11 results on '"Gianfrani, Carmela"'
Search Results
2. Immunoregolatory pathways are active in the small intestinal mucosa of patients with potential celiac disease
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Borrelli M, Salvati MV, Zanzi D, Ferrara K, Santagata S, Ponticelli D, Mazzarella G, Lania G, MAGLIO, MARIANTONIA, AITORO, ROSITA, GIANFRANI, CARMELA, AURICCHIO, RENATA, TRONCONE, RICCARDO, Borrelli, M, Salvati, Mv, Maglio, Mariantonia, Zanzi, D, Ferrara, K, Santagata, S, Ponticelli, D, Aitoro, Rosita, Mazzarella, G, Lania, G, Gianfrani, Carmela, Auricchio, Renata, and Troncone, Riccardo
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- 2013
3. Probiotic bacteria enhance antigen sampling and processing by dendritic cells in pediatric IBD
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Strisciuglio, Caterina, primary, Miele, Erasmo, additional, Giugliano, Francesca Paola, additional, Vitale, Serena, additional, Andreozzi, Marialuisa, additional, Vitale, Alessandra, additional, Cenni, Sabrina, additional, Staiano, Annamaria, additional, Troncone, Riccardo, additional, and Gianfrani, Carmela, additional
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- 2014
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4. T300A Variant of Autophagy ATG16L1 Gene is Associated with Decreased Antigen Sampling and Processing by Dendritic Cells in Pediatric Crohnʼs Disease
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Strisciuglio, Caterina, primary, Miele, Erasmo, additional, Wildenberg, Manon E., additional, Giugliano, Francesca P., additional, Andreozzi, Marialuisa, additional, Vitale, Alessandra, additional, Capasso, Francesca, additional, Camarca, Alessandra, additional, Barone, Maria V., additional, Staiano, Annamaria, additional, Troncone, Riccardo, additional, and Gianfrani, Carmela, additional
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- 2013
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5. Intranasal administration of a recombinant α-gliadin down-regulates the immune response to wheat gliadin in DQ8 transgenic mice
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Senger, Stefania, Luongo, Diomira, Maurano, Francesco, Mazzeo, Maria F., Siciliano, Rosa A., Gianfrani, Carmela, David, Chella, Troncone, Riccardo, Auricchio, Salvatore, and Rossi, Mauro
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PRECANCEROUS conditions , *CELIAC disease treatment , *ANTIGENS - Abstract
The mucosal lesion in coeliac disease (CD) is an immune-mediated injury triggered by gliadin and associated with HLA-DQ2 and HLA-DQ8. In view of this, an approach that re-induces tolerance to this antigen should be considered as possible alternative to a strict gluten-free diet in treating CD. However, T-cell activation to multiple antigens, as a consequence of the chemical complexity shown by the antigen gliadin, could hamper the efforts to identify single component(s) useful for tolerance induction. To address this issue, a recombinant α-gliadin was tested in tolerance experiments in HLA-DQ8 transgenic mice. As tissue transglutaminase (tTG) treatment of gliadin, previously reported to enhance its stimulatory activity in CD, did not increase its immunogenicity when parenterally administered in these mice, untreated gliadin was used as immunogen. A decrease in systemic T cell responses to the recombinant α-gliadin was found after nasal administration of antigen, reflected by lymphocytes proliferation assay. Interestingly, while the immunisation protocol induced transcription of both Th1 (IFN-γ) and Th2 (IL-4 and IL-10) cytokines, the tolerisation protocol down-regulated significantly only the IFN-γ mRNA expression. More important, the recombinant α-gliadin induced a similar down-regulatory effect in mice immunised with a commercial preparation of wheat gliadin, that is a mixture of many different gliadin components. As the Th1 phenotype and the HLA-DQ8 molecule play a role in the pathogenesis of CD, our data underlined the potential usefulness of this recombinant protein for the immunomodulation of this disease. [Copyright &y& Elsevier]
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- 2003
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6. Gliadin-Specific T-Cells Mobilized in the Peripheral Blood of Coeliac Patients by Short Oral Gluten Challenge: Clinical Applications
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Roberta Mandile, Riccardo Troncone, Stefania Picascia, Carmen Gianfrani, Renata Auricchio, Picascia, Stefania, Mandile, R, Auricchio, Renata, Troncone, Riccardo, and Gianfrani, Carmela
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Glutens ,T-Lymphocytes ,Population ,lcsh:TX341-641 ,Review ,gluten challenge ,Gliadin ,Diet, Gluten-Free ,Interferon-gamma ,Intestinal mucosa ,interferon-γ ,Humans ,Medicine ,Genetic Predisposition to Disease ,Intestinal Mucosa ,education ,Triticum ,Randomized Controlled Trials as Topic ,chemistry.chemical_classification ,education.field_of_study ,Nutrition and Dietetics ,biology ,business.industry ,ELISPOT ,nutritional and metabolic diseases ,medicine.disease ,Gluten ,digestive system diseases ,celiac disease ,interferon-gamma ,Food intolerance ,Celiac Disease ,Lymphatic system ,chemistry ,Immunology ,biology.protein ,Gluten free ,business ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
Celiac disease (CD) is a common lifelong food intolerance triggered by dietary gluten affecting 1% of the general population. Gliadin-specific T-cell lines and T-cell clones obtained from intestinal biopsies have provided great support in the investigation of immuno-pathogenesis of CD. In the early 2000 a new in vivo, less invasive, approach was established aimed to evaluate the adaptive gliadin-specific T-cell response in peripheral blood of celiac patients on a gluten free diet. In fact, it has been demonstrated that three days of ingestion of wheat-containing food induces the mobilization of memory T lymphocytes reactive against gliadin from gut-associated lymphoid tissue into peripheral blood of CD patients. Such antigen-specific T-cells releasing interferon-γ can be transiently detected by using the enzyme-linked immunospot (ELISPOT) assays or by flow cytometry tetramer technology. This paper discusses the suitability of this in vivo tool to investigate the repertoire of gluten pathogenic peptides, to support CD diagnosis, and to assess the efficacy of novel therapeutic strategies. A systematic review of all potential applications of short oral gluten challenge is provided.
