association of Immunologists, Inc.)
- Published
- 2019
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28. Cockroach allergen component analysis of children with or without asthma and rhinitis in an inner-city birth cohort.
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Pomés A, Glesner J, Calatroni A, Visness CM, Wood RA, O'Connor GT, Kattan M, Bacharier LB, Wheatley LM, Gern JE, and Busse WW
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- Animals, Asthma blood, Asthma etiology, Child, Cohort Studies, Environmental Exposure adverse effects, Female, Humans, Immunoglobulin E blood, Immunoglobulin E immunology, Male, Rhinitis blood, Rhinitis etiology, Urban Population, Allergens immunology, Asthma immunology, Cockroaches immunology, Rhinitis immunology
- Abstract
Background: Cockroach is one of the most important sources of indoor allergens and can lead to IgE sensitization and development of rhinitis and asthma., Objective: We sought to perform a cockroach allergen component analysis to determine the allergens and antibody levels and patterns of sensitization associated with asthma and rhinitis., Methods: Antibody (IgE, IgG, and IgG
4 ) levels to total cockroach and 8 cockroach allergens were determined in 2 groups of cockroach-sensitized 10-year-old children with (n = 19) or without (n = 28) asthma and rhinitis. Allergen-specific antibody levels were measured in streptavidin ImmunoCAPs loaded with each of the recombinant allergens from groups 1, 2, 4, 5, 6, 7, 9, and 11, and total cockroach-specific IgE levels were measured with the i6 ImmunoCAP., Results: IgE antibody levels to cockroach allergens and extract, but not IgG or IgG4 antibody levels, differed between subjects with and without asthma and rhinitis. Specifically, recognition of more cockroach allergens with higher allergen-specific IgE levels was associated with disease. Variable patterns of sensitization with no immunodominant allergens were found in both groups. There was a good correlation between the sum of allergen-specific IgE and total cockroach IgE levels (r = 0.86, P < .001)., Conclusions: Component analysis of 8 cockroach allergens revealed significant differences in IgE reactivity associated with the presence of asthma and rhinitis. Allergen-specific IgE titers and sensitization profiles were associated with asthma and rhinitis., (Copyright © 2019. Published by Elsevier Inc.)- Published
- 2019
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29. Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency for IgE reactivity.
- Author
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Glesner J, Filep S, Vailes LD, Wünschmann S, Chapman MD, Birrueta G, Frazier A, Jeong KY, Schal C, Bacharier L, Beigelman A, Busse P, Schulten V, Sette A, and Pomés A
- Subjects
- Animals, Female, Humans, Hypersensitivity etiology, Male, Allergens immunology, Blattellidae immunology, Hypersensitivity immunology, Immunoglobulin E immunology, Insect Proteins immunology
- Abstract
Background: Cockroach allergens are an important cause of IgE-mediated sensitization in inner-city asthmatic patients. However, cockroach extracts used for diagnosis and immunotherapy are not standardized., Objective: We sought to determine the allergen content of nonstandardized German cockroach extracts and the levels of sensitization to an expanded set of cockroach allergens as determinants of in vitro extract potency for IgE reactivity., Methods: Twelve German cockroach extracts were compared for allergen content and potency of IgE reactivity. Bla g 1, Bla g 2, and Bla g 5 were measured by using immunoassays. IgE antibody levels to 8 purified recombinant allergens from groups 1, 2, 4, 5, 6, 7, 9, and 11 were measured by using ImmunoCAP. IgE antibody binding inhibition assays were performed to assess extract in vitro potencies (concentration inhibiting 30% of the total IgE antibody-binding inhibition) relative to an arbitrarily selected reference extract in 5 patients with cockroach allergy., Results: Allergen levels were highly variable. Three new major allergens (groups 6, 9, and 11), were identified among highly cockroach-sensitized subjects (CAP class ≥ 3). Sensitization profiles were unique per subject without immunodominant allergens. The sum of IgE to 8 allergen components showed a good correlation with cockroach-specific IgE levels (r = 0.88, P < .001). In vitro potencies varied among different extracts per subject and among subjects for each extract., Conclusions: The in vitro potency of German cockroach extracts for IgE reactivity depends on allergen content and allergen-specific IgE titers of patients with cockroach allergy. These factors are relevant for selection of potent extracts to be used for immunotherapy and for the design and interpretation of data from immunotherapy trials., (Copyright © 2018 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2019
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30. Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors.
