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The emerging high-resolution 3D printing technique called two-photon polymerization (2PP) enables to print devices bottom-up rapidly, contrary to the top-down lithography-based fabrication methods. In this work, various polymer microbeams are 3D printed and their resonant characteristics are analyzed to understand the origin of damping. The 2PP printed polymer resonators have shown less damping than other polymer devices reported earlier, with tensile-stressed clamped-clamped beams reaching a record quality factor of 1819. The resonant energy loss was dominant by bulk friction damping. These results pave the path towards using 3D printed microresonators as mass sensors with improved design and fabrication flexibility., Electronic Instrumentation, Micro and Nano Engineering

Bacteria that are resistant to antibiotics present an increasing burden on healthcare. To address this emerging crisis, novel rapid antibiotic susceptibility testing (AST) methods are eagerly needed. Here, we present an optical AST technique that can determine the bacterial viability within 1 h down to a resolution of single bacteria. The method is based on measuring intensity fluctuations of a reflected laser focused on a bacterium in reflective microwells. Using numerical simulations, we show that both refraction and absorption of light by the bacterium contribute to the observed signal. By administering antibiotics that kill the bacteria, we show that the variance of the detected fluctuations vanishes within 1 h, indicating the potential of this technique for rapid sensing of bacterial antibiotic susceptibility. We envisage the use of this method for massively parallelizable AST tests and fast detection of drug-resistant pathogens., Dynamics of Micro and Nano Systems, Micro and Nano Engineering, BN/Cees Dekker Lab

The capacity to precisely pipette femtoliter volumes of liquid enables many applications, for example, to functionalize a nanoscale surface and manipulate fluids inside a single-cell. A pressure-controlled pipetting method is the most preferred, since it enables the widest range of working liquids. However, precisely controlling femtoliter volumes by pressure is challenging. In this work, a new concept is proposed that makes use of axisymmetrical phaseguides inside a microfluidic channel to pipette liquid in discrete steps of known volume. An analytical model for the design of the femtopipettes is developed and verified experimentally. Femtopipettes are fabricated using a multi-scale 3D printing strategy integrating a digital light processing printed part and a two-photon-polymerization printed part. Three different variants are designed and fabricated with pipetting resolutions of 10 picoliters, 180 femtoliters and 50 femtoliters. As a demonstration, controlled amounts of a water-glycerol mixture were first aspirated and then dispensed into a mineral oil droplet., Electronic Instrumentation, Micro and Nano Engineering

Resonant sensors hold great promise in measuring small masses, to enable future mass spectrometers, and small forces in applications like atomic and magnetic force microscopy. During the last decades, scaling down the size of resonators has led to huge enhancements in sensing resolution, but has also raised the question of what the ultimate limit is. Current knowledge suggests that this limit is reached when a resonator oscillates at the maximum amplitude for which its response is predominantly linear. We present experimental evidence that it is possible to obtain better resolutions by oscillation amplitudes beyond the onset of nonlinearities. An analytical model is developed that explains the observations and unravels the relation between ultimate sensing resolution and speed. In the high-speed limit, we find that the ultimate resolution of a resonator is improved when decreasing its damping. This conclusion contrasts with previous works, which proposed that lowering the damping does not affect or even harms the ultimate sensing resolution., Electronic Instrumentation, Micro and Nano Engineering, Dynamics of Micro and Nano Systems, QN/Groeblacher Lab, QN/Steeneken Lab

Physical patterns represent potential surface cues for promoting osteogenic differentiation of stem cells and improving osseointegration of orthopedic implants. Understanding the early cell–surface interactions and their effects on late cellular functions is essential for a rational design of such topographies, yet still elusive. In this work, fluidic force microscopy (FluidFM) and atomic force microscopy (AFM) combined with optical and electron microscopy are used to quantitatively investigate the interaction of preosteoblasts with 3D-printed patterns after 4 and 24 h of culture. The patterns consist of pillars with the same diameter (200 nm) and interspace (700 nm) but distinct heights (500 and 1000 nm) and osteogenic properties. FluidFM reveals a higher cell adhesion strength after 24 h of culture on the taller pillars (32 ± 7 kPa versus 21.5 ± 12.5 kPa). This is associated with attachment of cells partly on the sidewalls of these pillars, thus requiring larger normal forces for detachment. Furthermore, the higher resistance to shear forces observed for these cells indicates an enhanced anchorage and can be related to the persistence and stability of lamellipodia. The study explains the differential cell adhesion behavior induced by different pillar heights, enabling advancements in the rational design of osteogenic patterns., Micro and Nano Engineering, Biomaterials & Tissue Biomechanics

Microfluidic organs-on-chips (OoCs) technology has emerged as the trend for in vitro functional modeling of organs in recent years. Simplifying the complexities of the human organs under controlled perfusion of required fluids paves the way for accurate prediction of human organ functionalities and their response to interventions like exposure to drugs. However, in the state-of-the-art OoC, the existing methods to control fluids use external bulky peripheral components and systems much larger than the chips used in experiments. A new generation of compact microfluidic flow control systems is needed to overcome this challenge. This study first presents a structured classification of OoC devices according to their types and microfluidic complexities. Next, we suggest three fundamental fluid flow control mechanisms and define component configurations for different levels of OoC complexity for each respective mechanism. Finally, we propose an architecture integrating modular microfluidic flow control components and OoC devices on a single platform. We emphasize the need for miniaturization of flow control components to achieve portability, minimize sample usage, minimize dead volume, improve the flowing time of fluids to the OoC cell chamber, and enable long-duration experiments., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Micro and Nano Engineering, Precision and Microsystems Engineering

The use of nanoparticles has been growing in various industrial fields, and concerns about their effects on health and the environment have been increasing. Hence, characterization techniques for nanoparticles are essential. Here, we present a silicon dioxide microfabricated suspended microchannel resonator (SMR) to measure the mass and concentration of nanoparticles in a liquid as they flow. We measured the mass detection limits of the device using laser Doppler vibrometry. This limit reached a minimum of 377 ag that correspond to a 34 nm diameter gold nanoparticle or a 243 nm diameter polystyrene particle, when sampled every 30 ms. We compared the fundamental limits of the measured data with an ideal noiseless measurement of the SMR. Finally, we measured the buoyant mass of gold nanoparticles in real-time as they flowed through the SMR. [Figure not available: see fulltext.]., Electronic Instrumentation, Micro and Nano Engineering, Electronic Components, Technology and Materials

The surface topography of implantable devices is of crucial importance for guiding the cascade of events that starts from the initial contact of the cells with the surface and continues until the complete integration of the device in its immediate environment. There is, however, limited quantitative information available regarding the relationships between the different stages of such cascade(s) and how the design of surface topography influences them. We, therefore, used direct laser writing to 3D-print submicron pillars with precisely controlled dimensions and spatial arrangements to perform a systematic study of such relationships. Using single-cell force spectroscopy, we measured the adhesion force and the work of adhesion of the preosteoblast cells residing on the different types of surfaces. Not only the adhesion parameters (after 2-60 s) but also the formation of focal adhesions was strongly dependent on the geometry and arrangement of the pillars: sufficiently tall and dense pillars enhanced both adhesion parameters and the formation of focal adhesions. Our morphological study of the cells (after 24 h) showed that those enhancements were associated with a specific way of cell settlement onto the surface (i.e., "top state"). The cells interacting with tall and dense pillars were also characterized by numerous thick actin stress fibers in the perinuclear region and possibly high internal stresses. Furthermore, living cells with highly organized cytoskeletal networks exhibited greater values of the elastic modulus. The early responses of the cells predicted their late response including matrix mineralization: tall and dense submicron pillars significantly upregulated the expression of osteopontin after 21 days of culture under both osteogenic and nonosteogenic conditions. Our findings paint a detailed picture of at least one possible cascade of events that starts from initial cell adhesion and continues to subsequent cellular functions and eventual matrix mi, Biomaterials & Tissue Biomechanics, Micro and Nano Engineering, ChemE/Product and Process Engineering

