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Activated corrosion products generation in primary heat transfer systems of nuclear fusion facilities is a relevant radiological source term for occupation dose assessments. The formation of the Chalk River Undefined Deposit, already well known in nuclear fission power plants, represents a significant safety issue in fusion applications due to the intense high energy neutron fluences (about 14 MeV in Deuterium-Tritium operation). The activated corrosion products formation is a multi-physical problem. The combined synergy of activation, corrosion, dissolution, erosion, ejection, precipitation, and transport phenomena induces the contamination of coolant loop regions located outside the bio-shield, where scheduled worker operation might take place. The following manuscript shows how activated corrosion products are evaluated for the nuclear fusion power plant design under investigation by the Safety and Environment Work Package (WPSAE) of the Eurofusion Consortium (i.e., the European Demonstration power plant, EU-DEMO). The major issues in activated corrosion products estimations are here exposed and the main results for mass and activity inventories are briefly shown for some main Primary Heat Transfer Systems of EU-DEMO.

The aim of the present study is to model mixing and transport of radioactive effluents in the course of time between two water reservoirs. To test the model, a hypothetical case study is presented of liquid effluents potentially released during normal operation of a fusion neutron source devoted to radioisotopes production. A suitable example is an accelerator-driven intense D-T 14 MeV neutron source relying on T and D ion beams, with the potential to provide a neutron yield in the range $$ 5{-}7 \cdot 10^{13}$$ s-1. It is expected that during normal operation a number of radionuclides will be produced and managed. The present report discusses the mechanisms and parameters which affect and control the fate of radionuclides potentially released into two connected water reservoirs during normal operation of the plant. A mathematical mixing model is developed that describes groundwater flow and radioactive transport between the two basins. The aim of this study is to estimate the amount of radioactivity concentration in both water reservoirs at any time, an information that can be used for radiation protection purposes.

The decision to release a patient receiving medical treatments with unsealed radionuclides should take many factors into account, and is the balance among different requirements. The Italian legislative implementation of the new European Commission Basic Safety Standards (BSS) resulted in the recent Legislative Decree 101/2020, which sets specific dose constraints for the public and for carers and comforters. The aim of the present research is to assess acceptable release criteria (in terms of personal dose-equivalent rate at the moment of hospital discharge) to ensure compliance with the current regulatory framework in Italy. The MicroShield® code was used to derive a more realistic model for the emission of radioactivity by patients treated with radiopharmaceuticals. A geometric source-dependent correction factor, G (r ), is introduced in order to take into account the volumetric distribution of the radionuclide into the human body and the attenuation of radiation from body tissues. The proposed approach has the potential to be routinely applied by clinic decision-makers to assess release criteria in the nuclear medicine practice.

An experimental nuclear fusion device could be seen as a step toward the development of the future nuclear fusion power plant. If compared with other possible solutions to the energy problem, nuclear fusion has advantages that ensure sustainability and security. In particular, considering the radioactivity and the radioactive waste produced in a nuclear fusion plant, the component materials for the plant could be selected in order to limit the decay period, making recycling possible in a new reactor after about 100yr from the beginning of decommissioning. To achieve this and other pertinent goals, many experimental machines have been developed and operated worldwide in the last decades, underlining that radiation protection and worker exposure are critical aspects of these facilities due to the high-flux, high-energy neutrons produced in the fusion reactions. Direct radiation, material activation, tritium diffusion, and other related issues pose a real challenge to demonstrating that these devices are safer than nuclear fission facilities. In Italy, for the past 30yr, a limited number of fusion facilities have been constructed and operated, mainly at the ENEA Frascati Center, where a new one, the Italian Divertor Tokamak Test Facility (DTT), is now under development. The radiation protection approach, addressed by national licensing requirements, shows that respecting the constraints for worker exposure to ionizing radiation is not always straightforward. In the current analysis the main radiation protection issues encountered in the Italian fusion facilities are considered and discussed, and the technical and legal requirements are described. The licensing process for this kind of device is outlined and compared with that of other European countries. The following aspects are considered throughout the current study: description of the installation, plant, and systems; suitability of the area; buildings and structures; radioprotection structures and organization; exposure of personnel; accident analysis and relevant radiological consequences; and radioactive waste assessment and management. In conclusion, the analysis points out the need for special attention to the radiological exposure of workers in order to demonstrate at least the same level of safety as that reached at nuclear fission facilities.

