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Microplastics (MPs) pose a clear threat to aquatic organisms affecting their health. Their impact on liver homeostasis, as well as on the potential onset of nonalcoholic fatty liver disease (NAFLD), is still poorly investigated and remains almost unknown. The aim of this study was to evaluate the outcomes of subchronic exposure to polystyrene MPs (PS-MPs; 1–20 μm; 0, 25, or 250 mg/kg b.w./day) on lipid metabolism, inflammation, and oxidative balance in the liver of gilthead seabreams (Sparus aurata Linnaeus, 1758) exposed for 21 days via contaminated food. PS-MPs induced an up-regulation of mRNA levels of crucial genes associated with lipid synthesis and storage (i.e., PPARy, Srebp1, Fasn) without modifications of genes involved in lipid catabolism (i.e., PPARα, HL, Pla2) or transport and metabolism (Fabp1) in the liver. The increase of CSF1R and pro-inflammatory cytokines gene expression (i.e., TNF-α and IL-1β) was also observed in exposed fish in a dose-dependent manner. These findings were confirmed by hepatic histological evaluations reporting evidence of lipid accumulation, inflammation, and necrosis. Moreover, PS-MPs caused the impairment of the hepatic antioxidant defense system through the alteration of its enzymatic (catalase, superoxide dismutase, and glutathione reductase) and non-enzymatic (glutathione) components, resulting in the increased production of reactive oxygen species (ROS) and malondialdehyde (MDA), as biomarkers of oxidative damage. The alteration of detoxifying enzymes was inferred by the decreased Ethoxyresorufin-O-deethylase (EROD) activity and the increased activity of glutathione-S-transferase (GST) at the highest PS-MP dose. The study suggests that PS-MPs affect the liver health of gilthead seabream. The liver dysfunction and damage caused by exposure to PS-MPs result from a detrimental interplay of inflammation, oxidative damage, and antioxidant and detoxifying enzymatic systems modifications, altering the gut-liver axis homeostasis. This scenario is suggestive of the involvement of MP-induced effects in the onset and progression of hepatic lipid dysfunction in gilthead seabream.

Abstract Herein, the effect of dietary inclusion of insect (Tenebrio molitor) meal on hepatic pathways of apoptosis and autophagy in three farmed fish species, gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss), fed diets at 25%, 50% and 60% insect meal inclusion levels respectively, was investigated. Hepatic proteome was examined by liver protein profiles from the three fish species, obtained by two-dimensional gel electrophoresis. Although cellular stress was evident in the three teleost species following insect meal, inclusion by T. molitor, D. labrax and O. mykiss suppressed apoptosis through induction of hepatic autophagy, while in S. aurata both cellular procedures were activated. Protein abundance showed that a total of 30, 81 and 74 spots were altered significantly in seabream, European seabass and rainbow trout, respectively. Insect meal inclusion resulted in individual protein abundance changes, with less number of proteins altered in gilthead seabream compared to European seabass and rainbow trout. This is the first study demonstrating that insect meal in fish diets is causing changes in liver protein abundances. However, a species-specific response both in the above mentioned bioindicators, indicates the need to strategically manage fish meal replacement in fish diets per species.

Microplastics (MPs) are pollutants widely distributed in aquatic ecosystems. MPs are introduced mainly by ingestion acting locally or in organs far from the gastroenteric tract. MPs-induced health consequences for fish species still need to be fully understood. We aimed to investigate the effects of the subchronic oral exposure to polystyrene microplastics (PS-MPs) (1–20 μm) in the gilthead seabreams (Sparus aurata) used as the experimental model. We studied the detrimental impact of PS-MPs (25 and 250 mg/kg b.w./day) on the redox balance and antioxidant status in the intestine using histological analysis and molecular techniques. The research goal was to examine the anterior (AI) and posterior intestine (PI) tracts, characterized by morphological and functional differences. PS-MPs caused an increase of reactive oxygen species and nitrosylated proteins in both tracts, as well as augmented malondialdehyde production in the PI. PS-MPs also differently affected gene expression of antioxidant enzymes (i.e., superoxide dismutase, catalase, glutathione reductase). Moreover, an increased up-regulation of protective heat shock proteins (HSPs) (i.e., hsp70 and hsp90) was observed in PI. Our findings demonstrate that PS-MPs are responsible for oxidative/nitrosative stress and alterations of detoxifying defense system responses with differences in AI and PI of gilthead seabreams.

