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39 results on '"Giuffrida, Domenica"'

9. Altered Expression of G1/S Phase Cell Cycle Regulators in Placental Mesenchymal Stromal Cells Derived from Preeclamptic Pregnancies with Fetal-Placental Compromise

Author

Nuzzo, Anna Maria, Giuffrida, Domenica, Masturzo, Bianca, Mele, Paolo, Piccoli, Ettore, Eva, Carola, Todros, Tullia, and Rolfo, Alessandro

Subjects
endocrine system
Abstract

Herein, we evaluated whether Placental Mesenchymal Stromal Cells (PDMSCs) derived from normal and Preeclamptic (PE) placentae presented differences in the expression of G1/S-phase regulators p16INK4A, p18INK4C, CDK4 and CDK6. Finally, we investigated normal and PE-PDMSCs paracrine effects on JunB, Cyclin D1, p16INK4A, p18INK4C, CDK4 and CDK6 expressions in physiological term villous explants. PDMSCs were isolated from physiological (n = 20) and PE (n = 24) placentae. Passage three normal and PE-PDMSC and conditioned media (CM) were collected after 48 h. Physiological villous explants (n = 60) were treated for 72 h with normal or PE-PDMSCs CM. Explants viability was assessed by Lactate Dehydrogenase Cytotoxicity assay. Cyclin D1 localization was evaluated by Immuofluorescence (IF) while JunB, Cyclin-D1 p16INK4A, p18INK4C, CDK4 and CDK6 levels were assessed by Real Time PCR and Western Blot assay. We reported significantly increased p16INK4A and p18INK4C expression in PE- relative to normal PDMSCs while no differences in CDK4 and CDK6 levels were detected. Explants viability was not affected by normal or PE-PDMSCs CM. Normal PDMSCs CM increased JunB, p16INK4 and p18INK4C and decreased Cyclin-D1 in placental tissues. In contrast, PE-PDMSCs CM induced JunB down-regulation and Cyclin D1 increase in placental explants. Cyclin D1 IF staining showed that CM treatment targeted mainly the syncytiotrophoblast. We showed Cyclin D1-p16INK4A/p18INK4C altered pathway in PE-PDMSCs demonstrating an aberrant G1/S phase transition in these pathological cells. The abnormal Cyclin D1-p16INK4A/p18INK4C expression in explants conditioned by PE-PDMSCs media suggest a key contribution of mesenchymal cells to the altered trophoblast cell cycle regulation typical of PE pregnancies with fetal-placental compromise.

Published
2016
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13. Low protein diets in patients with chronic kidney disease: a bridge between mainstream and complementary-alternative medicines?

Author

Piccoli, Giorgina Barbara, primary, Capizzi, Irene, additional, Vigotti, Federica Neve, additional, Leone, Filomena, additional, D’Alessandro, Claudia, additional, Giuffrida, Domenica, additional, Nazha, Marta, additional, Roggero, Simona, additional, Colombi, Nicoletta, additional, Mauro, Giuseppe, additional, Castelluccia, Natascia, additional, Cupisti, Adamasco, additional, and Avagnina, Paolo, additional

Published
2016
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14. Pregnancy in dialysis patients in the new millennium: a systematic review and meta-regression analysis correlating dialysis schedules and pregnancy outcomes

Author

Piccoli, Giorgina Barbara, primary, Minelli, Fosca, additional, Versino, Elisabetta, additional, Cabiddu, Gianfranca, additional, Attini, Rossella, additional, Vigotti, Federica Neve, additional, Rolfo, Alessandro, additional, Giuffrida, Domenica, additional, Colombi, Nicoletta, additional, Pani, Antonello, additional, and Todros, Tullia, additional

Published
2015
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19. Is It Possible to Differentiate Chronic Kidney Disease and Preeclampsia by means of New and Old Biomarkers? A Prospective Study

Author

Rolfo, Alessandro, primary, Attini, Rossella, additional, Tavassoli, Elisabetta, additional, Vigotti Neve, Federica, additional, Nigra, Marco, additional, Cicilano, Matteo, additional, Nuzzo, Anna Maria, additional, Giuffrida, Domenica, additional, Biolcati, Marilisa, additional, Nichelatti, Michele, additional, Gaglioti, Pietro, additional, Todros, Tullia, additional, and Piccoli, Giorgina Barbara, additional

Published
2015
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22. Pregnancy in dialysis patients in the new millennium: a systematic review and meta-regression analysis correlating dialysis schedules and pregnancy outcomes.

