607 results on '"Giudetti, A"'
Search Results
2. M1-derived extracellular vesicles polarize recipient macrophages into M2-like macrophages and alter skeletal muscle homeostasis in a hyper-glucose environment
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Stefano Tacconi, Francesco Vari, Carolina Sbarigia, Diana Vardanyan, Serena Longo, Francesco Mura, Federica Angilè, Audrey Jalabert, Ferninand Blangero, Assia Eljaafari, Laurence Canaple, Daniele Vergara, Francesco Paolo Fanizzi, Marco Rossi, Claire Crola Da Silva, Elizabeth Errazuriz-Cerda, Christel Cassin, Rienk Nieuwland, Anna Maria Giudetti, Sophie Rome, and Luciana Dini
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Extracellular vesicles ,Hyperglycemia ,Macrophage ,Lipid metabolism ,Skeletal muscle ,Oxidative phosphorylation ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background Macrophages release not only cytokines but also extracellular vesicles (EVs). which are small membrane-derived nanovesicles with virus-like properties transferring cellular material between cells. Until now, the consequences of macrophage plasticity on the release and the composition of EVs have been poorly explored. In this study, we determined the impact of high-glucose (HG) concentrations on macrophage metabolism, and characterized their derived-EV subpopulations. Finally, we determined whether HG-treated macrophage-derived EVs participate in immune responses and in metabolic alterations of skeletal muscle cells. Methods THP1-macrophages were treated with 15mM (MG15) or 30mM (MG30) glucose. Then, M1/M2 canonical markers, pro- and anti-inflammatory cytokines, activities of proteins involved in glycolysis or oxidative phosphorylation were evaluated. Macrophage-derived EVs were characterized by TEM, NTA, MRSP, and 1H-Nuclear magnetic resonance spectroscopy for lipid composition. Macrophages or C2C12 muscle cells were used as recipients of MG15 and MG30-derived EVs. The lipid profiles of recipient cells were determined, as well as proteins and mRNA levels of relevant genes for macrophage polarization or muscle metabolism. Results Untreated macrophages released small and large EVs (sEVs, lEVs) with different lipid distributions. Proportionally to the glucose concentration, glycolysis was induced in macrophages, associated to mitochondrial dysfunction, triacylglycerol and cholesterol accumulation. In addition, MG15 and MG30 macrophages had increased level of CD86 and increase release of pro-inflammatory cytokines. HG also affected macrophage sphingolipid and phospholipid compositions. The differences in the lipid profiles between sEVs and lEVs were abolished and reflected the lipid alterations in MG15 and MG30 macrophages. Interestingly, MG15 and MG30 macrophages EVs induced the expression of CD163, Il-10 and increased the contents of triacylglycerol and cholesterol in recipient macrophages. MG15 lEVs and sEVs induced insulin-induced AKT hyper-phosphorylation and accumulation of triacylglycerol in myotubes, a state observed in pre-diabetes. Conversely, MG30 lEVs and sEVs induced insulin-resistance in myotubes. Conclusions As inflammation involves first M1 macrophages, then the activation of M2 macrophages to resolve inflammation, this study demonstrates that the dialog between macrophages through the EV route is an intrinsic part of the inflammatory response. In a hyperglycemic context, EV macrophages could participate in the development of muscle insulin-resistance and chronic inflammation.
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- 2024
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3. Bioimpedentiometry parameters used as indicators of frailty and malnutrition: association between G8 score and Phase angle (PHA) in elderly cancer patients
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Leo, Silvana, Marinelli, Fabiana, Zurlo, Ina Valeria, Guarini, Vincenzo, Accettura, Caterina, Falco, Andrea, Leone, Stefania, Saracino, Valeria, Giudetti, Anna Maria, and Piscitelli, Prisco
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- 2023
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4. EPA and DHA Enhance CACT Promoter Activity by GABP/NRF2
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Eleonora Stanca, Francesco Spedicato, Anna Maria Giudetti, Laura Giannotti, Benedetta Di Chiara Stanca, Fabrizio Damiano, and Luisa Siculella
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CACT ,GABP/NRF2 ,n-3 PUFA ,fatty acid β-oxidation ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Carnitine-acylcarnitine translocase (CACT) is a nuclear-encoded mitochondrial carrier that catalyzes the transfer of long-chain fatty acids across the inner mitochondrial membrane for β-oxidation. In this study, we conducted a structural and functional characterization of the CACT promoter to investigate the molecular mechanism underlying the transcriptional regulation of the CACT gene by n-3 PUFA, EPA and DHA. In hepatic BRL3A cells, EPA and DHA stimulate CACT mRNA and protein expression. Deletion promoter analysis using a luciferase reporter gene assay identified a n-3 PUFA response region extending from −202 to −29 bp. This region did not contain a response element for PPARα, a well-known PUFA-responsive nuclear receptor. Instead, bioinformatic analysis revealed two highly conserved GABP responsive elements within this region. Overexpression of GABPα and GABPβ subunits, but not PPARα, increased CACT promoter activity, more remarkably upon treatment with EPA and DHA. ChIP assays showed that n3-PUFA enhanced the binding of GABPα to the −202/−29 bp sequence. Furthermore, both EPA and DHA induced nuclear accumulation of GABPα. In conclusion, our findings indicate that the upregulation of CACT by n3-PUFA in hepatic cells is independent from PPARα and could be mediated by GABP activation.
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- 2024
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5. Activity-Based Prospective Memory in Insomniacs
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Miranda Occhionero, Lorenzo Tonetti, Federica Giudetti, and Vincenzo Natale
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actigraphy ,insomnia ,prospective memory ,sleep ,Chemical technology ,TP1-1185 - Abstract
Objective: To investigate the activity-based prospective memory performance in patients with insomnia, divided, on the basis of actigraphic evaluation, into sleep onset, maintenance, mixed and negative misperception insomnia. Methods: A total of 153 patients with insomnia (I, 83 females, mean age + SD = 41.37 + 16.19 years) and 121 healthy controls (HC, 78 females, mean age + SD = 36.99 + 14.91 years) wore an actigraph for one week. Insomnia was classified into sleep onset insomnia (SOI), maintenance insomnia (MaI), mixed insomnia (MixI) and negative misperception insomnia (NMI). To study their activity-based prospective memory performance, all the participants were required to push the actigraph event marker button twice, at bedtime (task 1) and at get-up time (task 2). Results: Only patients with maintenance and mixed insomnia had a significantly lower accuracy in the activity-based prospective memory task at get-up time compared with the healthy controls. Conclusion: The results show that maintenance and mixed insomnia involve an impaired activity-based prospective memory performance, while sleep onset and negative misperception insomnia do not seem to be affected. This pattern of results suggests that the fragmentation of sleep may play a role in activity-based prospective memory efficiency at wake-up in the morning.
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- 2024
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6. Anti-nuclear autoantibodies in Graves’ disease and Graves’ orbitopathy
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Lanzolla, G., Puccinelli, L., Giudetti, M., Comi, S., Menconi, F., Maglionico, M. N., Posarelli, C., Figus, M., Marcocci, C., and Marinò, M.
