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6 results on '"Ghandour, Salah"'

1. Endothelin receptor blockers reduce I/R-induced intestinal mucosal injury: role of blood flow

Author

Oktar, Berna K., Gulpinar, M. Ali, Bozkurt, Ayhan, Ghandour, Salah, Cetinel, Sule, Moini, Hadi, Yegen, Berrak C., Bilsel, Serpil, Granger, D. Neil, and Kurtel, Hizir

Subjects
Endothelin -- Physiological aspects, Ischemia -- Physiological aspects, Mucous membrane -- Injuries, Biological sciences
Abstract

The aim of the present study was to assess the role of endothelin (ET) in ischemia-reperfusion (I/R)-induced mucosal injury. Mucosal permeability ([sup.51]Cr-EDTA clearance) and tissue myeloperoxidase (MPO) activity were significantly increased after 30 rain of ischemia followed by 30 min of reperfusion. The I/R-induced increases in mucosal permeability and polymorphonuclear leukocyte (PMN) infiltration were significantly attenuated by pretreatments with E[T.sub.A] (BQ-485) and/or E[T.sub.B] (BQ-788) receptor antagonists. Monoclonal antibody (MAb) directed against intercellular adhesion molecule-1 (ICAM-1; MAb 1A29) and superoxide dismutase (SOD) pretreatments significantly attenuated the increased mucosal permeability and PMN infiltration in a similar manner as with ET receptor antagonists. Superior mesenteric artery blood flow was significantly reduced during the reperfusion period. Both ET receptor antagonists caused a significant rise in blood flow compared with an untreated I/R group. In conclusion, our data suggest that E[T.sub.A] and/or E[T.sub.B] receptors, ICAM-1, and superoxide play an important role in I/R-induced mucosal dysfunction and PMN infiltration. Furthermore, ET is involved in the pathogenesis of postreperfusion-induced damage and beneficial effects of ET receptor antagonism are related to an improvement of disturbed blood flow during the reperfusion period. mucosal permeability; mucosal injury; BQ-485; BQ-788; superoxide dismutase

Published
2002

4. Ameliyat sonrası karın içi yapışıklık oluşumunda bağırsak kan akımındaki değişiklikler ve Endotelin-1 bloğunun rolü

Author

Günal, Ömer, Ghandour, Salah, Deniz, Mustafa, Aslaner, Arif, Düzce Üniversitesi Tıp Fakültesi, Günal, Ömer, and Aslaner, Arif

Subjects
Sıçan, Endotelin, Adhesions, Endothelins, Reseptör, Receptors, Wistar, Bosentan, Endotelin/Antagonist-inhibitör, Yapışıklık, Wistar Cinsi, Rats, Endothelin/Antagonists & Inhibitors
Abstract

