Search

Your search keyword '"Ghia M"' showing total 193 results
Start Over Author "Ghia M"
193 results on '"Ghia M"'

1. Individual heritable differences result in unique cell lymphocyte receptor repertoires of naïve and antigen-experienced cells

Author

Florian Rubelt, Christopher R. Bolen, Helen M. McGuire, Jason A. Vander Heiden, Daniel Gadala-Maria, Mikhail Levin, Ghia M. Euskirchen, Murad R. Mamedov, Gary E. Swan, Cornelia L. Dekker, Lindsay G. Cowell, Steven H. Kleinstein, and Mark M. Davis

Subjects
Science
Abstract

The diversity of antigen receptor specificities is largely generated by random recombination of its segments. Here the authors show, by genetic comparison of monozygotic twin lymphocyte subsets, that individual genetic and epigenetic biases also contribute to the shape of the B and T cell repertoires.

Published
2016
Full Text
View/download PDF

2. Diverse roles and interactions of the SWI/SNF chromatin remodeling complex revealed using global approaches.

Author

Ghia M Euskirchen, Raymond K Auerbach, Eugene Davidov, Tara A Gianoulis, Guoneng Zhong, Joel Rozowsky, Nitin Bhardwaj, Mark B Gerstein, and Michael Snyder

Subjects
Genetics, QH426-470
Abstract

A systems understanding of nuclear organization and events is critical for determining how cells divide, differentiate, and respond to stimuli and for identifying the causes of diseases. Chromatin remodeling complexes such as SWI/SNF have been implicated in a wide variety of cellular processes including gene expression, nuclear organization, centromere function, and chromosomal stability, and mutations in SWI/SNF components have been linked to several types of cancer. To better understand the biological processes in which chromatin remodeling proteins participate, we globally mapped binding regions for several components of the SWI/SNF complex throughout the human genome using ChIP-Seq. SWI/SNF components were found to lie near regulatory elements integral to transcription (e.g. 5' ends, RNA Polymerases II and III, and enhancers) as well as regions critical for chromosome organization (e.g. CTCF, lamins, and DNA replication origins). Interestingly we also find that certain configurations of SWI/SNF subunits are associated with transcripts that have higher levels of expression, whereas other configurations of SWI/SNF factors are associated with transcripts that have lower levels of expression. To further elucidate the association of SWI/SNF subunits with each other as well as with other nuclear proteins, we also analyzed SWI/SNF immunoprecipitated complexes by mass spectrometry. Individual SWI/SNF factors are associated with their own family members, as well as with cellular constituents such as nuclear matrix proteins, key transcription factors, and centromere components, implying a ubiquitous role in gene regulation and nuclear function. We find an overrepresentation of both SWI/SNF-associated regions and proteins in cell cycle and chromosome organization. Taken together the results from our ChIP and immunoprecipitation experiments suggest that SWI/SNF facilitates gene regulation and genome function more broadly and through a greater diversity of interactions than previously appreciated.

Published
2011
Full Text
View/download PDF

3. Sequencing Y Chromosomes Resolves Discrepancy in Time to Common Ancestor of Males Versus Females

Author

Poznik, G David, Henn, Brenna M, Yee, Muh-Ching, Sliwerska, Elzbieta, Euskirchen, Ghia M, Lin, Alice A, Snyder, Michael, Quintana-Murci, Lluis, Kidd, Jeffrey M, Underhill, Peter A, and Bustamante, Carlos D

Subjects
Biological Sciences, Anthropology, Genetics, Human Society, Human Genome, Black People, Chromosomes, Human, Y, Evolution, Molecular, Female, Genetic Variation, Genome, Mitochondrial, Haploidy, Humans, Male, Mutation, Phylogeny, Sequence Analysis, DNA, Time Factors, General Science & Technology
Abstract

The Y chromosome and the mitochondrial genome have been used to estimate when the common patrilineal and matrilineal ancestors of humans lived. We sequenced the genomes of 69 males from nine populations, including two in which we find basal branches of the Y-chromosome tree. We identify ancient phylogenetic structure within African haplogroups and resolve a long-standing ambiguity deep within the tree. Applying equivalent methodologies to the Y chromosome and the mitochondrial genome, we estimate the time to the most recent common ancestor (T(MRCA)) of the Y chromosome to be 120 to 156 thousand years and the mitochondrial genome T(MRCA) to be 99 to 148 thousand years. Our findings suggest that, contrary to previous claims, male lineages do not coalesce significantly more recently than female lineages.