- Author
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Birrueta G, Frazier A, Pomés A, Glesner J, Filep S, Schal C, Jeong KY, McMurtrey C, Vander Schans T, Hildebrand WH, Busse P, Beigelman A, Bacharier LB, Peters B, Sette A, and Schulten V
- Subjects
- Animals, Blattellidae chemistry, Cytokines biosynthesis, Humans, Hypersensitivity diagnosis, Hypersensitivity metabolism, Immunoglobulin E immunology, Leukocytes, Mononuclear immunology, Leukocytes, Mononuclear metabolism, Skin Tests, T-Lymphocytes metabolism, Tissue Donors, Tissue Extracts chemistry, Allergens immunology, Blattellidae immunology, Hypersensitivity immunology, Lymphocyte Activation immunology, T-Lymphocytes immunology, Tissue Extracts immunology
- Abstract
German cockroach extract is used clinically to evaluate allergen-specific sensitization and for subcutaneous allergen-specific immunotherapy, though there are no guidelines for standardization in its manufacture. We performed an immunological evaluation of 12 different cockroach extracts prepared from different sources and their potency to induce allergen-specific T cell reactivity. PBMC from 13 cockroach allergic donors were expanded in vitro with 12 different German cockroach extracts. After culture expansion, cells were re-stimulated with the different extracts and T cell responses were assessed by FluoroSpot (IL-5, IFNγ and IL-10 production). In parallel to the extracts, single allergen peptide pools for allergens from groups 1, 2, 4, 5, and 11 were tested to determine allergen immunodominance. Furthermore, to assess allergy specificity, PBMC from 13 non-allergic donors were also tested with the most potent extract and T cell responses were compared to the allergic cohort. Dramatic variations in T cell reactivity were observed to the different cockroach extract batches. Response magnitudes varied over 3 logs within a single donor. IL-5 production in the allergic cohort was significantly higher compared to the non-allergic cohort (p=0.004). Allergen content determination by ELISA detected much lower concentrations of Bla g 5 compared to Bla g 1 and 2. Mass spectrometric analysis revealed that Bla g 5 was present in similar amounts to Bla g 1 and 2 in extracts made from whole body, whereas it was not detected in extracts made from fecal matter, suggesting that Bla g 5 is not excreted into feces. Different donors exhibit different response patterns to different extracts, potentially dependent on the donor-specific T cell allergen immunodominance pattern and the allergen content of the extract tested. These findings have dramatic implications for the selection of potent extracts used for diagnostic purposes or allergen-specific immunotherapy.
- Published
- 2019
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31. Antigenic Determinants of Der p 1: Specificity and Cross-Reactivity Associated with IgE Antibody Recognition.
