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19 results on '"Ghosn, Bilal"'

4. Contributors

Author

Abramson, Sascha, primary, Ackermann, D. Michael, additional, Akins, Robert, additional, Anders, Richard, additional, Andersen, Phillip J., additional, Anderson, James M., additional, Ankrum, James A., additional, Anseth, Kristi S., additional, Antonucci, Joe, additional, Atzet, Sarah, additional, Badylak, Stephen F., additional, Baura, Gail D., additional, Bellamkonda, Ravi V., additional, Best, Serena M., additional, Bhumiratana, Sarindr, additional, Bianco, Richard W., additional, Bokros, Jack C., additional, Borovetz, Harvey S., additional, Boskey, Adele L., additional, Brown, Justin L., additional, Brown, Bryan N., additional, Brown, Stanley A., additional, Brunski, John B., additional, Cahn, Fred, additional, Ritchie, Alastair Campbell, additional, Caplan, Arnold I., additional, Carpenedo, Richard L., additional, Chilkoti, Ashutosh, additional, Chung, Sangwon, additional, Cimetta, Elisa, additional, Cleary, Gary, additional, Clements, Isaac P., additional, Colas, André, additional, Coleman, Kelly P., additional, Conway, Daniel E., additional, Cooper, Stuart L., additional, Costerton, Bill, additional, Coury, Arthur J., additional, Cunanan, Crystal, additional, Curtis, Jim, additional, D’Amore, Antonio, additional, DeMeo, Patrick, additional, Desai, Tejal A., additional, Dickens, Sabine, additional, Domingo, Gonzalo, additional, Duncan, Elaine, additional, Eskin, Suzanne G., additional, Feigal, David W., additional, Ferreira, Lino, additional, Fuller, Jason, additional, Gallegos, Robert P., additional, Gawalt, Ellen, additional, Ghosh, Kaustabh, additional, Ghosn, Bilal, additional, Gilbert, Thomas W., additional, Glaser, Drew Elizabeth, additional, Godier-Furnemont, Amandine, additional, Gombotz, Wayne R., additional, Grainger, David W., additional, Grunkemeier, Gary L., additional, Hacking, S. Adam, additional, Hallab, Nadim James, additional, Hall-Stoodley, Luanne, additional, Hanson, Stephen R., additional, Haubold, Axel D., additional, Hauch, Kip D., additional, Hawkins, Kenneth R., additional, Heath, Daniel E., additional, Helm, Douglas L., additional, Hench, Larry L., additional, Hensten, Arne, additional, Hill, Ryan T., additional, Hobson, Christopher, additional, Hoerstrup, Simon P., additional, Hoffman, Allan S., additional, Horbett, Thomas A., additional, Hubbell, Jeffrey A., additional, Humayun, Mark S., additional, Ideker, Ray, additional, Ingber, Donald E., additional, Jain, Rakhi, additional, Jacob, Jean, additional, Jacobs, Joshua James, additional, Jacobsen, Nils, additional, Jin, Ruyun, additional, Johnson, Richard J., additional, Karp, Jeffrey M., additional, Kasper, F. Kurtis, additional, Kathju, Sandeep, additional, Khademhosseini, Ali, additional, Kim, Sungwon, additional, King, Martin W., additional, Kleiner, Lothar W., additional, Kohn, Joachim, additional, Koschwanez, Heidi E., additional, Kumbar, Sangamesh G., additional, Kuo, Catherine K., additional, LaFleur, Lisa, additional, Lahti, Matthew