The SORGENTINA-RF project aims at developing a 14 MeV fusion neutron source featuring an emission rate in the order of 5–7 × 1013 s−1. The plant relies on a metallic water-cooled rotating target and a deuterium (50%) and tritium (50%) ion beam. Beyond the main focus of medical radioisotope production, the source may represent a multi-purpose neutron facility by implementing a series of neutron-based techniques. Among the different engineering and technological issues to be addressed, the production of incondensable gases and corrosion product into the rotating target deserves a dedicated investigation. In this study, a preliminary analysis is carried out, considering the general layout of the target and the present choice of the target material.

In 2016 the Directive 2013/35/EU regarding the protection of health and safety of workers exposed to electromagnetic fields was transposed in Italy. Since then, the authors of this paper have been faced with several issues related to the implementation of the provisions of the Directive, which pose some interpretative and operative concerns. A primary critical feature of the Directive is that, in some circumstances, conditions of “overexposure”, i.e., of exceeding the exposure limits, are allowed. In the case of transient effects, the “flexibility” concerning the compliance with exposure limits is based on the approach introduced by ICNIRP in its guidelines on static magnetic fields and on time-varying electric and magnetic fields. On the contrary, the possibility of exceeding the exposure limits for health effects, formally recognized in the article of the Directive dealing with derogations, is not included in the ICNIRP guidelines. This paper analyzes the main concerns in interpreting and managing some provisions of the Directive with particular reference to the issue of how the employer can manage the situations of overexposure.

Portable neutron generators are one of the most widespread sources of fast neutrons for different applications, from activation analysis to quantitative estimation of fissile and fertile materials in radioactive waste packages. New techniques based on these devices have been developed for homeland security, e.g. neutron interrogations of packages suspected of containing substances such as explosives. When using portable generators determination of radiation protection quantities is affected by a series of boundary conditions that could differ from an experimental set-up to another, and Radiation Protection Experts cannot assess operator exposure without the aid of time-expensive Monte Carlo simulations. As it is impossible to foresee all kind of scenarios involving such applications, and time is often a critical variable, safety assessment requires faster, even if less accurate, tools for exposure evaluation. In this article experimental measurements of dose rates in a real scenario involving a D-T neutron generator are considered, to validate the Monte Carlo model developed. Consequently, it was possible to demonstrate that the calibration factor at 14 MeV for a neutron dosimeter provides conservative dose estimates and to propose a stand-alone, fast, and easy-to-use calculation tool which is usable on field by the Radiation Protection Expert without full Monte Carlo calculations.

ENEA is developing an accelerator-driven 14 MeV neutron source exploiting the deuterium–tritium fusion reaction to produce 99Mo medical radioisotope as an alternative production route not based on fission reactors. It is expected that, during normal operation, a number of radionuclides, generated by means of neutron irradiation on the raw material (natural Molybdenum), will be produced and managed. The present manuscript, as foreseen by national law, discusses a hypothetical scenario to test the environmental screening models, in turn evaluating the mechanisms and parameters which affect and control the path of liquid effluents potentially released during normal operation of the facility. The aim is to estimate the amount of radioactivity to be operated and the fraction potentially discharged in this hypothetical scenario, so as to ensure that the radioactive material can be managed without any risk for the population and the environment, according to national regulations and thoroughly fulfilling the international guidelines.

Abstarct The Divertor Tokamak Test facility (DTT) will operate with d -D plasmas producing pulses lasting about 60 s and producing a neutron yield up to 1.5 × 1017 n/s. Due to the high neutron yield, a significant activation of the structures and residual radioactivity after shut-down will be present. Consequently, DTT will need a robust radiation protection system to prevent undue worker and population exposure. The objectives of this system must be met during normal operation, maintenance, decommissioning and in emergency situations, thus several safety features must be routinely guarantee, among which the radiation monitoring system is probably the most crucial. This paper presents the general requirements of this monitoring system, giving some details about the preferred characteristics for the involved devices and monitors. The monitoring system shall be composed by portable instruments, passive ambient dosimeters and a limited number of fixed active measuring stations, suitable to detect and record the appropriate dosimetric quantities both for neutrons and photons. The active measuring stations and the passive dosimeters will constitute two monitoring networks designed in such a way to cover the area of interest to radiological survey around DTT.