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- 2023
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7. Editorial: Lipid metabolism in obesity
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Anna Maria Giudetti
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lipids ,metabolism ,obesity ,diabetes ,non-alcoholic fatty liver disease ,Physiology ,QP1-981 - Published
- 2023
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8. Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation
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Angelica Giuliani, Anna Maria Giudetti, Daniele Vergara, Laura Del Coco, Deborah Ramini, Sara Caccese, Matilde Sbriscia, Laura Graciotti, Gianluca Fulgenzi, Luca Tiano, Francesco Paolo Fanizzi, Fabiola Olivieri, Maria Rita Rippo, and Jacopo Sabbatinelli
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endothelial cells ,senescence ,fatty acid oxidation ,senescence-associated secretory phenotype ,glycolysis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Cellular senescence is closely linked to endothelial dysfunction, a key factor in age-related vascular diseases. Senescent endothelial cells exhibit a proinflammatory phenotype known as SASP, leading to chronic inflammation (inflammaging) and vascular impairments. Albeit in a state of permanent growth arrest, senescent cells paradoxically display a high metabolic activity. The relationship between metabolism and inflammation is complex and varies across cell types and senescence inductions. While some cell types shift towards glycolysis during senescence, others favor oxidative phosphorylation (OXPHOS). Despite the high availability of oxygen, quiescent endothelial cells (ECs) tend to rely on glycolysis for their bioenergetic needs. However, there are limited data on the metabolic behavior of senescent ECs. Here, we characterized the metabolic profiles of young and senescent human umbilical vein endothelial cells (HUVECs) to establish a possible link between the metabolic status and the proinflammatory phenotype of senescent ECs. Senescent ECs internalize a smaller amount of glucose, have a lower glycolytic rate, and produce/release less lactate than younger cells. On the other hand, an increased fatty acid oxidation activity was observed in senescent HUVECs, together with a greater intracellular content of ATP. Interestingly, blockade of glycolysis with 2-deoxy-D-glucose in young cells resulted in enhanced production of proinflammatory cytokines, while the inhibition of carnitine palmitoyltransferase 1 (CPT1), a key rate-limiting enzyme of fatty acid oxidation, ameliorated the SASP in senescent ECs. In summary, metabolic changes in senescent ECs are complex, and this research seeks to uncover potential strategies for modulating these metabolic pathways to influence the SASP.
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- 2023
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9. Exploring the Global Reaction Coordinate for Retinal Photoisomerization: A Graph Theory-Based Machine Learning Approach.
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Giudetti, Goran, Mukherjee, Madhubani, Nandi, Samprita, Agrawal, Sraddha, Prezhdo, Oleg V., and Nakano, Aiichiro
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- 2024
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10. EPA and DHA Enhance CACT Promoter Activity by GABP/NRF2.
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Stanca, Eleonora, Spedicato, Francesco, Giudetti, Anna Maria, Giannotti, Laura, Di Chiara Stanca, Benedetta, Damiano, Fabrizio, and Siculella, Luisa
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OMEGA-3 fatty acids ,LIVER cells ,GENE expression ,GENETIC transcription regulation ,GENETIC regulation - Abstract
Carnitine-acylcarnitine translocase (CACT) is a nuclear-encoded mitochondrial carrier that catalyzes the transfer of long-chain fatty acids across the inner mitochondrial membrane for β-oxidation. In this study, we conducted a structural and functional characterization of the CACT promoter to investigate the molecular mechanism underlying the transcriptional regulation of the CACT gene by n-3 PUFA, EPA and DHA. In hepatic BRL3A cells, EPA and DHA stimulate CACT mRNA and protein expression. Deletion promoter analysis using a luciferase reporter gene assay identified a n-3 PUFA response region extending from −202 to −29 bp. This region did not contain a response element for PPARα, a well-known PUFA-responsive nuclear receptor. Instead, bioinformatic analysis revealed two highly conserved GABP responsive elements within this region. Overexpression of GABPα and GABPβ subunits, but not PPARα, increased CACT promoter activity, more remarkably upon treatment with EPA and DHA. ChIP assays showed that n3-PUFA enhanced the binding of GABPα to the −202/−29 bp sequence. Furthermore, both EPA and DHA induced nuclear accumulation of GABPα. In conclusion, our findings indicate that the upregulation of CACT by n3-PUFA in hepatic cells is independent from PPARα and could be mediated by GABP activation. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Androgen receptor in breast cancer: The '5W' questions
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Sara Ravaioli, Roberta Maltoni, Barbara Pasculli, Paola Parrella, Anna Maria Giudetti, Daniele Vergara, Maria Maddalena Tumedei, Francesca Pirini, and Sara Bravaccini
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anti-AR therapy ,AR/ER ratio ,AR biomarker ,AR signaling ,breast cancer ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Androgen receptor (AR) is expressed in 60-70% of breast cancers (BCs) and the availability of anti-AR compounds, currently used for treating prostate cancer, paves the way to tackle specifically AR-positive BC patients. The prognostic and predictive role of AR in BC is a matter of debate, since the results from clinical trials are not striking, probably due to both technical and biological reasons. In this review, we aimed to highlight WHAT is AR, describing its structure and functions, WHAT to test and HOW to detect AR, WHERE AR should be tested (on primary tumor or metastasis) and WHY studying this fascinating hormone receptor, exploring and debating on its prognostic and predictive role. We considered AR and its ratio with other hormone receptors, analyzing also studies including patients with ductal carcinoma in situ and with early and advanced BC, as well. We also emphasized the effects that both other hormone receptors and the newly emerging androgen-inducible non coding RNAs may have on AR function in BC pathology and the putative implementation in the clinical setting. Moreover, we pointed out the latest results by clinical trials and we speculated about the use of anti-AR therapies in BC clinical practice.
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- 2022
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12. Simulations Across Scales: Insights into Biomolecular Mechanisms, Magnetic Materials, and Optical Processes
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Giudetti, Goran and Giudetti, Goran
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This thesis showcases different applications of computational and theoretical chemistry in the field of drug design, material development, and photochemistry. A computational protocol based on QM/MM methods is benchmarked and subsequently applied to model the inhibition of the SARS-CoV-2 Main Protease by the action of 4 inhibitors via 3 different reaction mechanisms. Force field parameters for azobenzene molecules embedded in a DNA G-quadruplex are developed for modeling the stability of the overall system with classical molecular dynamics simulations. Nickelocene molecular magnets are modelled on top of an MgO surface within an electrostatic embedding approach to evaluate the preservation of the spin properties of these molecules upon adsorption on a metal surface.
