Search

Your search keyword '"Gjomarkaj, Mark"' showing total 333 results
Start Over Author "Gjomarkaj, Mark"
333 results on '"Gjomarkaj, Mark"'

1. IL-17A Drives Oxidative Stress and Cell Growth in A549 Lung Epithelial Cells: Potential Protective Action of Oleuropein.

Author

Marina Montalbano, Angela, Di Sano, Caterina, Daniela Albano, Giusy, Gjomarkaj, Mark, Massimo Ricciardolo, Fabio Luigi, and Profita, Mirella

Abstract

IL-17A drives inflammation and oxidative stress, affecting the progression of chronic lung diseases (asthma, chronic obstructive pulmonary disease (COPD), lung cancer, and cystic fibrosis). Oleuropein (OLP) is a polyphenolic compound present in olive oil and widely included in the Mediterranean diet. It exerts antioxidant and anti-inflammatory activities, oxidative stress resistance, and anticarcinogenic effects with a conceivable positive impact on human health. We hypothesized that OLP positively affects the mechanisms of oxidative stress, apoptosis, DNA damage, cell viability during proliferation, and cell growth in alveolar epithelial cells and tested its effect in a human alveolar epithelial cell line (A549) in the presence of IL-17A. Our results show that OLP decreases the levels of oxidative stress (Reactive Oxygen Species, Mitochondrial membrane potential) and DNA damage (H2AX phosphorylation-ser139, Olive Tail Moment data) and increases cell apoptosis in A549 cells exposed to IL-17A. Furthermore, OLP decreases the number of viable cells during proliferation, the migratory potential (Scratch test), and the single cell capacity to grow within colonies as a cancer phenotype in A549 cells exposed to IL-17A. In conclusion, we suggest that OLP might be useful to protect lung epithelial cells from oxidative stress, DNA damage, cell growth, and cell apoptosis. This effect might be exerted in lung diseases by the downregulation of IL-17A activities. Our results suggest a positive effect of the components of olive oil on human lung health. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

3. The skin prick test ¿ European standards

Author

Heinzerling, Lucie, Mari, Adriano, Bergmann, Karl-Christian, Bresciani, Megon, Burbach, Guido, Darsow, Ulf, Durham, Stephen, Fokkens, Wytske, Gjomarkaj, Mark, Haahtela, Tari, Bom, Ana Todo, Wöhrl, Stefan, Maibach, Howard, and Lockey, Richard

Abstract

Abstract Skin prick testing is an essential test procedure to confirm sensitization in IgE-mediated allergic disease in subjects with rhinoconjunctivitis, asthma, urticaria, anapylaxis, atopic eczema and food and drug allergy. This manuscript reviews the available evidence including Medline and Embase searches, abstracts of international allergy meetings and position papers from the world allergy literature. The recommended method of prick testing includes the appropriate use of specific allergen extracts, positive and negative controls, interpretation of the tests after 15 – 20 minutes of application, with a positive result defined as a wheal ≥3 mm diameter. A standard prick test panel for Europe for inhalants is proposed and includes hazel (Corylus avellana), alder (Alnus incana), birch (Betula alba), plane (Platanus vulgaris), cypress (Cupressus sempervirens), grass mix (Poa pratensis, Dactilis glomerata, Lolium perenne, Phleum pratense, Festuca pratensis, Helictotrichon pretense), Olive (Olea europaea), mugwort (Artemisia vulgaris), ragweed (Ambrosia artemisiifolia), Alternaria alternata (tenuis), Cladosporium herbarum, Aspergillus fumigatus, Parietaria, cat, dog, Dermatophagoides pteronyssinus, Dermatophagoides farinae, and cockroach (Blatella germanica). Standardization of the skin test procedures and standard panels for different geographic locations are encouraged worldwide to permit better comparisons for diagnostic, clinical and research purposes.