- Author
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Glesner J, Vailes LD, Schlachter C, Mank N, Minor W, Osinski T, Chruszcz M, Chapman MD, and Pomés A
- Subjects
- Animals, Antibodies, Monoclonal immunology, Antigen-Antibody Complex chemistry, Binding Sites, Antibody, Cross Reactions, Mutagenesis, Site-Directed, Antigens, Dermatophagoides immunology, Arthropod Proteins immunology, Cysteine Endopeptidases immunology, Epitopes immunology, Immunoglobulin E immunology
- Abstract
Der p 1 and Der f 1 are major allergens from Dermatophagoides pteronyssinus and D. farinae, respectively. An analysis of antigenic determinants on both allergens was performed by site-directed mutagenesis. The analysis was based on the x-ray crystal structures of the allergens in complex with Fab fragments of three murine mAbs that interfere with IgE Ab binding: the two Der p 1-specific mAbs 5H8 and 10B9, and the cross-reactive mAb 4C1. On one hand, selected residues in the epitopes for mAb 5H8 and mAb 4C1 were substituted with amino acids that resulted in impaired Ab binding to Der p 1. On the other hand, an epitope for the Der p 1-specific mAb 10B9, which partially overlaps with mAb 4C1, was created in Der f 1. The mutation of 1-3 aa residues in Der f 1 was sufficient to bind mAb 10B9. These residues form hydrogen bonds with CDRs of the Ab other than H CDR3. This observation unveils an exception to the dominant role of H CDR3 commonly observed in Ag recognition. Overall, this study resulted in the identification of important residues for mAb and IgE Ab recognition in group 1 mite allergens. This information can be used to engineer allergen mutants with reduced IgE Ab binding for immunotherapy., (Copyright © 2017 by The American Association of Immunologists, Inc.)
- Published
- 2017
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32. Antigenic Determinants of the Bilobal Cockroach Allergen Bla g 2.
- Author
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Woodfolk JA, Glesner J, Wright PW, Kepley CL, Li M, Himly M, Muehling LM, Gustchina A, Wlodawer A, Chapman MD, and Pomés A
- Subjects
- Allergens immunology, Animals, Antibodies, Monoclonal immunology, Aspartic Acid Endopeptidases immunology, Asthma etiology, CD4-Positive T-Lymphocytes cytology, Crystallography, X-Ray, Epitopes, T-Lymphocyte chemistry, Humans, Immunoglobulin E immunology, Insect Proteins immunology, Mutagenesis, Mutation, Pichia, Protein Binding, Protein Conformation, Th1 Cells cytology, Th2 Cells cytology, Allergens chemistry, Aspartic Acid Endopeptidases chemistry, Cockroaches chemistry, Insect Proteins chemistry
- Abstract
Bla g 2 is a major indoor cockroach allergen associated with the development of asthma. Antigenic determinants on Bla g 2 were analyzed by mutagenesis based on the structure of the allergen alone and in complex with monoclonal antibodies that interfere with IgE antibody binding. The structural analysis revealed mechanisms of allergen-antibody recognition through cation-π interactions. Single and multiple Bla g 2 mutants were expressed in Pichia pastoris and purified. The triple mutant K132A/K251A/F162Y showed an ∼100-fold reduced capacity to bind IgE, while preserving the native molecular fold, as proven by x-ray crystallography. This mutant was still able to induce mast cell release. T-cell responses were assessed by analyzing Th1/Th2 cytokine production and the CD4(+) T-cell phenotype in peripheral blood mononuclear cell cultures. Although T-cell activating capacity was similar for the KKF mutant and Bla g 2 based on CD25 expression, the KKF mutant was a weaker inducer of the Th2 cytokine IL-13. Furthermore, this mutant induced IL-10 from a non-T-cell source at higher levels that those induced by Bla g 2. Our findings demonstrate that a rational design of site-directed mutagenesis was effective in producing a mutant with only 3 amino acid substitutions that maintained the same fold as wild type Bla g 2. These residues, which were involved in IgE antibody binding, endowed Bla g 2 with a T-cell modulatory capacity. The antigenic analysis of Bla g 2 will be useful for the subsequent development of recombinant allergen vaccines., (© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Published
- 2016
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33. Analysis of glutathione S-transferase allergen cross-reactivity in a North American population: Relevance for molecular diagnosis.
