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8. A comparative study of 3D-printed scaffolds fabricated from different hydroxyapatite sources for bone tissue engineering applications.

Author

Hoseinpour, Mahdieh, Noori, Alireza, Lotfibakhshaiesh, Nasrin, Nafari, Masoud, Pazhouhnia, Zahra, Baghban Eslaminejad, Mohamadreza, Azami, Mahmoud, and Ghanian, Mohammad Hossein

Subjects
MESENCHYMAL stem cells, YOUNG'S modulus, TISSUE engineering, EXTRACELLULAR matrix, THREE-dimensional printing
Abstract

As a naturally occurring mineral component of bone, hydroxyapatite (HA) is a preferred bone-repair material in clinical medicine. This adaptable biomaterial is developed from synthetic or biological sources. The goal of this study was to investigate the physicochemical and biological properties of scaffolds made from diverse hydroxyapatite sources. We prepared hydroxyapatite powders either from naturally occurring sources, using decellularized bone extracellular matrix (DBECM) and deproteinized bovine bone (DBB), or in the laboratory using the co-precipitation method. Although XRD analysis confirmed the pure phase of HA in all three powders, XRF data found some other trace elements in bone-derived biomaterials, such as magnesium and strontium. Further, FTIR spectra showed DBECM-associated macromolecule peaks. In the second phase of this study, the particles were mixed with sodium alginate to produce bioink for 3D printing via extrusion. SEM photographs of the printed scaffolds showed a grid-like porous structure with large, interconnected pores. Although all of the scaffolds retained their structural integrity after 28 days submerged in the culture medium, DBECM-based scaffolds degraded the most. The test of Young's modulus and compressive strength of scaffolds revealed that all scaffolds are strong enough to be employed in non-weight-bearing applications. The biological characteristics of the scaffolds were then evaluated via cytotoxicity analysis, SEM examination of cell attachment, MTT assay, ALP activity assessment, immunofluorescence staining, and Alizarin red staining. The results indicated that all three scaffolds provided a surface that promoted adhesion and proliferation of rat bone marrow mesenchymal stem cells (rMSCs), as well as stimulated the production of mineralized extracellular matrices. Additionally, our findings showed that scaffolds made from DBECM powders offer the best environment for cell activity. This is most likely due to the presence of trace elements and protein macromolecules in DBECM powders that are not found in DBB and synthetic HA. In conclusion, our comparative study revealed that while all three scaffolds are suitable for bone tissue engineering, DBECM-based scaffolds outperform DBB and synthetic HA. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Tolerance induction by surface immobilization of Jagged-1 for immunoprotection of pancreatic islets

Author

Izadi, Zhila, Hajizadeh-Saffar, Ensiyeh, Hadjati, Jamshid, Habibi-Anbouhi, Mahdi, Ghanian, Mohammad Hossein, Sadeghi-Abandansari, Hamid, Ashtiani, Mohammad Kazemi, Samsonchi, Zakieh, Raoufi, Mohammad, Moazenchi, Maedeh, Izadi, Mahmoud, Nejad, Anava sadat Sadr Hashemi, Namdari, Haideh, Tahamtani, Yaser, Ostad, Seyed Nasser, Akbari-Javar, Hamid, and Baharvand, Hossein

Published
2018
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17. Correction: Firoozi et al. A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction. Biomolecules 2020, 10 , 205.

Author

Firoozi, Saman, Pahlavan, Sara, Ghanian, Mohammad-Hossein, Rabbani, Shahram, Tavakol, Shima, Barekat, Maryam, Yakhkeshi, Saeed, Mahmoudi, Elena, Soleymani, Mansoureh, and Baharvand, Hossein

Subjects
STAINS & staining (Microscopy), MESENCHYMAL stem cells, AMINO acid sequence, MYOCARDIAL infarction, PEPTIDES
Abstract

This correction notice addresses errors in a scientific paper titled "A Cell-Free SDKP-Conjugated Self-Assembling Peptide Hydrogel Sufficient for Improvement of Myocardial Infarction." The authors discovered that some representative images in Figures 4 and 5 overlapped with images from another published paper. This occurred because the experiments were performed simultaneously. The authors apologize for any inconvenience caused and state that the scientific conclusions were unaffected. The corrected version of the paper will be updated on the article webpage. Additionally, a correction was made to the peptide sequence in the Materials and Methods section due to intellectual property issues. The paper includes graphs showing the effects of the hydrogel on scar size, angiogenesis, and inflammation. [Extracted from the article]

Published
2024
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23. Tissue-Specific Microparticles Improve Organoid Microenvironment for Efficient Maturation of Pluripotent Stem-Cell-Derived Hepatocytes.