Despite the potential of small-scale pillars of black titanium (bTi) for killing the bacteria and directing the fate of stem cells, not much is known about the effects of the pillars’ design parameters on their biological properties. Here, three distinct bTi surfaces are designed and fabricated through dry etching of the titanium, each featuring different pillar designs. The interactions of the surfaces with MC3T3-E1 preosteoblast cells and Staphylococcus aureus bacteria are then investigated. Pillars with different heights and spatial organizations differently influence the morphological characteristics of the cells, including their spreading area, aspect ratio, nucleus area, and cytoskeletal organization. The preferential formation of focal adhesions (FAs) and their size variations also depend on the type of topography. When the pillars are neither fully separated nor extremely tall, the colocalization of actin fibers and FAs as well as an enhanced matrix mineralization are observed. However, the killing efficiency of these pillars against the bacteria is not as high as that of fully separated and tall pillars. This study provides a new perspective on the dual-functionality of bTi surfaces and elucidates how the surface design and fabrication parameters can be used to achieve a surface topography with balanced bactericidal and osteogenic properties., Biomaterials & Tissue Biomechanics, Micro and Nano Engineering, BT/Biocatalysis

Micro- and nano-patterns are gaining increasing attraction in several fields ranging from nanoelectronics to bioengineering. The mechanical properties of the nanostructures (nanopillars, nanotubes, nanowires, etc.) are highly relevant for many applications but challenging to determine. Existing mechanical characterization methods require mounting the testing setup inside a scanning electron microscope (SEM) and additional sample modification. Here, we propose two atomic force microscopy (AFM) methods, based on contact mode imaging (CMI) and force spectroscopy imaging (FSI), to determine the mechanical characteristics of individual micro- and nanopillars as fabricated, without using SEM. We present the working principles of both methods and two case studies on nanopillars fabricated by additive manufacturing methods: two-photon polymerization (2PP) and electron beam induced deposition (EBID). Various mechanical parameters were determined using CMI and FSI, respectively. For the 2PP nanopillars, we measured the stiffness (13.5 ± 3.2 N/m and 15.9 ± 2.6 N/m), the maximum lateral force (883.0 ± 89.5 nN and 889.6 ± 113.6 nN), the maximum deflection (64.2 ± 13.6 nm and 58.3 ± 14.24 nm), the failure stress (0.3 ± 0.03 GPa and 0.3 ± 0.02 GPa), and the adhesion force (56.6 ± 4.5 µN and 58.6 ± 5.2 µN). For the EBID nanopillars, we measured the failure stress (2.9 ± 0.2 GPa and 2.7 ± 0.4 GPa). The similar results obtained using both techniques confirmed the efficacy and consistency of the methods. The proposed methodologies have the potential of enabling otherwise impossible measurements particularly when the specimens need to be tested under wet conditions, such as patterns for mechanobiological studies., Micro and Nano Engineering, Biomaterials & Tissue Biomechanics, ImPhys/Microscopy Instrumentation & Techniques

The surface topography of implantable devices is of crucial importance for guiding the cascade of events that starts from the initial contact of the cells with the surface and continues until the complete integration of the device in its immediate environment. There is, however, limited quantitative information available regarding the relationships between the different stages of such cascade(s) and how the design of surface topography influences them. We, therefore, used direct laser writing to 3D-print submicron pillars with precisely controlled dimensions and spatial arrangements to perform a systematic study of such relationships. Using single-cell force spectroscopy, we measured the adhesion force and the work of adhesion of the preosteoblast cells residing on the different types of surfaces. Not only the adhesion parameters (after 2-60 s) but also the formation of focal adhesions was strongly dependent on the geometry and arrangement of the pillars: sufficiently tall and dense pillars enhanced both adhesion parameters and the formation of focal adhesions. Our morphological study of the cells (after 24 h) showed that those enhancements were associated with a specific way of cell settlement onto the surface (i.e., "top state"). The cells interacting with tall and dense pillars were also characterized by numerous thick actin stress fibers in the perinuclear region and possibly high internal stresses. Furthermore, living cells with highly organized cytoskeletal networks exhibited greater values of the elastic modulus. The early responses of the cells predicted their late response including matrix mineralization: tall and dense submicron pillars significantly upregulated the expression of osteopontin after 21 days of culture under both osteogenic and nonosteogenic conditions. Our findings paint a detailed picture of at least one possible cascade of events that starts from initial cell adhesion and continues to subsequent cellular functions and eventual matrix mi, Biomaterials & Tissue Biomechanics, Micro and Nano Engineering, ChemE/Product and Process Engineering

Despite the potential of small-scale pillars of black titanium (bTi) for killing the bacteria and directing the fate of stem cells, not much is known about the effects of the pillars’ design parameters on their biological properties. Here, three distinct bTi surfaces are designed and fabricated through dry etching of the titanium, each featuring different pillar designs. The interactions of the surfaces with MC3T3-E1 preosteoblast cells and Staphylococcus aureus bacteria are then investigated. Pillars with different heights and spatial organizations differently influence the morphological characteristics of the cells, including their spreading area, aspect ratio, nucleus area, and cytoskeletal organization. The preferential formation of focal adhesions (FAs) and their size variations also depend on the type of topography. When the pillars are neither fully separated nor extremely tall, the colocalization of actin fibers and FAs as well as an enhanced matrix mineralization are observed. However, the killing efficiency of these pillars against the bacteria is not as high as that of fully separated and tall pillars. This study provides a new perspective on the dual-functionality of bTi surfaces and elucidates how the surface design and fabrication parameters can be used to achieve a surface topography with balanced bactericidal and osteogenic properties., Biomaterials & Tissue Biomechanics, Micro and Nano Engineering, BT/Biocatalysis

Micro- and nano-patterns are gaining increasing attraction in several fields ranging from nanoelectronics to bioengineering. The mechanical properties of the nanostructures (nanopillars, nanotubes, nanowires, etc.) are highly relevant for many applications but challenging to determine. Existing mechanical characterization methods require mounting the testing setup inside a scanning electron microscope (SEM) and additional sample modification. Here, we propose two atomic force microscopy (AFM) methods, based on contact mode imaging (CMI) and force spectroscopy imaging (FSI), to determine the mechanical characteristics of individual micro- and nanopillars as fabricated, without using SEM. We present the working principles of both methods and two case studies on nanopillars fabricated by additive manufacturing methods: two-photon polymerization (2PP) and electron beam induced deposition (EBID). Various mechanical parameters were determined using CMI and FSI, respectively. For the 2PP nanopillars, we measured the stiffness (13.5 ± 3.2 N/m and 15.9 ± 2.6 N/m), the maximum lateral force (883.0 ± 89.5 nN and 889.6 ± 113.6 nN), the maximum deflection (64.2 ± 13.6 nm and 58.3 ± 14.24 nm), the failure stress (0.3 ± 0.03 GPa and 0.3 ± 0.02 GPa), and the adhesion force (56.6 ± 4.5 µN and 58.6 ± 5.2 µN). For the EBID nanopillars, we measured the failure stress (2.9 ± 0.2 GPa and 2.7 ± 0.4 GPa). The similar results obtained using both techniques confirmed the efficacy and consistency of the methods. The proposed methodologies have the potential of enabling otherwise impossible measurements particularly when the specimens need to be tested under wet conditions, such as patterns for mechanobiological studies., Micro and Nano Engineering, Biomaterials & Tissue Biomechanics, ImPhys/Microscopy Instrumentation & Techniques