- Author
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Mueller GA, Pedersen LC, Glesner J, Edwards LL, Zakzuk J, London RE, Arruda LK, Chapman MD, Caraballo L, and Pomés A
- Subjects
- Animals, Cockroaches, Cross Reactions, Crystallization, Helminths, Humans, Immunoglobulin E blood, Mice, Molecular Mimicry, North America, Pathology, Molecular, Pyroglyphidae, Tropical Climate, Allergens immunology, Antigens, Dermatophagoides immunology, Antigens, Helminth immunology, Arthropod Proteins immunology, Glutathione Transferase immunology, Insect Proteins immunology, Population Groups
- Abstract
Background: It is not clear whether cross-reactivity or cosensitization to glutathione S-transferases (GSTs) occurs in tropical and subtropical environments. In the United States, Bla g 5 is the most important GST allergen and lack of coexposure to GSTs from certain species allows a better assessment of cross-reactivity., Objectives: To examine the molecular structure of GST allergens from cockroach (Bla g 5), dust mites (Der p 8 and Blo t 8), and helminth (Asc s 13) for potential cross-reactive sites, and to assess the IgE cross-reactivity of sensitized patients from a temperate climate for these allergens for molecular diagnostic purposes., Methods: Four crystal structures were determined. Sera from patients allergic to cockroach and mite were tested for IgE reactivity to these GSTs. A panel of 6 murine anti-Bla g 5 mAb was assessed for cross-reactivity with the other 3 GSTs using antibody binding assays., Results: Comparisons of the allergen structures, formed by 2-domain monomers that dimerize, revealed few contiguous regions of similar exposed residues, rendering cross-reactivity unlikely. Accordingly, anti-Bla g 5 or anti-Der p 8 IgE from North American patients did not recognize Der p 8 or Bla g 5, respectively, and neither showed binding to Blo t 8 or Asc s 13. A weaker binding of anti-Bla g 5 IgE to Der p 8 versus Bla g 5 (∼ 100-fold) was observed by inhibition assays, similar to a weak recognition of Der p 8 by anti-Bla g 5 mAb. Patients from tropical Colombia had IgE to all 4 GSTs., Conclusions: The lack of significant IgE cross-reactivity among the 4 GSTs is in agreement with the low shared amino acid identity at the molecular surface. Each GST is needed for accurate molecular diagnosis in different geographic areas., (Copyright © 2015 American Academy of Allergy, Asthma & Immunology. All rights reserved.)
- Published
- 2015
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34. Structural Analysis of Der p 1-Antibody Complexes and Comparison with Complexes of Proteins or Peptides with Monoclonal Antibodies.
- Author
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Osinski T, Pomés A, Majorek KA, Glesner J, Offermann LR, Vailes LD, Chapman MD, Minor W, and Chruszcz M
- Subjects
- Amino Acid Sequence, Animals, Antibodies, Monoclonal immunology, Antigen-Antibody Complex immunology, Antigens, Dermatophagoides immunology, Antigens, Dermatophagoides isolation & purification, Arthropod Proteins immunology, Arthropod Proteins isolation & purification, Binding Sites, Crystallography, X-Ray, Cysteine Endopeptidases immunology, Cysteine Endopeptidases isolation & purification, Epitopes chemistry, Epitopes immunology, Hydrogen Bonding, Immunoglobulin Fab Fragments immunology, Models, Molecular, Molecular Sequence Data, Peptides immunology, Protein Binding, Protein Structure, Secondary, Pyroglyphidae chemistry, Pyroglyphidae immunology, Sequence Alignment, Antibodies, Monoclonal chemistry, Antigen-Antibody Complex chemistry, Antigens, Dermatophagoides chemistry, Arthropod Proteins chemistry, Cysteine Endopeptidases chemistry, Immunoglobulin Fab Fragments chemistry, Peptides chemistry
- Abstract
Der p 1 is a major allergen from the house dust mite, Dermatophagoides pteronyssinus, that belongs to the papain-like cysteine protease family. To investigate the antigenic determinants of Der p 1, we determined two crystal structures of Der p 1 in complex with the Fab fragments of mAbs 5H8 or 10B9. Epitopes for these two Der p 1-specific Abs are located in different, nonoverlapping parts of the Der p 1 molecule. Nevertheless, surface area and identity of the amino acid residues involved in hydrogen bonds between allergen and Ab are similar. The epitope for mAb 10B9 only showed a partial overlap with the previously reported epitope for mAb 4C1, a cross-reactive mAb that binds Der p 1 and its homolog Der f 1 from Dermatophagoides farinae. Upon binding to Der p 1, the Fab fragment of mAb 10B9 was found to form a very rare α helix in its third CDR of the H chain. To provide an overview of the surface properties of the interfaces formed by the complexes of Der p 1-10B9 and Der p 1-5H8, along with the complexes of 4C1 with Der p 1 and Der f 1, a broad analysis of the surfaces and hydrogen bonds of all complexes of Fab-protein or Fab-peptide was performed. This work provides detailed insight into the cross-reactive and specific allergen-Ab interactions in group 1 mite allergens. The surface data of Fab-protein and Fab-peptide interfaces can be used in the design of conformational epitopes with reduced Ab binding for immunotherapy., (Copyright © 2015 by The American Association of Immunologists, Inc.)