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- 2024
13. Neural Correlates of Antisocial Behavior: The Victim’s Perspective
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Nicolò Trevisan, Giulia Cattarinussi, Daniele Olivo, Andrea Di Ciano, Lucia Giudetti, Alan Pampallona, Katharina M. Kubera, Dusan Hirjak, Robert Christian Wolf, and Fabio Sambataro
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antisocial behavior ,self-centered behavior ,social interaction ,functional magnetic resonance imaging ,brain activation ,functional connectivity ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Antisocial behavior involves actions that disregard the basic rights of others and may represent a threat to the social system. The neural processes associated with being subject to antisocial behavior, including social victimization, are still unknown. In this study, we used a social interaction task during functional magnetic resonance imaging to investigate the neural bases of social victimization. Brain activation and functional connectivity (FC) were estimated and correlated with the Big 5 Questionnaire, Temperament Evaluation in Memphis, Pisa and San Diego (TEMPS-M), and a Questionnaire of Daily Frustration scores. During social victimization, the right occipital and temporal cortex showed increased activation. The temporal cortex also had reduced FC with homotopic areas. Compared to the prosocial interaction, social victimization showed hyperactivation of the dorsomedial and lateral prefrontal cortex, putamen, and thalamus and increased FC of the medial-frontal–striatal–thalamic areas with the ventrolateral prefrontal cortex, insula, dorsal cingulate, and postcentral gyrus. Lastly, neuroticism, irritable temperament, and frustration scores were correlated with the magnitude of neural responses to social victimization. Our findings suggest that social victimization engages a set of regions associated with salience, emotional processing, and regulation, and these responses can be modulated by temperamental and personality traits.
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- 2023
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14. Modulation of the Circulating Extracellular Vesicles in Response to Different Exercise Regimens and Study of Their Inflammatory Effects
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Serena Maggio, Barbara Canonico, Paola Ceccaroli, Emanuela Polidori, Andrea Cioccoloni, Luca Giacomelli, Carlo Ferri Marini, Giosuè Annibalini, Marco Gervasi, Piero Benelli, Francesco Fabbri, Laura Del Coco, Francesco Paolo Fanizzi, Anna Maria Giudetti, Francesco Lucertini, and Michele Guescini
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extracellular vesicles ,aerobic exercise ,aerobic training ,exerkines ,myokines ,miRNA expression ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Exercise-released extracellular vesicles (EVs) are emerging as a novel class of exerkines that promotes systemic beneficial effects. However, slight differences in the applied exercise protocols in terms of mode, intensity and duration, as well as the need for standardized protocols for EV isolation, make the comparison of the studies in the literature extremely difficult. This work aims to investigate the EV amount and EV-associated miRNAs released in circulation in response to different physical exercise regimens. Healthy individuals were subjected to different exercise protocols: acute aerobic exercise (AAE) and training (AT), acute maximal aerobic exercise (AMAE) and altitude aerobic training (AAT). We found a tendency for total EVs to increase in the sedentary condition compared to trained participants following AAE. Moreover, the cytofluorimetric analysis showed an increase in CD81+/SGCA+/CD45− EVs in response to AAE. Although a single bout of moderate/maximal exercise did not impact the total EV number, EV-miRNA levels were affected as a result. In detail, EV-associated miR-206, miR-133b and miR-146a were upregulated following AAE, and this trend appeared intensity-dependent. Finally, THP-1 macrophage treatment with exercise-derived EVs induced an increase of the mRNAs encoding for IL-1β, IL-6 and CD163 using baseline and immediately post-exercise EVs. Still, 1 h post-exercise EVs failed to stimulate a pro-inflammatory program. In conclusion, the reported data provide a better understanding of the release of circulating EVs and their role as mediators of the inflammatory processes associated with exercise.
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- 2023
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15. Activity-Based Prospective Memory in Insomniacs.
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Occhionero, Miranda, Tonetti, Lorenzo, Giudetti, Federica, and Natale, Vincenzo
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PROSPECTIVE memory ,INSOMNIACS ,INSOMNIA - Abstract
Objective: To investigate the activity-based prospective memory performance in patients with insomnia, divided, on the basis of actigraphic evaluation, into sleep onset, maintenance, mixed and negative misperception insomnia. Methods: A total of 153 patients with insomnia (I, 83 females, mean age + SD = 41.37 + 16.19 years) and 121 healthy controls (HC, 78 females, mean age + SD = 36.99 + 14.91 years) wore an actigraph for one week. Insomnia was classified into sleep onset insomnia (SOI), maintenance insomnia (MaI), mixed insomnia (MixI) and negative misperception insomnia (NMI). To study their activity-based prospective memory performance, all the participants were required to push the actigraph event marker button twice, at bedtime (task 1) and at get-up time (task 2). Results: Only patients with maintenance and mixed insomnia had a significantly lower accuracy in the activity-based prospective memory task at get-up time compared with the healthy controls. Conclusion: The results show that maintenance and mixed insomnia involve an impaired activity-based prospective memory performance, while sleep onset and negative misperception insomnia do not seem to be affected. This pattern of results suggests that the fragmentation of sleep may play a role in activity-based prospective memory efficiency at wake-up in the morning. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Kdm7a expression is spatiotemporally regulated in developing Xenopus laevis embryos, and its overexpression influences late retinal development.
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Martini, Davide, Digregorio, Matteo, Voto, Ilaria Anna Pia, Morabito, Giuseppe, Degl'Innocenti, Andrea, Giudetti, Guido, Giannaccini, Martina, and Andreazzoli, Massimiliano
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GENE expression ,XENOPUS laevis ,GENETIC overexpression ,HISTONE demethylases ,CENTRAL nervous system - Abstract
Background: Post‐translational histone modifications are among the most common epigenetic modifications that orchestrate gene expression, playing a pivotal role during embryonic development and in various pathological conditions. Among histone lysine demethylases, KDM7A, also known as KIAA1718 or JHDM1D, catalyzes the demethylation of H3K9me1/2 and H3K27me1/2, leading to transcriptional regulation. Previous data suggest that KDM7A plays a central role in several biological processes, including cell proliferation, commitment, differentiation, apoptosis, and maintenance. However, information on the expression pattern of KDM7A in whole organisms is limited, and its functional role is still unclear. Results: In Xenopus development, kdm7a is expressed early, undergoing spatiotemporal regulation in various organs and tissues, including the central nervous system and the eye. Focusing on retinal development, we found that kdm7a overexpression does not affect the expression of genes critically involved in early neural development and eye‐field specification, whereas unbalances the distribution of neural cell subtypes in the mature retina by disfavoring the development of ganglion cells while promoting that of horizontal cells. Conclusions: Kdm7a is dynamically expressed during embryonic development, and its overexpression influences late retinal development, suggesting a potential involvement in the molecular machinery regulating the spatiotemporally ordered generation of retinal neuronal subtypes. Key Findings: Histone methylation are among the most common epigenetic modifications that orchestrate gene expression.Although the histone demethylases kdm7a has been involved in various biological processes, data about its expression pattern and functional role during embryogenesis are still limited.Herein, we analyze the spatio‐temporal expression pattern of kdm7a in Xenopus laevis development and found that kdm7a overexpression specifically affects retinogenesis by disfavouring the development of ganglion cells while promoting that of horizontal cells. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Editorial: Lipid Orchestrated Signaling in Physiology and Pathology