This paper reviews current knowledge on two feedstuffs, that is, insect meal and fish by-products, as alternatives to conventional animal protein sources. After an introductory part that highlights the need for sustainable development of animal production, the alternative protein sources are discussed. In particular, after providing some indications on their production and supply focussing on EU, a SWOT (Strengths, Weaknesses, Opportunities, and Threats) analysis was performed to identify the key factors that could help or impair the development of both protein sources production sectors. Finally, future perspectives are presented. The use of processed animal proteins derived from insects in farmed fish feeding is recognised by the EU legislation that authorises the use of proteins from seven insect species and the allowed substrates to rear insects. Insects have several advantages in nutritional value and the amino acid composition of their proteins generally meet animal requirements for good growth and health. The SWOT analysis indicated that insect meals can be considered as feed functional ingredients with beneficial properties that depend on the insect species, rearing system adopted, and the substrate used for their growth. Insects are expected to be increasingly used as a replacement for conventional animal-derived proteins, especially in aquafeeds. In the section regarding fishery and aquaculture by-products, the potential use of raw materials obtained during seafood processing is discussed. Peptides and amino acids recovered from as hydrolysed proteins can be used in animal feeds to partially substitute conventional protein feedstuffs thus providing nutrients, bioactive compounds and feed additives for animals. The SWOT analysis identified opportunities and weaknesses. Both the alternative protein sources are promising alternative feed ingredients for livestock production.Highlights The sustainable development of animal production sector needs alternative protein sources for feeds formulation. Insects and fishery- and aquaculture by-products represent optimal alternative protein sources. A SWOT analysis has identified the key factors for the development of both protein sources production sectors.

The expected future demand for highly nutrient animal food products will push the animal production system to search for new sources of high-quality protein feedstuffs. In this scenario, economic and environmental issues will have to be considered while reducing the competition with the plant-based human food chains. Legume grains and some oilseed cakes, by-products from the oil industry, are the main protein sources for ruminants and terrestrial monogastrics such as pigs and poultry. Their relevant role will hold in the next decades, but it is necessary to increase the diversification of sources that can be grown profitably throughout the world, including European countries. Microalgae are a promising source of protein and other nutrients for animal feeding. However, an amazing richness of biologically active substances makes these organisms very interesting as feed ingredients, as their role go far beyond the supply of nutrients. Due to the limited usage of microalgae as human foodstuffs or food ingredients, low competition between microalgae-based feed and food chains is predictable. This review aims to synthesise current knowledge on minor pulses and other protein-rich plant products and microalgae, as alternative ingredients to the conventional animal protein sources, focussing on their production, availability, and nutritional values. Points of strength, weakness, opportunity and threat related to the use of these protein sources in animal feeding are separately analysed through a SWOT approach to underlie future needs in terms of research and/or technological development that could help valorise these nutrient sources as feed ingredients.

The present study addresses the effects of dietary Tenebrio molitor (TM) larvae meal inclusion on cytoprotective, cell death pathways, antioxidant defence, and intermediate metabolism in the heart, muscle, and digestive tract of gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax). Three experimental diets were formulated to contain 0%, 25%, or 50% inclusion TM levels. Heat Shock Proteins (HSPs) induction was apparent in both species’ muscle at 50% inclusion. Conversely, p44/42 Mitogen-Activated Protein Kinase (MAPK) activation was increased (p

Volatile fatty acid production (VFA) in the caeca and fatty acid profile of meat and fat depots were investigated on 62 days old broiler equally divided in 2 groups fed with a corn-soybean (SBM) or a corn-insect meal (TML, from Tenebrio molitor larvae) based diet from 30 days of age. The total amount of VFAs was higher (p

