1. Selection of naturally occurring autoantibodies to interleukin-18 from phage display library
- Author
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Khrapov Ea, V. V. Morozova, Shveygert Mv, Gileva Ip, M. A. Vikhrova, Maksim L. Filipenko, and Nina V. Tikunova
- Subjects
Phage display ,Blotting, Western ,Molecular Sequence Data ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Biology ,Homology (biology) ,law.invention ,Peptide Library ,law ,Humans ,Immunology and Allergy ,Amino Acid Sequence ,Peptide library ,Gene ,Autoantibodies ,Interleukin-18 ,General Medicine ,Virology ,Molecular biology ,Recombinant Proteins ,Recombinant DNA ,biology.protein ,Immunoglobulin heavy chain ,Immunoglobulin Light Chains ,Antibody ,Immunoglobulin Heavy Chains ,Polymorphism, Restriction Fragment Length ,Single-Chain Antibodies - Abstract
Four unique phage single-chain variable fragments (scFvs) to recombinant human interleukin 18 (IL-18) has been selected from a naïve combinatorial library of human scFvs. Binding of unique phage antibodies with IL-18 was tested by ELISA and Western-blotting. No cross reactivity with tumor necrosis factor α, interferons α and γ was shown for the selected antibodies. The gene segments encoding V(D)J regions of selected antibodies exhibited a high degree of homology to germline genes, therefore we suggest that the selected scFv's belong to repertoire of naïve autoantibodies.
- Published
- 2010
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