The potential for a radiological or nuclear attack has been widely acknowledged in the last two decades. The use of a dirty bomb by terrorist organizations is considered to be a credible threat for which policymakers and relevant security agencies must prepare. Radioactive materials are stored in thousands of facilities around the world and may not be adequately protected against theft. This article analyzes a hypothetical dirty bomb attack in a large metropolitan area, evaluating the radiation dose to the involved population. The dispersion of radioactive materials is simulated using HOTSPOT code, considering a number of possible radionuclides (alpha, beta and gamma emitters) and scenarios. The findings of the present study corroborate and extend previous research demonstrating that it is unlikely that the atmospheric dispersion of radioactive material contained in a dirty bomb would produce deterministic effects in the exposed population. The radioactive material would be dispersed into the air resulting in relatively low doses. However, depending on the situation, the explosion of a dirty bomb is likely to contaminate properties (rendering them temporarily uninhabitable), thereby requiring potentially costly cleanup. Furthermore, due to the general fear of radiation, pervasive psychological effects are expected.

In order to properly respond to an emergency caused by an accident in a nuclear power plant with a spread of radionuclides in the atmosphere, we propose a field procedure to perform a large-scale individual thyroid monitoring of internal contamination due to inhalation of 131I, by means of non-spectrometric equipment, in particular dose rate meters. Specific attention is paid to the individual monitoring of children, because of the very high radiosensitivity of the child's thyroid to the carcinogenic effects of ionising radiation. The device performance was evaluated by measuring mock iodine sources provided in the Child and Adult Thyroid Monitoring After Reactor Accident (CAThyMARA) intercomparison and, just for a scintillator dose rate meter, by means of 60 s acquisitions of healthy volunteers' thyroids. All the devices showed a remarkable accuracy in quantification of equivalent 131I activity in the thyroids of persons of all ages. The selected scintillator dose rate meter showed detection limit values resulting in a maximum committed equivalent dose to thyroid HT, assuming an acute 131I inhalation occurred five days before the measurement, equal to 10 mSv (related to five-year-old children). Considering the level of HT values associated with the calculated detection limit activities, the proposed procedure has a significant sensitivity to be used for fast internally thyroid monitoring in nuclear or radiological emergencies, allowing daily monitoring a large amount of individuals.

Introduction Directive 2013/35/EU lays down minimum health and safety requirements regarding the exposure of workers to electromagnetic fields, including those present in Magnetic Resonance Imaging (MRI) facilities. For what concerns the Static Magnetic Field (SMF), the Directive follows a ‘flexible approach’ introduced by the 2009 ICNIRP guidelines, which is based on the distinction between sensory and health effects, thus allowing in some circumstances exposed workers to possibly experience unpleasant sensory effects. In addition, the Directive provides for the possibility of derogating from the health exposure limits, explicitly referring to the case of MRI. However, protection issues related to effects associated with movements of workers in the SMF are not completely addressed by EU limits. ICNIRP has published a guideline on this matter in 2014, but it has not yet been transposed into the Directive. Methods Measurements were performed of low frequency switched gradient magnetic fields and of the magnetic flux density experienced as variable by workers moving in the SMF of 1.5 T, 3 T and 7 T MRI scanners. In the latter case, three different metrics were applied to process the collected data and compare them with the EU Directive Exposure Limit Values (ELVs) for SMF, the ICNIRP basic restrictions (BRs) aimed at preventing vertigo effects and the ICNIRP reference levels (RLs) intended to prevent stimulation effects. Results Regarding movement in the SMF, reported values of the peak magnetic flux density, of its variation in 3 s and of the weighted-peak indices for stimulation effects show several cases of non-compliance with the corresponding ICNIRP BRs and RLs even when EU Directive ELVs are complied with. Results of exposure to low frequency magnetic fields are being processed. Acknowledgments part of the work presented is funded by INAIL, Italian Workers’ Compensation Authority.

