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Staphylococcal hemagglutinating antibodies are present in both 7S and 19S fractions of serum. Newly born infants receive only 7S hemagglutinins from their mothers, and this passively acquired antibody soon disappears from the circulation. Infants under 4 months of age fail to respond to severe staphylococcal infections with hemagglutinin production. Infants over 4 months of age do make hemagglutinins, but these antibodies are mostly in the 19S class. Infants and children over 4 months of age may have high titers of hemagglutinins even without a history of staphylococcal infections. Most adults have significant staphylococcal hemagglutinin titers. The distribution of staphylococcal hemagglutinating antibodies between 7S and 195 is consistent. Under the age of 16 years, most of the actively acquired hemagglutinin is in the 19S class. After 16, 7S hemagglutinins may dominate. Thus, under the conditions of natural exposure to staphylococci, age of the individual is a significant determinant of the class of his staphylococcal hemaglutinating antibodies. Although it is clear from this and other reported studies that individuals react to different antigenic stimuli by making different classes of antibodies, the biological and clinical significance of these observations are not yet completely understood.

Introduction Urinary sediment has been recognized for a long time as a useful parameter for the evaluation of health and disease. It is the purpose of this communication to describe and evaluate a simple quantitative method of examination which also permits careful and repeated study of the exfoliated cells. The method consists of the filtration of a measured amount of urine through a membrane of known surface area and of pore size such that all formed elements in the specimen are retained on the surface of the membrane. The formed elements are fixed and stained on this filter membrane which subsequently is rendered transparent. The cells and casts are examined by means of a light microscope and counted. The results are expressed as the number of formed elements per milliliter of urine. Materials and Method The following are needed: a side arm filter flask, a Pyrex microanalysis filter holder,* gridded