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Anna Maria Giudetti, Pasquale Simeone, Piero Del Boccio, and Daniele Vergara
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lipid ,signaling ,metabolism ,physiology ,pathology ,Physiology ,QP1-981 - Published
- 2022
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18. Exploring the Global Reaction Coordinate for Retinal Photoisomerization: A Graph Theory-Based Machine Learning Approach
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Giudetti, Goran, primary, Mukherjee, Madhubani, additional, Nandi, Samprita, additional, Agrawal, Sraddha, additional, Prezhdo, Oleg, additional, and Nakano, Aiichiro, additional
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- 2023
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19. Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation
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Giuliani, Angelica, primary, Giudetti, Anna Maria, additional, Vergara, Daniele, additional, Del Coco, Laura, additional, Ramini, Deborah, additional, Caccese, Sara, additional, Sbriscia, Matilde, additional, Graciotti, Laura, additional, Fulgenzi, Gianluca, additional, Tiano, Luca, additional, Fanizzi, Francesco Paolo, additional, Olivieri, Fabiola, additional, Rippo, Maria Rita, additional, and Sabbatinelli, Jacopo, additional
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- 2023
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20. Kdm7a expression is spatiotemporally regulated in developing Xenopus laevis embryos, and its overexpression influences late retinal development
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Martini, Davide, primary, Digregorio, Matteo, additional, Voto, Ilaria Anna Pia, additional, Morabito, Giuseppe, additional, Degl'Innocenti, Andrea, additional, Giudetti, Guido, additional, Giannaccini, Martina, additional, and Andreazzoli, Massimiliano, additional
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- 2023
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21. Oleoylethanolamide decreases frustration stress-induced binge-like eating in female rats: a novel potential treatment for binge eating disorder
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Romano, Adele, Micioni Di Bonaventura, Maria Vittoria, Gallelli, Cristina Anna, Koczwara, Justyna Barbara, Smeets, Dorien, Giusepponi, Maria Elena, De Ceglia, Marialuisa, Friuli, Marzia, Micioni Di Bonaventura, Emanuela, Scuderi, Caterina, Vitalone, Annabella, Tramutola, Antonella, Altieri, Fabio, Lutz, Thomas A., Giudetti, Anna Maria, Cassano, Tommaso, Cifani, Carlo, and Gaetani, Silvana
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- 2020
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22. Oleic acid and olive oil polyphenols downregulate fatty acid and cholesterol synthesis in brain and liver cells
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Gnoni, Antonio, primary, Longo, Serena, additional, Damiano, Fabrizio, additional, Gnoni, Gabriele Vincenzo, additional, and Giudetti, Anna Maria, additional
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- 2021
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23. List of contributors
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Abia, Rocio, primary, Abouloifa, H., additional, Adriana, Morales-Martínez, additional, Aggarwal, Diwakar, additional, Aggarwal, Vaishali, additional, Alicia, Sánchez-Mendoza, additional, Andreadou, Ioanna, additional, Angeles-Lo´pez, Quetzalli D., additional, Argüelles, Sandro, additional, Asehraou, A., additional, Audi, Namaa, additional, Ayala, Antonio, additional, Ayoub, Nehad M., additional, Badiu, Diana, additional, Badria, Farid A., additional, Barbouti, Alexandra, additional, Batanero, Eva, additional, Batarseh, Yazan S., additional, Baumann, Leslie S., additional, Ben Salah, R., additional, Bermudez, Beatriz, additional, Blanco, Santos, additional, Borras-Linares, Isabel, additional, Brasca, M., additional, Brenes, Manuel, additional, Perez-Calabuig, Ana M., additional, Cancilla, John C., additional, Cano, Mercedes, additional, Carbone, Fabrizio, additional, Carrera-González, María Pilar, additional, Casas, Rosa, additional, Ceccanti, Mauro, additional, Chaldakov, George N., additional, Covas, María-Isabel, additional, Culeddu, Nicola, additional, Cumaoğlu, Ahmet, additional, Curiel, José Antonio, additional, Damiano, Fabrizio, additional, de Castro, Antonio, additional, de Felipe, Félix López, additional, de la Torre, Rafael, additional, de las Rivas, Blanca, additional, Deiana, Pierfrancesco, additional, Dekanski, Dragana, additional, Dettori, Sandro, additional, Di Stefano, Vita, additional, Domínguez-Vías, German, additional, Dore, Antonio, additional, D’hallewin, G., additional, El Sayed, Khalid A., additional, Elgazar, Abdullah A., additional, Emanuelli, Tatiana, additional, Ferraguti, Giampiero, additional, Filigheddu, Maria Rosaria, additional, Fiore, Marco, additional, Fitó, Montserrat, additional, Francisca, Pérez-Severiano, additional, Fujiwara, Yoko, additional, Galaris, Dimitrios, additional, García, Pedro, additional, Ghabbour, N., additional, Giudetti, Anna Maria, additional, Gnoni, Antonio, additional, Gnoni, Gabriele Vincenzo, additional, Goulas, Vlasios, additional, Greco, Antonio, additional, Guclu, Gamze, additional, Haghbeen, Kamahldin, additional, Handjani, Farhad, additional, Heydari, Mojtaba, additional, Hosseini Mazinani, Mehdi, additional, Ishikawa, Tomoko, additional, Iuliano, Luigi, additional, Joel, Jiménez-Gómez, additional, Joshi, Asavari, additional, Juan, M. Emília, additional, Jugreet, Bibi Sharmeen, additional, Kaddoumi, Amal, additional, Kailis, Stanley George, additional, Kanavaros, Panagiotis, additional, Karboune, S., additional, Kelebek, Hasim, additional, Kitsoulis, Panagiotis, additional, Kouka, Paraskevi, additional, Kouretas, Demetrios, additional, Ktari, N., additional, Kumar, Gaurav, additional, Kumar, Manoj, additional, Landete, José María, additional, Lauretti, Elisabetta, additional, Lemus-Conejo, Ana, additional, Lombardo, Luca, additional, Longo, Serena, additional, Pradana-Lopez, Sandra, additional, Lopez, Sergio, additional, Lopez-Millan, Belen, additional, López-López, Antonio, additional, Lozano-Sánchez, Jesús, additional, Madar, Zecharia, additional, Mahomoodally, Mohamad Fawzi, additional, Mañas, Mariano, additional, Martinez-Victoria, Emilio, additional, Martinez-Burgos, Maria Alba, additional, Martínez-Martos, José Manuel, additional, Masre, Siti Fathiah, additional, Medina, Eduardo, additional, Medina, Rafael, additional, Menendez, Javier A., additional, Millan-Linares, Maria C., additional, Mittal, Sonam, additional, Mohammadnejad, Parvin, additional, Molinu, Maria Giovanna, additional, Montaño, Alfredo, additional, Montserrat-de la Paz, Sergio, additional, Muñoz, Mario, additional, Muñoz, Rosario, additional, Muriana, Francisco J.G., additional, Nenadis, N., additional, Nychas, G.