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219 results on '"Giantin M"'

16. Canine gastrointestinal stromal tumours treated with surgery and imatinib mesylate: three cases (2018‐2020).

Author

Treggiari, E., Giantin, M., Ferro, S., and Romanelli, G.

Subjects
GASTROINTESTINAL stromal tumors, TUMOR surgery, MAST cell tumors, IMATINIB, C-kit protein, THERAPEUTICS, SURGICAL excision
Abstract

Objectives: Gastrointestinal stromal tumours (GISTs) are described in dogs and are histologically diagnosed with the aid of immunohistochemistry to allow differentiation from leiomyomas/leiomyosarcomas. These tumours express c‐kit and in some cases could harbour mutations in KIT coding gene. Materials and Methods: Dogs with a diagnosis of GIST previously confirmed with histopathology and immunohistochemistry were considered for inclusion. Medical records were reviewed for clinical signs at presentation, results of diagnostic tests, tumour location and treatment. To be included, patients had to undergo staging procedures and treatment with imatinib alone or in combination with surgery. Immunohistochemistry and KIT mutational analysis were performed assessing all included cases. Results: Three cases were included. All cases underwent staging procedures and surgical excision. Tumours were located in the stomach (two cases) or caecum (one case). KIT mutational status was assessed and the presence of a 54‐base pair deletion in exon 11 was identified in one case. Following surgery, imatinib was used to treat recurrent, metastatic or residual disease and resulted in complete response and stable disease in the macroscopic setting and no evidence of recurrence in the microscopic setting. Follow‐up time was 890, 120 and 352 days, respectively. Clinical Significance: Surgical and medical treatment resulted in a positive outcome in these cases of canine GIST. Imatinib treatment was well tolerated and resulted in a measurable response and a low spectrum of toxicities. Further studies on the tolerability and efficacy of imatinib in solid tumours and GIST are warranted to define its effectiveness and safety. [ABSTRACT FROM AUTHOR]

Published
2023
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27. Transcriptome profiling of ruminants fed with a micronutrient-supplemented diet

Author

IANNACCONE M., IANNI A., ELGENDY R., PALAZZO F., GIANTIN M., GROTTA L., COSENZA G., DACASTO M., MARTINO G., Fulvia Bovera (Coordinator), Marzia Albenzio, Mariangela Caroprese, Rosaria Marino, Gianluca Neglia, Giovanni Piccolo, Angela Salzano, Iannaccone, M., Ianni, A., Elgendy, R., Palazzo, F., Giantin, M., Grotta, L., Cosenza, G., Dacasto, M., and Martino, G.

Subjects
ruminants fed, Transcriptome
Abstract

Iodine (I) is a micronutrient essential for the proper thyroid functionality in mammals. I requirement is dependent by the life stage and milk constitutes the main source of I intake for infant. For this reason, in dairy ruminants, the calibration of I is fundamental to keep the performance high. However, the effects of I supplementation on gene expression in dairy cow are not well documented. Thus, to improve knowledge in this field, we have assessed the effect of I supplementation dairy cattle using RNAsequencing. In detail, we have randomly divided 22 dairy cows into two groups: the first group (CTR, 11 animals) received a customised diet with physiological level of I2 (20 mg/day/animal of I2) while the second one (I, 11 animals) received the same diet with a I2 supplementation (20 + 65 mg/day/animal of I2) for 8 weeks (56 days). At the end of supplementation, the whole blood from both groups is been collected and the transcriptome analysis is been performed. Filtering our data using a FDR

Published
2019

28. Whole-transcriptome profiling of sheep fed with a high iodine-supplemented diet

Author

Iannaccone, M., Elgendy, Ramy, Ianni, A., Martino, C., Palazzo, F., Giantin, M., Grotta, L., Dacasto, M., Martino, G., Iannaccone, M., Elgendy, Ramy, Ianni, A., Martino, C., Palazzo, F., Giantin, M., Grotta, L., Dacasto, M., and Martino, G.

Abstract

Iodine (I) is a micronutrient that mammals need for proper functionality of thyroid gland since it is the main component of thyroid hormones. Besides studies that have investigated the role of I in livestock nutrition, it is also important to know the transcriptomics changes in small ruminants following I supplementation. Therefore, the aim of this study was to investigate the effects of I on the whole blood transcriptome in sheep. Fifteen lactating cross-bred ewes (3 to 4-year-old, 55 to 65 kg BW) at their late lactation period were enrolled in this study. At the beginning, all the animals had a 2-week acclimation period where they were fed with a basal diet which includes an adequate level of I (2 mg I/animal per day) in the form of calcium iodate (CaI2O6). Then, the ewes were randomly divided into two groups and fed in individual troughs: the control group (n = 5) was maintained on basal diet and the experimental group (I, n = 10) was fed for 40 days with a diet containing a high I supplementation (equivalent to 30 mg I/animal per day), in the form of potassium iodide. Whole blood and milk were collected individually at the beginning (T0) and after the 40 days of supplementation (T40). Iodine quantification was assessed in serum and milk sample. Microarray gene expression analysis was performed on whole blood and, filtering data using a fold change >2 with an adjusted P < 0.05, we identified 250 differentially expressed genes (DEGs) in the I group (T40 v. T0). Looking for biological processes associated with our DEGs, we found significant association with cell growth regulation. Thus, our study unveils the role of I supplementation on gene expression in sheep improving the knowledge about micronutrients in animal nutrition.

Published
2020
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