14 results on '"Genot B"'
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2. Cas particuliers d'isothermes d'adsorption à marches et application à l'étude des phases superficielles
- Author
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Genot, B., primary and Duval, X., additional
- Published
- 1972
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3. Étude de la chimisorption de O 2 À 77 K à l'aide d'isothermes d'adsorption physique à marches : Systèmes CdOKr et CdOCF 4
- Author
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Génot, B.
- Published
- 1974
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4. Functional stress responses in Glaucophyta: Evidence of ethylene and abscisic acid functions in Cyanophora paradoxa.
- Author
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Genot B, Grogan M, Yost M, Iacono G, Archer SD, and Burns JA
- Abstract
Glaucophytes, an enigmatic group of freshwater algae, occupy a pivotal position within the Archaeplastida, providing insights into the early evolutionary history of plastids and their host cells. These algae possess unique plastids, known as cyanelles that retain certain ancestral features, enabling a better understanding of the plastid transition from cyanobacteria. In this study, we investigated the role of ethylene, a potent hormone used by land plants to coordinate stress responses, in the glaucophyte alga Cyanophora paradoxa. We demonstrate that C. paradoxa produces gaseous ethylene when supplied with exogenous 1-aminocyclopropane-1-carboxylic acid (ACC), the ethylene precursor in land plants. In addition, we show that cells produce ethylene natively in response to abiotic stress, and that another plant hormone, abscisic acid (ABA), interferes with ethylene synthesis from exogenously supplied ACC, while positively regulating reactive oxygen species (ROS) accumulation. ROS synthesis also occurred following abiotic stress and ACC treatment, possibly acting as a second messenger in stress responses. A physiological response of C. paradoxa to ACC treatment is growth inhibition. Using transcriptomics, we reveal that ACC treatment induces the upregulation of senescence-associated proteases, consistent with the observation of growth inhibition. This is the first report of hormone usage in a glaucophyte alga, extending our understanding of hormone-mediated stress response coordination into the Glaucophyta, with implications for the evolution of signaling modalities across Archaeplastida., (© 2024 The Author(s). Journal of Eukaryotic Microbiology published by Wiley Periodicals LLC on behalf of International Society of Protistologists.)
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- 2024
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5. Transcriptome sequencing of seven deep marine invertebrates.
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Burns JA, Daniels J, Becker KP, Casagrande D, Roberts P, Orenstein E, Vogt DM, Teoh ZE, Wood R, Yin AH, Genot B, Wood RJ, Katija K, Phillips BT, and Gruber DF
- Subjects
- Animals, Pacific Ocean, Aquatic Organisms genetics, Sequence Analysis, RNA, Transcriptome, Invertebrates genetics
- Abstract
We present 4k video and whole transcriptome data for seven deep-sea invertebrate animals collected in the Eastern Pacific Ocean during a research expedition onboard the Schmidt Ocean Institute's R/V Falkor in August of 2021. The animals include one jellyfish (Atolla sp.), three siphonophores (Apolemia sp., Praya sp., and Halistemma sp.), one larvacean (Bathochordaeus mcnutti), one tunicate (Pyrosomatidae sp.), and one ctenophore (Lampocteis sp.). Four of the animals were sequenced with long-read RNA sequencing technology, such that the reads themselves define a reference assembly for those animals. The larvacean tissues were successfully preserved in situ and has paired long-read reference data and short read quantitative transcriptomic data for within-specimen analyses of gene expression. Additionally, for three animals we provide quantitative image data, and a 3D model for one siphonophore. The paired image and transcriptomic data can be used for species identification, species description, and reference genetic data for these deep-sea animals., (© 2024. The Author(s).)
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- 2024
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6. An in situ digital synthesis strategy for the discovery and description of ocean life.
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Burns JA, Becker KP, Casagrande D, Daniels J, Roberts P, Orenstein E, Vogt DM, Teoh ZE, Wood R, Yin AH, Genot B, Gruber DF, Katija K, Wood RJ, and Phillips BT
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- Animals, Oceans and Seas, Water, Gelatin, Zooplankton genetics, Robotics
- Abstract
Revolutionary advancements in underwater imaging, robotics, and genomic sequencing have reshaped marine exploration. We present and demonstrate an interdisciplinary approach that uses emerging quantitative imaging technologies, an innovative robotic encapsulation system with in situ RNA preservation and next-generation genomic sequencing to gain comprehensive biological, biophysical, and genomic data from deep-sea organisms. The synthesis of these data provides rich morphological and genetic information for species description, surpassing traditional passive observation methods and preserved specimens, particularly for gelatinous zooplankton. Our approach enhances our ability to study delicate mid-water animals, improving research in the world's oceans.
