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1. Real-world use of multigene signatures in early breast cancer: differences to clinical trials

Author

Licata, Luca, De Sanctis, Rita, Vingiani, Andrea, Cosentini, Deborah, Iorfida, Monica, Caremoli, Elena Rota, Sassi, Isabella, Fernandes, Bethania, Gianatti, Andrea, Guerini-Rocco, Elena, Zambelli, Claudia, Munzone, Elisabetta, Simoncini, Edda Lucia, Tondini, Carlo, Gentilini, Oreste Davide, Zambelli, Alberto, Pruneri, Giancarlo, and Bianchini, Giampaolo

Published
2024
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2. Non-Small Cell Lung Cancer Testing on Reference Specimens: An Italian Multicenter Experience

Author

Pepe, Francesco, Russo, Gianluca, Venuta, Alessandro, Scimone, Claudia, Nacchio, Mariantonia, Pisapia, Pasquale, Goteri, Gaia, Barbisan, Francesca, Chiappetta, Caterina, Pernazza, Angelina, Campagna, Domenico, Giordano, Marco, Perrone, Giuseppe, Sabarese, Giovanna, Altimari, Annalisa, de Biase, Dario, Tallini, Giovanni, Calistri, Daniele, Chiadini, Elisa, Capelli, Laura, Santinelli, Alfredo, Gulini, Anna Elisa, Pierpaoli, Elisa, Badiali, Manuela, Murru, Stefania, Murgia, Riccardo, Guerini Rocco, Elena, Venetis, Konstantinos, Fusco, Nicola, Morotti, Denise, Gianatti, Andrea, Furlan, Daniela, Rossi, Giulio, Melocchi, Laura, Russo, Maria, De Luca, Caterina, Palumbo, Lucia, Simonelli, Saverio, Maffè, Antonella, Francia di Celle, Paola, Venesio, Tiziana, Scatolini, Maria, Grosso, Enrico, Orecchia, Sara, Fassan, Matteo, Balistreri, Mariangela, Zulato, Elisabetta, Reghellin, Daniela, Lazzari, Elena, Santacatterina, Maria, Piredda, Maria Liliana, Riccardi, Manuela, Laurino, Licia, Roz, Elena, Longo, Domenico, Romeo, Daniela Petronilla, Fazzari, Carmine, Moreno-Manuel, Andrea, Puglia, Giuseppe Diego, Prjibelski, Andrey D., Shafranskaya, Daria, Righi, Luisella, Listì, Angela, Vitale, Domenico, Iaccarino, Antonino, Malapelle, Umberto, and Troncone, Giancarlo

Published
2024
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4. Procoagulant phenotype of virus-infected pericytes is associated with portal thrombosis and intrapulmonary vascular dilations in fatal COVID-19

Author

Cadamuro, Massimiliano, Lasagni, Alberto, Radu, Claudia Maria, Calistri, Arianna, Pilan, Matteo, Valle, Clarissa, Bonaffini, Pietro Andrea, Vitiello, Adriana, Toffanin, Serena, Venturin, Camilla, Friòn-Herrera, Yahima, Sironi, Sandro, Alessio, Maria Grazia, Previtali, Giulia, Seghezzi, Michela, Gianatti, Andrea, Strazzabosco, Mario, Strain, Alastair J., Campello, Elena, Spiezia, Luca, Palù, Giorgio, Frigo, Anna Chiara, Tosoni, Antonella, Nebuloni, Manuela, Parolin, Cristina, Sonzogni, Aurelio, Simioni, Paolo, and Fabris, Luca

Published
2024
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6. DeepMIF: Deep Learning Based Cell Profiling for Multispectral Immunofluorescence Images with Graphical User Interface

Author

Hagos, Yeman Brhane, Akarca, Ayse U, Ramsay, Alan, Rossi, Riccardo L, Pomplun, Sabine, Moioli, Alessia, Gianatti, Andrea, Mcnamara, Christopher, Rambaldi, Alessandro, Quezada, Sergio A., Linch, David, Gritti, Giuseppe, Marafioti, Teresa, Yuan, Yinyin, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Wang, Linwei, editor, Dou, Qi, editor, Fletcher, P. Thomas, editor, Speidel, Stefanie, editor, and Li, Shuo, editor

Published
2022
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8. In Situ Analyses of Placental Inflammatory Response to SARS-CoV-2 Infection in Cases of Mother–Fetus Vertical Transmission.