Published
2013

15. Plasma proteins elevated in severe asthma despite oral steroid use and unrelated to Type-2 inflammation

Author

Mikus, Maria Sparreman, Kolmert, Johan, Andersson, Lars I., Östling, Jörgen, Knowles, Richard G., Gómez, Cristina, Ericsson, Magnus, Thörngren, John Olof, Khoonsari, Payam Emami, Dahlén, Barbro, Kupczyk, Maciej, de Meulder, Bertrand, Auffray, Charles, Bakke, Per S., Beghe, Bianca, Bel, Elisabeth H., Caruso, Massimo, Chanez, Pascal, Chawes, Bo, Fowler, Stephen J., Gaga, Mina, Geiser, Thomas, Gjomarkaj, Mark, Horváth, Ildikó, Howarth, Peter H., Johnston, Sebastian L., Joos, Guy, Krug, Norbert, Montuschi, Paolo, Musial, Jacek, Niżankowska-Mogilnicka, Ewa, Olsson, Henric K., Papi, Alberto, Rabe, Klaus F., Sandström, Thomas, Shaw, Dominick E., Siafakas, Nikolaos M., Uhlén, Mathias, Riley, John H., Bates, Stewart, Middelveld, Roelinde J.M., Wheelock, Craig E., Chung, Kian Fan, Adcock, Ian M., Sterk, Peter J., Bisgaard, Hans, Bønnelykke, Klaus, Vestbo, Jørgen, Vissing, Nadja H., and Olsson, Marianne

Abstract

Rationale Asthma phenotyping requires novel biomarker discovery. Objectives To identify plasma biomarkers associated with asthma phenotypes by application of a new proteomic panel to samples from two well-characterised cohorts of severe (SA) and mild-to-moderate (MMA) asthmatics, COPD subjects and healthy controls (HCs). Methods An antibody-based array targeting 177 proteins predominantly involved in pathways relevant to inflammation, lipid metabolism, signal transduction and extracellular matrix was applied to plasma from 525 asthmatics and HCs in the U-BIOPRED cohort, and 142 subjects with asthma and COPD from the validation cohort BIOAIR. Effects of oral corticosteroids (OCS) were determined by a 2-week, placebo-controlled OCS trial in BIOAIR, and confirmed by relation to objective OCS measures in U-BIOPRED. Results In U-BIOPRED, 110 proteins were significantly different, mostly elevated, in SA compared to MMA and HCs. 10 proteins were elevated in SA versus MMA in both U-BIOPRED and BIOAIR (alpha-1-antichymotrypsin, apolipoprotein-E, complement component 9, complement factor I, macrophage inflammatory protein-3, interleukin-6, sphingomyelin phosphodiesterase 3, TNF receptor superfamily member 11a, transforming growth factor-β and glutathione S-transferase). OCS treatment decreased most proteins, yet differences between SA and MMA remained following correction for OCS use. Consensus clustering of U-BIOPRED protein data yielded six clusters associated with asthma control, quality of life, blood neutrophils, high-sensitivity C-reactive protein and body mass index, but not Type-2 inflammatory biomarkers. The mast cell specific enzyme carboxypeptidase A3 was one major contributor to cluster differentiation. Conclusions The plasma proteomic panel revealed previously unexplored yet potentially useful Type-2independent biomarkers and validated several proteins with established involvement in the pathophysiology of SA.

