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20 results on '"Ghaderi, Rainak"'

1. A very French connection, a brief discourse in commemoration of life and services of the late Louis Pierre Joseph Delpy on his 120th birthday anniversary

Author

Tadayon, Keyvan, Mostafavi, Ehsan, Hajizadeh, Afshin, Ghaderi, Rainak, and Parham, Parham

Subjects
Archives of Razi Institute, Louis Pierre Joseph Delpy, Original Article, Iran, World Health Organization, Vaccine
Abstract

Razi Vaccine and Serum Research Institute (RVSRI) turned 95 years old in 2015. Majority of the animal infectious diseases such as rinderpest and anthrax that used to frequently strike the historic Persia are now gone for good or under control owing to the pioneering researches conducted at the institute in the early-mid 20th century in the field of vaccine manufacturing. The earliest such scientific contributions, were truly made by the French eminent veterinarian Dr. Louis Pierre Joseph Delpy who joined the institute in 1931. In his 18 year-long directorship tenure he taught his colleagues fundamentals of vaccinology, basics of modern epidemiology, essentials of infectious disease control disciplines, the art of scientific writing and much more things that changed the institute for ever. This paper reviews the events and turning points in the first 25 years of service of the institute in a chronological way and remarks Delpy’s principle involvements in all of these on the occasion of the 120 anniversary of his birth. At the entrance of the institute headquarter building where his bronze bust is placed, visitors can see a memorial etched plate that reads “... The architect of Razi and founder of Archives De L’Institute Razi (Archives of Razi Institute) was an enthusiastic scientist with a creative mind. … For the Razi community, Dr Delpy is gone but not forgotten.”

Published
2019

3. 'In-house' production of DNA size marker from a vaccinal Bacillus anthracis strain

Author

Sekhavati, Mohammad, Tadayon, Keyvan, Ghaderi, Rainak, Banihashemi, Reza, Jabbari, Ahmad Reza, Shokri, Gholamreza, and Karimnasab, Nasim

Subjects
PCR, lcsh:QR1-502, Original Article, sequencing, health care economics and organizations, Bacillus anthracis Sterne 34F2, lcsh:Microbiology, DNA size marker
Abstract

Background and Objectives: DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. Materials and Methods: Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce all the 10 fragments of a 100 bp DNA size marker. Results and Conclusion: The unpurified amalgam of 10 PCR products can be directly loaded to agarose gels. This work was intended to develop a reasonably cost-effective DNA ladder that is useful for researchers in laboratories with limited funding.

Published
2015

5. Pest rodents as the essential elements of Mycobacterium bovis controlling programs

Author

Moradi, Elahe, primary, Mosavari, Nader, additional, Tebianian, Majid, additional, Tadayon, Keyvan, additional, Ghaderi, Rainak, additional, Soleymani Babadi, Kioomars, additional, Mohammad Taheri, Mohammad, additional, Dashtipour, Shojaat, additional, Loni, Rahman, additional, Moradi Garavand, Morad, additional, Keshavarz, Pirooz, additional, Azarvandi, Alireza, additional, and Aref Pajoohi, Reza, additional

Published
2015
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7. Development and optimization of an ESAT6-ELISA-based detection system of Mycobacterium tuberculosis complex, suitable for bovine TB eradication

Author

Keshavarz, Rouhollah, primary, Mosavari, Nader, additional, Tadayon, Keyvan, additional, Fardid, Farzaneh, additional, Ghaderi, Rainak, additional, Babadi, Kioomars Soleymani, additional, Arefpajoohi, Reza, additional, Sajadi, Sayyed Hassan, additional, Taheri, Mohammad Mohammad, additional, Dashtipour, Shojaat, additional, Dehghanpour, Mahdi, additional, and Magami, Shamseddin Ghaem, additional

Published
2015
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8. Isolation, identification and molecular characterization of Mycobacterium bovis from tuberculin-positive cattle in Hamedan province of Iran

Author

Hatami, Samaneh, primary, Mosavari, Nader, additional, Soleymani Babadi, Kioomars, additional, Tadayon, Keyvan, additional, Ghaderi, Rainak, additional, Keshavarz, Roholah, additional, Arefpajoohi, Reza, additional, Mohammad Taheri, Mohammad, additional, Hassan Sajadi, Sayyed, additional, Reza Azimi, Mohamad, additional, Dashtipour, Shojaat, additional, Moradi Garavand, Morad, additional, and Bromandfar, Samed, additional

Published
2015
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9. Investigation of Mycobacterium bovis population by RFLP scheme, in two main provinces of Iran – Tehran and Alborz

Author

Arefpajoohi, Reza, primary, Mosavari, Nader, additional, Mirhashemi, Simin, additional, Tadayon, Keyvan, additional, Ghaderi, Rainak, additional, Keshavarz, Rohollah, additional, Soleymani Babadi, Kioomars, additional, Mohammad Taheri, Mohammad, additional, Hassan Sajadi, Sayyed, additional, Dashtipour, Shojaat, additional, Moradi Garavand, Morad, additional, and Assar, Masoud, additional