Directive 2013/35/EU lays down minimum health and safety requirements regarding the exposure of workers to electromagnetic fields, including those present in Magnetic Resonance Imaging (MRI) facilities. For what concerns the Static Magnetic Field (SMF), the Directive follows a "flexible approach" introduced by the 2009 ICNIRP guidelines, which is based on the distinction between sensory and health effects, thus allowing in some circumstances exposed workers to possibly experience unpleasant sensory effects. In addition, the Directive provides for the possibility of derogating from the health exposure limits, explicitly referring to the case of MRI. However, protection issues related to effects associated with movements of workers in the SMF are not completely addressed by EU limits. ICNIRP has published a guideline on this matter in 2014, but it has not yet been transposed into the Directive. Measurements were performed of low frequency switched gradient magnetic fields and of the magnetic flux density experienced as variable by workers moving in the SMF of 1.5 T, 3 T and 7 T MRI scanners. In the latter case, three different metrics were applied to process the collected data and compare them with the EU Directive Exposure Limit Values (ELVs) for SMF, the ICNIRP basic restrictions (BRs) aimed at preventing vertigo effects and the ICNIRP reference levels (RLs) intended to prevent stimulation effects. Regarding movement in the SMF, reported values of the peak magnetic flux density, of its variation in 3 s and of the weighted-peak indices for stimulation effects show several cases of non-compliance with the corresponding ICNIRP BRs and RLs even when EU Directive ELVs are complied with. Results of exposure to low frequency magnetic fields are being processed.

The impact of climate change is more and more burdensome, not only because extreme weather events are increasing in frequency and intensity, but also because the severity of the impact depends on the vulnerability of the system. In particular, radiological hazards due to extreme weather events and climate-related disasters in hospitals and in many industries should be considered and possibly implemented into risk management policies. In the present study, the impact of climate change on human activities involving ionizing radiations in Italy is analyzed. Therefore, different scenarios related to extreme weather events are taken into account, in particular the case of flooding, an increasingly frequent event in Europe in recent years and now recognized as one of the most dangerous disasters. Management of these risks means focusing on preparing for and responding to emergencies. To this scope, the vulnerability of the considered radiological practices is assessed and appropriate actions to be undertaken are proposed and evaluated in order to secure the site or implement adequate safety measures. These adaptation strategies are needed to address the impact of climate change and should be included in the safety and emergency plans, according to the principle of optimization of radiation protection. © 2018, Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature.

The global climate is changing, as the earth becomes warmer, and the Mediterranean has been indicated among the regions most sensitive to climate change. The impact of climate change is more and more burdensome, not only because extreme weather events are increasing in frequency and intensity but also because the severity of the impact depends on the vulnerability of the system. Therefore, the consequences of events such as tornadoes and floods should be considered and managed in hospitals where radioactive substances are held and employed. In this article two cases of accident due to flooding or heavy rain in a nuclear medicine department are considered.

Background. The role of prolactin (PRL) on tissue injury and repair mechanisms in multiple sclerosis (MS) remains unclear. The aim of this work was to investigate the relationship between PRL plasma levels and brain damage as measured by magnetic resonance imaging (MRI).Methods. We employed a chemiluminescence immunoassay for measuring plasma levels of PRL. We used a 1.5 T scanner to acquire images and Jim 4.0 and SIENAX software to analyse them.Results. We included 106 women with relapsing remitting (RR) MS and stable disease in the last two months. There was no difference in PRL plasma levels between patients with and without gadolinium enhancement on MRI. PRL plasma levels correlated with white matter volume (WMV) (rho = 0.284,p=0.014) but not with grey matter volume (GMV). Moreover, PRL levels predicted changes in WMV (Beta: 984,p=0.034).Conclusions. Our data of a positive association between PRL serum levels and WMV support the role of PRL in promoting myelin repair as documented in animal models of demyelination. The lack of an increase of PRL in the presence of gadolinium enhancement, contrasts with the view considering this hormone as an immune-stimulating and detrimental factor in the inflammatory process associated with MS.

Purpose The exposure of operators moving in the static field of magnetic resonance (MR) facilities was assessed through measurements of the magnetic flux density, which is experienced as variable in time because of the movement. Collected data were processed to allow the comparison with most recent and authoritative safety standards. Methods Measurements of the experienced magnetic flux density B were performed using a probe worn by volunteers moving in MR environments. A total of 55 datasets were acquired nearby a 1.5T, 3T and 7T whole body scanners. Three different metrics were applied: the maximum intensity of B, to be compared with 2013/35/EU Directive exposure limit values for static fields; the maximum variation of the vector B on every 3s-interval, for comparison with the ICNIRP-2014 basic restriction aimed at preventing vertigo effects; two weighted peak indices (for “sensory” and “health” effects: SENS-WP, HLTH-WP), assessing compliance with ICNIRP-2014 and EU Directive recommendations intended to prevent stimulation effects. Results Peak values of |B| were greater than 2T in 9 of the 55 datasets. All the datasets at 1.5T and 3T were compliant with the limit for vertigo effects, whereas 6 datasets at 7T turned out to be non-compliant. At 7T, all 36 datasets were non-compliant for the SENS-WP index and 26 datasets even for the HLTH-WP one. Conclusions Results demonstrate that compliance with EU Directive limits for static fields does not guarantee compliance with ICNIRP-2014 reference levels and clearly show that movements in the static field could be the key component of the occupational exposure to EMF in MR facilities. This article is protected by copyright. All rights reserved.

