1. The growth of cultured human foreskin keratinocytes is not stimulated by a tumor promoter
- Author
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Susan M. Fischer, Edith W. Wong, Charles E. Weeks, Thomas J. Slaga, Aurora Viaje, and Gerald D. Mills
- Subjects
Male ,Cancer Research ,Ornithine Decarboxylase ,Ornithine decarboxylase ,chemistry.chemical_compound ,Foreskin ,Epidermal growth factor ,medicine ,Humans ,Cells, Cultured ,Skin ,integumentary system ,Epidermis (botany) ,Epidermal Growth Factor ,General Medicine ,Molecular biology ,Phorbols ,medicine.anatomical_structure ,chemistry ,Tetradecanoylphorbol Acetate ,Immunology ,Polyamine ,Keratinocyte ,Cell Division ,Explant culture - Abstract
The tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) does not stimulate the growth of human epidermal cells in foreskin explant cultures; a dose-dependent inhibition is seen at doses higher than 10(-5) micrograms/ml. TPA also inhibits epidermal growth factor-stimulated growth and does not induce ornithine decarboxylase activity or increase polyamine levels. This is not due to the rapid breakdown of TPA, since TPA is not metabolized to any appreciable extent.
- Published
- 1984