-J.E., additional, Ortega-García, Francisca, additional, Ortega-Gomez, Almudena, additional, Pacheco, Yolanda M., additional, Panagou, E.Z., additional, Papoti, V.T., additional, Pariente, Jose Antonio, additional, Parvizi, Mohammad Mahdi, additional, Pathak, Kaveri, additional, Peinado, M. Ángeles, additional, Peragón, Juan, additional, Petrella, Carla, additional, Čabarkapa-Pirkovic, Andrea, additional, Planas, Joana M., additional, Plastina, Pierluigi, additional, Praticò, Domenico, additional, Prieto, Isabel, additional, Rajendram, Rajkumar, additional, Ralli, Massimo, additional, Ramírez-Expósito, María Jesús, additional, Ramírez-Sánchez, Manuel, additional, Rasouli, Hassan, additional, Al Rihani, Sweilem B., additional, Rodríguez, Héctor, additional, Rodríguez-López, Paloma, additional, Rokni, Y., additional, Romero, Concepción, additional, Rosillo, Maria A., additional, Ruiz-Barba, José Luis, additional, Saalaoui, E., additional, Sacanella, Emilio, additional, Sadeer, Nabeelah Bibi, additional, Sak, Katrin, additional, Saki, Maryam, additional, Salimonti, Amelia, additional, Sánchez, Antonio-Higinio, additional, Santona, Mario, additional, Segarra, Ana Belén, additional, Segura-Carretero, Antonio, additional, Selli, Serkan, additional, Sergio, Montes, additional, Sethi, Gautam, additional, Seyedebrahimi, Seyede Sanaz, additional, Shahbaz, Mana, additional, Sharma, Dhvani, additional, Soares, Mario J, additional, Spremo-Potparević, Biljana, additional, Stark, Aliza Hannah, additional, Suroowan, Shanoo, additional, Suvarna, Vasanti, additional, Tahiri, Iasim, additional, Tarani, Luigi, additional, Tassou, C.C., additional, Topalović, Dijana, additional, Torrecilla, José S., additional, Tseti, Ioulia, additional, Tsimidou, M.Z., additional, Tsoumani, Maria, additional, Tuli, Hardeep Singh, additional, Varela, Lourdes M., additional, Veskoukis, Aristidis S., additional, Villalba, Mayte, additional, Weisberg, Edmund M., additional, Yago, Maria Dolores, additional, Yang, Euitaek, additional, Yerer, Mükerrem Betül, additional, Zanwar, Anand, additional, Zelasco, Samanta, additional, and Živković, Lada, additional
- Published
- 2021
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24. Urinary Metabolic Biomarkers in Cancer Patients: An Overview
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De Matteis, Serena, primary, Bonafè, Massimiliano, additional, and Giudetti, Anna Maria, additional
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- 2021
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25. M1-derived extracellular vesicles polarize recipient macrophages into M2 and alter skeletal muscle homeostasis in a hyper-glucose environment
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Tacconi, Stefano, primary, Vari, Francesco, additional, Sbarigia, Carolina, additional, Vardanyan, Diana, additional, Longo, Serena, additional, Mura, Francesco, additional, Angile, Federica, additional, Jalabart, Audrey, additional, Vergara, Daniele, additional, Fanizzi, Francesco Paolo, additional, Rossi, Marco, additional, Errazuriz-Cerda, Elizabeth, additional, Cassin, Christel, additional, Nieuwland, Rienk, additional, Giudetti, Anna Maria, additional, Rome, Sophie, additional, and Dini, Luciana, additional
- Published
- 2023
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26. Editorial: Lipid metabolism in obesity
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Giudetti, Anna Maria, primary
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- 2023
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27. Neural Responses of Benefiting From the Prosocial Exchange: The Effect of Helping Behavior
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Daniele Olivo, Andrea Di Ciano, Jessica Mauro, Lucia Giudetti, Alan Pampallona, Katharina M. Kubera, Dusan Hirjak, Robert Christian Wolf, and Fabio Sambataro
- Subjects
prosocial exchange ,practical help ,effort appreciation ,fMRI ,helping behavior ,theory of mind ,Psychology ,BF1-990 - Abstract
Prosocial behavior is critical for the natural development of an individual as well as for promoting social relationships. Although this complex behavior results from gratuitous acts occurring between an agent and a recipient and a wealth of literature on prosocial behavior has investigated these actions, little is known about the effects on the recipient and the neurobiology underlying them. In this study, we used functional magnetic resonance imaging to identify neural correlates of receiving prosocial behavior in the context of real-world experiences, with different types of action provided by the agent, including practical help and effort appreciation. Practical help was associated with increased activation in a network of regions spanning across bilateral superior temporal sulcus, temporoparietal junction, temporal pole, and medial prefrontal cortex. Effort appreciation was associated with activation and increased task-modulated connectivity of the occipital cortex. Prosocial-dependent brain responses were associated with positive affect. Our results support the role of the theory of mind network and the visual cortices in mediating the positive effects of receiving gratuitous help. Moreover, they indicate that specific types of prosocial behavior are mediated by distinct brain networks, which further demonstrates the uniqueness of the psychological processes underlying prosocial actions.
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- 2021
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28. Editorial: Dietary Antioxidants and Metabolic Diseases
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Daniele Vergara, Egeria Scoditti, Azlina Abdul Aziz, and Anna M. Giudetti
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dietary nutrients ,cardiometabolic diseases ,inflammation ,oxidative stress ,type 2 diabetes ,Nutrition. Foods and food supply ,TX341-641 - Published
- 2021
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29. Decanoic Acid and Not Octanoic Acid Stimulates Fatty Acid Synthesis in U87MG Glioblastoma Cells: A Metabolomics Study
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Fabrizio Damiano, Giuseppe E. De Benedetto, Serena Longo, Laura Giannotti, Daniela Fico, Luisa Siculella, and Anna M. Giudetti
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citric acid cycle ,decanoic acid ,lipid synthesis ,metabolomics ,octanoic acid ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Medium-chain fatty acids (MCFA) are dietary components with a chain length ranging from 6 to 12 carbon atoms. MCFA can cross the blood-brain barrier and in the brain can be oxidized through mitochondrial β-oxidation. As components of ketogenic diets, MCFA have demonstrated beneficial effects on different brain diseases, such as traumatic brain injury, Alzheimer’s disease, drug-resistant epilepsy, diabetes, and cancer. Despite the interest in MCFA effects, not much information is available about MCFA metabolism in the brain. In this study, with a gas chromatography-mass spectrometry (GC-MS)-based metabolomics approach, coupled with multivariate data analyses, we followed the metabolic changes of U87MG glioblastoma cells after the addition of octanoic (C8), or decanoic (C10) acids for 24 h. Our analysis highlighted significant differences in the metabolism of U87MG cells after the addition of C8 or C10 and identified several metabolites whose amount changed between the two groups of treated cells. Overall, metabolic pathway analyses suggested the citric acid cycle, Warburg effect, glutamine/glutamate metabolism, and ketone body metabolism as pathways influenced by C8 or C10 addition to U87MG cells. Our data demonstrated that, while C8 affected mitochondrial metabolism resulting in increased ketone body production, C10 mainly influenced cytosolic pathways by stimulating fatty acid synthesis. Moreover, glutamine might be the main substrate to support fatty acids synthesis in C10-treated cells. In conclusion, we identified a metabolic signature associated with C8 or C10 addition to U87MG cells that can be used to decipher metabolic responses of glioblastoma cells to MCFA treatment.