Published
2022

20. Plasma proteins elevated in severe asthma despite oral steroid use and unrelated to Type-2 inflammation

Author

Sparreman Mikus, Maria, Kolmert, Johan, Andersson, Lars I, ��stling, J��rgen, Knowles, Richard G, G��mez, Cristina, Ericsson, Magnus, Th��rngren, John-Olof, Emami Khoonsari, Payam, Dahl��n, Barbro, Kupczyk, Maciej, De Meulder, Bertrand, Auffray, Charles, Bakke, Per S, Beghe, Bianca, Bel, Elisabeth H, Caruso, Massimo, Chanez, Pascal, Chawes, Bo, Fowler, Stephen J, Gaga, Mina, Geiser, Thomas, Gjomarkaj, Mark, Horv��th, Ildik��, Howarth, Peter H, Johnston, Sebastian L, Joos, Guy, Krug, Norbert, Montuschi, Paolo, Musial, Jacek, Ni��ankowska-Mogilnicka, Ewa, Olsson, Henric K, Papi, Alberto, Rabe, Klaus F, Sandstr��m, Thomas, Shaw, Dominick E, Siafakas, Nikolaos M, Uhl��n, Mathias, Riley, John H, Bates, Stewart, Middelveld, Roelinde J M, Wheelock, Craig E, Chung, Kian Fan, Adcock, Ian M, Sterk, Peter J, Djukanovic, Ratko, Nilsson, Peter, Dahl��n, Sven-Erik, James, Anna, Publica, AII - Inflammatory diseases, Pulmonology, Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
severe asthma, Inflammation, Proteomics, [SDV]Life Sciences [q-bio], Respiratory Medicine and Allergy, Socio-culturale, Plasma proteins, biomarkers, 610 Medicine & health, oral corticosteroids (OCS), Blood Proteins, Severity of Illness Index, Plasma proteins, severe asthma, chronic obstructive pulmonary disease (COPD), oral corticosteroids (OCS), biomarkers, Asthma, Quality of Life, Humans, Steroids, chronic obstructive pulmonary disease (COPD), Lungmedicin och allergi
Abstract

Rationale: Asthma phenotyping requires novel biomarker discovery.Objectives: To identify plasma biomarkers associated with asthma phenotypes by application of a new proteomic panel to samples from two well-characterised cohorts of severe (SA) and mild-to-moderate (MMA) asthmatics, COPD subjects and healthy controls (HCs).Methods: An antibody-based array targeting 177 proteins predominantly involved in pathways relevant to inflammation, lipid metabolism, signal transduction and extracellular matrix was applied to plasma from 525 asthmatics and HCs in the U-BIOPRED cohort, and 142 subjects with asthma and COPD from the validation cohort BIOAIR. Effects of oral corticosteroids (OCS) were determined by a 2-week, placebo-controlled OCS trial in BIOAIR, and confirmed by relation to objective OCS measures in U-BIOPRED.Results: In U-BIOPRED, 110 proteins were significantly different, mostly elevated, in SA compared to MMA and HCs. 10 proteins were elevated in SA versus MMA in both U-BIOPRED and BIOAIR (alpha-1-antichymotrypsin, apolipoprotein-E, complement component 9, complement factor I, macrophage inflammatory protein-3, interleukin-6, sphingomyelin phosphodiesterase 3, TNF receptor superfamily member 11a, transforming growth factor-β and glutathione S-transferase). OCS treatment decreased most proteins, yet differences between SA and MMA remained following correction for OCS use. Consensus clustering of U-BIOPRED protein data yielded six clusters associated with asthma control, quality of life, blood neutrophils, high-sensitivity C-reactive protein and body mass index, but not Type-2 inflammatory biomarkers. The mast cell specific enzyme carboxypeptidase A3 was one major contributor to cluster differentiation.Conclusions: The plasma proteomic panel revealed previously unexplored yet potentially useful Type-2-independent biomarkers and validated several proteins with established involvement in the pathophysiology of SA.

Published
2021

21. Lung function fluctuation patterns unveil asthma and COPD phenotypes unrelated to type 2 inflammation

Author

Delgado-Eckert, Edgar, primary, James, Anna, additional, Meier-Girard, Delphine, additional, Kupczyk, Maciej, additional, Andersson, Lars I., additional, Bossios, Apostolos, additional, Mikus, Maria, additional, Ono, Junya, additional, Izuhara, Kenji, additional, Middelveld, Roelinde, additional, Dahlén, Barbro, additional, Gaga, Mina, additional, Siafakas, Nikos M., additional, Papi, Alberto, additional, Beghe, Bianca, additional, Joos, Guy, additional, Rabe, Klaus F., additional, Sterk, Peter J., additional, Bel, Elisabeth H., additional, Johnston, Sebastian L., additional, Chanez, Pascal, additional, Gjomarkaj, Mark, additional, Howarth, Peter H., additional, Niżankowska-Mogilnicka, Ewa, additional, Dahlén, Sven-Erik, additional, and Frey, Urs, additional