Published
2013

6. Risk of hospitalization for heart failure in patients with type 2 diabetes newly treated with DPP-4 inhibitors or other oral glucose-lowering medications: a retrospective registry study on 127,555 patients from the Nationwide OsMed Health-DB Database

Author

Fadini, Gian Paolo, Avogaro, Angelo, Degli Esposti, Luca, Russo, Pierluigi, Saragoni, Stefania, Buda, Stefano, Rosano, Giuseppe, Pecorelli, Sergio, Pani, Luca, Martinetti, S., Mero, P., Raeli, L., Migliazza, S., Dellagiovanna, M., Cerra, C., Gambera, M., Piccinelli, R., Zambetti, M., Atzeni, F., Valsecchi, V., DeLuca, P., Scopinaro, E., Moltoni, D., Pini, E., Leoni, O., Oria, C., Papagni, M., Nosetti, G., Caldiroli, E., Moser, V., Roni, R., Polverino, A., Bovo, C., Mezzalira, L., Andretta, M., Trentin, L., Palcic, S., Pettinelli, A., Arbo, A., Bertola, A., Capparoni, G., Cattaruzzi, C., Marcuzzo, L., Rosa, F.V., Basso, B., Saglietto, M., Delucis, S., Prioli, M., Filippi, R., Coccini, A., Ghia, M., Sanfelici, F., Radici, S., Scanavacca, P., Campi, A., Bianchi, S., Verzola, A., Morini, M., Borsari, M., Danielli, A., Dal Maso, M., Marsiglia, B., Vujovic, B., Pisani, M., Bonini, P., Lena, F., Aletti, P., Marcobelli, A., Sagratella, S., Fratini, S., Bartolini, F., Riccioni, G., Meneghini, A., Di Turi, R., Fano, V., Blasi, A., Pagnozzi, E., Quintavalle, G., DʼAvenia, P., De Matthaeis, M.C., Ferrante, F., Crescenzi, S., Marziale, L., Venditti, P., Bianchi, C., Senesi, I., Baci, R., De Carlo, I., Lavalle, A., Trofa, G., Marcello, G., Pagliaro, C., Troncone, C., Farina, G., Tari, M.G., Motola, G., De Luca, F., Saltarelli, M.L., Granieri, C., Vulnera, M., Palumbo, L., La Viola, F., Florio, L., De Francesco, A.E., Costantino, D., De Francesco, A.E., Rapisarda, F., Lazzaro, P.L., Pastorello, M., Parlli, M., Visconti, M., Uomo, I., Sanna, P., and Lombardo, F.

Published
2015
Full Text
View/download PDF

22. Advanced Age and Medication Prescription: More Years, Less Medications? A Nationwide Report From the Italian Medicines Agency

Author

Martinetti, S., Mero, P., Raeli, L., Migliazza, S., Dellagiovanna, M., Cerra, C., Gambera, M., Piccinelli, R., Zambetti, M., Atzeni, F., Valsecchi, V., DeLuca, P., Scopinaro, E., Moltoni, D., Pini, E., Leoni, O., Oria, C., Papagni, M., Nosetti, G., Caldiroli, E., Moser, V., Roni, R., Polverino, A., Bovo, C., Mezzalira, L., Andretta, M., Trentin, L., Palcic, S., Pettinelli, A., Arbo, A., Bertola, A., Capparoni, G., Cattaruzzi, C., Marcuzzo, L., Rosa, F.V., Basso, B., Saglietto, M., Delucis, S., Prioli, M., Filippi, R., Coccini, A., Ghia, M., Sanfelici, F., Radici, S., Scanavacca, P., Campi, A., Bianchi, S., Verzola, A., Morini, M., Borsari, M., Danielli, A., Dal Maso, M., Marsiglia, B., Vujovic, B., Pisani, M., Bonini, P., Lena, F., Aletti, P., Marcobelli, A., Sagratella, S., Fratini, S., Bartolini, F., Riccioni, G., Meneghini, A., Di Turi, R., Fano, V., Blasi, A., Pagnozzi, E., Quintavalle, G., D'Avenia, P., De Matthaeis, M.C., Ferrante, F., Crescenzi, S., Marziale, L., Venditti, P., Bianchi, C., Senesi, I., Baci, R., De Carlo, I., Lavalle, A., Trofa, G., Marcello, G., Pagliaro, C., Troncone, C., Farina, G., Tari, M.G., Motola, G., De Luca, F., Saltarelli, M.L., Granieri, C., Vulnera, M., Palumbo, L., La Viola, F., Florio, L., De Francesco, A.E., Costantino, D., Rapisarda, F., Lazzaro, P.L., Pastorello, M., Parelli, M., Visconti, M., Uomo, I., Sanna, P., Lombardo, F., Onder, Graziano, Marengoni, Alessandra, Russo, Pierluigi, Degli Esposti, Luca, Fini, Massimo, Monaco, Alessandro, Bonassi, Stefano, Palmer, Katie, Marrocco, Walter, Pozzi, Giuseppe, Sangiorgi, Diego, Buda, Stefano, Marchionni, Niccolò, Mammarella, Federica, Bernabei, Roberto, Pani, Luca, and Pecorelli, Sergio