The accident at Tokyo Electric Power Company's (TEPCO's) Fukushima Daiichi nuclear power plant (NPP) has been one of the dominant topic in nuclear safety and it has brought new attention on the matter of accidents in NPPs due to external events related to natural causes. Climate change has risen new risks and the growing probability of extreme external events has increased exposure and vulnerability of workers in the nuclear sector. However extreme natural events are a threat not only to NPPs but to all facilities dealing with radioactive material and in an emergency scenario they can affect the effectiveness and implementation of safety devices and procedures and also prevent communications, causing delays in the readiness of response. It is clear that adaptation strategies are necessary to cope with emerging changes in climate and a new nuclear safety culture is growing, that addresses accidents initiated not only by internal but also by external events.

Observed changes at the global scale. An increase of the annual mean global temperature and changes of other climate parameters have been observed in the last century. The global temperature and the atmospheric concentration of greenhouse gases are changing at a very fast pace compared to those found in palaeoclimate records. Changes in the Mediterranean. Variations of some climate change indicators can be much larger at the local than at the global scale, and the Mediterranean has been indicated among the regions most sensitive to climate change, also due to the increasing anthropogenic pressure. Model projections for the Mediterranean foresee further warming, droughts, and long-lasting modifications.Regional climate changes impact health and ecosystems, creating new risks, determined not only by weather events, but also by changing exposures and vulnerabilities. These issues, and in particular those regarding occupational safety, have not been sufficiently addressed to date.

The impacts of climate change (cc) are more and more burdensome, not only because extreme events are increasing in frequency and intensity, but also because the severity of the impact also depends on the vulnerability of the system. The accident at Fukushima Daiichi nuclear power plant (NPP) has brought new attention on the matter of adaptation of NPPs due to external events related to natural causes, but NPPs are not the only situations in which the use of ionizing radiation is affected by the impact of cc. In particular also at the hospitals the radiological risks due to cc extreme events and disasters have to be considered and managed.

A case study of exposure assessment of railway workers to static and extremely low frequency (ELF) magnetic fields is presented. A measurement campaign was conducted in aboard Italian main line trains. All measurements were performed on board during regular service (two engine drivers were simultaneously present), in all places potentially accessible to personnel, considering routes ranging from a few tens of kilometres to hundreds of kilometres. The measurement protocol was mostly based on broadband metres and personal metres were employed to assess individual exposure. Surveys on static and ELF magnetic fields were performed for seven different models of engine or electrified train. Traction motors were fed by alternating current (AC) current, except for two engines, where AC current fed only auxiliary services. The final result is that the average exposure to static magnetic field was a little higher than the background geomagnetic field; occasionally in few areas it could reach levels of the order of millitesla. The average exposure to ELF magnetic fields was in the order of 1-2 μT, with higher levels (few microtesla) only for one engine; occasionally in hot spots, close to wiring or specific equipment, the field values could reach several tens of microtesla. © The Author 2010. Published by Oxford University Press. All rights reserved.

BACKGROUND AND PURPOSE: Psychiatric symptoms occur in approximately 30% of patients with MS. Such symptoms include OCD, which may interfere heavily with the patient9s daily life. We hypothesized that the widespread involvement of both GM and WM, which characterizes MS, may be responsible for the occurrence of OCD when specific brain structures are affected. The aim of this study was to evaluate the relationship between GM and WM tissue damage and OCD in patients with MS. MATERIALS AND METHODS: We evaluated 16 patients with relapsing-remitting MS who had been diagnosed with OCD on the basis of the Diagnostic and Statistical Manual of Mental Disorders (4th edition) and 15 age- and sex-matched patients with relapsing-remitting MS with no psychiatric disorders as a CG. The MR study (1.5T) included 3D T1-weighted fast-field echo sequences, DTI (32 directions), and conventional MRI. Images were processed using SPM5, FSL, and Jim 5.0 software to evaluate VBM, TBSS, and global and regional LV, respectively. RESULTS: The VBM analysis revealed a set of clusters of reduced GM volume in the OCD group, compared with the CG, located in the right inferior and middle temporal gyri and in the inferior frontal gyrus. TBSS did not detect any differences in the FA values between the 2 groups; global and regional LV values also did not differ significantly between the 2 groups. CONCLUSIONS: Our study suggests that OCD in MS may be caused by damage in the right frontotemporal cortex.