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- 2020
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30. Nutritional Regimes Enriched with Antioxidants as an Efficient Adjuvant for IBD Patients under Infliximab Administration, a Pilot Study
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Marina Liso, Annamaria Sila, Giulio Verna, Aurelia Scarano, Rossella Donghia, Fabio Castellana, Elisabetta Cavalcanti, Pasqua Letizia Pesole, Eduardo Maria Sommella, Antonio Lippolis, Raffaele Armentano, Anna Maria Giudetti, Daniele Vergara, Pietro Campiglia, Rodolfo Sardone, Margherita Curlo, Mauro Mastronardi, Katia Petroni, Chiara Tonelli, Angelo Santino, and Marcello Chieppa
- Subjects
antioxidant ,anthocyanins ,IBD ,diet ,infliximab ,pilot study ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Antioxidants are privileged candidates for the development of adjuvants able to improve the efficiency of pharmacological therapies, particularly for chronic inflammatory syndromes. During the last 20 years, anti-TNFα (tumor necrosis factor alpha) monoclonal antibodies infusion has been the biological therapy most frequently administered but there is still large space for improvement in disease remission rates and maintenance. In this context, nutritional bioactive compounds contained in dietary patterns or included as supplements, may act as adjuvants for the induction and maintenance of IBD (inflammatory bowel diseases) remission. To verify this possibility, a single-center preliminary study (SI-CURA, Soluzioni Innovative per la gestione del paziente e il follow up terapeutico della Colite UlceRosA) was designed and carried out to evaluate whether a daily administration of purple corn supplement could improve the response to Infliximab (IFX) infusion of IBD patients with both Crohn’s disease (CD) and ulcerative colitis (UC). A cohort of 47 patients was enrolled in the study. Biological samples were collected before the first and the third IFX infusion. All patients received nutritional guidelines, 27 of them received commercial red fruit tea with low anthocyanins content, while 20 received a purple corn supplement with a high anthocyanin content. Results show that the administration of an antioxidant-enriched purple corn supplement could improve IFX-mediated disease remission in terms of circulating inflammatory markers. Comparison between CD and UC patients revealed that, at this anthocyanin dosage, the purple corn extract administration improved the IFX response in CD but not in UC patients. Our results may pave the way for a new metacentric study of CD patients, recruiting a wider cohort and followed-up over a longer observational time.
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- 2022
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31. Modulatory effects of transcranial direct current stimulation of right insula on compassion motivation
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Di Bello, Maria, primary, Giudetti, Federica, additional, Palani, Sowmya, additional, Petrocchi, Nicola, additional, McIntosh, Roger, additional, and Ottaviani, Cristina, additional
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- 2023
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32. Multiscale Simulations of the Covalent Inhibition of the SARS-CoV-2 Main Protease: Four Compounds and Three Reaction Mechanisms
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Grigorenko, Bella L., primary, Polyakov, Igor V., additional, Khrenova, Maria G., additional, Giudetti, Goran, additional, Faraji, Shirin, additional, Krylov, Anna I., additional, and Nemukhin, Alexander V., additional
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- 2023
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33. Linking lipid peroxidation and neuropsychiatric disorders: focus on 4-hydroxy-2-nonenal
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Romano, Adele, Serviddio, Gaetano, Calcagnini, Silvio, Villani, Rosanna, Giudetti, Anna Maria, Cassano, Tommaso, and Gaetani, Silvana
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- 2017
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34. Anti-nuclear autoantibodies in Graves’ disease and Graves’ orbitopathy
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G. Lanzolla, L. Puccinelli, M. Giudetti, S. Comi, F. Menconi, M. N. Maglionico, C. Posarelli, M. Figus, C. Marcocci, and M. Marinò
- Subjects
Endocrinology ,Endocrinology, Diabetes and Metabolism - Abstract
Objective A relationship between thyroid and non-organ-specific autoimmunity could be relevant for Graves’ orbitopathy (GO), which affects connective tissue. We investigated the association between GO and anti-nuclear antibodies (ANAs). Methods Retrospective investigation was conducted in 265 patients with Graves’ disease (GD), 158 with and 107 without GO. Primary outcome was: prevalence of ANAs in GO vs no-GO. Secondary outcomes were: (1) relationship between ANAs and GO features; (2) prevalence of ANAs in GD compared with non-autoimmune hyperthyroidism [(78 patients with toxic nodular goiter (TNG)]; (3) distribution of ANA patterns. Results ANAs were detected in 212 (80%) GD patients, but prevalence did not differ between GO (79.7%) and no-GO (80.3%). Higher ANA titers (1:160) were more common in GO (51.5 vs 38.3%), but only nearly significantly (OR 0.5; 95% CI: 0.3–1; P = 0.059). Proptosis was lower in ANA-positive patients (mean difference: − 1.4 mm; 95% CI from − 2.5 to − 0.3; P = 0.011), in whom nearly significantly lower CAS (Mann–Whitney U: 1.5; P = 0.077) and eyelid aperture (mean difference: − 0.9 mm; 95% CI from − 2 to 0; P = 0.062) were observed. Prevalence of ANAs in GD was lower than in TNG (80 vs 91%; OR 0.3; 95% CI: 0.1–0.9; P = 0.028), but nuclear speckled pattern was more frequent (OR 22.9; 95% CI 1.3–381.3; P = 0.028). Conclusions Although ANAs are not more frequent in GO, they seem to exert a protective role on its severity and on development of GD. A switch of T cell population in ANA-positive patients, resulting in a different phenotype, may be responsible. Further studies are needed to investigate the mechanisms.
- Published
- 2022
35. Oxidative Stress and Multi-Organel Damage Induced by Two Novel Phytocannabinoids, CBDB and CBDP, in Breast Cancer Cells
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Maria Salbini, Alessandra Quarta, Fabiana Russo, Anna Maria Giudetti, Cinzia Citti, Giuseppe Cannazza, Giuseppe Gigli, Daniele Vergara, and Antonio Gaballo
- Subjects
MCF-7 ,cannabidiol ,ROS ,oxidative stress ,autophagy ,altered mitochondria ,Organic chemistry ,QD241-441 - Abstract
Over the last few years, much attention has been paid to phytocannabinoids derived from Cannabis for their therapeutic potential. Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the most abundant compounds of the Cannabis sativa L. plant. Recently, novel phytocannabinoids, such as cannabidibutol (CBDB) and cannabidiphorol (CBDP), have been discovered. These new molecules exhibit the same terpenophenolic core of CBD and differ only for the length of the alkyl side chain. Roles of CBD homologs in physiological and pathological processes are emerging but the exact molecular mechanisms remain to be fully elucidated. Here, we investigated the biological effects of the newly discovered CBDB or CBDP, compared to the well-known natural and synthetic CBD (nat CBD and syn CBD) in human breast carcinoma cells that express CB receptors. In detail, our data demonstrated that the treatment of cells with the novel phytocannabinoids affects cell viability, increases the production of reactive oxygen species (ROS) and activates cellular pathways related to ROS signaling, as already demonstrated for natural CBD. Moreover, we observed that the biological activity is significantly increased upon combining CBD homologs with drugs that inhibit the activity of enzymes involved in the metabolism of endocannabinoids, such as the monoacylglycerol lipase (MAGL) inhibitor, or with drugs that induces the activation of cellular stress pathways, such as the phorbol ester 12-myristate 13-acetate (PMA).
