11 results on '"Gillespie AV"'
Search Results
2. Measuring the impact of bovine digital dermatitis research on knowledge and practice of biosecurity during cattle foot-trimming.
- Author
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Gillespie AV, Carter SD, Blowey RW, Staton GJ, Walsh TR, and Evans NJ
- Subjects
- Animals, Cattle, Dairying methods, Disinfection, Farmers, Female, Health Knowledge, Attitudes, Practice, Surveys and Questionnaires, Veterinarians, Animal Husbandry instrumentation, Cattle Diseases prevention & control, Digital Dermatitis prevention & control, Hoof and Claw, Hygiene
- Abstract
This research communication reports the results from questionnaires used to identify the impact of recent research into the disinfection of cattle foot-trimming equipment to prevent bovine digital dermatitis (BDD) transmission on (a) biosecurity knowledge and (b) hygiene practice of foot health professionals. An initial questionnaire found that more than half of participating farmers, veterinary surgeons and commercial foot-trimmers were not considering hand or hoof-knife hygiene in their working practices. The following year, after the release of a foot-trimming hygiene protocol and a comprehensive knowledge exchange programme by the University of Liverpool, a second survey showed 35/80 (43.8%) farmers, veterinary surgeons and commercial foot-trimmers sampled considered they were now more aware of the risk of spreading BDD during foot- trimming. Furthermore, 36/80 (45.0%) had enhanced their hygiene practice in the last year, impacting an estimated 1383 farms and 5130 cows trimmed each week. Participants who reported having seen both the foot-trimming hygiene protocol we developed with AHDB Dairy and other articles about foot-trimming hygiene in the farming and veterinary press, were significantly more likely to have changed their working practices. Difficulties accessing water and cleaning facilities on farms were identified as the greatest barrier to improving biosecurity practices. Participants' preferred priority for future research was continued collection of evidence for the importance and efficacy of good foot-trimming hygiene practices.
- Published
- 2021
- Full Text
- View/download PDF
3. Removal of bovine digital dermatitis-associated treponemes from hoof knives after foot-trimming: a disinfection field study.
- Author
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Gillespie AV, Carter SD, Blowey RW, Staton GJ, and Evans NJ
- Subjects
- Animals, Cattle, Cattle Diseases prevention & control, DNA, Bacterial, Dairying instrumentation, Dairying methods, Digital Dermatitis transmission, Disinfectants, Female, Hoof and Claw, Iodophors chemistry, Peroxides chemistry, Sodium Hypochlorite chemistry, Sulfuric Acids chemistry, Treponema isolation & purification, Treponemal Infections prevention & control, Treponemal Infections veterinary, Digital Dermatitis prevention & control, Disinfection methods, Equipment Contamination prevention & control, Treponema drug effects
- Abstract
Background: Bovine digital dermatitis (BDD) is an infectious foot disease found commonly in dairy herds. Foot-trimming is an important husbandry procedure for reducing the ensuing lameness; however, epidemiological, and microbiological studies have identified this as a risk activity for transmitting BDD. Three disinfectants have previously been identified in laboratory work as effective for removing viable BDD-associated Treponema spp., from hoof knife blades. The present study enrolled 133 dairy cattle with BDD lesions, and swabbed hoof knife blades before and after foot-trimming, and after knife disinfection with one of three disinfectants (1:100 FAM30®, 2% Virkon® and 2% sodium hypochlorite) to assess their efficacy under field conditions., Results: Detection of BDD treponeme phylogroup DNA was undertaken by direct PCR of swabs, and viable treponemes were detected by PCR of swab cultures after 6 weeks' incubation. Where hoof knives did not contact the lesion, BDD-associated treponemes were detected after foot-trimming in 12/22 (54.5%) cases by direct PCR and 1/22 (4.5%) cases by PCR of cultured organisms. Where contact was made with the lesion, 111/111 (100%) samples taken after trimming were positive by direct PCR and 47/118 (39.8%) were positive by culture PCR. Viable organisms were identified in cultures from lesion stages M2, M3, M4 and M4.1. No viable organisms were detected after disinfection of hoof knives., Conclusions: Hoof knives post-trimming were frequently contaminated with BDD-associated treponeme DNA. Viable organisms were identified in cultures whether contact had been made between hoof knife and lesion or not, although contact clearly increased the frequency of detection of viable organisms. The three disinfectants tested were effective for removing viable organisms. The disinfection protocol used in this study should therefore be considered reliable for adoption as standard industry practice.