Published
2015
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10. Study of Mycobacterium bovis genotypes in human and bovine isolates using spoligotyping, MIRUVNTR and RFLP-PCR

Author

Khaleghian, Pooyan, primary, Farnia, Parisa, additional, Tadayon, Keivan, additional, Mosavari, Nader, additional, Mozafari, Mohadeseh, additional, Derakhshani Nejad, Zahra, additional, Keshavarz, Rouollah, additional, Dashtipour, Shojaat, additional, Masjedi, Mohamad Reza, additional, Vellayati, Ali Akbar, additional, Ghaderi, Rainak, additional, and Boroumandfar, Samed, additional

Published
2015
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11. Iranian clonal population of Salmonella enterica serovar Enteritidis, characterized by multi-locus sequence typing (MLST) method.

Author

Ghaderi, Rainak, Tadayon, Keyvan, Khaki, Pejvak, and Mosavari, Nader

Subjects
*SALMONELLA enterica serovar enteritidis, *SALMONELLA enterica, *SALMONELLA diseases, *MOLECULAR epidemiology, *CHICKEN diseases
Abstract

Background and Objectives: Some 2 million tons of chicken meat is produced by Iran per annum, positioning Iran among the top producers in the region. This study aimed to evaluate the molecular epidemiology and genetic characteristics of Salmonella enterica Enteritidis in Iran. Materials and Methods: A representative selection of isolates (n=76), initially genotyped by a 7-locus MLVA typing system, was examined by the standard MLST genotyping. Results and Conclusion: All the MLVA typed isolates, classified into six types, were gathered under a single ST11 MLST type. This is an intriguing observation as much more genome heterogeneity was expected considering the extent of diversity in the host and geography origin of the examined isolates. ST11, on the other hand is not exclusively found in Iran as it is reported also from Brazil, Denmark, Japan and the United States. In explanation of these observations, ST11 might stand for a single probably ancestral clone of Salmonella enterica Enteritidis successfully scattered in all these geographically diverse countries. Further global investigation covering more isolates and methods like whole genome sequencing would be advisable. [ABSTRACT FROM AUTHOR]

Published
2015

12. The population structure of Salmonella enterica Enteritidis in Iran analyzed by multiple-locus variable-number tandem repeat analysis

Author

Ghaderi, Rainak, primary, Tadayon, Keyvan, additional, Avagyan, Sargis, additional, Khaki, Pejvak, additional, Bidhendi, Soheila Moradi, additional, Forbes, Ken James, additional, Mosavari, Nader, additional, Toroghi, Mohammad Reza, additional, Moosakhani, Farhad, additional, Banihashemi, Reza, additional, Sekhavati, Mohamad, additional, and Karimnasab, Nasim, additional

Published
2012
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13. Genetic Characterization of Bacillus anthracis 17 JB strain.

Author

Seyed-Mohamadi, Sakineh, Bidhendi, Soheila Moradi, Tadayon, Keyvan, and Ghaderi, Rainak

Subjects
BACILLUS anthracis, GENETICS, ANTHRAX vaccines, EPIDEMIOLOGY, VACCINE manufacturing
Abstract

Background and Objectives: Bacillus anthracis is one of the most homogenous bacteria ever described. Some level of diversity. Bacillus anthracis 17JB is a laboratory strain It is broadly used as a challenge strain in guinea pigs for potency test of anthrax vaccine. Material and Methods: This work describes genetic characterization of B. anthracis 17 JB strain using the SNPs and MLVA genotyping. Results and Conclusion: In SNPs typing, the originally French 17JB strain represented the A.Br. 008/009 subgroup. In Levy's genotyping method, 843, 451 and 864 bp long fragments were identified at AA03, AJ03 and AA07 loci, respectively. In the vaccine manufacturer perspective these findings are much valuable on their own account, but similar research is required to extend molecular knowledge of B. anthracis epidemiology in Persia. [ABSTRACT FROM AUTHOR]

Published
2015

15. Investigation of Mycobacterium bovispopulation by RFLP scheme, in two main provinces of Iran – Tehran and Alborz

Author

Arefpajoohi, Reza, Mosavari, Nader, Mirhashemi, Simin, Tadayon, Keyvan, Ghaderi, Rainak, Keshavarz, Rohollah, Soleymani Babadi, Kioomars, Mohammad Taheri, Mohammad, Hassan Sajadi, Sayyed, Dashtipour, Shojaat, Moradi Garavand, Morad, and Assar, Masoud

Abstract

Bovine tuberculosis (BTB) caused by Mycobacterium bovisis considered as one of the most important diseases of cattle. Identifying and culling infected animals following positive tuberculin test detection is one of the fundamental control strategies of tuberculosis in cattle in the world. Passing five decades after implementation of such a control program from 1963, now, Iran experiences an admirable drastic decreased rate of 0.18% in comparison with a previous 5% at the beginning of the program.

