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20 results on '"Ghali, Maged P."'

1. HIV-1 is rarely detected in blood and colon myeloid cells during viral-suppressive antiretroviral therapy

Author

Cattin, Amélie, Wiche Salinas, Tomas Raul, Gosselin, Annie, Planas, Delphine, Shacklett, Barbara, Cohen, Eric A, Ghali, Maged P, Routy, Jean-Pierre, and Ancuta, Petronela

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, HIV/AIDS, Infectious Diseases, Sexually Transmitted Infections, Clinical Research, 2.1 Biological and endogenous factors, Infection, Adult, Aged, Anti-Retroviral Agents, Blood Cells, Colon, Sigmoid, Female, HIV Infections, HIV-1, Humans, Male, Middle Aged, Myeloid Cells, Sustained Virologic Response, Viral Load, Young Adult, antiretroviral therapy, CD16(+) monocytes, CD1(+) dendritic cell, HIV reservoirs, myeloid cells, Biological Sciences, Medical and Health Sciences, Psychology and Cognitive Sciences, Virology, Biomedical and clinical sciences, Health sciences
Abstract

ObjectiveThe aim of this study was to explore the contribution of blood and colon myeloid cells to HIV persistence during antiretroviral therapy (ART).DesignLeukapheresis was collected from HIV-infected individuals with undetectable plasma viral load during ART (HIV + ART; n = 15) and viremics untreated (HIV+; n = 6). Rectal sigmoid biopsies were collected from n = 8 HIV+ART.MethodsMyeloid cells (total monocytes (Mo), CD16/CD16 Mo, CD1c dendritic cells) and CD4 T cells were isolated by magnetic-activated cell sorting (MACS) and/or fluorescence-activated cell sorting (FACS) from peripheral blood. Matched myeloid and CCR6CD4 T cells were isolated from blood and rectal biopsies by FACS. Levels of early (RU5 primers), late (Gag primers) and/or integrated HIV-DNA (Alu/HIV primers) were quantified by nested real-time PCR. Replication-competent HIV was amplified by co-culturing cells from HIV-positive individuals with CD3/CD28-activated CD4 T cells from uninfected donors.ResultsEarly/late but not integrated HIV reverse transcripts were detected in blood myeloid subsets of four out of 10 HIV+ART; in contrast, integrated HIV-DNA was exclusively detected in CD4 T cells. In rectal biopsies, late HIV reverse transcripts were detected in myeloid cells and CCR6CD4 T cells from one out of eight and seven out of eight HIV+ART individuals, respectively. Replication-competent HIV was outgrown from CD4 T cells but not from myeloid of untreated/ART-treated HIV-positive individuals.ConclusionIn contrast to CD4 T cells, blood and colon myeloid cells carry detectable HIV only in a small fraction of HIV+ART individuals. This is consistent with the documented resistance of Mo to HIV infection and the rapid turnover of Mo-derived macrophages in the colon. Future assessment of multiple lymphoid and nonlymphoid tissues is required to include/exclude myeloid cells as relevant HIV reservoirs during ART.

Published
2019

2. HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy

Author

Gosselin, Annie, Salinas, Tomas Raul Wiche, Planas, Delphine, Wacleche, Vanessa S, Zhang, Yuwei, Fromentin, Rémi, Chomont, Nicolas, Cohen, Éric A, Shacklett, Barbara, Mehraj, Vikram, Ghali, Maged P, Routy, Jean-Pierre, and Ancuta, Petronela

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Sexually Transmitted Infections, HIV/AIDS, Infectious Diseases, Infection, Adult, Aged, Anti-Retroviral Agents, Blood, CD4-Positive T-Lymphocytes, Colon, DNA, Viral, Female, Flow Cytometry, HIV Infections, Humans, Male, Middle Aged, Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction, Receptors, CCR6, T-Lymphocyte Subsets, Virus Activation, Young Adult, antiretroviral therapy, CCR6, central memory CD4(+) T cells, HIV reservoirs, retinoic acid, Th17, viral outgrowth assay, Biological Sciences, Medical and Health Sciences, Psychology and Cognitive Sciences, Virology, Biomedical and clinical sciences, Health sciences
Abstract