Author

Morotti, Denise, Tabano, Silvia, Gaudioso, Gabriella, Radaelli, Tatjana, Croci, Giorgio Alberto, Bianchi, Nicola, Ghirardi, Giulia, Gianatti, Andrea, Patanè, Luisa, Poletti de Chaurand, Valeria, Schwartz, David A., Hagazi, Mohamed A. A. A., and Grizzi, Fabio

Subjects
SECOND trimester of pregnancy, GENE expression, ASYMPTOMATIC patients, MULTIPLE pregnancy, TRANSCRIPTOMES, FETUS, PREMATURE rupture of fetal membranes
Abstract

It has been shown that vertical transmission of the SARS-CoV-2 strain is relatively rare, and there is still limited information on the specific impact of maternal SARS-CoV-2 infection on vertical transmission. The current study focuses on a transcriptomics analysis aimed at examining differences in gene expression between placentas from mother–newborn pairs affected by COVID-19 and those from unaffected controls. Additionally, it investigates the in situ expression of molecules involved in placental inflammation. The Papa Giovanni XXIII Hospital in Bergamo, Italy, has recorded three instances of intrauterine transmission of SARS-CoV-2. The first two cases occurred early in the pandemic and involved pregnant women in their third trimester who were diagnosed with SARS-CoV-2. The third case involved an asymptomatic woman in her second trimester with a twin pregnancy, who unfortunately delivered two stillborn fetuses due to the premature rupture of membranes. Transcriptomic analysis revealed significant differences in gene expression between the placentae of COVID-19-affected mother/newborn pairs and two matched controls. The infected and control placentae were matched for gestational age. According to the Benjamani–Hochberg method, 305 genes met the criterion of an adjusted p-value of less than 0.05, and 219 genes met the criterion of less than 0.01. Up-regulated genes involved in cell signaling (e.g., CCL20, C3, MARCO) and immune response (e.g., LILRA3, CXCL10, CD48, CD86, IL1RN, IL-18R1) suggest their potential role in the inflammatory response to SARS-CoV-2. RNAscope® technology, coupled with image analysis, was utilized to quantify the surface area covered by SARS-CoV-2, ACE2, IL-1β, IL-6, IL-8, IL-10, and TNF-α on both the maternal and fetal sides of the placenta. A non-statistically significant gradient for SARS-CoV-2 was observed, with a higher surface coverage on the fetal side (2.42 ± 3.71%) compared to the maternal side (0.74 ± 1.19%) of the placenta. Although not statistically significant, the surface area covered by ACE2 mRNA was higher on the maternal side (0.02 ± 0.04%) compared to the fetal side (0.01 ± 0.01%) of the placenta. IL-6 and IL-8 were more prevalent on the fetal side (0.03 ± 0.04% and 0.06 ± 0.08%, respectively) compared to the maternal side (0.02 ± 0.01% and 0.02 ± 0.02%, respectively). The mean surface areas of IL-1β and IL-10 were found to be equal on both the fetal (0.04 ± 0.04% and 0.01 ± 0.01%, respectively) and maternal sides of the placenta (0.04 ± 0.05% and 0.01 ± 0.01%, respectively). The mean surface area of TNF-α was found to be equal on both the fetal and maternal sides of the placenta (0.02 ± 0.02% and 0.02 ± 0.02%, respectively). On the maternal side, ACE-2 and all examined interleukins, but not TNF-α, exhibited an inverse mRNA amount compared to SARS-CoV-2. On the fetal side, ACE-2, IL-6 and IL-8 were inversely correlated with SARS-CoV-2 (r = −0.3, r = −0.1 and r = −0.4, respectively), while IL-1β and IL-10 showed positive correlations (r = 0.9, p = 0.005 and r = 0.5, respectively). TNF-α exhibited a positive correlation with SARS-CoV-2 on both maternal (r = 0.4) and fetal sides (r = 0.9) of the placenta. Further research is needed to evaluate the correlation between cell signaling and immune response genes in the placenta and the vertical transmission of SARS-CoV-2. Nonetheless, the current study extends our comprehension of the molecular and immunological factors involved in SARS-CoV-2 placental infection underlying maternal–fetal transmission. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Timing of sentinel node biopsy independently predicts disease-free and overall survival in clinical stage I-II melanoma patients: A multicentre study of the Italian Melanoma Intergroup (IMI)

