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ABSTRACT Sunflower cake is an alternative feed with nutritional potential to be used in poultry feeding. However, the high fiber content and the presence of antinutritional factors can compromise the availability of nutrients in the diet and influence the bone development of pullets, which can be mitigated with the dietary inclusion of exogenous enzymes. In this context, the objective of this study was to evaluate the effect of multi-enzyme supplementation in rations containing sunflower cake to pullets on mineral digestibility and quality and bone mineral composition. A total of 1020 pullets 7-weeks-age from the commercial strain Hy-Line, 540 white pullets and 480 brown pullets, were distributed in a randomized experimental design in a 2×2+1 factorial arrangement, with two sunflower cake levels (10 and 20% for white pullets and 12 and 24 % for brown pullets); two ways of enzymatic supplementation (absence and presence of multi-enzyme complex), and one control diet based on corn and soybean meal, totaling five treatments with six replicates of 18 white pullets and 16 brown pullets. Between week 14 and week 15, a metabolic trial was performed to determine the digestibility coefficient of ration minerals. To assess bone quality of the tibia and femur, one pullet of the 12th and 17th week of age was selected and killed. There was no significant interaction between the tested factors (sunflower cake x multi-enzyme complexes) for any of the variables studied. The inclusion level of sunflower cake did not influence the growth and bone quality parameters of the pullets during the growing phase. However, a presence of the multi-enzyme complex provided significant improvements in Ca, P and Mg digestibility coefficient. It is concluded that the multi-enzyme supplementation in the diets increases the digestibility of the mineral in the rations, contributing to the maintenance of bone quality of pullets fed with up to 24% sunflower cake.

Abstract The objective of this work was to evaluate the effect of the dietary addition of calcium anacardate as a source of phenolic compounds on the reproductive performance and blood antioxidant parameters of New Zealand White female rabbits. Twenty-seven New Zealand White female rabbits were distributed in a randomized complete block design with three treatments and nine replicates, considering one animal as the experimental unit. The treatments consisted of pelleted diets with different inclusion levels of calcium anacardate (0, 0.5, and 1.0%). The inclusion of calcium anacardate in the diets increased the concentration of phenolic compounds and the antioxidant capacity in the blood of lactating female rabbits. Calcium anacardate at the level of 1.0% improves the feed conversion ratio during gestation and increases the phenolic compounds and antioxidant capacity in the blood serum of female rabbits at 21 days of lactation.

Abstract Background The epithelial-mesenchymal transition (EMT) promotes cell signaling and morphology alterations, contributing to cancer progression. Exosomes, extracellular vesicles containing proteins involved in cell-cell communication, have emerged as a potential source of biomarkers for several diseases. Methods Our aim was to assess the proteome content of exosomes secreted after EMT-induction to identify potential biomarkers for ovarian cancer classification. EMT was induced in the ovarian cancer cell line CAOV3 by treating it with EGF (10 ng/mL) for 96 h following 24 h of serum deprivation. Subsequently, exosomes were isolated from the supernatant using selective centrifugation after decellularization, and their characteristics were determined. The proteins present in the exosomes were extracted, identified, and quantified using Label-Free-Quantification (LFQ) via Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). To identify potential biomarkers, the obtained proteomic data was integrated with the TGGA database for mRNA expression using principal component analysis and a conditional inference tree. Results The exosomes derived from CAOV3 cells exhibited similar diameter and morphology, measuring approximately 150 nm, regardless of whether they were subjected to EMT stimulation or not. The proteomic analysis of proteins from CAOV3-derived exosomes revealed significant differential regulation of 157 proteins, with 100 showing upregulation and 57 downregulation upon EMT induction. Further comparison of the upregulated proteins with the TCGA transcriptomic data identified PLAU, LAMB1, COL6A1, and TGFB1 as potential biomarkers of the mesenchymal HGSOC subtype. Conclusions The induction of EMT, the isolation of exosomes, and the subsequent proteomic analysis highlight potential biomarkers for an aggressive ovarian cancer subtype. Further investigation into the role of these proteins is warranted to enhance our understanding of ovarian cancer outcomes.