The awareness of the correlation between administered diet, fish health and products’ quality has led to the increase in the research for innovative and functional feed ingredients. Herein, a plant-derived product rich in bioactive compounds, such as honeybee pollen (HBP), was included as raw (HBP) and as Supercritical Fluid Extracted (SFE) pollen (HBP_SFE) in the diet for gilthead seabream (Sparus aurata). The experiment was carried out on 90 fish with an average body weight of 294.7 ± 12.8 g, divided into five groups, according to the administration of five diets for 30 days: control diet (CTR); two diets containing HBP at 5% (P5) and at 10% (P10) level of inclusion; two diets containing HBP_SFE, at 0.5% (E0.5) and at 1% (E1) level of inclusion. Their effects were evaluated on 60 specimens (336.2 ± 11.4 g average final body weight) considering the fish growth, the expression of some hepatic genes involved in the inflammatory response (il-1β, il-6 and il-8) through quantitative real-time PCR, and physico-chemical characterization (namely color, texture, water holding capacity, fatty acid profile and lipid peroxidation) of the fish fillets monitored at the beginning (day 0) and after 110 days of storage at −20 °C. The results obtained showed that the treatment with diet E1 determined the up-regulation of il-1β, il-6, and il-8 (p < 0.05); however, this supplementation did not significantly contribute to limiting the oxidative stress. Nevertheless, no detrimental effect on color and the other physical characteristics was observed. These results suggest that a low level of HBP_SFE could be potentially utilized in aquaculture as an immunostimulant more than an antioxidant, but further investigation is necessary.

In the present study, the immune-stimulatory effect of two levels of honey bee pollen (5 and 10%, P5 and P10 treatment, respectively) and its supercritical fluid extract (0. 5 and 1%, E0.5 and E1, respectively) included in the diet, was tested in gilthead seabream (Sparus aurata). The in vivo trial was preceded by the evaluation of antioxidant properties of three different bee pollen extracts obtained by water, ethanol 80%, and Supercritic Fluids Extraction (SFE). The preliminary evaluation attested that the SFE showed the lowest extraction yield (10.47%) compared to ethanol 80% (48.61%) and water (45.99%). SFE extract showed good antioxidant properties with high polyphenol content (13.06 mg GAE/g), radical scavenging activity (3.12 mg/ml), reducing power (38.68 mg/mL EC50). On the contrary, the water extract showed the significantly lowest polyphenol content (2 mg GAE/g; P < 0.05). The results of in vivo trial demonstrate that the diets supplemented with SFE bee pollen extract had a stimulatory effect on fish serum immunity, respect to the inclusion of raw pollen, this latter revealing some inhibitory effects in the immune response, such a decrease of serum peroxidase and lysozyme activities, particularly in P10 group significantly different (P < 0.05) from the control group. On the contrary, serum peroxidase, protease, antiprotease, were significantly increased in fish fed the diets supplemented with supercritical fluid extract, respect to the fish fed on control and on diets supplemented with 5 and 10% of raw pollen. For what concerns the bactericidal activity against Vibrio harveyii, all the treatments containing bee pollen regardless of the type showed their serum bactericidal activity significantly increased with respect to the control groups (p < 0.05). Given its high antioxidant properties, the absence of toxic solvents and the positive action carried out on improving the humoral response in gilthead seam bream, honey bee pollen SFE extract can be taken into account in the formulation of fish feeds.

Hermetia illucens larvae (five days old) were farmed on broiler feed (control diet), a vegetable diet (V100), a 50% of vegetable diet + 50% of butchery wastes (V50 + B50), and a 75% of vegetable diet + 25% of butchery wastes (V75 + B25) to evaluate their suitability. Ten kilograms of substrate and 6000 larvae composed each replicate (nine per group). Larvae were weighed and measured every two days until the 25% developed into prepupae. Larval mortality and growing indexes were calculated. Substrates, larvae, and frass chemical composition were analyzed. Larvae oxidative status and stability were measured in hemolymph and body. The V100 larvae showed the lowest live weight, length, thickness, and growth rate but had low mortality rate and high substrate reduction index and protein conversion ratio. The V100 larvae had similar protein to and lower lipids than the control ones, while the V50 + B50 and V75 + B25 larvae contained higher lipids and lower protein than the others. Despite the vegetable wastes, at different levels, the reactive oxygen species content decreased in hemolymph, and the V100 diet depressed growth performance and should be avoided. The use of butchery wastes combined with vegetable ingredients can be a suitable alternative to balance the high level of lipid and the low content of protein.