ObjectivesThe objective of this article is to investigate the contribution of colon and blood CD4 T-cell subsets expressing the chemokine receptor CCR6 to HIV persistence during antiretroviral therapy.DesignMatched sigmoid biopsies and blood samples (n = 13) as well as leukapheresis (n = 20) were collected from chronically HIV-infected individuals receiving antiretroviral therapy. Subsets of CD4 T cells with distinct differentiation/polarization profiles were identified using surface markers as follows: memory (TM, CD45RA), central memory (TCM; CD45RACCR7), effector (TEM/TM; CD45RACCR7), Th17 (CCR6CCR4), Th1Th17 (CCR6CXCR3), Th1 (CCR6CXCR3), and Th2 (CCR6CCR4).MethodsWe used polychromatic flow cytometry for cell sorting, nested real-time PCR for HIV DNA quantification, ELISA and flow cytometry for HIV p24 quantification. HIV reactivation was induced by TCR triggering in the presence/absence of all-trans retinoic acid.ResultsCompared with blood, the frequency of CCR6 TM was higher in the colon. In both colon and blood compartments, CCR6 TM were significantly enriched in HIV DNA when compared with their CCR6 counterparts (n = 13). In blood, integrated HIV DNA levels were significantly enriched in CCR6 versus CCR6 TCM of four of five individuals and CCR6 versus CCR6 TEM of three of five individuals. Among blood TCM, Th17 and Th1Th17 contributed the most to the pool of cells harboring integrated HIV DNA despite their reduced frequency compared with Th2, which were infected the least. HIV reactivation was induced by TCR triggering and/or retinoic acid exposure at higher levels in CCR6 versus CCR6 TM, TCM, and TEM.ConclusionCCR6 is a marker for colon and blood CD4 T cells enriched for replication-competent HIV DNA. Novel eradication strategies should target HIV persistence in CCR6CD4 T cells from various anatomic sites.

Published
2017

3. Distribution and inflammatory potential of hepatitis C virus genotypes in the United States, 2011–2020.

Author

Gnanapandithan, Karthik, Stemboroski, Lauren, Johnston, Abbey, and Ghali, Maged P.

Subjects
HEALTH & Nutrition Examination Survey, HEPATIC fibrosis, HEPATITIS C virus, ASPARTATE aminotransferase, GENETIC variation, HEPATITIS C
Abstract

The study analyzed data from 266 HCV-infected adults in the United States from 2011 to 2020, revealing that genotype 1a is the most prevalent at 60.2%. Genotype 3 was associated with higher transaminase levels, indicating a potentially more aggressive disease phenotype. The findings underscore the importance of ongoing surveillance for HCV genotypes and considering genotype-specific treatment and monitoring strategies to optimize management and improve outcomes for patients. [Extracted from the article]

Published
2024
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11. S1494 Gastroenterology Fellowship Virtual Interviews: Applicant and Faculty Perceptions on Virtual Interview Advantages and Barriers

Author

Khoudari, George, primary, Eltelbany, Ahmed, additional, Salem, George, additional, Hakim, Seifeldin, additional, Narayanan, Shreya, additional, Abegunde, Ayokunle T., additional, Mahmood, Sultan, additional, Hong, Simon, additional, Khan, Freeha, additional, Bhurwal, Abhishek, additional, Meriwether, Matthew, additional, Haddad, Nael, additional, Alishahi, Yasmin, additional, Ghali, Maged P., additional, Tierney, William, additional, Sifuentes, Humberto, additional, DuPont, Andrew W., additional, Vega, Kenneth J., additional, Regueiro, Miguel, additional, and Mattar, Mark C., additional

Published
2022
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14. Esophageal Perforation after Using a Single-Use Disposable Duodenoscope

Author

Cheema, Bakht S., Ghali, Maged, Schey, Ron, Awad, Ziad, and Ribeiro, Bruno

Abstract

The Food and Drug Administration (FDA) has recently released a safety communication recommending transition to duodenoscopes with innovative designs that facilitate or eliminate the need for reprocessing. Thus, there has been a significant amount of development into disposable duodenoscope components and single-use duodenoscopes, with variable tactile feedback. We describe a case of esophageal perforation after using a single-use disposable duodenoscope (EXALT Model D; Boston Scientific Corporation, Marlborough, MA, USA). To our knowledge, this is the first reported case of an esophageal perforation since FDA approval of this device in December 2019. ERCP was performed with the EXALT Model D single-use duodenoscope (Boston Scientific Corporation) by an experienced gastroenterologist. During the procedure, gentle force applied through the gastroesophageal junction caused a liner perforation in the distal esophagus. An esophageal stent was placed with satisfactory wound healing and no fistula formation. There have been a few reports in the last 2 years showing promising results using this device; however, the differences in the tactile feedback, navigation, and pushability of the device may make it prone to unintended consequences.