Author

Mandalà, Mario, Galli, Francesca, Patuzzo, Roberto, Maurichi, Andrea, Mocellin, Simone, Rossi, Carlo R., Rulli, Eliana, Montesco, Maria, Quaglino, Pietro, Caliendo, Virginia, De Giorgi, Vincenzo, Merelli, Barbara, Caracò, Corrado, Piazzalunga, Dario, Labianca, Alice, Ribero, Simone, Senetta, Rebecca, Gianatti, Andrea, Valeri, Barbara, Massi, Daniela, Ascierto, Paolo A., and Santinami, Mario

Published
2020
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12. Macrophage expression and prognostic significance of the long pentraxin PTX3 in COVID-19

Author

Brunetta, Enrico, Folci, Marco, Bottazzi, Barbara, De Santis, Maria, Gritti, Giuseppe, Protti, Alessandro, Mapelli, Sarah N., Bonovas, Stefanos, Piovani, Daniele, Leone, Roberto, My, Ilaria, Zanon, Veronica, Spata, Gianmarco, Bacci, Monica, Supino, Domenico, Carnevale, Silvia, Sironi, Marina, Davoudian, Sadaf, Peano, Clelia, Landi, Francesco, Di Marco, Fabiano, Raimondi, Federico, Gianatti, Andrea, Angelini, Claudio, Rambaldi, Alessandro, Garlanda, Cecilia, Ciccarelli, Michele, Cecconi, Maurizio, and Mantovani, Alberto

Published
2021
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13. Machine learning streamlines the morphometric characterization and multi-class segmentation of nuclei in different follicular thyroid lesions: everything in a NUTSHELL

Author

L'Imperio, V, Coelho, V, Cazzaniga, G, Papetti, D, Del Carro, F, Capitoli, G, Marino, M, Ceku, J, Fusco, N, Ivanova, M, Gianatti, A, Nobile, M, Galimberti, S, Besozzi, D, Pagni, F, L'Imperio, Vincenzo, Coelho, Vasco, Cazzaniga, Giorgio, Papetti, Daniele M, Del Carro, Fabio, Capitoli, Giulia, Marino, Mario, Ceku, Joranda, Fusco, Nicola, Ivanova, Mariia, Gianatti, Andrea, Nobile, Marco S, Galimberti, Stefania, Besozzi, Daniela, Pagni, Fabio, L'Imperio, V, Coelho, V, Cazzaniga, G, Papetti, D, Del Carro, F, Capitoli, G, Marino, M, Ceku, J, Fusco, N, Ivanova, M, Gianatti, A, Nobile, M, Galimberti, S, Besozzi, D, Pagni, F, L'Imperio, Vincenzo, Coelho, Vasco, Cazzaniga, Giorgio, Papetti, Daniele M, Del Carro, Fabio, Capitoli, Giulia, Marino, Mario, Ceku, Joranda, Fusco, Nicola, Ivanova, Mariia, Gianatti, Andrea, Nobile, Marco S, Galimberti, Stefania, Besozzi, Daniela, and Pagni, Fabio

Abstract

The diagnostic assessment of thyroid nodules is hampered by the persistence of uncertainty in borderline cases, and further complicated by the inclusion of non-invasive follicular tumor with papillary-like nuclear features (NIFTP) as a less aggressive alternative to papillary thyroid carcinoma (PTC). In this setting, computational methods might facilitate the diagnostic process by unmasking key nuclear characteristics of NIFTPs. The main aims of this work were to (1) identify morphometric features of NIFTP and PTC that are interpretable for the human eye, and (2) develop a deep learning model for multi-class segmentation as a support tool to reduce diagnostic variability. Our findings confirmed that nuclei in NIFTP and PTC share multiple characteristics, setting them apart from hyperplastic nodules (HP). The morphometric analysis identified 15 features that can be translated into nuclear alterations readily understandable by pathologists, such as a remarkable inter-nuclear homogeneity for HP in contrast to a major complexity in the chromatin texture of NIFTP, and to the peculiar pattern of nuclear texture variability of PTC. A few NIFTP cases with available NGS data were also analyzed to initially explore the impact of RAS-related mutations on nuclear morphometry. Finally, a pixel-based deep learning model was trained and tested on whole slide images (WSIs) of NIFTP, PTC, and HP cases. The model, named NUTSHELL (NUclei from Thyroid tumors Segmentation to Highlight Encapsulated Low-malignant Lesions), successfully detected and classified the majority of nuclei in all WSIs' tiles, showing comparable results with already well-established pathology nuclear scores. NUTSHELL provides an immediate overview of NIFTP areas and can be used to detect microfoci of PTC within extensive glandular samples or identify lymph node metastases. NUTSHELL can be run inside WSInfer with an easy rendering in QuPath, thus facilitating the democratization of digital pathology.