This research aimed to evaluate the effects of the inclusion of honey bee pollen (HBP) in meagre (Argyrosoumus regius) juveniles’ diets on growth performance, diet digestibility, intestinal morphology, and immunohistochemistry. Furthermore, stress-related molecular markers and biochemical blood profile of fish were evaluated, together with mineral trace and toxic element concentration in pollen and diets. Specimens of meagre (360) of 3.34 ± 0.14 g initial body weight, were randomly allocated to twelve 500 L circular tanks (30 fish per tank). Four diets were formulated: a control diet and three experimental diets with 1%, 2.5%, and 4% of HBP inclusion. All the growth parameters and crude protein and ether extract digestibility coefficients were negatively linearly affected by increased HBP inclusion (p < 0.0001). Histology of medium intestine showed slight signs of alterations in group HPB1 and HPB2.5 compared to control. Fish from HBP4 group showed severe alterations at the intestinal mucosa level. Immunohistochemical detection of TNF-α in the medium intestine showed the presence of TNF-α+ cells in the lamina propria, which resulted in accordance with the increased level of the TNF-α protein detected by immunoblotting in the liver. This stress situation was confirmed by the increased hepatic level of HSP70 (p < 0.05) in fish fed the HBP4 diet and by the linear decrease of total serum protein levels in HBP-containing diets (p < 0.0001). These negative effects can be related to the ultrastructure of the bee pollen grain walls, which make the bioactive substances unavailable and can irritate the intestine of a carnivorous fish such as meagre.

The aim of this work was to propose a model of free-range raising for rabbit able to maximize the animal welfare and at the same time the productive performances through the use of mirrors. A total of 81 rabbits were allocated into free-range areas and divided into three groups (nine replicates per group): in the first group (face to face, F2F), the rabbits of each replicate could see each other. In the second group (blind) each replicate was isolated from the others; in the third group (mirrors), the replicates were divided as for the Blind group but two mirrors were placed in a corner of the perimeter. The blind group rabbits showed the lowest final weight (p < 0.05), while rabbits from the mirrors groups showed the best FCR and net dressing out values. The blind group showed the highest production of total short chain fatty acids, acetate (p < 0.05) and propionate (p < 0.01). The F2F rabbits showed higher levels of creatine phosphokinase and lactate dehydrogenase and lower values of blood glucose than those of the other groups, due to the higher locomotion activity. The use of mirrors can improve rabbit’s growth performance and carcass traits by lowering the rabbit’s locomotion activity in comparison to the other tested systems.

The aim of this research was to evaluate the possible usefulness of mirrors in improving rabbit behavior in a free-range breeding system. Three groups (each consisting of nine replicates of three animals) were compared: isolated, isolated with mirrors and separated by a wire mesh (possible visual and olfactory contacts). Rabbits allowed to have a visual and olfactory contact showed a significantly higher expression of important natural behaviors (olfactory investigation, gnawing, alertness, stretching, locomotion) compared to the isolated rabbits (with or without mirrors); while rabbits in the mirror group showed higher allo-grooming activity than those isolated and no different locomotion activity than those separated by wire mesh. Thus, mirrors seemed to be able to modify the behavioral repertoire of isolated rabbits by acting on social perception in rabbits reared in small groups in a free-range system. Nevertheless, despite it being advisable to use a combination of different indicators in order to assess the stress level of an animal, the accuracy of serum cortisol, as well as of some secondary stress markers assay, appeared to be limited in this type of breeding.