A intensificação do avanço do neoextrativismo e dos megaprojetos tem gerado um amplo processo de Estado de Exceção contínuo, sobretudo, para os povos originários. Um dos dispositivos utilizados para alavancar esses projetos é a dimensão institucional e as normativas promovidas pelo corporativismo da elite política do congresso nacional. Este artigo analisa as práticas necropolíticas do Estado brasileiro no contexto de pandemia da Covid-19, refletindo por um lado como o Estado priorizou as projetos ecocidas e genocidas vinculados ao neoextrativismo e banalizou a crise de saúde, e, por outro lado, analisamos a importância da resistência ancestral indígena em defesa da vida. Para isso, utilizamos a revisão bibliográfica narrativa, a análise de documentos-chaves dos movimentos indígenas e entrevistas construídas entre 2020 e 2022. Os caminhos pavimentados pelos povos, evidenciam importantes semeaduras e alteranativas para defendermos a sociobiodiversidade e o território como eixos imprescindiveis para enfrentarmos a crise sistêmica propagada pelas fronteiras do capital

O objetivo do estudo foi avaliar o efeito da inclusão do feno da folha de leucena em dietas sobre a metabolização de nutrientes, desempenho e características dos ovos de poedeiras comerciais. Foram utilizadas 216 poedeiras com 32 semanas de idade, distribuídas em delineamento inteiramente casualizado com 6 tratamentos, 6 repetições de 6 aves cada, durante 12 semanas. Os tratamentos consistiram em uma dieta formulada com milho, uma dieta formulada com sorgo e outras 4 formuladas com sorgo contendo 2, 4, 6 e 8% de feno da folha de leucena. Foi observada redução linear sobre os coeficientes de metabolizabilidade da matéria seca, proteína bruta, energia bruta e nos valores de energia metabolizável aparente e energia metabolizável aparente corrigida para balanço de nitrogênio. Observou-se redução linear na produção de ovos e na ingestão de energia metabolizável aparente corrigida para balanço de nitrogênio com o aumento da leucena nas dietas. Galinhas alimentadas com dietas contendo 8% de leucena apresentaram menor produção de ovos e ingestão de energia metabolizável aparente corrigida para balanço de nitrogênio em relação as aves alimentadas com o sorgo. A inclusão de 2% de feno da folha da leucena na dieta proporcionou gemas com pigmentação superior às obtidas com as dietas a base de milho. De acordo com os resultados, pode-se incluir até 6% de feno da folha de leucena em dietas de poedeiras comerciais formuladas com sorgo.

ABSTRACT The use of non-conventional feeds in poultry farming can reduce production costs, mainly because of its low cost, availability and chemical composition. Thus, parboiled whole rice bran (PWRB) appears as a potential use in feeding quails. Two experiments were carried out to evaluate the effects of the inclusion of PWRB in the diet of quails. In each experiment, a total of 288 seven-day-old European quails of both sexes, were distributed in a completely randomized design with six treatments and six replicates of eight quails each. The treatments consisted in the inclusion of PWRB in the proportions of 0; 5; 10; 15; 20 and 25% (first) and 0; 8; 16; 24; 32 and 40% (second). In the first experiment, a linear reduction in feed intake and weight gain was observed with the inclusion of PWRB above 5%, with no significant effect on feed conversion and carcass characteristics. However, there was no loss in the performance and carcass characteristics of quails fed with different levels of PWRB in comparison to the control group. In the second, it was observed that the inclusion of PWRB above 8% promoted a linear reduction in feed intake, weight gain and improvement in feed conversion, with no significant effect on carcass characteristics. However, quails fed 40% PWRB showed reduced intake and weight gain when compared to the control group. The use of PWRB provided an improvement in economic viability. Considering the effect on growth, PWRB can be used in the feeding of meat quails up to 32%.

O trabalho é uma análise bibliográfica sobre a perspectiva da ética da alteridade do Currículo de Pernambuco do Ensino Médio. Apresenta-se como objetivo abordar a perspectiva curricular de Pernambuco sobre a ética da alteridade para o ensino de filosofia em turmas do ensino médio da rede pública. A metodologia inicia com uma análise introdutória sobre a aprovação do novo ensino médio de Pernambuco a partir do Conselho Estadual de Educação (2021); o conceito de alteridade e circunstâncias e paradigmas que se referem a essa presente nas reflexões e filosofia de Emanoel Lévinas (1997); o Currículo de Pernambuco do Ensino Médio com as competências entrelaçadas com as Diretrizes Curriculares Nacionais para o Ensino Médio e as competências para as Ciências Humanas, além das proposições para o ensino filosofia no que tange os conteúdos, as expectativas e habilidades de aprendizagens; entre outros autores como Miranda (2008) que faz uma relação entre a alteridade e a educação e Zanon (2020) com a ética da alteridade para uma formação responsável.