Published
2021
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15. Sofosbuvir-Based Antiviral Therapy Is Highly Effective In Recurrent Hepatitis C in Liver Transplant Recipients

Author

Faisal, Nabiha, primary, Bilodeau, Marc, additional, Aljudaibi, Bandar, additional, Hirsch, Geri, additional, Yoshida, Eric M., additional, Hussaini, Trana, additional, Ghali, Maged P., additional, Congly, Stephen E., additional, Ma, Mang M., additional, Leonard, Jennifer, additional, Cooper, Curtis, additional, Peltekian, Kevork, additional, Renner, Eberhard L., additional, and Lilly, Leslie B., additional

Published
2016
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17. Obeticholic acid for the treatment of non-alcoholic steatohepatitis: interim analysis from a multicentre, randomised, placebo-controlled phase 3 trial

Author

Younossi, Zobair M, Ratziu, Vlad, Loomba, Rohit, Rinella, Mary, Anstee, Quentin M, Goodman, Zachary, Bedossa, Pierre, Geier, Andreas, Beckebaum, Susanne, Newsome, Philip N, Sheridan, David, Sheikh, Muhammad Y, Trotter, James, Knapple, Whitfield, Lawitz, Eric, Abdelmalek, Manal F, Kowdley, Kris V, Montano-Loza, Aldo J, Boursier, Jerome, Mathurin, Philippe, Bugianesi, Elisabetta, Mazzella, Giuseppe, Olveira, Antonio, Cortez-Pinto, Helena, Graupera, Isabel, Orr, David, Gluud, Lise Lotte, Dufour, Jean-Francois, Shapiro, David, Campagna, Jason, Zaru, Luna, MacConell, Leigh, Shringarpure, Reshma, Harrison, Stephen, Sanyal, Arun J, Abdelmalek, Manal, Abrams, Gary, Aguilar, Humberto, Ahmed, Aijaz, Aigner, Elmar, Aithal, Guruprasad, Ala, Aftab, Alazawi, William, Albillos, Agustin, Allison, Michael, Al-Shamma, Sfa, Andrade, Raul, Andreone, Pietro, Angelico, Mario, Ankoma-Sey, Victor, Anstee, Quentin, Anty, Rodolphe, Araya, Victor, Arenas Ruiz, Juan Ignacio, Arkkila, Perttu, Arora, Marty, Asselah, Tarik, Au, Jennifer, Ayonrinde, Oyekoya, Bailey, Robert James, Balakrishnan, Maya, Bambha, Kiran, Bansal, Meena, Barritt, Sidney, Bate, John, Beato, Jorge, Beckebaum, Susanne, Behari, Jaideep, Bellot, Pablo, Ben Ari, Ziv, Bennett, Michael, Berenguer, Marina, Beretta-Piccoli, Benedetta Terziroli, Berg, Thomas, Bonacini, Maurizio, Bonet, Lucia, Borg, Brian, Bourliere, Marc, Boursier, Jerome, Bowman, William, Bradley, David, Brankovic, Marija, Braun, Marius, Bronowicki, Jean-Pierre, Bruno, Savino, Bugianesi, Elisabetta, Cai, Cindy, Calderon, Amy, Calleja Panero, José Luis, Carey, Elizabeth, Carmiel, Michal, Carrión, Jose Antonio, Cave, Matthew, Chagas, Cristina, Chami, Tawfik, Chang, Alan, Coates, Allan, Cobbold, Jeremy, Costentin, Charlote, Corey, Kathleen, Corless, Lynsey, Cortez-Pinto, Helena, Crespo, Javier, Cruz Pereira, Oscar, de Ledinghen, Victor, deLemos, Andrew, Diago, Moises, Dong, Mamie, Dufour, Jean-François, Dugalic, Predrag, Dunn, Winston, Elkhashab, Magby, Epstein, Michael, Escudero-Garcia, Maria Desamparados, Etzion, Ohad, Evans, Larry, Falcone, Robert, Fernandez, Conrado, Ferreira, Jose, Fink, Scott, Finnegan, Kevin, Firpi-Morell, Roberto, Floreani, Annarosa, Fontanges, Thierry, Ford, Ryan, Forrest, Ewan, Fowell, Andrew, Fracanzani, Anna Ludovica, Francque, Sven, Freilich, Bradley, Frias, Juan, Fuchs, Michael, Fuentes, Javier, Galambos, Michael, Gallegos, Juan, Geerts, Anja, Geier, Andreas, George, Jacob, Ghali, Maged, Ghalib, Reem, Gholam, Pierre, Gines, Pere, Gitlin, Norman, Gluud, Lise Lotte, Goeser, Tobias, Goff, John, Gordon, Stuart, Gordon, Frederic, Goria, Odile, Greer, Shaun, Grigorian, Alla, Gronbaek, Henning, Guillaume, Maeva, Gunaratnam, Naresh, Halegoua-De Marzio, Dina, Hameed, Bilal, Hametner, Stephanie, Hamilton, James, Harrison, Stephen, Hartleb, Marek, Hassanein, Tarek, Häussinger, Dieter, Hellstern, Paul, Herring, Robert, Heurich, Eva, Hezode, Christophe, Hinrichsen, Holger, Holland