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We hypothesize that melanocortin receptors (MC) could activate tissue protective circuit in a model of streptozotocin- (STZ-) induced diabetic retinopathy (DR) in mice. At 12–16 weeks after diabetes induction, fluorescein angiography (FAG) revealed an approximate incidence of 80% microvascular changes, typical of DR, in the animals, without signs of vascular leakage. Occludin progressively decreased in the retina of mice developing retinopathy. qPCR of murine retina revealed expression of two MC receptors, Mc1r and Mc5r. The intravitreal injection (5 μL) of the selective MC1 small molecule agonist BMS-470539 (33 μmol) and the MC5 peptidomimetic agonist PG-901 (7.32 nM) elicited significant protection with regular course and caliber of retinal vessels, as quantified at weeks 12 and 16 after diabetes induction. Mouse retina homogenate settings indicated an augmented release of IL-1α, IL-1β, IL-6, MIP-1α, MIP-2α, MIP-3α, and VEGF from diabetic compared to nondiabetic mice. Application of PG20N or AGRP and MC5 and MC1 antagonist, respectively, augmented the release of cytokines, while the agonists BMS-470539 and PG-901 almost restored normal pattern of these mediators back to nondiabetic values. Similar changes were quantified with respect to Ki-67 staining. Finally, application of MC3-MC4 agonist/antagonists resulted to be inactive with respect to all parameters under assessment. [ABSTRACT FROM AUTHOR]

In the article titled "Activation of Melanocortin Receptors MC1 and MC5 Attenuates Retinal Damage in Experimental Diabetic Retinopathy" [[1]], an error was identified in Figure 3 as raised on PubPeer [[2]]. Activation of Melanocortin Receptors MC1 and MC5 Attenuates Retinal Damage in Experimental Diabetic Retinopathy; PubPeer, January 2020, https://pubpeer.com/publications/40F965407B51FE7296E59EBD9A0791. [Extracted from the article]

Introduction: The purine analog 6-thioguanine (6TG), an old drug approved in the 60s to treat acute myeloid leukemia (AML), was tested in the diabetic retinopathy (DR) experimental in vivo setting along with a molecular modeling approach., Methods: A computational analysis was performed to investigate the interaction of 6TG with MC1R and MC5R. This was confirmed in human umbilical vein endothelial cells (HUVECs) exposed to high glucose (25 mM) for 24 h. Cell viability in HUVECs exposed to high glucose and treated with 6TG (0.05-0.5-5 µM) was performed. To assess tube formation, HUVECs were treated for 24 h with 6TG 5 µM and AGRP (0.5-1-5 µM) or PG20N (0.5-1-5-10 µM), which are MC1R and MC5R antagonists, respectively. For the in vivo DR setting, diabetes was induced in C57BL/6J mice through a single streptozotocin (STZ) injection. After 2, 6, and 10 weeks, diabetic and control mice received 6TG intravitreally (0.5-1-2.5 mg/kg) alone or in combination with AGRP or PG20N. Fluorescein angiography (FA) was performed after 4 and 14 weeks after the onset of diabetes. After 14 weeks, mice were euthanized, and immunohistochemical analysis was performed to assess retinal levels of CD34, a marker of endothelial progenitor cell formation during neo-angiogenesis., Results: The computational analysis evidenced a more stable binding of 6TG binding at MC5R than MC1R. This was confirmed by the tube formation assay in HUVECs exposed to high glucose. Indeed, the anti-angiogenic activity of 6TG was eradicated by a higher dose of the MC5R antagonist PG20N (10 µM) compared to the MC1R antagonist AGRP (5 µM). The retinal anti-angiogenic effect of 6TG was evident also in diabetic mice, showing a reduction in retinal vascular alterations by FA analysis. This effect was not observed in diabetic mice receiving 6TG in combination with AGRP or PG20N. Accordingly, retinal CD34 staining was reduced in diabetic mice treated with 6TG. Conversely, it was not decreased in diabetic mice receiving 6TG combined with AGRP or PG20N., Conclusion: 6TG evidenced a marked anti-angiogenic activity in HUVECs exposed to high glucose and in mice with DR. This seems to be mediated by MC1R and MC5R retinal receptors., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Trotta, Gesualdo, Lepre, Russo, Ferraraccio, Panarese, Marano, Grieco, Petrillo, Hermenean, Simonelli, D’Amico, Bucolo, Lazzara, De Nigris, Rossi and Platania.)

