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This study surveyed the indoor environment in Danish classrooms and explored its associations with pupil well-being and performance. It was a large-scale citizen science study using a simple intervention carried out over two days. On one of these days, the participating classes carefully aired out the classroom during the break before a lesson dedicated to measuring the classroom environment. They did this by keeping windows open and leaving the classroom during the break. On the other day, they were told to do as they usually do, i.e. they received no instruction to follow a particular airing behaviour. The order of the two airing behaviours was randomly balanced between classes. Measurements were reported by 709 classes in 234 schools and 640 classes completed a building checklist. In total, 21,326 well-being surveys and 20,701 concentration tests were completed by the pupils. Of these, a gross subsample of 13,094 records qualified for further analysis. With the instructed airing behaviour, the percentage of classes with a CO2 concentration higher than 1000 ppm was reduced from 53% to 36% as compared with uninstructed behaviour. This finding corresponded with earlier related studies carried out in Danish classrooms in 2014 and 2009. Airing also improved the pupils' perception of the classroom environment, alleviated their building-related symptoms and increased their performance of a concentration test in which they made 6% fewer errors than with uninstructed behaviour. Based on responses from a large number of pupils, the findings confirm that inadequate classroom ventilation negatively affects pupil well-being and concentration and that classroom air quality continues to present a challenge in many Danish school buildings.

A simplified modelling framework for the prediction of the indoor environment, energy use and socio-economic consequences of improving air quality and temperature in school buildings is suggested. The framework combines established models for infiltration and different modes of ventilation to estimate yearly distributions of the classroom temperature and CO2 concentration. These distributions are used as input to a prediction of pupil performance of schoolwork, their attendance at school, and teacher absence before and after refurbishment of their school building. Eventually, the framework estimates the socio-economic consequences of an improved classroom environment for a school and may be used to evaluate the feasibility of a range of different refurbishment scenarios and support decisions on building upgrades.

Organophosphates are used in a wide range of materials and consumer products and are ubiquitous in indoor environments. Certain organophosphates have been associated with various adverse health effects. The present paper reports mass fractions of organophosphates in dust samples collected from 500 bedrooms and 151 daycare centers of children living in Odense, Denmark. The identified compounds include: tris(isobutyl) phosphate (TIBP), tri-n-butyl phosphate (TNBP), tris(2-chloroethyl) phosphate (TCEP), tris(2-chloroisopropyl) phosphate (TCIPP), tris(1,3-dichloroisopropyl) phosphate (TDCIPP), tris(2-butoxyethyl) phosphate (TBOEP), triphenylphosphate (TPHP), 2-ethylhexyl-diphenyl phosphate (EHDPP), tris(2-ethylhexyl) phosphate (TEHP) and tris(methylphenyl) phosphate (TMPP). Both the number of organophosphates with median values above the limit of detection and the median values were higher for samples from daycare centers than for samples from homes. Organophosphates with median mass fractions above the limit of detection were: TCEP from homes (6.9 μg g-1), and TCEP (16 μg g-1), TCIPP (5.6 μg g-1), TDCIPP (7.1 μg g-1), TBOEP (26 μg g-1), TPHP (2.0 μg g-1) and EHDPP (2.1 μg g-1) from daycare centers. When present, TBOEP was typically the most abundant of the identified OPs. The sum of the organophosphate dust mass fractions measured in this study was roughly in the mid-range of summed mass fractions reported for dust samples collected in other countries. On a global scale, the geographical distribution of organophosphates in indoor dust is quite variable, with higher concentrations in industrialized countries. This trend differs from that for phthalate esters, whose geographic distribution is more homogeneous. Exposure to organophosphates via dust ingestion is relatively low, although there is considerable uncertainly in this assessment.

This study aims to elucidate in greater detail the dermal uptake of nicotine from air or from nicotine-exposed clothes, which was demonstrated recently in a preliminary study. Six non-smoking participants were exposed to gaseous nicotine (between 236 and 304 μg/m3 ) over 5 hours while breathing clean air through a hood. Four of the participants wore only shorts and 2 wore a set of clean clothes. One week later, 2 of the bare-skinned participants were again exposed in the chamber, but they showered immediately after exposure instead of the following morning. The 2 participants who wore clean clothes on week 1 were now exposed wearing a set of clothes that had been exposed to nicotine. All urine was collected for 84 hours after exposure and analyzed for nicotine and its metabolites, cotinine and 3OH-cotinine. All participants except those wearing fresh clothes excreted substantial amounts of biomarkers, comparable to levels expected from inhalation intake. Uptake for 1 participant wearing exposed clothes exceeded estimated intake via inhalation by >50%. Biomarker excretion continued during the entire urine collection period, indicating that nicotine accumulates in the skin and is released over several days. Absorbed nicotine was significantly lower after showering in 1 subject but not the other. Differences in the normalized uptakes and in the excretion patterns were observed among the participants. The observed cotinine half-lives suggest that non-smokers exposed to airborne nicotine may receive a substantial fraction through the dermal pathway. Washing skin and clothes exposed to nicotine may meaningfully decrease exposure.

Associations between learning, ventilation mode, and other classroom characteristics were investigated with data from a Danish test scheme and two widespread cross-sectional studies examining air quality in Danish schools. An academic achievement indicator as a measure of the learning outcome was calculated from the scores of a standardized Danish test scheme adjusted for a socioeconomic reference index. Pupils in schools with balanced mechanical ventilation had significantly higher achievement indicators than pupils in schools with natural ventilation, where airing took place mostly by manual window opening. Also, the carbon dioxide concentration was lower in classrooms with balanced mechanical ventilation. There was no consistent association between the achievement indicators and the person specific room volume, construction/renovation year, or the occupancy. Measurements of carbon dioxide concentrations and temperatures in 820 classrooms in 389 schools were available. In 56% and 66% of the classrooms included in the two studies, the measured CO2 concentration was higher than 1000 ppm. The findings of this study add to the growing evidence that insufficient classroom ventilation have impacts on learning outcomes.

To assess the influence of clothing on dermal uptake of semi-volatile organic compounds (SVOCs), we measured uptake of selected airborne phthalates for an individual wearing clean clothes or air-exposed clothes and compared these results with dermal uptake for bare-skinned individuals under otherwise identical experimental conditions. Using a breathing hood to isolate dermal from inhalation uptake, we measured urinary metabolites of diethylphthalate (DEP) and di-n-butylphthalate (DnBP) from an individual exposed to known concentrations of these compounds for 6 h in an experimental chamber. The individual wore either clean (fresh) cotton clothes or cotton clothes that had been exposed to the same chamber air concentrations for 9 days. For a 6-h exposure, the net amounts of DEP and DnBP absorbed when wearing fresh clothes were, respectively, 0.017 and 0.007 μg/kg/(μg/m3); for exposed clothes the results were 0.178 and 0.261 μg/kg/(μg/m3), respectively (values normalized by air concentration and body mass). When compared against the average results for bare-skinned participants, clean clothes were protective, whereas exposed clothes increased dermal uptake for DEP and DnBP by factors of 3.3 and 6.5, respectively. Even for non-occupational environments, wearing clothing that has adsorbed/absorbed indoor air pollutants can increase dermal uptake of SVOCs by substantial amounts relative to bare skin.