- Published
- 2020
- Full Text
- View/download PDF
4. Repeatability and Feasibility of Pressure Algometry for Quantifying Mechanical Nociceptive Threshold in the Thoracic Region of Calves.
- Author
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Williams HJ, Duncan JS, Grove-White DH, Mahen PJ, and Gillespie AV
- Abstract
Pressure algometry can be used to quantify mechanical nociceptive threshold (MNT) in humans and animals. If reliable this may be a useful tool to examine calves for increased mechanical sensitivity, which may be induced by disease or pain. This study measures the repeatability and feasibility of pressure algometry using a handheld digital pressure algometer (PRODPlus, Top Cat metrology) using three serial measurements applied to six sites on the thoraces of 35 healthy calves by two different operators. The range of MNTs recorded in healthy calves was 1.2-25 Newtons (median = 10.1 IQR = 7.1-14.0). A multivariable mixed effects model identified that the MNT's recorded were influenced by Operator, Site, and Calf. Intra and inter-operator reliability were measured by intra-class correlation coefficients (ICCs). Based on average ICCs, intra-operator reliability at two sites was good; one site overlying the ventral aspect of the 6th intercostal space [ICC = 0.79 95% CI (0.63-0.89)] and the other overlying the dorsal aspect of the 9th intercostal space [ICC = 0.75 95% CI (0.56-0.87)]. Average ICCs for three other measurement sites were moderate or poor, and one site proved unfeasible. For inter-operator agreement average ICCs showed that agreement was also good at the same 6 and 9th intercostal space, [ICCs = 0.77 95% CI (0.35-0.90) and 0.77 95% CI (0.54-0.88), respectively], agreement was moderate for the remainder of the sites. This study identifies two sites that are potentially useful for monitoring of thoracic sensitivity as an indicator of pain in calves by means of pressure algometry using the average of three measurements. It also identifies sources of variability to be considered when applying the tool for clinical or research purposes., (Copyright © 2020 Williams, Duncan, Grove-White, Mahen and Gillespie.)
- Published
- 2020
- Full Text
- View/download PDF
5. Effect of Calf Gender on Milk Yield and Fatty Acid Content in Holstein Dairy Cows.
- Author
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Gillespie AV, Ehrlich JL, and Grove-White DH
- Subjects
- Animals, Cattle, Datasets as Topic, Female, Male, Fatty Acids chemistry, Food Analysis, Milk chemistry
- Abstract
The scale of sexed semen use to avoid the birth of unwanted bull calves in the UK dairy industry depends on several economic factors. It has been suggested in other studies that calf gender may affect milk yield in Holsteins- something that would affect the economics of sexed semen use. The present study used a large milk recording data set to evaluate the effect of calf gender (both calf born and calf in utero) on both milk yield and saturated fat content. Linear regression was used to model data for first lactation and second lactation separately. Results showed that giving birth to a heifer calf conferred a 1% milk yield advantage in first lactation heifers, whilst giving birth to a bull calf conferred a 0.5% advantage in second lactation. Heifer calves were also associated with a 0.66kg reduction in saturated fatty acid content of milk in first lactation, but there was no significant difference between the genders in second lactation. No relationship was found between calf gender and milk mono- or polyunsaturated fatty acid content. The observed effects of calf gender on both yield and saturated fatty acid content was considered minor when compared to nutritional and genetic influences., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2017
- Full Text
- View/download PDF
6. Questionnaire regarding skin disorders in pet and smallholder pigs.
- Author
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Williams HJ, Grove-White DH, and Gillespie AV
- Subjects
- Animals, England epidemiology, Skin Diseases epidemiology, Skin Diseases therapy, Surveys and Questionnaires, Swine, Treatment Outcome, Pets, Skin Diseases veterinary, Swine Diseases epidemiology, Swine Diseases therapy