Published
2016
Full Text
View/download PDF

24. Present Tense

Author

Ghia, M and Castore, L

Subjects
fotografia, estetica, teoria dell'immagine
Published
2015

26. Risk of hospitalization for heart failure in patients with type 2 diabetes newly treated with DPP-4 inhibitors or other oral glucose-lowering medications: A retrospective registry study on 127,555 patients from the Nationwide OsMed Health-DB Database

Author

Fadini, G. P., Avogaro, A., Degli Esposti, L., Russo, P., Saragoni, S., Buda, S., Rosano, G., Pecorelli, S., Pani, L., Martinetti, S., Mero, P., Raeli, L., Migliazza, S., Dellagiovanna, M., Cerra, C., Gambera, M., Piccinelli, R., Zambetti, M., Atzeni, F., Valsecchi, V., Deluca, P., Scopinaro, E., Moltoni, D., Pini, E., Leoni, O., Oria, C., Papagni, M., Nosetti, G., Caldiroli, E., Moser, V., Roni, R., Polverino, A., Bovo, C., Mezzalira, L., Andretta, M., Trentin, L., Palcic, S., Pettinelli, A., Arbo, A., Bertola, A., Capparoni, G., Cattaruzzi, C., Marcuzzo, L., Rosa, F. V., Basso, B., Saglietto, M., Delucis, S., Prioli, M., Filippi, R., Coccini, A., Ghia, M., Sanfelici, F., Radici, S., Scanavacca, P., Campi, A., Bianchi, S., Verzola, A., Morini, M., Borsari, M., Danielli, A., Dal Maso, M., Marsiglia, B., Vujovic, B., Pisani, M., Bonini, P., Lena, F., Aletti, P., Marcobelli, A., Sagratella, S., Fratini, S., Bartolini, F., Riccioni, G., Meneghini, A., Di Turi, R., Fano, V., Blasi, A., Pagnozzi, E., Quintavalle, G., D'Avenia, P., De Matthaeis, M. C., Ferrante, F., Crescenzi, S., Marziale, L., Venditti, P., Bianchi, C., Senesi, I., Baci, R., De Carlo, I., Lavalle, A., Trofa, G., Marcello, G., Pagliaro, C., Troncone, C., Farina, G., Tari, M. G., Motola, G., De Luca, F., Saltarelli, M. L., Granieri, C., Vulnera, M., Palumbo, L., La Viola, F., Florio, L., De Francesco, A. E., Costantino, D., Rapisarda, F., Lazzaro, P. L., Pastorello, M., Parlli, M., Visconti, M., Uomo, I., Sanna, P., and Lombardo, F.

Subjects
Adult, Male, medicine.medical_specialty, Epidemiology, Population, Administration, Oral, Medications, Heart failure, Type 2 diabetes, Lower risk, Diabetes, Incretin, Cardiology and Cardiovascular Medicine, Internal medicine, Diabetes mellitus, medicine, Humans, Hypoglycemic Agents, education, Aged, Retrospective Studies, education.field_of_study, Analysis of Variance, Dipeptidyl-Peptidase IV Inhibitors, business.industry, Hazard ratio, Absolute risk reduction, Retrospective cohort study, Middle Aged, medicine.disease, Hospitalization, Endocrinology, Sulfonylurea Compounds, Diabetes Mellitus, Type 2, Italy, Propensity score matching, Female, business, Diabetic Angiopathies
Abstract