The paper describes the cases of 3 female health operators with implanted copper IUDs, developing menometrorrhagia some months after an increase of the working time in a Magnetic Resonance Imaging (MRI) Unit (1.5 T), that progressively disappeared when the previous organization, involving discontinuous work shifts at MRI, was re-established. No known factors possibly related to menometrorrhagia were evidenced in the 3 operators, supporting the hypothesis of a role of the exposure to the electromagnetic fields (EMF) induced by the MRI system in symptoms induction. The possible mechanism remains unsettled, but menometrorrhagia might be triggered by a phlogistic stimulus caused by EMF, possibly the low-frequency currents induced in the wires of the IUD during the movements of the operator inside the static magnetic field generated by the MRI permanent magnet. Until now, the problem of possible interactions between copper IUDs and EMF induced by MRI has been considered in patients undergoing imaging, but the possible risk in MRI Units operators has been largely neglected. To our knowledge, the occurrence of menometrorrhagia is not routinely checked in health surveillance of MRI operators, so these symptoms can pass unnoticed, especially if they are transitory. Therefore, underreporting is rather possible. The cases described here support the need for further research on this topic, especially considering the progressive diffusion of more powerful MRI scanners (3 T and more), and of the interventional magnetic resonance imaging, both potentially involving higher EMF exposures, and a large number of MRI female operators, possibly using IUDs. The possibility that MRI operators with implanted metallic IUDs can be included in the group of “workers at particular risk” according to the EU Directive 2004/40/EC should be considered.

An inaccurate evaluation of exposure is considered a possible cause for the inadequate conclusiveness of epidemiological research on adverse effects of extremely low frequency-magnetic fields (ELF-MF). The objective of this study is to provide an evaluation of current ELF-MF exposure in workers, the specific contribution of occupational exposure to overall 24-h exposure, and the representativeness of a job exposure matrix (JEM). ELF-MF exposure was monitored in 543 workers for 2 days using personal meters. Time-weighted average (TWA) levels at work, at home and outside the home were calculated. A JEM based on the 1988 International Standard Classification of Occupations (ISCO 88) was created. Median exposure at work, at home and outside the home were 0.14, 0.03 and 0.05 μT, respectively. Occupational exposure accounted for about 60% of 24-h exposure. In the JEM, about 50% of the classified occupations included significantly different individual TWAs. Occupational exposure to ELF-MF appeared low. Median exposure levels at home and outside were 20-28% of the occupational level, giving a minor contribution to overall day-to-day exposure. The frequent occurrence of workers with different TWA included under the same job title highlights the risk of misclassification in epidemiological studies on ELF-MF effects based on JEM.

BACKGROUND AND PURPOSE: Findings of standard MR imaging examinations are usually normal in primary CD. These findings are now increasingly challenged by studies using advanced neuroimaging techniques detecting abnormalities in brain areas that may be functionally involved in the pathophysiology of CD. Our purpose was to evaluate GM volumes in patients with CD at baseline and 5 years later. MATERIALS AND METHODS: We enrolled 19 patients (F/M = 15:4, mean age = 53.2 + 11.2 years), 12 of whom were studied at baseline and again approximately 5 years later. Twenty-eight healthy volunteers acted as controls (F/M = 17:11, mean age = 47.5 + 15.6 years). The subjects were imaged with a 1.5T scanner by using a 3D T1-weighted sequence on 150 contiguous axial 1-mm-thick sections to apply VBM. RESULTS: At entry, VBM analysis disclosed significantly lower GM volumes in the left caudate head and putamen and in the premotor and primary sensorimotor cortices bilaterally in patients than in controls. No correlation was found between decreased GM volumes and patient age, severity of dystonia, or disease duration. At the 5-year follow-up, GM volumes in the left primary sensorimotor cortex in patients had decreased significantly from baseline. CONCLUSIONS: The findings obtained at entry and after a 5-year follow-up consistently showed decreased caudate, putamen, and sensorimotor cortex GM volumes in patients with CD, and they probably play a pathophysiologic role in CD.