The aim of this study was to evaluate the effect of two diets with different protein/fat ratios (P/F) (diet A: P/F 2.26; diet B: P/F 3.36) on the chemical composition, fatty acid profile and some somatic indexes of meagre (Argyrosomus regius). The trial was carried out on two groups of meagre raised in two different sea cages during 15 months. At the end of the production cycle biometric measures as well as chemical-nutritional analysis of the fillets were conducted on 25 fishes per group. Diet A, with a lower P/F, furnished animals with higher percentages of mesenteric fat (0.48 vs 0.41%; P

The aim of the paper was to study the fermentation of the neutral detergent soluble fraction (NDS) using a 2x3x2 factorial arrangement of two intercropped forages (barley/broad bean and vetch/oats) collected at three growth stages and preserved by haymaking. Each feed sample and its isolated NDF were fermented in vitroand gas production was monitored utilizing the in vitrogas production technique (IVGPT). NDS gas yield was determined by the difference between gas from the unfractioned forage and that of its respective NDF. Haymaking decreased gas yield and rate of gas production from the unfractioned forage. Increasing maturi- ty did not change the final gas volume, but reduced the rate of gas production from the NDS fraction. The rate of gas production from the NDS fraction of hay decreased on average by 2.9 ml/h compared with fresh forage. On average, haymaking reduced the gas yield from the NDS fraction by 18.70%. The curve subtrac- tion technique can be used to study the effects of haymaking on the neutral detergent soluble fraction of for- ages.

In order to enhance our knowledge of milk sheep rationing, 30 Sarda primiparous ewes, for which single birth was diagnosedby ultrasonography prior to lambing, were divided into two homogeneous groups (L-NSC and H-NSC) and fed isoproteicdiets containing different amounts of non-structural carbohydrates (NSC, 36 vs 40 % DM). The lambs wereweighed both at birth (between February and March 2002) and at sale (about one month later) in order to estimate milkproduced by their mothers during the lactation period, by using a specific equation developed on Sarda ewes. Individualmilk yield and quality were then monitored monthly until the ewes dried off (July 2002). Our results showed that groupH-NSC (which received the diet with the highest NSC content) was associated to the highest milk yield (Pthe suckling period. In contrast, in the milking period group L-NSC showed significantly higher (P(996.2 vs 899.8 g/d) and fat and protein corrected milk yield (979.6 vs 877.7 g/d). Dietary treatments did not significantlyaffect milk composition.In conclusion, considering the milk production of each group as a function of the lactation month, group H-NSC had itsbest performance during the first two months of lactation, while group L-NSC was more productive during the remainingmonths. Also the percentage of protein, fat and casein of the milk during milking showed a similar trend. While the latterparameters were higher in the H-NSC group during the first two months of milking, from the 4th control onward higherlevels were observed in the L-NSC group. These results suggest, at least for Sarda primiparous ewes, the adoption ofdiets with different contents of non-structural carbohydrates according to the productive period (40 %, DM basis, in thefirst two months of lactation, 36 %, DM basis, in subsequent months).

In order to better define the effect of the sex on the metabolic profile of young ostriches (Struthio camelus domesticus), forty birds were divided into two groups by sex (20 males vs20 females). The animals were fed ad libitumnatural pasture and corn silage. The daily ration was completed by administering 1200 g/head of a commercial concentrate with the following chemical composition expressed as a percentage of dry mat- ter: crude protein 18.8, crude fibre 8.4, ether extract 3.6, ash 7.5. After about 12 h of fasting, in the mor- ning the blood was collected from the wing vein. The following biochemical parameters were determined: glu- cose, cholesterol, triglycerides, lactate (LAC), total protein (TP), uric acid, total bilirubin (Tbil), creatinine (CREA), calcium (Ca), magnesium (Mg), phosphorus (P), natrium (Na), potassium (K), chloride (Cl), iron (Fe), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AP), choline- sterase (ChE); α-amylase (Amyl), lipase (LIPA); γ-glutamyltransferase (GGT). Sex significantly affected only some haematic parameters: in the females total protein and calcium were higher than in the males (TP, 43.3 vs38.9 g/l, respectively for females and males, P< 0.05; Ca, 2.99 vs2.59 mmol/l, respectively for females and males, P< 0.01). The other haematic parameters did not show signifi- cant differences by sex, and the average values were: glucose (9.87 mmol/l), cholesterol (1.96 mmol/l), triglycerides (1.56 mmol/l), LAC (6.60 mmol/l), uric acid (361 mmol/l), CREA (31.95 µmol/l), Na (144.8 mmol/l), K (3.27 mmol/l), Cl (109.7 mmol/l), P (1.47 mmol/l), Mg (1.10 mmol/l), Fe (9.22 µmol/l), Tbil (9.28 µmol/l), AST (341.3 U/l), ALT (11.42 U/l), AP (75.8 U/l), GGT (10.07 U/l), Amyl (6.97 U/l), LIPA (241.2 U/l), ChE (385.1 U/l). The results of our study, in agreement with previous findings, contribute to enhance the knowledge on the metabolic profile of ostriches in function of the sex.