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- 2024
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7. How cereal flours, starters, enzymes, and process parameters affect the in vitro digestibility of sourdough bread.
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Costantini A, Da Ros A, Nikoloudaki O, Montemurro M, Di Cagno R, Genot B, Gobbetti M, and Giuseppe Rizzello C
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- Edible Grain metabolism, Fermentation, Starch metabolism, Triticum metabolism, Bread analysis, Flour analysis
- Abstract
Digestibility of leavened baked goods relies on multiple factors: starch bioavailability and protein hydrolysis during food processing and digestion, presence of antinutritional factors, and satiety and gastrointestinal symptoms after intake. Several studies highlighted that bread digestibility might be positively affected by long-time sourdough fermentation. Nevertheless, most research is focused on single factors and their effect on digestibility, excluding the potential complementary effects of more than one factor. In this work, a multitude of factors influencing the the in vitro starch and protein digestibility and predicted glycemic index were assessed simultaneously. Forty-six different breads made with various raw material/ingredients (flour, enzymes, lactic acid bacteria cytoplasmic extracts and gluten), type of sourdoughs (fresh or commercial liquid or dried), strains of lactic acid bacteria and yeasts, and time and temperature of fermentation, were preliminarily investigated. Further selection of optimal conditions was based on statistical analysis and final breads were further characterized for their peptide profiles, total free amino acids and quality indexes of the digestible protein fraction. Among the factors considered, results identified a claimed in vitro digestibility for breads made with whole wheat, spelt, and rye flours, obtained with lactic acid bacteria strains selected for the peptidase activity, added of fungal proteases, and fermented at the optimal temperature of 37 °C., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
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- 2022
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8. MPK3 and MPK6 control salicylic acid signaling by up-regulating NLR receptors during pattern- and effector-triggered immunity.
- Author
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Lang J, Genot B, Bigeard J, and Colcombet J
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- Plant Immunity genetics, Salicylic Acid metabolism, Signal Transduction genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism
- Abstract
Arabidopsis thaliana mitogen-activated protein kinases 3 and 6 (MPK3/6) are activated transiently during pathogen-associated molecular pattern-triggered immunity (PTI) and durably during effector-triggered immunity (ETI). The functional differences between these two kinds of activation kinetics and how they coordinate the two layers of plant immunity remain poorly understood. Here, by suppressor analyses, we demonstrate that ETI-mediating nucleotide-binding domain leucine-rich repeat receptors (NLRs) and the NLR signaling components NDR1 and EDS1 can promote the salicylic acid sector of defense downstream of MPK3 activity. Moreover, we provide evidence that both sustained and transient MPK3/6 activities positively control the expression of several NLR genes, including AT3G04220 and AT4G11170. We further show that NDR1 and EDS1 contribute to the up-regulation of these two NLRs in both an ETI and a PTI context. Remarkably, whereas in ETI MPK3/6 activities are dependent on NDR1 and EDS1, they are not in PTI, suggesting crucial differences in the two signaling pathways. Finally, we demonstrate that expression of the NLR AT3G04220 is sufficient to induce expression of defense genes from the salicylic acid branch. Overall, this study expands our knowledge of MPK3/6 functions during immunity and provides new insights into the intricate interplay of PTI and ETI., (© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2022
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9. Organismal and cellular interactions in vertebrate-alga symbioses.
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Yang H, Genot B, Duhamel S, Kerney R, and Burns JA
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- Animals, Eukaryota physiology, Mammals, Photosynthesis, Vertebrates, Plants, Symbiosis physiology
- Abstract
Photosymbioses, intimate interactions between photosynthetic algal symbionts and heterotrophic hosts, are well known in invertebrate and protist systems. Vertebrate animals are an exception where photosynthetic microorganisms are not often considered part of the normal vertebrate microbiome, with a few exceptions in amphibian eggs. Here, we review the breadth of vertebrate diversity and explore where algae have taken hold in vertebrate fur, on vertebrate surfaces, in vertebrate tissues, and within vertebrate cells. We find that algae have myriad partnerships with vertebrate animals, from fishes to mammals, and that those symbioses range from apparent mutualisms to commensalisms to parasitisms. The exception in vertebrates, compared with other groups of eukaryotes, is that intracellular mutualisms and commensalisms with algae or other microbes are notably rare. We currently have no clear cell-in-cell (endosymbiotic) examples of a trophic mutualism in any vertebrate, while there is a broad diversity of such interactions in invertebrate animals and protists. This functional divergence in vertebrate symbioses may be related to vertebrate physiology or a byproduct of our adaptive immune system. Overall, we see that diverse algae are part of the vertebrate microbiome, broadly, with numerous symbiotic interactions occurring across all vertebrate and many algal clades. These interactions are being studied for their ecological, organismal, and cellular implications. This synthesis of vertebrate-algal associations may prove useful for the development of novel therapeutics: pairing algae with medical devices, tissue cultures, and artificial ecto- and endosymbioses., (© 2022 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
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- 2022
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10. Commercial Organic Versus Conventional Whole Rye and Wheat Flours for Making Sourdough Bread: Safety, Nutritional, and Sensory Implications.