Exposure to ultrafine particles (UFP) may have adverse health effects. Central monitoring stations do not represent the personal exposure to UFP accurately. Few studies have previously focused on personal exposure to UFP. Sixty non-smoking residents living in Copenhagen, Denmark were asked to carry a backpack equipped with a portable monitor, continuously recording particle number concentrations (PN), in order to measure the real-time individual exposure over a period of similar to 48 h. A GPS logger was carried along with the particle monitor and allowed us to estimate the contribution of UFP exposure occurring in various microenvironments (residence, during active and passive transport, other indoor and outdoor environments) to the total daily exposure. On average, the fractional contribution of each microenvironment to the daily integrated personal exposure roughly corresponded to the fractions of the day the subjects spent in each microenvironment. The home environment accounted for 50% of the daily personal exposure. Indoor environments other than home or vehicles contributed with similar to 40%. The highest median UFP concentration was obtained during passive transport (vehicles). However, being in transit or outdoors contributed 5% or less to the daily exposure. Additionally, the subjects recorded in a diary the periods when they were at home. With this approach, 66% of the total daily exposure was attributable to the home environment. The subjects spent 28% more time at home according to the diary, compared to the GPS. These results may indicate limitations of using diaries, but also possible inaccuracy and miss-classification in the GPS data. (C) 2015 Elsevier Ltd. All rights reserved. (Less)

Background Fundamental considerations indicate that, for certain phthalate esters, dermal absorption from air is an uptake pathway that is comparable to or greater than inhalation. Yet this pathway has not been experimentally evaluated and has been largely overlooked when assessing uptake of phthalate esters. Objectives This study investigated transdermal uptake, directly from air, of diethyl phthalate (DEP) and di(n-butyl) phthalate (DnBP) in humans. Methods In a series of experiments, six human participants were exposed for 6 hr in a chamber containing deliberately elevated air concentrations of DEP and DnBP. The participants either wore a hood and breathed air with phthalate concentrations substantially below those in the chamber or did not wear a hood and breathed chamber air. All urinations were collected from initiation of exposure until 54 hr later. Metabolites of DEP and DnBP were measured in these samples and extrapolated to parent phthalate intakes, corrected for background and hood air exposures. Results For DEP, the median dermal uptake directly from air was 4.0 μg/(μg/m3 in air) compared with an inhalation intake of 3.8 μg/(μg/m3 in air). For DnBP, the median dermal uptake from air was 3.1 μg/(μg/m3 in air) compared with an inhalation intake of 3.9 μg/(μg/m3 in air). Conclusions This study shows that dermal uptake directly from air can be a meaningful exposure pathway for DEP and DnBP. For other semivolatile organic compounds (SVOCs) whose molecular weight and lipid/air partition coefficient are in the appropriate range, direct absorption from air is also anticipated to be significant. Citation Weschler CJ, Bekö G, Koch HM, Salthammer T, Schripp T, Toftum J, Clausen G. 2015. Transdermal uptake of diethyl phthalate and di(n-butyl) phthalate directly from air: experimental verification. Environ Health Perspect 123:928–934; http://dx.doi.org/10.1289/ehp.1409151

This cross-sectional study investigated the relationship between exposure to airborne indoor and outdoor particulate matter (PM) and cardiovascular and respiratory health in a population-based sample of 58 residences in Copenhagen, Denmark. Over a 2-day period indoor particle number concentrations (PNC, 10–300 nm) and PM2.5 (aerodynamic diameter

The climate in Greenland is cold which means that living inside the heated space requires quite some energy. To avoid large heat losses and cold discomfort, building envelopes are often sealed, which reduces natural infiltration. The combination of reduced infiltration and lack of mechanical ventilation results in low air change and thus elevated concentrations of indoor pollutants. In cold Arctic regions where people spend most of their time during long winters indoors is the effect of poor indoor air quality (IAQ) on occupants' health and comfort considerable. A cross sectional study in 79 dwellings was performed in the town of Sisimiut. The aim was to investigate the indoor climate in Greenlandic dwellings. Temperature, relative humidity (RH) and CO2 concentration were measured in several rooms in each dwelling. This paper presents the results from measurements in bedrooms. CO2 concentrations above 1000 ppm and difference in absolute humidity between indoor and outdoor air above 2.5 g/kg as indicators of insufficient ventilation were found in 73% of the bedrooms. The situation was significantly worse dwellings build after 1990. Although the average winter additional moisture was higher than 2.5 g/kg, the RH was low (mean RH = 26%). In summer, 19% of all bedroom temperatures were above 26 °C despite the low outside temperatures. To avoid possible escalation of health problems related to IAQ in the future and to increase comfort of the occupants, properly designed ventilation systems should be introduced in Greenland.

Six males clad only in shorts were exposed to high levels of airborne di(n-butyl) phthalate (DnBP) and diethyl phthalate (DEP) in chamber experiments conducted in 2014. In two 6 h sessions, the subjects were exposed only dermally while breathing clean air from a hood, and both dermally and via inhalation when exposed without a hood. Full urine samples were taken before, during, and for 48 h after leaving the chamber and measured for key DnBP and DEP metabolites. The data clearly demonstrated high levels of DnBP and DEP metabolite excretions while in the chamber and during the first 24 h once leaving the chamber under both conditions. The data for DnBP were used in a modeling exercise linking dose models for inhalation and transdermal permeation with a simple pharmacokinetic model that predicted timing and mass of metabolite excretions. These models were developed and calibrated independent of these experiments. Tests included modeling of the ""hood-on"" (transdermal penetration only), ""hood-off"" (both inhalation and transdermal) scenarios, and a derived ""inhalation-only"" scenario. Results showed that the linked model tended to duplicate the pattern of excretion with regard to timing of peaks, decline of concentrations over time, and the ratio of DnBP metabolites. However, the transdermal model tended to overpredict penetration of DnBP such that predictions of metabolite excretions were between 1.1 and 4.5 times higher than the cumulative excretion of DnBP metabolites over the 54 h of the simulation. A similar overprediction was not seen for the ""inhalation-only"" simulations. Possible explanations and model refinements for these overpredictions are discussed. In a demonstration of the linked model designed to characterize general population exposures to typical airborne indoor concentrations of DnBP in the United States, it was estimated that up to one-quarter of total exposures could be due to inhalation and dermal uptake.

Benzophenone-3 (also known as BP-3 or oxybenzone) is added to sunscreens, plastics, and some coatings to filter UV radiation. The suspected endocrine disruptor BP-3 has been detected in the air and settled dust of homes and is expected to redistribute from its original sources to other indoor compartments, including clothing. Given its physical and chemical properties, we hypothesized that dermal uptake from clothing could contribute to the body burden of this compound. First, cotton shirts were exposed to air at an elevated concentration of BP-3 for 32 days; the final air concentration was 4.4 μg/m3. Next, three participants wore the exposed shirts for 3 h. After 3 h of exposure, participants wore their usual clothing during the collection of urine samples for the next 48 h. Urine was analyzed for BP-3, a metabolite (BP-1), and six other UV filters. The rate of urinary excretion of the sum of BP-1 and BP-3 increased for all participants during and following the 3 h of exposure. The summed mass of BP-1 and BP-3 excreted during the first 24 h attributable to wearing exposed t-shirts were 12, 9.9, and 82μg for participants 1, 2, and 3, respectively. Analysis of these results, coupled with predictions of steady-state models, suggest that dermal uptake of BP-3 from clothing could meaningfully contribute to overall body burden.