- Published
- 2021
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36. Oleoylethanolamide Reduces Hepatic Oxidative Stress and Endoplasmic Reticulum Stress in High-Fat Diet-Fed Rats
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Anna Maria Giudetti, Daniele Vergara, Serena Longo, Marzia Friuli, Barbara Eramo, Stefano Tacconi, Marco Fidaleo, Luciana Dini, Adele Romano, and Silvana Gaetani
- Subjects
oleoylethanolamide ,nuclear factor erythroid-derived 2-related factor 1 (Nrf1) ,nuclear factor erythroid-derived 2-related factor 2 (Nrf2) ,oxidative stress ,diet-induced obesity ,non-alcoholic fatty liver ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Long-term high-fat diet (HFD) consumption can cause weight gain and obesity, two conditions often associated with hepatic non-alcoholic fatty liver and oxidative stress. Oleoylethanolamide (OEA), a lipid compound produced by the intestine from oleic acid, has been associated with different beneficial effects in diet-induced obesity and hepatic steatosis. However, the role of OEA on hepatic oxidative stress has not been fully elucidated. In this study, we used a model of diet-induced obesity to study the possible antioxidant effect of OEA in the liver. In this model rats with free access to an HFD for 77 days developed obesity, steatosis, and hepatic oxidative stress, as compared to rats consuming a low-fat diet for the same period. Several parameters associated with oxidative stress were then measured after two weeks of OEA administration to diet-induced obese rats. We showed that OEA reduced, compared to HFD-fed rats, obesity, steatosis, and the plasma level of triacylglycerols and transaminases. Moreover, OEA decreased the amount of malondialdehyde and carbonylated proteins and restored the activity of antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, which decreased in the liver of HFD-fed rats. OEA had also an improving effect on parameters linked to endoplasmic reticulum stress, thus demonstrating a role in the homeostatic control of protein folding. Finally, we reported that OEA differently regulated the expression of two transcription factors involved in the control of lipid metabolism and antioxidant genes, namely nuclear factor erythroid-derived 2-related factor 1 (Nrf1) and Nrf2, thus suggesting, for the first time, new targets of the protective effect of OEA in the liver.
- Published
- 2021
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37. QM/MM Simulations of the Covalent Inhibition of the SARS-CoV-2 Main Protease: Four Compounds and Three Reaction Mechanisms
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Bella Grigorenko, Igor Polyakov, Maria Khrenova, Goran Giudetti, Shirin Faraji, Anna Krylov, and Alexander Nemukhin
- Abstract
These files are required to reproduce the analysis of the components of the collective variable CV2=d(NE-HS)-d(HS-OWat1)+d(OWat1-HWat1)-d(HWat1-OWat2)+d(OWat2-HWat2)-d(N-HWat2) used for biased umbrella sampling calculations of the 2nd stage of the reaction between nirmatrelvir and SARS-CoV-2 main protease. Extract the 7z archive to obtain the 'traj' directory with all the individual DCD trajectory files
- Published
- 2023
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38. Tracing Ancient Carbon Dioxide Emission in the Larderello Area by Means of Historical Boric Acid Production Data
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Alessandro Lenzi, Marco Paci, Geoffrey Giudetti, and Roberto Gambini
- Subjects
sustainability ,geothermal resources ,CO2 emission ,soil emission ,boric acid ,lagone ,Technology - Abstract
The impact of natural CO2 emissions in the development of geothermal areas is presently gaining more attention than ever before. In Italian geothermal fields, a reduction in the natural CO2 emissions has been observed. This paper reviews and provides an analysis of the historical production data of boric acid from 1818 to 1867 used to calculate the natural emissions of CO2 associated with boric acid production that pre-dates the use of geothermal resources for power production, which started in 1913. Boric acid was already being extracted from the natural geothermal fluids in geysers and natural ponds emitting steam and gases. After 1827 the ‘lagone coperto’ (covered lake) equipment optimized production, and the drilling of shallow wells (20–30 m) starting in 1836, which further increased the quantity of its extraction. The first geothermal reservoir was developed at the turn of the century and the Larderello geothermal field began to grow. The use of deep wells, keeping pace with the power production, led to the gradual disappearance of the natural ponds and the ‘lagoni’ (lakes) in the historical area, so the residual natural emission of CO2 is presently restricted to diffuse soil emission. Comparisons of the ancient CO2 emissions with those of the Geothermal Power Plant (GPP) in the Larderello area show that both amounts are in the same order of magnitude, suggesting a balance between the depletion of natural emissions and geothermal activity.
- Published
- 2021
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39. Effects of a Diet Based on Foods from Symbiotic Agriculture on the Gut Microbiota of Subjects at Risk for Metabolic Syndrome
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Silvia Turroni, Elisabetta Petracci, Valeria Edefonti, Anna M. Giudetti, Federica D’Amico, Lisa Paganelli, Giusto Giovannetti, Laura Del Coco, Francesco P. Fanizzi, Simone Rampelli, Debora Guerra, Claudia Rengucci, Jenny Bulgarelli, Marcella Tazzari, Nicoletta Pellegrini, Monica Ferraroni, Oriana Nanni, and Patrizia Serra
- Subjects
adult volunteers ,dietary intervention ,dietary patterns ,gut microbiota ,pilot study ,symbiotic agriculture ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Diet is a major driver of gut microbiota variation and plays a role in metabolic disorders, including metabolic syndrome (MS). Mycorrhized foods from symbiotic agriculture (SA) exhibit improved nutritional properties, but potential benefits have never been investigated in humans. We conducted a pilot interventional study on 60 adults with ≥ 1 risk factors for MS, of whom 33 consumed SA-derived fresh foods and 27 received probiotics over 30 days, with a 15-day follow-up. Stool, urine and blood were collected over time to explore changes in gut microbiota, metabolome, and biochemical, inflammatory and immunologic parameters; previous dietary habits were investigated through a validated food-frequency questionnaire. The baseline microbiota showed alterations typical of metabolic disorders, mainly an increase in Coriobacteriaceae and a decrease in health-associated taxa, which were partly reversed after the SA-based diet. Improvements were observed in metabolome, MS presence (two out of six subjects no longer had MS) or components. Changes were more pronounced with less healthy baseline diets. Probiotics had a marginal, not entirely favorable, effect, although one out of three subjects no longer suffered from MS. These findings suggest that improved dietary patterns can modulate the host microbiota and metabolome, counteracting the risk of developing MS.