T., additional, Lambert, Byron, additional, Langer, Robert, additional, Laurencin, Cato T., additional, Lee-Parritz, David, additional, Lemons, Jack E., additional, Levin, Mark, additional, Levy, Robert J., additional, Lewerenz, Gregory M., additional, Li, Wan-Ju, additional, Lin, Chien-Chi, additional, Liu, Fang, additional, Lowrie, William G., additional, Lu, Ying, additional, Lysaght, Michael J., additional, Maidhof, Robert, additional, Mansbridge, J.N., additional, Cristina, M., additional, Martins, L., additional, Martin, Jeffrey, additional, Mayesh, Jay P., additional, McDevitt, Todd C., additional, McIntire, Larry V., additional, Merrit, Katharine, additional, Migliaresi, Claudio, additional, Mikos, Antonios G., additional, Misch, Carl E., additional, Mitchell, Richard N., additional, More, Robert B., additional, Moss, Christa W., additional, Munson, Jennifer M., additional, Navarro, Melba, additional, Nerem, Robert M., additional, Ogawa, Rei, additional, Orgill, Britlyn D., additional, Orgill, Dennis P., additional, Padera, Robert F., additional, Pandit, Abhay, additional, Park, Kinam, additional, Patel, Anil S., additional, Peck, Roger B., additional, Peckham, P. Hunter, additional, Peppas, Nicholas A., additional, Pereira, Maria Nunes, additional, Planell, Josep, additional, Popat, Ketul C., additional, Prestwich, Glenn D., additional, Pun, Suzie H., additional, Rabolt, John, additional, Rainbow, Roshni S., additional, Rajab, Taufiek, additional, Ratner, Buddy D., additional, Reichert, William M., additional, Rivard, Andrew L., additional, Rowley, Adrian P., additional, Ruan, Gang, additional, Sacks, Michael, additional, Sarkar, Debanjan, additional, Schaefer, Sebastian, additional, Schmidt, Christine E., additional, Schoen, Frederick J., additional, Schutte, Stacey C., additional, Sefton, Michael V., additional, Shalaby, Shalaby W., additional, Shirtliff, Mark, additional, Simon, Marc A., additional, Singh, Milind, additional, Slack, Steven M., additional, Spelman, Francis A., additional, Starr, Albert, additional, Stayton, Patrick S., additional, Steinert, Roger, additional, Stoodley, Paul, additional, Suri, Shalu, additional, Swi Chang, Thomas Ming, additional, Tandon, Nina, additional, Tanguay, Armand R., additional, Taylor, M. Scott, additional, Teo, Grace S.L., additional, Thodeti, Charles K., additional, Tolkoff, Joshua, additional, Treiser, Matthew, additional, Tuan, Rocky S., additional, Tucker, Erik I., additional, Venugopalan, Ramakrishna, additional, Vicari, Angela R., additional, Viney, Christopher, additional, Voight, Jessica M., additional, Vunjak-Novakovic, Gordana, additional, Wagner, William R., additional, Wang, Lian, additional, Wasiluk, Karen R., additional, Watts, David Christopher, additional, Weigl, Bernhard H., additional, Weiland, James D., additional, Whalen, John J., additional, Williams, David F., additional, Williams, Rachel L., additional, Wilson, John T., additional, Wilson, Clive G., additional, Winter, Jessica, additional, Wolf, Michael F., additional, Wright, Jeremy C., additional, Yager, Paul, additional, and Zhao, Weian, additional