Around the world humans use products that contain phthalates, and human exposure to certain of these phthalates has been associated with various adverse health effects. The aim of the present study has been to determine the concentrations of the metabolites of diethyl phthalate (DEP), di(n-butyl) phthalate (DnBP), di(iso-butyl) phthalate (DiBP), butyl benzyl phthalate (BBzP) and di(2-ethylhexyl) phthalate (DEHP) in urine samples from 441 Danish children (3-6 years old). These children were subjects in the Danish Indoor Environment and Children's Health study. As part of each child's medical examination, a sample from his or her first morning urination was collected. These samples were subsequently analyzed for metabolites of the targeted phthalates. The measured concentrations of each metabolite were approximately log-normally distributed, and the metabolite concentrations significantly correlated with one another. Additionally, the mass fractions of DEP, DnBP, DiBP and BBzP in dust collected from the children's bedrooms and daycare centers significantly correlated with the concentrations of these phthalates' metabolites (monoethyl phthalate (MEP), mono-n-butyl phthalate (MnBP), mono-isobutyl phthalate (MiBP) and monobenzyl phthalate (MBzP), respectively) in the children's urine. Such correlations indicate that indoor exposures meaningfully contributed to the Danish children's intake of DEP, DnBP, DiBP and BBzP. This was not the case for DEHP. The urine concentrations of the phthalate metabolites measured in the present study were remarkably similar to those measured in urine samples from children living in countries distributed over four continents. These similarities reflect the globalization of children's exposure to phthalate containing products.

The filamentous fungus Stachybotrys chartarum is known for its toxic metabolites and has been associated with serious health problems, including mycotoxicosis, among occupants of contaminated buildings. Here, we present results from a case study, where an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for known and tentatively identified compounds characterized via UHPLC-quadruple time-of-flight (QTOF) screening of fungal culture extracts, wall scrapings and reference standards. The UHPLC-MS/MS method was able to identify 12 Stachybotrys metabolites, of which four could be quantified based on authentic standards and a further six estimated based on similarity to authentic standards. Samples collected from walls contaminated by S. chartarum in a water-damaged building showed that the two known chemotypes, S and A, coexisted. More importantly, a link between mycotoxin concentrations found on contaminated surfaces and in settled dust was made. One dust sample, collected from a water-damaged room, contained 10 pg/cm2 macrocyclic trichothecenes (roridin E). For the first time, more than one spirocyclic drimane was detected in dust. Spirocyclic drimanes were detected in all 11 analysed dust samples and in total amounted to 600 pg/cm2 in the water-damaged room and 340 pg/cm2 in rooms adjacent to the water-damaged area. Their wide distribution in detectable amounts in dust suggested they could be good candidates for exposure biomarkers. Graphical abstract Stachybotrys growing on a gypsum board, and some of the compounds it produces Electronic supplementary material The online version of this article (doi:10.1007/s00216-016-9649-y) contains supplementary material, which is available to authorized users.

In this preliminary study, we have investigated whether dermal uptake of nicotine directly from air or indirectly from clothing can be a meaningful exposure pathway. Two participants wearing only shorts and a third participant wearing clean cotton clothes were exposed to environmental tobacco smoke (ETS), generated by mechanically "smoking" cigarettes, for three hours in a chamber while breathing clean air from head-enveloping hoods. The average nicotine concentration (420 mu g/m3) was comparable to the highest levels reported for smoking sections of pubs. Urine samples were collected immediately before exposure and 60 hour post-exposure for bare-skinned participants. For the clothed participant, post-exposure urine samples were collected for 24 hour. This participant then entered the chamber for another three-hour exposure wearing a hood and clothes, including a shirt that had been exposed for five days to elevated nicotine levels. The urine samples were analyzed for nicotine and two metabolites-cotinine and 3OH-cotinine. Peak urinary cotinine and 3OH-cotinine concentrations for the bare-skinned participants were comparable to levels measured among non-smokers in hospitality environments before smoking bans. The amount of dermally absorbed nicotine for each bare-skinned participant was conservatively estimated at 570 mu g, but may have been larger. For the participant wearing clean clothes, uptake was similar to 20 mu g, and while wearing a shirt previously exposed to nicotine, uptake was similar to 80 mu g. This study demonstrates meaningful dermal uptake of nicotine directly from air or from nicotine-exposed clothes. The findings are especially relevant for children in homes with smoking or vaping.

Exposure to particles in the fine and ultrafine size range has been linked to induction of low-grade systemic inflammation, oxidative stress and development of cardiovascular diseases. Declining levels of endothelial progenitor cells within systemic circulation have likewise been linked to progression of cardiovascular diseases. The objective was to determine if exposure to fine and ultrafine particles from indoor and outdoor sources, assessed by personal and residential indoor monitoring, is associated with altered levels of endothelial progenitor cells, and whether such effects are related to leukocyte-mediated oxidative stress. The study utilized a cross sectional design performed in 58 study participants from a larger cohort. Levels of circulating endothelial progenitor cells, defined as either late (CD34(+)KDR(+) cells) or early (CD34(+)CD133(+)KDR(+) cells) subsets were measured using polychromatic flow cytometry. We additionally measured production of reactive oxygen species in leukocyte subsets (lymphocytes, monocytes and granulocytes) by flow cytometry using intracellular 2',7'-dichlorofluoroscein. The measurements encompassed both basal levels of reactive oxygen species production and capacity for reactive oxygen species production for each leukocyte subset. We found that the late endothelial progenitor subset was negatively associated with levels of ultrafine particles measured within the participant residences and with reactive oxygen species production capacity in lymphocytes. Additionally, the early endothelial progenitor cell levels were positively associated with a personalised measure of ultrafine particle exposure and negatively associated with both basal and capacity for reactive oxygen species production in lymphocytes and granulocytes, respectively. Our results indicate that exposure to fine and ultrafine particles derived from indoor sources may have adverse effects on human vascular health.

Outdoor air delivery to buildings is an important parameter in the assessment of pollutant exposure indoors. Detailed and well controlled measurements of air exchange rates (AER) and interzonal airflows in residential environment are scarce. We measured the outdoor AERs in up to six rooms in five dwellings across four seasons using active tracer gas. Night time AERs were also estimated in the bedrooms based on occupant-generated CO2. Passive tracer gas measurements were performed for comparison. AERs changed frequently during the day. Differences in outdoor AERs were observed between individual rooms. Window opening behavior had a strong influence on AERs, which were highest during occupied daytime periods, lowest in the night; highest in the summer, lowest in the winter. Significant differences were found between AERs measured by the different techniques. The median nighttime AER in all bedrooms across the four seasons was 0.49 h-1 with the active tracer gas technique and 1.20 h-1 with the CO2 method. The average winter AER in the five homes with the passive tracer (0.63 h-1) differed substantially from the corresponding AER measured with the active tracer gas (0.25 h-1). Additionally, we studied the pollutant distribution from one room (source room) and interzonal airflows across the dwellings. The air within a given floor was well mixed, with the average tracer gas concentration in the non-source rooms reaching approximately 70% of the source room concentration. There was less air movement between different floors. The position of the internal doors had a strong influence on the air movement.