Published
2021
Full Text
View/download PDF

22. Increased Leptin/Leptin Receptor Pathway Affects Systemic and Airway Inflammation in COPD Former Smokers [Retraction]

Author

Bruno,Andreina, Alessi,Marinella, Soresi,Simona, Bonanno,Anna, Riccobono,Loredana, Montalbano,Angela Marina, Albano,Giusy Daniela, Gjomarkaj,Mark, Profita,Mirella, Bruno,Andreina, Alessi,Marinella, Soresi,Simona, Bonanno,Anna, Riccobono,Loredana, Montalbano,Angela Marina, Albano,Giusy Daniela, Gjomarkaj,Mark, and Profita,Mirella

Abstract

Bruno A, Alessi M, Soresi S, et al. J Inflamm Res. 2011;4:51–59. The Editor and Publisher of Journal of Inflammation Research wish to retract the published article. Concerns were raised regarding the duplication of images in Figure 3 describing leptin receptor expression with those from an article published by the authors several years previously describing muscarinic receptor expression. Specifically: Figure 3A, Leptin receptor expression, Smokers, appears to have been duplicated with Figure 2 panel M3 COPD, for the expression of muscarinic receptors. Figure 3D, Leptin expression, Smokers, appears to have been duplicated with Figure 2 panel M1 Smokers, for the expression of muscarinic receptors. Figure 3A, Leptin receptor expression, Smokers, appears to share duplicated elements with Figure 3D, Leptin expression, Smokers. The authors did not respond to our queries and the Editor advised for the article to be retracted. Our decision-making was informed by our policy on publishing ethics and integrity and the COPE guidelines on retraction. The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as “Retracted”. This retraction relates to this paper

Published
2021

24. Chronic obstructive pulmonary disease and neutrophil infiltration: role of cigarette smoke and cyclooxygenase products

Author

Profita, Mirella, Sala, Angelo, Bonanno, Anna, Riccobono, Loredana, Ferraro, Maria, La Grutta, Stefania, Albano, Giusy Daniela, Montalbano, Angela Marina, and Gjomarkaj, Mark

Subjects
Lung diseases, Obstructive -- Risk factors, Lung diseases, Obstructive -- Development and progression, Lung diseases, Obstructive -- Genetic aspects, Lung diseases, Obstructive -- Research, Cigarette smoke -- Physiological aspects, Cigarette smoke -- Genetic aspects, Cyclooxygenases -- Physiological aspects, Cyclooxygenases -- Genetic aspects, Neutrophils -- Physiological aspects, Neutrophils -- Genetic aspects, Neutrophils -- Research, Biological sciences
Abstract

Cigarette smoke is the main cause of chronic obstructive pulmonary disease (COPD), where it can contribute to the observed airway inflammation. PGE2 is produced within human airways, and both pro- and anti-inflammatory activities have been reported. We quantitated [PGE.sub.2] concentrations in induced sputum supernatants from different groups of subjects and correlated the obtained values to neutrophil infiltration as well as to the expression of cyclooxygenase-2 (COX-2). Cigarette smoke extract (CSE) was used to evaluate the effect of smoking on COX-2 and [PGE.sub.2] receptor expression as well as on [PGE.sub.2] release in neutrophils and alveolar macrophages (AM) obtained from normal donors. The effects of [PGE.sub.2] and of PGE receptor agonists and antagonists were evaluated on the adhesion of neutrophil to a human bronchial epithelial cell line (16HBE). [PGE.sub.2] levels, COX-2 expression, and neutrophil infiltration were significantly higher in normal smokers and COPD smokers (P < 0.0001) compared with controls and COPD former smokers. Induced sputum supernatant caused neutrophil adhesion to 16HBE that was significantly reduced, in COPD smokers only, by [PGE.sub.2] immunoprecipitation. In vitro experiments confirmed that CSE increased [PGE.sub.2] release and COX-2 and [PGE.sub.2] receptor expression in neutrophils and AM; [PGE.sub.2] enhanced the adhesion of neutrophils to 16HBE, and a specific E-prostanoid 4 (E[P.sub.4]) receptor antagonist blunted its effect. These results suggest that CSE promote the induction of COX-2 and contributes to the proinflammatory effects of PG[E.sub.2] in the airways of COPD subjects. alveolar macrophages; neutrophils; cigarette smoke extract; cyclooxygenase-2 doi: 10.1152/ajplung.90593.2008.

