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Your search keyword '"Gibson, Angela L.F."' showing total 15 results
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15 results on '"Gibson, Angela L.F."'

3. What is Slough? A pilot study to define the proteomic and microbial composition of wound slough and its implications for wound healing

Author

Townsend, Elizabeth C., primary, Cheong, J. Z. Alex, additional, Radzietza, Michael, additional, Fritz, Blaine, additional, Malone, Matthew, additional, Bjarnsholt, Thomas, additional, Ousey, Karen, additional, Swanson, Terry, additional, Schultz, Gregory, additional, Gibson, Angela L.F., additional, and Kalan, Lindsay R., additional

Published
2023
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4. Pooled safety analysis of STRATA2011 and STRATA2016 clinical trials evaluating the use of StrataGraft® in patients with deep partial-thickness thermal burns

Author

Holmes IV, James H., primary, Cancio, Leopoldo C., additional, Carter, Jeffrey E., additional, Faucher, Lee D., additional, Foster, Kevin, additional, Hahn, Helen D., additional, King, Booker T., additional, Rutan, Randi, additional, Smiell, Janice M., additional, Wu, Richard, additional, and Gibson, Angela L.F., additional

Published
2022
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5. A phase 3, open-label, controlled, randomized, multicenter trial evaluating the efficacy and safety of StrataGraft® construct in patients with deep partial-thickness thermal burns

Author

Gibson, Angela L.F., primary, Holmes, James H., additional, Shupp, Jeffrey W., additional, Smith, David, additional, Joe, Victor, additional, Carson, Joshua, additional, Litt, Jeffrey, additional, Kahn, Steven, additional, Short, Tracee, additional, Cancio, Leopoldo, additional, Rizzo, Julie, additional, Carter, Jeffrey E., additional, Foster, Kevin, additional, Lokuta, Mary A., additional, Comer, Allen R., additional, Smiell, Janice M., additional, and Allen-Hoffmann, B. Lynn, additional

Published
2021
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8. Coming to Consensus : What Defines Deep Partial Thickness Burn Injuries in Porcine Models?

Author

Gibson, Angela L.F., Carney, Bonnie C., Cuttle, Leila, Andrews, Christine J., Kowalczewski, Christine J., Liu, Aiping, Powell, Heather M., Stone, Randolph, Supp, Dorothy M., Singer, Adam J., Shupp, Jeffrey W., Stalter, Lily, Moffatt, Lauren T., Gibson, Angela L.F., Carney, Bonnie C., Cuttle, Leila, Andrews, Christine J., Kowalczewski, Christine J., Liu, Aiping, Powell, Heather M., Stone, Randolph, Supp, Dorothy M., Singer, Adam J., Shupp, Jeffrey W., Stalter, Lily, and Moffatt, Lauren T.

Abstract

Deep partial thickness burns are clinically prevalent and difficult to diagnose. In order to develop methods to assess burn depth and therapies to treat deep partial thickness burns, reliable, accurate animal models are needed. The variety of animal models in the literature and the lack of precise details reported for the experimental procedures make comparison of research between investigators challenging and ultimately affect translation to patients. They sought to compare deep partial thickness porcine burn models from five well-established laboratories. In doing so, they uncovered a lack of consistency in approaches to the evaluation of burn injury depth that was present within and among various models. They then used an iterative process to develop a scoring rubric with an educational component to facilitate burn injury depth evaluation that improved reliability of the scoring. Using the developed rubric to re-score the five burn models, they found that all models created a deep partial thickness injury and that agreement about specific characteristics identified on histological staining was improved. Finally, they present consensus statements on the evaluation and interpretation of the microanatomy of deep partial thickness burns in pigs.

Published
2021

11. An open-label, prospective, randomized, controlled, multicenter, phase 1b study of StrataGraft skin tissue versus autografting in patients with deep partial-thickness thermal burns

Author

Holmes, James H., primary, Schurr, Michael J., additional, King, Booker T., additional, Foster, Kevin, additional, Faucher, Lee D., additional, Lokuta, Mary A., additional, Comer, Allen R., additional, Rooney, Peggy J., additional, Barbeau, Kelly F., additional, Mohoney, Stuart T., additional, Gibson, Angela L.F., additional, and Lynn Allen-Hoffmann, B., additional

Published
2019
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12. Discordance between histologic and visual assessment of tissue viability in excised burn wound tissue

Author

Karim, Aos S., Yan, Amy, Ocotl, Edgar, Bennett, Daniel D., Wang, Ziyue, Kendziorski, Christina, and Gibson, Angela L.F.