The principle objective of the Danish research program ‘Indoor Environment and Children’s Health’ (IECH) was to explore associations between various exposures that children experience in their indoor environments (specifically their homes and daycare centers) and their well-being and health. The targeted health endpoints were allergy, asthma, and certain respiratory symptoms. The study was designed with two stages. In the first stage, a questionnaire survey was distributed to more than 17 000 families with children between the ages of 1 and 5. The questionnaire focused on the children’s health and the environments within the homes they inhabited and daycare facilities they attended. More than 11 000 questionnaires were returned. In the second stage, a subsample of 500 children was selected for more detailed studies, including an extensive set of measurements in their homes and daycare centers and a clinical examination; all clinical examinations were carried out by the same physician. In this study, the methods used for data collection within the IECH research program are presented and discussed. Furthermore, initial findings are presented regarding descriptors of the study population and selected characteristics of the children’s dwellings and daycare centers. Practical Implications This study outlines methods that might be followed by future investigators conducting large-scale field studies of potential connections between various indoor environmental factors and selected health endpoints. Of particular note are (i) the two-stage design – a broad questionnaire-based survey followed by a more intensive set of measurements among a subset of participants who have been selected based on their responses to the questionnaire; (ii) the case–base approach utilized in the stage 2 in contrast to the more commonly used case–control approach; (iii) the inclusion of the children’s daycare environment when conducting intensive sampling to more fully capture the children’s total indoor exposure; and (iv) all clinical examinations conducted by the same physician. We recognize that future investigators are unlikely to fully duplicate the methods outlined in this study, but we hope that it provides a useful starting point in terms of factors that might be considered when designing such a study.

Air change rate (ACR) data obtained from the bedrooms of 500 Danish children and presented in an earlier paper were analyzed in more detail. Questionnaires distributed to the families, home inspections and interviews with the parents provided information about a broad range of residential characteristics and occupant behavior. These were tested in several linear regression models to identify the degree of effect each selected independent variable has on the total ACR. The measured ACRs are summarized by some of the most significant variables such as room volume (higher ACR in smaller rooms), number of people sleeping in the bedroom (higher ACR with more people), average window and door opening habits (higher ACR with more opening), sharing the bedroom with other family members (higher ACR in shared rooms), location of the measured room (higher ACR above ground floor), year of construction (lowest ACR in buildings from early 1970s), observed condensation on the bedroom window (higher ACR at less condensation), etc. The best-fitting model explained 46% of the variability in the air change rates. Variables related to occupant behavior were stronger predictors of ventilation rate (model R 2 = 0.30) than those related to building characteristics (model R 2 = 0.09). Although not perfectly accurate on a room-to-room basis, our best-fitting model may be useful when a rough estimate of the average air change rate for larger study populations is required in future indoor air quality models.

Given the rate at which humans shed their skin (desquamation), skin flakes that contain squalene and cholesterol are anticipated to be major constituents of indoor dust. These compounds have been detected in more than 97% of the dust samples collected from 500 bedrooms and 151 daycare centers of young children living in Odense, Denmark. The mass fractions of squalene in dust were approximately log-normally distributed (homes: GM = 32 μg/g, GSD = 4.3; daycare centers: GM = 11.5 μg/g, GSD = 4.3); those of cholesterol displayed a poorer fit to such a distribution (homes: GM = 625 μg/g, GSD = 3.4; daycare centers: GM = 220 μg/g, GSD = 4.0). Correlations between squalene and cholesterol were weak (r = 0.22). Furthermore, the median squalene-to-cholesterol ratio in dust (~0.05) was more than an order of magnitude smaller than that in skin oil. This implies sources in addition to desquamation (e.g., cholesterol from cooking) coupled, perhaps, with a shorter indoor lifetime for squalene. Estimated values of squalene's vapor pressure, while uncertain, suggest meaningful redistribution from dust to other indoor compartments. We estimate that dust containing squalene at 60 μg/g would contribute about 4% to overall ozone removal by indoor surfaces. This is roughly comparable to the fraction of ozone removal that can be ascribed to reactions with indoor terpenes. Squalene containing dust is anticipated to contribute to the scavenging of ozone in all settings occupied by humans.

More than 30 years after the First International Indoor Climate Symposium, ten researchers from the USA, Slovakia, Sweden, and Denmark gathered to review the current status of indoor environmental research. We initiated our review with discussions during the 1-day meeting and followed that with parallel research and writing efforts culminating with internal review and revision cycles. In this paper, we present our choices for the most important research findings on indoor environmental quality from the past three decades followed by a discussion of the most important research questions in our field today. We then continue with a discussion on whether there are research areas for which we can ‘close the book’ and say that we already know what is needed. Finally, we discuss whether we can maintain our identity in the future or it is time to team up with new partners. Practical Implications In the early years of this field, the accumulated knowledge was small and it was possible for any researcher to acquire a complete understanding. To do so has become impossible today as what we know has grown to exceed the learning capacity of any person. These circumstances challenge us to work collectively to synthesize what we do know and to define clearly what remains to be learned. If we fail to do these things well, we risk repeating research without memory, an inefficiency that we cannot afford.

In this study, the performance, combustion and injection characteristics of a direct injection diesel engine have been investigated experimentally when it was fueled with canola oil methyl ester (COME) and waste (frying) palm oil methyl ester (WPOME). In order to determine the performance and combustion characteristics, the experiments were conducted at constant engine speeds under the full load condition of the engine. The results indicated that when the test engine was fueled with WPOME or COME instead of petroleum based diesel fuel (PBDF), the brake power reduced by 4–5%, while the brake specific fuel consumption increased by 9–10%. On the other hand, methyl esters caused reductions in carbon monoxide (CO) by 59–67%, in unburned hydrocarbon (HC) by 17–26%, in carbon dioxide (CO2) by 5–8%, and smoke opacity by 56–63%. However, both methyl esters produced more nitrogen oxides (NOx) emissions by 11–22% compared with those of the PBDF over the speed range.