Author

UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique, Zahmatkesh, Ensieh, Ghanian, Mohammad Hossein, Zarkesh, Ibrahim, Farzaneh, Zahra, Halvaei, Majid, Heydari, Zahra, Moeinvaziri, Farideh, Othman, Amnah, Ruoß, Marc, Piryaei, Abbas, Gramignoli, Roberto, Yakhkeshi, Saeed, Nüssler, Andreas, Najimi, Mustapha, Baharvand, Hossein, Vosough, Massoud, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - (SLuc) Service de gastro-entérologie et hépatologie pédiatrique, Zahmatkesh, Ensieh, Ghanian, Mohammad Hossein, Zarkesh, Ibrahim, Farzaneh, Zahra, Halvaei, Majid, Heydari, Zahra, Moeinvaziri, Farideh, Othman, Amnah, Ruoß, Marc, Piryaei, Abbas, Gramignoli, Roberto, Yakhkeshi, Saeed, Nüssler, Andreas, Najimi, Mustapha, Baharvand, Hossein, and Vosough, Massoud

Abstract

Liver organoids (LOs) are receiving considerable attention for their potential use in drug screening, disease modeling, and transplantable constructs. Hepatocytes, as the key component of LOs, are isolated from the liver or differentiated from pluripotent stem cells (PSCs). PSC-derived hepatocytes are preferable because of their availability and scalability. However, efficient maturation of the PSC-derived hepatocytes towards functional units in LOs remains a challenging subject. The incorporation of cell-sized microparticles (MPs) derived from liver extracellular matrix (ECM), could provide an enriched tissue-specific microenvironment for further maturation of hepatocytes inside the LOs. In the present study, the MPs were fabricated by chemical cross-linking of a water-in-oil dispersion of digested decellularized sheep liver. These MPs were mixed with human PSC-derived hepatic endoderm, human umbilical vein endothelial cells, and mesenchymal stromal cells to produce homogenous bioengineered LOs (BLOs). This approach led to the improvement of hepatocyte-like cells in terms of gene expression and function, CYP activities, albumin secretion, and metabolism of xenobiotics. The intraperitoneal transplantation of BLOs in an acute liver injury mouse model led to an enhancement in survival rate. Furthermore, efficient hepatic maturation was demonstrated after ex ovo transplantation. In conclusion, the incorporation of cell-sized tissue-specific MPs in BLOs improved the maturation of human PSC-derived hepatocyte-like cells compared to LOs. This approach provides a versatile strategy to produce functional organoids from different tissues and offers a novel tool for biomedical applications.

Published
2021

24. Tissue-Specific Microparticles Improve Organoid Microenvironment for Efficient Maturation of Pluripotent Stem-Cell-Derived Hepatocytes

Author

Zahmatkesh, Ensieh, primary, Ghanian, Mohammad Hossein, additional, Zarkesh, Ibrahim, additional, Farzaneh, Zahra, additional, Halvaei, Majid, additional, Heydari, Zahra, additional, Moeinvaziri, Farideh, additional, Othman, Amnah, additional, Ruoß, Marc, additional, Piryaei, Abbas, additional, Gramignoli, Roberto, additional, Yakhkeshi, Saeed, additional, Nüssler, Andreas, additional, Najimi, Mustapha, additional, Baharvand, Hossein, additional, and Vosough, Massoud, additional

Published
2021
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25. Additional file 1 of Dual functional construct containing kartogenin releasing microtissues and curcumin for cartilage regeneration

Author

Negin Asgari, Bagheri, Fatemeh, Mohamadreza Baghaban Eslaminejad, Ghanian, Mohammad Hossein, Forogh Azam Sayahpour, and Ghafari, Amir Mohammad

Abstract

Additional file 1: Table S1. Primer sequences used for real time RT-PCR. Figure S1. Light microscopic images of cell aggregates on days 1 and 21. Figure S2.COL1A1 and COL2A1 expression in MSCs aggregate cultures. The expression of COL1A1 and COL2A1 in [MSC/KGN-MP] Agg group and [MSC] Agg in the chondrogenic medium compared to the control (cell aggregate). The ratio of COL2/COL1 expression showed the effects of KGN in hyaline cartilage formation. Figure S3. Cell viability of MSCs at various concentrations of curcumin (0, 10, 20, 30 and 40 μM) was examined by MTT assay for 48 h. Data are shown as mean ± SD, **p