Surfactant losses by adsorption to rock surfaces make surfactant-based enhanced oil recovery economically less feasible. We investigated polyacrylate (PA) as a sacrificial agent in the reduction of anionic surfactant adsorption with focus on calcite surfaces by using quartz crystal microbalance with dissipation monitoring. It was found that the adsorption of the anionic surfactant alcohol alkoxy sulfate (AAS) followed a Langmuir adsorption isotherm, and the adsorbed amount reached saturation above its critical micellar concentration. Adsorption of PA was a much slower process compared to AAS adsorption. Increasing the calcium ion concentration also increased the amount of AAS adsorbed as well as the mass increase rate of PA adsorption. Experimental results combined with density functional theory calculations indicated that calcium cation bridging was important for anionic surfactant AAS and PA adsorption to calcite surfaces. To effectively reduce the amount of surfactant adsorption, it was needed to preflush with PA, rather than by a simultaneous injection. Preflushing with 30 ppm of PA gave a reduction of AAS adsorption of 30% under high salinity (HS, 31,800 ppm) conditions, compared to 8% reduction under low salinity (LS, 3180 ppm) conditions. In the absence of PA, the amount of adsorbed AAS was reduced by already 50% upon changing from HS to LS conditions. Lower calcium ion concentrations, as under LS conditions, contributed to this observation. On different mineral surfaces, PA reduced the AAS adsorption in the order of alumina > calcite > silica. These results offer important insights into mitigating surfactant adsorption using PA polyelectrolyte as sacrificial agent and contribute to improved flooding strategies with reduced surfactant loss., ChemE/Advanced Soft Matter, OLD ChemE/Organic Materials and Interfaces, Micro and Nano Engineering

Functional gradients are material transitions that are found in nature and are known to result in materials with superior properties and multiple functionalities. The emerging multi-material 3D printing (=additive manufacturing, AM) techniques provide a powerful tool for the design and fabrication of bioinspired functionally graded materials (FGMs). In particular, the spatial distribution of materials can be controlled at the level of individual volumetric pixels (voxels i.e., cubes with side lengths of 20–40 μm), thereby ensuring accuracy, reliability, and reproducibility of the obtained properties and allowing for systematic studies of how various design variables affect the deformation and fracture behaviors of FGMs. Here, we designed, 3D printed, and mechanically tested tensile and notched FGMs specimens with step-wise (i.e., 5-, 10-, and 15-steps) and continuous (sigmoid and linear) gradients. The deformation and fracture mechanisms of these FGM composites were studied using digital image correlation, digital microscopy, and scanning electron microscopy. We further characterized the chemical composition and local mechanical properties of FGM composites using XPS and nanoindentation measurements, respectively. Tensile test specimens with a continuous gradient (i.e., linear) exhibited much higher Young's moduli (≈3-folds) and ultimate strengths (≈2-folds) but lower elongations (≈2-folds drop) as compared to those with stepwise gradients (i.e., 5-steps). Similarly, notched specimens with linear gradients exhibited 2-folds higher values of the stiffness and fracture stress, but 1.5-folds lower fracture strains as compared to those with 5-steps gradients. Although we found non-uniform highly concentrated strain distributions in all specimens, FGMs with linear gradients showed a smoother strain distribution and smaller crack blunting zones as compared to those with stepwise gradients. Our results imply that for stiffness and strength linear-gradient perform better, Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Biomaterials & Tissue Biomechanics, Micro and Nano Engineering, Mechatronic Design

Microfluidic atomic force microscopy (AFM) cantilever probes have all the functionalities of a standard AFM cantilever along with fluid pipetting. They have a channel inside the cantilever and an aperture at the tip. Such probes are useful for precise fluid manipulation at a desired location, for example near or inside cells. They are typically made by complex microfabrication process steps, resulting in expensive probes. Here, we used two different 3D additive manufacturing techniques, stereolithography and two-photon polymerization, to directly print ready-to-use microfluidic AFM cantilever probes. This approach has considerably reduced the fabrication time and increased the design freedom. One of the probes, 564 μm long, 30 μm wide, 30 μm high, with a 25 μm diameter channel and 2.5 μm wall thickness had a spring constant of 3.7 N m-1 and the polymer fabrication material had an elastic modulus of 4.2 GPa. Using these 3D printed probes, AFM imaging of a surface, puncturing of the cell membrane, and aspiration at the single cell level have been demonstrated., Micro and Nano Engineering, Biomaterials & Tissue Biomechanics

Fabricating large areas of geometrically complex and precisely controlled topographies is required for the studies of cell behavior on patterned surfaces. Direct laser writing (DLW) is an advanced 3D-fabrication technique, which facilitates the manufacturing of structures within various scales (from a few hundred nanometers to millimeters). However, this method requires improvements in the accuracy and reproducibility of the submicron and nanoscale features that are printed over a large area. Here, we present a scheme to both improve the uniformity of the printed submicron patterns and decrease the printing time. The effects of various processing parameters (e.g., laser power and writing field) on the dimensions and uniformity of submicron pillars as well as on their Young’s modulus and surface wettability were assessed. Decreasing the writing field to 33 × 33 μm2 significantly improved the uniformity of submicron pillars that were printed over an area of 4 mm2 in a single-step process. Preosteoblast cells (MC3T3-E1) were used to assess the cytocompatibility of the used material (IP-L780 resin) with a focus on cell morphology, cell proliferation, cytoskeletal organization, and the elastic modulus of the cells. The cells cultured for 2 days on the submicron pillars showed a polarized shape and a higher Young’s modulus of the area corresponding to the nucleus relative to those cultured on flat surfaces. Taken together, the results of the current study clearly show that the submicron patterns created using DLW are both cytocompatible and could modulate the morphology and mechanical properties of cells. This work paves the way for direct printing of submicron features with controlled Young’s moduli over large areas in a single-step process, which is necessary for systematically studying how such patterns modulate cellular functions., Biomaterials & Tissue Biomechanics, Micro and Nano Engineering

Resonant sensors determine a sensed parameter by measuring the resonance frequency of a resonator. For fast continuous sensing, it is desirable to operate resonant sensors in a closed-loop configuration, where a feedback loop ensures that the resonator is always actuated near its resonance frequency, so that the precision is maximized even in the presence of drifts or fluctuations of the resonance frequency. However, in a closed-loop configuration, the precision is not only determined by the resonator itself, but also by the feedback loop, even if the feedback circuit is noiseless. Therefore, to characterize the intrinsic precision of resonant sensors, the open-loop configuration is often employed. To link these measurements to the actual closed-loop performance of the resonator, it is desirable to have a relation that determines the closed-loop precision of the resonator from open-loop characterisation data. In this work, we present a methodology to estimate the closed-loop resonant sensor precision by relying only on an open-loop characterization of the resonator. The procedure is beneficial for fast performance estimation and benchmarking of resonant sensors, because it does not require actual closed-loop sensor operation, thus being independent on the particular implementation of the feedback loop. We validate the methodology experimentally by determining the closed-loop precision of a mechanical resonator from an open-loop measurement and comparing this to an actual closed-loop measurement., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Dynamics of Micro and Nano Systems, QN/Steeneken Lab, Micro and Nano Engineering

