Search

Although oxidative stress itself is directly associated with the occurrence of atherosclerosis, and together with endothelial dysfunction is a marker of atherosclerotic risk, none of these two markers were directly correlated with the presence of atherosclerosis. This study investigated the correlation between oxidative stress and endothelial dysfunction in people with cardiovascular disease. Furthermore, another objective of this paper is the direct study of the effect of a compound with antioxidant properties, selenium, on a model of endothelial dysfunction induced on human vascular fragments.

It can be observed that oxidative stress initiates and aggravates many diseases, including peptic ulcers and gastric carcinoma. An increase in rat mucosal glandular lipid peroxidation (LPO) and superoxide dismutase (SOD) and a decrease in catalase (CAT) levels in gastric ulceration induced by cold retention stress were observed, whereas in patients with ulcerated clinical peptic and gastric carcinoma LPO serum is associated with a decrease in SOD and CAT. It was intended to determine the parameters of oxidative stress in the blood and brain in rats after induction of gastric ulcer, the effect of antioxidant treatment on these parameters and on the appearance of gastric ulcer, determination of correlation between oxidative stress and severity of gastric ulcer in rats and also evaluation of vascular endothelial function by in vitro studies in the organ bath, from rat aortic rings with gastric ulcer vs. normal and others under treatment vs. untreated.

Arterial endothelium produces a large ramge of active factors which are indispensable for modulation of vasomotor tone and maintenance of vascular wall integrity. From these factors, nitric oxide (NO), wich is released by the endothelial cells as a response to acetylcholine or adenosine action on specific receptors, plays an important role.NO is the result of oxidation process of L-arginine into L-citrulline, under the action of endothelial nitric oxide synthase (NOSe), wich is activated by intracelluar Ca2+ - calmodulin complex . Our study, performed in isolated organ bath, analyzed vascular reactivity of 12 guinea pigs� thoracic aorta rings. After phenylephrine -PHE 10-5 mol/L precontraction, the dose-effect curves for acetylcoline � ACH, adenosine 5� phosphate - 5�ADP and sodium nitroprusside � SNP were determined, before and after incubation of preparation, for 1 hour, with 5% hydrosoluble cigarettes smoke extract (CSE). Statistic analysis, performed with the use of t pair test and ANOVA parametric test, showed that incubation of vascular preparation with 5% CSE has increased the contractile response to PHE 10-5 mol/L (p[0.05), has reduced the endothelium-dependent relaxing response to ATP 10-5 mol/L (p[0.001) and 5�ADP 10-5 molo/L (p[0.001), but has not significantly modified the endothelium-independent relaxing response to SNP 10-5 mol/L (p=0.05). As a conclusion, vascular rings incubation with 5% CSE induced a decrease of endothelium NO synthesis under the action of AXH and 5�ADP, but did not change the smooth muscle fiber respomse in the presence of NO released by SNP.

A longitudinal, randomized, five year follow-up clinical and experimental study was conducted during May 1, 2011 - June 30, 2016. A number of 125 eligible postmenopausal women (BMD T-score spine (-2.8 � 0.5)); BMD at the femoral hip (-2.6 � 0.8), treated only with biphosphonates, were enrolled in the study. The patient�s age ranged between 60 and 65 years. All the women were stone formers, with more than five episodes of crystalluria in the last five years. Exclusion criteria: kidney chronic diseases, use of drugs known to affect bone metabolism and renal function, patients with multi-morbidities. The aim of our study was to assess the connection between postmenopausal osteoporosis and the risk of worsening calcium balance in urolithiasis, based on the complex thermogravimetric findings and porosity studies of renal stones eliminated by these patients. The study of renal-urinary concretions offers the possibility to amplify the specific effects of the thermal decomposition processes of substances, reflecting the sample�s global composition. The research of calculi porosity gives information about the dissolution possibilities of the renal-urinary concretions. In conclusion, in postmenopausal women, proper administration of bisphosphonate therapy must accompany the supplementary medication with calcium and vitamin D, always correlated to the metabolic status of the osteoporotic patient.

High blood pressure (HPB) is considered a major health problem affecting more than one billion people worldwide. Hypertension is the most common cardiovascular disorder that increases the risk of cardiovascular morbidity and mortality. HDL-cholesterol, considered to be an independent risk factor for cardiovascular events, presents pleiotropic effects that can influence arterial status and blood pressure. The aim of this study was to determine the correlation between HDL-cholesterol levels and blood pressure in normotensive and hypertensive patients (untreated and 1-month treated with statins) and to evaluate the arterial stiffness as a marker of endothelial dysfunction in these patients.