Published
2024

17. Real-world use of multigene signatures in early breast cancer: differences to clinical trials

Author

Licata, Luca, primary, De Sanctis, Rita, additional, Vingiani, Andrea, additional, Cosentini, Deborah, additional, Iorfida, Monica, additional, Caremoli, Elena Rota, additional, Sassi, Isabella, additional, Fernandes, Bethania, additional, Gianatti, Andrea, additional, Guerini-Rocco, Elena, additional, Zambelli, Claudia, additional, Munzone, Elisabetta, additional, Simoncini, Edda Lucia, additional, Tondini, Carlo, additional, Gentilini, Oreste, additional, Zambelli, Alberto, additional, Pruneri, Giancarlo, additional, and Bianchini, Giampaolo, additional

Published
2023
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20. Effect of Radio-Chemotherapy on PD-L1 Immunohistochemical Expression in Head and Neck Squamous Cell Carcinoma

Author

Girolami, I, Marletta, S, Fiorentino, V, Battocchio, S, Cerbelli, B, Fiamengo, B, Gerosa, C, Gianatti, A, Morelli, L, Riva, G, Zagami, M, Fusco, N, Munari, E, L'Imperio, V, Pagni, F, Morbini, P, Martini, M, Eccher, A, Girolami, Ilaria, Marletta, Stefano, Fiorentino, Vincenzo, Battocchio, Simonetta, Cerbelli, Bruna, Fiamengo, Barbara, Gerosa, Clara, Gianatti, Andrea, Morelli, Luca, Riva, Giulio, Zagami, Maria Giovanna, Fusco, Nicola, Munari, Enrico, L'Imperio, Vincenzo, Pagni, Fabio, Morbini, Patrizia, Martini, Maurizio, Eccher, Albino, Girolami, I, Marletta, S, Fiorentino, V, Battocchio, S, Cerbelli, B, Fiamengo, B, Gerosa, C, Gianatti, A, Morelli, L, Riva, G, Zagami, M, Fusco, N, Munari, E, L'Imperio, V, Pagni, F, Morbini, P, Martini, M, Eccher, A, Girolami, Ilaria, Marletta, Stefano, Fiorentino, Vincenzo, Battocchio, Simonetta, Cerbelli, Bruna, Fiamengo, Barbara, Gerosa, Clara, Gianatti, Andrea, Morelli, Luca, Riva, Giulio, Zagami, Maria Giovanna, Fusco, Nicola, Munari, Enrico, L'Imperio, Vincenzo, Pagni, Fabio, Morbini, Patrizia, Martini, Maurizio, and Eccher, Albino

Abstract

Background: Programmed death-ligand 1 (PD-L1) checkpoint inhibitors represent a mainstay of therapy in head and neck squamous cell cancer (HNSCC). However, little is known about the influence of combined therapy on PD-L1 expression. The study aims to gather evidence on this topic. Methods: A systematic search was carried out in electronic databases Pubmed-MEDLINE and Embase to retrieve studies on the comparison of PD-L1 expression before and after conventional therapy. Data were extracted and a quantitative analysis with pooled odds ratios (ORs) was performed when applicable. Results: Of 5688 items, 15 were finally included. Only a minority of studies assessed PD-L1 with the recommended combined positive score (CPS). The results are highly heterogeneous, with some studies reporting an increase in PD-L1 expression and others reporting a decrease. Three studies allowed for quantitative analysis and showed a pooled OR of 0.49 (CI 0.27–0.90). Conclusions: From the present evidence, a clear conclusion towards an increase or decrease in PD-L1 expression after combined therapy cannot be drawn, but even with few studies available, a trend towards an increase in expression in tumor cells at a cutoff of 1% can be noted in patients undergoing platinum-based therapy. Future studies will provide more robust data on the effect of combined therapy on PD-L1 expression.