Published
2010

28. Detection of exacerbations in asthma based on electronic diary data: results from the 1-year prospective BIOAIR study

Author

Kupczyk, Maciej, Haque, Shushila, Sterk, Peter J, Niżankowska-Mogilnicka, Ewa, Papi, Alberto, Bel, Elisabeth H, Chanez, Pascal, Dahlén, Barbro, Gaga, Mina, Gjomarkaj, Mark, Howarth, Peter H, Johnston, Sebastian L, Joos, Guy F, Kanniess, Frank, Tzortzaki, Eleni, James, Anna, Middelveld, Roelinde J M, Dahlén, Sven-Erik, Weersink, Els, Papadopoulos, Nikos, Oikonomidou, Erasmia, Zervas, Eleftherios, Contoli, Marco, Pauwels, Romain A., Brusselle, Guy, de Rudder, Isabelle, Schelfhout, Vanessa, Richter, Kai, Gerding, Daisy, Magnussen, Helgo, Siafakas, Nikos M., Samara, Katerina, Plataki, Maria, Papadopouli, Eva, Szczeklik, Andrzej, Ziolkowska-Graca, Bozena, Kania, Aleksander, Gawlewicz-Mroczka, Agnieszka, Duplaga, Mariusz, Figiel, Ewa, Rabe, Klaus F., Hiemstra, Pieter S., Gauw, Stefanie, van Veen, Ilonka, Kips, Johan C., Mallia, Patrick, Campbell, Deborah A, Robinson, Douglas S, Fabbri, Leo M., Romagnoli, Micaela, Vachier, Isabelle, Devautour, Catherine, Meziane, Lahouari, Maurizio Vignola, A., Pace, Elisabetta, Profita, Mirella, Holgate, Stephen T., Wilson, Susan J., Hewitt, Lorraine, Holoway, John, Damm, Katarina, Delin, Ingrid, Eduards, Marianne, Ek, Alexandra, Ekström, Tommy, Gülich, Agneta, Johansson, Lovisa E., Karlsson, Östen, Kumlin, Maria, Martling, Ingrid, and Skedinger, Maria

Published
2013
Full Text
View/download PDF

37. Airway Remodeling in Asthma*

Author

Vignola, Antonio M., Mirabella, Franco, Costanzo, Giorgio, Di Giorgi, Rossana, Gjomarkaj, Mark, Bellia, Vincenzo, and Bonsignore, Giovanni

Published
2003

39. Leptin and leptin receptor expression in asthma

Author

Bruno, Andreina, Pace, Elisabetta, Chanez, Pascal, Gras, Delphine, Vachier, Isabelle, Chiappara, Giuseppina, La Guardia, Maurizio, Gerbino, Stefania, Profita, Mirella, and Gjomarkaj, Mark

Published
2009

46. Lymphocyte apoptosis in children with central nervous system tuberculosis: a case control study

Author

Carlo Paola Di, Casuccio Alessandra, Romano Amelia, Spicola Daria, Titone Lucina, Caccamo Nadia, Dieli Francesco, Mammina Caterina, Pace Elisabetta, Gjomarkaj Mark, Melis Mario, and Tolomeo Manlio