Aims Oral glucose-lowering medications are associated with excess risk of heart failure (HF). Given the absence of comparative data among drug classes, we performed a retrospective study in 32 Health Services of 16 Italian regions accounting for a population of 18 million individuals, to assess the association between HF risk and use of sulphonylureas, DPP-4i, and glitazones. Methods and results We extracted data on patients with type 2 diabetes who initiated treatment with DPP-4i, thiazolidinediones, or sulphonylureas alone or in combination with metformin during an accrual time of 2 years. The endpoint was hospitalization for HF (HHF) occurring after the first 6 months of therapy, and the observation was extended for up to 4 years. A total of 127 555 patients were included, of whom 14.3% were on DPP-4i, 72.5% on sulphonylurea, 13.2% on thiazolidinediones, with average 70.7% being on metformin as combination therapy. Patients in the three groups differed significantly for baseline characteristics: age, sex, Charlson index, concurrent medications, and previous cardiovascular events. During an average 2.6-year follow-up, after adjusting for measured confounders, use of DPP-4i was associated with a reduced risk of HHF compared with sulphonylureas [hazard ratio (HR) 0.78; 95% confidence interval (CI) 0.62–0.97; P = 0.026]. After propensity matching, the analysis was restricted to 39 465 patients, and the use of DPP-4i was still associated with a lower risk of HHF (HR 0.70; 95% CI 0.52–0.94; P = 0.018). Conclusion In a very large observational study, the use of DPP-4i was associated with a reduced risk of HHF when compared with sulphonylureas.

Published
2015

28. Mapping of transcription factor binding regions in mammalian cells by ChIP: Comparison of array- and sequencing-based technologies

Author

Euskirchen, Ghia M., Rozowsky, Joel S., Chia-Lin Wei, Wah Heng Lee, Zhengdong, D. Zhang, Hartman, Stephen, Emanuelsson, Olof, Stolc, Viktor, Weissman, Sherman, Gerstein, Mark B., Yijun Ruan, and Snyder, Michael

Subjects
Cell cycle -- Research, Chromosome mapping -- Research, DNA microarrays -- Analysis, Nucleotide sequencing -- Analysis, Health
Abstract

The transcription factor binding region mapping is done by using chromatin immunoprecipitation (Chip) technique in DNA microarray analysis and in DNA sequencing. Both the techniques were found to be complementary to each other in their detection capacities.

Published
2007

29. Mapping the chromosomal targets of STAT1 by sequence Tag analysis of genomic enrichment (STAGE)

Author

Bhinge, Akshay A., Kim, Jonghwan, Euskirchen, Ghia M., Snyder, Michael, and Iyer, Vishwanath R.

Subjects
Cell proliferation -- Research, DNA-ligand interactions -- Research, Human genome -- Research, Genetic transcription -- Analysis, Health
Abstract

The sequence tag analysis of genomic enrichment (STAGE) used to identify transcription factor binding sites in the human genome is found to be more efficient than other techniques. It is vital in the cellular response such as cell proliferation, apoptosis and immune response.

Published
2007

30. Advanced Age and Medication Prescription: More Years, Less Medications? A Nationwide Report From the Italian Medicines Agency