AMAÇ: Ameliyat sonrası periton içi yapışıklık oluşumunda bağırsak kan akımı değişikliklerini incelemek ve Endotelin-1 (ET-1) reseptör blokajının rolünü araştırmaktır. GEREÇ-YÖNTEM: Çalışmada 250-300 gr ağırlığında 28 adet Wistar-Albino erişkin sıçan eşit olarak dört gruba ayrıldı. Kontrol grubuna (grup 1; n=7) ameliyat uygulanmadı. Sham grubuna (grup 2; n=7) sadece laparotomi yapıldı. Yapışıklık grubuna (grup 3; n=7) sağ karın duvarından 1x1 cm’lik peritoneal yama eksizyonu ve çekal abrazyon, “yapışıklık model ameliyatı” olarak uygulandı. Tedavi grubuna (grup 4; n=7); bir non-selektif ET-1 reseptör blokeri olan bosentan (30 mg/kg, IP) periton içi yoldan dört gün süreyle günde bir kez verildi. Ameliyat sonrası yedinci gün, superior mezenterik arterin beslediği bağırsak kan akımı ölçüldü. Yapışıklık şiddeti ve yaygınlığı ile yapışıklıktaki miyeloperoksidaz (MPO) aktivitesi ölçüldü. BULGULAR: Ortalama bağırsak kan akımı grup 1 (65.5±1.2 ml/100 gr) ile kıyaslandığında yapışıklık grubunda (81.9±5.6 ml/100 gr) belirgin olarak artmıştı. Grup 1 ve grup 2 ile karşılaştırıldığında bosentanın (44.3±6.9 ml/100 gr) bağırsak kan akımında belirgin azalmaya neden olduğu görüldü. Sham grubunun (62.2±1 ml/100 gr) kan akım seviyeleri kontrol grubundakilerle (65.5±1.2 ml/100 gr) benzerlik gösterdi. Yapışıklık skorları, yapışıklık ve bosentan gruplarında benzer bulundu. Sham grubunda neredeyse hiç yapışıklık görülmedi. Bosentan grubunda yapışıklık dokusu MPO aktivitesi belirgin olarak artmıştı. SONUÇ: Non-selektif ET-1 reseptör blokajının periton içi yapışıklık oluşumunun önlenmesinde bir etkisi yoktur ancak, bağırsak kan akımında azalmaya neden olur. Yapışıklık oluşumu bağırsak kan akımını arttırır. Yapışıklık oluşumu, bosentan tedavisine rağmen artmış polimorfonükleer (PMN) infiltrasyonu ile birliktedir. BACKGROUND: The current study was planned to investigate intestinal blood flow alterations and the role of ET-1 receptor blockade in the formation of postoperative intraperitoneal adhesion formation. METHODS: Twenty-eight adult Wistar Albino rats weighing between 250 g and 300 g were divided into four groups. Control group (group 1; n=7) did not undergo any operation. Sham group (group 2; n=7) had only laparotomy. In the adhesion group (group 3; n=7), peritoneal patch (1x1 cm) excision from the right abdominal wall and cecal abrasion were done as "adhesion model operation". One week following this, treatment group (group 4; n=7) received a non-selective ET-1 receptor blocking agent bosentan (30 mg/kg, IP) intra-abdominally, once a day for four days. Intestinal blood flow through the superior mesenteric artery was measured, on postoperative seventh day. Adhesion severity and extension as well as myeloperoxidase activity in the adhesion were calculated. RESULTS: Mean intestinal blood flow significantly increased in adhesion group (81.9±5.6 ml/100 g) when compared to group 1 (65.5±1.2 ml/100 g). Bosentan caused a significant decrease (44.3±6.9 ml/100 g) in intestinal blood flow when compared to group 1 and group 2. Sham group (62.2±1 ml/100 g) had similar blood flow level with the control group (65.5±1.2 ml/100g). Adhesion scores were similar in adhesion and Bosentan groups. Sham group had almost no adhesions. Myeloperoxidase activity in adhesion tissue was significantly higher in bosentan group. CONCLUSION: Non-selective ET-1 receptor blockade has no effect on prevention of the formation of intra-abdominal adhesion, but causes a decrease in intestinal blood flow. Adhesion formation increases intestinal blood flow. Adhesion formation is accompanied by increased polymorphonuclear infiltration despite bosentan treatment.

Published
2006

6. [Intestinal blood flow alterations in postoperative intraabdominal adhesion formation and the role of Endothelin-1 blockade].

Author

Günal O, Ghandour S, Deniz M, and Aslaner A

Subjects
Animals, Antihypertensive Agents pharmacology, Blood Flow Velocity, Bosentan, Disease Models, Animal, Endothelin-1 antagonists & inhibitors, Intestines drug effects, Peritoneal Diseases prevention & control, Postoperative Complications prevention & control, Pulsatile Flow, Rats, Rats, Wistar, Sulfonamides pharmacology, Tissue Adhesions physiopathology, Tissue Adhesions prevention & control, Abdomen surgery, Intestines blood supply, Mesenteric Arteries physiology, Peritoneal Diseases physiopathology, Postoperative Complications physiopathology
Abstract

Background: The current study was planned to investigate intestinal blood flow alterations and the role of ET-1 receptor blockade in the formation of postoperative intraperitoneal adhesion formation., Methods: Twenty-eight adult Wistar Albino rats weighing between 250 g and 300 g were divided into four groups. Control group (group 1; n=7) did not undergo any operation. Sham group (group 2; n=7) had only laparotomy. In the adhesion group (group 3; n=7), peritoneal patch (1x1 cm) excision from the right abdominal wall and cecal abrasion were done as "adhesion model operation". One week following this, treatment group (group 4; n=7) received a non-selective ET-1 receptor blocking agent bosentan (30 mg/kg, IP) intra-abdominally, once a day for four days. Intestinal blood flow through the superior mesenteric artery was measured, on postoperative seventh day. Adhesion severity and extension as well as myeloperoxidase activity in the adhesion were calculated., Results: Mean intestinal blood flow significantly increased in adhesion group (81.9+/-5.6 ml/100 g) when compared to group 1 (65.5+/-1.2 ml/100 g). Bosentan caused a significant decrease (44.3+/-6.9 ml/100 g) in intestinal blood flow when compared to group 1 and group 2. Sham group (62.2+/-1 ml/100 g) had similar blood flow level with the control group (65.5+/-1.2 ml/100 g). Adhesion scores were similar in adhesion and Bosentan groups. Sham group had almost no adhesions. Myeloperoxidase activity in adhesion tissue was significantly higher in bosentan group., Conclusion: Non-selective ET-1 receptor blockade has no effect on prevention of the formation of intra-abdominal adhesion, but causes a decrease in intestinal blood flow. Adhesion formation increases intestinal blood flow. Adhesion formation is accompanied by increased polymorphonuclear infiltration despite bosentan treatment.

Published
2006

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