Subjects
Pediatrics, RJ1-570
Abstract

Abstract Background Studies of the apoptosis mechanisms involved in the pathogenesis of tuberculosis have suggested that Mycobacterium tuberculosis can actively interfere with the apoptosis of infected cells. In vivo studies have been performed in adult populations but have not focused on this process in children. In the present study, we analyzed spontaneous T lymphocyte (PBT) apoptosis in the peripheral blood of children with central nervous system tuberculosis (CNS TB), before and after chemotherapy, and compared the results with healthy controls. Methods A case-control study was conducted from January 2002 to June 2009. It included 18 children with CNS TB and 17 healthy controls. Spontaneous apoptosis of PBTs, including CD4+, CD8+ and CD8+/CD28+ T cells, was evaluated after 24 and 72 h of culture in complete medium, using the Annexin V detection test. Analysis was conducted before and after chemotherapy, and expression of the apoptotic markers CD95 (Fas) and Fas ligand (FasL) was evaluated. Results Higher percentages of apoptotic T cells and CD4 lymphocytes were isolated from children with acute phase CNS TB than from children in the control group (p < 0.05). This difference significantly decreased after 60 days of specific treatment. In children with CNS TB, high levels of Fas ligand expression were detected in lymphocyte populations, associated with a high percentage of Fas positive cells, before and after treatment. In contrast to the CD4+ apoptosis profile, we did not find any significant difference in total CD8+ cell apoptosis between children with acute phase disease and the control group. However, the percentage of apoptotic CD8+/CD28+ T cells was significantly higher in the children with acute phase disease than in the healthy controls. Conclusions Our findings indicate that CNS TB in pediatric patients increases the sensitivity of CD4 and CD8+/CD28+ T cells to apoptosis, suggesting a hypoergic status of this infection. This could play a key role in the immunopathogenesis of this complicated form of TB. Interestingly, specific chemotherapy is able to normalize both apoptosis sensitivity and T-cell activation.

Published
2011
Full Text
View/download PDF

47. Mononuclear cells in exudative malignant pleural effusions: characterization of pleural phagocytic cells

Author

Gjomarkaj, Mark, Pace, Elisabetta, Melis, Mario, Spatafora, Mario, and Toews, Galen B.

Subjects
Monocytes -- Physiological aspects -- Analysis, Macrophages -- Physiological aspects -- Analysis, Pleural effusions -- Analysis -- Physiological aspects, Health, Physiological aspects, Analysis
Abstract

The aims of this study were to develop a methodology for the isolation of highly enriched mononuclear phagocyte populations from exudative malignant pleural effusions (EMPE) and to characterize the phenotype [...]

Published
1994

48. Increased expression of leukocyte function associated antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) by alveolar macrophages of patients with pulmonary sarcoidosis

Author

Melis, Mario, Gjomarkaj, Mark, Pace, Elisabetta, Malizia, Giuseppe, and Spatafora, Mario

Subjects
Antigenic determinants -- Physiological aspects, Cell adhesion molecules -- Physiological aspects, Macrophages -- Physiological aspects, Histology, Pathological -- Physiological aspects, Pulmonary alveoli -- Physiological aspects, Sarcoidosis -- Physiological aspects, Leukocytes -- Physiological aspects, Health, Physiological aspects
Abstract

Leukocyte function associated antigen-1 (LFA-1) and its ligand intercellular adhesion molecule-1 (ICAM-1) are cell adhesion molecules that play an important role in the capacity of mononuclear phagocytes (MPs) to present [...]

Published
1991

49. Cigarette smoke and non‐neuronal cholinergic system in the airway epithelium of COPD patients

Author

Montalbano, Angela M., primary, Di Sano, Caterina, additional, Chiappara, Giuseppina, additional, Riccobono, Loredana, additional, Bonanno, Anna, additional, Anzalone, Giulia, additional, Vitulo, Patrizio, additional, Pipitone, Loredana, additional, Gjomarkaj, Mark, additional, Pieper, Michael P., additional, Ricciardolo, Fabio L. M., additional, Gagliardo, Rosalia P., additional, and Profita, Mirella, additional

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results