The ongoing “Indoor Environment and Children’s Health” (IECH) study investigates the environmental risk factors in homes and their association with asthma and allergy among children aged 1–5 years. As part of the study, the homes of 500 children between 3 and 5 years of age were inspected. The selected children included 200 symptomatic children (cases) and 300 randomly selected children (bases). As part of the inspection, the concentration of carbon dioxide in the bedrooms of the children was continuously measured over an average of 2.5 days. The ventilation rates in the rooms during the nights when the children were sleeping in the room were calculated using a single-zone mass balance for the occupant-generated CO 2 . The calculated air change rates were log-normally distributed ( R 2 > 0.98). The geometric mean of the air change rates in both the case and the base group was 0.46 air changes per hour (h −1 ; geom. SD = 2.08 and 2.13, respectively). Approximately 57% of both cases and bases slept at a lower ventilation rate than the minimum required ventilation rate of 0.5 h −1 in new Danish dwellings. Only 32% of the bedrooms had an average CO 2 concentration below 1000 ppm during the measured nights. Twenty-three percent of the rooms experienced at least a 20-minute period during the night when the CO 2 concentration was above 2000 ppm and 6% of the rooms experienced concentrations above 3000 ppm. The average air change rate was higher with more people sleeping in the room. The air change rate did not change with the increasing outdoor temperature over the 10-week experimental period. The calculation method provides an estimate of the total airflow into the bedroom, including airflows both from outdoors and from adjacent spaces. To study the accuracy of the calculated air change rates and their deviation from the true outside air change rates, we calculated CO 2 concentrations at different given air change rates using an indoor air quality and ventilation model (Contam). Subsequently we applied our calculation procedure to the obtained data. The air change rate calculated from the generated CO 2 concentrations was found to be between 0% and 51% lower than the total air change rate defined in the input variables for the model. It was, however, higher than the true outside air change rate. The relative error depended on the position of the room in relation to the adjacent rooms, occupancy in the adjacent room, the nominal air change rate and room-to-room airflows.

As part of the Danish Indoor Environment and Children’s Health (IECH) study, dust samples were collected from 500 bedrooms and 151 daycare centers of children (ages 3 to 5) living on the island of Fyn. The present paper reports results from the analyses of these samples for five phthalate esters (diethyl phthalate (DEP), di(n-butyl) phthalate (DnBP), di(isobutyl) phthalate (DiBP), butyl benzyl phthalate (BBzP), di(2-ethylhexyl) phthalate (DEHP)) and three PAHs (pyrene, benz[a]anthracene (B[a]A) and benzo[a]pyrene (B[a]P)). The three PAHs and DEHP were detected in dust samples from all sites, while DEP, DnBP, DiBP and BBzP were detected in more than 75% of the bedrooms and more than 90% of the daycare centers. The dust mass-fractions of both phthalates and PAHs were log-normally distributed. With the exception of DEP, the mass-fractions of phthalates in dust were higher in daycare centers than homes; PAH mass-fractions in dust were similar in the two locations. There was no correlation among the different phthalates in either homes or daycare centers. In contrast, the PAH were correlated with one another – more strongly so in homes ( R 2 = 0.80–0.90) than in daycare centers ( R 2 = 0.28–0.45). The dust levels of several phthalates (BBzP, DnBP and DEHP) were substantially lower than those measured in a comparable study conducted 6–7 years earlier in Sweden. Although usage patterns in Denmark differ from those in Sweden, the current results may also reflect a change in the plasticizers that are used in common products including toys. PAH levels were roughly an order of magnitude lower than those measured in Berlin and Cape Cod residences, suggesting that the Danish sites are less impacted by motor vehicle emissions.

Carbon monoxide (CO) and particulate matter (PM2.5) were measured in two reconstructed Danish farmhouses (17–19th century) during two weeks of summer. During the first week intensive measurements were performed while test cooking fires were burned, during the second week the houses were monitored while occupied by guest families. A masonry hearth was located in the middle of each house for open cooking fires and with heating stoves. One house had a chimney leading to the outside over the hearth; in the other, a brickwork hood led the smoke into an attic and through holes in the roof. During the first week the concentration of PM2.5 averaged daily between 138 and 1650 μg m−3 inside the hearths and 21–160 μg m−3 in adjacent living rooms. CO averaged daily between 0.21 and 1.9 ppm in living areas, and up to 12 ppm in the hearths. Highest concentrations were measured when two fires were lit at the same time, which would cause high personal exposure for someone working in the kitchens. 15 min averages of up to 25 400 μg m−3 (PM2.5) and 260 ppm CO were recorded. WHO air quality guidelines were occasionally exceeded for CO and constantly for PM2.5. However, air exchange and air distribution measurements revealed a large draw in the chimney, which ensured a fast removal of wood smoke from the hearth area. The guest families were in average exposed to no more than 0.21 ppm CO during 48 h. Based on a hypothetical time-activity pattern, however, a woman living in this type of house during the 17–19th century would be exposed to daily averages of 1.1 ppm CO and 196 μg m−3 PM2.5, which exceeds WHO guideline for PM2.5, and is comparable to what is today observed for women in rural areas of developing countries.

As ventilation filters accumulate particles removed from the airstream, they become emitters of sensory pollutants that degrade indoor air quality. Previously we demonstrated that an F7 bag-type filter that incorporates activated carbon (a “combination filter”) reduces this adverse effect compared to an equivalent filter without carbon. The aim of the present study was to examine how the amount of activated carbon (AC) used in combination filters affects their ability to remove both sensory offending pollutants and ozone. A panel evaluated the air downstream of four different filters after each had continuously filtered outdoor suburban air over a period of 6 months. Interim assessments (mid-term evaluation) were performed after 3 months. During both assessments, four unused filters, identical in type to the loaded filters, were also evaluated. The evaluated filters included a conventional F7 fiberglass filter and three modifications of a bag-type fiberglass combination filter: the “Heavy” corresponded to a commercially available filter containing 400 g of carbon per square meter of filter area, the “Medium” contained half as much carbon (200 g/m 2 ), and the “Light” contained a quarter as much carbon (100 g/m 2 ). Each filter was weighed at the beginning of the soiling period and after 3 and 6 months of service. Additionally, up- and down-stream ozone concentrations and filter pressure drops were measured monthly. Following 6 months of service, the air downstream of each of the combination filters was judged to be significantly better than the air downstream of the 6-month-old F7 filter, and was comparable to that from an unused F7 filter. Additionally, the combination filters removed more ozone from the air than the F7 filter, with their respective fractional removal efficiencies roughly scaling with their carbon content.

Estimates of costs and the corresponding benefits of particle filtration have been derived for a standard office building. Reduction in occupants’ exposure to particles during their workday is anticipated to reduce their morbidity and mortality. Filtration may also reduce the costs associated with building and HVAC cleaning. Conversely, losses of occupant productivity due to sensory offending pollutants emitted from used ventilation filters can lead to significant economic losses. The results of the present analysis are strongly dependent on several key input parameters; consequently, the sensitivity of the results to these parameters was evaluated as part of this study. The study also acknowledges that the benefits-to-costs ratio depends on the perspective of the stakeholder: the employer renting the building is impacted by occupant performance and building energy costs; the building owner is impacted by maintenance of the building and its HVAC system; society is impacted by the employees’ health and welfare. Regardless of perspective, particle filtration is anticipated to lead to annual savings significantly exceeding the running costs for filtration. However, economic losses resulting from even a small decrease in productivity caused by sensory pollutants emitted from used ventilation filters have the potential to substantially exceed the annual economic benefits of filtration. Further studies are required to determine if meaningful benefits can be obtained from more frequent filter replacement or application of different filtration techniques that limit the emission of offending pollutants into the ventilation air.