Published
2023

21. Effect of Radio-Chemotherapy on PD-L1 Immunohistochemical Expression in Head and Neck Squamous Cell Carcinoma

Author

Girolami, Ilaria, primary, Marletta, Stefano, additional, Fiorentino, Vincenzo, additional, Battocchio, Simonetta, additional, Cerbelli, Bruna, additional, Fiamengo, Barbara, additional, Gerosa, Clara, additional, Gianatti, Andrea, additional, Morelli, Luca, additional, Riva, Giulio, additional, Zagami, Maria Giovanna, additional, Fusco, Nicola, additional, Munari, Enrico, additional, L’Imperio, Vincenzo, additional, Pagni, Fabio, additional, Morbini, Patrizia, additional, Martini, Maurizio, additional, and Eccher, Albino, additional

Published
2023
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25. A prognostic model to predict survival in 867 World Health Organization–defined essential thrombocythemia at diagnosis: a study by the International Working Group on Myelofibrosis Research and Treatment

Author

Passamonti, Francesco, Thiele, Jürgen, Girodon, Francois, Rumi, Elisa, Carobbio, Alessandra, Gisslinger, Heinz, Kvasnicka, Hans Michael, Ruggeri, Marco, Randi, Maria Luigia, Gangat, Naseema, Vannucchi, Alessandro Maria, Gianatti, Andrea, Gisslinger, Bettina, Müllauer, Leonhard, Rodeghiero, Francesco, d'Amore, Emanuele S.G., Bertozzi, Irene, Hanson, Curtis A., Boveri, Emanuela, Marino, Filippo, Maffioli, Margherita, Caramazza, Domenica, Antonioli, Elisabetta, Carrai, Valentina, Buxhofer-Ausch, Veronika, Pascutto, Cristiana, Cazzola, Mario, Barbui, Tiziano, and Tefferi, Ayalew

Published
2012
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26. SARS-CoV-2 induces pericyte procoagulant response associated with portal vein microthrombosis and intrapulmonary vascular dilations in fatal COVID-19

Author

Lasagni, Alberto, primary, Cadamuro, Massimiliano, additional, Radu, Claudia Maria, additional, Calistri, Arianna, additional, Pilan, Matteo, additional, Valle, Clarissa, additional, Bonaffini, Pietro Angelo, additional, Vitiello, Adriana, additional, Sironi, Sandro, additional, Alessio, Maria Grazia, additional, Giulia, Previtali, additional, Seghezzi, Michela, additional, Gianatti, Andrea, additional, Strazzabosco, Mario, additional, Strain, Alastair J, additional, Campello, Elena, additional, Spiezia, Luca, additional, Palu, Giorgio, additional, Parolin, Cristina, additional, Sonzogni, Aurelio, additional, Simioni, Paolo, additional, and Fabris, Luca, additional

Published
2022
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31. High inter‐follicular spatial co‐localization of CD8+FOXP3+ with CD4+CD8+ cells predicts favorable outcome in follicular lymphoma

Author

Hagos, Yeman B., primary, Akarca, Ayse U., additional, Ramsay, Alan, additional, Rossi, Riccardo L., additional, Pomplun, Sabine, additional, Ngai, Victoria, additional, Moioli, Alessia, additional, Gianatti, Andrea, additional, Mcnamara, Christopher, additional, Rambaldi, Alessandro, additional, Quezada, Sergio A., additional, Linch, David, additional, Gritti, Giuseppe, additional, Yuan, Yinyin, additional, and Marafioti, Teresa, additional

Published
2022
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33. The Accuracy of Transurethral Bladder Resection in Detecting Bladder Cancer Histological Variants and Their Prognostic Value at Radical Cystectomy

Author

La Croce, Giovanni, primary, Naspro, Richard, additional, Finati, Marco, additional, Pellucchi, Federico, additional, Sodano, Mario, additional, Manica, Michele, additional, Catellani, Michele, additional, Gianatti, Andrea, additional, Roscigno, Marco, additional, and Da Pozzo, Luigi Filippo, additional

Published
2022
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34. RNAscope in situ hybridization and RT‐PCR for detection of SARS‐CoV‐2 in chilblain‐like lesions: A clinical, laboratory and histopathological study

Author

Robustelli Test, Elisa, primary, Sena, Paolo, additional, Locatelli, Andrea Gustavo, additional, Carugno, Andrea, additional, Mercurio, Marco, additional, Moggio, Erica, additional, Gambini, Daniele Mario, additional, Arosio, Marco Enrico Giovanni, additional, Callegaro, Annapaola, additional, Morotti, Denise, additional, Gianatti, Andrea, additional, and Vezzoli, Pamela, additional