Background and Objectives : The role and the levels of ghrelin in diabetes-induced retinal damage have not yet been explored. The present study aimed to measure the serum levels of total ghrelin (TG), and its acylated (AG) and des-acylated (DAG) forms in patients with the two stages of diabetic retinopathy (DR), non-proliferative (NPDR) and proliferative (PDR). Moreover, the correlation between serum ghrelin and neutrophil elastase (NE) levels was investigated. Materials and Methods : The serum markers were determined via enzyme-linked immunosorbent assays in 12 non-diabetic subjects (CTRL), 15 diabetic patients without DR (Diabetic), 15 patients with NPDR, and 15 patients with PDR. Results : TG and AG serum levels were significantly decreased in Diabetic (respectively, p < 0.05 and p < 0.01 vs. CTRL), NPDR ( p < 0.01 vs. Diabetic), and in PDR patients ( p < 0.01 vs. NPDR). AG serum levels were inversely associated with DR abnormalities (microhemorrhages, microaneurysms, and exudates) progression (r = -0.83, p < 0.01), serum neutrophil percentage (r = -0.74, p < 0.01), and serum NE levels (r = -0.73, p < 0.01). The latter were significantly increased in the Diabetic ( p < 0.05 vs. CTRL), NPDR ( p < 0.01 vs. Diabetic), and PDR ( p < 0.01 vs. PDR) groups. Conclusions : The two DR stages were characterized by decreased AG and increased NE levels. In particular, serum AG levels were lower in PDR compared to NPDR patients, and serum NE levels were higher in the PDR vs. the NPDR group. Together with the greater presence of retinal abnormalities, this could underline a distinctive role of AG in PDR compared to NPDR.

Background: End-of-life (EOL) communication is often avoided, especially among young adults. Negative expectations concerning EOL conversations with relatives or significant others are one major reason., Objective: To investigate how best to violate negative expectations concerning EOL conversations by identifying predictors of coping with expectation violations in this context., Methods: Vignettes describing expectation violations in the context of EOL communication were presented to a sample of 261 university students. In a first experiment, the credibility of the expectation-disconfirming information was manipulated. In a second experiment, the valence of the disconfirming evidence was manipulated. As outcome measures, the subjective likelihood of two different responses to the expectation violation was assessed: (1) ignoring the disconfirming evidence (immunization) and (2) changing expectations (accommodation)., Results: Overall, participants experiencing a worse-than-expected event showed more immunization [ F (1, 257) = 12.15, p  < 0.001, η p  = 0.05], while participants experiencing a better-than-expected event showed more accommodation [ F (1, 257) = 30.98, p  < 0.001, η p  = 0.11]. Participants with higher fear of death [ F (1, 257) = 12.24, p  < 0.001, η p  = 0.05] as well as higher death avoidance tendencies [ F (1, 257) = 17.16, p  < 0.001, η p  = 0.06] showed less accommodation in response to a better-than-expected event., Conclusion: In general, young adults appear to update their expectations quickly in response to unexpectedly positive experiences in the context of EOL communication. However, individuals with higher fear of death and higher death avoidance tendencies appear to be at higher risk of maintaining negative expectations despite disconfirming evidence., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Bendel, Gesualdo, Pinquart and von Blanckenburg.)

Background: To perform a multimodal assessment of the ectopic inner foveal layers' (EIFL) prognostic role on idiopathic epiretinal membrane (ERM) surgery., Methods: We retrospectively followed-up for 12 months 27 patients who underwent ERM surgery and stratified them based on EIFL presence (group 1) or absence (group 2) at baseline. Central Retinal Thickness (CRT) and best-corrected visual acuity (BCVA) were compared pre- and post-operatively at 1, 4 and 12 months, whereas fixation stability (FS), macular sensitivity (MS) and multifocal electroretinogram (mfERG) responses were confronted at baseline and 12 months., Results: In group 1, BCVA improved at 4 and 12 months (MD = 0.14 (SE = 0.04); MD = 0.13 (SE = 0.05), respectively) as well as in group 2 (MD = 0.31 (SE = 0.07); MD = 0.41 (SE = 0.08), respectively). CRT did not change in group 1, whereas it decreased in group 2 at 4 and 12 months (MD = -73.13; SE = 23.56; MD = -76.20; SE = 23.56). MS showed no changes in both groups after surgery. FS did not change in group 1, whereas group 2 improved FS 2° (+8.91 ± 13.97) and FS 4° (+4.33 ± 3.84). MfERG P1 wave did not change in group 1, while in group 2 αP1-2, αP1-3 and αP1-4 improved postoperatively (27.97 ± 27.62; 12.51 ± 17.36; 10.49 ± 17.19, respectively)., Conclusions: Multimodal assessment confirmed that EIFL negatively affected ERM surgery outcomes.