We introduce a two-channel microfluidic atomic force microscopy (AFM) cantilever that combines the nanomechanical sensing functionality of an AFM cantilever with the ability to manipulate fluids of picolitres or smaller volumes through nanoscale apertures near the cantilever tip. Each channel is connected to a separate fluid reservoir, which can be independently controlled by pressure. Various systematic experiments with fluorescent liquids were done by either injecting the liquids from the on-chip reservoir or aspirating directly through the nanoscale apertures at the tip. A flow rate analysis of volume dosing, aspiration and concentration dosing inside the liquid medium was performed. To understand the fluid behaviour, an analytical model based on the hydrodynamic resistance, as well as numerical flow simulations of single and multi-phase conditions were performed and compared. By applying pressures between -500 mbar and 500 mbar to the reservoirs of the probe with respect to the ambient pressure, flow rates ranging from 10 fl s-1 to 83 pl s-1 were obtained inside the channels of the cantilever as predicted by the analytical model. The smallest dosing flow rate through the apertures was 720 fl s-1, which was obtained with a 10 mbar pressure on one reservoir and ambient pressure on the other. The solute concentration in the outflow could be tuned to values between 0% and 100% by pure convection and to values between 17.5% and 90% in combination with diffusion. The results prove that this new probe enables handling multiple fluids with the scope to inject different concentrations of analytes inside a single living cell and also perform regular AFM functionalities., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Micro and Nano Engineering, BN/Bionanoscience

One of the methods to create sub-10 nm resolution metal-composed 3D nanopillars is electron beam-induced deposition (EBID). Surface nanotopographies (e.g., nanopillars) could play an important role in the design and fabrication of implantable medical devices by preventing the infections that are caused by the bacterial colonization of the implant surface. The mechanical properties of such nanoscale structures can influence their bactericidal efficiency. In addition, these properties are key factors in determining the fate of stem cells. In this study, we quantified the relevant mechanical properties of EBID nanopillars interacting with Staphylococcus aureus (S. aureus) using atomic force microscopy (AFM). We first determined the elastic modulus (17.7 GPa) and the fracture stress (3.0 ± 0.3 GPa) of the nanopillars using the quantitative imaging (QI) mode and contact mode (CM) of AFM. The displacement of the nanopillars interacting with the bacteria cells was measured by scanning electron microscopy (50.3 ± 9.0 nm). Finite element method based simulations were then applied to obtain the force-displacement curve of the nanopillars (considering the specified dimensions and the measured value of the elastic modulus) based on which an interaction force of 88.7 ± 36.1 nN was determined. The maximum von Mises stress of the nanopillars subjected to these forces was also determined (3.2 ± 0.3 GPa). These values were close to the maximum (i.e., fracture) stress of the pillars as measured by AFM, indicating that the nanopillars were close to their breaking point while interacting with S. aureus. These findings reveal unique quantitative data regarding the mechanical properties of nanopillars interacting with bacterial cells and highlight the possibilities of enhancing the bactericidal activity of the investigated EBID nanopillars by adjusting both their geometry and mechanical properties., Biomaterials & Tissue Biomechanics, Micro and Nano Engineering, ImPhys/Microscopy Instrumentation & Techniques, BT/Biocatalysis

Suspended microfluidic resonators enable detection of fluid density and viscosity with high sensitivity. Here, a two-legged suspended microchannel resonator that probes pico-litres of liquid is presented. The higher resonant modes (flexural and torsional) were explored for increased sensitivity and resolution. Unlike other reported microchannel resonators, this device showed an increase in the quality factor with resonant frequency value. The performance of the resonator was tested by filling the channel with three liquids, one at a time, over a density range of 779 - 1110 Kg/m^3 and a viscosity range of 0.89 - 16.2 mPa.s. The highest resolution obtained was 0.011% change in density. Measurements with torsional mode showed an improvement of about six times in sensitivity and about fifteen times in resolution compared to the first flexural mode. When the empty channel was filled with liquids of different viscosity, the quality factor of the first flexural mode remained overall constant with a variation below 3.3% between the fluids, and confirming the inherent property of suspended microchannel resonators. However, it significantly decreased for second flexural and torsional modes. No noticeable difference was observed in the quality factor between different liquid viscosities for all modes., Dynamics of Micro and Nano Systems, Micro and Nano Engineering, Structural Optimization and Mechanics, EKL Processing

Recent progress in nano-/micro-fabrication techniques has paved the way for the emergence of synthetic bactericidal patterned surfaces that are capable of killing the bacteria via mechanical mechanisms. Different design parameters are known to affect the bactericidal activity of nanopatterns. Evaluating the effects of each parameter, isolated from the others, requires systematic studies. Here, we systematically assessed the effects of the interspacing and disordered arrangement of nanopillars on the bactericidal properties of nanopatterned surfaces. Electron beam induced deposition (EBID) was used to additively manufacture nanopatterns with precisely controlled dimensions (i.e., a height of 190 nm, a diameter of 80 nm, and interspaces of 100, 170, 300, and 500 nm) as well as disordered versions of them. The killing efficiency of the nanopatterns against Gram-positive Staphylococcus aureus bacteria increased by decreasing the interspace, achieving the highest efficiency of 62 ± 23% on the nanopatterns with 100 nm interspacing. By comparison, the disordered nanopatterns did not influence the killing efficiency significantly, as compared to their ordered correspondents. Direct penetration of nanopatterns into the bacterial cell wall was identified as the killing mechanism according to cross-sectional views, which is consistent with previous studies. The findings indicate that future studies aimed at optimizing the design of nanopatterns should focus on the interspacing as an important parameter affecting the bactericidal properties. In combination with controlled disorder, nanopatterns with contrary effects on bacterial and mammalian cells may be developed, Biomaterials & Tissue Biomechanics, ImPhys/Microscopy Instrumentation & Techniques, BT/Biocatalysis, Micro and Nano Engineering

Resonant sensors determine a sensed parameter by measuring the resonance frequency of a resonator. For fast continuous sensing, it is desirable to operate resonant sensors in a closed-loop configuration, where a feedback loop ensures that the resonator is always actuated near its resonance frequency, so that the precision is maximized even in the presence of drifts or fluctuations of the resonance frequency. However, in a closed-loop configuration, the precision is not only determined by the resonator itself, but also by the feedback loop, even if the feedback circuit is noiseless. Therefore, to characterize the intrinsic precision of resonant sensors, the open-loop configuration is often employed. To link these measurements to the actual closed-loop performance of the resonator, it is desirable to have a relation that determines the closed-loop precision of the resonator from open-loop characterisation data. In this work, we present a methodology to estimate the closed-loop resonant sensor precision by relying only on an open-loop characterization of the resonator. The procedure is beneficial for fast performance estimation and benchmarking of resonant sensors, because it does not require actual closed-loop sensor operation, thus being independent on the particular implementation of the feedback loop. We validate the methodology experimentally by determining the closed-loop precision of a mechanical resonator from an open-loop measurement and comparing this to an actual closed-loop measurement., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Dynamics of Micro and Nano Systems, QN/Steeneken Lab, Micro and Nano Engineering

