1. Tissue-Specific Oxidative Stress Modulation by Exercise: A Comparison between MICT and HIIT in an Obese Rat Model
- Author
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Elise Madeuf, Geoffroy Delcros, Emilie Vazeille, Vincent Pialoux, Nicolas Barnich, Pierre Sauvanet, Florie Maillard, Allison Teixeira, Pascal Sirvent, Nathalie Boisseau, Monique Etienne, Carole Groussard, Antoine Sourdrille, Yolanda F. Otero, Lydie Combaret, Laboratoire Mouvement Sport Santé (M2S), École normale supérieure - Cachan (ENS Cachan)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques - Clermont Auvergne (AME2P), Faculté des Sciences du SportFaculté des Sciences du Sport, UFR STAPS-Université Clermont Auvergne (UCA), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Université Clermont Auvergne (UCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), USC-2018, Institut National de la Recherche Agronomique, Université de Rennes (UR)-École normale supérieure - Rennes (ENS Rennes)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre de Recherche en Nutrition Humaine Auvergne [CHU Clermont-Ferrand] (CRNH A), Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), Region AuvergneRhone-Alpes (PREVAMIC project), I-SITE project of the University of Clermont Auvergne (CAP 2025), INSERM (U1071), INRA (USC-2018), Association F. Aupetit (AFA), ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016), Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH Auvergne), and Pialoux, V.
- Subjects
0301 basic medicine ,Male ,Aging ,MESH: Rats, Zucker ,Adipose tissue ,MESH: Physical Conditioning, Animal ,MESH: Muscle, Skeletal / metabolism ,High-Intensity Interval Training ,medicine.disease_cause ,Biochemistry ,Interval training ,Antioxidants ,chemistry.chemical_compound ,0302 clinical medicine ,Malondialdehyde ,Medicine ,ComputingMilieux_MISCELLANEOUS ,chemistry.chemical_classification ,MESH: Oxidative Stress ,biology ,lcsh:Cytology ,Glutathione peroxidase ,General Medicine ,Oxidants ,Lipoproteins, LDL ,Adipose Tissue ,Alimentation et Nutrition ,High-intensity interval training ,Research Article ,medicine.medical_specialty ,MESH: Rats ,Article Subject ,Superoxide dismutase ,03 medical and health sciences ,Internal medicine ,Physical Conditioning, Animal ,Food and Nutrition ,Animals ,Obesity ,lcsh:QH573-671 ,Xanthine oxidase ,Muscle, Skeletal ,Glutathione Peroxidase ,business.industry ,Superoxide Dismutase ,MESH: Adipose Tissue / metabolism ,NADPH Oxidases ,030229 sport sciences ,Cell Biology ,Rats ,Rats, Zucker ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,chemistry ,biology.protein ,business ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Oxidative stress ,Lipoprotein - Abstract
Background and Aim. Exercise is an effective strategy to reduce obesity-induced oxidative stress. The purpose of this study was to compare the effects of two training modalities (moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT)) on the pro/antioxidant status of different tissues in obese Zucker rats. Methods. Eight-week-old male Zucker rats (fa/fa, n=36) were subdivided in three groups: MICT, HIIT, and control (no exercise) groups. Trained animals ran on a treadmill (0° slope), 5 days/week for 10 weeks (MICT: 51 min at 12 m·min-1; HIIT: 6 sets of 3 min at 10 m·min-1 followed by 4 min at 18 m·min-1). Epididymal (visceral) and subcutaneous adipose tissue, gastrocnemius muscle, and plasma samples were collected to measure oxidative stress markers (advanced oxidation protein products (AOPP), oxidized low-density lipoprotein (oxLDL)), antioxidant system markers (ferric-reducing ability of plasma (FRAP), superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities), and prooxidant enzymes (NADPH oxidase and xanthine oxidase (XO) activities, myeloperoxidase content). Results. Compared with the control, MICT increased GPx and catalase activities and the FRAP level in epididymal adipose tissue. HIIT increased the AOPP level in subcutaneous adipose tissue. In the muscle, HIIT increased both SOD and GPx activities and reduced the AOPP level, whereas MICT increased only SOD activity. Finally, plasma myeloperoxidase content was similarly decreased by both training modalities, whereas oxLDL was reduced only in the MICT group. Conclusion. Both HIIT and MICT improved the pro/antioxidant status. However, HIIT was more efficient than MICT in the skeletal muscle, whereas MICT was more efficient in epididymal adipose tissue. This suggests that oxidative stress responses to HIIT and MICT are tissue-specific. This could result in ROS generation via different pathways in these tissues. From a practical point of view, the two training modalities should be combined to obtain a global response in people with obesity.
- Published
- 2019