- Published
- 2021
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40. Increased intake of energy-dense diet and negative energy balance in a mouse model of chronic psychosocial defeat
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Coccurello, Roberto, Romano, Adele, Giacovazzo, Giacomo, Tempesta, Bianca, Fiore, Marco, Giudetti, Anna Maria, Marrocco, Ilaria, Altieri, Fabio, Moles, Anna, and Gaetani, Silvana
- Published
- 2018
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41. Neural Correlates of Antisocial Behavior: The Victim’s Perspective
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Trevisan, Nicolò, primary, Cattarinussi, Giulia, additional, Olivo, Daniele, additional, Di Ciano, Andrea, additional, Giudetti, Lucia, additional, Pampallona, Alan, additional, Kubera, Katharina M., additional, Hirjak, Dusan, additional, Wolf, Robert Christian, additional, and Sambataro, Fabio, additional
- Published
- 2023
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- View/download PDF
42. QM/MM Simulations of the Covalent Inhibition of the SARS-CoV-2 Main Protease: Four Compounds and Three Reaction Mechanisms
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Grigorenko, Bella L., primary, Polyakov, Igor V., additional, Khrenova, Maria G., additional, Giudetti, Goran, additional, Faraji, Shirin, additional, Krylov, Anna I., additional, and Nemukhin, Alexander V., additional
- Published
- 2023
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43. Origin of Magnetic Anisotropy in Nickelocene Molecular Magnet and Resilience of Its Magnetic Behavior
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Alessio, Maristella, primary, Kotaru, Saikiran, additional, Giudetti, Goran, additional, and Krylov, Anna I., additional
- Published
- 2023
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44. Modulation of the Circulating Extracellular Vesicles in Response to Different Exercise Regimens and Study of Their Inflammatory Effects
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Maggio, Serena, primary, Canonico, Barbara, additional, Ceccaroli, Paola, additional, Polidori, Emanuela, additional, Cioccoloni, Andrea, additional, Giacomelli, Luca, additional, Ferri Marini, Carlo, additional, Annibalini, Giosuè, additional, Gervasi, Marco, additional, Benelli, Piero, additional, Fabbri, Francesco, additional, Del Coco, Laura, additional, Fanizzi, Francesco Paolo, additional, Giudetti, Anna Maria, additional, Lucertini, Francesco, additional, and Guescini, Michele, additional
- Published
- 2023
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- View/download PDF
45. Translating epithelial mesenchymal transition markers into the clinic: Novel insights from proteomics
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Vergara, Daniele, Simeone, Pasquale, Franck, Julien, Trerotola, Marco, Giudetti, Anna, Capobianco, Loredana, Tinelli, Andrea, Bellomo, Claudia, Fournier, Isabelle, Gaballo, Antonio, Alberti, Saverio, Salzet, Michel, and Maffia, Michele
- Published
- 2016
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46. QM/MM Simulations of the Covalent Inhibition of the SARS-CoV-2 Main Protease: Four Compounds and Three Reaction Mechanisms
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Bella L. Grigorenko, Igor V. Polyakov, Maria G. Khrenova, Goran Giudetti, Shirin Faraji, Anna I. Krylov, and Alexander V. Nemukhin
- Abstract
The search for efficient inhibitors of the SARS-CoV-2 enzymes is ongoing due to the continuing COVID-19 pandemic. We report the results of computational modeling of the reactions of the SARS-CoV-2 main protease (MPro ) with four potential covalent inhibitors. Two of them, carmofur and nirmatrelvir, have been shown experimentally the ability to inhibit MPro . Two other compounds, X77A and X77C, were designed computationally in this work, derived from the structure of X77, a non-covalent inhibitor forming a tight surface complex with MPro . We modified the X77 structure by introducing warheads capable of efficient chemical reactions with the catalytic cysteine residue in the M Pro active site. The reaction mechanisms of the four molecules with M Pro were investigated by quantum mechanics/molecular mechanics (QM/MM) calculations using large quantum subsystems. First, at the QM/MM level, we optimized structures of stationary points on the potential energy surfaces corresponding to the reactants, products, intermediates, and transition states along the hypothesized reaction coordinates. Analysis of these structures has informed the selection of collective variables for the subsequent calculations of the Gibbs energy profiles using molecular dynamics simulations with QM/MM potentials (QM/MM MD). In these simulations, the QM part was treated by DFT with the PBE0 functional. The results show that all four compounds form covalent adducts with the catalytic cysteine Cys 145 of MPro . From the chemical perspective, the reactions of these four compounds with M Pro follow three distinct mechanisms. In all cases, the reaction is initiated by a nucleophilic attack of the thiolate group of the deprotonated cysteine residue from the catalytic dyad Cys145-His41 of MPro . In the case of carmofur and X77A, the covalent binding of the thiolate to the ligand is accompanied by the formation of the fluoro-uracil leaving group. The reaction with X77C follows the nucleophilic aromatic substitution SN Ar mechanism. The reaction of M Pro with nirmatrelvir, which has a reactive nitrile group, leads to the formation of the covalent thioimidate adduct with the thiolate of the Cys145 residue in the enzyme active site.
- Published
- 2023
47. Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPARγ/SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis
- Author
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Adele Romano, Marzia Friuli, Laura Del Coco, Serena Longo, Daniele Vergara, Piero Del Boccio, Silvia Valentinuzzi, Ilaria Cicalini, Francesco P. Fanizzi, Silvana Gaetani, and Anna M. Giudetti
- Subjects
lipid metabolism ,oleoylethanolamide ,peroxisome proliferator-activated receptorγ ,NMR spectroscopy ,sphingolipids ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Oleoylethanolamide (OEA) is a naturally occurring bioactive lipid belonging to the family of N-acylethanolamides. A variety of beneficial effects have been attributed to OEA, although the greater interest is due to its potential role in the treatment of obesity, fatty liver, and eating-related disorders. To better clarify the mechanism of the antiadipogenic effect of OEA in the liver, using a lipidomic study performed by 1H-NMR, LC-MS/MS and thin-layer chromatography analyses we evaluated the whole lipid composition of rat liver, following a two-week daily treatment of OEA (10 mg kg−1 i.p.). We found that OEA induced a significant reduction in hepatic triacylglycerol (TAG) content and significant changes in sphingolipid composition and ceramidase activity. We associated the antiadipogenic effect of OEA to decreased activity and expression of key enzymes involved in fatty acid and TAG syntheses, such as acetyl-CoA carboxylase, fatty acid synthase, diacylglycerol acyltransferase, and stearoyl-CoA desaturase 1. Moreover, we found that both SREBP-1 and PPARγ protein expression were significantly reduced in the liver of OEA-treated rats. Our findings add significant and important insights into the molecular mechanism of OEA on hepatic adipogenesis, and suggest a possible link between the OEA-induced changes in sphingolipid metabolism and suppression of hepatic TAG level.
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- 2021
- Full Text
- View/download PDF
48. Nutritional Status and Cancer: Early Detection of Malnutrition and Sarcopenia in Elderly Patients at High Risk for Cachexia by Using a Multi-Score Protocol
- Author
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Guarini, Vincenzo, primary, Zurlo, Ina Valeria, additional, Giudetti, Anna Maria, additional, Leo, Chiara, additional, Leone, Stefania, additional, Piscitelli, Prisco, additional, Saracino, Valeria, additional, and Leo, Silvana, additional
- Published
- 2023
- Full Text
- View/download PDF
49. A bio-hybrid mechanotransduction system based on ciliate cells
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Salgarella, Alice Rita, Giudetti, Guido, Ricotti, Leonardo, Camboni, Domenico, Puleo, Gian Luigi, Ruini, Francesca, Tonda-Turo, Chiara, Chiono, Valeria, Ciardelli, Gianluca, Micera, Silvestro, Menciassi, Arianna, and Oddo, Calogero Maria
- Published
- 2015
- Full Text
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50. Bioenergetics profile of CD4+ T cells in relapsing remitting multiple sclerosis subjects
- Author
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De Riccardis, Lidia, Rizzello, Antonia, Ferramosca, Alessandra, Urso, Emanuela, De Robertis, Francesca, Danieli, Antonio, Giudetti, Anna Maria, Trianni, Giorgio, Zara, Vincenzo, and Maffia, Michele
- Published
- 2015
- Full Text
- View/download PDF
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