A pandemia de covid-19 representou um grande desafio para a diferenciação dos sistemas sociais. O Brasil foi a segunda nação do mundo em número de vítimas, e a influência das decisões tomadas dentro de diferentes esferas sociais (em particular saúde, ciência, direito e mídia de massa) tenderam, no país, a afetar as demais de maneria imprópria. O propósito do presente estudo foi apresentar, com base nas teorias dos sistemas sociais de Luhmann, os perigos dessa desdiferenciação para a sociedade e, também, para o indivíduo. A metodologia consistiu na pesquisa bibliográfica. O estudo concluiu que a preservação da função de cada um dos sistemas citados é essencial para a preservação de suas respectivas autonomias e da saúde coletiva e individual em solo brasileiro.

Herbicides with glyphosate (GLY) as an active ingredient (a.i.) are increasingly used, and GLY is currently the most used herbicide in the world. Consequently, its residues have often been found in aquatic ecosystems. Investigating how this substance affects aquatic species is a priority in ecotoxicology research, especially in fish, as they can absorb and concentrate toxins. In this sense, a critical review was performed, synthesizing data from the peer-reviewed bibliography, reporting on the toxicity of exposure to pure GLY and glyphosate-based herbicides (GBHs), using zebrafish as an animal model. The concentrations of this herbicide that induced toxic effects are highly variable, with some exceeding the limits determined by regulatory agencies. Globally, relevant toxic effects have been reported in zebrafish, namely, teratogenic effects incompatible with life, which translates directly into an increase in reported zebrafish mortality. Neurotoxicity, genotoxicity, changes in energy metabolism and oxidative stress, and immune and hormonal system dysfunction with an impact on fish reproduction were also described. In conclusion, both GLY and GBHs may induce damage to zebrafish, compromising their survival, reproduction, and maintenance. These results may be valid and applied to other fish species and aquatic ecosystems.

Thymol (THY) and 24-epibrassinolide (24-EPI) are two examples of plant-based products with promising therapeutic effects. In this study, we investigated the anti-inflammatory, antioxidant and anti-apoptotic effects of the THY and 24-EPI. We used zebrafish (Danio rerio) larvae transgenic line (Tg(mpxGFP)i114) to evaluate the recruitment of neutrophils as an inflammatory marker to the site of injury after tail fin amputation. In another experiment, wild-type AB larvae were exposed to a well known pro-inflammatory substance, copper (CuSO4), and then exposed for 4 h to THY, 24-EPI or diclofenac (DIC), a known anti-inflammatory drug. In this model, the antioxidant (levels of reactive oxygen species—ROS) and anti-apoptotic (cell death) effects were evaluated in vivo, as well as biochemical parameters such as the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), the biotransformation activity of glutathione-S-transferase, the levels of glutathione reduced and oxidated, lipid peroxidation, acetylcholinesterase activity, lactate dehydrogenase activity, and levels of nitric acid (NO). Both compounds decreased the recruitment of neutrophils in Tg(mpxGFP)i114, as well as showed in vivo antioxidant effects by reducing ROS production and anti-apoptotic effects in addition to a decrease in NO compared to CuSO4. The observed data substantiate the potential of the natural compounds THY and 24-EPI as anti-inflammatory and antioxidant agents in this species. These results support the need for further research to understand the molecular pathways involved, particularly their effect on NO.

Internationalization in higher education is essential, and although active learning methodologies are increasing and allow students to develop transversal skills, most still have a very local scope. In this context, the Collaborative Online International Learning (COIL) methodology is an interesting approach to benefit the students' development. It consists of an online program that involves creating multicultural teams to develop a specific learning project. Although this methodology is expanding, its use in physiology is still scarce. This paper aims to show an example of applying COIL methodology in physiology topics to enhance higher-education students' innovation and business skills. Our example project developed a sports-assessment service concept focused on physiology and biomechanics assessments. The program involved teams from Brazil, Germany, and Spain, comprising undergraduate and master students. Over 7 weeks, these teams, mentored by professors and researchers, engaged in workshops covering COIL methodology, business model design, executive summary planning, economic analyses, and communication techniques. Key outcomes included learning new concepts, developing soft skills, building confidence in innovative solution proposals, and experiencing diverse cultures. Challenges faced were language barriers, scheduling, task complexity, and logistical issues. This experience confirms the effectiveness of incorporating programs using COIL methodology into educational curriculums. Doing so exposes physiology students to innovation, entrepreneurship, and business creation while strengthening their professional connections and opening up postgraduation opportunities.