- Published
- 2015
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35. Characterization of an anti-Bla g 1 scFv: epitope mapping and cross-reactivity.
- Author
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Mueller GA, Ankney JA, Glesner J, Khurana T, Edwards LL, Pedersen LC, Perera L, Slater JE, Pomés A, and London RE
- Subjects
- Allergens genetics, Animals, Binding Sites, Antibody immunology, Cross Reactions immunology, Crystallography, X-Ray, Epitope Mapping, Epitopes immunology, Epitopes ultrastructure, Humans, Immunoglobulin E immunology, Models, Molecular, Mutation, Allergens immunology, Cockroaches immunology, Single-Chain Antibodies immunology, Single-Chain Antibodies ultrastructure
- Abstract
Bla g 1 is a major allergen from Blatella germanica and one of the primary allergens used to assess cockroach allergen exposure. The epitope of an anti-Bla g 1 scFv was mapped in order to better understand cross reactivity with other group 1 cockroach allergens and patient IgE epitopes. X-ray crystallography was used to determine the structure of the scFv. The scFv epitope on Bla g 1 was located by alanine scanning site-directed mutagenesis and ELISA. Twenty-six rBla g 1-GST alanine mutants were evaluated for variations in binding to the scFv compared to the wild type allergen. Six mutants showed a significant difference in scFv binding affinity. These mutations clustered to form a discontinuous epitope mainly comprising two helices of Bla g 1. The allergen-scFv complex was modeled based on the results, and the epitope region was found to have low sequence similarity with Per a 1, especially among the residues identified as functionally important for the scFv binding to Bla g 1. Indeed, the scFv failed to bind Per a 1 in American cockroach extract. The scFv was unable to inhibit the binding of IgE antibodies from a highly cockroach allergic patient to Bla g 1. Based on the surface area of Bla g 1 occluded by the scFv, putative regions of patient IgE-Bla g 1 interactions can be inferred. This scFv could be best utilized as a capture antibody in an IgE detection ELISA, or to differentiate Bla g 1 from Per a 1 in environmental exposure assays., (Published by Elsevier Ltd.)
- Published
- 2014
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36. The novel structure of the cockroach allergen Bla g 1 has implications for allergenicity and exposure assessment.
