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These work was carried out in the Plant Protection Laboratory of the Agronomy Faculty in the Agrarian University of La Habana, to determinate the in vitro effectivity of the isolate Ma-005 of Metarhizium anisopliae s.l (Metsch.) Sorokin on eggs and neolarvae of Rhipicephalus microplus Canestrini. Eggs, with five to seven days old, and neolarvae with seven to 10 days old, were inoculated by immersion during one minute in suspensions with different conidial concentration of the isolate. It was evaluated the hatching of larvae from eggs after 30 days and neolarval mortality after seven and 14 days. It was observed a high effect of the conidia concentration on hatching of larvae from eggs, less than 10 % at the highest of the concentrations evaluated, and the mortality of the tick neolarvae, more than 80 % at the concentration of 108 conidia mL-1.