Background: Underprivileged youth in the Dominican Republic (DR) are at high risk of acquiring the human immunodeficiency virus (HIV). Protective parenting practices may inhibit sexual risk-taking., Objective: We investigated whether parental involvement in a sports-based HIV prevention program increased self-efficacy to prevent HIV and safe sex behavior among Dominican youth., Method: The study had a quasi-experimental design with repeated measures. N = 90 participants between 13 and 24 years of age participated in the program through two different trainings, UNICA and A Ganar, both of which had an experimental (i.e., program with parental component) and a control (i.e., program without parental component) condition., Results: Self-efficacy to prevent HIV significantly increased among participants in the experimental condition of UNICA. Self-efficacy for safe sex increased among sexually active participants in the experimental condition of A Ganar. Implications for Impact: These findings are important to meet the United Nations' Sustainable Development Goal of good health and wellbeing, as they suggest that parental involvement in sports-based HIV prevention programs can enhance their positive effects for increasing youth's self-efficacy to practice HIV-preventive behaviors. Randomized control trials and longitudinal studies are needed.

Background and Objectives: To report the real-life Brolucizumab therapeutical outcomes of treatment-naïve and non-treatment-naïve eyes with neovascular age-related macular degeneration (nAMD) and to analyze the incidence of therapy-related adverse events. Materials and Methods: A total of 56 eyes of 54 patients diagnosed with nAMD were retrospectively evaluated over a 3-month follow-up. Naïve eyes received a 3-month loading phase, whereas non-naïve eyes were treated with one intravitreal injection + ProReNata scheme. The main outcome measures were best-corrected visual acuity (BCVA) and central retinal thickness (CRT) change. In addition, patients were stratified on the basis of fluid accumulation site, whether intra-retinal (IRF), sub-retinal (SRF), or sub-retinal pigmented epithelium (SRPE), to separately assess the eventual BCVA change in each subgroup. Finally, the incidence of ocular adverse events was evaluated. Results: In naïve eyes, a significant improvement of BCVA (LogMar) was observed at all timepoints from baseline (1 month-Mean Difference (MD): -0.13; 2 months MD: -0.17; 3 months MD: -0.24). In non-naïve eyes, a significant mean change was observed at all timepoints, with the exception of 1-month follow-up (2 months MD: -0.08; 3 months MD: -0.05). CRT significantly changed in both groups at all timepoints at a similar pace within the first two months, with naïve eyes displaying a larger overall thickness decrease at the end of the follow-up (Group 1 = MD: -123.91 µm; Group 2 = MD: -110.33 µm). With respect to the location of the edema, a significant BCVA change was observed in naïve patients with fluid in all three sites at the end of the follow-up (SRPE = MD: -0.13 ( p = 0.043); SR = MD: -0.15 ( p = 0.019); IR = MD: -0.19 ( p = 0.041). Non-naïve patients exhibited significant mean BCVA changes only with respect to SR and IR fluid presence (SRPE = MD: -0.13 ( p = 0.152); SR = MD: -0.15 ( p = 0.007); IR = MD: -0.06 ( p = 0.011). One naïve patient experienced acute-onset anterior and intermediate uveitis which completely resolved after therapy. Conclusions: Brolucizumab was demonstrated to be a safe and efficient alternative in improving both the anatomical and functional parameters of eyes with nAMD in this small, uncontrolled, series of patients.

Diabetic retinopathy (DR) is the most frequent microvascular retinal complication of diabetic patients, contributing to loss of vision. Recently, retinal neuroinflammation and neurodegeneration have emerged as key players in DR progression, and therefore, this review examines the neuroinflammatory molecular basis of DR. We focus on four important aspects of retinal neuroinflammation: (i) the exacerbation of endoplasmic reticulum (ER) stress; (ii) the activation of the NLRP3 inflammasome; (iii) the role of galectins; and (iv) the activation of purinergic 2X7 receptor (P2X7R). Moreover, this review proposes the selective inhibition of galectins and the P2X7R as a potential pharmacological approach to prevent the progression of DR.