- Author
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Mueller GA, Pedersen LC, Lih FB, Glesner J, Moon AF, Chapman MD, Tomer KB, London RE, and Pomés A
- Subjects
- Allergens genetics, Allergens immunology, Amino Acid Sequence, Animals, Cockroaches, Crystallography, X-Ray, Digestion genetics, Environmental Exposure adverse effects, Humans, Immunoglobulin E immunology, Lipids immunology, Magnetic Resonance Spectroscopy, Mice, Molecular Sequence Data, Protein Binding, Protein Conformation, Sequence Alignment, Transgenes genetics, Allergens metabolism, Asthma diagnosis, Asthma immunology, Immunoglobulin E metabolism
- Abstract
Background: Sensitization to cockroach allergens is a major risk factor for asthma. The cockroach allergen Bla g 1 has multiple repeats of approximately 100 amino acids, but the fold of the protein and its biological function are unknown., Objective: We sought to determine the structure of Bla g 1, investigate the implications for allergic disease, and standardize cockroach exposure assays., Methods: nBla g 1 and recombinant constructs were compared by using ELISA with specific murine IgG and human IgE. The structure of Bla g 1 was determined by x-ray crystallography. Mass spectrometry and nuclear magnetic resonance spectroscopy were used to examine the ligand-binding properties of the allergen., Results: The structure of an rBla g 1 construct with comparable IgE and IgG reactivity to the natural allergen was solved by x-ray crystallography. The Bla g 1 repeat forms a novel fold with 6 helices. Two repeats encapsulate a large and nearly spherical hydrophobic cavity, defining the basic structural unit. Lipids in the cavity varied depending on the allergen origin. Palmitic, oleic, and stearic acids were associated with nBla g 1 from cockroach frass. One unit of Bla g 1 was equivalent to 104 ng of allergen., Conclusions: Bla g 1 has a novel fold with a capacity to bind various lipids, which suggests a digestive function associated with nonspecific transport of lipid molecules in cockroaches. Defining the basic structural unit of Bla g 1 facilitates the standardization of assays in absolute units for the assessment of environmental allergen exposure., (Published by Mosby, Inc.)
- Published
- 2013
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37. Molecular determinants for antibody binding on group 1 house dust mite allergens.
- Author
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Chruszcz M, Pomés A, Glesner J, Vailes LD, Osinski T, Porebski PJ, Majorek KA, Heymann PW, Platts-Mills TA, Minor W, and Chapman MD
- Subjects
- Allergens genetics, Allergens immunology, Animals, Antibodies, Monoclonal, Murine-Derived genetics, Antibodies, Monoclonal, Murine-Derived immunology, Antigens, Dermatophagoides genetics, Antigens, Dermatophagoides immunology, Arthropod Proteins genetics, Arthropod Proteins immunology, Cysteine Endopeptidases genetics, Cysteine Endopeptidases immunology, Epitopes genetics, Epitopes immunology, Immunoglobulin E genetics, Immunoglobulin E immunology, Mice, Mutation, Pyroglyphidae, Vaccines chemistry, Vaccines genetics, Vaccines immunology, Allergens chemistry, Antibodies, Monoclonal, Murine-Derived chemistry, Antigens, Dermatophagoides chemistry, Arthropod Proteins chemistry, Cysteine Endopeptidases chemistry, Epitopes chemistry, Immunoglobulin E chemistry
- Abstract
House dust mites produce potent allergens, Der p 1 and Der f 1, that cause allergic sensitization and asthma. Der p 1 and Der f 1 are cysteine proteases that elicit IgE responses in 80% of mite-allergic subjects and have proinflammatory properties. Their antigenic structure is unknown. Here, we present crystal structures of natural Der p 1 and Der f 1 in complex with a monoclonal antibody, 4C1, which binds to a unique cross-reactive epitope on both allergens associated with IgE recognition. The 4C1 epitope is formed by almost identical amino acid sequences and contact residues. Mutations of the contact residues abrogate mAb 4C1 binding and reduce IgE antibody binding. These surface-exposed residues are molecular targets that can be exploited for development of recombinant allergen vaccines.
- Published
- 2012
- Full Text
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38. Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding.