Published
2022
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35. Pre-Transplant Alpha-Fetoprotein > 25.5 and Its Dynamic on Waitlist Are Predictors of HCC Recurrence after Liver Transplantation for Patients Meeting Milan Criteria

Author

Magro, Bianca, primary, Pinelli, Domenico, additional, De Giorgio, Massimo, additional, Lucà, Maria Grazia, additional, Ghirardi, Arianna, additional, Carrobio, Alessandra, additional, Baronio, Giuseppe, additional, Del Prete, Luca, additional, Nounamo, Franck, additional, Gianatti, Andrea, additional, Colledan, Michele, additional, and Fagiuoli, Stefano, additional

Published
2021
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36. NTRK Gene Fusion Detection in Atypical Spitz Tumors

Author

Cappellesso, Rocco, primary, Nozzoli, Filippo, additional, Zito Marino, Federica, additional, Simi, Sara, additional, Castiglione, Francesca, additional, De Giorgi, Vincenzo, additional, Cota, Carlo, additional, Senetta, Rebecca, additional, Scognamiglio, Giosuè, additional, Anniciello, Anna Maria, additional, Cesinaro, Anna Maria, additional, Mandalà, Mario, additional, Gianatti, Andrea, additional, Valente, Maria Gabriella, additional, Valeri, Barbara, additional, Sementa, Angela Rita, additional, Ricci, Costantino, additional, Corti, Barbara, additional, Roviello, Giandomenico, additional, Dei Tos, Angelo Paolo, additional, Franco, Renato, additional, and Massi, Daniela, additional

Published
2021
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37. Pathology of Immunotherapy-induced Responses in Cutaneous Melanoma: Current Evidences and Future Perspectives

Author

Indini, Alice, Lombardo, Maurizio, Sidoni, Angelo, Gianatti, Andrea, Mandalà, Mario, and Massi, Daniela

Abstract

Over the last years, immune checkpoint inhibitors (ICIs) have demonstrated remarkable anti-tumor activity and beneficial effects in patients with early and advanced melanoma. However, ICIs provide clinical benefit only in a minority of patients due to primary and/or acquired resistance mechanisms. Immunotherapy resistance is a complex phenomenon relying on genetic and epigenetic factors, which ultimately influence the interplay between cancer cells and the tumor microenvironment. Information is accumulating on the cellular and molecular mechanisms underlying the production of resistance and the resulting diminished therapeutic efficacy. In addition, current knowledge on predictors of response and toxicity to immunotherapy and on biomarkers that reliably identify resistant patients is in progress. In this review, we will focus on the tumor microenvironment changes induced by ICIs in melanoma, summarizing the available evidence of clinical trials in the neoadjuvant and metastatic setting. We will also overview the role of potential biomarkers in predicting disease response to ICIs, providing insight into current and future research in this field.

Published
2023
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38. A 57-year-old man with rapidly progressive pulmonary hypertension

Author

Raimondi, Federico, primary, Conti, Caterina, additional, Novelli, Luca, additional, Tarantini, Francesco, additional, Ciaravino, Giuseppe, additional, Scuri, Piermario, additional, Grosu, Aurelia, additional, Chinaglia, Daniela, additional, Grazioli, Lorenzo S.C., additional, Gianatti, Andrea, additional, Lorini, Ferdinando L., additional, Senni, Michele, additional, and Di Marco, Fabiano, additional

Published
2021
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39. Differential expression and regulation of MS4A family members in myeloid cells in physiological and pathological conditions

Author

Silva-Gomes, Rita, primary, Mapelli, Sarah N, additional, Boutet, Marie-Astrid, additional, Mattiola, Irene, additional, Sironi, Marina, additional, Grizzi, Fabio, additional, Colombo, Federico, additional, Supino, Domenico, additional, Carnevale, Silvia, additional, Pasqualini, Fabio, additional, Stravalaci, Matteo, additional, Porte, Rémi, additional, Gianatti, Andrea, additional, Pitzalis, Constantino, additional, Locati, Massimo, additional, Oliveira, Maria José, additional, Bottazzi, Barbara, additional, and Mantovani, Alberto, additional