Objective: in Colombia, many first-year university students consume unhealthy food, are physically inactive, and drink regularly, which can be associated with the behavior of social ties, living with social ties, and time with peers. The present cross-sectional study assessed the association between health behaviors of first-semester students and these factors., Method: N = 189 ( M age = 18.79; SD = 1.07; female = 68.8%) first-semester students in Colombia completed an online questionnaire investigating current and expected health behaviors as well as influencing factors. ANCOVAs, bivariate correlations, moderation analyses, and hierarchical regressions were used to analyze the data., Results: expected food consumption (stronger among participants who live with parents) as well as current and expected heavy drinking and binge drinking (stronger among participants who do not live with parents) were significantly correlated to the respective parental behavior. Current and expected drinking was significantly correlated to partners' drinking. Expected physical activity was correlated with peers' physical activity. Partners' attempts to encourage drinking moderated the association between participants' current and expected drinking with partners' drinking. Time spent with peers was related to heavy drinking and engaging in more physical activity., Conclusion: in Colombia, parents appear to play a significant role in their offspring's health behaviors during their first semester at university, particularly regarding food consumption and alcohol use. Partners' drinking and time spent with peers are strongly related to heavy drinking.

Background: Vitamin D deficiency has been associated with dry eye development during Sjögren's syndrome (SS). Here, we investigated whether repeated oral vitamin D3 supplementation could prevent the corneal epithelium damage in an SS mouse model., Methods: 30 female mouse knock-out for the thrombospondin 1 gene were randomized (six per group) in untreated mice euthanized at 6 weeks as negative control (C-) or at 12 weeks as the positive control for dry eye (C+). Other mice were sacrificed after 6 weeks of oral vitamin D3 supplementation in the drinking water (1000, 8000, and 20,000 IU/kg/week, respectively)., Results: The C+ mice showed alterations in their corneal epithelial morphologies and thicknesses ( p < 0.01 vs. C-), while the mice receiving 8000 (M) and 20,000 (H) IU/kg/week of vitamin D3 showed preservation of the corneal epithelium morphology and thickness ( p < 0.01 vs. C+). Moreover, while the C+ mice exhibited high levels and activity of corneal tumor necrosis factor alpha converting enzyme (TACE), neovascularization and fibrosis markers; these were all reduced in the M and H mice., Conclusions: Oral vitamin D3 supplementation appeared to counteract the negative effect of TACE on corneal epithelium in a mouse model of SS-associated dry eye.

Background: First-year students often adopt health risk behaviors during their first semester such as increased consumption of unhealthy food, decreased physical activity, and increased alcohol use. Expectations, social tie's efforts to motivate behavior, and coresidence with parents can influence said behaviors., Aims: We assessed how students' health behaviors and expectations change over the first semester, and how the aforementioned factors influence the maintenance or change of behavior and expectations., Methods: A longitudinal survey design was implemented. A total of N  = 163 German first-year students (81% female; 18% male; 1% non-binary; M age  = 21.20, SD  = 2.66) completed online questionnaires, including the NCHRBS and AUDIT, during the Covid-19 pandemic at the beginning (November 2020) and after the end (May 2021) of their first semester., Results: Current and expected food consumption and physical activity became healthier over time. The current and expected number of drinks consumed per month increased. Change in expectations for physical activity, number of drinks and binge drinking were predicted by the initial respective behavior. The number of drinks and expected physical activity became unhealthier in relation to reported initial parental influence to drink and to be physically inactive. Moving out of the parental home predicted an increase in current and expected number of drinks and in current and expected binge drinking. These effects of moving out were not mediated by perceived parental or peer influence., Conclusions: Interventions should target these behaviors and expectations during the first semester and address parental influence on physical activity and alcohol use., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

Background: Diabetic retinopathy (DR) is a neurovascular disease, characterized by a deficiency of brain-derived neurotrophic factor (BDNF), a regulator of autophagy. Beta-hydroxybutyrate (BHB), previously reported as a protective agent in DR, has been associated with BDNF promotion. Here, we investigated whether systemic BHB affects the retinal levels of BDNF and local autophagy in diabetic mice with retinopathy; Methods: C57BL/6J mice were administered with intraperitoneal (i.p.) streptozotocin (STZ) (75 mg/kg) injection to develop diabetes. After 2 weeks, they received i.p. injections of BHB (25−50−100 mg/kg) twice a week for 10 weeks. Retinal samples were collected in order to perform immunofluorescence, Western blotting, and ELISA analysis; Results: BHB 50 mg/kg and 100 mg/kg significantly improved retinal BDNF levels (p < 0.01) in diabetic mice. This improvement was negatively associated with autophagosome−lysosome formations (marked by LC3B and ATG14) and to higher levels of connexin 43 (p < 0.01), a marker of cell integrity. Moreover, BHB administration significantly reduced M1 microglial activation and autophagy (p < 0.01); Conclusions: The systemic administration of BHB in mice with DR improves the retinal levels of BDNF, with the consequent reduction of the abnormal microglial autophagy. This leads to retinal cell safety through connexin 43 restoration., Competing Interests: The authors declare no conflict of interest.