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- 2015
7. Lack of immunogenicity of hydrolysed wheat flour in patients with coeliac disease after a short-term oral challenge
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Renata Auricchio, R. Troncone, Alessandra Camarca, Stefania Picascia, Roberta Mandile, Luigi Greco, Carmen Gianfrani, C. Parrella, Marco Gobbetti, Mandile, R, Picascia, Stefania, Parrella, Claudia, Camarca, Alessandra, Gobbetti, M, Greco, Luigi, Troncone, Riccardo, Gianfrani, Carmela, and Auricchio, Renata
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Male ,0301 basic medicine ,Adolescent ,Glutens ,T-Lymphocytes ,Flour ,Wheat flour ,Peripheral blood mononuclear cell ,Gliadin ,Coeliac disease ,Diet, Gluten-Free ,Interferon-gamma ,03 medical and health sciences ,0302 clinical medicine ,Intestinal mucosa ,Humans ,Medicine ,Pharmacology (medical) ,Intestinal Mucosa ,Child ,Triticum ,chemistry.chemical_classification ,Hepatology ,biology ,business.industry ,Immunogenicity ,ELISPOT ,Fungi ,Gastroenterology ,nutritional and metabolic diseases ,food and beverages ,Bread ,medicine.disease ,Gluten ,digestive system diseases ,Celiac Disease ,Lactobacillus ,030104 developmental biology ,chemistry ,Fermentation ,Immunology ,Leukocytes, Mononuclear ,biology.protein ,Female ,030211 gastroenterology & hepatology ,business - Abstract
SummaryBackground A gluten-free diet is currently the only reliable therapeutic strategy that is approved for coeliac disease (CD). For many patients, however, compliance remains inadequate. Aim To investigate the immunogenicity of wheat flour that was pre-treated with selected lactobacilli and fungal proteases (hydrolysed wheat gluten) in coeliac patients. Methods The immunogenicity of hydrolysed wheat gluten was evaluated both in vitro in intestinal T cell lines (TCLs) and in vivo in treated CD patients after a short-term gluten challenge. Twenty treated CD patients were enrolled and equally randomised into two groups. The patients ate bread that was prepared with hydrolysed wheat flour or natural wheat flour (10 g of gluten/d for 3 days). The interferon (INF)-γ responses to natural gliadin and a 33-mer peptide were assessed by the enzyme-linked immunospot (ELISPOT) assay on peripheral blood mononuclear cells (PBMCs) both before and 6 days after the start of the challenge. Results Hydrolysed wheat was not able to activate the TCLs from the coeliac intestinal mucosa. Consistent with the in vitro results, no significant increase in INF-γ secretion was observed in patients who consumed hydrolysed wheat flour. Conversely, the consumption of natural wheat gluten mobilised INF-γ secreting cells in the blood (P
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- 2017
8. Immunogenic Peptides Can Be Detected in Whole Gluten by Transamidating Highly Susceptible Glutamine Residues: Implication in the Search for Gluten-free Cereals
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John Sidney, Alessandro Sette, Olga Fierro, Francesco Addeo, Giuseppe Mazzarella, Salvatore Auricchio, Riccardo Troncone, Gianfranco Mamone, Alessandra Camarca, Carmen Gianfrani, Mamone, G, Camarca, Alessandra, Fierro, O, Sidney, J, Mazzarella, G, Addeo, F, Auricchio, Salvatore, Troncone, Riccardo, Sette, A, and Gianfrani, Carmela
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Adult ,Adolescent ,Glutens ,Tissue transglutaminase ,Glutamine ,T-Lymphocytes ,Epitopes, T-Lymphocyte ,Gliadin ,Epitope ,Cell Line ,Young Adult ,Intestinal mucosa ,Antigen ,GTP-Binding Proteins ,Tandem Mass Spectrometry ,Humans ,Protein Glutamine gamma Glutamyltransferase 2 ,Intestinal Mucosa ,Triticum ,chemistry.chemical_classification ,Transglutaminases ,biology ,Immunogenicity ,food and beverages ,nutritional and metabolic diseases ,General Chemistry ,Middle Aged ,Gluten ,digestive system diseases ,Celiac Disease ,Biochemistry ,chemistry ,Antibody Formation ,biology.protein ,Gluten free ,Peptides ,General Agricultural and Biological Sciences - Abstract