Ninety-nine young-adult subjects of both genders were randomly assigned to four groups. One group performed simulated office work for two hours in a set of poor environmental conditions, with overhead fluorescent lighting, recorded traffic noise from a busy street, 27°C (80.6°F) operative temperature, supply air polluted by emissions from linoleum, recorded open office noise, and almost no daylight. The realistic annual cost of improving each of the six conditions was estimated and expressed as a percentage of the total sum of the cost of improving conditions. The modifications included improved lighting, barely audible traffic noise, operative temperature of 22°C (71.6°F), clean air, quiet, and a daylit view out. A second group briefly experienced all 12 conditions and individually selected the improvements they preferred, up to a 50% budget. A third group of subjects was randomly paired with each of the subjects from the second group, and each pair was exposed to the conditions selected by the second-gr...

The benefits of using supply air filters in ventilationand air-conditioning units are well known (Fisk et al.,2002). However, used filters can emit sensory pollu-tants, which degrade the perceived quality of theventilation air (Alm, 2001; Clausen, 2004; Pasanen,1998; Pasanen et al., 1994; Pejtersen, 1996; Pejtersenet al., 1989). At the same time they can contribute tosick building syndrome (SBS) symptoms (Clausenet al., 2002) and negatively impact occupant perfor-mance (Wargocki et al., 2004a; Wyon et al., 2000).Within 6 months of operation, the surface area ofparticles captured by an outdoor air filter can be twoorders of magnitude larger than its cross-sectional area(Weschler, 2003). Various organic compounds such asthose emitted by combustion processes or vegetationare associated with the captured particles. Some ofthese can desorb into the airstream while others canreact with ozone contained in the air passing throughAbstract Used ventilation filters are a major source of sensory pollutants in airhandling systems. The objective of the present study was to evaluate the neteffect that different combinations of filters had on perceived air quality after5 months of continuous filtration of outdoor suburban air. A panel of 32 sub-jects assessed different sets of used filters and identical sets consisting of newfilters. Additionally, filter weights and pressure drops were measured at thebeginning and end of the operation period. The filter sets included single EU5and EU7 fiberglass filters, an EU7 filter protected by an upstream pre-filter(changed monthly), an EU7 filter protected by an upstream activated carbon(AC) filter, and EU7 filters with an AC filter either downstream or bothupstream and downstream. In addition, two types of stand-alone combinationfilters were evaluated: a bag-type fiberglass filter that contained AC and asynthetic fiber cartridge filter that contained AC. Air that had passed throughused filters was most acceptable for those sets in which an AC filter was useddownstream of the particle filter. Comparable air quality was achieved with thestand-alone bag filter that contained AC. Furthermore, its pressure drop chan-ged very little during the 5 months of service, and it had the added benefit ofremoving a large fraction of ozone from the airstream. If similar results areobtained over a wider variety of soiling conditions, such filters may be a viablesolution to a long recognized problem.

The sensory pollutants emitted by loaded ventilation filters are assumed to include products formed via oxidation of organics associated with captured particles. In this study, experiments were performed that used either particle production or ozone removal as probes to further improve our understanding of such processes. The measured ratio of downstream to upstream submicron particle concentrations increased when ozone was added to air passing through samples from loaded particle filters. Such an observation is consistent with low volatility oxidation products desorbing from the filter and subsequently partitioning between the gas phase and the surface of particles that have passed through the filter, including particles that were previously too small (

The presence of the fungal genus Chaetomium and its secondary metabolites in indoor environments is suspected to have a negative impact on human health and wellbeing. About 200 metabolites have been currently described from Chaetomium spp., but only the bioactive compound group, chaetoglobosins, have been screened for, and thus detected in buildings. In this study, we used a liquid chromatography-high resolution mass spectrometry approach to screen both artificially and naturally infected building materials for all the Chaetomium metabolites described in the literature. Pure agar cultures were also investigated in order to establish differences between metabolite production in vitro and on building materials as well as comparison to non-indoor reference strains. On building materials six different chaetoglobosins were detected in total concentrations of up to 950 mg/m2 from C. globosum along with three different chaetoviridins/chaetomugilins in concentrations up to 200 mg/m2. Indoor Chaetomium spp. preferred wood-based materials over gypsum, both in terms of growth rate and metabolite production. Cochliodones were detected for the first time on all building materials infected by both C. globosum and C. elatum, and are thus candidates as Chaetomium biomarkers. No sterigmatocystin was produced by Chaetomium spp. from indoor environment.

Studies in rodents indicate that phthalates can function as adjuvants, increasing the potency of allergens. Meanwhile, epidemiological studies have produced inconsistent findings regarding relationships between phthalate exposures and allergic disease in humans. The present study examined phthalate exposure and allergic sensitization in a large group of 3-5 year old children: 300 random controls and 200 cases with asthma, rhinoconjunctivitis or atopic dermatitis as reported in questionnaires. The children were clinically examined to confirm their health status. Blood samples were analyzed for IgE sensitization to 20 allergens. Adjusted logistic regressions were used to look for associations between phthalate exposure indicators (mass fractions in dust from children's homes and daycares, metabolites in urine, and estimated daily indoor intakes from dust ingestion, inhalation and dermal absorption) and sensitization and allergic disease. No direct associations were found between phthalate exposures and asthma, rhinoconjunctivitis or atopic dermatitis. However, among children with these diseases, there were significant associations between non-dietary exposures to DnBP, BBzP and DEHP in the indoor environment (mass fractions in dust or daily indoor intakes from dust ingestion, inhalation and dermal absorption) and allergic sensitization. Some exposure pathways were more strongly associated with sensitization than others, although the results are not conclusive and require confirmation. A number of the associations depended on accounting for a child's exposure in more than one environment (i.e., daycare facility as well as home). Significant associations were not observed between phthalate metabolites in urine, which reflected exposure from diet as well as indoor pathways, and allergic sensitization.

UNLABELLED Three air temperatures (22/26/30 degrees C) and two acoustic conditions-quiet (35 dBA) or open-plan office noise (55 dBA)-were established in an office. Thirty subjects aged 18-29 years (16 male), clothed for thermal neutrality at 22 degrees C, performed simulated office work for 3 h under all six conditions. Many more (68% vs. 4%) were dissatisfied with noise in the noise condition (P < 0.01). Warmth decreased thermal acceptability (P < 0.001) and perceived air quality (P < 0.01) and increased odour intensity (P < 0.05) and stuffiness (P < 0.01). After 2 h, some forehead sweating was observed on 4, 36 and 76% of subjects (P < 0.001) at 22, 26 and 30 degrees C, while 0, 21 and 65% felt "warm" (P < 0.001). Raised temperature increased eye, nose and throat irritation (P < 0.05), headache intensity (P < 0.05), difficulty in thinking clearly (P < 0.01) and concentrating (P < 0.01), and decreased self-estimated performance (P < 0.001). Noise increased fatigue (P < 0.05) and difficulty in concentrating (P < 0.05) but did not interact with thermal effects on subjective perception. In an addition task, noise decreased workrate by 3% (P < 0.05), subjects who felt warm made 56% more errors (P < 0.05) and there was a noise-temperature interaction (P < 0.01): the effect of warmth on errors was less in the noise condition. Typing speed (P < 0.05) and reading speed (P < 0.05) were higher in noise. PRACTICAL IMPLICATIONS This paper demonstrates that open office noise distraction, even at the realistic level of 55 dBA, increases fatigue and has many negative effects on the performance of office work, as does a moderately warm air temperature. These findings may be used to provide economic justification for the provision of private offices and air temperature control in hot weather. The additional finding that noise distraction and heat stress can sometimes counteract each other in the short term is of academic interest only, as they both increase subjective distress and fatigue. In practice, neither should be deliberately introduced to counteract the other.