Objectives: Individuals often experience expectation violations related to the consumption of healthy food and physical activity and they may cope with expectation-disconfirming information by (1) ignoring the discrepancy (immunization), (2) increasing efforts to fulfill them (assimilation), or (3) changing their expectations (accommodation). We investigated whether valence, discrepancy magnitude, and controllability of the expectation disconfirming event predicted coping with expectation violations. Methods: A 2 (valence: positive vs negative) x 2 (discrepancy: larger vs smaller) x 2 (controllability: control vs no control) experimental design was implemented. Overall, we presented 297 university students with vignettes describing expectation violations and present different combinations of predictor levels. Results: Regarding physical activity, participants showed significantly higher accommodation when experiencing a better-than-expected event and showed significantly higher immunization when experiencing a worse-than-expected event. Regarding food consumption and physical activity, individuals experiencing lower discrepancy showed significantly higher immunization; individuals with control over the source of expectation disconfirmation showed significantly higher assimilation; and individuals without control over the source of expectation disconfirmation showed significantly higher accommodation. Conclusions: To promote the maintenance of healthy expectations, despite expectation violations, interventions could foster the perception of control as well as assimilative behavior.

Diabetic retinopathy (DR) is a neurovascular disease characterized by the reduction of retina integrity and functionality, as a consequence of retinal pigment epithelial cell fibrosis. Although galectin-1 (a glycan-binding protein) has been associated with dysregulated retinal angiogenesis, no evidence has been reported about galectin-1 roles in DR-induced fibrosis. ARPE-19 cells were cultured in normal (5 mM) or high glucose (35 mM) for 3 days, then exposed to the selective galectin-1 inhibitor OTX008 (2.5-5-10 μM) for 6 days. The determination of cell viability and ROS content along with the analysis of specific proteins (by immunocytochemistry, Western blotting, and ELISA) or mRNAs (by real time-PCR) were performed. OTX008 5 μM and 10 μM improved cell viability and markedly reduced galectin-1 protein expression in cells exposed to high glucose. This was paralleled by a down-regulation of the TGF-β/, NF-kB p65 levels, and ROS content. Moreover, epithelial-mesenchymal transition markers were reduced by OTX008 5 μM and 10 μM. The inhibition of galectin-1 by OTX008 in DR may preserve retinal pigment epithelial cell integrity and functionality by reducing their pro-fibrotic phenotype and epithelial-mesenchymal transition phenomenon induced by diabetes.

Objective: Alcohol expectancies are a critical factor in the development of problematic alcohol use. Expectancy challenge (EC) interventions aim to manipulate positive alcohol expectancies to reduce or prevent alcohol use among young people. The present meta-analysis investigated the effects of ECs at changing expectations and alcohol use among high school and college students, and moderating effects of study and individual characteristics on these changes., Method: A total of 23 EC studies (N = 4,122; mean age = 19.0; 57% males) was included as they reported enough information to calculate effect sizes, had a control condition that did not receive an active intervention, and were presented as of August 1, 2020. Two independent coders coded relevant variables and calculated effect sizes at posttest using a random-effects model., Results: ECs showed significant yet small effects at modifying alcohol consumption and alcohol expectancies in the desired direction (g's ranged from -.18 to -.42). Changes in social, tension, liquid courage, and risk aggression expectancies explained significant variance in change in alcohol use. The effects of ECs at changing social, sexual, tension, and liquid courage expectancies were stronger among college students compared to high school students. More favorable results were observed for interventions delivered at a higher dose., Conclusions: ECs targeting high school and college students produce small effects at reducing alcohol use and changing alcohol expectancies. Future efforts are needed to determine under which circumstances and among which subgroups ECs are expected to produce greater effects. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