Author

Onder, Graziano, primary, Marengoni, Alessandra, additional, Russo, Pierluigi, additional, Degli Esposti, Luca, additional, Fini, Massimo, additional, Monaco, Alessandro, additional, Bonassi, Stefano, additional, Palmer, Katie, additional, Marrocco, Walter, additional, Pozzi, Giuseppe, additional, Sangiorgi, Diego, additional, Buda, Stefano, additional, Marchionni, Niccolò, additional, Mammarella, Federica, additional, Bernabei, Roberto, additional, Pani, Luca, additional, Pecorelli, Sergio, additional, Martinetti, S., additional, Mero, P., additional, Raeli, L., additional, Migliazza, S., additional, Dellagiovanna, M., additional, Cerra, C., additional, Gambera, M., additional, Piccinelli, R., additional, Zambetti, M., additional, Atzeni, F., additional, Valsecchi, V., additional, DeLuca, P., additional, Scopinaro, E., additional, Moltoni, D., additional, Pini, E., additional, Leoni, O., additional, Oria, C., additional, Papagni, M., additional, Nosetti, G., additional, Caldiroli, E., additional, Moser, V., additional, Roni, R., additional, Polverino, A., additional, Bovo, C., additional, Mezzalira, L., additional, Andretta, M., additional, Trentin, L., additional, Palcic, S., additional, Pettinelli, A., additional, Arbo, A., additional, Bertola, A., additional, Capparoni, G., additional, Cattaruzzi, C., additional, Marcuzzo, L., additional, Rosa, F.V., additional, Basso, B., additional, Saglietto, M., additional, Delucis, S., additional, Prioli, M., additional, Filippi, R., additional, Coccini, A., additional, Ghia, M., additional, Sanfelici, F., additional, Radici, S., additional, Scanavacca, P., additional, Campi, A., additional, Bianchi, S., additional, Verzola, A., additional, Morini, M., additional, Borsari, M., additional, Danielli, A., additional, Dal Maso, M., additional, Marsiglia, B., additional, Vujovic, B., additional, Pisani, M., additional, Bonini, P., additional, Lena, F., additional, Aletti, P., additional, Marcobelli, A., additional, Sagratella, S., additional, Fratini, S., additional, Bartolini, F., additional, Riccioni, G., additional, Meneghini, A., additional, Di Turi, R., additional, Fano, V., additional, Blasi, A., additional, Pagnozzi, E., additional, Quintavalle, G., additional, D'Avenia, P., additional, De Matthaeis, M.C., additional, Ferrante, F., additional, Crescenzi, S., additional, Marziale, L., additional, Venditti, P., additional, Bianchi, C., additional, Senesi, I., additional, Baci, R., additional, De Carlo, I., additional, Lavalle, A., additional, Trofa, G., additional, Marcello, G., additional, Pagliaro, C., additional, Troncone, C., additional, Farina, G., additional, Tari, M.G., additional, Motola, G., additional, De Luca, F., additional, Saltarelli, M.L., additional, Granieri, C., additional, Vulnera, M., additional, Palumbo, L., additional, La Viola, F., additional, Florio, L., additional, De Francesco, A.E., additional, Costantino, D., additional, Rapisarda, F., additional, Lazzaro, P.L., additional, Pastorello, M., additional, Parelli, M., additional, Visconti, M., additional, Uomo, I., additional, Sanna, P., additional, and Lombardo, F., additional

Published
2016
Full Text
View/download PDF

31. Trauma-hemorrhagic shock induces a CD36-dependent RBC endothelial-adhesive phenotype

Author

Ghia M. Vinluan, Eleonora Feketeova, Michael R. Condon, Vamsi A. Alli, Zoltán Spolarics, George W. Machiedo, Jordan E. Fishman, Edwin A. Deitch, Leonard Mason, Walter N. Durán, Matthew D. Neal, and Jacquelyn N. Tomaio

Subjects
CD36 Antigens, Male, Pathology, medicine.medical_specialty, Erythrocytes, Endothelium, CD36, Multiple Organ Failure, Adhesion (medicine), In Vitro Techniques, Shock, Hemorrhagic, Critical Care and Intensive Care Medicine, Sensitivity and Specificity, Sampling Studies, Rats, Sprague-Dawley, Random Allocation, Occlusion, medicine, Cell Adhesion, Animals, Humans, Shock, Traumatic, biology, business.industry, medicine.disease, Phenotype, Rats, Disease Models, Animal, medicine.anatomical_structure, Tissue ischemia, Gene Expression Regulation, Hemorrhagic shock, biology.protein, Endothelium, Vascular, business, Multiple organ dysfunction syndrome
Abstract