Fischer, Peter, Horsmans, Yves, Huang, Jonathan, Hussaini, Hyder, Jakiche, Antoine, Jeffers, Lennox, Jones, Blake, Jorge, Rosa, Jorquera, Francisco, Joshi, Shoba, Kahraman, Alisan, Kaita, Kelly, Karyotakis, Nicholas, Kayali, Zeid, Kechagias, Stergios, Kepczyk, Thomas, Khalili, Mandana, Khallafi, Hicham, Kluwe, Johannes, Knapple, Whitfield, Kohli, Anita, Korenblat, Kevin, Kowdley, Kris, Krag, Aleksander, Krause, Richard, Kremer, Andreas, Krok, Karen, Krstic, Miodrag, Kugelmas, Marcelo, Kumar, Sonal, Kuwada, Scott, Labarriere, Damien, Lai, Michelle, Laleman, Wim, Lampertico, Pietro, Lawitz, Eric, Lee, Alice, Leroy, Vincent, Lidofsky, Steven, Lim, Tina Huey, Lim, Joseph, Lipkis, Donald, Little, Ester, Lonardo, Amadeo, Long, Michelle, Loomba, Rohit, Luketic, Velimir Anthony Christopher, Lurie, Yoav, Macedo, Guilherme, Magalhaes, Joana, Makara, Mihály, Maliakkal, Benedict, Manns, Michael, Manousou, Pinelopi, Mantry, Parvez, Marchesini, Giulio, Marinho, Carla, Marotta, Paul, Marschall, Hanns-Ulrich, Martinez, Linda, Mathurin, Philippe, Mayo, Marlyn, Mazzella, Giuseppe, McCullen, Mark, McLaughlin, William, Merle, Uta, Merriman, Raphael, Modi, Apurva, Molina, Esther, Montano-Loza, Aldo, Monteverde, Carlos, Morales Cardona, Amilcar, Moreea, Sulleman, Moreno, Christophe, Morisco, Filomena, Mubarak, Abdullah, Muellhaupt, Beat, Mukherjee, Sandeep, Müller, Tobias, Nagorni, Aleksandar, Naik, Jahnavi, Neff, Guy, Nevah, Moises, Newsome, Philip, Nguyen-Khac, Eric, Noureddin, Mazen, Oben, Jude, Olveira, Antonio, Orlent, Hans, Orr, David, Orr, James, Ortiz-Lasanta, Grisell, Ozenne, Violaine, Pandya, Prashant, Paredes, Angelo, Park, James, Patel, Joykumar, Patel, Keyur, Paul, Sonali, Patton, Heather, Peck-Radosavljevic, Markus, Petta, Salvatore, Pianko, Stephen, Piekarska, Anna, Pimstone, Neville, Pisegna, Joseph, Pockros, Paul, Pol, Stanislas, Porayko, Michael, Poulos, John, Pound, David, Pouzar, Joe, Presa Ramos, Jose, Pyrsopoulos, Nikolaos, Rafiq, Nila, Muller, Kate, Ramji, Alnoor, Ratziu, Vlad, Ravinuthala, Ravi, Reddy, Chakradhar, Reddy K G, Gautham, Reddy K R, K. Rajender, Regenstein, Frederic, Reindollar, Robert, Reynolds, Justin, Riera, Andres, Rinella, Mary, Rivera Acosta, Jose, Robaeys, Geert, Roberts, Stuart, Rodriguez-Perez, Federico, Romero, Sandor, Romero-Gomez, Manuel, Rubin, Raymond, Rumi, Mariagrazia, Rushbrook, Simon, Rust, Christian, Ryan, Michael, Safadi, Rifaat, Said, Adnan, Salminen, Kimmo, Samuel, Didier, Santoro, John, Sanyal, Arun, Sarkar, Souvik, Schaeffer, Cynthia, Schattenberg, Jörn, Schiefke, Ingolf, Schiff, Eugene, Schmidt, Wolfgang, Schneider, Jeffrey, Schouten, Jeoffrey, Schultz, Michael, Sebastiani, Giada, Semela, David, Sepe, Thomas, Sheikh, Aasim, Sheikh, Muhammad, Sheridan, David, Sherman, Kenneth, Shibolet, Oren, Shiffman, Mitchell, Siddique, Asma, Sieberhagen, Cyril, Sigal, Samuel, Sikorska, Katarzyna, Simon, Krzysztof, Sinclair, Marie, Skoien, Richard, Solis, Joel, Sood, Siddharth, Souder, Bob, Spivey, James, Stal, Per, Stinton, Laura, Strasser, Simone, Svorcan, Petar, Szabo, Gyongzi, Talal, Andrew, Tam, Edward, Tetri, Brent, Thuluvath, Paul, Tobias, Hillel, Tomasiewicz, Krzysztof, Torres, Dawn, Tran, Albert, Trauner, Michael, Trautwein, Christian, Trotter, James, Tsochatzis, Emanuel, Unitt, Esther, Vargas, Victor, Varkonyi, Istvan, Veitsman, Ella, Vespasiani Gentilucci, Umberto, Victor, David, Vierling, John, Vincent, Catherine, Vincze, Aron, von der Ohe, Manfred, Von Roenn, Natasha, Vuppalanchi, Raj, Waters, Michael, Watt, Kymberly, Wattacheril, Julia, Weltman, Martin, Wieland, Amanda, Wiener, Gregory, Williams A, Alonzo, Williams J, Jeffrey, Wilson, Jason, Yataco, Maria, Yoshida, Eric, Younes, Ziad, Yuan, Liyun, Zivony, Adam, Zogg, Donald, Zoller, Heinz, Zoulim, Fabien, Zuckerman, Eli, and Zuin, Massimo