O presente artigo foi realizado em função do uso frequente da hashtag “Bandido bom é Bandido Morto” pelos usuários brasileiros de redes sociais Twitter e Facebook. O propósito do estudo foi o de verificar para quais tipos de crimes a hashtag é utilizada. Dessa maneira, o estudo foi conduzido por meio do método quantitativo, utilizando o foco interacional, coletando-se dados do Twitter e do Facebook de novembro de 2017 a dezembro de 2018. Os resultados demonstram que a utilização da hastag é conectada com os crimes comuns e com os crimes econômicos.

Abstract Non-T cell activation linker (NTAL) is a lipid raft-membrane protein expressed by normal and leukemic cells and involved in cell signaling. In acute promyelocytic leukemia (APL), NTAL depletion from lipid rafts decreases cell viability through regulation of the Akt/PI3K pathway. The role of NTAL in APL cell processes, and its association with clinical outcome, has not, however, been established. Here, we show that reduced levels of NTAL were associated with increased all-trans retinoic acid (ATRA)-induced differentiation, generation of reactive oxygen species, and mitochondrial dysfunction. Additionally, NTAL-knockdown (NTAL-KD) in APL cell lines led to activation of Ras, inhibition of Akt/mTOR pathways, and increased expression of autophagy markers, leading to an increased apoptosis rate following arsenic trioxide treatment. Furthermore, NTAL-KD in NB4 cells decreased the tumor burden in (NOD scid gamma) NSG mice, suggesting its implication in tumor growth. A retrospective analysis of NTAL expression in a cohort of patients treated with ATRA and anthracyclines, revealed that NTAL overexpression was associated with a high leukocyte count (P = 0.007) and was independently associated with shorter overall survival (Hazard Ratio: 3.6; 95% Confidence Interval: 1.17–11.28; P = 0.026). Taken together, our data highlights the importance of NTAL in APL cell survival and response to treatment.

This work is part of the research project developed by the Observatory on New Social Movements and Law in Brazil at La Salle University, focusing in the city of Porto Alegre and on the occupation of the City Council on July of 2013, which is placed in the context of the protests against the increase of bus fares and for free fare, attempting to understand the relationship between the political organization of the Bloco de Lutas pelo Transporte Publico and its legal group during the eight days of City Council occupation. We conducted semi-structured interviews with members of the occupation in order to clarify the dynamics in the movement and its understanding of the relationship between law and social movements, highlighting the deferment of the eviction order and the elaboration of two Bills as fundamental moments of the relationship between the collective organization of the occupation and its legal team. Este artículo forma parte de un proyecto de investigación desarrollado por el Observatorio de Nuevos Movimientos Sociales y Derecho de la Universidad La Salle de Brasil. Nos centramos en la ciudad de Porto Alegre y en la ocupación de su ayuntamiento en julio de 2013, en el contexto de las protestas contra el aumento de las tarifas de autobús y a favor del transporte gratuito. Intentamos comprender la relación entre la organización política del Bloco de Lutas pelo Transporte Publico y su grupo jurídico durante los ocho días que duró la ocupación. Realizamos entrevistas semiestructuradas con miembros de la ocupación para aclarar las dinámicas del movimiento y cómo entendía la relación entre derecho y movimientos sociales, destacando el aplazamiento de la orden de desalojo y la elaboración de dos leyes como momentos fundamentales de la relación entre la organización colectiva de la ocupación y su equipo jurídico. Available from: https://doi.org/10.35295/osls.iisl/0000-0000-0000-1040