We introduce a two-channel microfluidic atomic force microscopy (AFM) cantilever that combines the nanomechanical sensing functionality of an AFM cantilever with the ability to manipulate fluids of picolitres or smaller volumes through nanoscale apertures near the cantilever tip. Each channel is connected to a separate fluid reservoir, which can be independently controlled by pressure. Various systematic experiments with fluorescent liquids were done by either injecting the liquids from the on-chip reservoir or aspirating directly through the nanoscale apertures at the tip. A flow rate analysis of volume dosing, aspiration and concentration dosing inside the liquid medium was performed. To understand the fluid behaviour, an analytical model based on the hydrodynamic resistance, as well as numerical flow simulations of single and multi-phase conditions were performed and compared. By applying pressures between -500 mbar and 500 mbar to the reservoirs of the probe with respect to the ambient pressure, flow rates ranging from 10 fl s-1 to 83 pl s-1 were obtained inside the channels of the cantilever as predicted by the analytical model. The smallest dosing flow rate through the apertures was 720 fl s-1, which was obtained with a 10 mbar pressure on one reservoir and ambient pressure on the other. The solute concentration in the outflow could be tuned to values between 0% and 100% by pure convection and to values between 17.5% and 90% in combination with diffusion. The results prove that this new probe enables handling multiple fluids with the scope to inject different concentrations of analytes inside a single living cell and also perform regular AFM functionalities., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Micro and Nano Engineering, BN/Bionanoscience

One of the methods to create sub-10 nm resolution metal-composed 3D nanopillars is electron beam-induced deposition (EBID). Surface nanotopographies (e.g., nanopillars) could play an important role in the design and fabrication of implantable medical devices by preventing the infections that are caused by the bacterial colonization of the implant surface. The mechanical properties of such nanoscale structures can influence their bactericidal efficiency. In addition, these properties are key factors in determining the fate of stem cells. In this study, we quantified the relevant mechanical properties of EBID nanopillars interacting with Staphylococcus aureus (S. aureus) using atomic force microscopy (AFM). We first determined the elastic modulus (17.7 GPa) and the fracture stress (3.0 ± 0.3 GPa) of the nanopillars using the quantitative imaging (QI) mode and contact mode (CM) of AFM. The displacement of the nanopillars interacting with the bacteria cells was measured by scanning electron microscopy (50.3 ± 9.0 nm). Finite element method based simulations were then applied to obtain the force-displacement curve of the nanopillars (considering the specified dimensions and the measured value of the elastic modulus) based on which an interaction force of 88.7 ± 36.1 nN was determined. The maximum von Mises stress of the nanopillars subjected to these forces was also determined (3.2 ± 0.3 GPa). These values were close to the maximum (i.e., fracture) stress of the pillars as measured by AFM, indicating that the nanopillars were close to their breaking point while interacting with S. aureus. These findings reveal unique quantitative data regarding the mechanical properties of nanopillars interacting with bacterial cells and highlight the possibilities of enhancing the bactericidal activity of the investigated EBID nanopillars by adjusting both their geometry and mechanical properties., Biomaterials & Tissue Biomechanics, Micro and Nano Engineering, ImPhys/Microscopy Instrumentation & Techniques, BT/Biocatalysis

Recent progress in nano-/micro-fabrication techniques has paved the way for the emergence of synthetic bactericidal patterned surfaces that are capable of killing the bacteria via mechanical mechanisms. Different design parameters are known to affect the bactericidal activity of nanopatterns. Evaluating the effects of each parameter, isolated from the others, requires systematic studies. Here, we systematically assessed the effects of the interspacing and disordered arrangement of nanopillars on the bactericidal properties of nanopatterned surfaces. Electron beam induced deposition (EBID) was used to additively manufacture nanopatterns with precisely controlled dimensions (i.e., a height of 190 nm, a diameter of 80 nm, and interspaces of 100, 170, 300, and 500 nm) as well as disordered versions of them. The killing efficiency of the nanopatterns against Gram-positive Staphylococcus aureus bacteria increased by decreasing the interspace, achieving the highest efficiency of 62 ± 23% on the nanopatterns with 100 nm interspacing. By comparison, the disordered nanopatterns did not influence the killing efficiency significantly, as compared to their ordered correspondents. Direct penetration of nanopatterns into the bacterial cell wall was identified as the killing mechanism according to cross-sectional views, which is consistent with previous studies. The findings indicate that future studies aimed at optimizing the design of nanopatterns should focus on the interspacing as an important parameter affecting the bactericidal properties. In combination with controlled disorder, nanopatterns with contrary effects on bacterial and mammalian cells may be developed, Biomaterials & Tissue Biomechanics, ImPhys/Microscopy Instrumentation & Techniques, BT/Biocatalysis, Micro and Nano Engineering

Suspended microfluidic resonators enable detection of fluid density and viscosity with high sensitivity. Here, a two-legged suspended microchannel resonator that probes pico-litres of liquid is presented. The higher resonant modes (flexural and torsional) were explored for increased sensitivity and resolution. Unlike other reported microchannel resonators, this device showed an increase in the quality factor with resonant frequency value. The performance of the resonator was tested by filling the channel with three liquids, one at a time, over a density range of 779 - 1110 Kg/m^3 and a viscosity range of 0.89 - 16.2 mPa.s. The highest resolution obtained was 0.011% change in density. Measurements with torsional mode showed an improvement of about six times in sensitivity and about fifteen times in resolution compared to the first flexural mode. When the empty channel was filled with liquids of different viscosity, the quality factor of the first flexural mode remained overall constant with a variation below 3.3% between the fluids, and confirming the inherent property of suspended microchannel resonators. However, it significantly decreased for second flexural and torsional modes. No noticeable difference was observed in the quality factor between different liquid viscosities for all modes., Dynamics of Micro and Nano Systems, Micro and Nano Engineering, Computational Design and Mechanics, EKL Processing

Microvalves are important flow-control devices in many standalone and integrated microfluidic applications. Polydimethylsiloxane (PDMS)-based pneumatic microvalves are commonly used but they generally require large peripheral connections that decrease portability. There are many alternatives found in the literature that use Si-based microvalves, but variants that can throttle even moderate pressures (1) tend to be bulky (cm-range) or consume high power. This paper details the development of a low-power, normally-open piezoelectric microvalve to control flows with a maximum driving pressure of 1, but also retain a small effective form-factor of 5x5x1.8. A novel combination of rapid prototyping methods like stereolithography and laser-cutting have been used to realize this device. The maximum displacement of the fabricated piezoelectric microactuator was measured to be 8.5 at 150. The fabricated microvalve has a flow range of 0–90 at 1 inlet pressure. When fully closed, a leakage of 0.8 open-flow was observed with a power-consumption of 37.5. A flow resolution of 0.2— De-ionized (DI) water was measured at 0.5 pressure., Micro and Nano Engineering

Microvalves are important flow-control components in many standalone and integrated microfluidic circuits. Although there is a large body of work regarding microvalves, there is still a need for an easyto-fabricate, small-footprint, low-power device that can control both liquids and gases at moderate pressures. This paper details the development of a piezoelectric microvalve compatible with both liquids and gases with a maximum driving pressure of 1 bar. A novel combination of accessible methods like 3D-printing and lasercutting has been used to realize this device. The device has a flow range of 0 - 90 μL min−1 at 1 bar inlet pressure. When fully closed, a leakage of 0.8% open-flow was measured with a power consumption of 37.5 μW., Micro and Nano Engineering

Diamond is a highly desirable material for state-of-the-art micro-electromechanical (MEMS) devices, radio-frequency filters and mass sensors, due to its extreme properties and robustness. However, the fabrication/integration of diamond structures into Si-based components remain costly and complex. In this work, a lithography-free, low-cost method is introduced to fabricate diamond-based micro-resonators: a modified home/office desktop inkjet printer is used to locally deposit nanodiamond ink as ∅50–60 µm spots, which are grown into ≈1 µm thick nanocrystalline diamond film disks by chemical vapor deposition, and suspended by reactive ion etching. The frequency response of the fabricated structures is analyzed by laser interferometry, showing resonance frequencies in the range of ≈9–30 MHz, with Q-factors exceeding 104, and (f0 × Q) figure of merit up to ≈2.5 × 1011 Hz in vacuum. Analysis in controlled atmospheres shows a clear dependence of the Q-factors on gas pressure up until 1 atm, with Q ∝ 1/P. When applied as mass sensors, the inkjet-printed diamond resonators yield mass responsivities up to 981 Hz fg−1 after Au deposition, and ultrahigh mass resolution up to 278 ± 48 zg, thus outperforming many similar devices produced by traditional top-down, lithography-based techniques. In summary, this work demonstrates the fabrication of functional high-performance diamond-based micro-sensors by direct inkjet printing., Micro and Nano Engineering, Dynamics of Micro and Nano Systems, QN/Steeneken Lab