Abstract

Non-alcoholic steatohepatitis (NASH) is a common type of chronic liver disease that can lead to cirrhosis. Obeticholic acid, a farnesoid X receptor agonist, has been shown to improve the histological features of NASH. Here we report results from a planned interim analysis of an ongoing, phase 3 study of obeticholic acid for NASH.

Published
2019
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18. Pharmacological Therapy of Vascular Malformations of the Gastrointestinal Tract

Author

Szilagyi, Andrew and Ghali, Maged P

Subjects
Article Subject
Abstract

Vascular malformation (AVM) in the gastrointestinal tract is an uncommon, but not rare, cause of bleeding and iron deficiency anemia, especially in an aging population. While endoscopic coagulative therapy is the method of choice for controlling bleeding, a substantial number of cases require additional therapy. Adjunctive or even primary phamacotherapy may be indicated in recurrent bleeding. However, there is little evidence-based proof of efficacy for any agent. The bulk of support is derived from anecdotal reports or case series. The present review compares the outcome of AVM after no intervention, coagulative therapy or focus on pharmacological agents. Most of the literature encompasses two common AVMs, angiodysplasia and hereditary hemorrhagic telangiectasia. Similarly, the bulk of information evaluates two therapies, hormones (estrogen and progesterone) and the somatostatin analogue octreotide. Of these, the former is the only therapy evaluated in randomized trials, and the results are conflicting without clear guidelines. The latter therapy has been reported only as case reports and case series without prospective trials. In addition, other anecdotally used medications are discussed.

Published
2006
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