- Author
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Glesner J, Wünschmann S, Li M, Gustchina A, Wlodawer A, Himly M, Chapman MD, and Pomés A
- Subjects
- Animals, Biotinylation, Circular Dichroism, Crystallography, X-Ray, Enzyme-Linked Immunosorbent Assay, Epitopes chemistry, Epitopes immunology, Fluorescence, Humans, Mutagenesis genetics, Mutant Proteins chemistry, Mutant Proteins immunology, Mutation genetics, Protein Binding immunology, Recombinant Proteins chemistry, Recombinant Proteins immunology, Antibodies, Monoclonal immunology, Antigen-Antibody Complex immunology, Aspartic Acid Endopeptidases immunology, Cockroaches immunology, Immunoglobulin E immunology
- Abstract
Background: Cockroach allergy is strongly associated with asthma, and involves the production of IgE antibodies against inhaled allergens. Reports of conformational epitopes on inhaled allergens are limited. The conformational epitopes for two specific monoclonal antibodies (mAb) that interfere with IgE antibody binding were identified by X-ray crystallography on opposite sites of the quasi-symmetrical cockroach allergen Bla g 2., Methodology/principal Findings: Mutational analysis of selected residues in both epitopes was performed based on the X-ray crystal structures of the allergen with mAb Fab/Fab' fragments, to investigate the structural basis of allergen-antibody interactions. The epitopes of Bla g 2 for the mAb 7C11 or 4C3 were mutated, and the mutants were analyzed by SDS-PAGE, circular dichroism, and/or mass spectrometry. Mutants were tested for mAb and IgE antibody binding by ELISA and fluorescent multiplex array. Single or multiple mutations of five residues from both epitopes resulted in almost complete loss of mAb binding, without affecting the overall folding of the allergen. Preventing glycosylation by mutation N268Q reduced IgE binding, indicating a role of carbohydrates in the interaction. Cation-π interactions, as well as electrostatic and hydrophobic interactions, were important for mAb and IgE antibody binding. Quantitative differences in the effects of mutations on IgE antibody binding were observed, suggesting heterogeneity in epitope recognition among cockroach allergic patients., Conclusions/significance: Analysis by site-directed mutagenesis of epitopes identified by X-ray crystallography revealed an overlap between monoclonal and IgE antibody binding sites and provided insight into the B cell repertoire to Bla g 2 and the mechanisms of allergen-antibody recognition, including involvement of carbohydrates.
- Published
- 2011
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39. Carbohydrates contribute to the interactions between cockroach allergen Bla g 2 and a monoclonal antibody.
- Author
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Li M, Gustchina A, Glesner J, Wünschmann S, Vailes LD, Chapman MD, Pomés A, and Wlodawer A
- Subjects
- Acetylglucosamine chemistry, Acetylglucosamine genetics, Allergens chemistry, Allergens genetics, Animals, Antibodies, Monoclonal chemistry, Antigen-Antibody Reactions, Aspartic Acid Endopeptidases chemistry, Aspartic Acid Endopeptidases genetics, Crystallography, X-Ray, Glycosylation, Mice, Mutagenesis, Site-Directed, Protein Binding immunology, Protein Conformation, Acetylglucosamine metabolism, Allergens metabolism, Antibodies, Monoclonal metabolism, Aspartic Acid Endopeptidases metabolism, Cockroaches immunology
- Abstract
The crystal structure of a murine mAb, 4C3, that binds to the C-terminal lobe of the cockroach allergen Bla g 2 has been solved at 1.8 Å resolution. Binding of 4C3 involves different types of molecular interactions with its epitope compared with those with the mAb 7C11, which binds to the N-terminal lobe of Bla g 2. We found that the 4C3 surface epitope on Bla g 2 includes a carbohydrate moiety attached to Asn(268) and that a large number of Ag-Ab contacts are mediated by water molecules and ions, most likely zinc. Ab binding experiments conducted with an enzymatically deglycosylated Bla g 2 and a N268Q mutant showed that the carbohydrate contributes, without being essential, to the Bla g 2-4C3 mAb interaction. Inhibition of IgE Ab binding by the mAb 4C3 shows a correlation of the structurally defined epitope with reactivity with human IgE. Site-directed mutagenesis of the 4C3 mAb epitope confirmed that the amino acids Lys(251), Glu(233), and Ile(199) are important for the recognition of Bla g 2 by the 4C3 mAb. The results show the relevance of x-ray crystallographic studies of allergen-Ab complexes to identify conformational epitopes that define the antigenic surface of Bla g 2.
- Published
- 2011
- Full Text
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40. Der p 5 crystal structure provides insight into the group 5 dust mite allergens.