Abstract Anacardic acid is naturally found in various parts of the cashew tree (Anacardium occidentale L.) and marketed as calcium anacardate. This product has antibacterial, antifungal and antioxidant activity, and has been used in humans in the treatment and prevention of cardiovascular and cerebrovascular diseases and tumors. In meat and egg production systems, anacardic acid is used as a substitute for growth-promoting antibiotics. The study objective was to evaluate effects of adding calcium anacardate as source of anacardic acid in laying Japanese quail diet on bird performance and eggs quality. A total of 252 Japanese quail with 22 week-old were studied, using a completely randomized design composed of six treatments with six replicates and seven quails per experimental unit. Treatments applied were: diet without growth promoter; diet with 0,1% growth promoter; and four diets without growth promoter and addition of 0.25; 0.50; 0.75 and 1.0% calcium anacardate (CA), respectively. The data were submitted to analysis of variance and treatment means compared with a SNK test (5%). The data from treatments containing CA were submitted to regression analysis. Treatments did not influence feed intake, egg production, egg weight, egg volume, feed conversion ratio and eggs quality parameters. The addition up to 1% of calcium anacardate in laying Japanese quail diet, does not influence their performance and egg quality.

Abstract Synthetic antioxidants (e.g.butylhydroxytoluene, BHT) are routinely used for to restrict oxidative processes of meat products, but they are implicated as harmful to the health of humans. Therefore natural alternatives, such as plant antioxidants, have been sought as replacements. Plant antioxidants when added to the diet can be incorporated into meat and reduce the need for the addition of synthetic antioxidants during processing. The objective of this study was to evaluate the effects of ethanol extracts of mango seeds (EEMS) in the diet of pigs on qualitative parameters and total antioxidant capacity of mortadella produced from these animals. Thirty-two pigs with an average 60 days of age were distributed among four treatments: control=no antioxidant; BHT=200ppm BHT; EEMS200=200ppm of EEMS and EEMS400=400ppm of EEMS. At 145 days of age the animals were slaughtered and loin was removed for the preparation of mortadella, which was analyzed during 90 days of storage at 4°C. A higher content of polyphenolic compounds and, total antioxidant capacity in mortadellas processed with meat of animals which consumed the EEMS400 ration after 60 and 90 days of storage was observed. EEMS polyphenolic antioxidants incorporated into pork through the diet results in an increase of total antioxidant capacity in the processed product.

Few studies on the development of forgiveness involved young children and adolescents, and very few involved samples from non-western countries. This study focused on the development of willingness to forgive a particular transgression in participants aged 4 to 12 years and from two different cultures: a South African culture (Mozambique) and a Western European culture (France). Overall, 153 pupils from Mozambique and 107 pupils from France were presented with eight vignettes depicting a situation where a young child, intentionally or accidentally, dropped a small radio that they borrowed from another young child. These vignettes were composed by applying an orthogonal Intent × Consequences × Apologies, 2 × 2 × 2, design. Participants expressed what their willingness to forgive would be in each case on a continuous response scale. Through cluster analysis, four main positions were identified. They were labeled: "Almost never forgive" (11% of participants), "Depends mainly on consequences" (22%), "Depends mainly on intent and apologies" (57%), and "Almost always forgive" (2%). The first two positions were typical of 4-7-year olds, and the third one was typical of 8-12-year olds. No significant differences in cluster composition were found between the Mozambican and French participants. Up to the age of four, most children probably do not have access to the notion of forgiveness. From the age of four to seven, this notion begins to appear, in particular through a gradual taking into account of circumstantial factors other than the severity of consequences of a transgression. This development is quite rapid - probably during the 7-year period, from 0 to 7 years. From the age of eight onwards, a forgiveness schema similar to that observed in adults is operational. This developmental trajectory is probably very general. It seems to depend little on the type of culture in which it develops.

Increasing awareness regarding fossil fuel dependence, waste valorization, and greenhouse gas emissions have prompted the emergence of new solutions for numerous markets over the last decades. The tire industry is no exception to this, with a global production of more than 1.5 billion tires per year raising environmental concerns about their end-of-life recycling or disposal. Pyrolysis enables the recovery of both energy and material from end-of-life tires, yielding valuable gas, liquid, and solid fractions. The latter, known as recovered carbon black (rCB), has been extensively researched in the last few years to ensure its quality for market applications. These studies have shown that rCB quality depends on the feedstock composition and pyrolysis conditions such as type of reactor, temperature range, heating rate, and residence time. Recent developments of activation and demineralization techniques target the production of rCB with specific chemical, physical, and morphological properties for singular applications. The automotive industry, which is the highest consumer of carbon black, has set specific targets to incorporate recycled materials (such as rCB) following the principles of sustainability and a circular economy. This review summarizes the pyrolysis of end-of-life tires for the production of syngas, oil, and rCB, focusing on the process conditions and product yield and composition. A further analysis of the characteristics of the solid material is performed, including their influence on the rCB application as a substitute of commercial CB in the tire industry. Purification and modification post-treatment processes for rCB upgrading are also inspected.

