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8. Abstract 1351: Lauryn Werner

Author

Werner, Lauryn Rose, primary, Gibson, Katelin A., additional, Goodman, Merit L., additional, Helm, Dominika E., additional, Walter, Katherine R., additional, Holloran, Sean M., additional, Trinca, Gloria M., additional, Hastings, Richard C., additional, Yang, Howard H., additional, Hu, Ying, additional, Wei, Junping, additional, Lei, Gangjun, additional, Yang, Xiao-Yi, additional, Madan, Rashna, additional, Molinolo, Alfredo A., additional, Markiewicz, Mary A., additional, Chalise, Prabhakar, additional, Axelrod, Margaret L., additional, Balko, Justin M., additional, Hunter, Kent W., additional, Hartman, Zachary C., additional, Lange, Carol A., additional, and Hagan, Christy R., additional

Published
2022
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9. Abstract P4-04-07: Progesterone promotes immunomodulation and tumor development in the murine mammary gland

Author

Werner, Lauryn R, primary, Gibson, Katelin A, additional, Goodman, Merit L, additional, Helm, Dominika E, additional, Walter, Katherine R, additional, Holloran, Sean M, additional, Trinca, Gloria M, additional, Hastings, Richard C, additional, Yang, Howard H, additional, Hu, Ying, additional, Wei, Junping, additional, Lei, Gangjun, additional, Yang, Xiao-Yi, additional, Madan, Rashna, additional, Molinolo, Alfred A, additional, Markiewicz, Mary A, additional, Chalise, Prabhakar, additional, Axelrod, Margaret L, additional, Balko, Justin M, additional, Hunter, Kent W, additional, Hartman, Zachary C, additional, Lange, Carol A, additional, and Hagan, Christy R, additional

Published
2022
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10. Progesterone promotes immunomodulation and tumor development in the murine mammary gland

Author

Werner, Lauryn R, primary, Gibson, Katelin A, additional, Goodman, Merit L, additional, Helm, Dominika E, additional, Walter, Katherine R, additional, Holloran, Sean M, additional, Trinca, Gloria M, additional, Hastings, Richard C, additional, Yang, Howard H, additional, Hu, Ying, additional, Wei, Junping, additional, Lei, Gangjun, additional, Yang, Xiao-Yi, additional, Madan, Rashna, additional, Molinolo, Alfredo A, additional, Markiewicz, Mary A, additional, Chalise, Prabhakar, additional, Axelrod, Margaret L, additional, Balko, Justin M, additional, Hunter, Kent W, additional, Hartman, Zachary C, additional, Lange, Carol A, additional, and Hagan, Christy R, additional

Published
2021
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15. In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos.

Author

Larson MA, Gibson KA, and Vivian JL

Subjects
Animals, Mice, Zygote growth & development, Blastocyst, CRISPR-Cas Systems genetics, Embryonic Development genetics, Gene Editing methods
Abstract

The CRISPR/Cas9 system has enjoyed enormous success and has now become the standard method of generating gene-modified mouse models. The tools for predicting the activity of CRISPR reagents in the mouse embryo are currently limited and not particularly accurate in predicting if a given reagent will be active. Given the time and cost of generating genetically modified mice, it is highly desirable to use CRISPR reagents that are known to be active in the mouse embryo. In this chapter, we provide a detailed procedure for empirically testing the activity of CRISPR reagents via electroporation into cultured preimplantation mouse embryos. This platform has proven to be rapid, efficient, and applicable to a variety of mouse strains, and can be used for assessing on- and off-target activity through a variety of molecular assays.

Published
2020
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