The aim of this study was to compare some somatic indexes, chemical-nutritive characteristics and the contents of some metals (Pb, Cu, Cr and Zn) in the whole body and fillet from caught and reared sharpsnout seabream (Diplodus puntazzo). The fish came from three different conditions: reared in marine cages (R), captured in a natural lagoon (L) and in the Mediterranean sea (S). Thirty fish per group, divided into three weight categories (100±15.3, 200±18.7 and 300±20.4g), were used for the trial. Reared sharpsnout seabream showed higher amounts of celomatic fat (3.41%, 2.43%, 0.21%, respectively for R, L and S) and total lipid (13.86%, 11.23% and 5.06% respectively for R, L and S), and lower moisture (64.14%, 65.54%, 71.53%) and protein (17.73, 19.03 and 19.17%) than those caught in the lagoon and sea. The whole body of reared fish contained lower amounts of lead (0.70, 0.75 and 0.97mg/kg, respectively for R, L and S), copper (0.15, 0.38, 0.25mg/kg) chrome (2.19, 3.52, 3.77mg/kg) and higher zinc contents (63.47, 53.42, 47.31mg/kg) than caught fish. Fatty acids from sharpsnout seabream fillets showed a high lipid quality as confirmed also by low values of Thrombogenic index (0.36, 0.30 and 0.22, respectively for L, S, R) and Atherogenic index (0.47, 0.42 and 0.33, respectively for L, S, R). Reared sharpsnout seabream showed lower saturated fatty acid values (26.44%, 32.21%, 34.85%, respectively for R, S, L) and higher oleic acid amount (21.61%, 19.15%, 11.99%, respectively for R, L and S). The subjects captured in the sea had a higher arachidonic acid content (5.44%, 1.76%, 0.59%, respectively for S, L, R). In the weight categories, the 100g subjects, showed higher incidence of viscera (VSI: 4.32%, 3.12% and 2.92%, respectively for 100, 200 and 300g) and liver (HIS: 2.20%, 1.97%, and 1.77%, respectively for 100, 200 and 300g), higher moisture (69.49%, 67.03%, 64.69%) and lower lipid rate (7.64%, 10.18%, 12.32%).

The aim of this trial was to evaluate the influence of two protein sources and the energy levels of the diet on growth performance and carcass quality of young Marchigiana bulls. Eighteen weaned young bulls (129 d of age) were equally divided into three groups. Until the slaughter weight (620 kg) was achieved two groups were fed diets with the same protein and energy concentrations, but differing in protein sources (faba bean-FB vs soya bean meal s.e.-SB), and the last group was fed high-energy diets (HE) with both protein sources in the concentrates. Body weight, daily weight gain and biological efficiency of growth at different ages and periods, and feed conversion indexes, were calculated from individual growth curves. Beginning at 180 d of age Group HE showed always significantly (P