Surfactants have the ability to mobilize residual oil trapped in pore spaces of matrix rocks by lowering the oil-water interfacial tension, resulting in a higher oil recovery. However, the loss of surfactants by adsorption onto the rock surface has become a major concern that reduces the efficiency of the surfactant flooding process. In this study, the adsorption behavior of an anionic surfactant to a clay mineral surface was investigated by quartz crystal microbalance with dissipation monitoring upon variations with different cation conditions. Through recording the change of frequency and dissipation of clay-modified sensors, it allows us to do a real-time quantitative analysis of the surfactant adsorption with nanogram sensitivity. The results revealed that the surfactant adsorption increased in a Ca2+-containing solution with increasing pH from 6 to 11, whereas from a Na+-containing solution, more adsorption occurred at acidic conditions. The adsorbed amount went through a maximum (∼200 mM) as a function of the Ca2+ concentration, and the Voigt model suggested that multilayer adsorption of surfactants could be as many as 4-6 monolayers. Using mixed cation (Ca2+ and Na+) solutions, the amount of adsorbed surfactant decreased linearly with decreasing fraction of CaCl2, but Na+ competed for about ∼30% adsorption sites. The importance of the presence of CaCl2 for the surfactant adsorption was stressed in high-salinity and low-salinity solutions in the presence and absence of Ca2+. Furthermore, increasing the temperature from 23 to 65 °C shows first a small increase of surfactant adsorption followed by a reduction of about 20%. The obtained results contribute to a better understanding of surfactant adsorption on clay surfaces and a guide to optimal flooding conditions with reduced surfactant loss., Green Open Access added to TU Delft Institutional Repository'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., OLD ChemE/Organic Materials and Interfaces, Micro and Nano Engineering

Current wafer-scale fabrication methods for graphene-based electronics and sensors involve the transfer of single-layer graphene by a support polymer. This often leaves some polymer residue on the graphene, which can strongly impact its electronic, thermal, and mechanical resonance properties. To assess the cleanliness of graphene fabrication methods, it is thus of considerable interest to quantify the amount of contamination on top of the graphene. Here, we present a methodology for the direct measurement of the mass of the graphene sheet using quartz crystal microbalances (QCMs). By monitoring the QCM resonance frequency during removal of graphene in an oxygen plasma, the total mass of the graphene and contamination is determined with sub-graphene-monolayer accuracy. Since the etch-rate of the contamination is higher than that of graphene, quantitative measurements of the mass of contaminants below, on top, and between graphene layers are obtained. We find that polymer-based dry transfer methods can increase the mass of a graphene sheet by a factor of 10. The presented mass measurement method is conceptually straightforward to interpret and can be used for standardized testing of graphene transfer procedures in order to improve the quality of graphene devices in future applications., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., QN/Steeneken Lab, Electronic Components, Technology and Materials, QN/van der Zant Lab, Dynamics of Micro and Nano Systems, Micro and Nano Engineering

An experimental setup to perform dynamic analysis of a micro- and nano-mechanical system in vacuum, gas, and liquid is presented. The setup mainly consists of a piezoelectric excitation part and the chamber that can be either evacuated for vacuum, or filled with gas or water. The design of the piezoelectric actuator was based on a Langevin transducer. The chamber is made out of materials that can sustain: vacuum, variety of gases and different types of liquids (mild acids, alkalies, common alcohols and oils). All the experiments were performed on commercial cantilevers used for contact and tapping mode Atomic Force Microscopy (AFM) with stiffness 0.2 N/m and 48 N/m, respectively, in vacuum, air and water. The performance of the setup was evaluated by comparing the measured actuator response to a finite element model. The frequency responses of the two AFM cantilevers measured were compared to analytical equations. A vacuum level of 0.6 mbar was obtained. The setup has a bandwidth of 10-550 kHz in vacuum and air, and a bandwidth of 50-550 kHz in liquid. The dynamic responses of the cantilevers show good agreement with theory in all media., Dynamics of Micro and Nano Systems, Micro and Nano Engineering

Surfactants have the ability to mobilize residual oil trapped in pore spaces of matrix rocks by lowering the oil-water interfacial tension, resulting in a higher oil recovery. However, the loss of surfactants by adsorption onto the rock surface has become a major concern that reduces the efficiency of the surfactant flooding process. In this study, the adsorption behavior of an anionic surfactant to a clay mineral surface was investigated by quartz crystal microbalance with dissipation monitoring upon variations with different cation conditions. Through recording the change of frequency and dissipation of clay-modified sensors, it allows us to do a real-time quantitative analysis of the surfactant adsorption with nanogram sensitivity. The results revealed that the surfactant adsorption increased in a Ca2+-containing solution with increasing pH from 6 to 11, whereas from a Na+-containing solution, more adsorption occurred at acidic conditions. The adsorbed amount went through a maximum (∼200 mM) as a function of the Ca2+ concentration, and the Voigt model suggested that multilayer adsorption of surfactants could be as many as 4-6 monolayers. Using mixed cation (Ca2+ and Na+) solutions, the amount of adsorbed surfactant decreased linearly with decreasing fraction of CaCl2, but Na+ competed for about ∼30% adsorption sites. The importance of the presence of CaCl2 for the surfactant adsorption was stressed in high-salinity and low-salinity solutions in the presence and absence of Ca2+. Furthermore, increasing the temperature from 23 to 65 °C shows first a small increase of surfactant adsorption followed by a reduction of about 20%. The obtained results contribute to a better understanding of surfactant adsorption on clay surfaces and a guide to optimal flooding conditions with reduced surfactant loss., Green Open Access added to TU Delft Institutional Repository'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., OLD ChemE/Organic Materials and Interfaces, Micro and Nano Engineering

Diamond is a highly desirable material for state-of-the-art micro-electromechanical (MEMS) devices, radio-frequency filters and mass sensors, due to its extreme properties and robustness. However, the fabrication/integration of diamond structures into Si-based components remain costly and complex. In this work, a lithography-free, low-cost method is introduced to fabricate diamond-based micro-resonators: a modified home/office desktop inkjet printer is used to locally deposit nanodiamond ink as ∅50–60 µm spots, which are grown into ≈1 µm thick nanocrystalline diamond film disks by chemical vapor deposition, and suspended by reactive ion etching. The frequency response of the fabricated structures is analyzed by laser interferometry, showing resonance frequencies in the range of ≈9–30 MHz, with Q-factors exceeding 104, and (f0 × Q) figure of merit up to ≈2.5 × 1011 Hz in vacuum. Analysis in controlled atmospheres shows a clear dependence of the Q-factors on gas pressure up until 1 atm, with Q ∝ 1/P. When applied as mass sensors, the inkjet-printed diamond resonators yield mass responsivities up to 981 Hz fg−1 after Au deposition, and ultrahigh mass resolution up to 278 ± 48 zg, thus outperforming many similar devices produced by traditional top-down, lithography-based techniques. In summary, this work demonstrates the fabrication of functional high-performance diamond-based micro-sensors by direct inkjet printing., Micro and Nano Engineering, Dynamics of Micro and Nano Systems, QN/Steeneken Lab