Tissue transglutaminase (TG2) plays a central role in celiac disease (CD) pathogenesis by strongly enhancing the immunogenicity of gluten, the CD-triggering antigen. By deamidating specific glutamine (Q) residues, TG2 favors the binding of gluten peptides to DQ2/8 molecules and, subsequently, their recognition by cognate T cells. Six peptides were previously identified within wheat gliadin whole extracts by tagging the TG2-susceptible Q residues with monodansylcadaverine (MDC) and nanospray tandem mass spectrometry (nanoESI-MS/MS). The immunogenicity of these peptides was next tested in gliadin-specific T-cell lines established from CD intestinal mucosa. Four peptides, corresponding to known epitopes of ?- and ?-gliadins, induced cell proliferation and interferon (IFN)-? production. Interestingly, one of the two non-T-cell stimulatory peptides corresponded to the 31-49 ?-gliadin peptide implicated in the innate immune activation in CD mucosa. This study describes a strategy for identifying immunogenic gluten peptides potentially relevant for CD pathogenesis in protein extracts from wheat and other edible cereals.
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- 2013
9. In the Intestinal Mucosa of Children With Potential Celiac Disease IL-21 and IL-17A are Less Expressed than in the Active Disease
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Valentina Discepolo, D. Ponticelli, K. Ferrara, Merlin Nanayakkara, Mariantonia Maglio, Giuliana Lania, Carmen Gianfrani, Melissa Borrelli, Renata Auricchio, Delia Zanzi, Serena Vitale, Rosita Aitoro, Riccardo Troncone, Maria Vittoria Barone, Borrelli, Melissa, Gianfrani, Carmela, Lania, Giuliana, Aitoro, Rosita, Ferrara, K, Nanayakkara, Merlin, Ponticelli, D, Zanzi, Delia, Discepolo, Valentina, Vitale, Serena, Barone, MARIA VITTORIA, Troncone, Riccardo, Auricchio, Renata, and Maglio, Mariantonia
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Male ,Duodenum ,Disease ,03 medical and health sciences ,0302 clinical medicine ,Intestinal mucosa ,mucosal immunology ,Active disease ,IL-21 ,medicine ,Humans ,Intestinal Mucosa ,Child ,Cells, Cultured ,Hepatology ,business.industry ,Interleukins ,Interleukin-17 ,Gastroenterology ,nutritional and metabolic diseases ,Interleukin ,digestive system diseases ,Celiac Disease ,medicine.anatomical_structure ,Child, Preschool ,Immunology ,030211 gastroenterology & hepatology ,Female ,Interleukin 17 ,business ,030215 immunology - Abstract
OBJECTIVES: Potential celiac disease (CD) patients are at an increased risk to developing CD as indicated by positive CD-associated serology. We investigated in duodenal mucosa of such patients the presence of both IL-21 and IL-17A and the role of gliadin peptides and IL-15 in their expression. METHODS: Duodenal biopsies from 76 active CD, 90 potential CD, and 58 control patients were analyzed for IL-21 and/or IL-17A production by quantitative real-time PCR, immunohistochemistry, flow cytometry, and ELISA. The presence of IL-21 receptor was investigated by western blot. Potential CD duodenal fragments were cultured with gliadin peptides (PTG) and/or IL-15 and the expression/production of IL-21 and IL-17A assessed by quantitative real-time PCR and by immunohistochemistry. RESULTS: In potential CD, IL-21 was lower than in active CD, in terms of RNA expression (P
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- 2014
10. Short wheat challenge is a reproducible in-vivo assay to detect immune response to gluten
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Gaetano Terrone, G. Radano, Carmen Gianfrani, Francesco Maurano, Salvatore Auricchio, Riccardo Troncone, Alessandra Camarca, Luigi Greco, R. Di Mase, Camarca, Alessandra, Radano, G, DI MASE, Raffaella, Terrone, G, Maurano, F, Auricchio, Salvatore, Troncone, Riccardo, Greco, Luigi, and Gianfrani, Carmela
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Adult ,Male ,Time Factors ,Adolescent ,Glutens ,Immunology ,Human leukocyte antigen ,digestive system ,Coeliac disease ,Gliadin ,Epitopes ,Young Adult ,Immune system ,Antigen ,Interferon ,HLA-DQ Antigens ,Immunology and Allergy ,Medicine ,Humans ,Triticum ,chemistry.chemical_classification ,biology ,business.industry ,ELISPOT ,food and beverages ,nutritional and metabolic diseases ,Original Articles ,Antigens, Plant ,In-vivo challenge ,Interferon-? ,Peripheral blood ,Wheat gluten ,medicine.disease ,Gluten ,digestive system diseases ,Celiac Disease ,chemistry ,biology.protein ,Leukocytes, Mononuclear ,Female ,business ,Peptides ,medicine.drug - Abstract