Published
2020
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27. Scalable and cost-effective generation of osteogenic micro-tissues through the incorporation of inorganic microparticles within mesenchymal stem cell spheroids

Author

Zarkesh, Ibrahim, primary, Halvaei, Majid, additional, Ghanian, Mohammad Hossein, additional, Bagheri, Fatemeh, additional, Sayahpour, Forough Azam, additional, Azami, Mahmoud, additional, Mohammadi, Javad, additional, Baharvand, Hossein, additional, and Baghaban Eslaminejad, Mohamadreza, additional

Published
2019
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32. Polycaprolactone-templated reduced-graphene oxide liquid crystal nanofibers towards biomedical applications

Author

Jalili-Firoozinezhad, Sasan, Hasan Mohamadzadeh Moghadam, Mohamad, Ghanian, Mohammad Hossein, Ashtiani, Mohammad Kazemi, Alimadadi, Hossein, Baharvand, Hossein, Martin, Ivan, Scherberich, Arnaud, Jalili-Firoozinezhad, Sasan, Hasan Mohamadzadeh Moghadam, Mohamad, Ghanian, Mohammad Hossein, Ashtiani, Mohammad Kazemi, Alimadadi, Hossein, Baharvand, Hossein, Martin, Ivan, and Scherberich, Arnaud

Abstract

Here, we report a facile method to generate electrically conductive nanofibers by coating and subsequently chemically reducing graphene oxide (GO) liquid crystals on a polycaprolactone (PCL) mat. Ultra large GO sheets obtained are in favor of charge carrier mobility and oriented morphology of the GO coating. We showed that coating the reduced GO (rGO) not only retains the three-dimensional topography, fiber orientation and size of the template PCL, but also makes it electroconductive. Our preliminary in vitro assessments using mesenchymal stem cells revealed no induced cytotoxicity yet increased cellular metabolism on PCL-templated rGO fibers.

Published
2017

34. Possibilities in Germ Cell Research: An Engineering Insight

Author

Esfandiari, Fereshteh, Mashinchian, Omid, Ashtiani, Mohammad Kazemi, Ghanian, Mohammad Hossein, Hayashi, Katsuhiko, Saei, Amir Ata, Mahmoudi, Morteza, Baharvand, Hossein, Esfandiari, Fereshteh, Mashinchian, Omid, Ashtiani, Mohammad Kazemi, Ghanian, Mohammad Hossein, Hayashi, Katsuhiko, Saei, Amir Ata, Mahmoudi, Morteza, and Baharvand, Hossein

Abstract

Germ cells (GCs) are responsible for fertility and disruptions in their development or function cause infertility. However, current knowledge about the diverse mechanisms involved in GC development and function is still in its infancy. This is mainly because there are low numbers of GCs, especially during embryonic development. A deeper understanding of GCs would enhance our ability to produce them from stem cells. In addition, such information would enable the production of healthy gametes for infertile couples. In this regard, pluripotent stem cells (PSCs) demonstrated a promising ability to produce GCs in vitro. In this review, we highlight recent advances in the field of tissue engineering that suggest novel strategies to enhance GC research.

Published
2016
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39. Possibilities in Germ Cell Research: An Engineering Insight

Author

Esfandiari, Fereshteh, Mashinchian, Omid, Ashtiani, Mohammad Kazemi, Ghanian, Mohammad Hossein, Hayashi, Katsuhiko, Saei, Amir Ata, Mahmoudi, Morteza, and Baharvand, Hossein

Subjects
endocrine system, hormones, hormone substitutes, and hormone antagonists
Abstract

Germ cells (GCs) are responsible for fertility and disruptions in their development or function cause infertility. However, current knowledge about the diverse mechanisms involved in GC development and function is still in its infancy. This is mainly because there are low numbers of GCs, especially during embryonic development. A deeper understanding of GCs would enhance our ability to produce them from stem cells. In addition, such information would enable the production of healthy gametes for infertile couples. In this regard, pluripotent stem cells (PSCs) demonstrated a promising ability to produce GCs in vitro. In this review, we highlight recent advances in the field of tissue engineering that suggest novel strategies to enhance GC research.

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