1. In vitro test system for compounds affecting cholesterol pathway: Studies in primary rat liver cell cultures
- Author
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Karlheinz Stegmeier, Albrecht Frey, Johannes Pill, Gisela Johne, Johannes Dr Aufenanger, and Barbara Frey
- Subjects
Silica gel ,Cholesterol ,Clinical Biochemistry ,Extraction (chemistry) ,Biomedical Engineering ,Bioengineering ,Cell Biology ,Biology ,In vitro ,Silver nitrate ,chemistry.chemical_compound ,Biochemistry ,chemistry ,In vivo ,Cell culture ,Xenobiotic ,Biotechnology - Abstract
Rat liver cells derived from male and female animals in primary monolayer cultures were investigated for suitability as a test system for xenobiotics affecting the cholesterol pathway. An appropriate mode of extraction and separation of newly formed cholesterol and precursors is described. This system can be widely applied. Rat liver cells from females in oestrus cycle had a higher synthesis rate of cholesterol than those from males. The disadvantages related to the cycle phases make male rats more appropriate donor animals for the test system developed. The altered in vitro cholesterol synthesis is relevant to that in vivo. The extraction of newly synthesized cholesterol and its precursors by means of columns packed with large-pore kieselgur is precise and time saving. The modified separation by thin-layer chromatography on silica gel layers impregnated with silver nitrate enables direct separation from the extract and is sufficient to recognize cholesterol and its precursors. The method in this form is suitable for processing a large number of specimens e.g. for screening. more...
- Published
- 1987