Current wafer-scale fabrication methods for graphene-based electronics and sensors involve the transfer of single-layer graphene by a support polymer. This often leaves some polymer residue on the graphene, which can strongly impact its electronic, thermal, and mechanical resonance properties. To assess the cleanliness of graphene fabrication methods, it is thus of considerable interest to quantify the amount of contamination on top of the graphene. Here, we present a methodology for the direct measurement of the mass of the graphene sheet using quartz crystal microbalances (QCMs). By monitoring the QCM resonance frequency during removal of graphene in an oxygen plasma, the total mass of the graphene and contamination is determined with sub-graphene-monolayer accuracy. Since the etch-rate of the contamination is higher than that of graphene, quantitative measurements of the mass of contaminants below, on top, and between graphene layers are obtained. We find that polymer-based dry transfer methods can increase the mass of a graphene sheet by a factor of 10. The presented mass measurement method is conceptually straightforward to interpret and can be used for standardized testing of graphene transfer procedures in order to improve the quality of graphene devices in future applications., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., QN/Steeneken Lab, Electronic Components, Technology and Materials, QN/van der Zant Lab, Dynamics of Micro and Nano Systems, Micro and Nano Engineering

An experimental setup to perform dynamic analysis of a micro- and nano-mechanical system in vacuum, gas, and liquid is presented. The setup mainly consists of a piezoelectric excitation part and the chamber that can be either evacuated for vacuum, or filled with gas or water. The design of the piezoelectric actuator was based on a Langevin transducer. The chamber is made out of materials that can sustain: vacuum, variety of gases and different types of liquids (mild acids, alkalies, common alcohols and oils). All the experiments were performed on commercial cantilevers used for contact and tapping mode Atomic Force Microscopy (AFM) with stiffness 0.2 N/m and 48 N/m, respectively, in vacuum, air and water. The performance of the setup was evaluated by comparing the measured actuator response to a finite element model. The frequency responses of the two AFM cantilevers measured were compared to analytical equations. A vacuum level of 0.6 mbar was obtained. The setup has a bandwidth of 10-550 kHz in vacuum and air, and a bandwidth of 50-550 kHz in liquid. The dynamic responses of the cantilevers show good agreement with theory in all media., Dynamics of Micro and Nano Systems, Micro and Nano Engineering

Desktop inkjet printers are ubiquitous and relatively inexpensive among the variety of available printers. These inkjet printers use an array of micro fluidic pumps, nozzles based on piezoelectric actuation, to dispense individual picoliter volume ink droplets at high speed. In this paper, we show that individual pumps in desktop printers can be accessed to dispense droplets on demand. Access was obtained using the printer’s command language programming. A detailed description of the access procedure is discussed. Droplets were printed on a paper as it rolled underneath the printhead, and with a minor hardware modification, they were also printed on a glass substrate. With this access, individual droplets were deposited, the smallest having an average diameter of 62 μm with a standard deviation of 6.9 μm, with a volume of ∼4 pL. From the intended position, the droplets had a standard deviation of 5.4 μm and 8.4 μm in the vertical and horizontal directions, respectively. The ink droplets were dispensed at a rate of 7.1 kHz. A circularity factor of 0.86 was obtained indicating that the dispensed droplets are of good quality. By replacing the ink in the cartridges with liquids of choice (e.g. cells, proteins, nanoparticles etc.), we believe it provides an opportunity for low-cost, high-speed, high-precision, picoliter volume printing for a variety of applications., Micro and Nano Engineering

Various mechanisms have been proposed to explain the nonphotochemical laser-induced nucleation (NPLIN). Identifying the dominant mechanism requires addressing a large set of experimental parameters with a statistically significant number of samples, forced by the stochastic nature of nucleation. In this study, with aqueous KCl system, we focus on the nucleation probability as a function of laser wavelength, laser intensity, and sample supersaturation, whereas the influence of filtration and the laser-induced radiation pressure on NPLIN activity is also studied. To account for the nucleation stochasticity, we used 80-100 samples. The NPLIN probability showed an increase with increasing laser intensity. The results are different from the previous report, as a supersaturation independent intensity threshold is not observed. No dependence of the NPLIN probability on the laser wavelength (355, 532, and 1064 nm) was observed. Filtration of samples reduced the nucleation probability suggesting a pronounced role of impurities on NPLIN. The magnitude and the propagation velocity of the laser-induced radiation pressure were quantified using a pressure sensor under laser intensities ranging from 0.5 to 80 MW/cm2. No correlation was found between the radiation pressure and NPLIN at our unfocused laser beam intensities ruling out the radiation pressure as a possible cause for nucleation., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Intensified Reaction and Separation Systems, Micro and Nano Engineering

Physical characteristics such as mass and stiffness of biological objects are emerging as new markers for severe diseases. Micromechanical resonators can be used to quantify multiple of these characteristics simultaneously. In this paper, we propose a methodology that utilizes higher flexural modes of vibration to perform simultaneous characterization of the density and elastic modulus of adsorbates. To demonstrate this concept, a polymeric block with a known dimension and anisotropy is written directly on the cantilever surface using a two-photon polymerization technique and characterised by modal analysis. Our method captures the effective bending stress exerted by non-isotropic materials which is masked in the atomic force microscopy indentation technique., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Dynamics of Micro and Nano Systems, QN/Steeneken Lab, Micro and Nano Engineering

The wide-spread adoption of solar thermal absorbers is currently hampered by their low absorption efficiencies and their high capital cost. As a result, a number of initiatives, including direct absorption solar collectors (DASC), are currently underway to improve the absorber efficiencies. In this regard, this study focused on application of hybrid nanofluids containing reduced graphene oxides decorated with silver nanoparticles in volumetric solar absorbers. Their superior solar absorptance and thermal conductivity is based on the plasmonic effect of the nanoparticles and high thermal conductivity of graphene nanosheets, respectively. Several parameters such as mass concentration of graphene nanosheets and Ag decoration contents were studied that could affect the thermal and optical properties of the nanofluids. The results indicated that the prepared nanofluids can be employed for direct absorption solar collectors over a short period of solar irradiation time, even at a low illumination intensity of one sun. A collector efficiency of 77% is achievable at low concentration of 40 ppm owing to the enhanced light absorption of graphene at the excitation wavelength. These findings therefore suggest that this solution can contribute to the final goal of utilizing nanofluids for efficient solar thermal energy harvesting., Energy Technology, Micro and Nano Engineering

Various mechanisms have been proposed to explain the nonphotochemical laser-induced nucleation (NPLIN). Identifying the dominant mechanism requires addressing a large set of experimental parameters with a statistically significant number of samples, forced by the stochastic nature of nucleation. In this study, with aqueous KCl system, we focus on the nucleation probability as a function of laser wavelength, laser intensity, and sample supersaturation, whereas the influence of filtration and the laser-induced radiation pressure on NPLIN activity is also studied. To account for the nucleation stochasticity, we used 80-100 samples. The NPLIN probability showed an increase with increasing laser intensity. The results are different from the previous report, as a supersaturation independent intensity threshold is not observed. No dependence of the NPLIN probability on the laser wavelength (355, 532, and 1064 nm) was observed. Filtration of samples reduced the nucleation probability suggesting a pronounced role of impurities on NPLIN. The magnitude and the propagation velocity of the laser-induced radiation pressure were quantified using a pressure sensor under laser intensities ranging from 0.5 to 80 MW/cm2. No correlation was found between the radiation pressure and NPLIN at our unfocused laser beam intensities ruling out the radiation pressure as a possible cause for nucleation., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Intensified Reaction and Separation Systems, Micro and Nano Engineering