Este trabalho surge do retorno à teoria psicanalítica pelo método de diagnóstica não-toda. Ensejamos nos aproximar do que resiste à teorização, questionando os seus limites para regressar à prática clínica e produzir novas respostas acerca da noção do real na própria teoria da constituição do sujeito. Em termos metapsicológicos, podemos observar três dimensões de construção teórica sobre a constituição do sujeito: (1) as estruturas ontológicas como o Real, o Simbólico e o Imaginário em sua lógica de trançamento – aspecto topológico; (2) as estruturas antropológicas como metáfora paterna, falo e significante em sua perspectiva estrutural – aspecto dinâmico; e (3) a noção de objeto a e as posições discursivas que permitem a passagem entre as estruturas ontológicas e antropológicas – aspecto econômico. Apresentamos as construções dinâmicas e topológicas sobre a constituição do sujeito e questionamos a ausência de trabalhos acerca do aspecto econômico.

Abstract: The objective of this work was to evaluate the effect of the inclusion of vegetable and mixed glycerin in the diet of broiler quail (Coturnix coturnix coturnix) on animal performance, yield of carcass and organs, and physical and sensory characteristics of the meat. A total of 432 quails aged 1-42 days were used in a completely randomized design with a 4×2+1 factorial arrangement (5, 10, 15, and 20% inclusion of vegetable or mixed glycerin, besides one treatment without glycerin) with 4 replicates of 12 birds each. Feed intake, weight gain, feed conversion, yield (carcass, breast, thigh+drumstick, heart, liver, gizzard, and abdominal fat), and physical and sensory characteristics of the meat. The diet containing mixed glycerin resulted in higher feed intake and feed conversion. The yield of carcass, meat cuts, and organs, as well as the sensory characteristics of the meat, did not vary between the treatments. Abdominal fat content and shear strength were higher in birds fed diets containing vegetable glycerin. The tested glycerin and levels do not interfere with weight gain, yield, and sensory quality of the meat. However, weight gain and feed conversion are lower when mixed glycerin is used, although meat tenderness is higher when 15% vegetable glycerin is used.

INTRODUCTION: The study of the final elongation values produced by 45º, 60º, 75º, and 90º-angle z-plasties performed on the skin of the lower abdomen of fresh cadavers were compared with the theoretical mathematical data published in the literature. METHODS: Four z-plasties with 2-cm main and secondary branches each, and with 45º, 60º, 75º, and 90º angles were performed on the skin of the lower abdomen in 11 fresh cadavers to evaluate the final elongation produced. RESULTS: The mean elongation at their respective angles was lower than that found in the literature, with a p value of

The therapeutic potential of mesenchymal stem/stromal cells (MSC) is widely recognized for the treatment of several diseases, including acute graft-vs.-host disease (GVHD), hematological malignancies, cardiovascular, bone, and cartilage diseases. More recently, this therapeutic efficacy has been attributed to the bioactive molecules that these cells secrete (secretome), now being referred as medicinal signaling cells. This fact raises the opportunity of therapeutically using MSC-derived soluble factors rather than cells themselves, enabling their translation into the clinic. Indeed, many clinical trials are now studying the effects of MSC-secretome in the context of cell-free therapy. MSC secretome profile varies between donors, source, and culture conditions, making their therapeutic use very challenging. Therefore, identifying these soluble proteins and evaluating their production in a reproducible and scalable manner is even more relevant. In this work, we analyzed the global profile of proteins secreted by umbilical cord matrix (UCM) derived-MSC in static conditions by using mass spectrometry, enabling the identification of thousands of proteins. Afterwards, relevant proteins were chosen and monitored in the supernatant of a fully-controllable, closed and scalable system (bioreactor) by using multiple reaction monitoring (MRM) mass spectrometric technique in a time-dependent manner. The results showed that the majority of interesting proteins were enriched through time in culture, with the last day of culture being the ideal time for supernatant collection. The use of this regenerative “soup,” which is frequently discarded, could represent a step toward a safe, robust and reproducible cell-free product to be used in the medical therapeutic field. The future use of chemically defined culture-media will certainly facilitate secretome production according to Good Manufacturing Practice (GMP) standards.