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Optimized inoculation procedures are an important consideration in achieving repeatable plant infection when working with biotrophic rust fungi. Several plant pathology laboratories specializing in rust research employ a system where the collection and application of fungal spores are accomplished using an exchangeable gelatin capsule. Urediniospores are collected from erumpent pustules on plant surfaces into a capsule fitted to a cyclone collector controlled by a vacuum pump. By adding light mineral oil to the same capsule, the spore suspension is then sprayed onto plants by means of a dedicated atomizer (inoculator) connected to an air pressure source. Although devices are not commercially available, modern day technologies provide an opportunity to efficiently design and manufacture collectors and inoculators. Using a process called Additive Manufacturing (AM), also known as “3D printing,” the bodies of a collector and inoculator were digitally designed and then laser-sintered in nylon. Depending on availability, copper or aluminum tubes were fitted to the bodies of both devices afterward to either facilitate directed collection of spores from rust pustules on plant surfaces or act as a siphon tube to deliver the spore suspension contained in the capsule. No statistical differences were found between AM and metal inoculators for spray delivery time or spore deposition per unit area. In replicated collection and inoculation tests of wheat seedlings with urediniospore bulks or single pustule collections of Puccinia triticina and P. graminis f. sp. tritici, the causal organisms of leaf rust and stem rust, consistent and satisfactory infection levels were achieved. Immersing used devices in acetone for 60 s followed by a 2 h heat treatment at 75°C produced no contaminant infection in follow-up tests.

Purpose - The purpose of the present work is to develop a methodology to manufacture patient-specific models (lead masks) to be used as protective shields during cancer treatment, using 3D photography, rapid prototyping (RP) and metal spraying. It is also intended to reduce the trauma experienced by the patient, by removing any physical contact as with conventional methods, and also to reduce the manufacturing lead time. Design/methodology/approach - Patient-specific data are collected using 3D photography. The data are converted to.STL files, and then prepared for building with an LS 380 in nylon polyamide. Next, the sculpted model is used as the mould in a newly patented metal-spraying device, spraying liquid metal on to the sculpted surface. Findings - Intricate body geometries can be reproduced to effectively create metal shields, to be used in radiography applications. The models created fit the patients more accurately than through conventional methods, reducing the trauma experienced by the patient, and in a reduced time-frame, at similar costs to conventional methods. The new process and its materials management are less of a an environmental risk than conventional methods. Research limitations/implications - Access to 3D photography apparatus will be necessary, as well as to RP or CNC equipment. Using this approach, files can be transferred to a central manufacturing facility, i.e. hospitals or treatment units do not need their own facilities. Added implications are the design of jigs and fixtures, which will ensure accuracy in reuse. Practical implications - Metal shields can be created with ease and great accuracy using RP machines. It takes less time without inflated costs. Models are more accurately and easy to use, with less trauma experienced by the patient during the manufacturing phase. Originality/value - Novel applications, combined with a new process. The research expands the fast-growing field of medical applications of RP technologies. Its practical application will benefit patients on a daily basis. [ABSTRACT FROM AUTHOR]

Functional design is closely linked to manufacturing and building. Designers' freedom to express themselves is often limited by the capabilities of craftsmen who have to give physical substance to the designer's ideas. This paper reviews the use of rapid prototyping (RP) to construct complex geometry. Three-dimensional computer aided design data are transferred to a build volume on a 2D layer-by-layer basis. This manufacturing method results in the rapid production of a physical model that can be used to verify designs, check form, fit and functionality, as well as to create a depth perspective. The paper describes a fresh approach into an old industry, i.e. model making. Results proved that models built by conventional methods can be cost-effectively substituted by RP methods without the surface limitations created by cardboard models. [ABSTRACT FROM AUTHOR]