Author

Piccoli, Giorgina Barbara, Minelli, Fosca, Versino, Elisabetta, Cabiddu, Gianfranca, Attini, Rossella, Vigotti, Federica Neve, Rolfo, Alessandro, Giuffrida, Domenica, Colombi, Nicoletta, Pani, Antonello, and Todros, Tullia

Subjects
PREGNANCY, HEMODIALYSIS, PREMATURE labor, GESTATIONAL age, HUMAN abnormalities
Abstract

Background. Advances have been made in the management of pregnancies in women receiving dialysis; however, singlecentre studies and small numbers of cases have so far precluded a clear definition of the relationship between dialysis schedules and pregnancy outcomes. The aim of the present systematic review was to analyse the relationship between dialysis schedule and pregnancy outcomes in pregnancies in chronic dialysis in the new millennium. Methods. Medline-PubMed, Embase and the Cochrane library were searched (1 January 2000-31 December 2014: MESH, Emtree, free terms on pregnancy and dialysis). A separate analysis was performed for case series (more than five cases) and case reports. Meta-regression was performed in case series dealing with the larger subset of haemodialysis (HD) patients; case reports were analysed separately [according to peritoneal dialysis (PD) versus HD; conception before or during dialysis]. Results. We obtained 190 full texts and 25 congress abstracts from 2048 references. We selected 101 full papers and 25 abstracts (36 series; 90 case reports), for a total of 681 pregnancies in 647 patients. In the case series (574 pregnancies in 543 patients), preterm delivery was extremely frequent (83%). Meta-regression analysis showed a relationship between hours of dialysis per week inHDand preterm delivery, and was significant for preterm deliveries (<37 gestational weeks: P = 0.044; r² = 0.22) and for small for gestational age (SGA) (P = 0.017; r² = 0.54). SGA was closely associated with the number of dialysis sessions per week (P = 0.003; r² = 0.84). Case report analysis suggests a lower incidence of SGA on HD versus PD (31 versus 66.7%; P = 0.015). No evidence of an increased risk of congenital abnormality was found in the retrieved papers. Conclusions. Data on pregnancy on dialysis are heterogeneous but rapidly accumulating; the main determinant of outcomes on HD is the dialysis schedule. The differences between PD and HD should be further analysed. [ABSTRACT FROM AUTHOR]

Published
2016
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23. Characterization of DUFFY Decoy Receptor in Placentae from Pregnancies Complicated by both Preeclampsia and Gestational Diabetes

Author

Giuffrida Domenica, Todros Tullia, Zenerino Cristian, Rolfo Alessandro, and Nuzzo Anna Maria

Subjects
medicine.medical_specialty, business.industry, Obstetrics, Placenta, Obstetrics and Gynecology, gestational diabetes mellitus, Preeclampsia, Decoy Receptors, DUFFY, medicine.disease, Gestational diabetes, medicine.anatomical_structure, Endocrinology, Reproductive Medicine, Internal medicine, medicine, Decoy receptors, Receptor, business, Decoy, Developmental Biology
Published
2013

26. Association of Low-Protein Supplemented Diets with Fetal Growth in Pregnant Women with CKD

Author

Piccoli, Giorgina B., primary, Leone, Filomena, additional, Attini, Rossella, additional, Parisi, Silvia, additional, Fassio, Federica, additional, Deagostini, Maria Chiara, additional, Ferraresi, Martina, additional, Clari, Roberta, additional, Ghiotto, Sara, additional, Biolcati, Marilisa, additional, Giuffrida, Domenica, additional, Rolfo, Alessandro, additional, and Todros, Tullia, additional

Published
2014
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31. Severe Diabetic Nephropathy in Type 1 Diabetes and Pregnancy - A Case Series

Author

Piccoli, Giorgina B., primary, Tavassoli, Elisabetta, additional, Melluzza, Carmela, additional, Grassi, Giorgio, additional, Monzeglio, Clara, additional, Donvito, Valentina, additional, Leone, Filomena, additional, Attini, Rossella, additional, Ghiotto, Sara, additional, Clari, Roberta, additional, Moro, Irene, additional, Fassio, Federica, additional, Parisi, Silvia, additional, Pilloni, Eleonora, additional, Vigotti, Federica N., additional, Giuffrida, Domenica, additional, Rolfo, Alessandro, additional, and Todros, Tullia, additional