UNLABELLED The presence of a used filter in a ventilation system can have an adverse impact on perceived air quality, SBS symptoms, and performance of office work. The present paper reviews the studies leading to this conclusion and discusses recent work that has been performed in a search for the mechanisms involved. One promising hypothesis involves chemical reactions on the surface of the collected particles in the formation of noxious compounds. Finally, a discussion on engineering solutions is presented. PRACTICAL IMPLICATIONS Loaded particle filters provide a significant source of air pollution. To counteract this, filters should be changed frequently or an alternative method of removing particles from the air should be applied.

From 1998 to 2000, a European project named AIRLESS was conducted by 12 institutes, universities and companies from seven European countries. The objective was to develop strategies, principles and protocols to improve and control the performance of HVAC-systems and its components for incorporation in codes and guidelines. The first step was to define air pollution caused by and/or originating from HVAC-systems, to investigate ways to prevent this pollution and to define strategies to keep this pollution away. A summary of this first phase of the AIRLESS project is presented.

Phthalate esters are among the most ubiquitous of indoor pollutants and have been associated with various adverse health effects. In the present study we assessed the cross-sectional association between eight different phthalate metabolites in urine and allergic disease in young children. As part of the Danish Indoor Environment and Children's Health study, urine samples were collected from 440 children aged 3-5 years, of whom 222 were healthy controls, 68 were clinically diagnosed with asthma, 76 with rhinoconjunctivitis and 81 with atopic dermatitis (disease subgroups are not mutually exclusive; some children had more than one disease). There were no statistically significant differences in the urine concentrations of phthalate metabolites between cases and healthy controls with the exception of MnBP and MECPP, which were higher in healthy controls compared with the asthma case group. In the crude analysis MnBP and MiBP were negatively associated with asthma. In the analysis adjusted for multiple factors, only a weak positive association between MEP in urine and atopic dermatitis was found; there were no positive associations between any phthalate metabolites in urine and either asthma or rhinoconjunctivitis. These findings appear to contradict earlier studies. Differences may be due to higher exposures to certain phthalates (e.g., BBzP) via non-dietary pathways in earlier studies, phthalates serving as surrogates for an agent associated with asthma (e.g., PVC flooring) in previous studies but not the present study or altered cleaning habits and the use of "allergy friendly" products by parents of children with allergic disease in the current study in contrast to studies conducted earlier.

Previous studies, often using data from questionnaires, have reported associations between various characteristics of indoor environments and allergic disease. The aim of this study has been to investigate possible associations between objectively assessed indoor environmental factors and clinically confirmed asthma, rhinoconjunctivitis, and atopic dermatitis. The study is a cross-sectional case-control study of 500 children aged 3-5 years from Odense, Denmark. The 200 cases had at least two parentally reported allergic diseases, while the 300 controls were randomly selected from 2835 participating families. A single physician conducted clinical examinations of all 500 children. Children from the initially random control group with clinically confirmed allergic disease were subsequently excluded from the control group and admitted in the case group, leaving 242 in the healthy control group. For most children, specific IgE's against various allergens were determined. In parallel, dust samples were collected and air change rates were measured in the children's bedrooms. The dust samples were analyzed for phthalate esters, polycyclic aromatic hydrocarbons (PAH), nicotine, and various allergens. Among children diagnosed with asthma, concentrations of nicotine were higher (P

Editorial for Indoor Air (Vol. 24, Issue 5, October 2014) WW Nazaroff et al. ISIAQ Academy Awards 2014 The 13 th International Conference on Indoor Air Quality and Climate (Indoor Air 2014) was convened in Hong Kong during the week of 7-12 July 2014. Professor Yuguo Li served as the Conference President. One of many highlights during the conference was the presentation of awards from the ISIAQ Academy of Fellows, which occurred during the opening plenary session. These awards celebrate high achievements in the indoor air sciences. As described in an earlier editorial (Nazaroff, 2012a), the ISIAQ Academy of Fellows has its origins in the creation of the International Academy of Indoor Air Sciences in 1991. In 2005, that organization was reconstituted as a part of the International Society of Indoor Air Quality and Climate (ISIAQ). The ISIAQ Academy of Fellows is an international, multidisciplinary, scientific, honorific organization established to promote scholarship in the indoor environment and building sciences. The awards program, which is featured at all Indoor Air conferences, is a core activity of the Academy. Best Paper Awards The best-papers jury reviewed 143 papers that were published in Indoor Air during the years 2011-2013. Papers were judged for originality, scientific and technical content, and importance. In addition to these criteria, writing style was taken into consideration. The three winning papers each describe the results of field studies. It is noteworthy that two of these papers address what the jury considers to be the most important indoor air quality problem: exposures and health risks associated with indoor biomass cooking. In chronological order, these are the winning papers: • Haverinen-Shaughnessy, U., Moschandreas, D.J. and Shaughnessy, R.J. (2011) Association between substandard classroom ventilation rates and students’ academic achievement, Indoor Air 21, 121-131. This study used a cost-effective design to explore the relationship between classroom ventilation (estimated from CO 2 measurement in one classroom per school) and fifth-grade students passing mandatory standardized tests as a measure of student academic performance. The results are policy relevant, suggesting that increasing the ventilation rates in the many underventilated classrooms toward the recommended guideline values could translate into improved academic achievement of the students. The paper received high scores for originality and importance. • Dutta, A., Mukherjee, B., Das, D., Banerjee, A. and Ray, M.R. (2011) Hypertension with elevated levels of oxidized low-density lipoprotein and anticardiolipin antibody in the circulation of premenopausal Indian women chronically exposed to biomass smoke during cooking, Indoor Air 21, 165-176. In this well-designed study it is shown that poor women in rural India have a high risk of developing cardiovascular diseases. The methods employed are state-of-the-art, including numerous objective measures or signs of health. The paper leaves the reader with a solid impression of excellent science and craftsmanship. The paper scored high in all of the three main criteria. • Hawley, B. and Volckens, J. (2013) Proinflammatory effects of cookstove emissions on human bronchial epithelial cells, Indoor Air 23, 4-13. Another well designed study on effects of biomass cookstoves. The study uses human bronchial epithelial cells to assess pro- inflammatory effects with exposure to either traditional or improved (cleaner burning) cookstove emissions. The results support other evidence indicating that efficient cookstoves can reduce the health burden associated with exposure to household pollution from combustion of solid biomass fuels. The study uses multiple assays to assess effects; the work