- Author
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Pontonio E, Arora K, Dingeo C, Carafa I, Celano G, Scarpino V, Genot B, Gobbetti M, and Di Cagno R
- Abstract
Organic farming is gaining a broad recognition as sustainable system, and consumer demand for organic products has increased dramatically in the recent past. Whether organic agriculture delivers overall advantages over conventional agriculture is, however, contentious. Here, the safety, nutritional, and sensory implications of using commercial organic rye, soft, and durum wheat flours rather than conventional-made sourdough bread have been investigated. Culture-dependent and culture-independent approaches were used to explore the microbial architecture of flours and to study their dynamics during sourdough propagation. Besides biochemical features, the main nutritional (amino acid content, asparagine level, and antioxidant activity) characteristics of sourdoughs were investigated, and their effect on the structural, nutritional, and sensory profiles of breads assessed. Overall, the organic farming system led to flours characterized by lower content of asparagine and cell density of Enterobacteriaceae while showing higher concentration of total free amino acids. Differences of the flours mirrored those of sourdoughs and breads. The use of sourdough fermentation guaranteed a further improvement of the flour characteristics; however, a microbial and sensory profile simplification as well as a slight decrease of the biochemical parameters was observed between breads with sourdough after one-cycle fermentation and 10 days of propagation., Competing Interests: BG was employed by company Puratos srl. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer MG declared a past co-authorship with one of the authors, MG, to the handling editor., (Copyright © 2021 Pontonio, Arora, Dingeo, Carafa, Celano, Scarpino, Genot, Gobbetti and Di Cagno.)
- Published
- 2021
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11. Sourdough Fermented Breads are More Digestible than Those Started with Baker's Yeast Alone: An In Vivo Challenge Dissecting Distinct Gastrointestinal Responses.
- Author
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Rizzello CG, Portincasa P, Montemurro M, Di Palo DM, Lorusso MP, De Angelis M, Bonfrate L, Genot B, and Gobbetti M
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- Adult, Female, Gallbladder Emptying physiology, Gastric Emptying physiology, Humans, Male, Postprandial Period physiology, Young Adult, Bread microbiology, Digestion physiology, Fermented Foods microbiology, Lactobacillus plantarum metabolism, Saccharomyces cerevisiae metabolism
- Abstract
As a staple food, bread digestibility deserves a marked nutritional interest. Combining wide-spectrum characterization of breads, in vitro nutritional indices, and in vivo postprandial markers of gastrointestinal function, we aimed at comparing the digestibility of sourdough and baker's yeast breads. Microbiological and biochemical data showed the representativeness of the baker´s yeast bread (BYB) and the two sourdough breads (SB and t-SB, mainly differing for the time of fermentation) manufactured at semi-industrial level. All in vitro nutritional indices had the highest scores for sourdough breads. Thirty-six healthy volunteers underwent an in vivo challenge in response to bread ingestion, while monitoring gallbladder, stomach, and oro-cecal motility. SB, made with moderate sourdough acidification, stimulated more appetite and induced lower satiety. t-SB, having the most intense acidic taste, induced the highest fullness perception in the shortest time. Gallbladder response did not differ among breads, while gastric emptying was faster with sourdough breads. Oro-cecal transit was prolonged for BYB and faster for sourdough breads, especially when made with traditional and long-time fermentation (t-SB), whose transit lasted ca. 20 min less than BYB. Differences in carbohydrate digestibility and absorption determined different post-prandial glycaemia responses. Sourdough breads had the lowest values. After ingesting sourdough breads, which had a concentration of total free amino acids markedly higher than that of BYB, the levels in blood plasma were maintained at constantly high levels for extended time.
- Published
- 2019
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12. Constitutive activity of the Arabidopsis MAP Kinase 3 confers resistance to Pseudomonas syringae and drives robust immune responses.