Calmodulin, regulatory, and essential myosin light chain are evolutionary conserved proteins that, by binding to IQ motifs of target proteins, regulate essential intracellular processes among which are efficiency of secretory vesicles release at synapsis, intracellular signaling, and regulation of cell division. The yeast Saccharomyces cerevisiae calmodulin Cmd1 and the essential myosin light chain Mlc1p share the ability to interact with the class V myosin Myo2p and Myo4 and the class II myosin Myo1p. These myosins are required for vesicle, organelle, and mRNA transport, spindle orientation, and cytokinesis. We have used the budding yeast model system to study how calmodulin and essential myosin light chain selectively regulate class V myosin function. NMR structural analysis of uncomplexed Mlc1p and interaction studies with the first three IQ motifs of Myo2p show that the structural similarities between Mlc1p and the other members of the EF-hand superfamily of calmodulin-like proteins are mainly restricted to the C-lobe of these proteins. The N-lobe of Mlc1p presents a significantly compact and stable structure that is maintained both in the free and complexed states. The Mlc1p N-lobe interacts with the IQ motif in a manner that is regulated both by the IQ motifs sequence as well as by light chain structural features. These characteristic allows a distinctive interaction of Mlc1p with the first IQ motif of Myo2p when compared with calmodulin. This finding gives us a novel view of how calmodulin and essential light chain, through a differential binding to IQ1 of class V myosin motor, regulate this activity during vegetative growth and cytokinesis.

The revisited version of the HACACO experiment here presented, is more robust and straightforward to implement and continues to be, to a greater extent, a convenient tool for protein backbone resonance assignment. Additionally, it turns out to be a sensitive and accurate method to measure C(alpha)-H(alpha) residual dipolar couplings (RDCs). The performance of our new pulse scheme for measurement of RDCs was tested on two proteins with different secondary structures: one characterized by a high beta-sheet content, the second dominated by the presence of alpha-helices. In both examples the new method provided significantly more accurate data, compared to all previously published 3D techniques.

The NMR high-resolution structure of calmodulin complexed with a fragment of the olfactory cyclic-nucleotide gated channel is described. This structure shows features that are unique for this complex, including an active role of the linker connecting the N- and C-lobes of calmodulin upon binding of the peptide. Such linker is not only involved in the formation of an hydrophobic pocket to accommodate a bulky peptide residue, but it also provides a positively charged region complementary to a negative charge of the target. This complex of calmodulin with a target not belonging to the kinase family was used to test the residual dipolar coupling (RDC) approach for the determination of calmodulin binding modes to peptides. Although the complex here characterized belongs to the (1--14) family, high Q values were obtained with all the 1:1 complexes for which crystalline structures are available. Reduction of the RDC data set used for the correlation analysis to structured regions of the complex allowed a clear identification of the binding mode. Excluded regions comprise calcium binding loops and loops connecting the EF-hand motifs.

Purpose: A procedure for assessing occupational exposure due to magnetic resonance imaging (MRI) gradient magnetic fields and movement-induced effects in the static magnetic field is proposed and tested. Methods: The procedure was based on the application of theweighted-peak method in time domain. It was tested in two 1.5 T total-body and one 3 T head-only scanner MRI facilities in Rome (Italy). Exposure due to switched gradient fields was evaluated in locations inside the magnet room where operators usually stay during particular medical procedures (e.g., cardiac examinations of anesthetized patients); MRI sequences were selected to approach as far as possible a representative worst case exposure scenario. Movement-induced effects were evaluated considering the actual movements of volunteer operators during work activity, by measuring the perceived time-varying magnetic field by a head-worn probe. The analysis of results was based on ICNIRP 1998 and 2010 guidelines, following a weighted-peak approach and including an ad hoc extension to the latter ones, needed to verify compliance in the frequency range 0–1 Hz. Results: Exposures due to switched gradient fields in 1.5 T MRI scanners mostly resulted noncompliant with ICNIRP 1998 occupational reference levels, being, at the same time, always compliant with ICNIRP 2010 ones. Gradient field levels and ICNIRP indexes were significantly lower for the 3 T unit, due to its small dimensions, as that unit was a head-only scanner. Movement-induced effects resulted potentially noncompliant only in the case the operator moved the head inside the bore of a 1.5 T scanner. Conclusions: The procedure had proven to be a sound approach to exposure assessment in MRI. Its testing allowed to draw some general considerations about exposures to gradient magnetic fields and movement-induced effects.