In this paper, it was analysed the influence of formulation factors over obtaining oxicam hydrogels, using the statistical analysis. Data analysis and predictive modeling by multivariate regression offers a large number of possible explanatory/predictive variables. Therefore, variable selection and dimension reduction is a major task for multivariate statistical analysis, especially for multivariate regressions. The statistical analysis and computational data processing of responses obtained from different pharmaceutical formulations, via different experimental protocols, lead to the optimization of the formulation process. It was found that the most suitable pharmaceutical formulations based on oxicams with the possibility of rapid release contained cyclodextrin, in particular 2-hydroxypropyl-b-cyclodextrin.

The present available data on the harmful effects of electronic cigarettes (EC) is inconsistent and contradicting. Even though EC is considered a healthier alternative to the traditional cigarettes, several studies reported a large range of toxic compounds. The objective of this study is to determine whether the mercury found in the solutions used for electronic cigarettes is involved in the initiation and aggravation of endothelial dysfunction found in smoking persons with coronary artery disease (CAD). Endothelial function was evaluated by performing organ bath studies of vascular rings from patients with CAD, and was determined as a response to cumulative doses of endothelial-dependent/independent vasodilators, in the presence of Hg2+. Hg2+ extraction was realised using ion exchanging resin PUROLITE S920 from the liquid solution used in EC. The results of this study suggests that Hg2+ may be responsible for the aggravation of the endothelial dysfunction found in smoker of EC patients with CAD.

The experimental investigations aimed to determine the optimal conditions for the process of zinc removal from thermal waters with the PUROLITE S920 ion exchange resin. It was determined the residual zinc concentration dependence on the amount of ion exchanger at different initial zinc concentrations in solution and different stirring times. The optimum conditions for achieving a residual concentration below 1 mg/L are: ion exchange rate 1.6 g/L, stirring time about 45 min. The PUROLITE S920 ion exchanger can be successfully used to remove very small amounts of zinc ions from the solution.

On a total of 150 renal corrosion casts were examined the posterior branch and the artery of renal posterior segment. For the origin of the posterior branch, there have been highlighted three morphological types, namely: Type I, bifurcation in anterior and posterior branches (92.67% of cases); Type II, trifurcation in anterior, posterior and superior or inferior branches (6.00% of cases); Type III, quadrifurcation in anterior, posterior, superior and inferior branches (1.33% of cases). Depending on the relations between this path and those with the posterior aspect of the renal pelvis, we highlighted four morphological types: Type I (62.67% of cases) with large extension of the posterior segmental artery, convex downward path and multiple subsegmentary branches; Type II (34.00% of cases) with large extension of the posterior segmental artery, that bifurcates or trifurcates in segmental branches and convex downward path; Type III (2.00% of cases) with small extension of the posterior segmental artery, with downward path in relation to the upper portion of the posterior surface of the renal pelvis upper portion; Type IV (1.33% of the cases) with small extension of the posterior segmental artery, with upward trajectory in relation to the lower portion of the renal pelvis. Knowledge of these aspects is important both to investigate morphological imaging and for performing partial resection of the renal parenchyma.

In this paper it has been done an experimental work regarding the optimal conditions of removing mercury from water using ion exchange resins and cellulose xanthate (CX/Na). It were prepared water solution of HgCl2 with very well determined concentrations where it was added different amount of ion exchanger resin and CX/Na. The system was shaking 20, 40, 60 and 80 min. It has been found that the optimal conditions for removing of mercury from contaminated water solutions are 2.5 g/L CX/Na for 60 min shaking time for 100mg/L initial mercury concentration and 2.0 g/L CX/Na for 60 min shaking time for 50mg/L initial mercury concentration.

Betulinic acid, a very promising anti-melanoma agent, has very low water solubility that causes low bioavailability. To overcome this inconvenience, a highly water-soluble cyclodextrin was used (octakis-[6-deoxy-6-(2-sulfanyl ethanesulfonic acid)]-γ-cyclodextrin). The complex was physico-chemically analyzed using differential scanning calorimetry (DSC), X-ray and scanning electron microscopy (SEM) methods and then in vitro tested for its antiproliferative activity by the MTT assay and by cell cycle analysis. Finally, the complex was tested in vivo using an animal model of murine melanoma developed in C57BL/6J mice, where it caused a reduction in tumor volume and weight. The study revealed the beneficial influence of betulinic acid inclusion into the cyclodextrin in terms of antiproliferative activity and in vivo tumor development.