Perceived air quality, Sick Building Syndrome (SBS) symptoms and productivity were studied in a normally furnished office space (108 m3) ventilated with an outdoor airflow of 3, 10 or 30 L/s per person, corresponding to an air change rate of 0.6, 2 or 6 h-1. The temperature of 22 degrees C, the relative humidity of 40% and all other environmental parameters remained unchanged. Five groups of six female subjects were each exposed to the three ventilation rates, one group and one ventilation rate at a time. Each exposure lasted 4.6 h and took place in the afternoon. Subjects were unaware of the intervention and remained thermally neutral by adjusting their clothing. They assessed perceived air quality and SBS symptoms at intervals, and performed simulated normal office work. Increasing ventilation decreased the percentage of subjects dissatisfied with the air quality (P < 0.002) and the intensity of odour (P < 0.02), and increased the perceived freshness of air (P < 0.05). It also decreased the sensation of dryness of mouth and throat (P < 0.0006), eased difficulty in thinking clearly (P < 0.001) and made subjects feel generally better (P < 0.0001). The performance of four simulated office tasks improved monotonically with increasing ventilation rates, and the effect reached formal significance in the case of text-typing (P < 0.03). For each two-fold increase in ventilation rate, performance improved on average by 1.7%. This study shows the benefits for health, comfort and productivity of ventilation at rates well above the minimum levels prescribed in existing standards and guidelines. It confirms the results of a previous study in the same office when the indoor air quality was improved by decreasing the pollution load while the ventilation remained unchanged.

Acceptability of clean air and air polluted by building materials was studied in climate chambers with different levels of air temperature and humidity in the ranges 18–28°C and 30-70% relative humidity (RH). The acceptability of the air quality immediately after entering a chamber and during the following 20-min whole-body exposure was assessed by 36 untrained subjects who maintained thermal neutrality by modifying their clothing. The results confirm the significant decrement of the acceptability with increasing temperature and humidity, as shown in a previous study with facial exposures. The odour intensity was found to be independent of temperature and humidity. A linear relation between acceptability and enthalpy of air was again observed by this experiment. No significant difference was observed between the immediate acceptability and the acceptability during the following 20-min exposure, i.e., no adaptation took place. Both the immediate assessment of acceptability and the assessments during the 20-min exposure were independent of the air temperature and humidity to which the subjects were exposed before entering the chamber. The results further indicate that a notable decrement of the ventilation requirement may be achieved by maintaining a moderate enthalpy of air in spaces.

The amount of volatile organic compounds (VOCs) in indoor air, usually called TVOC (total volatile organic compounds), has been measured using different definitions and techniques which yield different results. This report recommends a definition of TVOC referring to a specified range of VOCs and it proposes a method for the measurement of this TVOC entity. Within the specified range, the measured concentrations of identified VOCs (including 64 target compounds) are summed up, concentrations of non-identified compounds in toluene equivalents are added and, together with the identified VOCs, they give the TVOC value. The report reviews the TVOC concept with respect to its usefulness for exposure assessment and control and for the prediction of health or comfort effects. Although the report concludes that at present it is not possible to use TVOC as an effect predictor, it affirms the usefulness of TVOC for characterizing indoor pollution and for improving source control as required from the points of view of health, comfort, energy efficiency and sustainability.

Particle number (PN) concentrations (10-300 nm in size) were continuously measured over a period of ~45 h in 56 residences of nonsmokers in Copenhagen, Denmark. The highest concentrations were measured when occupants were present and awake (geometric mean, GM: 22.3 × 10(3) cm(-3)), the lowest when the homes were vacant (GM: 6.1 × 10(3) cm(-3)) or the occupants were asleep (GM: 5.1 × 10(3) cm(-3)). Diary entries regarding occupancy and particle related activities were used to identify source events and apportion the daily integrated exposure among sources. Source events clearly resulted in increased PN concentrations and decreased average particle diameter. For a given event, elevated particle concentrations persisted for several hours after the emission of fresh particles ceased. The residential daily integrated PN exposure in the 56 homes ranged between 37 × 10(3) and 6.0 × 10(6) particles per cm(3)·h/day (GM: 3.3 × 10(5) cm(-3)·h/day). On average, ~90% of this exposure occurred outside of the period from midnight to 6 a.m. Source events, especially candle burning, cooking, toasting, and unknown activities, were responsible on average for ~65% of the residential integrated exposure (51% without the unknown activities). Candle burning occurred in half of the homes where, on average, it was responsible for almost 60% of the integrated exposure.

The CISBO centre is a cross-disciplinary research consortium with a goal to improve quality of life throughout the built environment, with a special focus on private housing. The aim of the centre is to develop a scientific basis for decision making, improving the knowledge base and implementation of knowledge into the building with the overall objectives to promote "healthy" buildings This is, in other words, to develop knowledge about buildings and health and to bring this new knowledge as well as established knowledge in use for the benefit of the occupants of the housing stock. To reach the goals we will work in studies describing the exposure occurring in homes in Denmark, we will perform intervention studies on particles and allergens, we will use the existing Danish cohorts, to study effects of indoor air on health, and finally we will perform a series of experiments from indoor chemistry to human exposures. All these studies will be coordinated by the centre, in order to get the most synergy from the research projects initiated under the umbrella of the CISBO centre.

The relative importance of sensory air pollution, thermal load and noise was studied under controlled conditions in two identical environmental chambers. In one chamber subjects were exposed to various levels of either thermal load or poor indoor air quality. For each condition tested in this chamber, the subjects were exposed to a number of noise levels in an adjacent chamber with neutral thermal conditions and good indoor air quality in order to determine a noise level causing the same degree of discomfort. A total of 68 comparisons of the conditions in the two chambers were made by the same group of 16 subjects after one-minute exposure in each chamber. In the operative temperature range of 23–29°C, a 1°C change in operative temperature was found to have the same effect on human comfort as a change in perceived air quality of 2.4 decipol or a change in noise level of 3.9 dB. For levels of perceived air quality up to 10 decipol, a 1 -decipol change in perceived air quality had the same effect on human comfort as a change in noise level of 1.2 dB. A relationship between traffic noise level and percentage dissatisfied was established

Five-hundred Danish families with children aged 3 to 5 years were included in a study of the indoor environments in their dwellings and their children's kindergartens, the aim being to assess the influence of the exposures that occur in both these environments on the children's health. Exposures were evaluated based on parents' reports of dwelling characteristics and inspectors' reports derived from visits to the dwellings. Parents' reports of moisture problems seemed to be associated with cases (asthma, rhinitis, and eczema) more than bases, whereas there was no statistically significant association between inspector observations of moisture, odor, or mould and three health symptoms (wheezing, rhinitis, and eczema).

Used filters can be a strong sensory pollutant source. Oxidation processes, especially those initiated by ozone, may contribute to the pollutants emitted from such filters. In the present study, ozone was added to the airstream passing through used ventilation filters. Two flow rates were examined. While the upstream ozone concentration was approximately 75 ppb, the concentrations downstream of the filter were initially 35-50% lower. However, within an hour downstream concentrations were only 5-10% lower than those upstream. These filter samples were then placed for 48 h in nitrogen, ambient air containing less than 5 ppb ozone, or ambient air at an elevated temperature. This resulted in partial regeneration of the ozone removal capability of the filter. In analogous experiments, lower ozone removal occurred when the filter samples were first ventilated for 24 h with ozone-free air before making the measurements. Samples from a new filter removed