Subjects
Tissue Survival, Wound Healing, Trauma Severity Indices, Staining and Labeling, Sequence Analysis, RNA, Microcirculation, Humans, Regeneration, Burns, Coloring Agents, Article, Skin
Abstract

The regenerative capacity of burn wounds, and the need for surgical intervention, depends on wound depth. Clinical visual assessment is considered the gold standard for burn depth assessment but it remains a subjective and inaccurate method for tissue evaluation. The purpose of this study was to compare visual assessment with microscopic and molecular techniques for human burn depth determination, and illustrate differences in the evaluation of tissue for potential regenerative capacity. Using intraoperative visual assessment, patients were identified as having deep partial thickness or full thickness burn wounds. Tangential excisions of burn tissue were processed with hematoxylin and eosin to visualize tissue morphology, lactate dehydrogenase assay to ascertain cellular viability, and Keratin-15 and Ki67 to identify epidermal progenitor cells and proliferative capacity, respectively. RNA from deep partial and full thickness burn tissue as well as normal tissue controls were submitted for RNA sequencing. Lactate dehydrogenase, Keratin-15, and Ki67 were found throughout the excised burn wound tissue in both deep partial thickness burn tissues and in the second tangential excision of full thickness burn tissues. RNA sequencing demonstrated regenerative capacity in both deep partial and full thickness burn tissue, however a greater capacity for regeneration was present in deep partial thickness compared with full thickness burn tissues. In this study, we highlight the discordance that exists between the intraoperative clinical identification of burn injury depth, and microscopic and molecular determination of viability and regenerative capacity. Current methods utilizing visual assessment for depth of injury are imprecise, and can lead to removal of viable tissue. Additionally, hematoxylin and eosin microscopic analysis should not be used as the sole method in research or clinical determination of depth, as there are no differences in staining between viable and nonviable tissue.

Published
2018

15. Discordance between histologic and visual assessment of tissue viability in excised burn wound tissue.

Author

Karim, Aos S., Yan, Amy, Ocotl, Edgar, Bennett, Daniel D., Wang, Ziyue, Kendziorski, Christina, and Gibson, Angela L.F.

Subjects
TISSUE viability, BURNS & scalds, CELL proliferation, CYTOSKELETAL proteins, HISTOLOGICAL techniques, LACTATE dehydrogenase, MICROSCOPY, RNA, STAINS & staining (Microscopy), SURGICAL therapeutics, TRAUMATOLOGY diagnosis, CELL survival, SEQUENCE analysis, SURGERY
Abstract

The regenerative capacity of burn wounds, and the need for surgical intervention, depends on wound depth. Clinical visual assessment is considered the gold standard for burn depth assessment but it remains a subjective and inaccurate method for tissue evaluation. The purpose of this study was to compare visual assessment with microscopic and molecular techniques for human burn depth determination, and illustrate differences in the evaluation of tissue for potential regenerative capacity. Using intraoperative visual assessment, patients were identified as having deep partial thickness or full thickness burn wounds. Tangential excisions of burn tissue were processed with hematoxylin and eosin to visualize tissue morphology, lactate dehydrogenase assay to ascertain cellular viability, and Keratin‐15 and Ki67 to identify epidermal progenitor cells and proliferative capacity, respectively. RNA from deep partial and full thickness burn tissue as well as normal tissue controls were submitted for RNA sequencing. Lactate dehydrogenase, Keratin‐15, and Ki67 were found throughout the excised burn wound tissue in both deep partial thickness burn tissues and in the second tangential excision of full thickness burn tissues. RNA sequencing demonstrated regenerative capacity in both deep partial and full thickness burn tissue, however a greater capacity for regeneration was present in deep partial thickness compared with full thickness burn tissues. In this study, we highlight the discordance that exists between the intraoperative clinical identification of burn injury depth, and microscopic and molecular determination of viability and regenerative capacity. Current methods utilizing visual assessment for depth of injury are imprecise, and can lead to removal of viable tissue. Additionally, hematoxylin and eosin microscopic analysis should not be used as the sole method in research or clinical determination of depth, as there are no differences in staining between viable and nonviable tissue. [ABSTRACT FROM AUTHOR]

Published
2019
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