Microvascular dysfunction is a key element in the development of the multiple organ dysfunction syndrome. Although the mechanisms for this response are unclear, RBC adhesion to endothelium may initiate intravascular occlusion leading to ischemic tissue injury. Thus, we tested the hypothesis that trauma-hemorrhage induces RBC-endothelial cell adhesion.Prospective in vivo and in vitro animal study and analysis of patient blood samples.University research laboratory and hospital emergency and trauma units.We initially assayed RBC adhesion to endothelial cells in vitro using RBCs obtained from rats subjected to trauma-hemorrhagic shock or sham shock as well as from severely injured trauma patients. Subsequently, we measured the role of putative RBCs and endothelial cell receptors in the increased RBC-endothelial cell adhesive response.In both rats and humans, trauma-hemorrhagic shock increased RBC adhesion to endothelium as well as increasing several putative RBC surface adhesion molecules including CD36. The critical factor leading to RBC-endothelial cell adhesion was increased surface RBC CD36 expression. Adhesion of trauma-hemorrhagic shock RBCs was mediated, at least in part, by the binding of RBC CD36 to its cognate endothelial receptors (αVβ3 and VCAM-1). Gut-derived factors carried in the intestinal lymphatics triggered these trauma-hemorrhagic shock-induced RBC changes because 1) preventing trauma-hemorrhagic shock intestinal lymph from reaching the systemic circulation abrogated the RBC effects, 2) in vitro incubation of naïve whole blood with trauma-hemorrhagic shock lymph replicated the in vivo trauma-hemorrhagic shock-induced RBC changes while 3) injection of trauma-hemorrhagic shock lymph into naïve animals recreated the RBC changes observed after actual trauma-hemorrhagic shock.1) Trauma-hemorrhagic shock induces rapid RBC adhesion to endothelial cells in patients and animals. 2) Increased RBC CD36 expression characterizes the RBC-adhesive phenotype. 3) The RBC phenotypic and functional changes were induced by gut-derived humoral factors. These novel findings may explain the microvascular dysfunction occurring after trauma-hemorrhagic shock, sepsis, and other stress states.

Published
2013

35. Le Poste di Mazzoni

Author

Ghia, M

Subjects
Ostia, Angiolo Mazzoni, storia dell'architettura, Lido di Roma
Published
2004

37. Trauma-Hemorrhagic Shock Induces a CD36-Dependent RBC Endothelial-Adhesive Phenotype

Author

Deitch, Edwin A., primary, Condon, Michael, additional, Feketeova, Eleonora, additional, Machiedo, George W., additional, Mason, Leonard, additional, Vinluan, Ghia M., additional, Alli, Vamsi A., additional, Neal, Matthew D., additional, Tomaio, Jacquelyn N., additional, Fishman, Jordan E., additional, Durán, Walter N., additional, and Spolarics, Zoltan, additional

Published
2014
Full Text
View/download PDF

40. Mapping of transcription factor binding regions in mammalian cells by ChIP : comparison of array- and sequencing-based technologies.

Author

Euskirchen, Ghia M, Rozowsky, Joel S, Wei, Chia-Lin, Lee, Wah Heng, Zhang, Zhengdong D, Hartman, Stephen, Emanuelsson, Olof, Stolc, Viktor, Weissman, Sherman, Gerstein, Mark B, Ruan, Yijun, Snyder, Michael, Euskirchen, Ghia M, Rozowsky, Joel S, Wei, Chia-Lin, Lee, Wah Heng, Zhang, Zhengdong D, Hartman, Stephen, Emanuelsson, Olof, Stolc, Viktor, Weissman, Sherman, Gerstein, Mark B, Ruan, Yijun, and Snyder, Michael

Abstract

Recent progress in mapping transcription factor (TF) binding regions can largely be credited to chromatin immunoprecipitation (ChIP) technologies. We compared strategies for mapping TF binding regions in mammalian cells using two different ChIP schemes: ChIP with DNA microarray analysis (ChIP-chip) and ChIP with DNA sequencing (ChIP-PET). We first investigated parameters central to obtaining robust ChIP-chip data sets by analyzing STAT1 targets in the ENCODE regions of the human genome, and then compared ChIP-chip to ChIP-PET. We devised methods for scoring and comparing results among various tiling arrays and examined parameters such as DNA microarray format, oligonucleotide length, hybridization conditions, and the use of competitor Cot-1 DNA. The best performance was achieved with high-density oligonucleotide arrays, oligonucleotides >/=50 bases (b), the presence of competitor Cot-1 DNA and hybridizations conducted in microfluidics stations. When target identification was evaluated as a function of array number, 80%-86% of targets were identified with three or more arrays. Comparison of ChIP-chip with ChIP-PET revealed strong agreement for the highest ranked targets with less overlap for the low ranked targets. With advantages and disadvantages unique to each approach, we found that ChIP-chip and ChIP-PET are frequently complementary in their relative abilities to detect STAT1 targets for the lower ranked targets; each method detected validated targets that were missed by the other method. The most comprehensive list of STAT1 binding regions is obtained by merging results from ChIP-chip and ChIP-sequencing. Overall, this study provides information for robust identification, scoring, and validation of TF targets using ChIP-based technologies.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results