Published
2021
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41. Additional file 1 of Covid-19 and gender: lower rate but same mortality of severe disease in women—an observational study

Author

Raimondi, Federico, Novelli, Luca, Ghirardi, Arianna, Russo, Filippo Maria, Pellegrini, Dario, Biza, Roberta, Trapasso, Roberta, Giuliani, Lisa, Anelli, Marisa, Amoroso, Mariangela, Allegri, Chiara, Imeri, Gianluca, Sanfilippo, Claudia, Comandini, Sofia, England Hila, Manesso, Leonardo, Gandini, Lucia, Mandelli, Pietro, Monti, Martina, Gori, Mauro, Senni, Michele, Lorini, Ferdinando Luca, Rizzi, Marco, Barbui, Tiziano, Paris, Laura, Rambaldi, Alessandro, Cosentini, Roberto, Guagliumi, Giulio, Cesa, Simonetta, Colledan, Michele, Sessa, Maria, Masciulli, Arianna, Gavazzi, Antonello, Buoro, Sabrina, Remuzzi, Giuseppe, Ruggenenti, Piero, Annapaola Callegaro, Gianatti, Andrea, Farina, Claudio, Bellasi, Antonio, Sironi, Sandro, Fagiuoli, Stefano, and Marco, Fabiano Di

Abstract

Additional file 1: Supplementary Definitions. Table S1. Intervals between symptoms and clinical relevant episodes in all patients and by gender. Table S2. 28-day outcomes according to gender and age.

Published
2021
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42. Additional file 2 of Covid-19 and gender: lower rate but same mortality of severe disease in women—an observational study

Author

Raimondi, Federico, Novelli, Luca, Ghirardi, Arianna, Russo, Filippo Maria, Pellegrini, Dario, Biza, Roberta, Trapasso, Roberta, Giuliani, Lisa, Anelli, Marisa, Amoroso, Mariangela, Allegri, Chiara, Imeri, Gianluca, Sanfilippo, Claudia, Comandini, Sofia, England Hila, Manesso, Leonardo, Gandini, Lucia, Mandelli, Pietro, Monti, Martina, Gori, Mauro, Senni, Michele, Lorini, Ferdinando Luca, Rizzi, Marco, Barbui, Tiziano, Paris, Laura, Rambaldi, Alessandro, Cosentini, Roberto, Guagliumi, Giulio, Cesa, Simonetta, Colledan, Michele, Sessa, Maria, Masciulli, Arianna, Gavazzi, Antonello, Buoro, Sabrina, Remuzzi, Giuseppe, Ruggenenti, Piero, Annapaola Callegaro, Gianatti, Andrea, Farina, Claudio, Bellasi, Antonio, Sironi, Sandro, Fagiuoli, Stefano, and Marco, Fabiano Di

Abstract

Additional file 2. Covid-19 Study Group.

Published
2021
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44. Successful Treatment of Suspected Donor-derived Human Herpesvirus-8 Infection in a Liver Transplant Patient With Coronavirus Disease-19

Author

Peri, Anna Maria, primary, Magro, Bianca, additional, van den Bogaart, Lorena, additional, dalla Pria, Alessia, additional, Giuffrida, Paolo, additional, Gianatti, Andrea, additional, Fabretti, Fabrizio, additional, Maria Barbui, Anna, additional, Tebaldi, Alessandra, additional, Rizzi, Marco, additional, and Fagiuoli, and Stefano, additional

Published
2021
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46. Cytopathology of Bronchoalveolar Lavages in COVID-19 Pneumonia: A Pilot Study

Author

Canini, V, Bono, F, Calzavacca, P, Capitoli, G, Foti, G, Fraggetta, F, Galimberti, S, Gianatti, A, Giani, M, Nasr, A, Paciocco, G, Pagni, F, Rona, R, L'Imperio, V, Canini, Valentina, Bono, Francesca, Calzavacca, Paolo, Capitoli, Giulia, Foti, Giuseppe, Fraggetta, Filippo, Galimberti, Stefania, Gianatti, Andrea, Giani, Marco, Nasr, Ahmed, Paciocco, Giuseppe, Pagni, Fabio, Rona, Roberto, L'Imperio, Vincenzo, Canini, V, Bono, F, Calzavacca, P, Capitoli, G, Foti, G, Fraggetta, F, Galimberti, S, Gianatti, A, Giani, M, Nasr, A, Paciocco, G, Pagni, F, Rona, R, L'Imperio, V, Canini, Valentina, Bono, Francesca, Calzavacca, Paolo, Capitoli, Giulia, Foti, Giuseppe, Fraggetta, Filippo, Galimberti, Stefania, Gianatti, Andrea, Giani, Marco, Nasr, Ahmed, Paciocco, Giuseppe, Pagni, Fabio, Rona, Roberto, and L'Imperio, Vincenzo