Split intein-mediated protein trans- splicing expands AAV transfer capacity, thus overcoming the limited AAV cargo. However, non-mammalian inteins persist as trans- splicing by-products, and this could raise safety concerns for AAV intein clinical applications. In this study, we tested the ability of several degrons to selectively decrease levels of inteins after protein trans- splicing and found that a version of E. coli dihydrofolate reductase, which we have shortened to better fit into the AAV vector, is the most effective. We show that subretinal administration of AAV intein armed with this short degron is both safe and effective in a mouse model of Stargardt disease (STGD1), which is the most common form of inherited macular degeneration in humans. This supports the use of optimized AAV intein for gene therapy of both STGD1 and other conditions that require transfer of large genes., Competing Interests: A.A., P.T., and I.T. are co-inventors on the patent application number PCT/EP2019/0708020 entitled “Intein proteins and uses thereof.” A.A. is founder, shareholder, and consultant of InnovaVector srl and AAVantgarde Bio. The other authors declare that they have no competing interests., (© 2021 The Authors.)

This study aimed to investigate the interactions between fingolimod, a sphingosine 1-phosphate receptor (S1PR) agonist, and melanocortin receptors 1 and 5 (MCR1, MCR5). In particular, we investigated the effects of fingolimod, a drug approved to treat relapsing-remitting multiple sclerosis, on retinal angiogenesis in a mouse model of diabetic retinopathy (DR). We showed, by a molecular modeling approach, that fingolimod can bind with good-predicted affinity to MC1R and MC5R. Thereafter, we investigated the fingolimod actions on retinal MC1Rs/MC5Rs in C57BL/6J mice. Diabetes was induced in C57BL/6J mice through streptozotocin injection. Diabetic and control C57BL/6J mice received fingolimod, by oral route, for 12 weeks and a monthly intravitreally injection of MC1R antagonist (AGRP), MC5R antagonist (PG20N), and the selective S1PR1 antagonist (Ex 26). Diabetic animals treated with fingolimod showed a decrease of retinal vascular endothelial growth factor A (VEGFA) and vascular endothelial growth factor receptors 1 and 2 (VEGFR1 and VEGFR2), compared to diabetic control group. Fingolimod co-treatment with MC1R and MC5R selective antagonists significantly ( p < 0.05) increased retinal VEGFR1, VEGFR2, and VEGFA levels compared to mice treated with fingolimod alone. Diabetic animals treated with fingolimod plus Ex 26 (S1PR1 selective blocker) had VEGFR1, VEGFR2, and VEGFA levels between diabetic mice group and the group of diabetic mice treated with fingolimod alone. This vascular protective effect of fingolimod, through activation of MC1R and MC5R, was evidenced also by fluorescein angiography in mice. Finally, molecular dynamic simulations showed a strong similarity between fingolimod and the MC1R agonist BMS-470539. In conclusion, the anti-angiogenic activity exerted by fingolimod in DR seems to be mediated not only through S1P1R, but also by melanocortin receptors., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Gesualdo, Balta, Platania, Trotta, Herman, Gharbia, Rosu, Petrillo, Giunta, Della Corte, Grieco, Bellavita, Simonelli, D’Amico, Hermenean, Rossi and Bucolo.)

Objective: The start of university is a critical period for health risk behavior (i.e. eating, physical activity, alcohol use) which can be influenced by expectations and by environmental factors such as living arrangement, health behaviors of close social ties (i.e. parents, partners, peers), and time spent with peers. We investigated associations between environmental factors and current/expected health behaviors of German freshmen during the COVID-19 pandemic., Method: A cross-sectional survey design was used. A total of N  = 208 students (82.7% female; M age = 20.90, SD  = 4.10) completed an online questionnaire assessing health behaviors and environmental factors at the beginning of their first semester., Results: Current and expected physical activity was associated to that of all social ties, current and expected alcohol use to partner's and peers' alcohol use, while current and expected eating was only associated to peers' eating. The relationship between partner's or peers' and participant's alcohol use was moderated by coresidence, with a greater probability of engaging in these behaviors observed in case of coresidence. Perceived peer encouragement for alcohol consumption moderated the relationship between peer alcohol use and the number of drinks consumed by participants per month. Participants who spend more time with peers were more likely to consume higher amounts of alcohol. No differences were found regarding present and expected behaviors of participants who moved out of their parents' home and those who did not., Conclusion: Partners and peers significantly influence students' health behaviors, particularly alcohol use. Interventions to prevent health risk behaviors among freshmen should therefore address these social ties' influence., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)