- Published
- 2016
- Full Text
- View/download PDF
7. Visual weight estimation and the risk of underdosing dairy cattle.
- Author
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van Dijk J, Eagle SJ, Gillespie AV, Smith RF, Holman AN, and Williams HJ
- Subjects
- Animals, Cattle, Female, Humans, Reproducibility of Results, Risk, Drug Dosage Calculations, Farmers psychology, Veterinarians psychology, Weight Perception
- Published
- 2015
- Full Text
- View/download PDF
8. Husbandry, health and biosecurity of the smallholder and pet pig population in England.
- Author
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Gillespie AV, Grove-White DH, and Williams HJ
- Subjects
- Animals, Communicable Diseases transmission, England, Feeding Methods veterinary, Government Agencies, Health Knowledge, Attitudes, Practice, Humans, Legislation, Veterinary, Registries statistics & numerical data, Risk Assessment, Surveys and Questionnaires, Travel legislation & jurisprudence, Vaccination veterinary, Animal Husbandry legislation & jurisprudence, Animal Welfare legislation & jurisprudence, Pets, Security Measures, Swine
- Abstract
Three hundred and thirteen pet and smallholder pig owners in England responded to an online questionnaire regarding husbandry and healthcare of their pigs. There was a lack of knowledge of the legislation regarding Department for Environment, Food and Rural Affairs (DEFRA) registration, animal movements and feeding of domestic food waste. Only 83.8 per cent of respondents had registered their pigs with DEFRA, while 17.7 per cent were not familiar with the movement regulations, and 23.9 per cent were feeding their pigs with household scraps. Contact with veterinary surgeons may be positively associated with DEFRA registration, legal feeding practices and knowledge of vaccination. Furthermore, the veterinary surgeon was considered to be the primary source of husbandry and healthcare knowledge. This paper identifies the pet and smallholder pig population as a potential risk for the incursion and spread of infectious disease, while highlighting the need for improved owner education., (British Veterinary Association.)
- Published
- 2015
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- View/download PDF
9. Outcome of surgical treatment for umbilical swellings in bovine youngstock.
- Author
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Williams HJ, Gillespie AV, Oultram JW, Cripps PJ, and Holman AN
- Subjects
- Animals, Cattle, Female, Hernia, Umbilical surgery, Male, Postoperative Complications veterinary, Prognosis, Recurrence, Surgical Mesh adverse effects, Surgical Mesh veterinary, Treatment Outcome, Umbilicus microbiology, Cattle Diseases surgery, Hernia, Umbilical veterinary, Umbilicus surgery
- Abstract
Umbilical swellings are commonly identified in calves and can be caused by hernia formation, infection of the remnants of umbilical vessels or a combination of both. Ninety-one cases with umbilical swellings were admitted to the Leahurst Farm Animal Practice (LFAP) between July 2004 and February 2012; 55 were simple hernias and 36 had associated infection. Eighty-seven cases underwent surgery of which 86 survived until discharge. Postoperative complications occurred in 65/86 animals (73 per cent). In 51 cases (81 per cent) this was classified as minor requiring no additional treatment. Placement of a prosthetic mesh was associated with a higher OR for developing severe postoperative complication when compared with those not receiving a mesh (OR=19.3; 95% CI 4.5 to 83.5). Long-term survival results were available for 49 animals, 22 of which were remaining in the herd with a median age of 1346 days (3.7 years). Of the 27 animals which had exited the herd, 16 were adult dairy cows, 7 were dairy heifers, 2 were beef animals and 2 exited at an unknown stage. Umbilical surgery in calves carries a good prognosis, although placement of a mesh increases the risk of complications occurring in the postoperative period.
- Published
- 2014
- Full Text
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10. The influence of sex upon the development of Friend virus leukaemia.
- Author
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Gillespie AV and Rowson KE
- Subjects
- Animals, Antibodies, Female, Leukemia, Experimental mortality, Male, Methods, Mice, Splenomegaly, Time Factors, Antibody Formation, Friend murine leukemia virus, Leukemia, Experimental etiology, Sex Factors
- Published
- 1968
- Full Text
- View/download PDF
11. Detection of a tumour-specific antigen (Gross) with the mixed antiglobulin reaction using erythrocytes from NZB-B1 mice.
- Author
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Gillespie AV
- Subjects
- Animals, Antigen-Antibody Reactions, Culture Techniques, Fluorescent Antibody Technique, Humans, Immune Sera, Methods, Mice, Antigens analysis, Coombs Test, Erythrocytes immunology, Neoplasms, Experimental immunology
- Published
- 1968
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