Abstract

BACKGROUND: Bronchoalveolar lavage (BAL) in patients with severe coronavirus disease 2019 (COVID-19) may provide additional and complementary findings for the management of these patients admitted to intensive care units (ICUs). This study addresses the cytological features of the infection and highlights the more influential inflammatory components. The correlation between pathological variables and clinical data is also analyzed. METHODS: The authors performed a retrospective analysis of the cytopathological features of BAL in 20 COVID-19 patients and 20 members of a matched cohort from a critical ICU who had acute respiratory distress syndrome caused by other pulmonary conditions. RESULTS: A comparison of the controls (n = 20) and the COVID-19 patients (n = 20) revealed that the latter had a higher neutrophil count (median, 63.8% of the cell count) with lower percentages of macrophages and lymphocytes. An increase in the expression of CD68-positive, monocytic multinucleated giant cells (MGCs) was reported; megakaryocytes were not detected on CD61 staining. Perls staining showed isolated elements. In situ RNA analysis demonstrated scattered chromogenic signals in type II pneumocytes. An ultrastructural analysis confirmed the presence of intracytoplasmic vacuoles containing rounded structures measuring 140 nm in diameter (putative viral particles). In COVID-19 patients, the clinicopathological correlation revealed a positive correlation between lactate dehydrogenase values and MGCs (r = 0.54). CONCLUSIONS: The analysis of BAL samples might be implemented as a routine practice for the evaluation of COVID-19 patients in ICUs in the appropriate clinical scenario. Additional studies using a larger sample size of patients who developed COVID-19 during the second wave of the epidemic in the autumn of 2020 are needed to further support our findings.

Published
2021

47. PD-L1 Testing and Squamous Cell Carcinoma of the Head and Neck: A Multicenter Study on the Diagnostic Reproducibility of Different Protocols

Author

Crosta, S, Boldorini, R, Bono, F, Brambilla, V, Dainese, E, Fusco, N, Gianatti, A, L'Imperio, V, Morbini, P, Pagni, F, Crosta, Simona, Boldorini, Renzo, Bono, Francesca, Brambilla, Virginia, Dainese, Emanuele, Fusco, Nicola, Gianatti, Andrea, L'Imperio, Vincenzo, Morbini, Patrizia, Pagni, Fabio, Crosta, S, Boldorini, R, Bono, F, Brambilla, V, Dainese, E, Fusco, N, Gianatti, A, L'Imperio, V, Morbini, P, Pagni, F, Crosta, Simona, Boldorini, Renzo, Bono, Francesca, Brambilla, Virginia, Dainese, Emanuele, Fusco, Nicola, Gianatti, Andrea, L'Imperio, Vincenzo, Morbini, Patrizia, and Pagni, Fabio

Abstract

Immune checkpoint inhibitors for blocking the programmed cell death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) axis are now available for squamous cell carcinoma of the head and neck (HNSCC) in relapsing and/or metastatic settings. In this work, we compared the resulting combined positive score (CPS) of PD-L1 using alternative methods adopted in routine clinical practice and determined the level of diagnostic agreement and inter-observer reliability in this setting. The study applied 5 different protocols on 40 tissue microarrays from HNSCC. The error rate of the individual protocols ranged from a minimum of 7% to a maximum of 21%, the sensitivity from 79% to 96%, and the specificity from 50% to 100%. In the intermediate group (1 ≤ CPS < 20), the majority of errors consisted of an underestimation of PD-L1 expression. In strong expressors, 5 out of 14 samples (36%) were correctly evaluated by all the protocols, but no protocol was able to correctly identify all the "strong expressors". The overall inter-observer agreement in PD-L1 CPS reached 87%. The inter-observer reliability was moderate, with an ICC of 0.774 (95% CI (0.651; 0.871)). In conclusion, our study showed moderate interobserver reliability among different protocols. In order to improve the performances, adequate specific training to evaluate PD-L1 by CPS in the HNSCC setting should be coordinated.

Published
2021

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