The present study aimed to investigate the effect of storage duration of frozen inoculum on fermentation parametersobtained with the in vitro gas production technique. Two non-predigested diets differing in chemical composition andespecially crude fibre content (low fibre diet: 13.8%; high-fibre diet: 22.6%) were ground to pass a 1 mm screen andsubjected to fermentation with the same inoculum frozen for different periods: after 1 month (inoculum 1), after 2months (inoculum 2) and after 3 months (inoculum 3). The inoculum used was obtained from the caecal content of 75-day-old NZW rabbits. After defrosting, the caecal content was diluted with the medium 1:1 (V/V) and squeezed throughsix layers of gauze to obtain the inoculum. The substrate affected several fermentation parameters. In particular, thehigh-fibre diet had lower potential and cumulative gas production (A = ml/g 220 vs 256; P < 0.01 and OMCV ml/g 185vs 221; P < 0.01), lower organic matter degradability (OMl 67.2% vs 58.0%; P < 0.01) and production of volatile fattyacids (mmol/g 56.2 vs 49.8; P < 0.01), and took more time to obtain gas production equal to A/2 (B = h 11.8 vs 10.1;P < 0.01) compared with the low-fibre diet. However, the three inocula had very similar gas production kinetics, overlappingvalues of degraded organic matter (62.4%, 62.7% and 62.7% respectively for inocula 1, 2 and 3) and similarproduction of VFA (54.0, 52.2 and 52.8 mmol/g, respectively for inocula 1, 2 and 3). This research showed it is possibleto use frozen inoculum for at least 3 months and in this time interval obtain the same parameters of in vitro fermentation.

The aims of this study were to evaluate the correlation between in vitro crude protein digestibility coefficients of insect meals from Tenebrio molitor (TI) and Hermetia illucens (HI) and their chemical composition traits as well as to develop regression equations able to estimate the in vitro crude protein digestibility (CPd) from proximate analysis of insect meals. Twelve samples of insect meals (6 from TM larvae, TM 1-6 and 6 from HI larvae, HI 1-6) were obtained from different producers and analysed for chemical composition and in vitro crude protein digestibility by a two-step enzymatic method (digestion with pepsin and trypsin-enriched pancreatin). For both insect meal samples, CPd was negatively correlated to ADF and chitin contents, while just for HI there was a positive correlation (PTenebrio molitor samples and the 98.30% for Hermetia illucens. In the second step, the amount of protein linked to ADF was added in the model for T. molitor and CP for H. illucens samples. The coefficients chitin is the main constituent of insect body able to affect the crude protein digestibility of Tenebrio molitor and Hermetia illucens larvae meals estimated by an in vitro enzymatic method.

The aim of this study was to improve knowledge on the effect of group size on productive performance and egg quality of hens raised in furnished cages equally designed. A total of 520, 15-week-old Lohmann Brown laying hens were divided into 2 groups to have a similar initial body weight (average 1392±16.3 g). The cages of S25 group (240 L x 78 W x 50 H cm, 749 cm2/hen) hosted a total of 200 hens, while those of S40 group (462 L x 65 W x 50 H, 751 cm2/hen) included 320 birds. Experimental data were recorded after an adaptation period of 5 weeks (20 to 36 weeks of age). Hens were submitted to 15 h of light/d. The average temperature inside the building was 24.6±2.5°C over the entire experimental period with higher values at 24, 26, 28 and 30 weeks of age. The relative humidity recorded inside the building was 55% at week 20 and 60% all through the experimental period. Hens raised from S40 group had lower percentage of egg production (84.91 vs 88.90%, Pvs 2.25, Pvs 0.19%, P

In the present trial the fermentation characteristics of some grain legumes were studied using the in vitro gas production technique with a view to using them as an alternative protein source to soybean in animal feeding. Three cultivars of lupine, six cultivars of faba bean and seven cultivars of peas were incubated at 39°C with buffalo rumen fluid for 96h. OM degradability and fermentation kinetics were studied. Few differences in fermentation characteristics were observed among the cultivars for each legumes grains. “Scuro di Torre Lama” showed significantly (P

Four groups each consisting in 440, 60 days old rabbits, were fed, respectively, four experimental diets: (1) MOS_0.5 (Bio-Mos® at 0.5 g/kg); (2) MOS_1.0 (Bio-Mos® at 1.0 g/kg); (3) MOS_ 1.5 (Bio-Mos® at 1.5 g/kg) and (4) antibiotics (AGP, colistin sulphate 144 mg/kg; tylosin 100 mg/kg and oxytetracyclin 1000 mg/kg). Up to slaughter age (82 days of age) mortality rate was recorded daily. For each group, 64 rabbits were controlled weekly for live weight to calculate daily weight gain (DWG). Feed intake (and, by consequence feed conversion ratio) was measured, weekly, per group. At 82 days 16 rabbits per group were slaughtered and carcass traits were recorded. No differences were recorded among groups in live weight at different age and in daily weight gain but, in particular during the last week, AGP and MOS_0.5 groups showed higher feed intake and less favourable feed conversion ratio. MOS_1.0 group showed significantly higher incidence of empty gastro-intestinal tract but not differences were found for dressing out percentage. Perirenal fat showed a lower incidence in MOS than in AGP groups.