- Author
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Mueller GA, Gosavi RA, Krahn JM, Edwards LL, Cuneo MJ, Glesner J, Pomés A, Chapman MD, London RE, and Pedersen LC
- Subjects
- Amino Acid Sequence, Animals, Antigens, Dermatophagoides genetics, Antigens, Dermatophagoides metabolism, Arthropod Proteins, Mites metabolism, Models, Molecular, Molecular Sequence Data, Protein Structure, Secondary, Sequence Homology, Amino Acid, Antigens, Dermatophagoides chemistry, Crystallography, X-Ray methods
- Abstract
Group 5 allergens from house dust mites elicit strong IgE antibody binding in mite-allergic patients. The structure of Der p 5 was determined by x-ray crystallography to better understand the IgE epitopes, to investigate the biologic function in mites, and to compare with the conflicting published Blo t 5 structures, designated 2JMH and 2JRK in the Protein Data Bank. Der p 5 is a three-helical bundle similar to Blo t 5, but the interactions of the helices are more similar to 2JMH than 2JRK. The crystallographic asymmetric unit contains three dimers of Der p 5 that are not exactly alike. Solution scattering techniques were used to assess the multimeric state of Der p 5 in vitro and showed that the predominant state was monomeric, similar to Blo t 5, but larger multimeric species are also present. In the crystal, the formation of the Der p 5 dimer creates a large hydrophobic cavity of approximately 3000 A(3) that could be a ligand-binding site. Many allergens are known to bind hydrophobic ligands, which are thought to stimulate the innate immune system and have adjuvant-like effects on IgE-mediated inflammatory responses.
- Published
- 2010
- Full Text
- View/download PDF
41. The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteins.
- Author
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Mueller GA, Edwards LL, Aloor JJ, Fessler MB, Glesner J, Pomés A, Chapman MD, London RE, and Pedersen LC
- Subjects
- Acute-Phase Proteins chemistry, Acute-Phase Proteins metabolism, Animals, Arthropod Proteins, Asthma etiology, Asthma immunology, Carrier Proteins chemistry, Carrier Proteins metabolism, Crystallography, X-Ray, Humans, Hypersensitivity, Immediate etiology, Magnetic Resonance Spectroscopy, Membrane Glycoproteins chemistry, Membrane Glycoproteins metabolism, Mites immunology, Toll-Like Receptor 4 metabolism, Antigens, Dermatophagoides chemistry, Antigens, Dermatophagoides immunology, Antigens, Dermatophagoides metabolism, Dust immunology, Hypersensitivity, Immediate immunology, Immunity, Innate immunology, Toll-Like Receptor 4 chemistry
- Abstract
Background: Sensitization to house dust mite allergens is strongly correlated with asthma. Der p 7 elicits strong IgE antibody and T-cell responses in patients with mite allergy. However, the structure and biological function of this important allergen are unknown. Allergen function might contribute to allergenicity, as shown for the protease activity of group 1 mite allergens and the interaction with the innate immune system by group 2 mite allergens., Objective: We sought to determine the crystal structure of Der p 7 and to investigate its biological function., Methods: X-ray crystallography was used to determine the Der p 7 structure. Nuclear magnetic resonance analysis and biochemical assays were used to examine the binding of Der p 7 to predicted ligands., Results: Der p 7 has an elongated structure, with two 4-stranded antiparallel beta-sheets that wrap around a long C-terminal helix. The fold of Der p 7 is similar to that of LPS-binding protein (LBP), which interacts with Toll-like receptors after binding LPS and other bacterially derived lipid ligands. Nuclear magnetic resonance and biochemical assays indicate that Der p 7 does not bind LPS but binds with weak affinity to the bacterial lipopeptide polymyxin B in the predicted binding site of Der p 7., Conclusions: Der p 7 binds a bacterially derived lipid product, a common feature of some allergens. The finding that the group 7, as well as the group 2, mite allergens are structurally similar to different proteins in the Toll-like receptor pathway further strengthens the connections between dust mites, innate immunity, and allergy., (Copyright (c) 2010 American Academy of Allergy, Asthma & Immunology. Published by Mosby, Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
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