Published
2015
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16. Isolation, identification and molecular characterization of Mycobacterium bovisfrom tuberculin-positive cattle in Hamedan province of Iran

Author

Hatami, Samaneh, Mosavari, Nader, Soleymani Babadi, Kioomars, Tadayon, Keyvan, Ghaderi, Rainak, Keshavarz, Roholah, Arefpajoohi, Reza, Mohammad Taheri, Mohammad, Hassan Sajadi, Sayyed, Reza Azimi, Mohamad, Dashtipour, Shojaat, Moradi Garavand, Morad, and Bromandfar, Samed

Abstract

Tuberculosis (TB) remains a major cause of death in many countries. Bovine tuberculosis caused by Mycobacterium bovisis one of the key members of the Mycobacterium tuberculosiscomplex. Current methods need long incubation times, which is in contrast to the necessity for rapid and accurate identification and isolation. Molecular techniques, such as RFLP fingerprinting, for accurate identification and differentiation of M. tuberculosisisolates are a more desirable approach.

Published
2015
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17. Pest rodents as the essential elements of Mycobacterium boviscontrolling programs

Author

Moradi, Elahe, Mosavari, Nader, Tebianian, Majid, Tadayon, Keyvan, Ghaderi, Rainak, Soleymani Babadi, Kioomars, Mohammad Taheri, Mohammad, Dashtipour, Shojaat, Loni, Rahman, Moradi Garavand, Morad, Keshavarz, Pirooz, Azarvandi, Alireza, and Aref Pajoohi, Reza

Abstract

As a rule in any Bovine tuberculosis (BTB) control program, recognition of infection source is of great importance. Hence in this study, isolation and evaluation of Mycobacterial genomic patterns obtained from rodents of infected farms by RFLP method was conducted.

Published
2015
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18. Study of Mycobacterium bovisgenotypes in human and bovine isolates using spoligotyping, MIRUVNTR and RFLP-PCR

Author

Khaleghian, Pooyan, Farnia, Parisa, Tadayon, Keivan, Mosavari, Nader, Mozafari, Mohadeseh, Derakhshani Nejad, Zahra, Keshavarz, Rouollah, Dashtipour, Shojaat, Masjedi, Mohamad Reza, Vellayati, Ali Akbar, Ghaderi, Rainak, and Boroumandfar, Samed

Abstract

The aim of this study is to investigate and detect the prevalence of Mycobacterium bovissubtypes (Mycobacterium bovissubtype bovisand Mycobacterium bovissubtype caprae) in humans and compare the genetic diversity of Mycobacterium bovisin humans and cattle with spoligotyping methods, as well as pyrazinamide susceptibility study of subtypes.

Published
2015
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19. Development and optimization of an ESAT6-ELISA-based detection system of Mycobacterium tuberculosiscomplex, suitable for bovine TB eradication

Author

Keshavarz, Rouhollah, Mosavari, Nader, Tadayon, Keyvan, Fardid, Farzaneh, Ghaderi, Rainak, Babadi, Kioomars Soleymani, Arefpajoohi, Reza, Sajadi, Sayyed Hassan, Taheri, Mohammad Mohammad, Dashtipour, Shojaat, Dehghanpour, Mahdi, and Magami, Shamseddin Ghaem

Abstract

The tuberculin test is the most common method used for the detection and control of bovine tuberculosis (TB). Specificity and sensitivity of this tuberculin test is low, so it is better to perform another test in parallel for investigation of the disease. The ELISA test is user friendly, so it can be an appropriate test for performing in parallel with the tuberculin test for an accurate detection. The aim of this study is the development of an ELISA test (kit) based on proteins smaller than 10kDa (specially ESAT-6) for the detection of TB in cattle.

Published
2015
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20. A very French connection, a brief discourse in commemoration of life and services of the late Louis Pierre Joseph Delpy on his 120 th birthday anniversary.

Author

Tadayon K, Mostafavi E, Hajizadeh A, Ghaderi R, and Parham P

Abstract

Razi Vaccine and Serum Research Institute (RVSRI) turned 95 years old in 2015. Majority of the animal infectious diseases such as rinderpest and anthrax that used to frequently strike the historic Persia are now gone for good or under control owing to the pioneering researches conducted at the institute in the early-mid 20 th century in the field of vaccine manufacturing. The earliest such scientific contributions, were truly made by the French eminent veterinarian Dr. Louis Pierre Joseph Delpy who joined the institute in 1931. In his 18 year-long directorship tenure he taught his colleagues fundamentals of vaccinology, basics of modern epidemiology, essentials of infectious disease control disciplines, the art of scientific writing and much more things that changed the institute for ever. This paper reviews the events and turning points in the first 25 years of service of the institute in a chronological way and remarks Delpy's principle involvements in all of these on the occasion of the 120 anniversary of his birth. At the entrance of the institute headquarter building where his bronze bust is placed, visitors can see a memorial etched plate that reads " ... The architect of Razi and founder of Archives De L'Institute Razi (Archives of Razi Institute) was an enthusiastic scientist with a creative mind. … For the Razi community, Dr Delpy is gone but not forgotten.", (© 2019 Urmia University. All rights reserved.)

Published
2019
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