- Author
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Lang J, Genot B, Hirt H, and Colcombet J
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- Arabidopsis genetics, Disease Resistance, Gene Expression Regulation, Plant, Models, Biological, Plant Diseases immunology, Plant Diseases microbiology, Arabidopsis enzymology, Arabidopsis microbiology, Arabidopsis Proteins metabolism, Mitogen-Activated Protein Kinase Kinases metabolism, Plant Immunity, Pseudomonas syringae physiology
- Abstract
Mitogen Activated Protein Kinases (MAPKs) are known to be important mediators of plant responses to biotic and abiotic stresses. In a recent report, we enlarged the understanding of the Arabidopsis thaliana MPK3 functions showing that the expression of a constitutively active (CA) form of the protein led to auto-immune phenotypes. CA-MPK3 plants are dwarf and display defense responses that are characterized by the accumulation of salicylic acid and phytoalexins as well as by the upregulation of several defense genes. Consistently with these data, we present here results demonstrating that, compared with wild type controls, CA-MPK3 plants are more resistant to the hemibiotrophic pathogen Pseudomonas syringae DC3000. Based on our previous work, we also discuss the mechanisms of robust plant immunity controlled by sustained MPK3 activity, focusing especially on the roles of disease resistance proteins.
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- 2017
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13. MAPK-triggered chromatin reprogramming by histone deacetylase in plant innate immunity.
- Author
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Latrasse D, Jégu T, Li H, de Zelicourt A, Raynaud C, Legras S, Gust A, Samajova O, Veluchamy A, Rayapuram N, Ramirez-Prado JS, Kulikova O, Colcombet J, Bigeard J, Genot B, Bisseling T, Benhamed M, and Hirt H
- Subjects
- Flagellin immunology, Histones metabolism, Immunity, Innate, Phosphorylation, Stress, Physiological, Arabidopsis immunology, Arabidopsis Proteins metabolism, Chromatin physiology, Chromatin Assembly and Disassembly, Histone Deacetylases metabolism, Mitogen-Activated Protein Kinase Kinases metabolism, Plant Immunity
- Abstract
Background: Microbial-associated molecular patterns activate several MAP kinases, which are major regulators of the innate immune response in Arabidopsis thaliana that induce large-scale changes in gene expression. Here, we determine whether microbial-associated molecular pattern-triggered gene expression involves modifications at the chromatin level., Results: Histone acetylation and deacetylation are major regulators of microbial-associated molecular pattern-triggered gene expression and implicate the histone deacetylase HD2B in the reprogramming of defence gene expression and innate immunity. The MAP kinase MPK3 directly interacts with and phosphorylates HD2B, thereby regulating the intra-nuclear compartmentalization and function of the histone deacetylase., Conclusions: By studying a number of gene loci that undergo microbial-associated molecular pattern-dependent activation or repression, our data reveal a mechanistic model for how protein kinase signaling directly impacts chromatin reprogramming in plant defense.
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- 2017
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14. Constitutively Active Arabidopsis MAP Kinase 3 Triggers Defense Responses Involving Salicylic Acid and SUMM2 Resistance Protein.
- Author
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Genot B, Lang J, Berriri S, Garmier M, Gilard F, Pateyron S, Haustraete K, Van Der Straeten D, Hirt H, and Colcombet J
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- Arabidopsis drug effects, Cell Death drug effects, Enzyme Activation drug effects, Ethylenes metabolism, Flagellin pharmacology, Genes, Plant, Indoles metabolism, Metabolome drug effects, Models, Biological, Mutation genetics, Pathogen-Associated Molecular Pattern Molecules metabolism, Phenotype, Plant Immunity drug effects, Plants, Genetically Modified, Reactive Oxygen Species metabolism, Scopoletin metabolism, Signal Transduction drug effects, Stress, Physiological genetics, Thiazoles metabolism, Arabidopsis enzymology, Arabidopsis immunology, Arabidopsis Proteins metabolism, Carrier Proteins metabolism, Disease Resistance, Mitogen-Activated Protein Kinase Kinases metabolism, Salicylic Acid metabolism
- Abstract
Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thaliana CA-MPK3 plants are dwarfed and display a massive derepression of defense genes associated with spontaneous cell death as well as the accumulation of reactive oxygen species, phytoalexins, and the stress-related hormones ethylene and salicylic acid (SA). Remarkably CA-MPK3 / sid2 and CA-MPK3 / ein2-50 lines, which are impaired in SA synthesis and ethylene signaling, respectively, retain most of the CA-MPK3 -associated phenotypes, indicating that the constitutive activity of MPK3 can bypass SA and ethylene signaling to activate defense responses. A comparative analysis of the molecular phenotypes of CA-MPK3 and mpk4 autoimmunity suggested convergence between the MPK3- and MPK4-guarding modules. In support of this model, CA-MPK3 crosses with summ1 and summ2 , two known suppressors of mpk4 , resulted in a partial reversion of the CA-MPK3 phenotypes. Overall, our data unravel a novel mechanism by which the MAPK signaling network contributes to a robust defense-response system., (© 2017 American Society of Plant Biologists. All Rights Reserved.)
- Published
- 2017
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