Summary It has been reported that interferon (IFN)-γ-secreting T cells reactive to gluten can be detected in the peripheral blood of individuals with treated coeliac disease (CD) after a short consumption of wheat-containing food. By contrast, very little is known about the reproducibility of this in-vivo procedure in the same patient cohort which underwent two, or more, gluten consumptions. Fourteen coeliac patients in remission consumed wheat bread for 3 days; 13 underwent a second gluten challenge after a wash-out of 3–10 months on a strict gluten-free diet. Immune reactivity to gluten was analysed in peripheral blood by detecting IFN-γ before and 6 days after commencing a gluten diet. Gliadin-specific IFN-γ-secreting CD4+ T cells increased significantly on day 6 of the first challenge. These cells resulted as prevalently human leucocyte antigen (HLA)-DQ restricted and with a phenotype of gut homing, as suggested by the expression of β7-integrin. Similarly, reactiveness to gliadin was observed after the second wheat consumption, although with an individual variability of responses at each challenge. Our findings confirmed that the short wheat challenge is a non-invasive approach to investigate the gluten-related immune response in peripheral blood of subjects intolerant to gluten. Furthermore, we demonstrated that the in-vivo procedure can be reproduced in the same subject cohort after a gluten wash-out of at least 3 months. Our study has important implications for the application of this procedure to clinical practice.
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- 2012
11. Immunogenicity of Monococcum wheat in Celiac Patients
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Salvatore Auricchio, Nicola Giardullo, Riccardo Troncone, Norberto Pogna, Giuseppe Mazzarella, Gaetano Iaquinto, Mariatonia Maglio, Vera Rotondi Aufiero, Carmen Gianfrani, Alessandra Camarca, Immacolata Vocca, Gianfrani, Carmela, Maglio, Mariantonia, Rotondi Aufiero, V, Camarca, Alessandra, Vocca, I, Iaquinto, G, Giardullo, N, Pogna, N, Troncone, Riccardo, Auricchio, Salvatore, and Mazzarella, G.
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Triticum monococcum ,Nutrition and Dietetics ,biology ,celiac ,Cell growth ,T cell ,Medicine (miscellaneous) ,food and beverages ,Acquired immune system ,Immune system ,medicine.anatomical_structure ,Antigen ,Interleukin 15 ,Immunology ,biology.protein ,medicine ,Intraepithelial lymphocyte ,Gliadin ,innate immunity - Abstract
Background: Research is intense to find wheat of low or null toxicity for patients with celiac disease (CD). Among candidates, there are diploid wheat species. Objective: We compared the immunological properties of 2 lines of diploid monococcum wheat (Triticum monococcum ssp. monococcum), Monlis and ID331, with those of common wheat (Triticum aestivum). Design: Interferon-g production and the proliferation of intestinal gliadin-specific T cell lines and clones were measured as evidence of T cell activation by peptic and tryptic (PT) digests of gliadins from 2 monococcum lines. Furthermore, organ cultures of jejunal biopsies from 28 CD patients were set up to assess the effects of PT gliadin on innate and adaptive immune response by using immunohistochemistry. Results: Monlis and ID331 induced interferon-g production and proliferation in celiac mucosal T cells. In organ cultures, Monlis PT digest induced a significant increase of IL-15 epithelial expression and crypt enterocyte proliferation, whereas ID331 had no effect. Both monococcum lines caused intraepithelial T cell infiltration and lamina propria T cell activation. Conclusions: Our data show that the monococcum lines Monlis and ID331 activate the CD T cell response and suggest that these lines are toxic for celiac patients. However, ID331 is likely to be less effective in inducing CD because of its inability to activate the innate immune pathways. Am J Clin Nutr 2012;96:1339‐45.
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- 2012
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