Physical characteristics such as mass and stiffness of biological objects are emerging as new markers for severe diseases. Micromechanical resonators can be used to quantify multiple of these characteristics simultaneously. In this paper, we propose a methodology that utilizes higher flexural modes of vibration to perform simultaneous characterization of the density and elastic modulus of adsorbates. To demonstrate this concept, a polymeric block with a known dimension and anisotropy is written directly on the cantilever surface using a two-photon polymerization technique and characterised by modal analysis. Our method captures the effective bending stress exerted by non-isotropic materials which is masked in the atomic force microscopy indentation technique., Green Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Dynamics of Micro and Nano Systems, QN/Steeneken Lab, Micro and Nano Engineering

The wide-spread adoption of solar thermal absorbers is currently hampered by their low absorption efficiencies and their high capital cost. As a result, a number of initiatives, including direct absorption solar collectors (DASC), are currently underway to improve the absorber efficiencies. In this regard, this study focused on application of hybrid nanofluids containing reduced graphene oxides decorated with silver nanoparticles in volumetric solar absorbers. Their superior solar absorptance and thermal conductivity is based on the plasmonic effect of the nanoparticles and high thermal conductivity of graphene nanosheets, respectively. Several parameters such as mass concentration of graphene nanosheets and Ag decoration contents were studied that could affect the thermal and optical properties of the nanofluids. The results indicated that the prepared nanofluids can be employed for direct absorption solar collectors over a short period of solar irradiation time, even at a low illumination intensity of one sun. A collector efficiency of 77% is achievable at low concentration of 40 ppm owing to the enhanced light absorption of graphene at the excitation wavelength. These findings therefore suggest that this solution can contribute to the final goal of utilizing nanofluids for efficient solar thermal energy harvesting., Energy Technology, Micro and Nano Engineering

A hollow microcantilever is used instead of a conventional microcantilever to dispense and aspirate liquids in the femto-litre (10-15 L) volume range in an atomic force microscope (AFM) setup. The inherent force sensing capability of the cantilever is used to monitor the fluid manipulation in-situ. At this small scale, parameters like: surface energy, evaporation, viscosity and temperature become important for controlled manipulation. In a conventional AFM, these parameters are usually not taken into consideration as feedback parameters. In the present work, we report initial experimental results on the dosing process and an analytical dynamic model of the process. The model is based on the liquid bridge between the cantilever tip and the substrate, which can describe the dosing process with variance accounted for (VAF) larger than 90%. We aim to use this model and implement a control system for precise dispensing and aspiration., Team DeSchutter, Micro and Nano Engineering, Mechatronic Systems Design

Linear and non-linear vibrations of a U-shaped hollow microcantilever beam filled with fluid and interacting with a small particle are investigated. The microfluidic device is assumed to be subjected to internal flowing fluid carrying a buoyant mass. The equations of motion are derived via extended Hamilton's principle and by using Euler-Bernoulli beam theory retaining geometric and inertial non-linearities. A reduced-order model is obtained applying Galerkin's method and solved by using a pseudo arc-length continuation and collocation scheme to perform bifurcation analysis and obtain frequency response curves. Direct time integration of the equations of motion has also been performed by using Adams-Moulton method to obtain time histories and analyze transient cantilever-particle interactions in depth. It is shown that exploiting near resonant non-linear behavior of the microcantilever could potentially yield enhanced sensor metrics. This is found to be due to the transitions that occur as a matter of particle movement near the saddle-node bifurcation points of the coupled system that lead to jumps between coexisting stable attractors., Accepted Author Manuscript, Micro and Nano Engineering

Linear and non-linear vibrations of a U-shaped hollow microcantilever beam filled with fluid and interacting with a small particle are investigated. The microfluidic device is assumed to be subjected to internal flowing fluid carrying a buoyant mass. The equations of motion are derived via extended Hamilton's principle and by using Euler-Bernoulli beam theory retaining geometric and inertial non-linearities. A reduced-order model is obtained applying Galerkin's method and solved by using a pseudo arc-length continuation and collocation scheme to perform bifurcation analysis and obtain frequency response curves. Direct time integration of the equations of motion has also been performed by using Adams-Moulton method to obtain time histories and analyze transient cantilever-particle interactions in depth. It is shown that exploiting near resonant non-linear behavior of the microcantilever could potentially yield enhanced sensor metrics. This is found to be due to the transitions that occur as a matter of particle movement near the saddle-node bifurcation points of the coupled system that lead to jumps between coexisting stable attractors., Accepted Author Manuscript, Micro and Nano Engineering

A hollow microcantilever is used instead of a conventional microcantilever to dispense and aspirate liquids in the femto-litre (10-15 L) volume range in an atomic force microscope (AFM) setup. The inherent force sensing capability of the cantilever is used to monitor the fluid manipulation in-situ. At this small scale, parameters like: surface energy, evaporation, viscosity and temperature become important for controlled manipulation. In a conventional AFM, these parameters are usually not taken into consideration as feedback parameters. In the present work, we report initial experimental results on the dosing process and an analytical dynamic model of the process. The model is based on the liquid bridge between the cantilever tip and the substrate, which can describe the dosing process with variance accounted for (VAF) larger than 90%. We aim to use this model and implement a control system for precise dispensing and aspiration., Team Bart De Schutter, Micro and Nano Engineering, Mechatronic Systems Design

The choice on which type of cantilever to use for Atomic Force Microscopy (AFM) depends on the type of the experiment being done. Typically, the cantilever has to be exchanged when a different stiffness is required and the entire alignment has to be repeated. In the present work, a method to adjust the stiffness in situ of a commercial AFM cantilever is developed. The adjustment is achieved by changing the effective length of the cantilever by electrostatic pull-in. By applying a voltage between the cantilever and an electrode (with an insulating layer at the point of contact), the cantilever snaps to the electrode, reducing the cantilever’s effective length. An analytical model was developed to find the pull-in voltage of the system. Subsequently, a finite element model was developed to study the pull-in behavior. The working principle of this concept is demonstrated with a proof-of-concept experiment. The electrode was positioned close to the cantilever by using a robotic nanomanipulator. To confirm the change in stiffness, the fundamental resonance frequency of the cantilever was measured for varying electrode positions. The results match with the theoretical expectations. The stiffness was adjusted in situ in the range of 0.2 N/m to 27 N/m, covering two orders of magnitude in one single cantilever. This proof-of-concept is the first step towards a micro fabricated prototype, that integrates the electrode positioning system and cantilever that can be used for actual AFM experiments., Micro and Nano Engineering

In situ stiffness adjustment in microelectromechanical systems is used in a variety of applications such as radio-frequency mechanical filters, energy harvesters, atomic force microscopy, vibration detection sensors. In this review we provide designers with an overview of existing stiffness adjustment methods, their working principle, and possible adjustment range. The concepts are categorized according to their physical working principle. It is concluded that the electrostatic adjustment principle is the most applied method, and narrow to wide ranges in stiffness can be achieved. But in order to obtain a wide range in stiffness change, large, complex devices were designed. Mechanical stiffness adjustment is found to be a space-effective way of obtaining wide changes in stiffness, but these methods are often discrete and require large tuning voltages. Stiffness adjustment through stressing effects or change in Young's modulus was used only for narrow ranges. The change in second moment of inertia was used for stiffness adjustment in the intermediate range., Science Education and Communication, Mechatronic Systems Design, Micro and Nano Engineering