Published
2013
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33. Three apoptotic genes are upregulated in a patient with Alzheimer's disease and well-differentiated squamous cell carcinoma

Author

Salemi, Michele, primary, Giuffrida, Domenica, additional, Giuffrida, Maria C., additional, Soma, Pier Franco, additional, La Vignera, Sandro, additional, Cimino, Laura, additional, Condorelli, Rosita A., additional, Romano, Carmelo, additional, Bosco, Paolo, additional, Vicari, Lucia O., additional, and Calogero, Aldo E., additional

Published
2012
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36. New perspectives for prostate cancer treatment: in vitro inhibition of LNCaP and PC3 cell proliferation by amnion-derived mesenchymal stromal cells conditioned media.

Author

Rolfo, Alessandro, Giuffrida, Domenica, Giuffrida, Maria C., Todros, Tullia, and Calogero, Aldo E.

Subjects
*PROSTATE cancer treatment, *AMNION, *MESENCHYMAL stem cells, *CELL proliferation, *CANCER cells, *PROPIDIUM iodide
Abstract

Aim: To determine whether normal human amnion-derived mesenchymal stromal cells (hAMSCs) secrete trophic mediators able to inhibit human prostate cancer cell lines growth. Methods: Human prostate cancer and normal cell lines were used. Mesenchymal stromal cells (MSC) were isolated through mechanical and enzymatic digestion from amniotic membranes and were evaluated for specific mesenchymal stromal cells antigens. Cell proliferation was examined by MTT assay. Staining with propidium iodide (PI) followed by flow cytometry was used to detect cell cycle phase. Results: hAMSC showed proper mesenchymal stem cells phenotype. We found that hAMSC conditioned media (CM) inhibited prostate cancer cells proliferation. Indeed, we demonstrated that hAMSC CM treatment increased percentage of G1 cancer cells and decreased percentage of cancer cells in S and G2M phases, suggesting that the hAMSC factors slow progression of prostate cancer cells through cell cycle inhibition. Conclusions: Our study provide evidences that hAMSC microenvironment secretes soluble factors able to inhibit prostate cancer cells growth. This may represent a novel strategy to control proliferation of prostate cancer through modulation of the host microenvironment. [ABSTRACT FROM AUTHOR]

Published
2014
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39. [Intensive weight-loss in dialysis: a personalized approach].

Author

Vigotti FN, Guzzo G, Capizzi I, Teta L, Ippolito D, Mirasole S, Giuffrida D, Avagnina P, and Piccoli G

Subjects
Humans, Kidney Failure, Chronic complications, Kidney Failure, Chronic therapy, Male, Middle Aged, Obesity complications, Diet, Reducing, Obesity therapy, Renal Dialysis, Weight Loss
Abstract

Unlabelled: Obesity is increasingly encountered in dialysis patients, who have difficulty to lose weight. Several Transplant Centres require BMI <30-35 Kg/m2 at waiting-list. Thus, losing weight becomes a must for young obese patients, however the best policy to obtain it (if any) is not defined. The aim of the present case report is to suggest that tailored dialysis and intensive diets could be a successful combination, that should be tested on a larger scale. A 56-year-old obese male patient (BMI 37.7 kg/m²) on daily home hemodialysis since 10 months (ESRD due to focal segmental glomerulosclerosis) started a coach-assisted qualitative ad libitum diet. The diet, alternating 8 weeks of rapid weight loss and maintenance phases, was based on a combinations of different foods, chosen on the account of glycaemic index and biochemical properties. It was salt free and olive oil was permitted in liberal quantities. Dialysis duration was increased to allow weight loss, and dialysate Na was incremented to permit a strict low sodium diet. Over a period of 21 months, the patient attained a -18.5 Kg weight loss (50% overweight loss; BMI -6.3 Kg/m²), reaching the goal to be included in a kidney transplant waiting list. Main metabolic data remained stable (pre diet and end of the diet period: albumin 3.5-3.8 g/dL; HCO3 26.1-24.8 mmol/L discontinuing citrate) or improved (haemoglobin 11.4-12.1 g/dL, halving EPO dose; calcium 2.3-2.5 mmol/L; phosphate 1.5-1.5 mmol/L; PTHi 1718-251 pg/mL, reducing chelation)., Conclusion: Daily dialysis may allow enrolling obese hemodialysis patients in intensive weight loss programs, under strict clinical control.

Published
2014

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