Published
2013
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5. Targeting

Author

Stayton, Patrick S., primary, Ghosn, Bilal, additional, and Wilson, John T., additional

Published
2013
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7. Using Project-Based, Experiential, and Service Learning in a Freshman Writing Intensive Seminar for Building Design and Technical Writing Skills (Work in Progress).

Author

Ghosn, Bilal and Volz, Tracy

Subjects
*TECHNICAL writing, *FACILITATED learning, *LEARNING ability, *ENGINEERING education, *ENGINEERING students, *LEARNING, *TECHNOLOGY education
Abstract

Writing and design are two key skills that engineering students should develop to achieve success. Most commonly, students are exposed to training for these skills separately in the early years of their collegiate coursework followed by their combination in the latter years of study, particularly in upper-level design courses. In fall of 2014, we introduced "Design for Disabilities: Engineering Design Principles for Minimizing Patient Limitations," a new first-year writing intensive seminar (FWIS), in an attempt to expose students to engineering design principles and technical communication in the context of an experiential-learning project early in their college careers. By doing this, we hoped to provide a strong foundation for the development of core competencies in our engineering students. [ABSTRACT FROM AUTHOR]

Published
2015

18. Development and evaluation of an imidazole-modified chitosan for nucleic acid and contrast agent delivery

Author

Ghosn, Bilal

Subjects
Delivery systems, Contrast agent delivery, Plasmid DNA, siRNA, Chitosan, Chitosan-IAA, Imidazole modified chitosan, Nucleic acid, Gene silencing, Gene expression
Abstract

Over the past several decades, gene therapy technologies have been developed for a diverse number of applications ranging from DNA-based vaccines to gene silencing with RNAi. While all are powerful tools, a common limitation for these technologies is the need for effective and safe delivery to target sites within the body. Such delivery vectors are necessary for retention of bioactivity and stability, while also providing a method of cellular and tissue uptake and distribution, which may require endosomal escape. Although, viral and lipid-based technologies have shown promise as nucleic acid delivery vectors, both have inherent issues such as cytoxicity, oncogenicity, and immunogenicity. Thus, the development of polymer-based non-viral vectors has been an area of great focus over the past decade. While many polymeric vectors have been developed for plasmid DNA (pDNA) delivery, very few have shown effective delivery of short interfering RNA (siRNA), a powerful tool for gene silencing via the RNA interference mechanism. Furthermore, very few prospective delivery vectors have shown versatility for the administration of siRNA through multiple routes of administration. The overall goal of this research was to develop a biocompatible non-viral delivery system for the delivery of plasmid DNA, siRNA, and contrast agents through the modification of the natural biopolymer chitosan. We have synthesized an imidazole modified chitosan (chitosan-IAA) by conjugation of imidazole acetic acid to chitosan. Extensive evaluation and characterization of the modified polymer demonstrates enhanced solubility and buffering capacity within the physiological and endosomal pHs, thus providing enhanced endosomal escape by exploiting the "proton sponge" effect. We have demonstrated effective in vitro gene expression and gene silencing with chitosan-IAA mediated delivery of pDNA and siRNA, respectively. Furthermore, we have demonstrated in vivo gene silencing by delivery of siRNA through both intranasal and intravenous routes of delivery with chitosan-IAA/siRNA nanocomplexes. We have also demonstrated delivery of contrast agents up to 45 nm in size through mucosal tissue following treatment with chitosan and no contrast agent modification in both human and animal tissue. In conclusion, we have successfully developed a versatile and highly effective delivery vector for both nucleic acids and contrast agents.

Published
2009

19. Enhancing polysaccharide-mediated delivery of nucleic acids through functionalization with secondary and tertiary amines.

Author

Ghosn B, Kasturi SP, and Roy K

Subjects
Amines adverse effects, Cell Line, Cell Survival drug effects, Chitosan adverse effects, Humans, Molecular Structure, Amines chemistry, Chitosan chemistry, Gene Transfer Techniques, Nanoparticles chemistry, Nucleic Acids administration & dosage
Abstract

Chitosan is a polysaccharide that has generated significant interest as a non-viral gene delivery vehicle due to its cationic and biocompatible characteristics. However, transfection efficiency of chitosan is significantly lower compared to other cationic gene delivery agents, e.g. polyethyleneimine (PEI), dendrimers or cationic lipids. This is primarily attributed to its minimal solubility and low buffering capacity at physiological pH leading to poor endosomal escape of the gene carrier and inefficient cytoplasmic decoupling of the complexed nucleic acid. Here we have developed an imidazole acetic acid (IAA)-modified chitosan to introduce secondary and tertiary amines to the polymer in order to improve its endosomal buffering and solubility. The modified polymer was characterized by ninhydrin and (1)H NMR assays for degree of modification, while buffering and solubility were analyzed by acid titration. Nanocomplex formation, studied at various polymer-nucleic acid ratios, showed an increase in particle zeta potential for chitosan-IAA, as well as an increase in the effective diameter. Up to 100-fold increase in transfection efficiency of pDNA was seen for chitosan-IAA as compared to native chitosan, nearly matching that of PEI. In addition, transfection of siRNA by the modified polymers showed efficient gene knockdown equivalent to commercially available siPORT Amines. Collectively, these results demonstrate the potential of the imidazole-grafted chitosan as a biocompatible and effective delivery vehicle for both pDNA and siRNA.

Published
2008
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