1. Systemic and Local Cytokine Profile in Biliary Atresia
- Author
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Saito, T., Sakamoto, A., Hatano, M., Iwai, J., Higashimoto, Y., Yoshida, H., Riepenhoff-Talty, M., Gouvea, V., Evans, M.J., Tyler, K.L., Sokol, R.J., Oberhaus, S.M., Desmet, V.J., Suskind, D.L., Rosenthal, P., Heyman, M.B., Petersen, C., Davenport, M., Shivakumar, P., Campbell, K.M., Sabla, G.E., Mack, C.L., Tucker, R.M., Lu, B.R., Bezerra, J.A., Tiao, G., Ryckman, F.C., Li, J, Bessho, K., Shinkai, M., Shinkai, T., Puri, P., Stringer, M.D., Nobili, V., Marcellini, M., Giovannelli, L., Wu, J.F., Kao, P.C., Chen, H.L., Urushihara, N., Iwagaki, H., Yagi, T., Narayanaswamy, B., Gonde, C., Tredger, J.M., Hussain, M., Vergani, D., Sakaguchi, S., Yamaguchi, T., Nomura, T., Ono, M., Lan, R.Y., Cheng, C., Lian, Z.X., Sakaki, M., Hiroishi, K., Baba, T., Yang, Y, Liu, Y.J., Tang, S.T., Brindley, S.M., Lanham, A.M., Karrer, F.M., Fontenot, A.P., Uchida, K., Inoue, M., Otake, K., Vejchapipat, P., Poomsawat, S., Chongsrisawat, V., Honsawek, S., Poovorawan, Y., Dong, R., Dong, K., Wang, X., Chen, G., Shen, C., Zheng, S., Mourya, R., Mieli-Vergani, G., Minnick, K.E., Kreisberg, R., Dillon, P.W., Ghoneim, E.M., Sira, M.M., Elaziz, A.M. Abd, Khalil, F.O., Sultan, M.M., Mahmoud, A.B., Fenoglio, D., Bernuzzi, F., Battaglia, F., Beriou, G., Costantino, C.M., Ashley, C.W., Koenen, H.J.P.M., Smeets, R.L., Vink, P.M., Rijssen, E. van, and Boots, A.M.
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Regulatory T cell ,medicine.medical_treatment ,Real-Time Polymerase Chain Reaction ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Biliary atresia ,Biliary Atresia ,Internal medicine ,medicine ,Humans ,Th1-Th2 Balance ,medicine.diagnostic_test ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,FOXP3 ,Infant ,medicine.disease ,Flow Cytometry ,Reverse transcription polymerase chain reaction ,030104 developmental biology ,medicine.anatomical_structure ,Real-time polymerase chain reaction ,Cytokine ,Endocrinology ,Liver ,Case-Control Studies ,Pediatrics, Perinatology and Child Health ,Cytokines ,030211 gastroenterology & hepatology ,Surgery ,business ,Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5] ,Biomarkers - Abstract
Purpose Systemic and local immune environments in human biliary atresia (BA) were analyzed. Methods Plasma concentrations of 19 inflammatory components in 16 preoperative BA patients (median age, 51 days), 13 normal controls (NCs) (44 days), and 15 cholestatic controls (CC) (23 days) were measured using flow cytometry, and compared according to post-Kasai outcomes in BA patients. Hepatic mRNA levels of representative helper T (Th) cell cytokines and forkhead box protein 3 (FoxP3) quantified by real-time reverse transcription polymerase chain reaction were compared between BA and non-BA. Results No significant differences were observed between BA and control in serum Th1, Th2, or macrophage markers, while soluble cellular adhesion molecule (CAM) levels were significantly higher in BA (p Conclusions A skewed bias toward specific Th-oriented immunity was not demonstrated in either the systemic or local environment in the early stage of human BA, with patient prognoses not necessarily revealed by preoperative plasma inflammatory component levels. CAM and regulatory T cell roles and functions warrant further investigation.
- Published
- 2016