One hundred forty-four sharpsnout seabream of about 100 g initial body weight were randomly stocked in 12 experiment tanks (180 L). Testing conditions included 12 fish per tank, with triplicate tanks for treatment. The experimental period lasted 150 days. Average water temperature was 21.9±1.6°C, salinity was 30.0‰ and pH ranged from 7 to 8, throughout the experiment. A control diet (FM) was made from fish meal. One similar diet (SBM) was made with approximately 40% of the protein supplied by soybean meal. The remaining two diets (SBM-MOS and SBM-INU) were formulated adding 8 g of mannanoligosaccharide (MOS) and inulin (INU) per kg of the SBM diet, respectively. The results showed that mean final weight (average values 234.4 g), specific growth rate (average values 0.585), feed conversion rate (average values 2.05) and protein efficiency ratio (average values 1.01) were unaffected by MOS or INU supplementation to SBM diet. Body proximate composition was affected by MOS and INU supplementation. Fish fed SBM-MOS and SBM-INU diets showed the highest moisture level and the lowest lipid content. Also the total polyunsaturated fatty of the lipids was reduced by MOS and INU in comparison to SBM diet alone.

Shellfish aquaculture is a widespread activity in the Italian peninsula. However, only two bivalve species are mainly cultured along the coastline of that country: the Mediterranean mussel Mytilus galloprovincialis and the Manila clam Venerupis philippinarum (Ruditapes philippinarum). By contrast, just a few other mollusc species of commercial interest are scarcely reared at a small-scale level. After analysing the current status of Italian shellfish production, this paper reports and discusses the potential for culturing several different invertebrate species [i.e., the European flat oyster Ostrea edulis, the grooved carpet shell Venerupis decussata (Ruditapes decussatus), the razor clams Ensis minor and Solen marginatus, the cephalopod Octopus vulgaris, and the purple sea urchin Paracentrotus lividus] in this country. In addition, a detailed overview of the progress made in aquacultural techniques for these species in the Mediterranean basin is presented, highlighting the most relevant bottlenecks and the way forward to shift from the experimental to the aquaculture phase. Finally, an outlook of the main economic and environmental benefits arising from these shellfish culture practices is also given.

The present study was carried out in order to investigate the effects of the mannanoligosaccharide (MOS) and fructooligosaccharide (FOS) on sharpsnout seabream, Diplodus puntazzo in the context of partial fish meal substitution by soybean meal. One-hundred-forty-four sharpsnout seabream of about 100 g initial body weight were randomly divided in 12 experimental tanks (180 litre each). Testing conditions included 12 fish per tank, with triplicate tanks for treatment. The experimental period lasted 114 days. Average water temperature was 21.9±1.6°C, salinity was 30.0‰ and pH ranged from 7 to 8. Four isonitrogenous-isolipidic diets were tested: a control diet (FM) with fish meal as the sole protein source; a second diet (SBM) with approximately 40% of the protein supplied by soybean meal. The remaining two diets were formulated adding 8 g of MOS and FOS per kg of the SBM diet respectively. Average final weight, specific growth rate, feed conversion ratio and protein efficiency ratio remained unaffected by partial fish meal substitution and by MOS or FOS supplementation. Also apparent digestibility coefficients values for organic matter, protein, lipid and energy were not significantly affected by dietary treatment.

We assessed the percentage of endothelial progenitor cells (EPCs) in the peripheral blood of a patient with POEMS with elevated VEGF plasma levels. High VEGF plasma levels were associated with increased EPC concentration and treatment with an anti-VEGF antibody induced a consensual decrease of both parameters. In vitro cultures of the patient BM cells suggested that the stromal compartment could be responsible for VEGF overproduction.